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| 1 | Effects of lysedEnterococcus faecalis FK-23 on experimental allergic rhinitis in a murine model显示文摘In the current study,we sought to investigate whether lysed Enterococcus faecalis FK-23(LFK),a heat-killed probiotic preparation,attenuated eosinophil influx into the upper airway and had immunomodulatory activity in a murine allergic rhinitis model.Eighteen BALB/c mice were divided into three groups;the ovalbumin(OVA)-sensitized/challenged group,which received saline orally for 6 weeks(OVA group),the OVA-sensitized/challenged group,which received LFK orally for 6 weeks(LFK-fed group),and the non-sensitized group,which received saline for 6 weeks(saline control group).Nasal rubbing and sneezing were monitored during the study.After the final challenge,interleukin(IL)-4,interferon(IFN)-γ,and OVA-specific IgE levels in the sera and splenocyte culture supernatants were determined,eosinophilic infiltrate into the upper airway was quantified,and splenic CD4+CD25+ regulatory T cells(Tregs) were examined by flow cytometry.We found that nasal rubbing was significantly reduced in LFK-fed mice compared to the OVA group on d 27 and 35,and sneezing was significantly inhibited by LFK administration for 35 d.LFK-fed mice had significantly less eosinophil influx into the nasal mucosa than the OVA group.There were no significant differences between the LFK-fed group and OVA group in the serum and splenocyte culture supernatant levels of IL-4,IFN-γ,and OVA-specific IgE.Interestingly,the LFK-fed mice had a significantly greater percentage of splenic CD4+CD25+ Tregs than OVA group.Our results indicate that oral administration of LFK may alleviate nasal symptoms,reduce nasal eosinophilia,and increase the percentage of CD4+CD25+ Tregs in experimental allergic rhinitis. | Luping Zhu Takashi Shimada Ruoxi Chen Meiping Lu Qingzhao Zhang Wenmin Lu Min Yin Tadao Enomoto Lei Cheng | 2012 | The Journal of Biomedical Research2012，26，3： | 11 |
| 2 | Dog sciatic nerve gap repaired by artificial tissue nerve graft显示文摘The feasibility of repairing dog sciatic nerve damage by using a biodegradable artificial tissue nerve graft enriched with neuroregenerating factors is investigated. The artificial nerve graft was implanted to a 30 mm gap of the sciatic nerve damage in 7 dogs. The dogs with the same nerve damage that were repaired by interposition of the autologous nerve or were given no treatment served as control group 1 or 2, respectively. The observations include gross and morphological observations, immune reaction, electrophysiological examination, fluorescence tracing of the neuron formation and the number of the neurons at the experimental sites, etc. Results showed that 6 months after the implantation of the graft, the regenerated nerve repaired the damage of the sciatic nerve without occurrence of rejection and obvious inflammatory reaction in all 7 dogs, and the function of the sciatic nerve recovered with the nerve conduction velocity of (23.91±11.35)m/s. The regenerated neurons and the forming of axon could be observed under an electron microscope. This proves that artificial tissue nerve graft transplantation can bridge the damaged nerve ends and promote the nerve regeneration. | GU Xiaosong, ZHANG Peiyun, WANG Xiaodong, DING Fei, PENG Luping and CHENG Hongbing(Nantong Medical College, The Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong 226001, China) | 2003 | Progress in Natural Science:Materials International2003，13，8： | 6 |
