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| 1 | Helicobacter pylori vac A genotype is a predominant determinant of immune response to Helicobacter pylori CagA显示文摘AIM To evaluate the frequency of Helicobacter pylori(H. pylori) Cag A antibodies in H. pylori infected subjects and to identify potential histopathological and bacterial factors related to H. pylori Cag A-immune response.METHODS Systematic data to H. pylori isolates, blood samples, gastric biopsies for histological and molecular analyses were available from 99 prospectively recruited subjects. Serological profile(anti-H. pylori, anti-Cag A) was correlated with H. pylori isolates(cag A, EPIYA, vac A s/m genotype), histology(Sydney classification) and mucosal interleukin-8(IL-8) m RNA and protein expression. Selected H. pylori strains were assessed for H. pylori Cag A protein expression and IL-8 induction in co-cultivation model with AGS cells.RESULTS Thirty point three percent of microbiologically confirmed H. pylori infected patients were seropositive for Cag A. Majority of H. pylori isolates were cag A gene positive(93.9%) with following vac A polymorphisms: 42.4% vac A s1m1, 23.2% s1m2 and 34.3% s2m2. Anti-Cag AIg G seropositivity was strongly associated with atrophic gastritis, increased mucosal inflammation according to the Sydney score, IL-8 and cag A m RNA expression. V a c A s a n d m p o l y m o r p h i s m s w e r e t h e m a j o r determinants for positive(vac A s1m1) or negative(vac A s2m2) anti-Cag A serological immune response, which also correlated with the in vitro inflammatory potential in AGS cells. In vitro co-cultivation of representative H. pylori strains with AGS cells confirmed functional Cag A translocation, which showed only partial correlation with Cag A seropositivity in patients, supporting vac A as major co-determinant of the immune response.CONCLUSION Serological immune response to H. pylori cag A + strain in H. pylori infected patients is strongly associated with vac A polymorphism, suggesting the crucial role of bacterial factors in immune and clinical phenotype of the infection. | Alexander Link Cosima Langner Wiebke Schirrmeister Wiebke Habendorf Jochen Weigt Marino Venerito Ina Tammer Dirk Schlüter Philipp Schlaermann Thomas F Meyer Thomas Wex Peter Malfertheiner | 2017 | World Journal of Gastroenterology2017，23，26： | 12 |
| 2 | Different antibiotic susceptibility between antrum and corpus of the stomach,a possible reason for treatment failure of Helicobacter pylori infection显示文摘AIM:To assess whether antibiotic resistance varies between the antrum and corpus of the stomach of patients that are either Helicobacter pylori(H.pylori)therapy-naive or pre-treated.METHODS:H.pylori strains were isolated from antrum and corpus biopsies from 66 patients that received a diagnostic gastroduodenoscopy for variant clinical indications.Antimicrobial susceptibility to amoxicillin,clarithromycin,tetracycline,metronidazole,levofloxacin and rifabutin was tested with the E-test method on IsoSensitest agar with 10 vol%defibrinated horse blood.In patients with a different antibiotic susceptibility pattern between the isolates from the antrum and corpus,DNA fingerprinting via random amplified polymorphic DNA analysis was performed to detect differences among DNA patterns of H.pylori isolates.RESULTS:Primary,secondary and tertiary resistance to clarithromycin was 6.9%,53.8%and 83.3%,retrospectively.Metronidazole and levofloxacin resistance also increased according to the number of previous treatments(17.2%,69.2%,83.3%;13.8%,23.1%,33.3%).Tertiary resistance to rifabutin was detected in12.5%of patients.In none of the 66 patients a resistance against amoxicillin or tetracycline was detectable.Discordant antibiotic susceptibility between antrum and corpus isolates for different antibiotics was seen in 15.2%(10/66)of the patients.Two out of those ten patients were naive to any H.pylori antibiotic treatment.The remaining eight patients previously received at least one