|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase显示文摘包含 methyltransferase 建筑群的象 3 一样的 methyltransferase (METTL3 ) 催化 N6-methyladenosine (m6A ) 形成,一个新奇 epitranscriptomic 标记;然而,这建筑群的性质仍然保持大部分未知。这里,我们报导人的 m6A methyltransferase 建筑群, Wilm 的肿瘤 1 伙伴蛋白质(WTAP ) 和象 14 一样的 methyltransferase (METTL14 ) 的二个新部件。WTAP 与 METTL3 和 METTL14 交往,并且为他们的本地化被要求进在 vivo 与处理因素的 pre-mRNA 并且为 m6A methyltransferase 的催化活动充实的原子点缀。RNA 的多数在 vivo 由 WTAP 和 METTL3 跳了代表包含一致 m6A 主题的 mRNAs。当 WTAP 不在时, METTL3 的 RNA 有约束力的能力强烈被减少,建议 WTAP 可以工作调整到 mRNA 目标的 m6A methyltransferase 建筑群的招募。而且,在有 photoactivatable-ribonucleoside-enhanced crosslinking 和 immunoprecipitation (同等片断) 的联合的 transcriptomic 分析说明那 WTAP 和 METTL3 调整涉及抄写并且 RNA 处理的基因拼接的表示和选择。在 zebrafish 胚胎的调停 Morpholino 的击倒的指向 WTAP 或 METTL3 引起了织物区别缺点并且增加了 apoptosis。这些调查结果提供 WTAP 可以在 m6A methyltransferase 建筑群作为一个规章的子单元工作并且在 RNA 的 epitranscriptomic 规定起一个关键作用的充分证据新陈代谢。 | Xiao-Li Ping Bao-Fa Sun Lu Wang Wen Xiao Xin Yang Wen-Jia Wang Samir Adhikari Yue Shi Ying Lv Yu-Sheng Chen Xu Zhao Ang Li Ying Yang Ujwal Dahal Xiao-Min Lou Xi Liu Jun Huang Wei-Ping Yuan Xiao-Fan Zhu Tao Cheng Yong-Liang Zhao Xinquan Wang Jannie M Rendtlew Danielsen Feng Liu Yun-Gui Yang | 2014 | Cell Research2014，24，2： | 244 |
| 2 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 3 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 4 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 5 | Asymptomatic patients of chronic obstructive pulmonary disease in China显示文摘背景长期的妨碍的肺的疾病(COPD ) 让可变自然历史和并非所有个人跟随一样的功课。这研究试图在 China.Methods 从基于人口的调查识别无征状的 COPD 病人的流行和特征多级式的簇采样策略从七不同省 / 城市在一张人口被使用。所有居民(超过 40 岁) 与一张标准化问询表被会见, spirometry.Post-bronchodilator 在 1 秒(FEV1 ) 强迫了吐气的体积 /forced 不到 70% 的重要能力(FVC ) 被定义为 COPD 的诊断标准。屏蔽的所有 COPD 病人根据长期的呼吸症状的存在或缺席被划分成征兆的组和无征状的组。Socio 人口统计,个人和暴露变量被收集,在从 25 627 采样与 COPD 被诊断的 1668 个病人之中的 analyzed.Results 使遭到,(35.3%) 589 是无征状的。年龄,性别,身体团索引(BMI ) ,农村、城市的分布,吸烟习惯和教育层次在二个组是类似的。64.7% 无征状的病人的一个总数没有 comorbidities。心血管的疾病和肺癌症比无征状的 group.Asymptomatic COPD 组多半是更少在厨房,呼吸疾病的家庭历史和周期性的童年咳嗽里与差的通风介绍的在征兆的 COPD 病人之中是更普通的。无征状的 COPD 病人有的显著地更高的 FEV1 (73.1% 对 61.0%) , FVC (91.9% 对 82.0%) ,和 FEV1/FVC (62.9%vs.58.7%) 的更高的比率(所有 P < 0.001 ) 比征兆的组。更无征状的病人是 underdiagnosed (91.9%vs.54.3% , P < 0.001 ) 比征兆的 patients.Conclusions,这大基于人口的调查在中国证实了无征状的 COPD 病人的高流行。屏蔽测试的呼吸量测定法的更多的使用可能对 COPD 的早察觉重要。 | LU Ming YAO Wan-zhen ZHONG Nan-shan ZHOU Yu-min WANG Chen CHEN Ping KANG Jian HUANG Shao-guang CHEN Bao-yuan WANG Chang-zheng NI Dian-tao WANG Xiao-ping WANG Da-li LIU Sheng-ming Lv Jia-chun SHEN Ning DING Yan-ling RAN Pi-xin | 2010 | Chinese Medical Journal2010，，12： | 17 |
| 6 | Chromosomal level assembly and population sequencing of the Chinese tree shrew genome显示文摘Chinese tree shrews (Tupaia belangeri chinensis) have become an increasingly important experimental animal in biomedical research due to their close relationship to primates. An accurately sequenced and assembled genome is essential for understanding the genetic features and biology of this animal. In this study, we used long-read single-molecule sequencing and high-throughput chromosome conformation capture (Hi-C) technology to obtain a high-qualitychromosome-scale scaffolding of the Chinese tree shrew genome. The new reference genome (KIZ version 2: TS_2.0) resolved problems in presently available tree shrew genomes and enabled accurate identification of large and complex repeat regions, gene structures, and species-specific genomic structural variants. In addition, by sequencing the genomes of six Chinese tree shrew individuals, we produced a comprehensive map of 12.8 M single nucleotide polymorphisms and confirmed that the major histocompatibility complex (MHC) loci