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| 1 | Stable classi?cation with limited sample: transferring a 30-m resolution sample set collected in 2015 to mapping 10-m resolution global land cover in 2017显示文摘As the world strives to reduce the impact of population growth, urbanization, agricultural expansion, and climate change on food security, energy and water shortage, resource over-exploration, biodiversity loss, environmental pollution, and ultimately human health, timely and higher resolution land cover information is urgently needed to achieve the sustainable development goals of the United Nations. | Peng Gong Han Liu Meinan Zhang Congcong Li Jie Wang Huabing Huang Nicholas Clinton Luyan Ji Wenyu Li Yuqi Bai Bin Chen Bing Xu Zhiliang Zhu Cui Yuan Hoi Ping Suen Jing Guo Nan Xu Weijia Li Yuanyuan Zhao Jun Yang Chaoqing Yu Xi Wang Haohuan Fu Le Yu Iryna Dronova Fengming Hui Xiao Cheng Xueli Shi Fengjin Xiao Qiufeng Liu Lianchun Song | 2019 | Science Bulletin2019，64，6： | 166 |
| 2 | OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1显示文摘WRKY family proteins are a class of plant specific transcription factors that involve in many stress response pathways. It has been shown that one Arabidopsis WRKY protein, AtWRKY29/22, is activated by MAP kinase signaling cascade and confers resistance to both bacterial and fungal pathogens. However, little is known about the biological roles of WRKY proteins in rice. In this study, we investigated the expression patterns of rice AtWRKY29/22 homolog, OsWRKY03, under different conditions, and also its possible role involved in plant defense. Our results showed that OsWRKY03 was up-regulated by several defense signaling molecules or different treatments. Further analysis revealed that the expres- sion of OsWRKY03 was light dependent. Transcriptional activation activity of OsWRKY03 was also demonstrated by yeast functional assay. Transient expression of OsWRKY03-GFP fusion protein in onion epidermis cells showed that OsWRKY03 was a nuclear localized protein. OsNPR1 as well as several other pathogenesis-related genes, such as OsPR1b, phenylalanine ammonia-lyase (ZB8) and peroxidase (POX22.3), were induced in OsWRKY03-overexpressing transgenic plants. These results indicated that OsWRKY03 is located upstream of OsNPR1 as a transcriptional activator in salicylic acid (SA)-dependent or jasmonic acid (JA)-dependent defense signaling cascades. | Xiao Qiang LIU Xian Quan BAI Qian QIAN Xiu Jie WANG Ming Sheng CHEN Cheng Cai CHU | 2005 | Cell Research2005，15，8： | 55 |
| 3 | Programmable time-domain digital-coding metasurface for non-linear harmonic manipulation and new wireless communication systems显示文摘Optical non-linear phenomena are typically observed in natural materials interacting with light at high intensities, and they benefit a diverse range of applications from communication to sensing. However,controlling harmonic conversion with high efficiency and flexibility remains a major issue in modern optical and radio-frequency systems. Here, we introduce a dynamic time-domain digital-coding metasurface that enables efficient manipulation of spectral harmonic distribution. By dynamically modulating the local phase of the surface reflectivity, we achieve accurate control of different harmonics in a highly programmable and dynamic fashion, enabling unusual responses, such as velocity illusion. As a relevant application, we propose and realize a novel architecture for wireless communication systems based on the time-domain digital-coding metasurface, which largely simplifies the architecture of modern communication systems, at the same time yielding excellent performance for real-time signal transmission. The presented work, from new concept to new system, opens new pathways in the application of metamaterials to practical technology. | Jie Zhao Xi Yang Jun Yan Dai Qiang Cheng Xiang Li Ning Hua Qi Jun Chen Ke Guo Dong Bai Shuo Liu Shi Jin Andrea Alù Tie Jun Cui | 2019 | National Science Review2019，6，2： | 46 |
