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| 1 | DK crush technique: modified treatment of bifurcation lesions in coronary artery显示文摘 | CHEN Shao-liang YE Fei ZHANG Jun-jie ZHU Zhong-sheng LIN Song SHAN Shou-jie LIU Zhi-zhong LIU Yan DUAN Bao-xiang GE Jun-bo | 2005 | Chinese Medical Journal2005，，20： | 35 |
| 2 | Impact of postoperative omega-3 fatty acid-supplemented parenteral nutrition on clinical outcomes and immunomodulations in colorectal cancer patients显示文摘AIM: To investigate the effect of omega-3 fatty acid parenteral supplementation postoperatively on clinical outcomes and immunomodulation in colorectal cancer patients. METHODS: Forty-two patients undergoing radical colorectal cancer resection with an indication for total parenteral nutrition postoperatively were enrolled in this prospective, double-blind, randomized, controlled study. Patients received total parenteral nutrition supplemented with either soybean oil (LCT; Intralipid, Fresenius-Kabi, SO group, n = 21) or a combination of omega-3 fish oil and soybean oil (LCT:fish oil = 5:1, fish oil; Omegaven, Fresenius-Kabi, FO group, n = 21), up to a total of 1.2 g lipid/kg per day for 7 d postoperatively. A same volume calorie and nitrogen was administrated. Routine blood test, biochemistry, systemic levels of IL-6 and TNF-α, percentage of CD3+, CD4+, and CD8+ lymphocytes were evaluated preoperatively and on postoperative d 1 and 8. Patient outcome was evaluated considering mortality during the hospital stay, length of postoperative hospital stay, and occurrence of infectious complications. RESULTS: Both lipid regimens were well tolerated. No differences between the two groups were noticed in demographics, baseline blood test, biochemistry, serum levels of IL-6 and TNF-α, percentage of CD4+, CD8+ lymphocytes, and ratios of CD4+/CD8+. Compared with those on postoperative d 1, serum IL-6 levels onpostoperative d 8 were significantly depressed in the FO group than in the reference group (-44.43 ± 30.53 vs -8.39 ± 69.08, P = 0.039). Simultaneously, the ratios of CD4+/CD8+ were significantly increased in the FO group (0.92 ± 0.62 vs 0.25 ± 1.22, P = 0.035). In addition, depression of serum TNF-α levels (-0.82 ± 2.71 vs 0.27 ± 1.67, P = 0.125) and elevation of CD3+ and CD4+ lymphocyte percentage (12.85 ± 11.61 vs 3.84 ± 19.62, P = 0.081, 17.80 ± 10.86 vs 9.66 ± 17.55, P = 0.084, respectively) were higher in the FO group than in the reference group. Patients in the FO group trended to need a shorter postoperative hospital stay (17.45 ± 4.80 d vs 19.62 ± 5.59 d, P = 0.19). No statistically significant difference was found when stratified to mortality and occurrence of infectious complications. CONCLUSION: Postoperative supplementation of omega-3 fatty acids may have a favorable effect on the outcomes in colorectal cancer patients undergoing radical resection by lowering the magnitude of inflammatory responses and modulating the immune response. | Bin Liang, Shan Wang, Ying-Jiang