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| 1 | 超高效液相色谱-飞行时间质谱快速分析人参根中的皂甙化合物显示文摘建立了超高效液相色谱-飞行时间质谱快速分析人参根部提取物中的皂甙类化合物的方法。色谱柱为HSST3超高效液相色谱柱(100 mm×2.1 mm,1.8μm);以15 mmol/L甲酸铵水溶液-乙腈为流动相,采用二元梯度洗脱的方式对人参主根的皂甙提取物进行分离。基于待测目标物的多级质谱碎片离子、精确质量等信息,结合9种人参皂甙标准化合物的多级质谱碎片离子质谱图,共鉴定出人参主根提取物中27种皂甙类化合物。在确定的条件下,以9种人参皂甙标样为研究对象,进行了全面的方法学考察,发现它们的线性范围分别为0.33~9.00 mg/L(Rg1),0.11~9.00 mg/L(Re),0.02~2.00 mg/L(Rf),0.07~6.00 mg/L(Rg2),0.04~3.00 mg/L(Rb1,Rb3),0.22~6.00 mg/L(Rc),0.04~9.00 mg/L(Rb2,Rd);在中等加标浓度时,经内标物峰面积校正的9种皂甙标准化合物的峰面积的相对标准偏差(RSD)不高于11.3%;低、中、高3个质量浓度加标水平的回收率范围分别为90%~100%、98%~104%及96%~103%;最低检出限为3.5~18.5μg/L。该方法具有高分辨、快捷、简便、可靠等特点,并成功地应用于分析同一产地、不同生长时间的人参干燥主根中皂甙的差异。可以预计此方法可进一步应用于各种人参原料和制品中皂甙的快速测定。 | 胡春秀 孔宏伟 朱超 魏恒 Thomas HANKEMEIER Jan van der GREEF 王梅 许国旺 | 2011 | 色谱2011，29，6： | 14 |
| 2 | Comparative Transcriptional Profiling and Preliminary Study on Heterosis Mechanism of Super-Hybrid Rice显示文摘杂种优势比也是从二父母表演的后代改善了的生物现象和优异性能生来的父母线。混合米饭是在利用杂种优势的庄稼的最成功的 apotheoses 之一。由基因表示(半自动地面防空系统)的连续分析 F1 超级混血儿的米饭 Liangyou-2186 和它的父母介绍的 Transcriptional 表明 1183 差别表示了基因( DG ),在哪个之中 DG 被发现显著地在象光合作用和碳固定那样的小径充实,并且大多数涉及碳固定小径的关键基因在 F1 混合米饭展出了起来调整的表示。而且,相应的酶的增加的分解代谢的活动和光合的效率也被检测,它联合了显示那碳固定可能在 F1 混血儿,和力量被提高在超级混血儿的米饭与收益活力和杂种优势被联系。由有收益相关的量的特点 loci (QTL ) 的相关 DG,在微分基因表示和 phenotypic 变化之间的一种潜在的关系也被发现。另外,当以前在 Arabidopsis 报导了,包含生理节奏节奏、轻的发信号的小径的一个规章的网络也被发现,它建议如此的一个网络可能也与在混合米饭的杂种优势是相关的。总的来说,现在的学习为理解在米饭位于杂种优势下面的分子的机制提供另一个看法。 | Gui-Sheng Song Hong-Li Zhai Yong-Gang Peng Lei Zhang Gang Wei Xiao- Ying Chen Yu-Guo Xiao Lili Wang Yue-Jun Chen Bin Wu Bin Chen Yu Zhang Hua Chen Xiu-Jing Feng Wan-Kui Gong Yao Liu Zhi-Jie Yin Feng Wang Guo-Zhen Liu Hong-Lin Xu Xiao-Li Wei Xiao-Ling Zhao Pieter B.F. Ouwerkerk Thomas Hankemeier Theo Reijmers Rob van der Heijden Cong-Ming Lu Mei Wang Jan van der Greef Zhen Zhu | 2010 | Molecular Plant2010，3，6： | 12 |
| 3 | Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages显示文摘How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated.Here,we perform single-cell deep-immune profiling of bronchoalveolar lavage(BAL)samples from 5 patients with mild and 26 with critical COVID-19 in comparison to BALs from non-COVID-19 pneumonia and normal lung.We use pseudotime inference to build T-cell and monocyte-to-macrophage trajectories and model gene expression changes along them.In mild COVID-19,CD8^(+)resident-memory(TRM)and CD4^(+)T-helper-17(T_(H17))cells undergo active(presumably antigen-driven)expansion towards the end of the trajectory,and are characterized by good effector functions,while in critical COVID-19 they remain more naïve.Vice versa,CD4^(+)T-cells with T-helper-1 characteristics(TH1-like)and CD8^(+)T-cells expressing exhaustion markers(T_(EX)-like)are enriched halfway their trajectories in mild COVID-19,where they also exhibit good effector functions,while in critical COVID-19 they show evidence of inflammation-associated stress at the end of their trajectories.Monocyte-to-macrophage trajectories show that chronic hyperinflammatory monocytes are enriched in critical COVID-19,while alveolar macrophages,otherwise characterized by anti-inflammatory and antigen-presenting characteristics,are depleted.In critical COVID-19,monocytes contribute to an ATP-purinergic signaling-inflammasome footprint that could enable COVID-19 associated fibrosis and worsen disease-severity.Finally,viral RNA-tracking reveals infected lung epithelial cells,and a significant proportion of neutrophils and macrophages that are involved in viral clearance. | Els Wauters Pierre Van Mol Abhishek Dinkarnath Garg Sander Jansen Yannick Van Herck Lore Vanderbeke Ayse Bassez Bram Boeckx Bert Malengier-Devlies Anna Timmerman Thomas Van Brussel Tina Van Buyten Rogier Schepers Elisabeth Heylen Dieter Dauwe Christophe Dooms Jan Gunst Greet Hermans Philippe Meersseman Dries Testelmans Jonas Yserbyt Sabine Tejpar Walter De Wever Patrick Matthys CONTAGIOUS collaborators Johan Neyts Joost Wauters Junbin Qian Diether Lambrechts | 2021 | Cell Research2021，31，3： | 11 |