| 4 | Effects of long-term full straw return on yield and potassium response in wheat-maize rotation显示文摘The effect of long-term straw return on crop yield, soil potassium(K) content, soil organic matter, and crop response to K from both straw and chemical K fertilizer(K_2SO_4) were investigated in a fixed site field experiment for winter wheat-summer maize rotation in 6 years for 12 seasons. The field experiment was located in northern part of North China Plain with a sandy soil in relatively low yield potential. Two factors, straw return and chemical K fertilizer, were studied with two levels in each factor. Field split design was employed, with two straw treatments, full straw return of previous crop(St) and no straw return, in main plots, and two chemical K fertilizer treatments, 0 and 60 kg K2 O ha^(–1), as sub-plots. The results showed that straw return significantly increased yields of winter wheat and summer maize by 16.5 and 13.2% in average, respectively, and the positive effect of straw return to crop yield showed more effective in lower yield season. Straw return significantly increased K absorption by the crops, with significant increase in straw part. In treatment with straw return, the K content in crop straw increased by 15.9 and 21.8% in wheat and maize, respectively, compared with no straw return treatment. But, straw return had little effect on K content in grain of the crops. Straw return had significant influences on total K uptake by wheat and maize plants, with an increase of 32.7 and 30.9%, respectively. There was a significant correlation between crop yield and K uptake by the plant. To produce 100 kg grain, the wheat and maize plants absorbed 3.26 and 2.24 kg K2 O, respectively. The contents of soil available K and soil organic matter were significantly affected by the straw return with an increase of 6.07 and 23.0%, respectively, compared to no straw return treatment. K_2SO_4 application in rate of 60 kg K2 O ha^(–1) showed no significant effect on wheat and maize yield, K content in crop straw, total K uptake by the crops, soil available K content, and soil organic matter. The apparent K utilization rate(percentage of applied K absorbed by the crop in the season) showed difference for wheat and maize with different K sources. In wheat season, the K utilization rate from K_2SO_4 was higher than that from straw, while in maize season, the K utilization rate from straw was higher than that from chemical fertilizer. In the whole wheat-maize rotation system, the K absorption efficiency by the two crops from straw was higher than that from K_2SO_4. | BAI You-lu WANG Lei LU Yan-li YANG Li-ping ZHOU Li-ping NI Lu CHENG Ming-fang | 2015 | Journal of Integrative Agriculture2015，14，12： | 35 |
| 5 | Apelin-13 inhibits apoptosis and excessive autophagy in cerebral ischemia/reperfusion injury显示文摘Apelin-13 is a novel endogenous ligand for an angiotensin-like orphan G-protein coupled receptor,and it may be neuroprotective against cerebral ischemia injury.However,the precise mechanisms of the effects of apelin-13 remain to be elucidated.To investigate the effects of apelin-13 on apoptosis and autophagy in models of cerebral ischemia/reperfusion injury,a rat model was established by middle cerebral artery occlusion.Apelin-13(50μg/kg)was injected into the right ventricle as a treatment.In addition,an SH-SY5Y cell model was established by oxygen-glucose deprivation/reperfusion,with cells first cultured in sugar-free medium with 95%N2 and 5%CO2 for 4 hours and then cultured in a normal environment