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445篇 您的检索式:作者名="Wang Yaping"
    题名 作者 年代 出处 被引量
1Expanding the Range of CRISPR/Cas9 Genome Editing in Rice显示文摘Xixun Hu Chun Wang Yaping FU Qing Liu Xiaozhen Jiao Kejian Wang 2016Molecular Plant2016,9,6:31
25-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu 2017Cell Research2017,27,10:30
3Genetic analysis of 'all-fish' growth hormone gene transferred carp (Cyprinus carpio L.) and its F_1 generation显示文摘Recornbinant 'all-fish' growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant 'fast-growing' effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the FI generation were genetically analyzed, which gave solid evidence for the hypothesis that 2-3 chromosomes are integrated with trans-gene. In addition, the distinct biological effects for multi-site-integrated transgenes were further discussed. The present study opens a door for the breeding of 'fast-growing' transgenic fish.WANG Yaping, HU Wei, WU Gang,SUN Yonghua, CHEN Shangping, ZHANG Fuying,ZHU Zuoyan, FENG Jianxin & ZHANG Xirui1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2. Henan Institute of Aquaculture, Zhengzhou 450044, China 2001Chinese Science Bulletin2001,46,14:23
4A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice显示文摘Generating mutants bearing multiple gene modifications is essential for determining the functions of gene family members with redundant functions,or for analyzing epistatic relationships in genetic pathways.Using conventional methods,mutants with multiple gene mutations are generated by several rounds of intercrossing plants carrying a single mutation and identification of the offspring.This process is both time-Chun Wang Lan Shen Yaping Fu Changjie Yan Kejian Wang 2015Journal of Genetics and Genomics2015,42,12:22
5QTL editing confers opposing yield performance in different rice varieties显示文摘Grain yield is one of the most important and complex trait for genetic improvement in crops; it is known to be controlled by a number of genes known as quantitative trait loci(QTLs). In the past decade, many yield-contributing QTLs have been identified in crops.However, it remains unclear whether those QTLs confer the same yield performance in different genetic backgrounds. Here, we performed CRISPR/Cas_9-mediated QTL editing in five widely-cultivated rice varieties and revealed that the same QTL can have diverse, even opposing, effects on grain yield in different genetic backgrounds.Lan Shen Chun Wang Yaping Fu Junjie Wang Qing Liu Xiaoming Zhang Changjie Yan Qian Qian Kejian Wang 2018Journal of Integrative Plant Biology2018,60,2:18
6Progress in the evaluation of transgenic fish for possible ecological risk and its containment strategies显示文摘Genetically improved transgenic fish possess many beneficial economic traits; however, the commer-cial aquaculture of transgenic fish has not been performed till date. One of the major reasons for this is the possible ecological risk associated with the escape or release of the transgenic fish. Using a growth hormone transgenic fish with rapid growth characteristics as a subject, this paper analyzes the following: the essence of the potential ecological risks posed by transgenic fish; ecological risk in the current situation due to transgenic fish via one-factor phenotypic and fitness analysis, and mathe-matical model deduction. Then, it expounds new ideas and the latest findings using an artificially simulated ecosystem for the evaluation of the ecological risks posed by transgenic fish. Further, the study comments on the strategies and principles of controlling these ecological risks by using a trip-loid approach. Based on these results, we propose that ecological risk evaluation and prevention strategies are indispensable important components and should be accompanied with breeding re-search in order to provide enlightments for transgenic fish breeding, evaluation of the ecological risks posed by transgenic fish, and development of containment strategies against the risks.HU Wei WANG YaPing ZHU ZuoYan 2007Science China(Life Sciences)2007,50,5:17