Ye, Xiao-Dong Yang, You-Li Wang, Jun Qu, Qi-Wei Xie, Mu-Jun Yin, Division of Surgical Oncology and Division of Gastroenterological Surgery, Peking University People’s Hospital, Beijing 100044, China Author contributions: Liang B and Wang S contributed equally to this work Liang B and Wang S designed the research Liang B, Ye YJ, Yang XD, Wang YL, Qu J, Xie QW, Yin MJ performed the research and collected the data Wang S and Ye YJ supervised the research Liang B and Ye YJ analyzed the data Liang B wrote the paper and revised the manuscript. | 2008 | World Journal of Gastroenterology2008，14，15： | 31 |
| 3 | Non-canonical regulation of SPL transcription factors by a human OTUBl-like deubiquitinase defines a new plant type rice associated with higher grain yield显示文摘完成增加的谷物生产率长是引起节目的谷物的制服的焦点。ideotype 途径被用来在国际瑞斯研究院并且在中国改进米饭收益潜力。然而,在米饭的收益相关的特点的基因基础仍然保持不清楚。这里,我们显示出那一个主要量的特点地点, qNPT1,通过建筑学由更少 tillers 描绘了的一种新植物类型(NPT ) 的决心的行为,更坚定的灰煤杆和更大的圆锥花序,并且它与相同编码 deubiquitinating 酶到人的 OTUB1。OsOTUB1 的 Downregulation 提高 meristematic 活动,导致在谷物产量的减少的 tiller 数字,增加的谷物数字,提高的谷物重量和米饭的作为结果的增加。不同于人的 OTUB1, OsOTUB1 能劈开连接 K48 、连接 K63 的 polyubiquitin。OsOTUB1 与 E2 结合 ubiquitin 蛋白质 OsUBC13 和 squamosa 倡导者绑定交往像蛋白质的抄写因素 OsSPL14。OsOTUB1 和 OsSPL14 分享普通目标基因,和他们的物理相互作用限制 OsSPL14 的连接 K63 的 ubiquitination (K63Ub ) ,它接着支持 OsSPL14 的 K48Ub 依赖的 proteasomal 降级。相反地, OsOTUB1 的 loss-of-function 与 OsSPL14 的高水平的累积被相关,导致 NPT 建筑学。我们也证明 high-yielding npt1 和 dep1-1 等位基因节节上升为增加在当前是可完成的的上面的米饭收益潜力提供新策略。 | Shuansuo Wang Kun Wu Qian Qian Qian Liu Qi Li Yajun Pan Yafeng Ye Xueying Liu Jing Wang Jianqing Zhang Shan Li Yuejin Wu Xiangdong Fu | 2017 | Cell Research2017，27，9： | 33 |
| 4 | Identification of a natural mutant of HBV X protein truncated 27 amino acids at the COOH terminal and its effect on liver cell proliferation显示文摘目的:识别肝炎 B ( HBV ) X ( HBx )基因的异种并且在肝房间 proliferation.Methods 上调查自然异种的效果:我们从 188 重量的单位识别了 HBx 基因的自然异种一中国病人的 48 纸巾由 PCR 与 HBV 感染了的and , HBx 基因的异种的鉴定上的 respectively.Based ,我们克隆异种的碎片进 pcDNA3 向量。异种的生物活动是 investigated.Results : 我们从 3 重量的单位从 382 ~ 401 基础对与删除识别了 HBx 基因的自然异种一 out of 188 个病人,它在 COOH 终端从第 128 氨基酸导致了 HBx 蛋白质的表达式删除。有从在 COOH 终端的 382 基础对的删除的类似的异种从 48 肝细胞癌纸巾从染色体的 5 个案例被识别。关于异种的生物活动,我们发现短暂 transfection 没有通过到 induce apoptosis,但是支持了人的肝的增长的 HBx 蛋白质的异种由稳定的 transfection 使 L-02 房间不朽,与野类型的 HBx 蛋白质相比。数据证明变异的 stably-trans-fected L-02-X-Sera 房间和碎片 stably-transfected L-02-XA127 房间的增长被 BrdU 加入试金提高,流动血细胞计数 analysis.Lu-ciferase 记者基因试金证明颠倒 transcriptase 是起来的 NF-KB , survivin ,和人的 telomerase 的 transcriptional 活动调整了,并且西方的污点分析表明原子抗原( PCNA )是起来的 c-Myc 和增殖的房间的表达式层次在 cells.Conclusion 调整了:我们的调查结果建议自然 HBx 异种在 COOH 终端截断了 27 氨基酸支持房间增长。 | Hang ZHANG Chang-liang SHAN Nan LI Xuan ZHANG Xue-zhi ZHANG Fu-qing XU Shuai ZHANG Li-yan QIU Li-hong YE Xiao-dong ZHANG | 2008 | Acta Pharmacologica Sinica2008，29，4： | 28 |