| 4 | Different antibiotic susceptibility between antrum and corpus of the stomach,a possible reason for treatment failure of Helicobacter pylori infection显示文摘AIM:To assess whether antibiotic resistance varies between the antrum and corpus of the stomach of patients that are either Helicobacter pylori(H.pylori)therapy-naive or pre-treated.METHODS:H.pylori strains were isolated from antrum and corpus biopsies from 66 patients that received a diagnostic gastroduodenoscopy for variant clinical indications.Antimicrobial susceptibility to amoxicillin,clarithromycin,tetracycline,metronidazole,levofloxacin and rifabutin was tested with the E-test method on IsoSensitest agar with 10 vol%defibrinated horse blood.In patients with a different antibiotic susceptibility pattern between the isolates from the antrum and corpus,DNA fingerprinting via random amplified polymorphic DNA analysis was performed to detect differences among DNA patterns of H.pylori isolates.RESULTS:Primary,secondary and tertiary resistance to clarithromycin was 6.9%,53.8%and 83.3%,retrospectively.Metronidazole and levofloxacin resistance also increased according to the number of previous treatments(17.2%,69.2%,83.3%;13.8%,23.1%,33.3%).Tertiary resistance to rifabutin was detected in12.5%of patients.In none of the 66 patients a resistance against amoxicillin or tetracycline was detectable.Discordant antibiotic susceptibility between antrum and corpus isolates for different antibiotics was seen in 15.2%(10/66)of the patients.Two out of those ten patients were naive to any H.pylori antibiotic treatment.The remaining eight patients previously received at least one eradication therapy.DNA fingerprinting analysis revealed no substantial differences among DNA patterns between antrum and corpus isolates in the majority of patients suggesting an infection with a single H.pylori strain.CONCLUSION:Different antibiotic susceptibility between antrum and corpus biopsies is a common phenomenon and a possible explanation for treatment failure.Resistant H.pylori strains may be missed if just one biopsy from one anatomic site of the stomach is taken for H.pylori susceptibility testing. | Michael Selgrad Ina Tammer Cosima Langner Jan Bornschein Julia Mei?le Arne Kandulski Mariya Varbanova Thomas Wex Dirk Schlüter Peter Malfertheiner | 2014 | World Journal of Gastroenterology2014，20，43： | 10 |
| 5 | Correlation analysis of dual-energy CT iodine maps with quantitative pulmonary perfusion MRI显示文摘AIM:To correlate dual-energy computed tomography(DECT) pulmonary angiography derived iodine maps with parameter maps of quantitative pulmonary perfusion magnetic resonance imaging(MRI).METHODS:Eighteen patients with pulmonary perfusion defects detected on DECT derived iodine maps were included in this prospective study and additionally underwent time-resolved contrast-enhanced pulmonary MRI [dynamic contrast enhanced(DCE)-MRI].DCE-MRI data were quantitatively analyzed using a pixel-by-pixel deconvolution analysis calculating regional pulmonary blood flow(PBF),pulmonary blood volume(PBV) and mean transit time(MTT) in visually normal lung parenchyma and perfusion defects.Perfusion parameterswere correlated to mean attenuation values