with sugar-containing medium for 5 hours.This SH-SY5Y cell model was treated with 10-7 M apelin-13 for 5 hours.Results showed that apelin-13 protected against cerebral ischemia/reperfusion injury.Apelin-13 treatment alleviated neuronal apoptosis by increasing the ratio of Bcl-2/Bax and significantly decreasing cleaved caspase-3 expression.In addition,apelin-13 significantly inhibited excessive autophagy by regulating the expression of LC3B,p62,and Beclin1.Furthermore,the expression of Bcl-2 and the phosphatidylinositol-3-kinase(PI3K)/Akt/mammalian target of rapamycin(mTOR)pathway was markedly increased.Both LY294002(20μM)and rapamycin(500 nM),which are inhibitors of the PI3K/Akt/mTOR pathway,significantly attenuated the inhibition of autophagy and apoptosis caused by apelin-13.In conclusion,the findings of the present study suggest that Bcl-2 upregulation and mTOR signaling pathway activation lead to the inhibition of apoptosis and excessive autophagy.These effects are involved in apelin-13-induced neuroprotection against cerebral ischemia/reperfusion injury,both in vivo and in vitro.The study was approved by the Animal Ethical and Welfare Committee of Jining Medical University,China(approval No.2018-JS-001)in February 2018. | Zi-Qi Shao Shan-Shan Dou Jun-Ge Zhu Hui-Qing Wang Chun-Mei Wang Bao-Hua Cheng Bo Bai | 2021 | Neural Regeneration Research2021，16，6： | 35 |
| 6 | Guidance for the clinical evaluation of traditional Chinese medicine-induced liver injury Issued by China Food and Drug Administration显示文摘1. Introduction Drug-induced liver injury (DILI), defined as liver injury caused by a drug and/or its metabolites, is a common clinical adverse drug reaction1–4. This type of injury can cause acute liver failure and even death in severe cases5. | Xiaohe Xiao Jianyuan Tang Yimin Mao Xiuhui Li Jiabo Wang Chenghai Liu Kewei Sun Yong’an Ye Zhengsheng Zou Cheng Peng Ling Yang Yuming Guo Zhaofang Bai Tingting He Jing Jing Fengyi Li Na An | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 22 |
| 7 | Nickel-exposed Workers in China: A Cohort Study显示文摘There are more than 50 000 workers in Jinchuan Group Co,Ltd(JNMC).Since all staff in JNMC are eligible for a medical examination every two years,only 23 484 nickel-exposed subjects who participated in medical examination were included in this study.Their data,collected from June 22,2011 to September 28,2012,in a | BAI Ya Na YAGN Ai Min PU Hong Quan HE Jie CHENG Ning ZHENG Tong Zhang DAI Min ZHANG Ya Wei BASSING Bryan WANG Qiu Ying | 2014 | Biomedical and Environmental Sciences2014，27，3： | 20 |
| 8 | Solitary pulmonary nodules: comparison of multi-slice computed tomography perfusion study with vascular endothelial growth factor and microvessel density显示文摘背景独居的肺的小瘤(SPN ) 是胸拍上的最普通的调查结果之一。临床的实践的目的是以最少的侵略方法把恶意的小瘤区分开来与良性的小瘤并且做特定的诊断。这研究被瞄准用多片在 SPN 评估在灌注成像特征和 microvessel 密度和脉管的 endothelial 生长因素(VEGF )(MVD ) 之间的关联计算断层摄影术(MSCT ) ;并且流动模式和血流动为 SPN 血提供理论基础量的 features.Also ,在良性、恶意的 SPNs.Methods 的微分诊断为方法的临床的申请价值的讨论叫的学习有 SPN 的 68 个病人经历了动态对比提高了的多地点( nonionic 对比材料以 4 ml/s 的率经由 antecubital 静脉被管理) MSCT 。每扫描上的 Precontrast 和 postcontrast 变细被学习。到主动脉的 SPN 的山峰高度的灌注,山峰高度,和比率被分析。灌注用时间密度曲线(TDC ) 和主动脉的山峰高度的最大的坡度被计算。量的参数(灌注,山峰高度,到主动脉的 SPN 的山峰高度的比率) 血流动,模式与 MVD 和 immunohistochemistry.Results 的 VEGF 表达式相比灌注山峰高度恶意((96. | BAI Rong-jie CHENG Xiao-guang QU Hui SHEN Bao-zhong HAN Ming-jun WU Zhen-hua | 2009 | Chinese Medical Journal2009，，5： | 19 |
