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| 1 | Antisense MMP-9 RNA inhibits malignant glioma cell growth in vitro and in vivo显示文摘The matrix-degrading metalloproteinases (MMPs), particularly MMP-9, play important roles in the pathogenesis and development of malignant gliomas. In the present study, the oncogenic role of MMP-9 in malignant glioma cells was investigated via antisense RNA blockade in vitro and in vivo. TJ905 malignant glioma cells were transfected with pcDNA3.0 vector expressing antisense MMP-9 RNA (pcDNA-AS-MMP9), which significantly decreased MMP-9 expression, and cell proliferation was assessed. For in vivo studies, U251 cells, a human malignant glioma cell line, were implanted subcutaneously into 4-to 6-week-old BALB/c nude mice. The mice bearing well-established U251 gliomas were treated with intratumoral pcDNA-AS-MMP9-Lipofectamine complex (AS-MMP-9-treated group), subcutaneous injection of endostatin (endostatin-treated group), or both (combined therapy group). Mice treated with pcDNA (empty vector)-Lipofectamine served as the control group. Four or eight weeks later, the volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity were assayed. We demonstrate that pcDNA-AS-MMP9 significantly decreased MMP-9 expression and inhibited glioma cell proliferation. Volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity in the antisense-MMP-9-treated and therapeutic alliance groups were significantly lower than those in the control group. The results suggest that MMP-9 not only promotes malignant glioma cell invasiveness, but also affects tumor cell proliferation. Blocking the expression of MMP-9 with antisense RNA substantially suppresses the malignant phenotype of glioma cells, and thus can be used as an effective therapeutic strategy for malignant gliomas. | Cuiyun Sun Qian Wang Hongxu Zhou Shizhu Yu Alain R. Simard Chunsheng Kang Yanyan Li Yanling Kong Tongling An Yanjun Wen Fudong Shi Junwei Hao | 2013 | Neuroscience Bulletin2013，29，1： | 14 |
| 2 | Twa1/Gid8 is a β-catenin nuclear retention factor in Wnt signaling and colorectal tumorigenesis显示文摘 | Yi Lu Shanshan Xie Wen Zhang Cheng Zhang Cheng Gao Qiang Sun Yuqi Cai Zhangqi Xu Min Xiao Yanjun Xu Xiao Huang Ximei Wu Wei Liu Fudi Wang Yibin Kang Tianhua Zhou | 2017 | Cell Research2017，27，12： | 9 |
| 3 | Atomistic Surface Passivation of CH_(3)NH_(3)PbI_(3) Perovskite Single Crystals for Highly Sensitive Coplanar-Structure X-Ray Detectors显示文摘Organic-inorganic halide perovskites(OIHPs)are recognized as the promising next-generation X-ray detection materials.However,the device performance is largely limited by the ion migration issue of OIHPs.Here,we reported a simple atomistic surface passivation strategy with methylammonium iodide(MAI)to remarkably increase the ion migration activation energy of CH_(3)NH_(3)PbI_(3)single crystals.The amount of MAI deposited on the crystal surface is finely regulated by a self-assemble process to effectively suppress the metallic lead defects,while not introducing extra mobile ions,which results in significantly improved dark current stability of the coplanar-structure devices under a large electric field of 100Vmm^(-1).The X-ray detectors hence exhibit a record-high sensitivity above 700,000μC Gy^(-1)_(air) cm^(-2)under continuum X-ray irradiation with energy up to 50 keV,which enables an ultralow X-ray detection limit down to 1.5 nGy_(air)s^(-1).Our findings will allow for the dramatically reduced X-ray exposure of human bodies in medical imaging applications. | Yilong Song Liqi Li Weihui Bi Mingwei Hao Yifei Kang Anran Wang Zisheng Wang Hanming Li Xiaohui Li Yanjun Fang Deren Yang Qingfeng Dong | 2020 | Research2020，，1： | 5 |
| 4 | Premium Connection Downhole Tubing Corrosion显示文摘 | Shuanlu Lu Jianmin Xiang Yanjun Kang Zeliang Chang Xunchang Dong Tongguang Zhai | 2008 | Materials Performance2008，，5： | 2 |
| 5 | Preparation and Characterization of Zeolite Beta with Low SiO_2/Al_2O_3 Ratio显示文摘Zeolite beta with a low SiO2/Al2O3 ratio was synthesized by a novel two-step process.The synthesized sample was characterized with XRD,SEM,FTIR,and N2 adsorption-desorption and solid-state MAS NMR.The results showed that aluminium species were inserted into the framework of zeolite beta.The BET surface area,volume,and particle size of the zeolite beta sample decreased with decreasing SiO2/Al2O3 ratio.Our process was proved an efficient route to synthesize zeolite beta with a low SiO2/Al2O3 ratio. | Kang Shanjiao Gong Yanjun Dou Tao Zhang Ying Zheng Yanying | 2007 | Petroleum Science2007，4，1： | 1 |