of normal lung and perfusion defects on DECT iodine maps.Two readers rated the concordance of perfusion defects in a visual analysis using a 5-point Likert-scale(1 = no correlation,5 = excellent correlation).RESULTS:In visually normal pulmonary tissue mean DECT and MRI values were:22.6 ± 8.3 Hounsfield units(HU);PBF:58.8 ± 36.0 mL/100 mL per minute;PBV:16.6 ± 8.5 mL;MTT:17.1 ± 10.3 s.In areas with restricted perfusion mean DECT and MRI values were:4.0 ± 3.9 HU;PBF:10.3 ± 5.5 mL/100 mL per minute,PBV:5 ± 4 mL,MTT:21.6 ± 14.0 s.The differences between visually normal parenchyma and areas of restricted perfusion were statistically significant for PBF,PBV and DECT(P < 0.0001).No linear correlation was found between MRI perfusion parameters and attenuation values of DECT iodine maps(PBF:r = 0.35,P = 0.15;PBV:r = 0.34,P = 0.16;MTT:r = 0.41,P = 0.08).Visual analysis revealed a moderate correlation between perfusion defects on DECT iodine maps and the parameter maps of DCE-MRI(mean score 3.6,k 0.45).CONCLUSION:There is a moderate visual but not statistically significant correlation between DECT iodine maps and perfusion parameter maps of DCE-MRI. | Jan Hansmann Paul Apfaltrer Frank G Zoellner Thomas Henzler Mathias Meyer Gerald Weisser Stefan O Schoenberg Ulrike I Attenberger | 2013 | World Journal of Radiology2013，5，5： | 6 |
| 6 | PEPT study of particle motion for different riser exit geometries显示文摘Laboratory and industrial risers are equipped with exits of many different layouts,and numerous publications discuss the influence of riser exit geometry on local and overall solids hydrodynamics in the riser.The present paper reviews literature findings-mostly based upon indirect experimental techniques and often somewhat contradictory.Direct measurement of particle velocity and particle occupancy near and in the riser exit provide a better indication of the effect of riser exit geometry.Positron Emission Particle Tracking(PEPT)was used in this work for the first time to investigate the exit region of the riser.An abrupt or sharp exit causes particles to be knocked out of the gas flow,so forming a recirculation or reflux region in the upper part of the riser.This is much less pronounced with a curved or gradual exit. | Chian Wen Chan Anke Brems Shiva Mahmoudi Jan Baeyens Jonathan Seville David Parker Thomas Leadbeater Joseph Gargiuli | 2010 | Particuology2010，8，6： | 5 |
| 7 | Stromal Elements Act to Restrain, Rather Than Support, Pancreatic Ductal Adenocarcinoma显示文摘 | Andrew D. Rhim Paul E. Oberstein Dafydd H. Thomas Emily T. Mirek Carmine F. Palermo Stephen A. Sastra Erin N. Dekleva Tyler Saunders Claudia P. Becerra Ian W. Tattersall C. Benedikt Westphalen Jan Kitajewski Maite G. Fernandez-Barrena Martin E. Fernandez- | 2014 | Cancer Cell2014，，： | 5 |
| 8 | HPLC/LTQ-FTMS用于白参中的丙二酰基人参皂苷的研究显示文摘在15mM甲酸铵作为流动相添加剂条件下,采用HPLC/LTQ-FTMS实现了白参样品中酸性人参皂苷的优化分离。根据高分辨质谱、多级质谱信息,结合保留规律验证,在白参萃取液中共检测和识别了八种丙二酰基人参皂苷,包括丙二酰基人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rb3和Rd。 | 孔宏伟 王梅 Rob van der Heijden Jan van der Greef Thomas Hankemeier 许国旺 | 2009 | 世界科学技术-中医药现代化2009，11，1： | 5 |