| 3 | Protective effects of Buyinqianzheng Formula on mitochondrial morphology by PINK1/Parkin pathway in SH-SY5Y cells induced by MPP^(+)显示文摘Objective:Buyinqianzheng Formula(BYQZF)is clinically employed in traditional Chinese medicine to treat Parkinson's disease(PD)by improving mitochondrial dysfunction.However,the underlying mechanisms by which BYQZF affects mitochondrial morphology remain unknown.Therefore,we observed the effects of BYQZF on mitochondria from the perspective of the PINK1/Parkin pathway.Methods:Cell survival rates were assessed by Cell Counting Kit-8 assay.Expression levels of PINK1 and Parkin mRNA were examined by qRT-PCR.Protein expression levels of PINK1,PINK1-Ser228,Parkin,Parkin-Ser65,Drp1,and Drp1-Ser637 were examined by western blotting.PINK1,Parkin,and MitoTracker?Red CMXRos(MTR)were stained by triple-labeled immunofluorescence,and observed under laser confocal microscopy.Results:Cell survival rate,mitochondrial form factor,mean length and number of mitochondrial network branches,mitochondrial activity,m RNA expression levels of PINK1 and Parkin,and protein expression levels of PINK1,Parkin,and Drp1-Ser637 were reduced after 1-methyl-4-phenylpyridinium(MPP^(+))intervention.In contrast,Pearson's correlation coefficients between PINK1 and Parkin,and between Parkin and MTR,as well as protein expression levels of PINK1-Ser228,Parkin-Ser65,and Drp1 increased significantly after MPP^(+) intervention.Treatment with BYQZF increased cell survival rate,mitochondrial form factor,mean length and number of mitochondrial network branches,mitochondrial activity,m RNA expression levels of PINK1 and Parkin,and expression of PINK1,Parkin,and Drp1-Ser637 proteins.Pearson's correlation coefficients between PINK1 and Parkin,and between Parkin and MTR,as well as protein expression levels of PINK1-Ser228,Parkin-Ser65,and Drp1 decreased after BYQZF treatment.Conclusion:These results demonstrate that BYQZF has a protective effect on mitochondrial molecular mechanisms in the PD cell model,and the mechanism is related to the PINK1/Parkin pathway. | Haojie Ma Zhenyu Guo Cong Gai Cuicui Cheng Jinkun Zhang Yuxin Zhang Luping Yang Wandi Feng Yushan Gao Hongmei Sun | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，3： | 3 |
| 4 | The progress and prospect of assessment measures of uterine cavity function for infertility patients显示文摘Objective:To explore the progress and prospect of evaluation methods for infertility patients.Methods:From September 2017 to December 2020,199 cases of infertility patients who have accepted mini-hysteroscopy examination in the Department of Gynecological Endocrinology,Beijing Obstetrics and Gynecology Hospital,Capital Medical University.The patients who had primary ovary insufficiency,tubal infertility were excluded;patients with hysteroscopy contradiction,such as acute or sub-acute vaginal tract inflammation,endometrium lesion or endometrium carcinoma,severe cardiovascular,liver or kidney insufficiency were excluded.40 patients were excluded and 15 patients lost follow up,144 patients were eventually included in the analysis.The patient’s age,body mass index,infertility etiology,preoperative and postoperative stimulate ovulation cycle,pregnancy state and pregnancy time were recorded.The effect of mini-hysteroscopy on ovulation induction cycle was analyzed in pregnant patients.Results:The average age of 144 patients was 32.10±4.67 years old.96(66.67%)patients were found to have intrauterine abnormalities,while 48(33.33%)patients were found to have normal intrauterine cavity.48 cases of pregnancy,accounting for 33.33%;the median and interquartile ranges[M(Q1,Q3)]of ovulation induction cycles before and after surgery were respectively[3(0,5)],[2(1,3)],the difference was statistically significant(P<0.05).Among the 48 pregnant patients,32 of them had no structural lesions in hysteroscopy,the ovulation induction cycle of these patients before and after hysteroscopy were respectively[5(0,9)],[1(0,3)],the difference was statistically significant(P<0.05).Conclusion:Uterine cavity evaluation is important for the assessments of fertility.As an advanced diagnosis instrument,mini-hysteroscopy can not only timely detect the intrauterine abnormalities of patients,hut also with great application value in shorten the ovulation cycle in infertility patients and increase the pregnancy rate. | Luping Zhang Rui Ju Xiangyan Ruan Yinmei Dai Husheng Wang Fengyu Jin Jing Jin Muqing Gu Jiaojiao Cheng Yu Yang Xin Xu Thomas Roemer | 2021 | Global Health Journal2021，5，2： | 3 |