| 5 | Aphasia rehabilitation based on mirror neuron theory: a randomized-block-design study of neuropsychology and functional magnetic resonance imaging显示文摘When watching someone performs an action, mirror neurons are activated in a way that is very similar to the activation that occurs when actually performing that action. Previous single-sample case studies indicate that hand-action observation training may lead to activation and remodeling of mirror neuron systems, which include important language centers, and may improve language function in aphasia patients. In this randomized-block-design experiment, we recruited 24 aphasia patients from, Zhongda Hospital, Southeast University, China. The patients were divided into three groups where they underwent hand-action observation and repetition, dynamic-object observation and repetition, or conventional speech therapy. Training took place 5 days per week, 35 minutes per day, for 2 weeks. We assessed language function via picture naming tests for objects and actions and the Western Aphasia Battery. Among the participants, one patient, his wife and four healthy student volunteers underwent functional magnetic resonance imaging to analyze changes in brain activation during hand-action observation and dynamic-object observation. Results demonstrated that, compared with dynamic-object observation, hand-action observation led to greater performance with respect to the aphasia quotient and affiliated naming sub-tests and a greater Western Aphasia Battery test score. The overall effect was similar to that of conventional aphasia training, yet hand-action observation had advantages compared with conventional training in terms of vocabulary extraction and spontaneous speech. Thus, hand-action observation appears to more strongly activate the mirror neuron system compared with dynamic-object observation. The activated areas included Broca's area, Wernicke's area, and the supramarginal gyrus. These results suggest that hand-action observation combined with repetition might better improve language function in aphasia patients compared with dynamic-object observation combined with repetition. The therapeutic mechanism of this intervention may be associated with activation of additional mirror neuron systems, and may have implications for the possible repair and remodeling of damaged nerve networks. The study protocol was approved by the Ethical Committee of Nanjing Medical University, China(approval number: 2011-SRFA-086) on March 11, 2011. This trial has been registered in the ISRCTN Registry(ISRCTN84827527). | Wen-Li Chen Qian Ye Si-Cong Zhang Yang Xia Xi Yang Ti-Fei Yuan Chun-Lei Shan Jian-An Li | 2019 | Neural Regeneration Research2019，14，6： | 28 |
| 6 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 7 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 8 | Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds显示文摘最近,我们报导了一条化学途径从老鼠成纤维细胞产生 pluripotent 干细胞。然而,是否化学上导致了 pluripotent 干细胞(CiPSCs ) ,能从要示威的另外的房间类型遗体被导出。用系跟踪,这里,我们首先从成纤维细胞验证 CiPSCs 的产生。下次,我们证明从外胚层的神经干细胞(NSC ) 和从内胚叶的小肠的上皮的房间(IEC ) 能是化学上 reprogrammed 进 pluripotent 干细胞。CiPSCs 源于 NSC 和 IEC 类似于老鼠处于增长率,全球基因表示侧面, epigenetic 地位,自强和区别能力的胚胎的干细胞,和 germline 传播才能。