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| 1 | Extract of buckwheat sprouts scavenges oxidation and inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated macrophages (RAW264.7)显示文摘OBJECTIVE: Buckwheat has been considered as a potential source of nutraceutical components on the world market of probiotic foodstuffs. The purpose of this study was to evaluate the effects of tartary buckwheat (Fagopyrum tataricum) sprouts on oxidation and pro-inflammatory mediators. METHODS: The anti-oxidant effects of buckwheat extract (BWE) and rutin were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-and nitric oxide (NO)-scavenging activities, serum peroxidation and chelating assays. Lipopolysaccharide (LPS)-stimulated RAW264.7 cells were used to evaluate anti-inflammatory activities of buckwheat and rutin. NO production in LPS-stimulated RAW264.7 cells was determined by using Griess reagent. The expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-κB) p65 subunit in cytosolic and nuclear portions were determined by Western blot analysis. Also, the production of inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was determined by enzyme-linked immunosorbent assay. RESULTS: Inhibitory concentration 50 values for DPPHand NO-scavenging activities of BWE were 24.97 and 72.54 μg/mL respectively. BWE inhibited serum oxidation and possessed chelating activity. Furthermore, BWE inhibited IL-6 and TNF-α production in LPS-stimulated RAW264.7 cells. Also, BWE inhibited iNOS and COX-2 expression and NF-κB p65 translocation. CONCLUSION: Buckwheat sprouts possessed strong antioxidant activity and inhibited production of pro-inflammatory mediators in the applied model systems. Thus, buckwheat can be suggested to be beneficial in inflammatory diseases by inhibiting the free radicals and inflammatory mediators. | Rajendra Karki Cheol-Ho Park Dong-Wook Kim | 2013 | Journal of Integrative Medicine2013，11，4： | 17 |
| 2 | Effects of mother tree ages, different rooting mediums, light conditions and auxin treatments on rooting behaviour of Dalbergia sissoo branch cuttings显示文摘Dalbergia sissoo Roxb. is one of the promising multipurpose tree species of South Asia. Most of the plantations of D. sissoo from seeds are facing severe threats due to the die-back disease, which ultimately causes death of this potential tree-species within a few months. Vegetative propagation could avoid the die-back disease. Thirty mother trees of different age-groups of D. sissoo were selected for evaluating the rooting behaviour of branch cuttings from D. sissoo as influenced by auxins (IAA or IBA at 100, 200, 500 mg·L-1), ages of mother trees (10, 4 and 2 years old) and different environment conditions, i.e., different mediums (soil and sand) or light conditions (in shade and open condition). The results show that application of IAA and IBA induced more numbers of cuttings (collected from 10-year-old mother trees) to root compared to control. Branch cuttings of D. sissoo collected from 10-year-old mother trees and planted in soil bed in open conditions had 100.0% of cuttings to root in IAA (100 mg·L-1) and IBA (200 mg·L-1) treatments. Both rooting medium (Soil and sand) influenced significantly (p<0.05) on rooting response of branch cuttings. Soil medium was found to achieve maximum no. of branch cuttings to root, compared to sand medium. | Bhupendra Singh Rajendra Yadav B. P. Bhatt | 2011 | Journal of Forestry Research2011，22，1： | 12 |