and immunoglobulin gene family exhibited high nucleotide diversity in the tree shrew genome. We updated the tree shrew genome database (TreeshrewDB v2.0: http://gffzzb70c77447da74c53hqf5n9k50kqkq6fou.ffgz.tsg.suse.edu.cn) to include the genome annotation information and genetic variations. The new high-quality reference genome of the Chinese tree shrew and the updated TreeshrewDB will facilitate the use of this animal in many different fields of research. | Yu Fan Mao-Sen Ye Jin-Yan Zhang Ling Xu Dan-Dan Yu Tian-Le Gu Yu-Lin Yao Jia-Qi Chen Long-Bao Lv Ping Zheng Dong-Dong Wu Guo-Jie Zhang Yong-Gang Yao | 2019 | Zoological Research2019，40，6： | 15 |
| 7 | Kinetics of the accumulation of group 2 innate lymphoid cells in IL-33-induced and IL-25-induced murine models of asthma:a potential role for the chemokine CXCL16显示文摘ILC2s are implicated in asthma pathogenesis, but little is known about the mechanisms underlying their accumulation in airways.We investigated the time course of ILC2 accumulation in different tissues in murine models of asthma induced by a serial per-nasalchallenge with ovalbumin (OVA), house dust mice (HDM), IL-25 and IL-33 and explored the potential roles of ILC2-attractingchemokines in this phenomenon. Flow cytometry was used to enumerate ILC2s at various time points. The effects of cytokines andchemokines on ILC2 migration were measured in vitro using a chemotaxis assay and in vivo using small animal imaging. Comparedwith saline and OVA challenge, both IL-25 and IL-33 challenge alone induced significant accumulation of ILC2s in the mediastinallymph nodes, lung tissue and bronchoalveolar lavage fluid of challenged animals, but with a distinct potency and kinetics. In vitro,IL-33 and CXCL16, but not IL-25 or CCL25, directly induced ILC2 migration. Small animal in vivo imaging further confirmed that asingle intranasal provocation with IL-33 or CXCL16 was sufficient to induce the accumulation of ILC2s in the lungs followinginjection via the tail vein. Moreover, IL-33-induced ILC2 migration involved the activation of ERK1/2, p38, Akt, JNK and NF-κB, whileCXCL16-induced ILC2 migration involved the activation of ERK1/2, p38 and Akt. These data support the hypothesis that epitheliumderived IL-25 and IL-33 induce lung accumulation of ILC2s, while IL-33 exerts a direct chemotactic effect in this process. AlthoughILC2s express the chemokine receptors CXCR6 and CCR9, only CXCL16, the ligand of CXCR6, exhibits a direct chemoattractanteffect. | Yan Li Shihao Chen Yafei Chi Yiran Yang Xiwen Chen Huating Wang Zhe Lv Jingjing Wang Linjie Yuan Ping Huang Kewu Huang Chris JCorrigan Wei Wang Sun Ying | 2019 | Cellular & Molecular Immunology2019，16，1： | 13 |
| 8 | Treatment of coking wastewater by a novel electric assisted micro-electrolysis filter显示文摘A newly designed electric assisted micro-electrolysis filter(E-ME) was developed to investigate its degradation efficiency for coking wastewater and correlated characteristics. The performance of the E-ME system was compared with separate electrolysis(SE) and micro-electrolysis(ME) systems. The results showed a prominent synergistic effect on COD removal in E-ME systems. Gas chromatography/mass spectrometry(GC–MS) analysis confirmed that the applied electric field enhanced the degradation of phenolic compounds.Meanwhile, more biodegradable oxygen-bearing compounds were detected. SEM images of granular activated carbon(GAC) showed that inactivation and blocking were inhibited during the E-ME process. The effects of applied voltage and initial p H in E-ME systems were also studied. The best voltage value was 1 V, but synergistic effects existed even with lower applied voltage. E-ME systems exhibited some p H buffering capacity and attained the best efficiency in neutral media, which means that there is no need to adjust p H prior to or during the treatment process. Therefore, E-ME systems were confirmed as a promising technology for treatment of coking wastewater and other refractory wastewater. | Ruosong Xie Miaomiao Wu Guangfei Qu Ping Ning Yingying Cai Pei Lv | 2018 | Journal of Environmental Sciences2018，30，4： | 12 |