7High-resolution data collection for analysis of sediment dynamic processes associatedwith combined current-wave action over intertidal flats显示文摘An MIDAS-400 Customised Data Ac- quisition System was deployed to obtain current ve- locity and suspended sediment concentration profiles over the intertidal flat at Wanggang, on the Jiangsu coast (China). At the same time, seawater tempera- ture, conductivity and pressure were measured. Us- ing a data sampling rate of 4 Hz, the high-frequency pressure signals in response to water surface fluc- tuations were recorded and subsequently trans- formed into wave parameters. The analytical results show that the apparent bed roughness length has a magnitude close to the height of sand ripples, which is much larger than the grain size diameter. The bot- tom shear stress associated with current-wave in- teraction is higher than the tidally-induced stress and intensified turbulent mixing and resuspension. Fur- thermore, several suspended sediment concentration peaks occurred during a tidal cycle, which can be related to strong turbulence near the bed caused by frontal tidal currents and the enhanced resuspension; advection and extreme weather conditions (e. g. storms and strong winds) also played an important role. Finally, large net suspended sediment fluxes were observed to present when the flood or ebb currents prolonged, i.e. the flow became quasi-unid- irectional during a tidal cycle. In general, the MIDAS- 400 is a suitable tool for high-resolution data collec- tion for the situations of combined current-wave ac- tion over intertidal flats; the data obtained can be used to analyze the processes and mechanisms of material transport.WANG Yaping GAO Shu JIA Jianjun 2006Chinese Science Bulletin2006,51,7:17
8Targeted mutagenesis in rice using CRISPR-Cpf1 system显示文摘Cpfl is a class 2/type V CRISPR effector that has been recently harnessed for genome editing(Zetsche et al.,2015;Hur et al.,2016;Kim et al.,2016).Cpfl recognizes thymidine-rich sequence as the protospacer-adjacent motif(PAM)at the 5'end of target sequences.In addition,Cpfl requires only a single shorter crRNAXixun Hu Chun Wang Qing Liu Yaping Fu Kejian Wang 2017Journal of Genetics and Genomics2017,44,1:17
9Economic Dispatch of Power Systems with Virtual Power Plant Based Interval Optimization Method显示文摘Load prediction and power prediction uncertainties are inevitable aspects of a virtual power plant(VPP).In power system economic dispatch(ED)modeling,the interval is used to describe prediction uncertainties.An ED model with interval uncertainty is established in this paper.The probability degree definition is adopted to convert the interval-based economic dispatch model into a deterministic model for the purposes of solving the modeling problem.Simulation tests are performed on a 10-machine system using professional optimization software(LINGO).The simulation results verify the validity of the proposed interval-based scheme for the economic dispatch of a power system with VPP.Chongxin Huang Dong Yue Jun Xie Yaping Li Ke Wang 2016CSEE Journal of Power and Energy Systems2016,2,1:17
10Rapid growth cost in “all-fish” growth hormone gene transgenic carp:Reduced critical swimming speed显示文摘Evidence has accumulated that there is a trade-off between benefits and costs associated with rapid growth. A trade-off between growth rates and critical swimming speed (Ucrit) had been also reported to be common in teleost fish. We hypothesize that growth acceleration in the F3 generation of 'all-fish' growth hormone gene (GH) transgenic common carp (Cyprinus carpio L.) would reduce the swimming abilities. Growth and swimming performance between transgenic fish and non-transgenic controls were compared. The results showed that transgenic fish had a mean body weight 1.4―1.9-fold heavier,and a mean specific growth rate (SGR) value 6%―10% higher than the controls. Transgenic fish,however,had a mean absolute Ucrit (cm/s) value 22% or mean relative Ucrit (BL/s) value 24% lower than the controls. It suggested that fast-growing 'all-fish' GH-transgenic carp were inferior swimmers. It is also supported that there was a trade-off between growth rates and swimming performance,i.e. faster-growing individuals had lower critical swimming speed.LI DeLiang FU CuiZhang HU Wei ZHONG Shan WANG YaPing ZHU ZuoYan 2007Chinese Science Bulletin2007,52,11:14