| 3 | Multifunctional GelMA platforms with nanomaterials for advanced tissue therapeutics显示文摘Polymeric hydrogels are fascinating platforms as 3D scaffolds for tissue repair and delivery systems of therapeutic molecules and cells.Among others,methacrylated gelatin(GelMA)has become a representative hydrogel formulation,finding various biomedical applications.Recent efforts on GelMA-based hydrogels have been devoted to combining them with bioactive and functional nanomaterials,aiming to provide enhanced physicochemical and biological properties to GelMA.The benefits of this approach are multiple:i)reinforcing mechanical properties,ii)modulating viscoelastic property to allow 3D printability of bio-inks,iii)rendering electrical/magnetic property to produce electro-/magneto-active hydrogels for the repair of specific tissues(e.g.,muscle,nerve),iv)providing stimuli-responsiveness to actively deliver therapeutic molecules,and v)endowing therapeutic capacity in tissue repair process(e.g.,antioxidant effects).The nanomaterial-combined GelMA systems have shown significantly enhanced and extraordinary behaviors in various tissues(bone,skin,cardiac,and nerve)that are rarely observable with GelMA.Here we systematically review these recent efforts in nanomaterials-combined GelMA hydrogels that are considered as next-generation multifunctional platforms for tissue therapeutics.The approaches used in GelMA can also apply to other existing polymeric hydrogel systems. | Amal George Kurian Rajendra KSingh Kapil DPatel Jung-Hwan Lee Hae-Won Kim | 2022 | Bioactive Materials2022，7，2： | 11 |
| 4 | Insect feeding deterrent and growth inhibitory activities of scopoletin isolated from Artemisia annua against Spilarctia obliqua (Lepidoptera: Noctuidae)显示文摘Artemisia annua (Asteraceae ) 由于复合 artemisinin 的存在为它的抗疟药活动是众所周知的。我们从 A 的茎部分孤立 methoxy 香豆素。annua 并且通过质量作为 scopoletin 证实了它的身份光谱数据。结构从 1H-nuclear 被建立磁性的回声(NMR ) , 13C-NMR。复合 scopoletin 为它的喂的挽留的事物和生长被评估对一只有害鳞翅类的昆虫的禁止的潜力, Spilartctia obliqua 沃克。当混合了进人工的饮食时, Scopoletin 给了 FD50 (50% 的喂的挽留的事物) 96.7 g/g 饮食的价值。S。暴露于 scopoletin 的最高的集中(250 g/g 食谱) 的 obliqua 幼虫(12-day-old ) 显示出 77.1% 喂挽留的事物。在生长禁止的试金, scopoletin 在 250 g/g 饮食的最高的剂量向 116.9% 生长抑制提供了 GI50 (50% 的生长抑制) 20.9 g/g 饮食的价值。统计分析向两个都喂威慑和生长显示出一种集中依赖者剂量反应关系禁止的活动。Artemisinin 主要在 A 的叶子被发现。annua 并且不在茎,它典型地作为废物被丢弃。因此在 A 的茎的 scopoletin 的鉴定。annua 可能作为为害虫控制的这材料的来源重要。 | Arun Kumar Tripathi Rajendra Singh Bhakuni Shikha Upadhyay Rashmi Gaur | 2011 | Insect Science2011，18，2： | 10 |
| 5 | Fault Analysis of DC Voltage Dividers in Xiangjiaba--Shanghai ±800 kV UHVDC Project显示文摘 | LI Fengqi SHE Zhengqiu LOU Dianqiang Rajendra Iyer Urban Astrom | 2012 | 高电压技术2012，38，12： | 8 |
| 6 | Underground mining of thick coal seams显示文摘This paper reviews underground mining methods for total thickness of a thick coal seam in single lift(TTTCSSL). Review shows the required engineering for extraction of thick seams needs to be fitted with thickness of the seam, behavior of rock-mass and surrounding stress conditions for efficient mining.Variants of TTTCSSL are able to extract a maximum 10–12 m thickness only. An improvement in bending moment of the overlying coal band in longwall top coal caving(LTCC) provides better under-winning opportunity for the roof coal band. An acceptable limit of 25 MPa compressive strength of coal for the success of LTCC may be increased under favorable geo-technical conditions. Bord and pillar in India adopted induced caving of roof coal band for single lift depillaring of total thickness(SLDTT) of a competent thick coal seam developed along floor. Case studies are given to arrest the adverse effects of extraction height on pillars. | Kumar Rakesh Singh Arun Kumar Mishra Arvind Kumar Singh Rajendra | 2015 | International Journal of Mining Science and Technology2015，25，6： | 7 |