有趣地, pluripotency 基因 Sall4 从不同房间类型,和一样的核心在化学 reprogramming 过程在起始的阶段被表示小分子为 reprogramming 被要求,在从这些多样的房间类型位于化学 reprogramming 下面的分子的机制建议保存。我们的分析也证明这些小分子的使用应该被调整从不同房间类型满足 reprogramming 的要求。一起,这些调查结果证明那条完整的化学 reprogramming 途径能在不同织物起源的房间被使用并且建议化学 reprogramming 是有要延长到更起始的类型的潜力的有希望的策略。 | Junqing Ye Jian Ge Xu Zhang Lin Cheng Zhengyuan Zhang Shan He Yuping Wang Hua Lin Weifeng Yang Junfang Liu Yang Zhao Hongkui Deng | 2016 | Cell Research2016，26，1： | 19 |
| 9 | Impact of multidisciplinary team working on the management of colorectal cancer显示文摘背景被用于诊断和治疗的技术的不断、快速的发展使 colorectal 癌症的管理更困难取决于单个 discipline.Colorectal 癌症工作的多学科的队( MDT )模型被英国和另外的国家推荐,但是几乎在这研究的 China.The 目的研究 colorectal 癌症的管理的 MDT 的影响上没有很少信息是在 MDT.Meth 的开始以后在 colorectal | Ye Ying-jiang Shen Zhan-long Sun Xian-tao Wang Zhi-feng Shen Dan-hua Liu Hui-jun Zhang Wan-lei Chen Ya-lin Zhou Jing Poston, G. J. Wang Shan | 2012 | Chinese Medical Journal2012，，2： | 17 |
| 10 | “Omics” in pharmaceutical research: overview, applications, challenges, and future perspectives显示文摘In the post-genomic era, biological studies are characterized by the rapid development and wide application of a series of 'omics' technologies, including genomics, proteomics, metabolomics, transcriptomics, lipidomics, cytomics, metallomics, ionomics, interactomics, and phenomics. These 'omics' are often based on global analyses of biological samples using high through-put analytical approaches and bioinformatics and may provide new insights into biological phenomena. In this paper, the development and advances in these omics made in the past decades are reviewed, especially genomics, transcriptomics, proteomics and metabolomics; the applications of omics technologies in pharmaceutical research are then summarized in the fields of drug target discovery, toxicity evaluation, personalized medicine, and traditional Chinese medicine; and finally, the limitations of omics are discussed, along with the future challenges associated with the multi-omics data processing, dynamics omics analysis, and analytical approaches, as well as amenable solutions and future prospects. | YAN Shi-Kai LIU Run-Hui JIN Hui-Zi LIU Xin-Ru YE Ji SHAN Lei ZHANG Wei-Dong | 2015 | Chinese Journal of Natural Medicines2015，13，1： | 16 |
| 11 | Recurrent gain-of-function USP8 mutations in Cushing's disease显示文摘库欣的疾病,引起过量皮质醇生产的也已知的同样促肾上腺皮质的荷尔蒙(ACTH )-secreting 垂体腺瘤(舞步) ,占多达 85%corticotrophin 依赖的库欣的症候群盒子。然而,在这疾病的基因改变是不清楚的。这里,我们执行了从 12 秘密 ACTH 舞步导出并且匹配血样品的 DNA 定序的 whole-exome,它在候选人基因揭示了体的变化的三种类型, USP8 (编码 ubiquitin 特定的朊酶 8 ) ,专门在 exon 14 在 12 秘密 ACTH 舞步中的 8 个。我们进一步评估了体的 USP8 变化在另外由定序的 Sanger 的 258 舞步。进一步定序指向在 108 秘密 ACTH 舞步(62.04%) 中的 67 个识别了 USP8 变体的 17 种类型的一个总数。然而,任何一个都没在舞步的另外的类型这些变化被检测(n = 150 ) 。这些变化在 USP8 的 14-3-3 绑定主题以内聚集并且破坏在 USP8 和 14-3-3 蛋白质之间的相互作用,导致保护 EGFR 免受 lysosomal 降级的伤害的一个提高的能力。因此,有 EGFR 表示,提高的 EGFR 蛋白质丰富和 mRNA 表示的更高的发生 POMC 铺平的变异的 USP8 显示的舞步,它编码 ACTH 的先锋。有变异的 USP8 的舞步在尺寸是显著地更小的并且比野类型的舞步有更高的 ACTH 生产。在里面通过手术 resected 主要变异 USP8 的肿瘤房间, USP8 击倒或堵住的 EGFR 有效地稀释 ACTH 分泌物。