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| 1 | Antisense MMP-9 RNA inhibits malignant glioma cell growth in vitro and in vivo显示文摘The matrix-degrading metalloproteinases (MMPs), particularly MMP-9, play important roles in the pathogenesis and development of malignant gliomas. In the present study, the oncogenic role of MMP-9 in malignant glioma cells was investigated via antisense RNA blockade in vitro and in vivo. TJ905 malignant glioma cells were transfected with pcDNA3.0 vector expressing antisense MMP-9 RNA (pcDNA-AS-MMP9), which significantly decreased MMP-9 expression, and cell proliferation was assessed. For in vivo studies, U251 cells, a human malignant glioma cell line, were implanted subcutaneously into 4-to 6-week-old BALB/c nude mice. The mice bearing well-established U251 gliomas were treated with intratumoral pcDNA-AS-MMP9-Lipofectamine complex (AS-MMP-9-treated group), subcutaneous injection of endostatin (endostatin-treated group), or both (combined therapy group). Mice treated with pcDNA (empty vector)-Lipofectamine served as the control group. Four or eight weeks later, the volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity were assayed. We demonstrate that pcDNA-AS-MMP9 significantly decreased MMP-9 expression and inhibited glioma cell proliferation. Volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity in the antisense-MMP-9-treated and therapeutic alliance groups were significantly lower than those in the control group. The results suggest that MMP-9 not only promotes malignant glioma cell invasiveness, but also affects tumor cell proliferation. Blocking the expression of MMP-9 with antisense RNA substantially suppresses the malignant phenotype of glioma cells, and thus can be used as an effective therapeutic strategy for malignant gliomas. | Cuiyun Sun Qian Wang Hongxu Zhou Shizhu Yu Alain R. Simard Chunsheng Kang Yanyan Li Yanling Kong Tongling An Yanjun Wen Fudong Shi Junwei Hao | 2013 | Neuroscience Bulletin2013，29，1： | 14 |
| 2 | Propofol differentially induces unconsciousness and respiratory depression through distinct interactions between GABAa receptor and GABAergic neuron in corresponding nuclei显示文摘Propofol is the most commonly used intravenous anesthetic worldwide.It can induce loss of consciousness prior to the occurrence of severe respiratory suppression,which is also a pharmacodynamic feature of all general anesthetics.However,the neural mechanisms underlying this natural phenomenon are controversial and highly related to patient safety.In the present study,we demonstrated that the pharmacodynamic effects of propofol(50 and 100 μM)on suppression of consciousness-related excitatory postsynaptic currents in the medial prefrontal cortex(mPFC)and centromedian nucleus of the thalamus(CMT)were lower than those in the kernel respiratory rhythmogenesis nucleus pre-Botzinger complex(PrBo).Furthermore,we unexpectedly found that the GABAa receptor β3 subunit is the key target for propofol's action and that it is mutually and exclusively expressed in GABAergic neurons.It is also more abundant in the mPFC and CMT,but mainly co-localized with GABAergic neurons in the PrBo.As a result,the differentiated expression pattern should mediate more neuron suppression through the activation of GABAergic neurons in the mPFC and CMT at low doses of propofol(50 μM).However,PrBo GABAergic neurons were only activated by propofol at a high dose(100 μM).These results highlight the detailed pharmacodynamic effects of propofol on consciousness-related and respiration-related nuclei and provide the distinct interaction mechanism between the β3 subunit and GABAergic neurons in mediating the suppression of consciousness compared to the inhibition of respiration. | Junli Jiang Yingfu Jiao Po Gao Wen Yin Wei Zhou Yunchun Zhang Yanjun Liu Daxiang Wen Yuan Wang Liang Zhou Tian Yu Weifeng Yu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，8： | 11 |
| 3 | Twa1/Gid8 is a β-catenin nuclear retention factor in Wnt signaling and colorectal tumorigenesis显示文摘 | Yi Lu Shanshan Xie Wen Zhang Cheng Zhang Cheng Gao Qiang Sun Yuqi Cai Zhangqi Xu Min Xiao Yanjun Xu Xiao Huang Ximei Wu Wei Liu Fudi Wang Yibin Kang Tianhua Zhou | 2017 | Cell Research2017，27，12： | 9 |
