|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Gastric cancer: Epidemiology, risk factors and prevention strategies显示文摘Gastric cancer(GC) is a global health problem, with more than 1 million people newly diagnosed with GC worldwide each year. GC is more prevalent in less developed countries than in more developed countries. About half of all GC cases worldwide occur in East Asia, notably China. Globally, overall incidence rates of GC are declining, which is potentially attributed to a decrease in Helicobacter pylori(H. pylori) infection and the use of refrigeration to preserve foods rather than salt. GC is a multifactorial disease, and its occurrence and development were impacted by environmental and genetic factors. H. pylori infection is the primary risk factor for GC, especially for non-cardia. The prognosis of GC is poor due to stages at the first diagnosis. The 5-year survival rate is less than 10% when patients are diagnosed at an advanced stage, but the rate is as high as 85% if patients are detected at an earlier stage. Endoscopic screening can potentially prevent GC by early diagnosis and early treatment and has been widely adopted in screening programs in East Asian countries, such as Japan and Korea. This review summarizes updated epidemiological aspects, risk factors, and prevention strategies of GC in recent years to help researchers determine the most effective intervention strategies for reducing risk of GC. | Lei Yang Xiangji Ying Shuo Liu Guoqing Lyu Zekuan Xu Xi Zhang Huichao Li Qingyu Li Ning Wang Jiafu Ji | 2020 | Chinese Journal of Cancer Research2020，32，6： | 52 |
| 3 | Eleven COVID-19 Outbreaks with Local Transmissions Caused by the Imported SARS-CoV-2 Delta VOC-China,July-August,2021显示文摘Starting from December 2019,Wuhan,China,encountered the first outbreak of coronavirus disease 2019(COVID-19)(1-2).The epidemic was successfully suppressed by strict containment so that the number of infected people was reduced to 0 on April 8,2020(3–4).After that,China experienced roughly 3 dozen outbreaks with local transmission caused by imported severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). | Lei Zhou Kai Nie Hongting Zhao Xiang Zhao Bixiong Ye Ji Wang Cao Chen Hong Wang Jiangli Di Jinsong Li Chao Li Zhixiao Chen Ruiqi Ren Peihua Niu Hui Chen Tao Chen Yali Wang Lili Li Bingxue Han Ruhan A Si Chen Dan Li Dan Li Chunxia Jin Xin Zhang Jiangmei Liu Chunyu Xu Yecheng Yao Yenan Feng Zhili Li Bike Zhang Yang Song Peter Hao Yanping Zhang Hao Li Qun Li George F.Gao Guoqing Shi Wenbo Xu | 2021 | China CDC weekly2021，3，41： | 13 |
| 4 | Combined treatment with erythropoietin and granulocyte colony-stimulating factor enhances neovascularization and improves cardiac function after myocardial infarction显示文摘 | Xue Jingyi Du Guoqing Shi Jing Li Yue Masahiro Yasutake Liu Lei Li Jianqiang Kong Yihui Wang Shuxian Yun Fengxiang Li Weimin | 2014 | Chinese Medical Journal2014，，9： | 12 |
| 5 | Leakage performance of floating ring seal in cold/hot state for aero-engine显示文摘Rotating experimental investigations were carried out to study the oil sealing capability of two different floating ring seals in cold/hot state for aero-engine. High-speed Floating Ring Seal(HFRS) is a seal with the inner diameter of 83.72 mm and maximum speed of 38000 r/min, and Low-speed Floating Ring Seal(LFRS) is another seal with the inner diameter of 40.01 mm and maximum speed of 18000 r/min. In hot state, sealing air with the temperature of 371 K and oil with the temperature of 343 K was employed to model the working conditions of an aero-engine. Comparisons between floating ring seal and labyrinth seal were done to inspect the leakage performance.More attention was paid to the critical pressure ratio where the oil leakage began. Results show that the critical pressure ratio in cold state is obviously larger than that in hot state for both seals. An underlying sealing mechanism for floating ring seal is clarified by the fluid film, which closely associates with the dimensionless parameter of clearance over rotating diameter(2 c/Dr). Another fantastic phenomenon is that the leakage coefficient in hot state, not the leakage magnitude, is unexpectedly larger than that in cold state. Overall, the leakage performance of the floating ring seal is better than the labyrinth seal. | Guoqing LI Qian ZHANG Enliang HUANG Zhijun LEI Hongwei WU Gang XU | 2019 | Chinese Journal of Aeronautics2019，32，9： | 9 |