| 9 | Debris Flows Risk Analysis and Direct Loss Estimation:the Case Study of Valtellina di Tirano,Italy显示文摘Landslide risk analysis is one of the primary studies providing essential instructions to the subsequent risk management process. The quantification of tangible and intangible potential losses is a critical step because it provides essential data upon which judgments can be made and policy can be formulated. This study aims at quantifying direct economic losses from debris flows at a medium scale in the study area in Italian Central Alps. Available hazard maps were the main inputs of this study. These maps were overlaid with information concerning elements at risk and their economic value. Then, a combination of both market and construction values was used to obtain estimates of future economic losses. As a result, two direct economic risk maps were prepared together with risk curves, useful to summarize expected monetary damage against the respective hazard probability. Afterwards, a qualitative risk map derived using a risk matrix officially provided by the set of laws issued by the regional government, was prepared. The results delimit areas of high economic as well as strategic importance which might be affected by debris flows in the future. Aside from limitations and inaccuracies inherently included in risk analysis process, identification of high risk areas allows local authorities to focus their attention on the 'hot-spots', where important consequences may arise and local(large) scale analysis needs to be performed with more precise cost-effectiveness ratio. The risk maps can be also used by the local authorities to increase population's adaptive capacity in the disaster prevention process. | Jan BLAHUT Thomas GLADE Simone STERLACCHINI | 2014 | Journal of Mountain Science2014，11，2： | 5 |
| 10 | RANK-ligand and osteoprotegerin as biomarkers in the differentiation between periprosthetic joint infection and aseptic prosthesis loosening显示文摘AIM To assess serum levels of RANK-ligand(RANKL) and osteoprotegerin(OPG) as biomarkers for periprosthetic joint infection(PJI) and compare their accuracy with standard tests.METHODS One hundred and twenty patients presenting with a painful total knee or hip arthroplasty with indication for surgical revision were included in this prospective clinical trial. Based on standard diagnostics(joint aspirate, microbiological, and histological samples) and Musculoskeletal Infection Society consensus classification,patients were categorized into PJI, aseptic loosening,and control groups. Implant loosening was assessed radiographically and intraoperatively. Preoperative serum samples were collected and analyzed for RANKL, OPG, calcium, phosphate, alkaline phosphatase(AP), and the bone-specific subform of AP(b AP). Statistical analysis was carried out, testing for significant differences between the three groups and between stable and loose implants. RESULTS All three groups were identical in regards to age, gender, and joint distribution. No statistically significant differences in the serum concentration of RANKL(P = 0.16) and OPG(P = 0.45) were found between aseptic loosening and PJI, with a trend towards lower RANKL concentrations and higher OPG concentrations in the PJI group. The RANKL/OPG ratio was significant for the comparison between PJI and non-PJI(P = 0.005). A ratio > 60 ruled out PJI in all cases(specificity: 100%, 95%CI: 89, 11% to 100.0%) but only 30% of non-PJI patients crossed this threshold. The positive predictive value remained poor at any cut-off. In the differentiation between stable and loose implants, none of the parameters measured(calcium, phosphate, AP, and b AP) showed a significant difference, and only AP and b AP measurements showed a tendency towards higher values in the loosened group(with P = 0.09 for AP and P = 0.19 for b AP). CONCLUSION Lower RANKL and higher OPG concentrations could be detected in PJI, without statistical significance. | Max J Friedrich Matthias D Wimmer Jan Schmolders Andreas C Strauss Milena M Ploeger Hendrik Kohlhof Dieter C Wirtz Sascha Gravius Thomas M Randau | 2017 | World Journal of Orthopedics2017，8，4： | 4 |
