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| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | Relationship between depression and diabetes in pregnancy: A systematic review显示文摘AIM To systematically review the literature on women with both diabetes in pregnancy(DIP) and depression during or after pregnancy. METHODS In this systematic literature review, PubM ed/MEDLINE and EMBASE were searched(13 November 2015) using terms for diabetes(type 1, type 2, or gestational), depression, and pregnancy(no language or date restrictions). Publications that reported on women who had both DIP(any type) and depression or depressive symptoms before, during, or within one year after pregnancy were considered for inclusion. All study types were eligible for inclusion; conference abstracts, narrative reviews, nonclinical letters, editorials, and commentaries were excluded, unless they provided treatment guidance.RESULTS Of 1189 articles identified, 48 articles describing women with both DIP and depression were included(sample sizes 36 to > 32 million). Overall study quality was poor; most studies were observational, and only 12 studies(mostly retrospective database studies) required clinical depression diagnosis. The prevalence of concurrent DIP(any type) and depression in general populations of pregnant women ranged from 0% to 1.6%(median 0.61%; 12 studies). The prevalence of depression among women with gestational diabetes ranged from 4.1% to 80%(median 14.7%; 16 studies). Many studies examined whether DIP was a risk factor for depression or depression was a risk factor for DIP. However, there was no clear consensus for either relationship. Importantly, we found limited guidance on the management of women with both DIP and depression. CONCLUSION Given the increasing prevalence of diabetes and depression, high-quality research and specific guidance for management of pregnant women with both conditions are warranted. | Glynis P Ross Henrik Falhammar Roger Chen Helen Barraclough Ole Kleivenes Ian Gallen | 2016 | World Journal of Diabetes2016，7，19： | 3 |
| 3 | Causes of encephalitis and differences in their clinical presentations in England: a multicentre, population-based prospective study显示文摘 | Julia Granerod Helen E Ambrose Nicholas WS Davies Jonathan P Clewley Amanda L Walsh Dilys Morgan Richard Cunningham Mark Zuckerman Ken J Mutton Tom Solomon Katherine N Ward Michael PT Lunn Sarosh R Irani Angela Vincent David WG Brown Natasha S Crowcroft | 2010 | The Lancet Infectious Diseases2010，，12： | 2 |
| 4 | Exogenous cytokinin application to Actinidia chinensis var. deliciosa ‘Hayward’ fruit promotes fruit expansion through water uptake显示文摘Exogenous application of a cytokinin-like compound forchlorfenuron(CPPU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit quality.Actinidia chinensis var.deliciosa‘Hayward’fruit are very responsive to CPPU treatments,but the mechanism underlying the significant fruit weight increase and associated decrease in DM is unclear.In this study,we hypothesised that CPPU-enhanced growth increases fruit carbohydrate demand,but limited carbohydrate supply resulted in decreased fruit DM.During fruit development,CPPU effects on physical parameters,metabolites,osmotic pressure and transcriptional changes were assessed under conditions of both standard and a high carbohydrate supply.We showed that CPPU increased fruit fresh weight but the dramatic DM decrease was not carbohydrate limited.Enhanced glucose and fructose concentrations contributed to an increase in soluble carbohydrate osmotic pressure,which was correlated with increased water accumulation in CPPU-treated fruit and up-regulation of water channel aquaporin gene PIP2.4 at 49 days after anthesis.Transcipt analysis suggested that the molecular mechanism contributing to increased glucose and fructose concentrations was altered by carbohydrate supply.At standard carbohydrate supply,the