一起拿,体的 gain-of-function USP8 变化是普通的并且在库欣的疾病贡献 ACTH 生产过剩。USP8 或 EGFR 的抑制为对待变异 USP8 的 corticotrophin 腺瘤是有希望的。我们的学习在库欣的疾病加亮潜在地功能的变异的基因并且提供卓见进这疾病的治疗学。 | Zeng-Yi Ma Zhi-Jian Song Jian-Hua Chen Yong-Fei Wang Shi-Qi Li Liang-Fu Zhou Ying Mao Yi-Ming Li Rong-Gui Hu Zhao-Yun Zhang Hong-Ying Ye Ming Shen Xue-Fei Shou Zhi-Qiang Li Hong Peng Qing-Zhong Wang Dai-Zhan Zhou Xiao-Lan Qin Jue Ji Jie Zheng Hong Chen Yin Wang Dao-Ying Geng Wei-Jun Tang Chao-Wei Fu Zhi-Feng Shi Yi-Chao Zhang Zhao Ye Wen-Qiang He Qi-Lin Zhang Qi-Sheng Tang Rong Xie Jia-Wei Shen Zu-Jia Wen Juan Zhou Tao Wang Shan Huang Hui-Jia Qiu Ni-Dan Qiao Yi Zhang Li Pan Wei-Min Bao Ying-Chao Liu Chuan-Xin Huang Yong-Yong Shi Yao Zhao | 2015 | Cell Research2015，25，3： | 16 |
| 12 | EZH2 and STAT6 expression profiles are correlated with colorectal cancer stage and prognosis显示文摘AIM: To investigate the role of enhancer of zeste homologue 2 (EZH2) and STAT6 immunohistochemistry in the evaluation of clinical stages and prognosis of colorectal cancer (CRC). METHODS: The expression patterns were examined by immunohistochemistry in both tumor and adjacent non-neoplastic tissues of 119 CRC patients who underwent operation during the time period from 2002 to 2004. RESULTS: The positive rates of EZH2 and STAT6 in CRC cases were 69.7% (83 of 119) and 60.5% (72 of 119), respectively, and there was signifi cant differ-ence when compared with tumor adjacent non-neoplastic tissues (P < 0.05). In all CRC cases, patientswith EZH2-positive, or STAT6-positive expression had lower survival rates than those with EZH2-negative or STAT6-negative expression (P = 0.002 and P = 0.005, respectively). Co-expression of EZH2 and STAT6 showed signifi cantly higher levels in CRC cases of high clinical TNM stages (P = 0.001), and the expression of STAT6 was also correlated with lymph node metastasis and distant metastasis (P = 0.001 and P = 0.016, respectively). Multivariate analysis revealed that EZH2 expression was an independent prognostic indicator of CRC (P = 0.039). CONCLUSION: EZH2 and STAT6 expressions have significant values in distinguishing clinical stages of CRC and predicting the prognosis of the patients. | Wang, Cheng-Gang Ye, Ying-Jiang Yuan, Jing Liu, Fang-Fang Zhang, Hui Wang, Shan | 2010 | World Journal of Gastroenterology2010，16，19： | 16 |
| 13 | Hepatitis B virus X protein promotes liver cell proliferation via a positive cascade loop involving arachidonic acid metabolism and p-ERK1/2显示文摘肝炎 B 病毒 X 蛋白质(HBx ) 在 hepatocellular 癌的发展起一个关键作用。这里,我们寻求了识别 HBx 由调停的机制肝房间增长。我们发现那 HBx upregulated cyclooxygenase-2 (COX-2 ) 的层次, 5-lipoxygenase (5 哈鱼) 和 phosphorylated 在肝房间的细胞外的调整信号的蛋白质 kinases 1/2 (p-ERK1/2 ) 。导致 HBx 的 p-ERK1/2 被 Gi/o 蛋白质,艇长或哈鱼的抑制废除。另外, HBx 增加了前列腺素 E2 (PGE2 ) 的数量,免除房间线的 leukotriene B4 (LTB4 ) 源于 hepatocytes。而且,这些释放 arachidonic 酸代谢物能激活 ERK1/2。有趣地,激活 ERK1/2 能 upregulate 以一种积极反馈方式的 COX-2 和 5 哈鱼的表示。在结论, HBx 经由包含 COX-2, 5 哈鱼,释放 arachidonic 酸代谢物, Gi/o 蛋白质和 p-ERK1/2 的一个积极反馈环提高并且维持肝房间增长。 | Changliang Shan Fuqing Xu Shuai Zhang Jiacong YOU Xiaona You Liyan Qiu Jie Zheng Lihong Ye Xiaodong Zhang | 2010 | Cell Research2010，20，5： | 15 |