| 5 | Analysis of an example of the application of baclofen in the clini- cal treatment of tetanus显示文摘 | Huo Lingbo Shen Luping Cheng Huimin | 2013 | Chinese Journal of Clinicians (Electronic Version)2013，，3： | 1 |
| 6 | The impact of spatial mismatch on residents in low-income housing neighbourhoods: a study of the Guangzhou metropolis, China 显示文摘 | ZHOU Suhong WU Zhidong CHENG Luping | 2013 | Urban Studies2013，50，9： | 1 |
| 7 | Annual Variation of Water Quality in Aquaculture Ponds for Apostichopus japonicus显示文摘By measuring water temperature,salinity,pH,dissolved oxygen,nitrite and ammonia nitrogen,the annual variation regularity of water quality in the aquaculture ponds for sea cucumbers Apostichopus japonicus in Dalian were studied.Results showed that the physicochemical indicators kept changing in the year,and the various ranges of water temperature,salinity,pH,dissolved oxygen,nitrite,and ammonia nitrogen were 6.38-27.27℃,29.20‰-33.78‰,7.86-8.38,5.19-13.36 mg/L,0.0037-0.0090 mg/L and 0.0171-0.0908 mg/L,respectively.Analysis indicated that there was an extremely significant negative correlation between water temperature and dissolved oxygen(P<0.01),an extremely significant positive correlation between water temperature and ammonia nitrogen(P<0.01),a significant negative correlation between salinity and nitrite(P<0.05),a significant negative correlation between dissolved oxygen and nitrite(P<0.05),and an extremely significant negative correlation between dissolved oxygen and ammonia nitrogen(P<0.01)in the aquaculture ponds for sea cucumber in Dalian.Research showed that the water quality of aquaculture ponds kept fluctuating all the time during the tested period,there were complicated and close relationships among physicochemical indicators,and a slight variation of external factors would bring consecutive reactions to different indicators in the water quality of aquaculture ponds.A reference for the production and management of sea cucumbers could be provided from the results. | Yaoming LUO Yuan CHENG Duohui LI Zhiying GAO Luping WANG Jiangyi WU Dawei JIANG | 2019 | Asian Agricultural Research2019，11，11： | 1 |
| 8 | Fruit stem blight on kiwifruit (Actinidia chinensis) in China显示文摘 | Cheng Li Junxi Jiang Luping Liu Chaoyu Cui Ting Huang Dengquan Liu | 2013 | Canadian Journal of Plant Pathology2013，，3： | 1 |
| 9 | Multi-grained system integration for hybrid-paradigm brain-inspired computing显示文摘Hybrid neuromorphic computing supporting the prevailing artificial neural networks and neuroscience-inspired models/algorithms offers substantial flexibility for cross-paradigm model integration.It is one of the most promising technologies for accelerating intelligence development,ultimately contributing to artificial general intelligence development.Recently,an increasing number of hybrid neuromorphic computing chips have been reported,but such research focuses on chip design without demonstrating systems for large-scale workloads.To this end,we construct a multi-grained system based on many Tianjic chips,presenting a large-scale system for hybrid-paradigm brain-inspired computing.With different numbers of chips and different connection topologies,we develop a Tianjic card and a Tianjic board as the infrastructure for building embedded systems and cloud servers,respectively.Extensive measurements of the communication latency,computational latency,and power consumption evidence the superior potential of Tianjic systems for exploring brain-inspired computing for artificial general intelligence. | Jing PEI Lei DENG Cheng MA Xue LIU Luping SHI | 2023 | Science China(Information Sciences)2023，66，4： | 1 |