| 11 | Adenoma detection with cap-assisted colonoscopy versus regular colonoscopy: a randomised controlled trial显示文摘 | Thomas R de Wijkerslooth Esther M Stoop Patrick M Bossuyt Elisabeth M H Mathus-Vliegen Jan Dees Kristien M A J Tytgat Monique E van Leerdam Paul Fockens Ernst J Kuipers Evelien Dekker | 2012 | Gut2012，，10： | 3 |
| 12 | MicroRNAs in the Human Heart: A Clue to Fetal Gene Reprogramming in Heart Failure显示文摘 | Thomas Thum Paolo Galuppo Christian Wolf Jan Fiedler Susanne Kneitz Linda W. van Laake Pieter A. Doevendans Christine L. Mummery Jürgen Borlak Axel Haverich Carina Gross Stefan Engelhardt Georg Ertl Johann Bauersachs | 2007 | Circulation2007，，3： | 3 |
| 13 | Tumour response and secondary resectability of colorectal liver metastases following neoadjuvant chemotherapy with cetuximab: the CELIM randomised phase 2 trial显示文摘 | Gunnar Folprecht Thomas Gruenberger Wolf O Bechstein Hans-Rudolf Raab Florian Lordick J?rg T Hartmann Hauke Lang Andrea Frilling Jan Stoehlmacher Jürgen Weitz Ralf Konopke Christian Stroszczynski Torsten Liersch Detlev Ockert Thomas Herrmann Eray Goekkurt | 2010 | Lancet Oncology2010，，1： | 3 |
| 14 | 天津地区住宅热舒适和建筑空调使用行为研究显示文摘通过对天津地区41户家庭室内空气干球温度、相对湿度等热环境参数6个月的长期测试,研究了住宅室内热环境和居民空调使用行为习惯。通过电子问卷调查了居民热感觉和热舒适。采用统计学分析方法得到天津地区住宅热中性温度为24.7℃,80%可接受温度区间为21.0~27.3℃。天津地区家庭人员耐热性要好于办公建筑人员。同时将室内热中性温度和室外空气干球温度联系起来,建立了天津地区住宅适应性热舒适模型。 | 宋阳瑞 孙越霞 罗述刚 田喆 侯静 Jan Sundell Kim Jungsoo Parkinson Thomas de Dear Richard | 2018 | 暖通空调2018，48，10： | 3 |
| 15 | The constituent equation of piezoelectric bi- morphs显示文摘 | SMITS Jan G DALKE Susan I COONEY Thomas K | 1991 | Sensors and Actuator A1991，28，1： | 2 |
| 16 | Allocating ecological footprints to final consumption categories with input–output analysis显示文摘 | Thomas Wiedmann Jan Minx John Barrett Mathis Wackernagel | 2005 | Ecological Economics2005，，1： | 2 |
| 17 | 智能数字化的全流程工艺优化方案显示文摘介绍了普锐特冶金技术的全流程工艺优化方案,通过开发工艺诀窍,优化工艺质量控制系统,可使整个钢铁生产链实现不同工艺设备间的智能化、数字化互联;提高工艺稳定性、增大工艺灵活性,提高生产效率;生产优质产品,满足客户要求。 | Jan Friedemann Plaul Wolfgang Oberaigner 翟玉友 Thomas Pfatschbacher Manfred Kuegel | 2019 | 河北冶金2019，，4： | 2 |
| 18 | Parameters of a severe disease course in ulcerative colitis显示文摘AIM:To detect high risk patients with a progressive disease course of ulcerative colitis(UC) requiring immunosuppressive therapy(IT).METHODS:A retrospective,multicenter analysis of 262 UC patients from eight German tertiary inflammatory bowel disease centres was performed.Patients were divided into two groups depending on the patients need to initiate immunosuppressive therapy in the disease course.A comparison between the two groups was made with regard to demographics,clinical and laboratory parameters obtained within three months after UC diagnosis and the response to first medical therapy.Using this data,a prognostic model was established to predict the individual patients probability of requiring an immunosuppressive therapy.RESULTS:In 104(39.7%) out of 262 patients,UC therapy required an immunosuppressive treatment.Patients in this group were significantly younger at time of diagnosis(HR = 0.981 ± 0.014 per year,P = 0.009),and required significantly more often a hospitalisation(HR = 2.5 ± 1.0,P < 0.001) and a systemic corticosteroid therapy at disease onset(HR = 2.4 ± 0.8,P < 0.001),respectively.Response to steroid treatment was significantly different between the two groups of patients(HR = 5.2 ± 3.9 to 50.8 ± 35.6 compared to no steroids,P = 0.016 to P < 0.001).Furthermore,in the IT group an extended disease(HR = 