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| 1 | New insights into estrogenic regulation of O^6-methylguanine DNA-methyltransferase (MGMT) in human breast cancer cells: Co-degradation of ER-α and MGMT proteins by fulvestrant or O^6-benzylguanine indicates fresh avenues for therapy显示文摘Endocrine therapy using estrogen receptor-α(ER-α) antagonists for attenuating horm2one-driven cell proliferation is a major treatment modality for breast cancers.To exploit any DNA repair deficiencies associated with endocrine therapy,we investigated the functional and physical interactions of ER-α with O^6-methylguanine DNA methyltransferase(MGMT),a unique DNA repair protein that confers tumor resistance to various anticancer alkylating agents.The ER-α-positive breast cancer cell lines(MCF-7,T47D) and ER- negative cell lines(MDAMB-468,MDAMB-231),and established inhibitors of ER-α and MGMT,namely,ICI-182,780(Faslodex) and O^6-benzylguanine,respectively,were used to study MGMT- ER interactions.The MGMT gene promoter was found to harbor one full and two half estrogen-responsive elements(EREs) and two antioxidant-responsive elements(AREs).MGMT expression was upregulated by estrogen,downregulated by tamoxifen in Western blot and promoter-linked reporter assays.Similarly,both transient and stable transfections of Nrf-2(nuclear factor-erythroid 2-related factor-2)increased the levels of MGMT protein and activity 3 to 4-fold reflecting novel regulatory nodes for this drugresistance determinant.Of the different ER-α antagonists tested,the pure anti-estrogen fulvestrant was most potent in inhibiting the MGMT activity in a dose,time and ER-α dependent manner,similar to O^6-benzylguanine.Interestingly,fulvestrant exposure led to a degradation of both ER-α and MGMT proteins and O^6-benzylguanine also induced a specific loss of ER-α and MGMT proteins in MCF-7 and T47 D breast cancer cells with similar kinetics.Immunoprecipitation revealed a specific association of ER-α and MGMT proteins in breast cancer cells.Furthermore,silencing of MGMT gene expression triggered a decrease in the levels of both MGMT and ER-α proteins.The involvement of proteasome in the drug-induced degradation of both proteins was also demonstrated.Fulvestrant enhanced the cytotoxicity of MGMT-targeted alkylating agents,namely,temozolomide and BCNU by 3 to 4-fold in ER-α positive cells,but not in ER-negative cells.We conclude that MGMT and ER-α proteins exist as a complex and are co-targeted for ubiquitin-conjugation and subsequent proteasomal degradation.The findings offer a clear rationale for combining alkylating agents with endocrine therapy. | Ameya Paranjpe Nathan I. Bailes Santhi Konduri George C. Bobustuc Francis Ali-Osman Mohd. A. Yusuf Surendra R. Punganuru Hanumantha Rao Madal Debasish Basak AGM Mostofa Kalkunte S. Srivenugopa | 2016 | The Journal of Biomedical Research2016，30，5： | 5 |
| 2 | Comparison of a plant based natural surfactant with SDS for washing of As(V) from Fe rich soil显示文摘This study explores the possible application of a biodegradable plant based surfactant,obtained from Sapindus mukorossi,for washing low levels of arsenic(As)from an iron(Fe)rich soil.Natural association of As(V)with Fe(III)makes the process difcult.Soapnut solution was compared to anionic surfactant sodium dodecyl sulfate(SDS)in down-flow and a newly introduced suction mode for soil column washing.It was observed that soapnut attained up to 86%efciency with respect to SDS in removing As.Full factorial design of experiment revealed a very good fit of data.The