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| 1 | Beta-7 integrin controls enterocyte migration in the small intestine显示文摘AIM:To hypothesize that beta-7 integrin affects cellularmigration of both,lymphocytes and enterocytes.METHODS:The nucleoside analog Brd U was ip injected in beta-7-deficient mice(C57BL/6-Itgbtmlcgn/J)of male gender and age-matched male C57BL/J J mice(wild type)4,20,or 40 h before analysis.The total small intestine was isolated,dissected,and used for morphometrical studies.Brd U-positive epithelial cells were numbered in at least 15 hemi-crypts per duodenum,jejunum,and ileum of each animal.The outer most Brd U-positive cell(cellmax)was determined per hemi-crypt,numerically documented,and statistically analysed.RESULTS:Integrins containing the beta-7-chain were exclusively expressed on leukocytes.In the small intestinal mucosa of beta-7 integrin-deficient mice the number of intraepithelial lymphocytes was drastically decreased.Moreover,the Peyer’s patches of beta-7integrin-deficient mice appeared hypoplastic.In beta-7integrin-deficient mice the location of cellmax was found in a higher position than it was the case for the controls.The difference was already detected at 4 h after Brd U application,but significantly increased with time(40 h after Brd U injection)in all small intestinal segments investigated,i.e.,duodenum,jejunum,and ileum.Migration of small intestinal enterocytes was different between the experimental groups measured by cellmax locations.CONCLUSION:The E-cadherin beta-7 integrin pathway probably controls migration of enterocytes within the small intestinal surface lining epithelial layer. | Elke Kaemmerer Paula Kuhn Ursula Schneider Thomas Clahsen Min Kyung Jeon Christina Klaus Julia Andruszkow Michael Hrer Sabine Ernst Angela Schippers Norbert Wagner Nikolaus Gassler | 2015 | World Journal of Gastroenterology2015，21，6： | 3 |
| 2 | CREMa overexpression decreases IL-2 production,induces a TH17 phenotype and accelerates autoimmunity显示文摘Dear Editor,External cytokines produced by cells of the innate immune system induce the dif-ferentiation of CD4+T cells into helper T cell subsets with distinct functions and cytokine profiles.Apart from TH1 and TH2 cells a third subset of helper T cells has been described,which is characterized by increased expression of IL-17A,IL-17F,IL-21 and IL-22(TH17 cells). | Ralph Lippe Kim Ohl Georg Varga Thomas Rauen Jose C.Crispin Yuang-Taung Juang Stefanie Kuerten Frank Tacke Marc Wolf Kirsten Roebrock Thomas Vogl Eva Verjans Nora Honke Jan Ehrchen Dirk Foell Boris Skryabin Norbert Wagner George C.Tsokos Johannes Roth Klaus Tenbrock | 2012 | Journal of Molecular Cell Biology2012，4，2： | 2 |
| 3 | Gastrectomy with isoperistaltic jejunal parallel pouch in a 15-year-old adolescent boy with gastric adenocarcinoma and autosomal recessive agammaglobulinemia显示文摘 | Jan E. Slotta Sabine Heine Anne Kauffels Thomas Krenn Frank Grünhage Mathias Wagner Norbert Graf Martin K. Schilling Jochen Schuld | 2011 | Journal of Pediatric Surgery2011，，9： | 1 |
| 4 | Critical role forβ7 integrins in formation of the gut-associated lymphoid tissue显示文摘 | Norbert Wagner Jurgen Lohler Kunkel EJ | 1996 | Nature1996，382，6589： | 1 |
| 5 | Impaired sleep quality and sleep duration in smokers—results from the G erman M ulticenter S tudy on N icotine D ependence显示文摘 | Stefan Cohrs Andrea Rodenbeck Dieter Riemann Bertram Szagun Andreas Jaehne Jürgen Brinkmeyer Gerhard Gründer Thomas Wienker Amalia Diaz‐Lacava Arian Mobascher Norbert Dahmen Norbert Thuerauf Johannes Kornhuber Falk Kiefer Jürgen Gallinat Michael Wagner Di | 2014 | Addiction Biology2014，，3： | 1 |
| 6 | Intestinal Tolerance Requires Gut Homing and Expansion of FoxP3 + Regulatory T Cells in the Lamina Propria显示文摘 | Usriansyah Hadis Benjamin Wahl Olga Schulz Matthias Hardtke-Wolenski Angela Schippers Norbert Wagner Werner Müller Tim Sparwasser Reinhold F?rster Oliver Pabst | 2011 | Immunity2011，，2： | 1 |
| 7 | Intestinal Tolerance Requires Gut Homing and Expansion of FoxP3 + Regulatory T Cells in the Lamina Propria显示文摘 | Usriansyah Hadis Benjamin Wahl Olga Schulz Matthias Hardtke-Wolenski Angela Schippers Norbert Wagner Werner Müller Tim Sparwasser Reinhold F?rster Oliver Pabst | 2011 | Immunity2011，，2： | 1 |
| 8 | Is there a role of the Thr164Ile-β2-adrenoceptor polymorphism for the outcome of chronic heart failure?显示文摘 | Kirsten Leineweber Gero Tenderich Christina Wolf S?ren Wagner Armin Zittermann Miriam Elter-Schulz Reiner Moog Norbert Müller Heinz-Günther Jakob Reiner K?rfer Thomas Philipp Gerd Heusch Otto-Erich Brodde PhD | 2006 | Basic Research in Cardiology2006，，6： | 1 |