| 6 | Deep desulfurization of gasoline using ion - exchange zeolltes : Cu ( I ) - and Ag ( I ) - beta 显示文摘 | Gong Yanjun Dou Tao Kang Shanjiao | 2009 | Fuel Processing Technology2009，90，1： | 1 |
| 7 | Deep desulfurization of gasoline using ion-exchange zeolites:Cu(Ⅰ)-and Ag(Ⅰ)-beta显示文摘 | Gong Yanjun Dou Tao Kang Shanjiao | 2009 | Fuel Processing Technology2009，90，1： | 1 |
| 8 | HEV-capsid protein interacts with cytochrome P4502C8 and retinol-binding protein 4显示文摘 | Shen Quan Zhang Wen Kang Yanjun | 2011 | Hepat Mon2011，11，11： | 1 |
| 9 | Premium Connection Downhole Tubing Corrosion显示文摘 | Shuanlu Lu Jianmin Xiang Yanjun Kang Zeliang Chang Xunchang Dong Tongguang Zhai | 2008 | Materials Performance2008，，5： | 1 |
| 10 | Enhanced optical performance of multifocal metalens with conic shapes显示文摘A multifocal metalens,which focuses incident light at multiple foci,has many applications in imaging systems and optical communications.However,the traditional design strategy of a multifocal metalens combines several lenses that have different focal points into a planar integrated unit,resulting in low imaging quality because of the high background noise.Here we show that the defects of the traditional method can be overcome by designing a metalens with conic shapes(the ellipse and the hyperbola);this approach could improve the imaging performance and substantially decrease the background noise of multifocal metalenses.These benefits arise from the intrinsic properties of the two conic curves,which can focus incident light constructively at all of the foci of the metalens.We further demonstrate that the proposed conicshaped metalens can function well within a broadband operation wavelength that ranges from 600 to 900 nm with the dual polarity actively controlled by the incident circular polarized light.The great agreement between the experimental and simulation results demonstrates that our proposed metalens has significant potential for use in future integrated nanophotonic devices. | Yanjun Bao Qiao Jiang Yimin Kang Xing Zhu Zheyu Fang | 2017 | Light(Science & Applications)2017，6，1： | 1 |
| 11 | Konjac glucomannan and xanthan gum as compression coat for colonic drug delivery:experimental and theoretical evaluations显示文摘Compression coated tablets for oral colon specific delivery systems were developed with a mixture polysaccharide of konjac glucomannan(KGM)and xanthan gum(XG)as the compression coat.Diffusion of cimetidine from compression coated tablets was investigated by release experiment in Vitro.0.22U/mLβ-mannanase was applied in the mimic colon solution.The structure of the mixture polysaccharide was studied by an atomic force microscope(AFM).The experimental results indicate that a KGM70 tablet with a 0.4 g coat is of good design,due to a less than 5%drug loss in the mimic upper gastrointestinal solution by the synergistic interaction between XG and KGM,and due to about 50%cumulative release in the mimic colon solution by degradation after 24 hours.The release mechanism and model are discussed based on different periods of drug release including the delay of the drug,the constant release without an enzyme and the delay of degradation.Under hydrolysis byβ-mannanase,drug release from the tablet with KGM coat shows an exponential increase,while that from the dosage with the mixture polysaccharide coat is an approximately zero-order process in which the constant release rate relates to the release velocity of a non-degraded system,the content of KGM within the coat and the average molecular weight ratio of KGM to XG.It was found that XG was the framework of the polysaccharide mixtures by AFM,which is similar to the analysis results from experiments on drug release. | Kang WANG Jiangyang FAN Yanjun LIU Zhimin HE | 2010 | Frontiers of Chemical Science and Engineering2010，4，1： | 0 |
| 12 | A secreted salivary effector from Riptortus pedestris impairs soybean defense through modulating phytohormone signaling pathways显示文摘Riptortus pedestris(Fabricius),one of the major piercing-sucking insects in soybeans,causes delayed plant senescence and abnormal pods,known as staygreen syndrome.Recent research has shown that direct feeding of this insect is the major cause of soybean staygreen syndrome.However,it remains unclear whether R.pedestris salivary proteins play vital roles in insect infestation.Here,we found that 4 secretory salivary proteins can induce cell death in Nicotiana benthamiana by transient heterologous expression.The cell death induced by Rp2155 relies on the nucleotide-binding leucine-rich repeat helper,HSP90.Tissue-specificity assays indicated that