| 9 | Advanced Space-based Solar Observatory(ASO-S):an overview显示文摘The Advanced Space-based Solar Observatory(ASO-S)is a mission proposed for the 25 th solar maximum by the Chinese solar community.The scientific objectives are to study the relationships between the solar magnetic field,solar flares and coronal mass ejections(CMEs).Three payloads are deployed:the Full-disk vector Magneto Graph(FMG),the Lyman-αSolar Telescope(LST)and the Hard X-ray Imager(HXI).ASO-S will perform the first simultaneous observations of the photospheric vector magnetic field,non-thermal imaging of solar flares,and the initiation and early propagation of CMEs on a single platform.ASO-S is scheduled to be launched into a 720 km Sun-synchronous orbit in 2022.This paper presents an overview of the mission till the end of Phase-B and the beginning of Phase-C. | Wei-Qun Gan Cheng Zhu Yuan-Yong Deng Hui Li Yang Su Hai-Ying Zhang Bo Chen Zhe Zhang Jian Wu Lei Deng Yu Huang Jian-Feng Yang Ji-Jun Cui Jin Chang Chi Wang Ji Wu Zeng-Shan Yin Wen Chen Cheng Fang Yi-Hua Yan Jun Lin Wei-Ming Xiong Bin Chen Hai-Chao Bao Cai-Xia Cao Yan-Ping Bai Tao Wang Bing-Long Chen Xin-Yu Li Ye Zhang Li Feng Jiang-Tao Su Ying Li Wei Chen You-Ping Li Ying-Na Su Hai-Yan Wu Mei Gu Lei Huang Xue-Jun Tang | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 17 |
| 10 | Qingfei Xiaoyan Wan,a traditional Chinese medicine formula, ameliorates Pseudomonas aeruginosa–induced acute lung inflammation by regulation of PI3K/AKT and Ras/MAPK pathways显示文摘Gram-negative pathogen–induced nosocomial infections and resistance are a most serious menace to global public health. Qingfei Xiaoyan Wan(QF), a traditional Chinese medicine(TCM)formula, has been used clinically in China for the treatment of upper respiratory tract infections, acute or chronic bronchitis and pulmonary infection. In this study, the effects of QF on Pseudomonas aeruginosa–induced acute pneumonia in mice were evaluated. The mechanisms by which four typical antiinflammatory ingredients from QF, arctigenin(ATG), cholic acid(CLA), chlorogenic acid(CGA) and sinapic acid(SPA), regulate anti-inflammatory signaling pathways and related targets were investigated using molecular biology and molecular docking techniques. The results showed that pretreatment with QF significantly inhibits the release of cytokines(TNF-α and IL-6) and chemokines(IL-8 and RANTES),reduces leukocytes recruitment into inflamed tissues and ameliorates pulmonary edema and necrosis. In addition, ATG was identified as the primary anti-inflammatory agent with action on the PI3K/AKT and Ras/MAPK pathways. CLA and CGA enhanced the actions of ATG and exhibited synergistic NF-κB inactivation effects possibly via the Ras/MAPK signaling pathway. Moreover, CLA is speculated to target FGFR and MEK firstly. Overall, QF regulated the PI3K/AKT and Ras/MAPK pathways to inhibit pathogenic bacterial infections effectively. | Yuanyuan Hou Yan Nie Binfeng Cheng Jin Tao Xiaoyao Ma Min Jiang Jie Gao Gang Bai | 2016 | Acta Pharmaceutica Sinica B2016，6，3： | 17 |
| 11 | Methylation of Dickkopf-3 as a prognostic factor in cirrhosis-related hepatocellular carcinoma显示文摘AIM:To investigate the prevalence and time of Dickkopf(DKK) family methylation and its clinical significance in hepatocarcinogenesis. METHODS:Methylation of DKK family genes was quantitatively analyzed in 115 liver tissue samples,including 50 pairs of primary hepatocellular carcinoma(HCC) and matched noncancerous cirrhotic tissue samples,as well as 15 liver cirrhosis biopsy samples. RESULTS:The methylation level of DKK3 was significantly higher in HCC tissue samples than in matched noncancerous cirrhotic tissue samples(P<0.0001) orin liver cirrhosis biopsy samples(P=0.0139) .Receiver operator characteristic curve analysis confirmed that the percent of methylated reference(PMR) values of DKK3 could effectively discriminate HCC tissue samples from noncancerous tissue samples(AUC=0.8146) or liver cirrhosis biopsy samples(AUC=0.7093) .Kaplan-Meier survival curves revealed that the progression-free survival time of patients with a higher DKK3 methylation level(PMR >1%) was significantly shorter