| 7 | Betatrophin:A liver-derived hormone for the pancreatic β-cell proliferation显示文摘The pancreaticβ-cell failure which invariably accompanies insulin resistance in the liver and skeletal muscle is a hallmark of type-2 diabetes mellitus(T2DM).The persistent hyperglycemia of T2DM is often treated with anti-diabetic drugs with or without subcutaneous insulin injections,neither of which mimic the physiological glycemic control seen in individuals with fully functional pancreas.A sought after goal for the treatment of T2DM has been to harness the regenerative potential of pancreaticβ-cells that might obviate a need for exogenous insulin injections.A new study towards attaining this aim was reported by Yi et al,who have characterized a liver-derived protein,named betatrophin,capable of inducing pancreaticβ-cell proliferation in mice.Using a variety of in vitro and in vivo methods,Yi et al,have shown that betatrophin was expressed mainly in the liver and adipose tissue of mice.Exogenous expression of betatrophin in the liver led to dramatic increase in the pancreaticβ-cell mass and higher output of insulin in mice that also concomitantly elicited improved glucose tolerance.The authors discovered that betatrophin was also present in the human plasma.Surprisingly,betatrophin has been previously described by three other names,i.e.,re-feeding-induced fat and liver protein,lipasin and atypical angiopoeitin-like 8,by three independent laboratories,as nutritionally regulated liver-enriched factors that control serum triglyceride levels and lipid metabolism.Yi et al demonstration of betatrophin,as a circulating hormone that regulatesβ-cell proliferation,if successfully translated in the clinic,holds the potential to change the course of current therapies for diabetes. | Rajendra Raghow | 2013 | World Journal of Diabetes2013，4，6： | 6 |
| 8 | Effect of Land Use Change on Runoff and Sediment Yield in Da River Basin of Hoa Binh province, Northwest Vietnam显示文摘The objective of this study was to assess runoff discharge and sediment yield from Da River Basin in the Northwest of Vietnam using Soil and Water Assessment Tools(SWAT) model.The SWAT model was calibrated and validated using the observed monthly stream flows and sediment yield at selected gauging stations.The results indicated that SWAT generally performs well in simulating runoff and sediment yield according to Nash-Sutcliffe efficiency(NSE), Observation's standard deviation ratio(RSR), and percent bias(PBIAS) values.For runoff, the values of NSE, RSR, and PBIAS were 0.98,0.02, and 3.69 during calibration period and 0.99,0.01, and 1.56 during validation period, respectively.For sediment yield, the efficiency was lower than the value of NSE, RSR, and PBIAS during calibration period were 0.81, 0.19, and-4.14 and 0.84, 0.16, and-2.56 during validation period, respectively.The results of the study indicated that the vegetation status has a significant impact on runoff and sediment yield.Changes in land use type between 1995 and2005 from forest to field crop and urban strongly contributed to increasing the average annual runoff from 182.5 to 342.7 mm and sediment yield from101.3 to 148.1 ton-1 ha.Between 2005 and 2010, adecrease of both runoff(from 342.7 to 167.6 mm) and sediment yield(from 148.1 to 74.0 ton-1 ha) was due to the expansion of forested area and application of soil conservation practices.The results of this study are important for developing soil and water conservation programs, extending future SWAT modelling studies and disseminating these results to other regions in Vietnam. | NGO Thanh Son NGUYEN Duy Binh Rajendra Prasad SHRESTHA | 2015 | Journal of Mountain Science2015，12，4： | 6 |