Rp2155 is specifically expressed in the salivary gland of R.pedestris and is significantly induced during insect feeding.The expression of salicylic acid(SA)-,jasmonic acid(JA)-related genes was increased in soybean when fed by Rp2155-silenced R.pedestris.More importantly,soybean staygreen symptoms caused by R.pedestris were significantly alleviated when Rp2155 was silenced.Together,these results suggest that the salivary effector Rp2155 is involved in promoting insect infestation by suppressing the JA and SA pathways,and it can be considered as a potential RNA interference target for insect control. | Yue Huang Biao Hu Zhongyan Wei Shiqi Shan Chunyun Guo Hehong Zhang Yanjun Li Jianping Chen Xue Kang Haijian Huang Zongtao Sun | 2023 | Insect Science2023，30，6： | 0 |
| 13 | Enlarged fins of Tibetan catfish provide new evidence of adaptation to high plateau显示文摘The uplift of the Tibetan Plateau significantly altered the geomorphology and climate of the Euroasia by creating large mountains and rivers.Fishes are more likely to be affected relative to other organisms,as they are largely restricted to river systems.Faced with the rapidly flowing water in the Tibetan Plateau,a group of catfish has evolved greatly enlarged pectoral fins with more numbers of fin-rays to form an adhesive apparatus.However,the genetic basis of these adaptations in Tibetan catfishes remains elusive.In this study,we performed comparative genomic analyses based on the chromosome-level genome of Glyptosternum maculatum in family Sisoridae and detected some proteins with conspicuously high evolutionary rates in particular in genes involved in skeleton development,energy metabolism,and hypoxia response.We found that the hoxd12a gene evolved faster and a loss-of-function assay of hoxd12a supports a potential role for this gene in shaping the enlarged fins of these Tibetan catfishes.Other genes with amino acid replacements and signatures of positive selection included proteins involved in low temperature(TRMU)and hypoxia(VHL)responses.Functional assays reveal that the G.maculatum TRMU allele generates more mitochondrial ATP than the ancestral allele found in low-altitude fishes.Functional assays of VHL alleles suggest that the G.maculatum allele has lower transactivation activity than the low-altitude forms.These findings provide a window into the genomic underpinnings of physiological adaptations that permit G.maculatum to survive in the harsh environment of the Tibetan Himalayas that mirror those that are convergently found in other vertebrates such as humans. | Liandong Yang Ning Sun Honghui Zeng Ying Wang Wenjun Chen Zufa Ding Yang Liu Jing Wang Minghui Meng Yanjun Shen Jingliang Kang Xiuhui Ma Wenqi Lv Juan Chen Axel Meyer Baocheng Guo Shunping He | 2023 | Science China(Life Sciences)2023，66，7： | 0 |
| 14 | Incineration experiment of medical waste of novel coronavirus pneumonia(COVID-19)in a mobile animal carcass incinerator显示文摘Incineration experiment of medical waste was carried out in a mobile animal carcass incinerator.Simulated medical waste(69%cotton,1.5%wood product,4.5%mask and 25%moisture)was used as raw material.The temperature trend of first and second combustion chamber,the operating conditions and the emission characteristics of gaseous pollutants were studied.The results indicated that the temperature of first combustion chamber can be maintained at 550-650℃ without external heating,while in the final stage a burner was used to realize the burnout of material.The temperature of the second combustion chamber was always lower than that of the first combustion after the burner stopped working.The concentration of CO emission in flue gas was high due to the low disposal efficiency of the mobile incinerator,while NOX and SO2 emission concentrations were far below the standard limit value(GB 18484-2001). | Shaorui Zhang Yanjun Zhang Fengjun Wang Da Kang Jingjie Wang Minghao Wang Chunwei Zhang Yongtang Wang Hong Liu Baodong Zhou Yong Wu | 2021 | Waste Disposal and Sustainable Energy2021，3，2： | 0 |
| 15 | Feedback is all you need:from ChatGPT to autonomous driving显示文摘The birth of chat generative pre-trained transformer(ChatGPT).In November 2022,Microsoft and OpenAI jointly released an artificial intelligence chatbot program called ChatGPT[1].In just two months,the number of global users surpassed 100 million,making it the fastestgrowing consumer application in history. | Hong CHEN Kang YUAN Yanjun HUANG Lulu GUO Yulei WANG Jie CHEN | 2023 | Science China(Information Sciences)2023，66，6： | 0 |
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