| 4 | Impacts of intraseasonal oscillation on the onset and interannual variation of the Indian summer monsoon显示文摘The role of the intraseasonal oscillation (ISO) on the seasonal and interannual variations of the Indian summer monsoon is investigated based on the analysis of observational data. It is shown that the ISO significantly contributes to the establishment of low-level westerlies during the monsoon onset and developing periods. The effect of the ISO on the annual cycle of the monsoon is through nonlinear eddy momentum transport. On the interannual timescale, the Indian summer monsoon rainfall exhibits a significant out-of-phase relationship with the ISO intensity over the Indian monsoon region. In strong ISO years it appears the weak monsoon when there is an abnormal high over the India subcontinent in the lower troposphere. In weak ISO years there exists an abnormal low and the strong monsoon ap-pears. | QI YanJun ZHANG RenHe LI Tim WEN Min | 2009 | Chinese Science Bulletin2009，54，5： | 6 |
| 5 | Development characteristics of the fault system and its control on basin structure, Bodong Sag, East China显示文摘The Bodong Sag,located in the Bohai Sea,offshore China,is one of the most petroliferous basins in China.Based on three dimensional seismic reflection data and time slice data,we analyze the fault system of the Bodong area in detail,establish the fault structure pattern of different types and summarize the distribution of the fault system.It is concluded that the development characteristics of the Cenozoic fault system are in accordance with the dextral stress field of the Tanlu Fault,which displayed a brush structure with NNE strike-slip faults as its principal faults,NE-trending extensional faults as secondary faults and EW-trending faults as minor faults.Faults can be divided into (1) strike-slip type,(2) extensional type,(3) strike-slip extensional type and (4) extensional strike-slip type.The spatial structures of different faults have obvious differences because of the fault properties and activity intensity.The fault system at different stages shows tremendous differences because of the transition of the Tanlu Fault from sinistral strike-slip to dextral strike-slip,the transition between extension and strike-slip,and the transition from mantle upwelling to thermal subsidence.According to the controlling effect of faults on basin structure,the Cenozoic basin experienced four evolutionary stages,(a) transition stage from sinistral strike-slip to dextral strike-slip,(b) strike-slip extensional faulted stage,(c) extensional strike-slip faulted stage and (d) strike-slip depression stage.The identification of temporal and spatial differences of faults could be used as a significant guideline for oil and gas exploration in the Bodong area. | Wu Zhiping Cheng Yanjun Yan Shiyong Su Wen Wang Xin Xu Changgui Zhou Xinhuai | 2013 | Petroleum Science2013，10，4： | 4 |
| 6 | Thymosin Alpha-1 Inhibits Complete Freund’s Adjuvant-Induced Pain and Production of Microglia-Mediated Pro-inflammatory Cytokines in Spinal Cord显示文摘Activation of inflammatory responses regulates the transmission of pain pathways through an integrated network in the peripheral and central nervous systems.The immunopotentiator thymosin alpha-1(Tal)has recently been reported to have anti-inflammatory and neuroprotective functions in rodents.However,how Tα1 affects inflammatory pain remains unclear.In the present study,intraperitoneal injection of Tal attenuated complete Freund's adjuvant(CFA)-induced pain hypersensitivity,and decreased the up-regulation of pro-inflammatory cytokines(TNF-α,IL-1β,and IL-6)in inflamed skin and the spinal cord.We found that CFA-induced peripheral inflammation evoked strong microglial activation,but the effect was reversed by Tα1.Notably,Tα1 reversed the CFA-induced up-regulation of vesicular glutamate transporter(VGLUT)and down-regulated the vesicular γ-aminobutyric acid transporter(VGAT)in the spinal cord.Taken together,these results suggest that Tα1 plays a therapeutic role in inflammatory pain and in the modulation of microgliainduced pro-inflammatory cytokine production in addition to mediation of VGLUT and VGAT expression in the spinal cord. | Yunlong Xu Yanjun Jiang Lin Wang Jiahua Huang Junmao Wen Hang Lv Xiaoli Wu Chaofan Wan Chuanxin Yu Wenjie Zhang Jiaying Zhao Yinqi Zhou Yongjun Chen | 2019 | Neuroscience Bulletin2019，35，4： | 4 |
