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| 1 | A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria显示文摘There has been a considerable development in microfluidic based immunodiagnostics over the past few years which has greatly favored the growth of novel point-of-care-testing(POCT).However,the realization of an inexpensive,low-power POCT needs cheap and disposable microfluidic devices that can perform autonomously with minimum user intervention.This work,for the first time,reports the development of a new microchannel capillary flow assay(MCFA)platform that can perform chemiluminescence based ELISA with lyophilized chemiluminescent reagents.This new MCFA platform exploits the ultra-high sensitivity of chemiluminescent detection while eliminating the shortcomings associated with liquid reagent handling,control of assay sequence and user intervention.The functionally designed microchannels along with adequate hydrophilicity produce a sequential flow of assay reagents and autonomously performs the ultra-high sensitive chemiluminescence based ELISA for the detection of malaria biomarker such as PfHRP2.The MCFA platform with no external flow control and simple chemiluminescence detection can easily communicate with smartphone via USB-OTG port using a custom-designed optical detector.The use of the smartphone for display,data transfer,storage and analysis,as well as the source of power allows the development of a smartphone based POCT analyzer for disease diagnostics.This paper reports a limit of detection(LOD)of 8 ng/mL by the smartphone analyzer which is sensitive enough to detect active malarial infection.The MCFA platform developed with the smartphone analyzer can be easily customized for different biomarkers,so a hand-held POCT for various infectious diseases can be envisaged with full networking capability at low cost. | Sthitodhi Ghosh Kashish Aggarwal Vinitha T.U Thinh Nguyen Jungyoup Han Chong H.Ahn | 2020 | Microsystems & Nanoengineering2020，6，1： | 7 |
| 2 | All-epiphyseal versus trans-epiphyseal screw fixation for tillaux fractures:Does it matter?显示文摘BACKGROUND Tillaux fractures occur primarily in adolescents due to the pattern of physeal closure and are classified as Salter-Harris type III physeal fractures.Operative management with screw fixation is recommended for more than 2 mm of displacement or more than 1 mm of translation.However,the efficacy and complications of trans-physeal vs all-physeal screw fixation have not been investigated extensively.AIM To compare the clinical and functional outcomes of trans-physeal(oblique)and all-epiphyseal(parallel)screw fixation in management of Tillaux fractures among pediatric patients.METHODS This was an ethics board approved retrospective review of pediatric patients who presented to our tertiary children’s care facility with Tillaux fractures.We included patients who had surgical fixation of a Tillaux fracture over a 10 year period.Data analysis included demographics,mode of injury,management protocols,and functional outcomes.The patients were divided into group 1(oblique fixation)and group 2(parallel fixation).Baseline patient characteristics and functional outcomes were compared between groups.Statistical tests to evaluate differences included Fisher’s Exact or Chi-squared and independent samples t or Mann Whitney tests for categorical and continuous variables,respectively.RESULTS A total of 42 patients(28 females and 14 males)were included.There were no significant differences in body mass index,sex,age,or time to surgery between the groups[IK2].Sports injuries accounted for 61.9%of the cases,particularly non-contact(57.1%)and skating(28.6%)injuries.Computed Tomography(CT)scan was ordered for 28 patients(66.7%),leading to diagnosis confirmation in 17 patients and change in management plan in 11 patients.