| 9 | Ten mg dexrabeprazole daily is as effective as 20 mg dexrabeprazole daily显示文摘Ten mg dexrabeprazole daily has been shown to be more effective than 20 mg rabeprazole daily against gastroesophageal reflux disease (GERD). This report shows that the efficacy of 10 mg dexrabeprazole daily is equivalent to that of 20 mg dexrabeprazole daily against GERD. This implies that a dose of 10 mg dexra- beprazole is sufficient to block the maximum amount of proton pumps without any need to double the dose as suggested with rabeprazole. | Rajendra Kanakia Suresh Jain | 2008 | World Journal of Gastroenterology2008，14，28： | 6 |
| 10 | Semiconductor metal oxide compounds based gas sensors: A literature review显示文摘 | Sunil Jagannath PATIL Arun Vithal PATIL Chandrakant Govindrao DIGHAVKAR Kashinath Shravan THAKARE Ratan Yadav BORASE Sachin Jayaram NANDRE Nishad Gopal DESHPANDE Rajendra Ramdas AHIRE | 2015 | Frontiers of Materials Science2015，9，1： | 5 |
| 11 | High absorption large-mode area step-index fiber for tandem-pumped high-brightnes shigh-power lasers显示文摘A short absorption length ytterbium(Yb)-doped large-mode area(LMA)fiber is presented as a step forward to mitigate the stern problem of nonlinear scatterings in a tandem pumping scheme adopted for high-power fiber laser.The short absorption length was realized by incorporating high Yb concentration in the fiber core.Furthermore,by replacing the inherent silica cladding with a Ge-doped cladding,we were able to obtain low core numerical aperture(NA)and negate the detrimental effect of index-raising by high Yb concentrations.This overcomes the long-standing limitation in step-index Yb-doped fibers(YDFs)where high cladding absorption inevitably results in high NA,thus hampering single-mode operation.We report an LMA(~575μm^2)YDF with NA of 0.04 and absorption of 27 dB/m at 976 nm both traits promote power scaling of single-mode tandem pumped fiber lasers.To our knowledge,this is the highest cladding absorption attained in a low-NA step-index fiber to date.An all-fiber tandem-pumped amplifier was built using only^14 m of the YDF.The amplifier delivered a near-Gaussian beam(M^i.27)at 836 W output power(pump power limited)with a high slope efficiency of^83%.Thanks to the short length and the tandem pumping,no indication of limiting factors such as stimulated Raman scattering,photodarkening,and transverse mode instability was observed. | KANG-JIE LIM SAMUEL KA-WEN SEAH JOASH YONG'EN YE WENDY WEIYING LIM CHU-PERNG SEAH YUNN-BOON TAN SUTING TAN HUITING LIM RAGHURAMAN SIDHARTHAN ARUMUGAM RAJENDRA PRASADH CHEN-JIAN CHAN SEONGWOO YOO SONG-LIANG CHUA | 2020 | Photonics Research2020，8，10： | 5 |
| 12 | Revisiting the morphology of pelvicalyceal system in human cadaveric kidneys with a systematic review of literature显示文摘Objective:Renal collecting system macroscopically consists of minor calyx,major calyx,renal pelvis and ureter.Stone in renal collecting system is a common presentation in everyday urological practice.The prevalence of renal calculi ranges from 4%to 20%in different geographical distribution.Anatomical variation in renal collecting system plays a significant role in formation of calculi in its parts.The large extra renal pelvis leads to stagnation of urine for longer durations and formation of stones.The stone free rate after percutaneous nephrolithotomy and extra