| 10 | The construction of OPA1 overexpression plasmid and the cell model显示文摘The purpose of this study is to construct OPA1 overexpression plasmid and transfected into human neuroblastoma SH-SY5Y cells to test the overexpression efficiency.Human neuroblastoma SH SY5Y cells were transfected with the OPA1 overexpression plasmid by Lipo3000 transfection reagent in liposome transduction technology.A total of 3 groups were set up:the control group,negative control group,and OPA1 overexpression model group.RT-PCR technique was used to detect the expression of OPA1 at mRNA level.In addition,Western blot technique was used to detect the expression of OPA1 at protein level.We found that the mRNA and protein expression of OPA1 were significantly increased after transfection with OPA1 overexpression plasmid.These results highlight using liposome transduction technology,the OPA1 overexpression plasmid was successfully transfected into SH-SY5Y cells by Lipo3000 and the cell model of OPA1 gene overexpression was successully established. | Ma Haojie Gai Cong Feng Wandi Cheng Cuicui Zhou Mengqi Zhang Jinkun Zhang Yuxin Yang Luping Guo Zhenyu Sun Hongmei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 11 | Effect of Timosaponin B-Ⅱ on morphology and function of mitochondria in PD cell model显示文摘Explore the effect of Timosaponin B-Ⅱ on morphology and function of mitochondria in Parkinson's disease(PD)cell model,and lay the foundation for screening and mechanism research of anti-Parkinson's disease drugs.The PD cell model was constructed by applying MPP+to SH-SY5Y cells for 48 h,and they were treated with low,.medium and high concentrations of Timosaponin B-Ⅱ. | Zhou Mengqi Gai Cong Feng Wandi Ma Haojie Cheng Cuicui Zhao Haihong Zhang Yuxin Zhang Jinkun Yang Luping Sun Hongmei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 12 | Electroacupuncture treatment attenuates stress-induced depressive-like behavior in rats through modulation of PNNs in the mPFC显示文摘The perineuronal nets(PNNs)are a kind of extracellular matrix structure which mainly surround parvalbumin-positive(PV^(+))GABAergic interneurons.The aim of this study is to investigate the role of PNNs in the medial prefrontal cortex(mPFC)in the antidepressant effect of electroacupuncture treatment.Adult rats underwent chronic unpredictable mild stress(CUMS)for 28 days,and electroacupuncture treatment of Baihui and Yintang acupoints was administrated from day 8 to day 28.Then we detected the depressive-and anxiety like behavior of rats,the density of PNNs,the percentage of PV^(+)neurons that surround PNNs,the expression levels of PNNs constituting proteins in the mPFC. | Zhang Yuxcin Hu Die Gai Cong Ma Haojie Cheng Cuicui Zhang Jinkun Yang Luping Sun Hongmei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 13 | Effects of Buyang Huanwu decoction and its disassembled prescriptions on neurological function and angiogenesis in rats with ischemia stroke显示文摘This study was performed to investigate the effect and mechanism of Buyang Huanwu Decoction(BYHWD)and its disassembled prescriptions on nerve function and angiogenesis in ischemic stroke rats with Qi deficiency and blood stasis syndrome.Rats were randomly divided into six groups:Sham group,Model group,BYHWD group,Qi-tonifying group,Blood-activating group and Butyphthalide group.Rats were used to make permanent middle cerebral artery occlusion(MCAO)after 10 days of simulated intervention with compound factors of Qi deficiency and blood stasis syndrome,followed by the medicine group was administered intragastrically for a week. | Yang Luping Gai Cong Ma Haojie Cheng Cuicui Zhang Jinkun Zhang Yuxin Sun Hongmei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 14 | Ore-forming fluid characteristics and ore-forming materials source of the Tudimiaogou – Yindongshan lead-zinc polymetallic orefield, west Henan显示文摘1 Introduction The Tudimiaogou-Yindongshan lead-zinc polymetallic orefield is located in the Tudimiaogou-Weimoshi lead and zinc silver polymetallic metallogenic belt.The belt is an important part of southwestern Henan lead and | TAN Huajie SHAO Yongjun ZHENG Minghong ZHOU Houdi ZHANG Jiandong PENG Guangxiong Cheng Luping | 2017 | Acta Geologica Sinica(English Edition)2017，91，S1： | 0 |
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