[GRC3]Groups 1 and 2 consisted of 17 and 25 patients,respectively.For mid to long-term functional outcomes,there were 14 and 10 patients in groups 1 and 2,respectively.Statistical analysis revealed no significant differences in the functional outcomes,pain scores,or satisfaction between groups.No infections,non-unions,physeal arrest,or post-operative ankle deformities were reported.Two(4.8%)patients had difficulty returning to sports post-surgery due to pain.One was a dancer,and the other patient had pain while running,which led to hardware removal.Both patients had parallel fixation.Hardware removal for groups 1 and 2 were 4(23.5%)and 5(20.0%)patients,respectively.The reasons for removal was pain in 2 patients,and parental preference in the remaining.CONCLUSION This is the largest reported series of pediatric patients with Tillaux fractures comparing functional outcomes of different methods of screw fixation orientation to the physis,which showed no difference regarding functional outcomes. | Brett Heldt Isaiah Roepe Raymond Guo Elsayed Attia Ifeoma Inneh Vinitha Shenava Indranil Kushare | 2022 | World Journal of Orthopedics2022，13，2： | 2 |
| 3 | Prospects for discriminating Zingiberaceae species in India using DNA barcodes显示文摘We evaluated nine plastid(matK, rbcL, rpoC1, rpoB,rpl36-rps8, ndhJ, trnL-F, trnH-psbA, accD) and two nuclear(ITS and ITS2) barcode loci in family Zingiberaceae by analyzing 60 accessions of 20 species belonging to seven genera from India.Bidirectional sequences were recovered for every plastid locus by direct sequencing of polymerase chain reaction(PCR)amplicons in all the accessions tested. However, only 35(58%)and 40 accessions(66%) yielded ITS and ITS2 sequences,respectively, by direct sequencing. In different bioinformatics analyses, matK and rbcL consistently resolved 15 species(75%)into monophyletic groups and five species into two paraphyletic groups. The 173 ITS sequences, including 138 cloned sequences from 23 accessions, discriminated only 12 species(60%), and the remaining species were entered into three paraphyletic groups. Phylogenetic and genealogic analyses of plastid and ITS sequences imply the possible occurrence of natural hybridizations in the evolutionary past in giving rise to species paraphyly and intragenomic ITS heterogeneity in the species tested. The results support using matK and rbcL loci for barcoding Zingiberaceae members and highlight the poor utility of ITS and the highly regarded ITS2 in barcoding this family, and also caution against proposing ITS loci for barcoding taxa based on limited sampling. | Meenakshi Ramaswamy Vinitha Unnikrishnan Suresh Kumar Kizhakkethil Aishwarya Mamiyil Sabu George Thomas | 2014 | Journal of Integrative Plant Biology2014，56，8： | 2 |
| 4 | Third Order Nonlinear Optical Properties and Optical Limiting Behavior of Alkali Metal Complexes of p-Nitrophenol 显示文摘 | Thangaraj M Vinitha G Sabari Girisun T C | 2015 | Opt Laser Technol2015，73，5： | 1 |
| 5 | Non-invasive automated 3D thyroid lesion classification in ultrasound: A class of ThyroScan? systems显示文摘 | U. Rajendra Acharya S. Vinitha Sree M. Muthu Rama Krishnan Filippo Molinari Roberto Garberoglio Jasjit S. Suri | 2011 | Ultrasonics2011，，4： | 1 |
| 6 | Bone grafts in dentistry显示文摘 | Kumar P Vinitha B Fathima G | 2013 | J Pharm Bioallied Sci2013，5，1： | 1 |
| 7 | Antifibrotic Effects of Antioxidant N-Acetylcysteine in a Mouse Model of Human Hypertrophic Cardiomyopathy Mutation显示文摘 | Ali J. Marian Vinitha Senthil Suet N. Chen Raffaella Lombardi | 2006 | Journal of the American College of Cardiology2006，，4： | 1 |
| 8 | Prevention of Cardiac Hypertrophy by Atorvastatin in a Transgenic Rabbit Model of Human Hypertrophic Cardiomyopathy显示文摘 | Vinitha Senthil Suet N. Chen Natalie Tsybouleva Tripti Halder Sherif F. Nagueh James T. Willerson Robert Roberts A J. Marian | 2005 | Circulation Research2005，，3： | 1 |
| 9 | Diagnosis of Hashimoto's thyroiditis in ultrasound using tissue characterization and pixel classification显示文摘 | Acharya U R Vinitha Sree S Mookiah M R et at | 2013 | Proc Inst Mech Eng H2013，227，7： | 1 |
| 10 | Optimum Microemulsions Formulated with Propoxylated Guerbet Alcohol and Propoxylated Tridecyl Alcohol Sodium Sulfates显示文摘 | William H Wade Vinitha Weerasooriya | 1995 | Dispersion Sci Technol1995，316，2： | 1 |
| 11 | A comparative study of radioprotection by Ocimum flavonoids and synthetic aminothiol protectors in the mouse 显示文摘 | Devi P U Bisht K S Vinitha M | 1998 | British Journal of Radiology1998，71，847： | 1 |
| 12 | A comparative study of radioprofection by ocimun flavonoids and synthetic aminothol protectors in the mouse显示文摘 | Devi PU Bisht KS vinitha M | 1998 | Br J Radiol1998，71，： | 1 |