eradication therapy.DNA fingerprinting analysis revealed no substantial differences among DNA patterns between antrum and corpus isolates in the majority of patients suggesting an infection with a single H.pylori strain.CONCLUSION:Different antibiotic susceptibility between antrum and corpus biopsies is a common phenomenon and a possible explanation for treatment failure.Resistant H.pylori strains may be missed if just one biopsy from one anatomic site of the stomach is taken for H.pylori susceptibility testing. | Michael Selgrad Ina Tammer Cosima Langner Jan Bornschein Julia Mei?le Arne Kandulski Mariya Varbanova Thomas Wex Dirk Schlüter Peter Malfertheiner | 2014 | World Journal of Gastroenterology2014，20，43： | 10 |
| 3 | Expression of cytokeratins in Helicobacter pylori-associated chronic gastritis of adult patients infected with cagA+strains:An immunohistochemical study显示文摘瞄准:为了与长期的胃炎在成年病人的胃的上皮调查不同 cytokeratins (CK ) 的表示,与 Helicobacter pylori (H pylori ) 感染了 cagA+ 紧张。方法:CK 7 的表示, 8, 18, 19 和 20 在 84 个病人的窦的胃的活体检视组织化学地是学习免疫。所有 CK 是在 cagA+H pylori 胃炎(57 个案例) 染色的免疫, non-H pylori 胃炎(17 个案例) 和正常胃粘膜(10 个案例) 。结果:在 cagA+ H pylori 胃炎, CK8 从表面上皮可比较地被表示到正常的窦粘膜到深腺。CK18 和 CK 19 的分发是未改变的,即表示的 transmucosal,而是紧张在与正常相比的小凹的区域是不同的胃粘膜。Cytokeratin 18 免疫反应在与 H pylori 否定的胃炎和控制相比的 H pylori 积极的胃炎的小凹的上皮是显著地更高的。相反,在 CK19 免疫反应的减少发生在 H pylori 积极的胃炎的小凹的上皮。在没有 H pylori 感染的正常、煽动的窦粘膜, CK20 在表面上皮和上面的小凹的区域强烈 / 中等并且同类地被表示,但是在 H, pylori 导致了胃炎在小凹的区域的表示的重要减少被注意。通常,在正常窦的粘膜和 H pylori 否定的胃炎,, CK7 的表示没被观察在关于半 cagA+ , H 感染 pylori 的病人,节制颈的焦点的 CK7 免疫反应,卷的腺区域被登记,特别在区域与更严重煽动性渗入。结论:在 CK 7 的表示的改变, 18, 19 和 20 在感染 cagA+ 紧张的成年病人和 CK8 的正常表示发生在 H 联系 pylori 的长期的胃炎的窦粘膜。在不同 cytokeratins 表示力量的改变贡献在 H 感染 pylori 的胃粘膜观察的上皮的紧密的连接变弱。 | Vera Todorovic Neda Drndarevic Olivera Mitrovic Institutefor MedicalResearch Aleksandra Sokic-Milutinovic Tomica Milosavljevic Marjan Micev Ivan Nikolic Thomas Wex Peter Malfertheiner | 2006 | World Journal of Gastroenterology2006，12，12： | 2 |
| 4 | Differential Expression of Human Beta Defensin 2 and 3 in Gastric Mucosa of H elicobacter pylori ‐Infected Individuals显示文摘 | Bianca Bauer Thomas Wex Doerthe Kuester Thomas Meyer Peter Malfertheiner | 2012 | Helicobacter2012，，1： | 2 |
| 5 | Polymorphisms of micro RNA target genes IL12B, INSR, CCND1 and IL10 in gastric cancer显示文摘AIM To evaluate associations between mi RNA target genes IL12B,INSR,CCND1 and IL10 polymorphisms and gastric cancer(GC)in European population.METHODS Gene polymorphisms were analyzed in 508 controls and474 GC patients from 3 tertiary centers in Germany,Lithuania and Latvia.Controls were patients from the out-patient departments,who were referred for upper endoscopy because of dyspeptic symptoms and had no history of previous malignancy.Gastric cancer(GC)patients had histopathological verification of gastric adenocarcinoma.Genomic DNA was extracted using salting out method from peripheral blood mononuclear cells.IL12B T>G(rs1368439),INSR T>C(rs1051690),CCND1 A>C(rs7177)and IL10 T>C(rs3024498)SNPs were genotyped by the real-time polymerase chain reaction.Associations between gene polymorphism and GC were evaluated using multiple logistic regression analysis with adjustment for sex,age and country of birth.RESULTS We observed similar distribution of genotypes and allelic frequencies of all polymorphisms between GC patients and controls except of INSR rs1051690.The frequency of the T allele of INSR gene was significantly higher in GC patients than in controls(23.26%and 19.19%respectively,P=0.028).CT genotype was also more prevalent in patients compared to control group(38.48%and 30.12%respectively,P<0.021).Logistic regression analysis revealed that only one polymorphism(rs1051690 in INSR gene)was associated with increased risk of GC.Carriers of CT genotype had higher odds of GC when compared to CC genotype(OR=1.45,95%PI:1.08-1.95,P=0.01).Similar association was observed in a dominant model for INSR gene,where comparison of TT+CT vs CC genotypes showed an increased risk of GC(OR=1.44,95%PI:1.08-1.90,P=0.01).Other analyzed SNPs were not associated with the presence of GC.CONCLUSION INSR rs1051690 SNP is associated with increased risk of GC,while polymorphisms in IL12B,CCND1 and IL10genes are not linked with the presence of GC. | Vytenis Petkevicius Violeta Salteniene Simonas Juzenas Thomas Wex Alexander Link Marcis Leja Ruta Steponaitiene Jurgita Skieceviciene Limas Kupcinskas Laimas Jonaitis Gediminas Kiudelis Peter Malfertheiner Juozas Kupcinskas | 2017 | World Journal of Gastroenterology2017，23，19： | 2 |