| 14 | NF-κB downregulation may be involved the depression of tumor cell proliferation mediated by human mesenchymal stem cells显示文摘目的: 干细胞对到肿瘤发生的家有能力并且禁止肿瘤房间的增长,这被报导了。我们的学习的目的是表明肝细胞瘤房间的禁止的增长的分子的机制,乳癌房间由人的间充质的干细胞( hMSCs )调停了 .Methods : H7402 人的肝细胞瘤房间和 MCF-7 人的增长乳癌房间被 5-bromodeoxyuridine ( BrdU )测量加入试金并且流动在有从 hMSCs 文化的调节媒介的治疗以后的血细胞计数试金,例如 Z3 房间或 BMMS-03 房间。 NF-KAPPA B 的角色或禁止者 kappa B 高山的磷酸化哈( p-I kappa B 高山哈)在从 Z3 与调节媒介对待的肝细胞瘤或乳癌房间的消沉,房间或 BMMS-03 房间被记者基因试金,量的即时 PCR ,和西方的污点分析检验, respectively.Results : H7402 房间和 MCF-7 房间的增长被 BrdU 加入试金和流动血细胞计数试金术后疗法显著地减少。transcriptional 活动和 NF-KAPPA B 的 mRNA 水平低以一种剂量依赖者方式由记者基因试金和量的实时 PCR 在对待的房间调整了。在蛋白质水平, NF-KAPPA B 和 p-lKBoc 由西方的污点 analysis.Conclusion 在对待的房间减少了: 从 hMSCs 的调节媒介能在规定下面禁止肿瘤 cells.NF-KAPPA B 的增长为肿瘤房间增长的消沉的原因之一被 hMSCs 调停。 | Ling QIAO Tie-jun ZHAO Feng-ze WANG Chang-liang SHAN Li-hong YE Xiao-dong ZHANG | 2008 | Acta Pharmacologica Sinica2008，29，3： | 12 |
| 15 | Multi-step formation, evolution, and functionalization of new cytoplasmic male sterility genes in the plant mitochondrial genomes显示文摘新基因起源是授与 phenotypic 变化和生物差异的 genomic 革新的主要来源。在植物的新 mitochondrial 基因的产生可以引起细胞质的男绝育(厘米) ,它能支持 outcrossing 和增加健康。然而, mitochondrial 基因怎么在结构和功能发源并且演变,仍然保持不清楚。米饭厘米的野未成功的类型被 mitochondrial 基因 WA352c (以前命名的 WA352 ) 授与并且广泛地在混合米饭繁殖被利用了。这里,我们重建由 11 mitochondrial genomic recombinant 的鉴定和分析的 WA352c 的进化轨道在野、栽培的米饭与 WA352c 有关组织。我们推断这些结构在野米饭 Oryza rufipogon,结合的 substoichiometric 变和顺序变化的 mitochondrial 染色体在保存 mitochondrial 序列之中通过多重重新整理产生了。我们鉴别二表示了,但是在这些结构之中的 nonfunctional protogenes,和他们能发展进功能的厘米基因经由的表演定序能减轻蛋白质的自我禁止的潜力的变化。这些顺序变化将资助蛋白质与编码原子核的 mitochondrial 蛋白质 COX11 交往的能力,在花药 tapetum 和男绝育导致早熟的规划房间死亡。而且,我们证明把 COX11 相互作用领域编码为这些 WA352c 相关的基因的序列经历了在进化期间净化选择。我们经由在结构,顺序,拷贝数字,和功能包含渐渐的变化的 multi-recombination/protogene formation/functionalization 机制为新厘米基因的形成和进化建议一个模型。 | Huiwu Tang Xingmei Zheng Chuliang Li Xianrong Xie Yuanling Chen Letian Chen Xiucai Zhao Huiqi Zheng Jiajian Zhou Shan Ye Jingxin Guo Yao-Guang Liu | 2017 | Cell Research2017，27，1： | 11 |
| 16 | Hangzhou criteria as downstaging criteria in hepatocellular ca显示文摘Background:The downstaging of hepatocellular carcinoma(HCC)has been confirmed to benefit liver transplantation(LT)patients whose tumors are beyond the transplantation criteria.Milan criteria(MC),a tumor size and number-based assessment,is currently used as the endpoint in these patients.However,many studies believe that tumor biological behavior should be added to the evaluation criteria for downstaging efficacy.Hence,this study aimed to explore the feasibility of Hangzhou criteria(HC),which introduced tumor grading and alpha-fetoprotein in addition to tumor size and number,as an endpoint of downstaging.Methods:We performed a multicenter and retrospective study of 206 patients accepted locoregional therapy(LRT)as downstaging/bridge treatment prior to LT in three centers of China.Results:Recipients