corporeal shock wave lithotomy is significantly related to anatomical factors,particularly the type of renal pelvis and dimensions(length and width)of lower infundibulum.Previous authors described the morphology of pelvicalyceal system in a highly variable manner and the available anatomical description of pelvicalyceal system is contradictory and incomplete.Hence an attempt has been made to provide the precise anatomy of pelvicalyceal system in adult human kidneys.Methods:We studied 196 formalin embalmed kidneys to note the number of infundibulum,major and minor calyces.The location and shape of pelvis were also studied.Results:The intra-renal pelvis was narrow and had funnel shaped appearance in 95 of total 196(48.5%)specimens,and the extra-renal pelvis was dilated as balloon shaped in 43 of 196(21.9%)specimens.In 41(20.9%)specimens,the renal pelvis was having partially intra-and extra-renal in location.Bilateral symmetry was found in only 27.1%of 196 renal collecting systems.The length of lower infundibulum was more than 22 mm in 19(9.7%)of 196 cases which directly affects the stone clearance rate during open and endoscopic surgeries on pelvicalyceal system.Conclusion:This study provides the accurate morphological details of the shape and dimensions of renal pelvicalyceal system to help the urologists and nephrologists. | Kusum Rajendra Gandhi Sushama Chavan | 2019 | Asian Journal of Urology2019，6，3： | 5 |
| 13 | Comparative study of multiple cancellous screws versus sliding hip screws in femoral neck fractures of young adults显示文摘 | Mayank Gupta Rajendra Kumar Arya Satish Kumar Vijay Kumar Jain Skand Sinha Ananta Kumar Naik | 2016 | Chinese Journal of Traumatology2016，19，4： | 5 |
| 14 | Neutrophil-lymphocyte ratio:A prognostic tool in patients with inhospital cardiac arrest显示文摘BACKGROUND In-hospital cardiac arrest(IHCA) portends a poor prognosis and survival to discharge rate. Prognostic markers such as interleukin-6, S-100 protein and high sensitivity C reactive protein have been studied as predictors of adverse outcomes after return of spontaneous circulation(ROSC); however; these variables are not routine laboratory tests and incur additional cost making them difficult to incorporate and less attractive in assessing patient's prognosis. The neutrophil-lymphocyte ratio(NLR) is a marker of adverse prognosis for many cardiovascular conditions and certain types of cancers and sepsis. We hypothesize that an elevated NLR is associated with poor outcomes including mortality at discharge in patients with IHCA.AIM To determine the prognostic significance of NLR in patients suffering IHCA who achieve ROSC.METHODS A retrospective study was performed on all patients who had IHCA with the advanced cardiac life support protocol administered in a large urban community United States hospital over a one-year period. Patients were divided into two groups based on their NLR value(NLR < 4.5 or NLR ≥ 4.5). This cutpoint was derived from receiving operator characteristic curve analysis(area under the curve = 0.66) and provided 73% positive predictive value, 82% sensitivity and42% specificity for predicting in-hospital death after IHCA. The primary outcome was death or discharge at 30 d, whichever came first.RESULTS We reviewed 153 patients with a mean age of 66.1 ± 16.3 years; 48% were female.In-hospital mortality occurred in 65%. The median NLR in survivors was 4.9(range 0.6-46.5) compared with 8.9(0.28-96) in non-survivors(P = 0.001). A multivariable logistic regression model demonstrated that an NLR above 4.55[odds ratio(OR) = 5.20, confidence interval(CI): 1.5-18.3, P = 0.01], older age(OR= 1.03, CI: 1.00-1.07, P = 0.05), and elevated serum lactate level(OR = 1.20, CI:1.03-1.40, P = 0.02) were independent predictors of death.CONCLUSION An NLR ≥ 4.5 may be a useful marker of increased risk of death in patients with IHCA. | Vishal H Patel Philip Vendittelli Rajat Garg Susan Szpunar Thomas LaLonde John Lee Howard Rosman Rajendra H Mehta Hussein Othman | 2019 | World Journal of Critical Care Medicine2019，8，2： | 5 |