than that of those with a lower DKK3 methylation level(PMR≤1%)(P=0.0255) . Multivariate Cox analysis indicated that methylated DKK3 was significantly and independently related with a shorter survival time(relative risk=2.527,95%CI:1.063-6.008,P=0.036) of HCC patients. CONCLUSION:Methylation of DKK3 is an important event in early malignant transformation and HCC progression,and therefore might be a prognostic indicator for risk assessment of HCC. | Yang, Bin Du, Zhi Gao, Ying-Tang Lou, Cheng Zhang, Shi-Guang Bai, Tong Wang, Yi-Jun Song, Wen-Qin | 2010 | World Journal of Gastroenterology2010，16，6： | 16 |
| 12 | Characteristics of paleoflood deposits archived in unit T0403 of Yuxi Site in the Three Gorges reservoir areas,China显示文摘Based on the detailed analyses,multi-proxies such as AMS 14 C dating,grain size,component and morphology of heavy minerals,micromorphology of zircon,magnetic susceptibility,Rb/Sr and Hg content are used to research the paleoflood sediments archived in the Yuxi Site.The research indicates that, since 7.6 kaBP,at least 16 times of paleoflood with water level above Wusong Elevation 147.024 m(a.s.l.) left deposits in the Neolithic layers of unit T0403 from the Yuxi Site.The results are induced from the following aspects:(1)The plaeoflood sediments take on great similarities with modern flood sediments in the Yuxi Site and Zhongba Site in probability cumulative curves which mainly show a pattern of 3 segments in fluvial pattern.(2)There is some resemblance between the heavy mineral components and the zircon shape characteristics of paleoflood deposits and those of modern flood deposits.(3) Magnetic susceptibility values(40.44―70.10SI)are lower than those of sediments from cultural layers (59.59―188.68SI).(4)Hg values(290.71―742.51 ng/g)are lower than those of sediments from cultural layers(344.16―10518.17 ng/g).(5)Rb/Sr values are higher than those of sediments from cultural layers, while those of the 4th,5th,6th,7th,8th cultural layers are high,which shows that they are inundated by paleoflood.The reason for many flood deposits existing is related to the site situated on the first terrace where the Yuxi River joins with the Yangtze River.As there are some similarities between the plaeoflood sediments and those of the Zhongba Site,it is feasible to confirm the existence of paleoflood sediments based on the above points. | ZHU Cheng MA ChunMei XU WeiFeng BAI JiuJiang ZHENG ChaoGui ZHU GuangYao WANG HuiLin CHEN Ye LU XueFeng | 2008 | Chinese Science Bulletin2008，53，S1： | 16 |
| 13 | Echinacoside Protects Against MPP+-Induced Neuronal Apoptosis via ROS/ATF3/CHOP Pathway Regulation显示文摘Echinacoside(ECH) is protective in a mouse model of Parkinson's disease(PD) induced by 1-methyl-4-phenylpyridinium ion(MPP^+). To investigate the mechanisms involved, SH-SY5Y neuroblastoma cells were treated with MPP^+or a combination of MPP^+and ECH,and the expression of ATF3(activating transcription factor3), CHOP(C/EBP-homologous protein), SCNA(synuclein alpha), and GDNF(glial cell line-derived neurotrophic factor) was assessed. The results showed that ECH significantly improved cell survival by inhibiting the generation of MPP^+-induced reactive oxygen species(ROS). In addition, ECH suppressed the ROS and MPP^+-induced expression of apoptotic genes(ATF3, CHOP, and SCNA). ECH markedly decreased the MPP^+-induced caspase-3 activity in a dose-dependent manner. ATF3-knockdown also decreased the CHOP and cleaved caspase-3 levels and inhibited the apoptosis induced by MPP^+.Interestingly, ECH partially restored the GDNF expression that was down-regulated by MPP^+. ECH also improved dopaminergic neuron survival during MPP^+treatment and protected these neurons against the apoptosis induced by MPTP. Taken together, these data suggest that the ROS/ATF3/CHOP pathway plays a critical role in mechanisms by which ECH protects against MPP^+-induced apoptosis in PD. | Qing Zhao Xiaoyan Yang Dingfang Cai Ling Ye Yuqing Hou Lijun Zhang Jiwei Cheng Yuan Shen Kaizhe Wang Yu Bai | 2016 | Neuroscience Bulletin2016，32，4： | 14 |