| 6 | An analytical study of unsteady heat transfer in the rock surrounding a deep airway显示文摘The heat transfer between an airway and the air flowing though it is an unsteady problem. The governing equation of unsteady heat transfer was solved using the method of separation of variables. The solution is an infinite series including Bessel functions. The theoretical solution was analyzed by solving for the positive roots of the transcendental equation by iteration. The dimensionless surface temperature of the sur- rounding rock is only affected by the Bi number but not by the thermo-physical coefficients of the rock. The dimensionless coefficient of heat transfer, k, decreases with the Fo number similarly to the influence of the Bi number on k. A formula for determining the fully developed stage (FDS) suitable for unsteady heat transfer in the airway is proposed. The FDS from theoretical analysis occurs with Fo from 1.6 to 2. The ratio of excess temperature in the surrounding rock is independent of the initial conditions and only dependent on the Bi number and the relative position in the airway, at the FDS. The calculation error is large when using just the first term from the complete series when Fo is from 2 to 12. Five terms give a solution approximately equal to that found using the complete series. The first term could replace the complete series only when Fo is greater than 12. The FDS plays an important role in predication of the temperature field of the surrounding rock and in simplified calculations. | Wang Yijiang | 2012 | International Journal of Mining Science and Technology2012，22，3： | 4 |
| 7 | Elastic Scattering of 7Be on Pb Target显示文摘 | Yang Yanyun Wang Jiansong Wang Qi Huang Meirong Ma Junbing Ma Peng Cao Xiguang Han Jianlong Bai Zhen Jin Shilong Hu Qiang Jin Lei Chen Jiangbo Zhang Xueying Chen Ruofu Hu Zhengguo Xu Shiwei Zhen Chuan Xu Zhiguo Xu Hushan Duan Liming Sun Zhiyu Chen Zhiqiang He Jianjun Li Songlin Lei Xiangguo Fu Fen Zhang Xueheng Yuan Xiaohua Du Chengming Yan Xinshuang Hu Jun Tang Shuwen Ye Ruiping Yang Kun Shi Fudong Zhang Wei Chen Ze Li Long Xu Xing Liu Longxiang Zhang Liyong Lin Qingyun Gou Boxing Xu Ge Zhou Xionghong Zhang Yuhu Xiao Guoqing | 2011 | IMP & HIRFL Annual Report2011，，1： | 4 |
| 8 | Alterations in cecal microbiota of Jinhua piglets fostered by a Yorkshire sow显示文摘Gut microbiota play important roles in host health and diseases.Several factors,in particular antibiotics,affect the gut microbiota of pigs.Cross-fostering has been applied as a regular practice to equalize litter size,reduce pre-weaning mortality and increase body weight.However,the effect of cross-fostering on cecal microbiota is unclear.In this study,we fostered three Jinhua pigs to a Yorkshire sow.The fostered Jinhua piglets grew significantly faster than their biological siblings.To explore whether the cecal microbiota of piglets will alter during fostering,we characterized the cecal microbiota of allpiglets by examining the V3 hypervariable region of 16S rDNA.We observed altered cecal microbiota in these piglets using the Illumina HiSeq 2000 platform,and this was accompanied with an increase in growth rate after fostering.The relative abundance of Bacteroidetes in the fostered Jinhua piglets was decreased compared with their biological siblings,although still higher in comparison with their new littermates.Beta-diversity analysis also showed that the cecal microbiota of the adopted Jinhua piglets differed from their biological siblings with a shift toward their step-siblings.Our data show that cecal microbiota of piglets were altered after cross-fostering while the growth rate increased. | Lingjin Xian Ying Li Zhi Jiang Jideng Ma Long Jin Lei Chen Chaowei Zhou Jie Zhang Yingkai Liu Li Zhu An-an Jiang Guoqing Tang Jian Xiao Jinyong Wang Bo Zeng Mingzhou Li Xuewei Li | 2014 | Chinese Science Bulletin2014，59，32： | 4 |