| 9 | EXPERIMENTAL STUDY OF SCOUR RATE IN CONSOLIDATED COHESIVE SEDIMENT显示文摘The erosion process of a cohesive sediment after consolidation was studied experimentally in a closed conduit system.The test samples investigated in this study are mixtures of sand and clay with variable compositions and different consolidation times.The main concern of this study is the effects of the dry density of the consolidated sediment on scour rate.A scour rate formula is derived and further interpreted based on the experimental results. | TAN Guang-ming JIANG Lei SHU Cai-wen LV Ping WANG Jun | 2010 | Journal of Hydrodynamics2010，22，1： | 11 |
| 10 | Blood perfusion of the contralateral testis evaluated with contrast-enhanced ultrasound in rabbits with unilateral testicular torsion显示文摘The changes of blood perfusion of contralateral testis after unilateral testicular torsion remain controversial.In this study,28 New Zealand white male rabbits were randomly divided into five groups.Group A(n=8),the control group,underwent a sham operation on the unilateral testis without inducing testicular torsion.In groups B,C,and D(n=5 each),unilateral testicular torsion was induced,and,after 3,6 or 24 h,respectively,detorsion was performed.In group E(n=5),permanent unilateral testicular torsion was applied.Contrast-enhanced ultrasound was used to observe the blood perfusion of the contralateral testis at the following stages:pre-torsion(preopration),immediately post-torsion(postopration),pre-detorsion,immediately post-detorsion,and late-stage post-detorsion(6-12 h post-detorsion in groups B-D)or at a similar time point(15-21 h post-torsion in group E).Time-intensity curves were generated,and the following parameters were derived and analyzed:arrival time,time to peak intensity,peak intensity,and half-time of the descending peak intensity.The analysis revealed that blood perfusion of the contralateral testis increased immediately after testicular torsion on the opposite side(P<0.05),which increased with prolonged testicular torsion of the other testis.This research demonstrated that contrast-enhanced ultrasound was valuable in evaluating blood perfusion of the contralateral testis after unilateral testicular torsion. | Lin Chen Wei-Wei Zhan Zhou-Jun Shen Wen-Bin Rui Chen Lv Man Chen Jian-Qiao Zhou Ping Zhou Mi Zhou Ying Zhu | 2009 | Asian Journal of Andrology2009，11，2： | 10 |
| 11 | Characterization and Fine Mapping of a Novel Rice Albino Mutant low temperature albino 1显示文摘白化体异种是为学习叶绿素生合成和叶绿体开发并且克隆在植物涉及这些过程的基因的有用基因资源。这里,我们报导新奇米饭异种低温度白化体 1 (lta1 ) 当在比 20 楢潮低的温度下面成长时,那显示出的白化体在 4 | Yu Peng Yi Zhang Jun Lv Jianhui Zhang Ping Li Xiaoliang Shi Yufeng Wang Honglei Zhang Zuhua He Sheng Teng | 2012 | Journal of Genetics and Genomics2012，39，8： | 9 |