| 14 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 15 | Smart metasurface with self-adaptively reprogrammable functions显示文摘Intelligence at either the material or metamaterial level is a goal that researchers have been pursuing.From passive to active,metasurfaces have been developed to be programmable to dynamically and arbitrarily manipulate electromagnetic(EM)wavefields.However,the programmable metasurfaces require manual control to switch among different functionalities.Here,we put forth a smart metasurface that has self-adaptively reprogrammable functionalities without human participation.The smart metasurface is capable of sensing ambient environments by integrating an additional sensor(s)and can adaptively adjust its EM operational functionality through an unmanned sensing feedback system.As an illustrative example,we experimentally develop a motion-sensitive smart metasurface integrated with a three-axis gyroscope,which can adjust self-adaptively the EM radiation beams via different rotations of the metasurface.We develop an online feedback algorithm as the control software to make the smart metasurface achieve single-beam and multibeam steering and other dynamic reactions adaptively.The proposed metasurface is extendable to other physical sensors to detect the humidity,temperature,illuminating light,and so on.Our strategy will open up a new avenue for future unmanned devices that are consistent with the ambient environment. | Qian Ma Guo Dong Bai Hong Bo Jing Cheng Yang Lianlin Li Tie Jun Cui | 2019 | Light(Science & Applications)2019，8，1： | 13 |
| 16 | Role of Fas/FasL Pathway-mediated Alveolar Macrophages Releasing Inflammatory Cytokines in Human Silicosis显示文摘The role of Fas/Fas ligand (FasL) in mediating alveolar macrophage (AM)-derived inflammatory cytokines in 29 human lung silicosis patients and 6 controls was studied.It showed that the serum levels of IL-1β,TNF-α,IL-8,MIP-1α,MCP-1,and TGF-β1were higher in silicosis patients than in controls,and increased with the progression of silicosis except TGF-β1 and IL-8.TGF-β1,IL-8,MCP-1,MIP-1αwere efficiently down-regulated by anti-FasL antibody or caspase-8 inhibitor,indicating that Fas/FasL signaling promotes the | YAO San Qiao HE Qin Cheng YUAN Ju Xiang CHEN Jie CHEN Gang LU Yao BAI Yu Ping ZHANG Chun Min YUAN Yang XU Ying Jun | 2013 | Biomedical and Environmental Sciences2013，26，11： | 13 |
| 17 | Influence of hepatic arterial blockage on blood perfusion and VEGF,MMP-1 expression of implanted lver cancer in rats显示文摘AIM:To investigate the influence of hepatic arterial blodckage on blood perfusion of transplanted cancer in rat liver and the expression of vascular endothelial growth factor (VEGF)and matrix metalloproteinase-1(MMP-1),and to explore the mechanisms involved in transarterial embolization(TAE)-induced metastasis of liver cancer preliminarily.METHODS:Walker 256 carcinosarcoma was transplanted in to rat liver to establish the liver cancer model.Hepatic arterial ligation(HAL) was used to block the hepatic arterial blood supply and simulate TAE.Blood perfusion of tumor in control laparotomy control,and HAL group was analyzed by Hoechst 33342 labeling assay,the serum VEGF level was assayed by ELISA,the expression of VEGF and MMP-1 mRNA was detected by in situ hybridization.RESULTS:Two days after HAL.the number of Hoechst 33 342 labeled cells which represent the blood perfusion of tumor directly and hypoxia of tumor indirectliy in HAL group decreased significantly compared