| 9 | Sensitivity analysis of the SWAP(Soil-Water-Atmosphere-Plant)model under different nitrogen applications and root distributions in saline soils显示文摘Sensitivity analysis is important for determining the parameters in the model calibration process.In our study,a variance-based global sensitivity analysis(extended Fourier amplitude sensitivity test,EFAST)was applied to an agro-hydrological model(the SWAP(Soil-Water-Atmosphere-Plant model)model).The sensitivities of 20 parameters belonging to 4 categories(soil hydraulics,solute transport,root water uptake,and environmental stresses)for the simulated accumulated transpiration,dry matter(DM),and yield of sunflowers were analyzed under three nitrogen application rates(N1,N2,and N3),four salinity levels(S1,S2,S3,and S4),and three root distributions(R1,R2,and R3).The results indicated that for predominantly loamy soils,the high-impact parameters for accumulated transpiration,DM,and yield were the soil hydraulic parameters(αand n),critical stress index for compensatory root water uptake(ωc),the salt level at which salt stress starts(Pi),the decline of root water uptake above Pi(SSF),residual water content(θr),saturated water content(θs),and relative uptake of solutes by roots(TSCF).We also found that nitrogen application did not change the order of the parameter impacts on accumulated transpiration,DM,and yield.However,TSCF replacedαas the highest-impact parameter for the accumulated transpiration,DM,and yield at high salinity levels(S2 and S3).Furthermore,αwas also the highest-impact parameter for DM and yield under different root distributions,but the highest-impact parameters for transpiration wereωc,α,andθs under R1,R2,and R3,respectively.Nitrogen application could be neglected when considering the interactive effects of nitrogen application,salinity level,and root distribution on the transpiration,DM,and yield.Additionally,the mean values and uncertainties of the transpiration,DM,and yield were similar in all scenarios,except S3,which showed a sharp decrease in the mean values.We suggest determining the above eight parameters(α,n,ωc,Pi,SSF,θr,θs,and TSCF)and the saturated vertical hydraulic conductivity(Ks)based on rigorous calibrations with direct or indirect local measurements using economical methods(e.g.,a literature review),with limited observations for other parameters when using the SWAP model and other similar agro-hydrological models. | Guoqing LEI Wenzhi ZENG Yonghua JIANG Chang AO Jingwei WU Jiesheng HUANG | 2021 | Pedosphere2021，31，5： | 4 |
| 10 | A wireless, implantable optoelectrochemical probe for optogenetic stimulation and dopamine detection显示文摘Physical and chemical technologies have been continuously progressing advances in neuroscience research.The development of research tools for closed-loop control and monitoring neural activities in behaving animals is highly desirable.In this paper,we introduce a wirelessly operated,miniaturized microprobe system for optical interrogation and neurochemical sensing in the deep brain.Via epitaxial liftoff and transfer printing,microscale light-emitting diodes(micro-LEDs)as light sources and poly(3,4-ethylenedioxythiophene)polystyrene sulfonate(PEDOT:PSS)-coated diamond films as electrochemical sensors are vertically assembled to form implantable optoelectrochemical probes for real-time optogenetic stimulation and dopamine detection capabilities.A customized,lightweight circuit module is employed for untethered,remote signal control,and data acquisition.After the probe is injected into the ventral tegmental area(VTA)of freely behaving mice,in vivo experiments clearly demonstrate the utilities of the multifunctional optoelectrochemical microprobe system for optogenetic interference of place preferences and detection of dopamine release.The presented options for material and device integrations provide a practical route to simultaneous optical control and electrochemical sensing of complex nervous systems. | Changbo Liu Yu Zhao Xue Cai Yang Xie Taoyi Wang Dali Cheng Lizhu Li Rongfeng Li Yuping Deng He Ding Guoqing Lv Guanlei Zhao Lei Liu Guisheng Zou Meixin Feng Qian Sun Lan Yin Xing Sheng | 2020 | Microsystems & Nanoengineering2020，6，1： | 3 |