| 7 | Local inhibition of GABA affects precedence effect in the inferior colliculus显示文摘The precedence effect is a prerequisite for faithful sound localization in a complex auditory environment,and is a physiological phenomenon in which the auditory system selectively suppresses the directional information from echoes.Here we investigated how neurons in the inferior colliculus respond to the paired sounds that produce precedence-effect illusions,and whether their firing behavior can be modulated through inhibition with gamma-aminobutyric acid(GABA).We recorded extracellularly from 36 neurons in rat inferior colliculus under three conditions: no injection,injection with saline,and injection with gamma-aminobutyric acid.The paired sounds that produced precedence effects were two identical 4-ms noise bursts,which were delivered contralaterally or ipsilaterally to the recording site.The normalized neural responses were measured as a function of different inter-stimulus delays and half-maximal interstimulus delays were acquired.Neuronal responses to the lagging sounds were weak when the inter-stimulus delay was short,but increased gradually as the delay was lengthened.Saline injection produced no changes in neural responses,but after local gamma-aminobutyric acid application,responses to the lagging stimulus were suppressed.Application of gamma-aminobutyric acid affected the normalized response to lagging sounds,independently of whether they or the paired sounds were contralateral or ipsilateral to the recording site.These observations suggest that local inhibition by gamma-aminobutyric acid in the rat inferior colliculus shapes the neural responses to lagging sounds,and modulates the precedence effect. | Yanjun Wang Ningyu Wang Dan Wang Jun Jia Jinfeng Liu Yan Xie Xiaohui Wen Xiaoting Li | 2014 | Neural Regeneration Research2014，9，4： | 4 |
| 8 | Fretting wear behaviour of machined layer of nickel-based superalloy produced by creep-feed profile grinding显示文摘Fretting wear has an adverse impact on the fatigue life of turbine blade roots.The current work is to comparatively investigate the fretting wear behaviour of the nickel-based superalloy surfaces produced by polishing and creep-feed profile grinding,respectively,in terms of surface/subsurface fretting damage,the friction coefficient,wear volume and wear rate.Experimental results show that the granulated tribolayer aggravates the workpiece wear,while the flat compacted tribolayer enhances the wear resistance ability of workpiece,irrespective of whether the workpiece is processed by polishing or grinding.However,the wear behaviors of tribolayers are different.For the polished surface,when the normal load exceeds 100 N,the main defects are crack,rupture,delamination and peeling of workpiece materials;the wear mechanism changes from severe oxidative wear to fatigue wear and abrasive wear when the loads increase from 50 to 180 N.As for the ground surface,the main wear mechanism is abrasive wear.Particularly,the ground surface possesses better wear-resistant ability than the polished surface because the former has the lower values in coefficient friction(0.23),wear volume(0.06×10^(6)μm^(3))and wear rate(0.25×10^(-16)Pa^(-1)).Finally,an illustration is given to characterize the evolution of wear debris on such nickel-based superalloy on the ground surface. | Weijie KUANG Qing MIAO Wenfeng DING Yanjun ZHAO Biao ZHAO Xuebing WEN Shaopeng LI | 2022 | Chinese Journal of Aeronautics2022，35，10： | 3 |
| 9 | A glutathione activatable pro-drug-photosensitizer for combined chemotherapy and photodynamic therapy显示文摘During cancer treatment,chemotherapeutic drugs always result in severe side-effects and drug resistance.Therefore,combining cheomtherapy with other therapeutic modalities,such as photodynamic therapy(PDT)and designing an activable platform is promising for precise and efficient anticancer treatment.Herein,we report a“pro-drug-photosensitizer”agent,LMB-S-CPT,bearing a disulfide bond as the glutathione(GSH)-activatable linker.LMB-S-CPT can be selectively activated by GSH to release activated drug,camptothecin(CPT),for chemotherapy and activated photosensitizer,methylene blue(MB),for PDT.LMB-S-CPT exhibits excellent tumor-activatable performance when injected into tumor-bearing mice,as well as specific cancer therapy with negligible toxic side effects.The activatable pro-drug-photosensitizer offers a new strategy for chemo-photodynamic therapy and displays precise,selective and excellent antitumor effect. | Yanjun Yang Yifeng Zhang Ran Wang Xiang Rong Ting Liu Xiang Xia Jiangli Fan Wen Sun Xiaojun Peng | 2022 | Chinese Chemical Letters2022，33，10： | 2 |