| 15 | Treatment of wheat straw using tannase and white-rot fungus to improve feed utilization by ruminants显示文摘Background: Current research to enrich cattle feed has primarily focused on treatment using white rot fungi, while there are scarce reports using the enzyme tannase, which is discussed only in reviews or in the form of a hypothesis. In this context, the aim of the present study was to evaluate the effect of tannase on wheat straw(WS)and also the effect of lyophilized tannase at concentrations of 0.1%, 0.2%, and 0.3%(w/w) on WS followed by fermentation with Ganoderma sp. for 10 d and compared in relation to biochemical parameters, crude protein(CP)content, and nutritional value by calculating the C/N ratio in order to improve the nutritional value of cattle feed.Results: Penicillium chorlesii, a tannase-producing microorganism, produced 61.4 lU/mL of tannase in 54 h when 2%(w/v) tannic acid(TA) was initially used as a substrate in medium containing(% w/v) sucrose(1.0), NaN03(1.0), and MgS〇4(0.08 pH, 5.0) in a 300-L fermentor(working volume 220 L), and concomitantly fed with 1.0%(w/v) TA after24 h. The yield of partially purified and lyophilized tannase was 5.8 lU/mg. The tannin-free myco-straw at 0.1%(w/w) tannase showed 37.8%(w/w) lignin degradation with only a 20.4%(w/w) decrease in cellulose content and the in vitro feed digestibility was 32.2%. An increase in CP content(up to 1.28-fold) along with a lower C/N ratio of25.0%, as compared to myco-straw, was obtained.Conclusions: The use of tannin-free myco-straw has potential to improve the nutritional content of cattle feed.This biological treatment process was safe, eco-friendly, easy to perform, and was less expensive as compared to other treatment methods. | Shailendra Raghuwanshi Swati Misra Rajendra Kumar Saxena | 2014 | Journal of Animal Science and Biotechnology2014，5，2： | 5 |
| 16 | Genetic Relationship and Structure Analysis of Root Growth Angle for Improvement of Drought Avoidance in Early and Mid-Early Maturing Rice Genotypes显示文摘Deeper rooting 1(Dro1)and Deeper rooting 2(Dro2)are the QTLs that contribute considerably to root growth angle assisting in deeper rooting of rice plant.In the present study,a set of 348 genotypes were shortlisted from rice germplasm based on root angle study.Screening results of the germplasm lines under drought stress identified 25 drought tolerant donor lines based on leaf rolling,leaf drying,spikelet fertility and single plant yield.A panel containing 101 genotypes was constituted based on screening results and genotyped using Dro1 and Dro2 markers.Structure software categorized the genotypes into four sub-populations with different fixation index values for root growth angle.The clustering analysis and principal coordinate analysis could differentiate the genotypes with or without deeper rooting trait.The dendrogram constructed based on the molecular screening for deep rooting QTLs showed clear distinction between the rainfed upland cultivars and irrigated genotypes.Eleven genotypes,namely Dular,Tepiboro,Surjamukhi,Bamawpyan,N22,Dinorado,Karni,Kusuma,Bowdel,Lalsankari and Laxmikajal,possessed both the QTLs,whereas 67 genotypes possessed only Dro1.The average angle of Dro positive genotypes ranged from 82.7°to 89.7°.These genotypes possessing the deeper rooting QTLs can be taken as donor lines to be used in marker-assisted breeding programs. | Elssa PANDIT Rajendra Kumar PANDA Auromeera SAHOO Dipti Ranjan PANI Sharat Kumar PRADHAN | 2020 | Rice science2020，27，2： | 4 |