3.5 ± 2.4 to 6.1 ± 4.0 compared to proctitis,P = 0.007 to P = 0.001),anemia(HR = 2.2 ± 0.8,P < 0.001),thrombocytosis(HR = 1.9 ± 1.8,P = 0.009),elevated C-reactive protein(CRP)(HR = 2.1 ± 0.9,P < 0.001),and extraintestinal manifestations in the course of disease(HR = 2.6 ± 1.1,P = 0.004) were observed.Six simple clinical items were used to establish a prognostic model to predict the individual risk requiring an IT.This probability ranges from less than 2% up to 100% after 5 years.Using this,the necessity of an immunosuppressive therapy can be predicted in 60% of patients.Our model can determine the need for an immunosuppressive drug therapy or if a 'watch and wait' approach is reasonable already early in the treatment course of UC.CONCLUSION:Using six simple clinical parameters,we can estimate the patients individual risk of developing a progressive disease course. | Andreas Stallmach Luisa Nickel Thomas Lehmann Bernd Bokemeyer Martin Bürger Dietrich Hüppe Wolfgang Kruis Susanna Nikolaus Jan C Preiss Andreas Sturm Niels Teich Carsten Schmidt | 2014 | World Journal of Gastroenterology2014，20，35： | 2 |
| 19 | In the name of the rose:a roadmap for rose research in the genome era显示文摘The recent completion of the rose genome sequence is not the end of a process,but rather a starting point that opens up a whole set of new and exciting activities.Next to a high-quality genome sequence other genomic tools have also become available for rose,including transcriptomics data,a high-density single-nucleotide polymorphism array and software to perform linkage and quantitative trait locus mapping in polyploids.Rose cultivars are highly heterogeneous and diverse.This vast diversity in cultivated roses can be explained through the genetic potential of the genus,introgressions from wild species into commercial tetraploid germplasm and the inimitable efforts of historical breeders.We can now investigate how this diversity can best be exploited and refined in future breeding work,given the rich molecular toolbox now available to the rose breeding community.This paper presents possible lines of research now that rose has entered the genomics era,and attempts to partially answer the question that arises after the completion of any draft genome sequence:‘Now that we have“the”genome,what’s next?’.Having access to a genome sequence will allow both(fundamental)scientific and(applied)breeding-orientated questions to be addressed.We outline possible approaches for a number of these questions. | Marinus J.M.Smulders Paul Arens Peter M.Bourke Thomas Debener Marcus Linde Jan De Riek Leen Leus Tom Ruttink Sylvie Baudino Laurence Hibrant Saint-Oyant Jeremy Clotault Fabrice Foucher | 2019 | Horticulture Research2019，6，1： | 2 |
| 20 | CREMa overexpression decreases IL-2 production,induces a TH17 phenotype and accelerates autoimmunity显示文摘Dear Editor,External cytokines produced by cells of the innate immune system induce the dif-ferentiation of CD4+T cells into helper T cell subsets with distinct functions and cytokine profiles.Apart from TH1 and TH2 cells a third subset of helper T cells has been described,which is characterized by increased expression of IL-17A,IL-17F,IL-21 and IL-22(TH17 cells). | Ralph Lippe Kim Ohl Georg Varga Thomas Rauen Jose C.Crispin Yuang-Taung Juang Stefanie Kuerten Frank Tacke Marc Wolf Kirsten Roebrock Thomas Vogl Eva Verjans Nora Honke Jan Ehrchen Dirk Foell Boris Skryabin Norbert Wagner George C.Tsokos Johannes Roth Klaus Tenbrock | 2012 | Journal of Molecular Cell Biology2012，4，2： | 2 |
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