with that in control group (329±29VS 384±19,P<0.01),The serum VEGF level in the HAL group increased significantly as against that of the control group (93ng.L^-1±44ng.L^-1VS55ng.L^-1±19ng.L^-1,P<0.05),The expression of VEGFand MMP-1 mRNA in the tumor tissue of the HAL group increased significantly compared with that of the control and the laparotomy control groups(P<0.05),The blood perfusion data of the tumor represented by the number of Hoechst 33342 labeled cells,showed a good linear inverse correlation with the serum VEGF level(r=-0.606,P<0.05),and the expression of VEGF mRNA in the tumor tissue(r=-0.338,P<0.01).CONCLUSION:Blockage of hepatic arterial blood supply results in decreased blood perfusion and increased expression of metastasis-associated genes VEGF and MMP-1 of transplanted liver cancer in rats .Decreased blood Perfusion and hypoxia may be the major cause of upregulated expression of VEGF. | Wei-Jian Guo Jie Li Wan-Long Ling Wen-Hua Gu Jun-Yan Zhuang,Department of Oncology,Xinhua Hospital of Shanghai Second Medical University,Shanghai 200092,China Yong-Rui Bai,Department of Radiotherapy,Xinhua Hospital of Shanghai Second Medical University,Shanghai 200092,China Wen-Zhu Zhang Yu-Fan Cheng,Department of Pathology,Xinhua Hospital of Shanghai Second Medical University,Shanghai 200092,China | 2002 | World Journal of Gastroenterology2002，8，3： | 13 |
| 18 | Effects of quaternary ammonium chain length on the antibacterial and remineralizing effects of a calcium phosphate nanocomposite显示文摘Composites containing nanoparticles of amorphous calcium phosphate(NACP)remineralize tooth lesions and inhibit caries.A recent study synthesized quaternary ammonium methacrylates(QAMs)with chain lengths(CLs)of 3–18 and determined their effects on a bonding agent.This study aimed to incorporate these QAMs into NACP nanocomposites for the first time to simultaneously endow the material with antibacterial and remineralizing capabilities and to investigate the effects of the CL on the mechanical and biofilm properties.Five QAMs were synthesized:DMAPM(CL3),DMAHM(CL6),DMADDM(CL12),DMAHDM(CL16),and DMAODM(CL18).Each QAM was incorporated into a composite containing 20%NACP and 50%glass fillers.A dental plaque microcosm biofilm model was used to evaluate the antibacterial activity.The flexural strength and elastic modulus of nanocomposites with QAMs matched those of a commercial control composite(n 5 6;P.0.1).Increasing the CL from 3 to 16 greatly enhanced the antibacterial activity of the NACP nanocomposite(P,0.05);further increasing the CL to 18 decreased the antibacterial potency.The NACP nanocomposite with a CL of 16 exhibited biofilm metabolic activity and acid production that were 10-fold lesser than those of the control composite.The NACP nanocomposite with a CL of 16 produced 2-log decreases in the colony-forming units(CFU)of total microorganisms,total streptococci,and mutans streptococci.In conclusion,QAMs with CLs of 3–18 were synthesized and incorporated into an NACP nanocomposite for the first time to simultaneously endow the material with antibacterial and remineralization capabilities.Increasing the CL reduced the metabolic activity and acid production of biofilms and caused a 2-log decrease in CFU without compromising the mechanical properties.Nanocomposites exhibiting strong anti-biofilm activity,remineralization effects,and mechanical properties are promising materials for tooth restorations that inhibit caries. | Ke Zhang Lei Cheng Michael D Weir Yu-Xing Bai Hockin HK Xu | 2016 | International Journal of Oral Science2016，8，1： | 13 |