suction mode generated up to 83 kPa pressure inside column whilst down-flow mode generated a much higher pressure of 214 kPa,thus making the suction mode more efcient.Micellar solubilisation was found to be responsible for As desorption from the soil and it followed 1st order kinetics.Desorption rate coefcient of suction mode was found to be in the range of 0.005 to 0.01,much higher than down-flow mode values.Analysis of the FT-IR data suggested that the soapnut solution did not interact chemically with As,ofering an option for reusing the surfactant.Soapnut can be considered as a soil washing agent for removing As even from soil with high Fe content. | Soumyadeep Mukhopadhyay Mohd. Ali Hashim Jaya Narayan Sahu Ismail Yusoff Bhaskar Sen Gupta | 2013 | Journal of Environmental Sciences2013，25，11： | 5 |
| 3 | Adsorption of methylene blue on low-cost adsorbents: A review显示文摘 | Mohd. Rafatullah Othman Sulaiman Rokiah Hashim Anees Ahmad | 2009 | Journal of Hazardous Materials2009，，1： | 3 |
| 4 | Zingiber zerumbet (L.) Smith: A Review of Its Ethnomedicinal, Chemical, and Pharmacological Uses显示文摘 | N. J. Yob S. Mohd. Jofrry M. M. R. Meor. Mohd. Affandi L. K. Teh M. Z. Salleh Z. A. Zakaria | 2011 | Evidence-Based Complementary and Alternative Medicine2011，，： | 2 |
| 5 | A comparative study of biopolymers and alum in the separation and recovery of pulp fibres from paper mill effluent by flocculation显示文摘Recovery of cellulose fibres from paper mill effluent has been studied using common polysaccharides or biopolymers such as Guar gum,Xanthan gum and Locust bean gum as flocculent.Guar gum is commonly used in sizing paper and routinely used in paper making.The results have been compared with the performance of alum,which is a common coagulant and a key ingredient of the paper industry.Guar gum recovered about 3.86 mg/L of fibre and was most effective among the biopolymers.Settling velocity distribution curves demonstrated that Guar gum was able to settle the fibres faster than the other biopolymers;however,alum displayed the highest particle removal rate than all the biopolymers at any of the settling velocities.Alum,Guar gum,Xanthan gum and Locust bean gum removed 97.46%,94.68%,92.39%and 92.46%turbidity of raw effluent at a settling velocity of 0.5 cm/min,respectively.The conditions for obtaining the lowest sludge volume index such as pH,dose and mixing speed were optimised for guar gum which was the most effective among the biopolymers.Response surface methodology was used to design all experiments,and an optimum operational setting was proposed.The test results indicate similar performance of alum and Guar gum in terms of floc settling velocities and sludge volume index.Since Guar gum is a plant derived natural substance,it is environmentally benign and offers a green treatment option to the paper mills for pulp recycling. | Sumona Mukherjee Soumyadeep Mukhopadhyay Agamuthu Pariatamby Mohd. Ali Hashim Jaya Narayan Sahu Bhaskar Sen Gupta | 2014 | Journal of Environmental Sciences2014，26，9： | 2 |
| 6 | Morphological and Inter Simple Sequence Repeat (ISSR) Markers Analyses of Corynespora cassiicola Isolates from Rubber Plantations in Malaysia显示文摘 | Nguyen Anh Nghia Jugah Kadir E. Sunderasan Mohd. Puad Abdullah Adam Malik Suhaimi Napis | 2008 | Mycopathologia2008，，4： | 2 |
| 7 | Melastoma malabathricum (L.) Smith Ethnomedicinal Uses, Chemical Constituents, and Pharmacological Properties: A Review显示文摘 | S. Mohd. Joffry N. J. Yob M. S. Rofiee M. M. R. Meor Mohd. Affandi Z. Suhaili F. Othman A. Md. Akim M. N. M. Desa Z. A. Zakaria Angelo Antonio Izzo | 2011 | Evidence-Based Complementary and Alternative Medicine2011，，： | 1 |