| 17 | Optimizing the seed-cell filling performance of an inclined plate seed metering device using integrated ANN-PSO approach显示文摘Uniformseed distribution within the row is the prime objective of precision planters for better crop growth and yield.Inclined plate planters are generally used for sowing bold seeds likemaize,groundnut,chickpea,and their operating parameters like the forward speed of operation,the seedmetering plate inclination,and the seed level in the hopper affect the cell fill and subsequently the uniformseed distribution.Therefore,to achieve precise seed distribution,these parameters need to be optimized.In the present study,out of the different optimization techniques,a new intelligent optimization technique based on the integrated ANN-PSO approach has been used to achieve the set goal.A 3–5-1 artificial neural network(ANN)model was developed for predicting the cell fill of inclined plate seedmetering device,and the particle swarmoptimization(PSO)algorithmwas applied to obtain the optimum values of the operating parameters corresponding to 100%cell fill.The most appropriate optimal values of the forward speed of operation,the seed metering plate inclination,and the seed level in the hopper for achieving 100%cell fill were found to be 3 km/h,50-degree,and 75%of total height,respectively.The proposed integrated ANN-PSO approach was capable of predicting the optimal values of operating parameters with amaximumdeviation of 2%compared to the experimental results,thus confirmed the reliability of the proposed optimization technique. | C.M.Pareek V.K.Tewari Rajendra Machavaram Brajesh Nare | 2021 | Artificial Intelligence in Agriculture2021，，1： | 4 |
| 18 | 牛脑Ca^(2+)/CaM依赖性蛋白激酶Ⅱ的纯化和鉴定显示文摘通过一系列层析法,首次从牛脑纯化得到胶凝电泳匀一的Ca^(2+)/CaM PKⅡ。凝胶过滤法测定全酶分子量为550kD,SDS-PAGE法测定亚基分子量为55kD,推测牛脑Ca^(2+)/CaM PK Ⅱ由十个相同的亚基组成。该酶活性绝对依赖于Ca^(2+)和CaM,以63kD PDE同工酶为底物,其AC_(50)分别为0.85μmol/L和0.18μmol/L;以酪蛋白为底物,其AC_(50)分别为0.22μmol/L和0.06μmol/L。牛脑Ca^(2+)/CaM PK Ⅱ旣能催化63kD PDE同工酶等多种蛋白或酶磷酸化,又能进行自身磷酸化。该酶催化63kD PDE同工酶最大磷酸参入量为1mol/mol亚基。磷酸化型63kD PDE同工酶的Ca^(2+)的AC_(50)高于非磷酸化型。 | 张光毅 赵昇皓 Rajendra K.Sharma Jerry H.Wang | 1991 | 生物化学杂志1991，7，4： | 4 |
| 19 | 印度中医药发展现状与分析显示文摘印度是位于南亚次大陆的议会共和制国家,其医疗保健工作由州政府负责,拥有世界上最庞大的'免费医疗'服务体系。印度传统医学历史悠久,阿育吠陀(Ayurveda)、自然疗法(Naturopathy)、瑜伽(Yoga)等为其主要代表。公元前2世纪,中医药随宗教交流及贸易活动传入印度并被印度人民熟悉。针灸在印度已取得独立合法地位,其国内拥有多家针灸诊所和针灸协会,但针灸应用仍缺乏系统的行业标准,除针灸外中医药其他疗法在印度尚缺乏发展空间。 | 聂文祎 石晗 Rajendra N.Gadhavi 魏竞竞 赫兰晔 赵静 杨龙会 许家杰 曹洪欣 | 2021 | 国际中医中药杂志2021，43，1： | 4 |
| 20 | hTERT,CEA及CMV启动子在人结肠癌细胞株中的转录活性比较显示文摘目的:比较人端粒酶催化亚单位(hTERT),癌胚抗原(CEA)及巨细胞病毒(CMV)启动子在人结肠癌细胞株LoVo和SW480中的转录活性。方法:设计引物应用PCR法从人结肠癌基因组中克隆hTERT和CEA启动子;用双酶切和PCR法切除原始载体pLVX-EGFP-3FLAG中的CMV启动子后,将hTERT,CEA启动子与该载体重组,构建出重组质粒pLVX-hTERTp-EGFP-3FLAG和pLVX-CEAp-EGFP-3FLAG;将上述两种质粒及原始载体(含CMV启动子)分别瞬时转染人结肠癌细胞株LoVo和SW480后,检测两种细胞株绿色荧光蛋白表达。结果:经PCR,酶切及测序鉴定,克隆及载体构建完全正确。CMV,hTERT及CEA启动子的转录活性(绿色荧光细胞数/总细胞数)在LoVo细胞中依次为54.7%,33.0%,9.5%;在SW480中依次为16.5%,10.1%,8.5%,差异均有统计学意义(均P<0.05)。结论:在人结肠癌细胞株中,转录活性以CMV启动子最高,hTERT启动子次之,CEA启动子最低。该结果可为结肠癌的靶向性基因治疗研究提供参考。 | 贺赛 孙学军 郑见宝 陈南征 任燕飞 Rajendra Gurung 魏光兵 张立 姚建峰 尉春艳 | 2014 | 中国普通外科杂志2014，23，1： | 4 |
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