| 9 | Intestinal genetic inactivation of caspase-8 diminishes migration of enterocytes显示文摘AIM: To verify the hypothesis that caspase-8(Casp8), which regulates cellular apoptosis and necroptosis, is critically involved in enterocyte migration.METHODS: Casp8-silenced Caco2 cells were used in migration assays. In addition, enterocyte-specific Casp8 heterozygous(Casp8+/?int) or homozygous knockout mice(Casp8?int) were generated by crossing genetically modified mice carrying lox P recombination sites in intron 2 and 4 of the murine Casp8 gene with transgenic animals expressing a cre-transgene under control of the villin promoter in a pure C57/BL6 genetic background. The nucleoside analog Brd U was injected i.p. in male Casp8+/?int and Casp8?int animals 4 h, 20 h, or 40 h before performing morphometric studies. Locations of anti-Brd U-immunostained cells(cellmax) in at least 50 hemi-crypts of 6 histoanatomically distinct intestinal mucosal regions were numbered and extracted for statistical procedures. For the mice cohort(n = 28), the walking distance of enterocytes was evaluated from cellmax within crypt(n = 57), plateau(n = 19), and villus(n = 172) positions, resulting in a total of 6838 observations. Data analysis was performed by fitting a three-level mixed effects modelto the data.RESULTS: In cell culture experiments with Caco2 cells, Casp8 knockdown efficiency mediated by RNA interference on Casp8 transcripts was 80% controlled as determined by Western blotting. In the scratch assay, migration of Casp8-deleted Caco2 cells was significantly diminished when compared with controls(Casp8?scramble and Caco2). In Brd U-labeled Casp8?int mice, cellmax locations were found along the hemi-crypts in a lower position than it was for Casp8+/?int or control(cre-negative) animals. Statistical data analysis with a three-level mixed effects model revealed that in the six different intestinal locations(distinct segments of the small and large intestine), cell movement between the three mice groups differed widely. Especially in duodenal hemi-crypts, enterocyte movement was different between the groups. At 20 h, duodenal cellmax location was significantly lower in Casp8?int(25.67 ± 2.49) than in Casp8+/?int(35.67 ± 4.78; P < 0.05) or control littermates(44.33 ± 0.94; P < 0.01).CONCLUSION: C a s p 8- d e p e n d e n t m i g ra t i o n o f enterocytes is likely involved in intestinal physiology and inflammation-related pathophysiology. | Elke Kaemmerer Paula Kuhn Ursula Schneider Min Kyung Jeon Christina Klaus Miriam Schiffer Danika Weisner Christian Liedtke J?rg J?kel Lieven Nils Kennes Ralf-Dieter Hilgers Norbert Wagner Nikolaus Gassler | 2015 | World Journal of Gastroenterology2015，21，15： | 1 |
| 10 | Degradation study on tin- and bismuth-based gas-diffusion electrodes during electrochemical CO_(2) reduction in highly alkaline media显示文摘This work investigated the degradation of tin – based gas-diffusion electrodes (GDE) and also a promising Bi2O3 GDE in electrochemical CO_(2) reduction in highly alkaline media which has not been studied before. The contributions of the electrode wetting (or flooding, if excessively) and catalyst leaching on the degradation were analyzed. Therefore, electrochemical impedance spectroscopy was used to monitor the wetted surface area of the GDE in combination with post-mortem analysis of the penetration depth by visualizing the electrolyte’s cation in the GDE cross-section. Furthermore, to reveal a possible degradation of the electrocatalyst, its distribution was mapped in the GDEs cross-section after operation while the catholyte was additionally analyzed via ICP-MS. The results clearly demonstrate that the SnO_(2) catalyst dissolves in the reaction zone inside the GDE and might be partially redeposited near the GDEs surface. Since the redeposition process occurs only partially a steady loss of catalyst was observed impeding a clear distinction of the two degradation phenomena. Nevertheless, the deterioration of the electrode performance measured as faraday efficiency (FE) of the parasitic hydrogen evolution reaction (HER) qualitatively correlates with the differential double layer capacitance (Cdl). A significant difference of the rate of increase for the hydrogen FE and Cdl can be ascribed to the superposition of both above-mentioned degradation mechanisms. The demonstrated instability of SnO_(2) contrasts with the behavior of Bi2O3 GDE which is stabilized during CO_(2) conversion by redeposition of the diluted dissolved species as metallic Bi which is active for the CO_(2) reduction reaction. | Fabian Bienen Armin Lowe Joachim Hildebrand Sebastian Hertle Dana Schonvogel Dennis Kopljar Norbert Wagner Elias Klemm Kaspar Andreas Friedrich | 2021 | Journal of Energy Chemistry2021，30，11： | 0 |