| 13 | Comparison of dimensional accuracy of four different die materials before and after disinfection of the impression:an in vitro study显示文摘 | Nandini Y Vinitha KB Manvi S | 2013 | J Contemp Dent Pract2013，14，4： | 1 |
| 14 | Prevention of cardiac hypertrophy by atorvastatin in a transgenic rabbit model of human hypertrophic cardiomyopathy显示文摘 | Vinitha S Chen SN Natalie T | 2005 | Circ Res2005，97，3： | 1 |
| 15 | Chlorination for power plant biofouling control: Potential impact on entrained phytoplankton 显示文摘 | VINITHA E VEERAMANI P VENUGOLALAN V | 2010 | Environmental Studies2010，67，4： | 1 |
| 16 | Web service recom- mendation systems with reputation evaluation and malicious feed- back estimation prevention显示文摘 | PRIYANKA T ROJA M VINITHA N | 2015 | International journal of research in computer applications and robotics2015，3，3： | 1 |
| 17 | Prevention of cardiac hypertrophy by atorvastatin in a transgenic rab bit model of human hypertrophic cardiomyopathy 显示文摘 | Vinitha S Suet N Chen Natalie T | 2005 | Circ Res2005，97，3： | 1 |
| 18 | UHRF1 Overexpression Drives DNA Hypomethylation and Hepatocellular Carcinoma显示文摘 | Raksha Mudbhary Yujin Hoshida Yelena Chernyavskaya Vinitha Jacob Augusto Villanueva M. Isabel Fiel Xintong Chen Kensuke Kojima Swan Thung Roderick T. Bronson Anja Lachenmayer Kate Revill Clara Alsinet Ravi Sachidanandam Anal Desai Sucharita SenBanerjee Ch | 2014 | Cancer Cell2014，，2： | 1 |
| 19 | Secretive derived from hypoxia preconditioned mesenchymal stem cells promote cartilage regeneration and mitigate joint inflammation via extracellular vesicles显示文摘Secretome derived from mesenchymal stem cells (MSCs) have profound effects on tissue regeneration, which could become the basis of future MSCs therapies. Hypoxia, as the physiologic environment of MSCs, has great potential to enhance MSCs paracrine therapeutic effect. In our study, the paracrine effects of secretome derived from MSCs preconditioned in normoxia and hypoxia was compared through both in vitro functional assays and an in vivo rat osteochondral defect model. Specifically, the paracrine effect of total EVs were compared to that of soluble factors to characterize the predominant active components in the hypoxic secretome. We demonstrated that hypoxia conditioned medium, as well as the corresponding EVs, at a relatively low dosage, were efficient in promoting the repair of critical-sized osteochondral defects and mitigated the joint inflammation in a rat osteochondral defect model, relative to their normoxia counterpart. In vitro functional test shows enhancement through chondrocyte proliferation, migration, and matrix deposition, while inhibit IL-1β-induced chondrocytes senescence, inflammation, matrix degradation, and pro-inflammatory macrophage activity. Multiple functional proteins, as well as a change in EVs’ size profile, with enrichment of specific EV-miRNAs were detected with hypoxia preconditioning, implicating complex molecular pathways involved in hypoxia pre-conditioned MSCs secretome generated cartilage regeneration. | Yanmeng Yang Yingnan Wu Dahou Yang Shu Hui Neo Nurul Dinah Kadir Doreen Goh Jian Xiong Tan Vinitha Denslin Eng Hin Lee Zheng Yang | 2023 | Bioactive Materials2023，，9： | 0 |
| 20 | Breast imaging:A survey显示文摘Breast cancer is the second leading cause of death in women.It occurs when cells in the breast start to grow out of proportion and invade neighboring tissues or spread throughout the body.Mammography is one of the most effective and popular modalities presently used for breast cancer screening and detection.Efforts have been made to improve the accuracy of breast cancer diagnosis using different imaging modalities.Ultrasound and magnetic resonance imaging have been used to detect breast cancers in high risk patients.Recently,electrical impedance imaging and nuclear medicine techniques are also being widely used for breast cancer screening and diagnosis.In this paper,we discuss the capabilities of various breast imaging modalities. | Subbhuraam Vinitha Sree Eddie Yin-Kwee Ng Rajendra U Acharya Oliver Faust | 2011 | World Journal of Clinical Oncology2011，2，4： | 0 |
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