| 12 | Nucleic acid detection in the diagnosis and prevention of schistosomiasis显示文摘Schistosomiasis is an important zoonotic parasitic disease that causes serious harms to humans and animals.Surveillance and diagnosis play key roles in schistosomiasis control,however,current techniques for surveillance and diagnosis of the disease have limitations.As genome data for parasites are increasing,novel techniques for detection incorporating nucleotide sequences are receiving widespread attention.These sensitive,specific,and rapid detection methods are particularly important in the diagnosis of low-grade and early infections,and may prove to have clinical significance.This paper reviews the progress of nucleic acid detection in the diagnosis and prevention of schistosomiasis,including such aspects as the selection of target genes,and development and application of nucleic acid detection methods. | Ping He Lan-gui Song Hui Xie Jin-yi Liang Dong-ya Yuan Zhong-dao Wu Zhi-yue Lv | 2016 | Infectious Diseases of Poverty2016，5，1： | 8 |
| 13 | The effect of dietary tryptophan levels on oxidative stress of liver induced by diquat in weaned piglets显示文摘Oxidative stress can induce abnormal tryptophan metabolism.The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diquat.A total of 36 PIC piglets weaned at 21 days of age were randomly allotted to 1 of 3 diets containing dietary tryptophan levels of 0.18,0.30,and 0.45%for 14 d.On day 8,the piglets were injected intraperitoneally with sterile0.9%NaCI solution or diquat(10 mg/kg body weight).During the first 7 d of trial,increasing dietary tryptophan levels enhanced average daily gain(P=0.09) and average daily feed intake(P=0.08),and decreased the feed efficiency(P<0.05) of piglets.The growth performance was decreased by diquat injection(P<0.05).Diquat injection also decreased the activities of the superoxide dismutase(SOD) and glutathione peroxidase(GPx) in the plasma and liver(P<0.05),increased plasma malondialdehyde(MDA)(P<0.05) and urea nitrogen(P<0.05) concentrations,and enhanced MDA concentration(P =0.09) and tryptophan 2,3-dioxygenase(TDO) activity(P =0.07) in liver of piglets.Increasing dietary tryptophan levels could attenuate the effects of diquat injection on the MDA(P =0.06) concentration and the activities of SOD(P=0.09) and GPx(P=0.05) of the liver,and plasma urea nitrogen(P =0.06) concentration in the piglet.There was a synergistic role for increasing TDO activity in the liver between dietary tryptophan levels and diquat injection(P<0.05).These results suggest that increasing dietary tryptophan levels could attenuate the oxidative stress of the liver in weaned piglets intraperitoneally injected with diquat via enhancing the antioxidant capacity. | Xiangbing Mao Mei Lv Bing Yu Jun He Ping Zheng Jie Yu Quyuan Wang Daiwen Chen | 2015 | Journal of Animal Science and Biotechnology2015，6，1： | 7 |
| 14 | Aeolian transport over a developing transverse dune显示文摘The spatial and temporal changes in aeolian transport over a dune are fundamental factors that control the morphology of the dune.In the present study,we obtained direct field observations of aeolian transport over a developing transverse dune at the Shapotou Aeolian Experiment Site in the southeastern part of China’s Tengger Desert.The transport rate versus wind speed relationship is complicated over a developing dune compared with the relationships over flat surfaces and over dunes that are in equilibrium with the wind.We obtained trend lines for transport rate over the transverse dune versus distance.The transport rate generally increased from the toe to the crest above the stoss slope,but the difference in transport rate between the crest and the toe was smaller than those that have been proposed for taller dunes.The crest/toe ratio for transport rates therefore seems to depend greatly on dune height.Flux density profiles for different points above the dune at different wind speeds were well described by the exponential decay law,as has been proposed for saltation flux density profiles.Coefficients in the flux density profile function can be defined in terms of the transport rate and wind speed.However,the dependence of relative decay rate with height and average saltation height on wind speed was weaker than that observed in a wind tunnel and above a flat surface.The preliminary results obtained in this study require more evidence from field observations to fully describe aeolian transport above developing dunes. | ZhiBao DONG Ping LV ZhengCai ZHANG JunFeng LU | 2014 | Journal of Arid Land2014，6，3： | 7 |
