|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Silybin counteracts lipid excess and oxidative stress in cultured steatotic hepatic cells显示文摘AIM: To investigate in vitro the therapeutic effect and mechanisms of silybin in a cellular model of hepatic steatosis.METHODS: Rat hepatoma Fa O cells were loaded with lipids by exposure to 0.75 mmol/L oleate/palmitate for 3 h to mimic liver steatosis. Then, the steatotic cells were incubated for 24 h with different concentrations(25 to 100 μmol/L) of silybin as phytosome complex with vitamin E. The effects of silybin on lipid accumulation and metabolism, and on indices of oxidative stress were evaluated by absorption and fluorescence microscopy, quantitative real-time PCR, Western blot, spectrophotometric and fluorimetric assays.RESULTS: Lipid-loading resulted in intracellular triglyceride(TG) accumulation inside lipid droplets, whose number and size increased. TG accumulation was mediated by increased levels of peroxisome proliferator-activated receptors(PPARs) and sterol regulatory element-binding protein-1c(SREBP-1c). The lipid imbalance was associated with higher production of reactive oxygen species(ROS) resulting in increased lipid peroxidation, stimulation of catalase activity and activation of nuclear factor kappa-B(NF-κB). Incubation of steatotic cells with silybin 50 μmol/L significantly reduced TG accumulation likely by promoting lipid catabolism and by inhibiting lipogenic pathways, as suggested by the changes in carnitine palmitoyltransferase 1(CPT-1), PPAR and SREBP-1c levels. The reduction in fat accumulation exerted by silybin in the steatotic cells was associated with the improvement of the oxidative imbalance caused by lipid excess as demonstrated by the reduction in ROS content, lipid peroxidation, catalase activity and NF-κB activation.CONCLUSION: We demonstrated the direct antisteatotic and anti-oxidant effects of silybin in steatotic cells, thus elucidating at a cellular level the encouraging results demonstrated in clinical and animal studies. | Giulia Vecchione Elena Grasselli Adriana Voci Francesca Baldini Ignazio Grattagliano David QH Wang Piero Portincasa Laura Vergani | 2016 | World Journal of Gastroenterology2016，22，26： | 13 |
| 2 | Contrast-enhanced sonography versus biopsy for the differential diagnosis of thrombosis in hepatocellular carcinoma patients显示文摘AIM:To clarify which method has accuracy:2nd gen-eration contrast-enhanced ultrasound or biopsy of portal vein thrombus in the differential diagnosis of portal vein thrombosis.METHODS:One hundred and eighty-six patients with hepatocellular carcinoma and portal vein thrombosis underwent in blinded fashion a 2nd generation contrast-enhanced ultrasound and biopsy of portal vein thrombus;both results were examined on the basis of the follow-up of patients compared to reference-standard.RESULTS:One hundred and eight patients completed the study.Benign thrombosis on 2nd generation contrast-enhanced ultrasound was characterised by progressive hypoenhancing of the thrombus;in malignant portal vein thrombosis there was a precocious homo-geneous enhancement of the thrombus.On follow-up there were 50 of 108 patients with benign thrombosis:all were correctly diagnosed by both methods.There were 58 of 108 patients with malignant thrombosis:amongst these,52 were correctly diagnosed by both methods,the remainder did not present malignant cells on portal vein thrombus biopsy and showed on 2nd generation contrast-enhanced ultrasound an inho-mogeneous enhancement pattern.A new biopsy during the follow-up,guided to the area of thrombus that showed up on 2nd generation contrast-enhanced ultra-sound,demonstrated an enhancing pattern indicating malignant cells.CONCLUSION:In patients with hepatocellular carcinoma complicated by portal vein thrombosis,2nd generation contrast-enhanced ultrasound of portal vein thrombus is very useful in assessing the benign or malignant nature of the thrombus.Puncture biopsy of thrombus is usually accurate but presents some sampling errors,so,when pathological results are required,2nd generation contrast-enhanced ultrasound could guide the sampling needle to the correct area of the thrombus. | Paolo Sorrentino Salvatore D'Angelo Luciano Tarantino Umberto Ferbo Alessandra Bracigliano Raffaela Vecchione | 2009 | World Journal of Gastroenterology2009，15，18： | 4 |