11The application of geostatistics in grain size trend analysis: A case study of eastern Beibu Gulf显示文摘有 71 件表面沉积样品,从东方 Beibu 海湾收集。片刻参数(即吝啬的尺寸,排序并且偏斜度) 在使用谷物尺寸分析以后被获得。geostatistical 分析然后被使用为这些参数学习空间自相关;当时范围,在 semivariogram 的一个参数米空间自相关的规模,被估计。结果显示为排序系数的范围身体上是有意义的。从谷物尺寸趋势分析模型计算的趋势向量根据以前的研究与年度海洋发行量模式和沉积运输率一致。因此,从吝啬的尺寸的 semivariogram 导出的范围能在谷物尺寸趋势分析被用作典型距离,它可以移开基于经验或严峻的方法得到典型距离的传统的方法引起的偏爱。因此从分析能也在未来为沉积采样密度的决心为有用信息提供的 geostatistical 的结果回答工作。MA Fei WANG Yaping LI Yan YE Changjiang XU Zhiwei ZHANG Fan 2010Journal of Geographical Sciences2010,20,1:14
12Aquaporin-dependent excessive intrauterine fluid accumulation is a major contributor in hyper-estrogen induced aberrant embryo implantation显示文摘Ying Zhang Qi Chen He Zhang Qiang Wang Rong Li Yaping Jin Haibin Wang Tonghui Ma Jie Qiao Enkui Duan 2015Cell Research2015,25,1:14
13Myelin Oligodendrocyte Glycoprotein-IgG Contributes to Oligodendrocytopathy in the Presence of Complement, Distinct from Astrocytopathy Induced by AQP4-IgG显示文摘Immunoglobulin G against myelin oligodendrocyte glycoprotein(MOG-Ig G) is detectable in neuromyelitis optica spectrum disorder(NMOSD) without aquaporin-4 Ig G(AQP4-Ig G), but its pathogenicity remains unclear.In this study, we explored the pathogenic mechanisms of MOG-Ig G in vitro and in vivo and compared them with those of AQP4-Ig G. MOG-Ig G-positive serum induced complement activation and cell death in human embryonic kidney(HEK)-293 T cells transfected with human MOG. In C57 BL/6 mice and Sprague-Dawley rats, MOG-Ig G only caused lesions in the presence of complement. Interestingly, AQP4-Ig G induced astroglial damage, while MOGIg G mainly caused myelin loss. MOG-Ig G also induced astrocyte damage in mouse brains in the presence ofcomplement. Importantly, we also observed ultrastructural changes induced by MOG-Ig G and AQP4-Ig G. These findings suggest that MOG-Ig G directly mediates cell death by activating complement in vitro and producing NMOSDlike lesions in vivo. AQP4-Ig G directly targets astrocytes,while MOG-Ig G mainly damages oligodendrocytes.Ling Fang Xinmei Kang Zhen Wang Shisi Wang Jingqi Wang Yifan Zhou Chen Chen Xiaobo Sun Yaping Yan Allan G. Kermode Lisheng Peng Wei Qiu 2019Neuroscience Bulletin2019,35,5:13
14Necklace-like Si@C nano?bers as robust anode materials for high performance lithium ion batteries显示文摘Silicon is believed to be a promising anode material for lithium ion batteries because of its highest theoretical capacity and low discharge potential. However, severe pulverization and capacity fading caused by huge volume change during cycling limits its practical application. In this work, necklace-like N-doped carbon wrapped mesoporous Si nanofibers(NL-Si@C) network has been synthesized via electrospinning method followed by magnesiothermic reduction reaction process to suppress these issues. The mesoporous Si nanospheres are wrapped with N-doped carbon shells network to form yolk-shell structure.Interestingly, the distance of adjacent Si@C nanospheres can be controllably adjusted by different addition amounts of SiO_2 nanospheres. When used as an anode material for lithium ion batteries, the NL-Si@C-0.5 exhibits best cycling stability and rate capability. The excellent electrochemical performances can be ascribed to the necklace-like network structure and N-doped carbon layers, which can ensure fast ions and electrons transportation, facilitate the electrolyte penetration and provide finite voids to allow large volume expansion of inner Si nanoparticles. Moreover, the protective carbon layers are also beneficial to the formation of stable solid electrolyte interface film.Xiangzhong Kong Yuchao Zheng Yaping Wang Shuquan Liang Guozhong Cao Anqiang Pan 2019Science Bulletin2019,64,4:13