| 11 | Factors influencing communication of traditional Chinese medicine use between patients and doctors:A multisite cross-sectional study显示文摘Objective: In Singapore, the use of traditional Chinese medicine(TCM) alongside Western medicine(WM)is common. There are risks of adverse herb-drug interactions when taken concurrently. Current literature suggests that TCM use is not regularly reported to WM doctors in Singapore, but the underlying reasons are not understood.Methods: A cross-sectional study was conducted across Singapore by administering questionnaires to TCM-using patients and WM-practising general practitioners(GPs). The questionnaire examined the following themes:(1) demographics and TCM use pattern;(2) respondents'(patients and GPs) knowledge and beliefs about TCM and the factors influencing the discussion of TCM during the WM consultation;and(3) respondents' qualitative suggestions to increase disclosure rate.Results: A total of 484 patients and 334 GPs were surveyed. Factors associated with patients' initiation of TCM discussion include length of consultation(odds ratio [OR]: 2.1;P < 0.001), comfort level in discussing TCM(OR: 1.6;P < 0.001) and belief in importance of discussion(OR: 1.4;P = 0.017). Doctor's initiation of discussion(74%) was the top patient-ranked factor influencing their discussion of TCM. For doctors,knowledge of TCM indications(OR: 2.2;P < 0.001), belief in importance of discussion(OR: 2.1;P < 0.001) and comfort level in discussing TCM(OR: 1.9;P = 0.001) were associated with their initiation of TCM use discussion. Possible WM-TCM interactions(58%) was the top doctor-ranked factor influencing their discussion of TCM.Conclusion: The discussion of TCM in a WM setting is multifactorial. Interventions include doctors' active screening for TCM use in patients and equipping doctors with TCM knowledge. Improving communication between patients and doctors is key to avoiding harmful herb-drug interactions. | Wen Qiang Lee Jeremy Teoh Pei Zheng Kenneth Lee Zhi Xiong Gerard Low Xueling Sim Foong Fong Mary Chong Norbert Ludwig Wagner | 2019 | Journal of Integrative Medicine2019，17，6： | 0 |
| 12 | Uptake of citrate-coated iron oxide nanoparticles into atherosclerotic lesions in mice occurs via accelerated transcytosis through plaque endothelial cells显示文摘很小的 superparamagnetic 氧化铁 nanoparticles (VSOP ) 很快在动脉粥样硬化患者损害积累,由磁性的回声成像(MRI ) 的从而创新的匾可视化。这研究被执行识别 VSOP 的举起机制进动脉粥样硬化患者匾。低密度的脂蛋白受体缺乏(有先进动脉粥样硬化的 LDLR /) 老鼠在 VSOP 的静脉内的管理以后在各种各样的时间点用 MRI 和传播电子显微镜学(TEM ) 被分析。验尸, MRI 检测了在注射以后动脉粥样硬化患者匾把 10 min 标记的 VSOP,并且信号在开始的 3 h 上增加了。TEM 表明标记的集中的匾被 VSOP 的加速的 transcytosis 通过覆盖动脉粥样硬化患者损害的 endothelial 房间调停。有 endocytosis 禁止者和小介入 RNA (siRNA ) 的实验揭示了包含调停 clathrin 、调停 caveolin 的过程的 dynamin 依赖的机制。在房间文化实验, endothelial VSOP 举起在 proatherogenic 流动和 TNF 刺激下面被提高,两个都是在匾区域的现在的条件。我们的学习证明 VSOP 启用对加速的 endothelial transcytosis 的非侵略的 MRI 评价,在动脉粥样硬化患者的重要 pathomechanism 匾形成。 | Wolfram C. Poller Evelyn Ramberger Philipp Boehm-Sturm Susanne Mueller Konstantin Moller Norbert Lowa Frank Wiekhorst Susanne Wagner Matthias Taupitz Eyk Schellenberger Gert Baumann Karl Stangl Verena Stangl Antje Ludwig | 2016 | Nano Research2016，9，11： | 0 |
| 13 | Fatty acid binding receptors in intestinal physiology and pathophysiology显示文摘Free fatty acids are essential dietary components and recognized as important molecules in the maintenance of cellular homeostasis.In the last decade,the molecular pathways for free fatty acid sensing in the gastrointestinal tract have been further elucidated by molecular identification and functional characterization of fatty acid binding receptors.These sensing molecules belong to the family of G proteincoupled receptors.In the intestine,four important receptors have been described so far.They differ in molecular structure,ligand specificity,expression pattern,and functional properties.In this review,an overview of intestinal fatty acid binding receptors and their role in intestinal physiology and pathophysiology is given. | Elke Kaemmerer Patrick Plum Christina Klaus Ralf Weiskirchen Christian Liedtke Maximilian Adolf Angela Schippers Norbert Wagner Andrea Reinartz Nikolaus Gassler | 2010 | World Journal of Gastrointestinal Pathophysiology2010，1，5： | 0 |
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