| 3 | The Representative Porcine Model for Human Cardiovascular Disease显示文摘 | Yoriyasu Suzuki Alan C. Yeung Fumiaki Ikeno Andrea Vecchione | 2010 | Journal of Biomedicine and Biotechnology2010，，： | 2 |
| 4 | Rodent Models for Metabolic Syndrome Research显示文摘 | Sunil K. Panchal Lindsay Brown Andrea Vecchione | 2010 | Journal of Biomedicine and Biotechnology2010，，： | 2 |
| 5 | Animal Models of Colitis-Associated Carcinogenesis显示文摘 | Manasa Kanneganti Mari Mino-Kenudson Emiko Mizoguchi Andrea Vecchione | 2011 | Journal of Biomedicine and Biotechnology2011，，： | 2 |
| 6 | Antitumor activity of pimasertib, a selective MEK 1/2 inhibitor, in combination with PI3K/mTOR inhibitors or with multi‐targeted kinase inhibitors in pimasertib‐resistant human lung and colorectal cancer cells显示文摘 | Erika Martinelli Teresa Troiani Elena D’Aiuto Floriana Morgillo Donata Vitagliano Anna Capasso Sarah Costantino Loreta Pia Ciuffreda Francesco Merolla Loredana Vecchione Veerle Vriendt Sabine Tejpar Anna Nappi Vincenzo Sforza Giulia Martini Liberato Berri | 2013 | Int J Cancer2013，，9： | 2 |
| 7 | A kiwifruit (Actinidia spp.) linkage map based on microsatellites and integrated with AFLP markers显示文摘 | R. Testolin W. G. Huang O. Lain R. Messina A. Vecchione G. Cipriani | 2001 | TAG Theoretical and Applied Genetics2001，，1： | 2 |
| 8 | Cervical dysptasia, ploidy, and human papillomavirus status comelate with loss of FHIT expression显示文摘 | Vecchione A Zanesi N Trombettaq | 2001 | Clin Cancer Res2001，7，5： | 1 |
| 9 | Cancer prevention and therapy in a preclinical mouse model: impact of FHIT viruses显示文摘 | Ishii H Vecchione A Fong LY | 2004 | Curr Gcnc Thcr2004，4，1： | 1 |
| 10 | Specific microRNAs are downregulated in human thyroid anaplastic carcinomas 显示文摘 | Visone R Pallante P Vecchione A | 2007 | Oncogene2007，26，54： | 1 |
| 11 | Specific microRNAs are downregulated in human thyroid anaplastic carcinomas显示文摘 | Visone R Pallante P Vecchione A | | 0，，： | 1 |
| 12 | Effect of adenoviral transduction of the fragile histidine triad gene into esophageal cancer cells显示文摘 | Ishii H Dumon KR Vecchione A | 2001 | Cancer Res2001，61，4： | 1 |
| 13 | Expression of FRA16D/WWOX and FRA3B/FHIT genes in hematopoietic malignancies显示文摘 | Ishii H Vecchione A Furukawa Y | 2003 | Mol Cancer Res2003，1，13： | 1 |
| 14 | Leptin effect on en- dothelial nitric oxide is mediated through Aket-endothe- lial nitric oxide sythase phosphorylation pathway 显示文摘 | Vecchione C Maffei A Colella S | 2002 | Di- abetes2002，51，1： | 1 |
| 15 | Separation of omphalopagus twins: unigue reconstruction using syngeneic cryopreserved tissue显示文摘 | Canty TG Sr Mainwaring R Vecchione T | 1998 | J Pediatr Surg1998，33，5： | 1 |
| 16 | HPV infection and microsatellite instability in squamous lesions of the uterine cervix显示文摘 | French D Cermele C Vecchione A | 2000 | Anticancer Res2000，20，5： | 1 |
| 17 | Leptin effect on endothelial nitricoxide is mediated through Akt-endothelial nitricoxidesynthase phosphorylation pathway显示文摘 | Vecchione C Maffei A Colella S | 2002 | Diabetes2002，51，1： | 1 |
| 18 | Specific mi- croRNAs are downregulated in human thyroid anaplastic carcino- mas显示文摘 | VISONE R PALIANTE P VECCHIONE A | 2007 | Oncogene2007，26，54： | 1 |
| 19 | Fragile histidine triad expression delays tumor development and induces apoptosis in human pancreatic cancer 显示文摘 | Dumon KR Ishii H Vecchione A | 2001 | Cancer Res2001，61，12： | 1 |
| 20 | Emilinl links TGF-beta maturation to blood pressure homeostasis 显示文摘 | ZACCHIGNA L VECCHIONE C NOTRE A | 2006 | Cell2006，124，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员