| 15 | Developmental expression of Cyclin H and Cdk7 in zebrafish: the essential role of Cyclin H during early embryo development显示文摘Cyclin 依赖的 kinase (Cdk7 ) 7 是激活 metazoanCdk kinase (CAK ) 的催化子单元。Cdk7 的激活与一个规章的子单元要求它的协会, Cyclin H。尽管 Cdk7/Cyclin H 建筑群在几种在 RNA 的规定被含有,他们在斑马鱼的 orthologs 的精确功能充分没被阐明。在这研究,我们从斑马鱼胞胚胚胎孤立编码人的 Cyclin H 和 Cdk7 的 theorthologs 的二 cDNAs,并且在 zebrafishembryogenesis 检验了 Cdk7/Cyclin H 的角色。顺序分析证明斑马鱼 Cyclin H 和 Cdk7 cDNAs 与 65% 和 86% 身份编码蛋白质到各自的人的 orthologs。RT-PCR 并且在他们在建议的成年的鱼的未受精卵,胚胎和机关的表示的 situhybridization 分析整个山那 Cyclin H 和 Cdk7 消息是装载的似母亲。我们的数据也证明他们的抄本在整个开发被检测。抄本被发现在开发的早阶段期间无所不在并且变得的 Cyclin H 的分发限制了为前面的神经试管,大脑,眼睛,生到 5 天的纸巾,肝和心授精以后。Cyclin H 的 adominant 否定的形式的表示在早胚胎推迟了 zygotic 抄写的发作,导致在授精以后、导致的 5 个小时的 apoptosis 在通常展出 Cyclin H 的高水平的纸巾切断缺点表示。这些结果含有在 transcriptional 机械的规定的 Cyclin H 在中间的胞胚期间转移并且建议它是在早斑马鱼的必要基因幼虫的开发。 | Qing Yun Liu Zhi Li Wu Wen Jian Lv Yuan Chang Yan Yi Ping Li | 2007 | Cell Research2007，17，2： | 7 |
| 16 | Drainage fluid and serum amylase levels accurately predict development of postoperative pancreatic fistula显示文摘AIM To investigate potential biomarkers for predicting postoperative pancreatic fistula( POPF) after pancreaticoduodenectomy(PD).METHODS We prospectively recruited 83 patients to this study. All patients underwent PD(Child's procedure) at the Division of Hepatobiliary and Pancreas Surgery at the First Bethune Hospital of Jilin University between June 2011 and April 2015. Data pertaining to demographic variables, clinical characteristics, texture of pancreas, surgical approach, histopathological results, white blood cell count, amylase and choline levels in the serum, pancreatic/gastric drainage fluid, and choline and amylase levels in abdominal drainage fluid were included in the analysis. Potential correlations between these parameters and postoperative complications such as, POPF, acute pancreatitis, hemorrhage, delayed gastric emptying, and biliary fistula, were assessed. RESULTS Twenty-eight out of the 83(33.7%) patients developed POPF. The severity of POPF was classified as Grade A in 8(28%) patients, grade B in 16(58%), and grade C in4(14%), according to the pancreatic fistula criteria. On univariate and multivariate logistic regression analyses, higher amylase level in the abdominal drainage fluid on postoperative day(POD)1 and higher serum amylase levels on POD4 showed a significant correlation with POPF(P < 0.05). On receiver operating characteristic curve analysis, amylase cut-off level of 2365.5 U/L in the abdominal drainage fluid was associated with a 78.6% sensitivity and 80% specificity [area under the curve(AUC): 0.844; P = 0.009]. A cut-off serum amylase level of 44.2 U/L was associated with a 78.6% sensitivity and 70.9% specificity(AUC: 0.784; P = 0.05).CONCLUSION Amylase level in the abdominal drainage fluid on POD1 and serum amylase level on POD4 represent novel biomarkers associated with POPF development. | Shuo Jin Xiao-Ju Shi Si-Yuan Wang Ping Zhang Guo-Yue Lv Xiao-Hong Du Guang-Yi Wang | 2017 | World Journal of Gastroenterology2017，23，34： | 7 |