15Phosphorus oxoanion-intercalated layered double hydroxides for high-performance oxygen evolution显示文摘合理设计和为氧进化反应(OER ) 的有效、划算的电极的控制制造为探讨崭新的精力危机是批评的。有特定的夹层阴离子的镍铁分层的双氢氧化物(NiFe-LDHs )( 即磷酸盐,亚磷酸盐,和次磷酸盐) 被一个一起沉淀方法制作并且作为氧进化 electrocatalysts 调查了。磷 oxoanion 的置闰在一个碱的答案提高了 OER 活动;最佳的表演(即, 215 mV, 37.7 mV/dec 的一个小 Tafel 斜坡,和稳定的电气化学的行为的一个低发作潜力) 与设置次磷酸盐的 NiFe-LDH 催化剂被完成,在传统的设置碳酸盐的 NiFe-LDH 上的示威戏剧的改进以活动和耐久性。这提高的表演被归因于在设置的磷的 oxoanions 和分享边的瞬间 6 之间的相互作用(M = Ni, Fe ) 层,它修改表面 Ni 地点的电子结构。这个概念应该为更有效的基于 LDH 的氧进化 electrocatalysts 的设计是鼓舞人心的。Ma Luo Zhao Cai Cheng Wang Yongmin Bi Li Qian Yongchao Hao Li Li Yun Kuang Yaping Li Xiaodong Lei Ziyang HUO Wen Liu Hailiang Wang Xiaoming Sun Xue Duan 2017Nano Research2017,10,5:12
16The impact of climate change and human activities on streamflow and sediment load in the Pearl River basin显示文摘This paper uses monthly streamflow, suspended sediment concentration, and meteorological data to examine the impact of human activity and climate change on streamflow and sediment load in the Pearl River basin from the 1950s to the 2000s. The influences of climate change and human activities on hydrological processes were quantitatively evaluated using the Mann–Kendall abrupt change test and power rating curves. The results showed that:(1) abrupt changes and turning points in streamflow occurred in 1963, 1983, and 1991 which were found to be consistent with global ENSO events and volcanic eruptions. However, abrupt changes in sediment load showed significant spatial differences across the Pearl River basin. For the Xijiang River, an abrupt change in sediment load occurred in 2002, and after 2007 the change becomes significant at the 95% confidence level. At Beijiang and Dongjiang, abrupt changes in sediment load occurred in 1998 and 1988, respectively.(2) The time series of sediment load data was divided into four periods according to abrupt changes. The contribution of climate change and human activities is different in the different rivers. For the Xijiang River, compared with the first period, climate change and human activities contributed 83% and 17%, respectively, to the increasing sediment load during the second period. In the third period, the variation of sediment load followed a decreasing trend. The contribution from climate change and human activities also changed to t236% and -136%, respectively. In the fourth period, climate change and human activities contributed -32% and t132%, respectively. Meanwhile, For the Beijiang River, climate change and human activities contributed 90% and 10% in the second period, the contribution of climate change increased to t115% and human activities decreased to -15% in the third period. In the fourth period, the value for climate change decreased to t36% and human activities increased to t64%. For the Dongjiang River, the contribution of human activities was from 74.5% to 90%, and the values for climate change were from 11% to 25%. Therefore, the effect of human activity showed both spatial and temporal differences, and it seems likely that the decreased sediment load will continue to be controlled mainly by human activities in the future.Chuangshou Wu Changchen Ji Benwei Shi Yaping Wang Jianhua Gao Yang Yang Jinbin Mu 2019International Journal of Sediment Research2019,34,4:12
17Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza.Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen 2022Science China(Life Sciences)2022,65,4:12