| 10 | Effect of heat treatment on microstructures and properties of a novel Al-Zn-Mg-Cu alloy for oil drilling显示文摘In view of the special requirements for strength, heat resistance and corrosion resistance of Al-Zn-Mg-Cu alloy for oil drilling, the Al-6.2 Zn-2.5 Mg-1.6 Cu alloy was prepared by increasing Cu content on basis of Russian Series 1953 alloy. The effect of heat treatment on the microstructures and properties of the alloy was characterized by optical microscope(OM), scanning electron microscope(SEM) and transmission electron microscope(TEM), and investigated by tensile test at room temperature, thermal exposure test and corrosion test. The results show that the strength after T6 aging treatment exhibit a decrease trend as an increase of the solution temperature from465 ℃ to 480 ℃. After the solution treated by the rate of 470 ℃/1 h, second phases dissolve into the matrix very well and the strength property reaches optimum. The alloy has better comprehensive properties treated by a solution treatment of 470 ℃/1 h and then followed by an aging treatment of 120 ℃/24 h + 170 ℃/1 h + 120 ℃/24 h. Under the aging state, the precipitated phases inside the grains are suitable in size, while on the grain boundary distribute discontinuously and the precipitate-free zone is obvious. Besides, the alloy still maintain high tensile properties. The yield strength, tensile strength and elongation are 650 MPa, 686 MPa,12.0%, respectively. The yield strength retention after heat exposure is 92%. The alloy has good corrosion resistance and the exfoliation corrosion degree. The average corrosion rate in the H2S and CO2 environment is 0.0024 mm/a, which is far less than the required 0.12 mm/a. It is insensitive to H2S and CO2 environments. | Yanjun Fu Xiwu Li Kai Wen Shuhui Huang Yang Dong Mao Rao Fubo Yang Yugang Wang Baiqing Xiong Zhihui Li | 2019 | Progress in Natural Science:Materials International2019，29，2： | 2 |
| 11 | More efficient automatic repair of large-scale programs using weak recompilation显示文摘Automatically repairing a bug can be a time-consuming process especially for large-scale programs owing to the significant amount of time spent recompiling and reinstalling the patched program.To reduce this time overhead and speed up the repair process,in this paper we present a recompilation technique called weak recompilation.In weak recompilation,we assume that a program consists of a set of components,and for each candidate patch only the altered components are recompiled to a shared library.The original program is then dynamically updated by a function indirection mechanism.The advantage of weak recompilation is that redundant recompilation cost can be avoided,and while the reinstallation cost is completely eliminated as the original executable program is not modified at all.For maximum applicability of weak recompilation we created WAutoRepair,a scalable system for fixing bugs with high efficiency in large-scale C programs.The experiments on real bugs in widely used programs show that our repair system significantly outperforms Genprog,a wellknown approach to automatic program repair.For the wireshark program containing over 2 million lines of code,WAutoRepair is over 128 times faster in terms of recompilation cost than Genprog. | QI YuHua MAO XiaoGuang WEN YanJun DAI ZiYing GU Bin | 2012 | Science China(Information Sciences)2012，55，12： | 2 |
| 12 | Associationbe- tweenpl6 INK4apromoter methylation and non-small cell- lungcancer: ameta - analysis 显示文摘 | Jundong Gu Yanjun wen Siwei zhu | 2013 | PLoS One2013，8，60： | 1 |
