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| 1 | Silybin counteracts lipid excess and oxidative stress in cultured steatotic hepatic cells显示文摘AIM: To investigate in vitro the therapeutic effect and mechanisms of silybin in a cellular model of hepatic steatosis.METHODS: Rat hepatoma Fa O cells were loaded with lipids by exposure to 0.75 mmol/L oleate/palmitate for 3 h to mimic liver steatosis. Then, the steatotic cells were incubated for 24 h with different concentrations(25 to 100 μmol/L) of silybin as phytosome complex with vitamin E. The effects of silybin on lipid accumulation and metabolism, and on indices of oxidative stress were evaluated by absorption and fluorescence microscopy, quantitative real-time PCR, Western blot, spectrophotometric and fluorimetric assays.RESULTS: Lipid-loading resulted in intracellular triglyceride(TG) accumulation inside lipid droplets, whose number and size increased. TG accumulation was mediated by increased levels of peroxisome proliferator-activated receptors(PPARs) and sterol regulatory element-binding protein-1c(SREBP-1c). The lipid imbalance was associated with higher production of reactive oxygen species(ROS) resulting in increased lipid peroxidation, stimulation of catalase activity and activation of nuclear factor kappa-B(NF-κB). Incubation of steatotic cells with silybin 50 μmol/L significantly reduced TG accumulation likely by promoting lipid catabolism and by inhibiting lipogenic pathways, as suggested by the changes in carnitine palmitoyltransferase 1(CPT-1), PPAR and SREBP-1c levels. The reduction in fat accumulation exerted by silybin in the steatotic cells was associated with the improvement of the oxidative imbalance caused by lipid excess as demonstrated by the reduction in ROS content, lipid peroxidation, catalase activity and NF-κB activation.CONCLUSION: We demonstrated the direct antisteatotic and anti-oxidant effects of silybin in steatotic cells, thus elucidating at a cellular level the encouraging results demonstrated in clinical and animal studies. | Giulia Vecchione Elena Grasselli Adriana Voci Francesca Baldini Ignazio Grattagliano David QH Wang Piero Portincasa Laura Vergani | 2016 | World Journal of Gastroenterology2016，22，26： | 13 |
| 2 | Prevalence and clinical significance of acquired left coronary artery fistulas after surgical myectomy in patients with hypertrophic cardiomyopathy显示文摘 | Aurelio Sgalambro Iacopo Olivotto Alessandra Rossi Stefano Nistri Katia Baldini Massimo Baldi Pierluigi Stefano David Antoniucci Francesca Garbini Franco Cecchi Magdi H. Yacoub | 2010 | The Journal of Thoracic and Cardiovascular Surgery2010，，5： | 2 |
| 3 | Hepatocellular Carcinomas and Primary Liver Tumors as Predictive Factors for Postoperative Mortality after Liver Resection: A Meta-Analysis of More than 35,000 Hepatic Resections显示文摘 | Ramacciato Giovanni D’Angelo Francesco Baldini Rossella Petrucciani Niccoló Antolino Laura Aurello Paolo Nigri Giuseppe Bellagamba Riccardo Pezzoli Francesca Balesh Albert Cucchetti Alessandro Cescon Matteo Del Gaudio Massimo Ravaioli Matteo | 2012 | EN2012，，4： | 2 |
| 4 | Clinical Features and Outcome of Hypertrophic Cardiomyopathy Associated With Triple Sarcomere Protein Gene Mutations显示文摘 | Francesca Girolami Carolyn Y. Ho Christopher Semsarian Massimo Baldi Melissa L. Will Katia Baldini Francesca Torricelli Laura Yeates Franco Cecchi Michael J. Ackerman Iacopo Olivotto | 2010 | Journal of the American College of Cardiology2010，，14： | 1 |
| 5 | Insulin receptor isoforms are differently expressed during human osteoblastogenesis显示文摘 | Sofia Avnet Francesca Perut Manuela Salerno Laura Sciacca Nicola Baldini | 2012 | Differentiation2012，，5： | 1 |
| 6 | Clinical Features and Outcome of Hypertrophic Cardiomyopathy Associated With Triple Sarcomere Protein Gene Mutations显示文摘 | Francesca Girolami Carolyn Y. Ho Christopher Semsarian Massimo Baldi Melissa L. Will Katia Baldini Francesca Torricelli Laura Yeates Franco Cecchi Michael J. Ackerman Iacopo Olivotto | 2010 | Journal of the American College of Cardiology2010，，14： | 1 |
| 7 | Osteoblasts from a mandibuloacral dysplasia patient induce human blood precursors to differentiate into active osteoclasts显示文摘 | Sofia Avnet Rosanna Pallotta Francesca Perut Nicola Baldini Maria Gabriela Pittis Anita Saponari Enrico Lucarelli Barbara Dozza Tiziana Greggi Nadir M. Maraldi Cristina Capanni Elisabetta Mattioli Marta Columbaro Giovanna Lattanzi | 2011 | BBA - Molecular Basis of Disease2011，，7： | 1 |
| 8 | Distal intramural spread in adenocarcinoma of the lower third of the rectum treated with total rectal resection and coloanal anastomosis显示文摘 | Salvatore Andreola M.D. Ermanno Leo M.D. Filiberto Belli M.D. Cinzia Lavarino Ph.D. Rosaria Bufalino Gorana Tomasic M.D. Maria Teresa Baldini M.D. Francesca Valvo M.D. Pierina Navarria M.D. Fabrizio Lombardi M.D | 1997 | Diseases of the Colon & Rectum1997，，1： | 1 |
| 9 | Prevalence and incidence of depressive and anxious symptoms in couples undergoing assisted reproductive treatment in an Italjan infertility department 显示文摘 | FRANCESCA CHIAFFARINO MARIA P BALDINI | 2011 | Eur J Obstet Gynecol Reprod Bio2011，158，2： | 1 |
| 10 | Clinical Features and Outcome of Hypertrophic Cardiomyopathy Associated With Triple Sarcomere Protein Gene Mutations显示文摘 | Francesca Girolami Carolyn Y. Ho Christopher Semsarian Massimo Baldi Melissa L. Will Katia Baldini Francesca Torricelli Laura Yeates Franco Cecchi Michael J. Ackerman Iacopo Olivotto | 2010 | Journal of the American College of Cardiology2010，，14： | 1 |
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