| 11 | Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR显示文摘The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1,but also provide the strategies to monitor other new types ofinfluenza virus with explosive potential. | YAN An DING GuoHui ZHOU ZhenFeng DONG Hui ZHANG YaKun ZHU Lei HE YunGang ZHANG GuoQing LI YiXue SUN Bing HUANG Zhong LAN Ke JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenGan ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN WeiRong XIONG Hui | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 12 | Effects of swirler position on flame response and combustion instabilities显示文摘This study is concerned with the experimental and theoretical investigation of the combustion instabilities in a premixed swirl combustor.It is focused on the effects of the swirl mixing distance on the intrinsic thermoacoustic mode.The swirler as an origin of the swirling flow is also the source of the flow disturbance,which has effects on the flame response.The location of the swirler is varied in the experiment to study the effect on combustion instabilities and flame transfer functions.A low order model is built to analyze the thermoacoustic instabilities of the combustion system.The experimental results show that the ITA switches from an unstable state to a stable state as the swirl mixing distance changes with an increment of 15 mm;while the instability of the quarter-wave mode is not varied.The measured Flame Transfer Functions(FTFs)show that the gain curves of the frequency-dependent FTFs seem to be stretched or compressed with the modulation of the swirler position,which has effects on frequencies and instabilities of thermoacoustic modes.With the low order model,the effects of flame response on combustion instabilities are analyzed and the flame dominant nature of the ITA mode is confirmed. | Liangliang XU Jianyi ZHENG Guoqing WANG Lei LI Fei QI | 2022 | Chinese Journal of Aeronautics2022，35，3： | 2 |
| 13 | Consideration of Monkeypox Surveillance in China,2022显示文摘Since the first monkeypox cases were confirmed in England in May 2022(1),100 countries worldwide have reported 53,027 confirmed cases and 15 deaths as of September 2(2).This is the largest and most widespread monkeypox epidemic in the world since monkeypox virus was discovered and identified in 1958,involving the largest number of countries and transmitting primarily in non-endemic countries(3).Moreover,the currently ongoing monkeypox epidemic also has some characteristics that are different from previous outbreaks(4).For example,most cases have a history of sexual contact,with an overwhelming majority of men who have sex with men(MSM)(5).On July 23,the WHO declared the global monkeypox outbreak a Public Health Emergency of International Concern(PHEIC)in order to address the ongoing outbreak with the required urgency(6). | Lei Zhou Yali Wang Dan Li Mengjie Han Guoqing Shi Qun Li | 2022 | China CDC weekly2022，4，41： | 2 |
| 14 | Leakage performance of labyrinth seal for oil sealing of aero-engine显示文摘Experimental investigation has been done to evaluate the leakage performance of labyrinth seal for oil sealing on high-speed sealing test rig at different working and geometric parameters.Typical values of pressure ratio ranging from 1.0 to 2.0 were used and the rotating speed varied from 0 to 30,000 tpm.Dimensionless Taylor number was invited to response the effect of rotation.Oil was injected at the rate from 1.2 L/min to 2.8 L/min to check the sealing capacity.Leakage was measured at different seal configurations including sealing clearance,tooth tip thickness,pitch,teeth number,front inclined angle and oil-throwing angle.Different from gas sealing,the application of oil-throwing tooth in oil sealing attracted much interest as an obvious alternative to the conventional labyrinth seal.A blocking ring was captured during testing,which establishes understanding of underlying flow mechanisms in the clearance and plays an important role in oil sealing.There is a critical Taylor number at which the leakage coefficient drops drastically.After the critical Taylor number,a parabola rule appears.An optimal composition of tooth tip thickness,teeth number,oil-throwing angle and front inclined angle exists where the leakage performance behaves better. | Guoqing Li Qian Zhang Zhijun Lei Enliang Huang Hongwei Wu Gang Xu | 2019 | Propulsion and Power Research2019，8，1： | 2 |