| 13 | Multi-platform omics analysis reveals molecular signature for COVID-19 pathogenesis,prognosis and drug target discovery显示文摘Disease progression prediction and therapeutic drug target discovery for Coronavirus disease 2019(COVID-19)are particularly important,as there is still no effective strategy for severe COVID-19 patient treatment.Herein,we performed multi-platform omics analysis of serial plasma and urine samples collected from patients during the course of COVID-19.Integrative analyses of these omics data revealed several potential therapeutic targets,such as ANXA1 and CLEC3B.Molecular changes in plasma indicated dysregulation of macrophage and suppression of T cell functions in severe patients compared to those in non-severe patients.Further,we chose 25 important molecular signatures as potential biomarkers for the prediction of disease severity.The prediction power was validated using corresponding urine samples and plasma samples from new COVID-19 patient cohort,with AUC reached to 0.904 and 0.988,respectively.In conclusion,our omics data proposed not only potential therapeutic targets,but also biomarkers for understanding the pathogenesis of severe COVID-19. | Yuming Li Guixue Hou Haibo Zhou Yanqun Wang Hein Min Tun Airu Zhu Jingxian Zhao Fei Xiao Shanwen Lin Dongdong Liu Dunrong Zhou Lang Mai Lu Zhang Zhaoyong Zhang Lijun Kuang Jiao Guan Qiushi Chen Liyan Wen Yanjun Zhang Jianfen Zhuo Fang Li Zhen Zhuang Zhao Chen Ling Luo Donglan Liu Chunke Chen Mian Gan Nanshan Zhong Jincun Zhao Yan Ren Yonghao Xu | 2021 | Signal Transduction and Targeted Therapy2021，6，5： | 1 |
| 14 | Negative regulation of gamma-aminobutyric acid type A receptor on free calcium ion levels following facial nerve injury显示文摘Previous studies have demonstrated that muscarinic,and nicotinic receptors increase free Ca2+ levels in the facial nerve nucleus via various channels following facial nerve injury.However,intracellular Ca2+ overload can trigger either necrotic or apoptotic cell death.Gamma-aminobutyric acid(GABA),an important inhibitory neurotransmitter in the central nervous system,exists in the facial nerve nucleus.It is assumed that GABA negatively regulates free Ca2+ levels in the facial nerve nucleus.The present study investigated GABA type A(GABAA) receptor expression in the facial nerve nucleus in a rat model of facial nerve injury using immunohistochemistry and laser confocal microscopy,as well as the regulatory effects of GABAA receptor on nicotinic receptor response following facial nerve injury.Subunits α1,α3,α5,β1,β2,δ,and γ3 of GABAA receptors were expressed in the facial nerve nucleus following facial nerve injury.In addition,GABAA receptor expression significantly inhibited the increase in nicotinic receptor-mediated free Ca2+ levels in the facial nerve nucleus following facial nerve injury in a concentration-dependent fashion.These results suggest that GABAA receptors exhibit negative effects on nicotinic receptor responses following facial nerve injury. | Fugao Zhu Dawei Sun Yanqing Wang Rui Zhou Junfeng Wen Xiuming Wan Yanjun Wang Banghua Liu | 2010 | Neural Regeneration Research2010，5，22： | 1 |
| 15 | Data Race Detection for Interrupt-Driven Programs显示文摘 | Wu Xueguang Wen Yanjun Chen Liqian | 2013 | IEEE2013，33，： | 1 |
| 16 | Polychromatic full-polarization control in mid-infrared light显示文摘Objects with different shapes,materials and temperatures can emit distinct polarizations and spectral information in mid-infrared band,which provides a unique signature in the transparent window for object identification.However,the crosstalk among various polarization and wavelength channels prevents from accurate mid-infrared detections at high signal-to-noise ratio.Here,we report full-polarization metasurfaces to break the inherent eigen-polarization constraint over the wavelengths in mid-infrared.This recipe enables to select arbitrary orthogonal polarization basis at individual wavelength independently,therefore alleviating the crosstalk and efficiency degradation.A six-channel all-silicon metasurface is specifically presented to project focused mid-infrared light to distinct positions at three wavelengths,each with a pair of arbitrarily chosen orthogonal polarizations.An isolation ratio of 117 between neighboring polarization channels is experimentally recorded,exhibiting detection sensitivity one order of magnitude higher than existing infrared detectors.Remarkably,the high aspect ratio~30 of our meta-structures manufactured by deep silicon etching technology at temperature−150℃ guarantees the large and precise phase dispersion control over a broadband from 3 to 4.5μm.We believe our results would benefit the noise-immune mid-infrared detections in remote sensing and space-to-ground communications. | Jin Chen Feilong Yu Xingsi Liu Yanjun Bao Rongsheng Chen Zengyue Zhao Jiuxu Wang Xiuxia Wang Wen Liu Yuzhi Shi Cheng-Wei Qiu Xiaoshuang Chen Wei Lu Guanhai Li | 2023 | Light(Science & Applications)2023，12，5： | 1 |