| 17 | A critical epitope in CD147 facilitates memory CD4^(+) T-cell hyper-activation in rheumatoid arthritis显示文摘The abnormal activation of CD4^(+)CD45RO+memory T(Tm)cells plays an important role in the pathogenesis of rheumatoid arthritis(RA).Previous studies have shown that CD147 participates in T-cell activation.However,it remains unclear whether CD147 is involved in abnormal Tm-cell activation in RA patients.In this study,we demonstrated that CD147 was predominantly upregulated in Tm cells derived from RA patients.The anti-CD147 mAb 5A12 specifically inhibited Tm-cell activation and proliferation and further restrained osteoclastogenesis.Using a structural–functional approach,we depicted the interface between 5A12 and CD147.This allowed us to identify two critical residues,Lys63 and Asp65,as potential targets for RA treatment,as the double mutation K63A/D65A inhibited Tm-cell activation,mimicking the neutralization by 5A12.This study provides not only a theoretical basis for a“CD147-Tm/Osteoclast-RA chain”for the potential prevention and treatment of RA or other T-cell-mediated autoimmune diseases but also a new target for related drug design and development. | Na Guo Sheng Ye Kui Zhang Xiaoling Yu Hongyong Cui Xiangmin Yang Peng lin Minghua Lv Jinlin Miao Yang Zhang Qing Han Rongguang Zhang Zhinan Chen Ping Zhu | 2019 | Cellular & Molecular Immunology2019，16，6： | 6 |
| 18 | Effect of glial cells on remyelination after spinal cord injury显示文摘Remyelination plays a key role in functional recovery of axons after spinal cord injury.Glial cells are the most abundant cells in the central nervous system.When spinal cord injury occurs,many glial cells at the lesion site are immediately activated,and different cells differentially affect inflammatory reactions after injury.In this review,we aim to discuss the core role of oligodendrocyte precursor cells and crosstalk with the rest of glia and their subcategories in the remyelination process.Activated astrocytes influence proliferation,differentiation,and maturation of oligodendrocyte precursor cells,while activated microglia alter remyelination by regulating the inflammatory reaction after spinal cord injury.Understanding the interaction between oligodendrocyte precursor cells and the rest of glia is necessary when designing a therapeutic plan of remyelination after spinal cord injury. | Hai-feng Wang Xing-kai Liu Rui Li Ping Zhang Ze Chu Chun-li Wang Hua-rui Liu Jun Qi Guo-yue Lv Guang-yi Wang Bin Liu Yan Li Yuan-yi Wang | 2017 | Neural Regeneration Research2017，12，10： | 6 |
| 19 | Pericytes augment glioblastoma cell resistance to temozolomide through CCL5-CCR5 paracrine signaling显示文摘Glioblastoma(GBM)is a prevalent and highly lethal form of glioma,with rapid tumor progression and frequent recurrence.Excessive outgrowth of pericytes in GBM governs the ecology of the perivascular niche,but their function in mediating chemoresistance has not been fully explored.Herein,we uncovered that pericytes potentiate DNA damage repair(DDR)in GBM cells residing in the perivascular niche,which induces temozolomide(TMZ)chemoresistance.We found that increased pericyte proportion correlates with accelerated tumor recurrence and worse prognosis.Genetic depletion of pericytes in GBM xenografts enhances TMZ-induced cytotoxicity and prolongs survival of tumor-bearing mice.Mechanistically,C-C motif chemokine ligand 5(CCL5)secreted by pericytes activates C-C motif chemokine receptor 5(CCR5)on GBM cells to enable DNA-dependent protein kinase catalytic subunit(DNA-PKcs)-mediated DDR upon TMZ treatment.Disrupting CCL5-CCR5 paracrine signaling through the brain-penetrable CCR5 antagonist maraviroc(MVC)potently inhibits pericyte-promoted