| 6 | Atosiban versus betamimeties in the treatment of preterm labour in Italy: Clinical and economic importanee of side-effeets显示文摘 | Wex J Abou-Setta AM Clerici G | 2011 | Eur J Obstet Gyneeol Reprodue Bio2011，157，2： | 1 |
| 7 | Serological assessment of gastric mucosal atrophy in gastric cancer显示文摘 | Jan Bornschein Michael Selgrad Thomas Wex et : | 2012 | BMC Gastroenterology2012，12，10： | 1 |
| 8 | Correlation of serum pepsinogens and gastrin-17 with atrophic gastritis in gastroesophageal reflux patients:a matched-pairs study显示文摘 | PEITZ U WEX T VIETH M | | 0，，： | 1 |
| 9 | Lack of associa-tion between gene polymorphisms of Angiotensin converting enzyme,Nod-like receptor 1,Toll-like receptor 4,FAS/FASL and the presence of Helicobacter pylori-induced pre-malignant gastric lesions and gastric cancer in Caucasians 显示文摘 | Kupcinskas J Wex T Bornschein J | 2011 | BMC Med Genet2011，12，： | 1 |
| 10 | Interleukin-1B and interleukin-1 receptor antagonist gene polymorphisms are not associated with premalignant gastric conditions: a combined haplotype analysis显示文摘 | Limas Kupcinskas Thomas Wex Juozas Kupcinskas Marcis Leja Audrius Ivanauskas Laimas Virgilijus Jonaitis Dainius Janciauskas Gediminas Kiudelis Konrads Funka Agnese Sudraba Han-Mo Chiu Jaw-Town Lin Peter Malfertheiner | 2010 | European Journal of Gastroenterology & Hepatology2010，，10： | 1 |
| 11 | Helicobacter pylori-mediated gastritis induces local downregulation of secretory leukocyte protease inhibitor in the antrum显示文摘 | Wex T Treiber G Nilius M | 2004 | Infect Immun2004，72，4： | 1 |
| 12 | Modeofactionofnintedanibinthetreatmentofidiopathicpulmonaryfibrosis显示文摘 | WollinL WexE PautschA etal | 2015 | EurRespirJ2015，45，5： | 1 |
| 13 | Differential expression of human beta defensin 2 and 3 in gastric mucosa of Helicobacter pylori-infected \ndividuals显示文摘 | Bauer B Wex T Kuester 0 | 2013 | Helicobacter2013，18，1： | 1 |
| 14 | Cloning, characterization, and expression of the human TINag-RP gene encoding a novel putative extracellular matrix protein? 显示文摘 | Brtinm NC Wex T Wex H | 2000 | Biochem Biophy Re Commtmications2000，271，2： | 1 |
| 15 | TINag-RP, a novel catalytically inactive cathepsinB-related protein with EGF domains, is predominantlyexpressed in vascular smooth muscle cells 显示文摘 | Wex TI Lipyansky A Bmme NC | 2001 | Biochemistry2001，40，5： | 1 |
| 16 | Antibiotic susceptibility of Helicobacter pylori in central Germany and its relationship with the number of eradication therapies显示文摘 | Michael Selgrad Julia Meile Jan Bornschein Arne Kandulski Cosima Langner Mariya Varbanova Thomas Wex Ina Tammer Dirk Schlüter Peter Malfertheiner | 2013 | European Journal of Gastroenterology & Hepatology2013，，11： | 1 |
| 17 | Functional involvement of cathepsin W in the cytotoxic activity of NK-92 cells显示文摘 | Wex T Wex H Hartig R | 2003 | FEBS Lett2003，552，23： | 1 |
| 18 | , Interleukin-lB and interleukin-1 receptor antagonist gene polymorphisms are not associ- ated with premalignant gastric conditions: a combined haplotype analysis显示文摘 | Kupcinskas L Wex T Kupcinskas J | 2010 | EurJ Gastroenterol Hepatol2010，22，10： | 1 |
| 19 | Proteinase- activated receptor2 in the pathogenesis of gastroesophageal reflux disease显示文摘 | Kandulski A Wex T M6nkemtiller K | 2010 | Am J Gastroenterol2010，105，9： | 1 |
| 20 | Expression of components of the IGF signalling system in childhood acute lympho blastic leukaemia显示文摘 | Vorwerk P Wex H Hohmann B | 2002 | Mol Pathol2002，55，1： | 1 |
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