| 17 | When do salary and job level predict career satisfaction and turnover intention among Chinese managers? The role of perceived organizational career management and career anchor显示文摘 | Yanjun Guan Yueran Wen Sylvia Xiaohua Chen Haiyang Liu Wei Si Yuhan Liu Yanan Wang Ruchunyi Fu Yuyan Zhang Zhilin Dong | 2014 | European Journal of Work and Organizational Psychology2014，，4： | 1 |
| 18 | HEV-capsid protein interacts with cytochrome P4502C8 and retinol-binding protein 4显示文摘 | Shen Quan Zhang Wen Kang Yanjun | 2011 | Hepat Mon2011，11，11： | 1 |
| 19 | Muscarinic receptor-mediated calcium changes in a rat model of facial nerve nucleus injury显示文摘The muscarinic receptor modulates intracellular free calcium ion levels in the facial nerve nucleus via different channels.In the present study,muscarinic receptor-mediated free calcium ions levels were detected by confocal laser microscopy in the facial nerve nucleus following facial nerve injury in rats.There was no significant difference in muscarinic receptor expression at the affected facial nerve nucleus compared with expression prior to injury,but muscarinic receptor-mediated free calcium ion levels increased in the affected side following facial nerve injury (P < 0.01).At day 30 after facial nerve injury,50 μmol/L muscarinic-mediated free calcium ion levels were significantly inhibited at the affected facial nerve nucleus in calcium-free artificial cerebrospinal fluid,and the change range was 82% of artificial cerebrospinal fluid (P < 0.05).These results suggest that increased free calcium ion concentrations are achieved by intracellular calcium ion release,and that the transmembrane flow of calcium ions is also involved in this process. | Dawei Sun Huamin Liu Fugao Zhu Yanqing Wang Junfeng Wen Rui Zhou Yanjun Wang Banghua Liu | 2010 | Neural Regeneration Research2010，5，18： | 1 |
| 20 | A multiple-proxy stalagmite record reveals historical deforestation in central Shandong, northern China显示文摘Evaluating anthropogenic impacts on regional vegetation changes during historical time is not only important for a better understanding of the Anthropocene but also valuable in improving the vegetation-climate models.In this study,we analyzed stable isotopes(δ^18O,δ^13C)and trace elements(Mg/Ca,Sr/Ca)of a stalagmite from Huangchao Cave in central Shandong,northern China.230Th and AMS14C dating results indicate the stalagmite deposited during 174BC and AD1810,with a hiatus between AD638 and 1102.Broad similarities of theδ^18O and trace elements in the stalagmite suggest they are reliable precipitation indexes.Theδ^13C of the stalagmite,a proxy of vegetation change,was generally consistent with local precipitation and temperature variations on a centennial-scale before the 15th century.It typically varied from–9.6‰to–6.3‰,indicating climate controlled C3 type vegetation during this period.However,a persistent and marked increasing trend in theδ^13C record was observed since the 15th century,resulting inδ^13C values from–7.7‰to–1.6‰in the next four centuries.This unprecedentedδ^13C change caused by vegetation deterioration cannot be explained by climate change but is fairly consistent with the dramatically increasing population and farmland in Shandong.We suggest that the increasing deforestation and reclamation in central Shandong began to affect vegetation in the mountain region of central Shandong since the 15th century and severely destroyed or even cleared the forest during the 16th–18th century. | Liangcheng TAN Wen LIU Tianli WANG Peng CHENG Jingjie ZANG Xiqian WANG Le MA Dong LI Jianghu LAN R.Lawrence EDWARDS Hai CHENG Hai XU Li AI Yongli GAO Yanjun CAI | 2020 | Science China Earth Sciences2020，63，10： | 1 |
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