| 8 | Adsorption of methylene blue on low-cost adsorbents: A review显示文摘 | Mohd. Rafatullah Othman Sulaiman Rokiah Hashim Anees Ahmad | 2009 | Journal of Hazardous Materials2009，，1： | 1 |
| 9 | Growth and characterization of glycine picrate—Remarkable second-harmonic generation in centrosymmetric crystal显示文摘 | Mohd. Shakir S.K. Kushwaha K.K. Maurya Manju Arora G. Bhagavannarayana | 2009 | Journal of Crystal Growth2009，，15： | 1 |
| 10 | Potassium dodecatungstocobaltate trihydrate (K 5 CoW 12 O 40 <ce:hsp sp='0.25'/>·<ce:hsp sp='0.25'/>3H 2 O): A mild and efficient reusable catalyst for the synthesis of amidoalkyl naphthols in solution and under solvent-free conditions显示文摘 | Lingaiah Nagarapu Mohd. Baseeruddin Satyender Apuri Srinivas Kantevari | 2007 | Catalysis Communications2007，，11： | 1 |
| 11 | Tissue kallikrein and kininogen levels in fetoplacental tissues from normotensive pregnant women and women with pregnancy-induced hypertension显示文摘 | Mahaneem Mohamed Ernest T. Larmie Harbindar J. Singh Mohd. S. Othman | 2006 | European Journal of Obstetrics and Gynecology2006，，1： | 1 |
| 12 | The Use of FTIR Spectroscopy and Chemometrics for Rapid Authentication of Extra Virgin Olive Oil显示文摘 | Abdul Rohman Y. B. Che Man Farahwahida Mohd. Yusof | 2014 | Journal of the American Oil Chemists’ Society2014，，2： | 1 |
| 13 | Blood lead levels in children of Lucknow, India显示文摘 | MaqusoodAhamed SanjeevVerma ArchanaKumar Mohd. K. J.Siddiqui | 2010 | Environ. Toxicol2010，，： | 1 |
| 14 | Statistical optimization of process parameters for landfill leachate treatment using electro-Fenton technique显示文摘 | Soraya Mohajeri Hamidi Abdul Aziz Mohamed Hasnain Isa Mohammad Ali Zahed Mohd. Nordin Adlan | 2009 | Journal of Hazardous Materials2009，，1： | 1 |
| 15 | Antidiabetic effect of Punica granatum flowers: Effect on hyperlipidemia, pancreatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes显示文摘 | Priyanka Bagri Mohd. Ali Vidhu Aeri Malay Bhowmik Shahnaz Sultana | 2008 | Food and Chemical Toxicology2008，，1： | 1 |
| 16 | Trends in the use of Fenton, electro-Fenton and photo-Fenton for the treatment of landfill leachate显示文摘 | Muhammad Umar Hamidi Abdul Aziz Mohd. Suffian Yusoff | 2010 | Waste Management2010，，11： | 1 |
| 17 | Cooperative Problem-based Learning (CPBL): Framework for Integrating Cooperative Learning and Problem-based Learning显示文摘 | Khairiyah Mohd. Yusof Syed Ahmad Helmi Syed Hassan Mohammad Zamry Jamaludin Nor Farida Harun | 2012 | Procedia - Social and Behavioral Sciences2012，，： | 1 |
| 18 | Application of response surface methodology (RSM) for optimization of ammoniacal nitrogen removal from semi-aerobic landfill leachate using ion exchange resin显示文摘 | Mohammed J.K. Bashir Hamidi Abdul Aziz Mohd Suffian Yusoff Mohd. Nordin Adlan | 2009 | Desalination2009，，1： | 1 |
| 19 | Real traffic-data based evaluation of vehicular traffic environment and state- of-the-art with future issues in location-centric data dissemination for VANETs显示文摘 | Abdul Hafidz Abdul Hanan Mohd. Yazid Idris Omprakash Kaiwartya Mukesh Prasad Rajiv Ratn Shah | 2017 | Digital Communications and Networks2017，3，3： | 1 |
| 20 | DNA binding and cleavage studies of new sulfasalazine-derived dipeptide Zn(II) complex: Validation for specific recognition with 5′–TMP显示文摘 | Sartaj Tabassum Waddhaah M. Al–Asbahy Mohd. Afzal Manal shamsi Farukh Arjmand | 2012 | Journal of Luminescence2012，，11： | 1 |
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