| 19 | Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction显示文摘AIM To study persistence and replication ofheltitis C virus (HCV) in patients' peripheralblood mononuclear cells (PBMC) cultured invitro.METHODS Epstein-Barr virus (EBV) was usedto transform the hepatitis C virus from a HCVpositive patient to permanent lymphoblastoidcell lines (LCL). Positive and negative HCV RNAstrands of the cultured cells and growth mediawere detected by reverse transcriptase-polymerase chain reaction ( RT-PCR ) eachmonth. Core and NS5 proteins of HCV werefurther tested using immunohistochemical SPmethod and in situ RT-PCR.RESULTS HCV RNA positive strands wereconsistently detected the cultured cells for oneyear. The negative-strand RNA in LCL cells andthe positive-strand RNA in supernatants wereobserved intermittently. Immunohistochemicalresults medicated expression of HCV NS3 and Cproteins in LCL cytoplasm mostly. The positivesignal of PCR product was dark blue and mainlylocalized to the LCL cytoplasm. The RT-PCRsignal was eliminated by overnight RNasedigestion but not DNase digestion.CONCLUSION HCV may exist and remainfunctional in a cultured cell line for a longperiod. | Ji Lin Cheng Bao Ling Liu Yi Zhang Wen Bin Tong Zheng Yan Bai Fang Feng Institute of Hepatology,Peoples Hospital,Medical Center of Beijing University,Beijing 10(X)44,China | 2001 | World Journal of Gastroenterology2001，7，3： | 11 |
| 20 | Accuracy of multi-echo Dixon sequence in quantification of hepatic steatosis in Chinese children and adolescents显示文摘BACKGROUND Nonalcoholic fatty liver disease(NAFLD) is currently the outstanding cause of chronic liver disease in children and adolescents, especially in overweight and obese groups. Liver biopsy is the reference standard to diagnose NAFLD but invasive, thus it is not the best choice in clinical diagnosis and follow-up.Magnetic resonance(MR) is widely used in clinical trials to noninvasively quantify liver fat content in adults and children in foreign countries. While currently, it is rarely used in Chinese children and adolescents. We postulated that quantifying hepatic steatosis by MR could be extended to children and adolescents in China.AIM To investigate the accuracy of MR imaging(MRI) in quantifying liver fat with MR spectroscopy(MRS) as a reference. A secondary goal was to assess the prevalence of NAFLD in overweight and obese Chinese children and adolescents.METHODSThere were 86 children and adolescents enrolled in this study, including 65 overweight and obese children and 21 healthy children. The participants underwent MRI and MRS. MRI and MRS were performed using multi-echo Dixon and HISTO sequences, respectively, to calculate hepatic proton density fat fraction(PDFF). Hepatic steatosis was diagnosed using MRS-PDFF > 5% as the threshold. Spearman's analysis was used to evaluate the correlation between MRI and MRS. The agreement between these two methods was assessed by BlandAltman analysis.RESULTS The MRI-PDFF in the MRS region of interest and the entire liver was 9.9% ±10.3% with a range of 0.3%-39.9%, and 10.6% ± 9.4% with a range of 1.9%-38.9%,respectively. The MRS-PDFF was 9.1% ± 10.0%, with a range of 0.5%-37.8%. The incidence of hepatic steatosis detected by MRS-PDFF was 46.5%(40/86) of all participants, all of whom belonged to the overweight and obese group.Spearman's analysis indicated an excellent correlation between multi-echo Dixon and MRS(r > 0.9, P < 0.01). Bland-Altman analysis also demonstrated a good agreement between these two methods.CONCLUSION Multi-echo Dixon shows an excellent correlation and agreement with MRS in quantifying liver fat content and could be a potential tool to detect hepatic steatosis in Chinese children and adolescents. | Yu-Zhen Zhao Yun-Gen Gan Jian-Li Zhou Jia-Qi Liu Wei-Guo Cao Shu-Mei Cheng Da-Ming Bai Meng-Zhu Wang Fang-Qin Gao Shao-Ming Zhou | 2019 | World Journal of Gastroenterology2019，25，12： | 11 |
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