18Rapid growth and sterility of growth hormone gene transgenic triploid carp显示文摘Triploid carp(100%)with 150(3n=150)chromosomes were obtained by crossing the females of improved tetraploid hybrids(♀, 4n=200)of red crucian carp(♀)×common carp(♂)with the males of diploid yellow river carp(♂,2n=100).The crosses yielded transgenic triploid carp(positive triploid fish,44.2%of the progeny)and non-transgenic triploid carp(negative triploid fish). Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season.Morphologically,both the positive and negative triploid fish were similar.They had a spindle-shaped,laterally compressed,steel grey body with two pairs of barbells.Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents.The positive and negative triploid fish were raised in the same pond for 2 years.The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish.The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish.Thus,we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny.YU Fan XIAO Jun LIANG XiangYang LIU ShaoJun ZHOU GongJian LUO KaiKun LIU Yun HU Wei WANG YaPing ZHU ZuoYan 2011Chinese Science Bulletin2011,56,16:11
19Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing显示文摘The regulation of stomatal lineage cell development has been extensively investigated.However,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been reported.In this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis seedlings.We identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker genes.Comparative analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother cells.Examination of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these genes.Collectively,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development.Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing Sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo Jean-David Rochaix Yuchen Miao Xuwu Sun 2020Molecular Plant2020,13,8:11
20Sedimentation and morphological changes at Yuantuojiao Point, estuary of the North Branch, Changjiang River显示文摘The North Branch, separated by the Chongming Island, was once the main channel in the estuary of the Changjiang River. Reclamation and a decrease in runoff to the North Branch had led to the narrowing and shallowing of the channel. The Yuantuojiao Point is located at the intersecting point connecting the North Branch of the Changjiang River and the Jiangsu coastline. Erosion cliffs are developed between the typical silty-muddy tidal flat and the salt marsh occupied by Spartina alterniflorea, and this has changed rapidly over the past few years. The sediment grain size analysis results of the surficial and two core samples indicate that the Yuantuojiao Point tidal flat experienced continuous accretional processes. Based upon 137 Cs analysis results of the YT and YY Cores sampled from the tidal flat at the Yuantuojiao Point, the average sedimentation rate of the YT Core was 2.30 cm/a from 1963 to 2007, and 2.38 cm/a from 1954 to 2007 for the YY Core. The sedimentation rates of both core locations have declined since the 1960s corresponding to the seaward reclamation at the Yuantuojiao Point. The average sedimentation rates at the Yuantuojiao Point were similar to that of the silty-muddy tidal flat at the northern Jiangsu coast, but lower than that of the south of the Changjiang River Estuary. According to field morphological investigations from 2006 to 2008 on the salt marsh at the Yuantuojiao Point, cliffs retreated markedly by storm surges and disappeared gradually because of the rapid sedimentation on the silty-muddy tidal flat. The maximum annual retreat reached 10 m. The recent sedimentation and morphological changes of the Yuantuojiao Point tidal flat not only displayed the retreat of the salt marsh and the disappearance of cliffs, but also was accompanied by rapid sedimentation of the silty-muddy tidal flat and the salt marsh, indicating the responses to the tidal currents, storm surges, Spartina alterniflorea trapping sediments and large-scale reclamation. The sediment grain size and their trends, southward coastal flow, and sandspits of the longshore bars suggest that the main sediment source at the Yuantuoijao Point, estuary of the North Branch was possibly from the Changjiang River before 1958, since then, it has been from the south of the submarine radial sand ridges of the southern Huanghai Sea (Yellow Sea).XIE Li ZHANG Zhenke ZHANG Yunfeng WANG Yaping HUANG Xianjin 2013Acta Oceanologica Sinica2013,32,2:11
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