early glucose increase in CPPU fruit was associated with reduced starch synthesis and increased starch degradation.When carbohydrate supply was high,the early glucose increase in CPPU fruit was associated with a general decrease in starch synthesis but up-regulation of vacuolar invertase and fructokinase genes.We conclude that CPPU affected fruit expansion by increasing the osmotically-driven water uptake and its effect was not carbohydrate supply-limited. | Simona Nardozza Helen L Boldingh Mark W Wohlers Andrew P Gleave Zhiwei Luo Guglielmo Costa Elspeth A MacRae Michael J Clearwater Annette C Richardson | 2017 | Horticulture Research2017，4，1： | 2 |
| 5 | Adsorption of copper (Ⅱ) and chromium (Ⅲ) ions onto amidoximated cellulose 显示文摘 | RIMA S HELENE G MICHELE P R | 2000 | Journal of Applied Polymer Science2000，75，13： | 1 |
| 6 | CD28 costimulation improves expansion and persistence of chimeric antigen receptor-modified T cells in lymphoma patients显示文摘 | Savoldo Barbara Ramos Carlos Almeida Liu Enli Mims Martha P Keating Michael J Carrum George Kamble Rammurti T Bollard Catherine M Gee Adrian P Mei Zhuyong Liu Hao Grilley Bambi Rooney Cliona M Heslop Helen E Brenner Malcolm K Dotti Gianpie | 2011 | Journal of Clinical Investigation2011，，5： | 1 |
| 7 | Protein expression by a Beijing strain differs from that of another clinical isolate and Mycobacteriu tuberculosis H37Rv 显示文摘 | Carmen P Joanna CB Helen RF | 2005 | Microbiology2005，151，： | 1 |
| 8 | Prognosis value of radionu clide angiography in patients with right ventricular arrhythmias 显示文摘 | Dominique LG Helene G Raphael P | 2001 | Circ2001，103，15： | 1 |
| 9 | Somatostatin - like immunoreactivity in human sympathetic ganglia显示文摘 | Jarvi R Pelto H M Helen P | 1987 | Cell Tissue Res1987，249，1： | 1 |
| 10 | The Geographic Distribution of Production Activity in the UK 显示文摘 | Devereux M P Griffith R Helen Simpson | 1999 | The Institute for Fiscal Studies1999，，26： | 1 |
| 11 | A novel method for producing anti-peptide antibodies显示文摘 | POSNETYZ David N MCGRATH Helen TAM James P | 1988 | The Journal of Biological Chemistry1988，263，4： | 1 |
| 12 | Performance prediction and its use in parallel and distributed computing systems显示文摘 | Stephen A Jarvis Daniel P Spooner Helene N Lim Choi Keung | 2006 | Future Generation Computer Systems2006，22，7： | 1 |
| 13 | Intake, digestibility, and ruminal degradability of shredded hay显示文摘 | Helene Petit V Savoie P et ol | 1994 | J Dairy Sci1994，77，： | 1 |
| 14 | Pilot study assessing functional outcome of tibial pilon fractures using the VSTORM method显示文摘 | Lewis JA Helen V Ian P | 2013 | Injury2013，44，8： | 1 |
| 15 | Increased nuclear factor кB activation critically ill patients who die 显示文摘 | Ross L P Helen F G Jatinde K D | 2000 | Crit Care Med2000，28，： | 1 |
| 16 | High-performance liquid chromatographic resolution of NADP - after induction of fluorescence and its application to assay for an NADPH-dependent enzyme: application to the determination of glutathione reductase activity in p/ant leaf extracts显示文摘 | Helen A N Pillai P | 1996 | Anal Biochem1996，237，1： | 1 |
| 17 | The geographic distribution of production activity in the UK显示文摘 | Michael P Devereux Rachel Griffith Helen Simpson | 2003 | Regional Science and Urban Economics2003，，5： | 1 |
| 18 | Biomass and renewable fuels显示文摘 | Helenal Chum Ralph P Overend | 2001 | Fuel Processing Technology2001，71，3： | 1 |
| 19 | Surface treatment of reinforced concrete in marine environment:influence on chloride diffusion coefficient and capillary water absorption显示文摘 | MEDEIROS M H F HELENE P | 2009 | Construction and Building Materials2009，，23： | 1 |
| 20 | Surface treatment of reinforced concrete in marine environment:influence on chloride diffusion coefficient and capillary water absorption显示文摘 | MEDEIROS M H F HELENE P | 2009 | Construction and Building Materials2009，23，3： | 1 |
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