| 15 | Prognostic significance of periostin in colorectal cancer显示文摘Accumulating evidence suggests that periostin is frequently upregulated in tissue injury, inflammation,fibrosis and tumor progression. Periostin expression in cancer cells can promote metastatic potential of colorectal cancer(CRC)via activating PI3 K/Akt signaling pathway. Moreover, periostin is observed mainly in tumor stroma and cytoplasm of cancer cells, which may facilitate aggressiveness of CRC. In this review, we summarize information regarding periostin to emphasize its role as a prognostic marker of CRC. | Xingming Deng Sheng Ao Jianing Hou Zhuofei Li Yunpeng Lei Guoqing Lyu | 2019 | Chinese Journal of Cancer Research2019，31，3： | 2 |
| 16 | Warm dense matter research at HIAF显示文摘The research activities on warm dense matter driven by intense heavy ion beams at the new project High Intensity heavy-ion AcceleratorFacility (HIAF) are presented. The ion beam parameters and the simulated accessible state of matter at HIAF are introduced, respectively. Theprogresses of the developed diagnostics for warm dense matter research including high energy electron radiography, multiple-channel pyrometer,in-situ energy loss and charge state of ion detector are briefly introduced. | Rui Cheng Yu Lei Xianming Zhou Yuyu Wang Yanhong Chen Yongtao Zhao Jieru Ren Lina Sheng Jiancheng Yang Zimin Zhang Yingchao Du Wei Gai Xinwen Ma Guoqing Xiao | 2018 | Matter and Radiation at Extremes2018，3，2： | 2 |
| 17 | Validation of the simplified criteria for diagnosis of autoimmune hepatitis in Chinese patients显示文摘 | Dekai Qiu Qixia Wang Hui Wang Qing Xie Guoqing Zang Hong Jiang Chuantao Tu Jinsheng Guo Shuncai Zhang Jianshe Wang Yi Lu Ying Han Lei Shen Xiaoyu Chen Xiqi Hu Xiaojin Wang Chengwei Chen Qingchun Fu Xiong Ma | 2010 | Journal of Hepatology2010，，： | 2 |
| 18 | Evolutionary relationship of three mitten crabs(Eriocheir sp)revealed by mitogenome and 5S ribosomal DNA analysis显示文摘To reveal the evolutionary relationship of three mitten crabs(Eriocheir sinensis,E.hepuensis,and E.japonica),complete mitogenomes and nuclear 5S rDNA sequences were analyzed.Sequencing revealed that the mitogenomes analyzed shared conserved organization of the coding and non-coding regions but genetic variation was identified.Among the three mitten crabs distinct tandem repeats were identified in the mitochondrial D-loop region.The 5S gene(5S rDNA)sequence was highly conserved across the three species,whereas non-transcribed spacer(NTS)region exhibit high levels of variation including insertions,deletions and point mutations.Cluster analysis suggested that the three mitten crabs had their own independent 5S rDNA sequence variation and evolutionary pattern.Both mitogenome and 5S rDNA sequence analysis revealed significant genetic variation across the mitten crabs species.Phylogenetic analysis using mitogenome and 5S rDNA sequences demonstrated that E.japonica was relatively more distant from E.sinensis and E.hepuensis.This study extended our previous knowledge and confirmed that the three mitten crabs are likely to be genetically differentiated species.In addition,our study also provided insights into the conservation of pure natural resources of E.sinensis,an important aquaculture species. | Xiaowen Chen Jun Wang Lei Huang Wucheng Yue Jixing Zou Chunhong Yuan Guoqing Lu Chenghui Wang | 2017 | Aquaculture and Fisheries2017，2，6： | 1 |
| 19 | Relationship between Ti/Al ratio and stress-rupture properties in nickel-based superalloy显示文摘 | Yulai Xu Lei Zhang Jun Li Xueshan Xiao Xiuli Cao Guoqing Jia Zhi Shen | 2012 | Materials Science & Engineering A2012，，： | 1 |
| 20 | Spectroscopic studies on the excited state properties of the light-induced antiviral drug hypocrellin A loaded in the mesoporous solid显示文摘 | Lei Z Zhang Guoqing Tang Bowen Gao | 2004 | Chem Phys Lett2004，396，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员