DDR and effectively improves the chemotherapeutic efficacy of TMZ.GBM patient-derived xenografts with high CCL5 expression benefit from combined treatment with TMZ and MVC.Our study reveals the role of pericytes as an extrinsic stimulator potentiating DDR signaling in GBM cells and suggests that targeting CCL5-CCR5 signaling could be an effective therapeutic strategy to improve chemotherapeutic efficacy against GBM. | Xiao-Ning Zhang Kai-Di Yang Cong Chen Zhi-Cheng He Qiang-Hu Wang Hua Feng Sheng-Qing Lv Yan Wang Min Mao Qing Liu Yao-Yao Tan Wen-Ying Wang Tian-Ran Li Lin-Rong Che Zhong-Yi Qin Ling-Xiang Wu Min Luo Chun-Hua Luo Yu-Qi Liu Wen Yin Chao Wang Hai-Tao Guo Qing-Rui Li Bin Wang Wei Chen Shuang Wang Yu Shi Xiu-Wu Bian Yi-Fang Ping | 2021 | Cell Research2021，31，10： | 5 |
| 20 | Long-term outcomes after radical gastrectomy in gastric cancer patients with overt bleeding显示文摘AIM: To investigate the difference in long-term outcomes between gastric cancer patients with and without a primary symptom of overt bleeding(OB).METHODS: Consecutive patients between January 1, 2007 and March 1, 2012 were identified retrospectively by reviewing a gastric cancer database at Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. A follow-up examination was performed on patients who underwent a radical gastrectomy. OB due to gastric cancer included hematemesis, melena or hematochezia, and gastric cancer was confirmed as the source of bleeding by endoscopy. Patients without OB were defined as cases with occult bleeding and those with other initial presentations, including epigastric pain, weakness, weight loss and obstruction. The 3-year overall survival(OS) rate, age, gender, AJCC T stage, AJCC N stage, overall AJCC stage, tumor size, histological type, macroscopic(Borrmann) type, lymphovascular invasion and R status were compared between patients with and without OB. Moreover, we carried out a subgroup analysis based on tumor location(upper, middle and lower).RESULTS: We identified 939 patients. Of these, 695(74.0%) were hospitalized for potential radical gastrectomy and another 244 received palliative resection, rerouting of the gastrointestinal tract, chemotherapy, radiotherapy or no treatment due to the presence of unresectable tumors. Notably, there was no significant difference in the percentage of OB patients between resectable cases and unresectable cases(20.3 % vs 22.1 %, P = 0.541). Fo l l o w- u p examination was performed on 653 patients(94%) who underwent radical gastrectomy. We found no significant difference in 3-year OS rate(68.2% vs 61.2%, P = 0.143) or clinicopathological characteristics(P > 0.05) between these patients with and without OB. Subgroup analysis based on tumor location showed that the 3-year OS rate of upper gastric cancer was significantly higher in patients with OB(84.6%) than in those without OB(48.1%, P < 0.01) and that AJCC stagesⅠ-Ⅱ(56.4% vs 35.1%, P = 0.017) and T1-T2 category tumors(30.8% vs 13%, P = 0.010) were more frequent in patients with OB than in those without OB. There was no significant difference in 3-year OS rate or clinicopathological characteristics between patients with and without OB(P > 0.05) for middle or lower gastric cancer.CONCLUSION: Upper gastric cancer patients with OB exhibited tumors at less advanced pathological stages and had a better prognosis than upper gastric cancer patients without OB. | Lei Wang Xu-An Wang Jia-Qi Hao Li-Na Zhang Mao-Lan Li Xiang-Song Wu Hao Weng Wen-Jie Lv Wen-Jie Zhang Lei Chen Hong-Gang Xiang Jian-Hua Lu Ying-Bin Liu Ping Dong | 2015 | World Journal of Gastroenterology2015，21，47： | 5 |
您所在的机构暂未开通此部分的功能，请联系管理员