were divided into four groups:failed downstaging to the HC(group A,n=46),successful downstaging to the HC(group B,n=30),remained within the HC all the time(group C,n=113),and tumor progressed(group D,n=17).The 3-year HCC recurrence probabilities of groups B and C were not significantly different(10.3%vs.11.6%,P=0.87).The HCC recurrent rate was significantly higher in group A(52.3%)compared with that in group B/C(P<0.05).Seven patients(7/76,9.2%)whose tumor exceeded the the HC were successfully downstaged to the MC,and 39.5%(30/76)to the the HC.In group B,23 patients remained beyond the MC and their survivals were as well as those of patients within the MC.Conclusions:Compared to the MC,HC downstaging criteria can give more HCC patients access to LT and furthermore,the outcome of these patients is the same as those matching MC downstaging criteria.Hangzhou downstaging criteria therefore is applicable in clinical practice. | Qi-Fan Zhan Sun-Bin Ling Yi-Nan Deng Qiao-Nan Shan Qian-Wei Ye Sheng-Jun Xu Guang-Jiang Jiang Di Lu Xu-Yong Wei Li Zhuang Wu Zhang Tian Shen Bei-Ni Cen Hai-Yang Xie Ji-Min Liu Jian Wu Shu-Sen Zheng Yang Yang Xiao Xu | 2020 | Hepatobiliary & Pancreatic Diseases International2020，19，4： | 11 |
| 17 | Two clinically relevant pressures of carbon dioxide pneumoperitoneum cause hepatic injury in a rabbit model显示文摘AIM:To observe the hepatic injury induced by carbon dioxide pneumoperitoneum(CDP) in rabbits,compare the eects olow-and high-pressure pneumoperitoneum,and to determine the degree o hepatic injury induced by these two clinically relevant CDP pressures.METHODS:Thirty healthy male New Zealand rabbits weighing 3.0 to 3.5 kg were randomly divided into three groups(n = 10 for each group) and subjected to the ollowing to CDP pressures:no gas control,10 mmHg,or 15 mmHg.Histological changes in liver tissues were observed with hematoxylin and eosin staining and transmission electron microscopy.Liver unction was evaluated using an automatic biochemical analyzer.Adenine nucleotide translocator(ANT) activity in liver tissue was detected with the atractyloside-inhibitor stop technique.Bax and Bcl-2 expression levels were detected bywestern blotting.RESULTS:Liver Functions in the 10 mmHg and 15 mmHg experimental groups were significantly disturbed compared with the control group.After CDP,the levels or alanine transaminase and aspartate transaminase were 77.3 ± 14.5 IU/L and 60.1 ± 11.4 IU/L,respectively,in the 10 mmHg experimental group and 165.1 ± 19.4 IU/L and 103.8 ± 12.3 IU/L,respectively,in the 15 mmHg experimental group,which were all higher than those of the control group(p < 0.05).There was no difference in pre-albumin concentration between the 10 mmHg experimental group and the control group,but the prealbumin level of the 15 mmHg experimental group was significantly lower than that of the control group(p < 0.05).No significant differences were observed in the levels of total bilirubin or albumin among the three groups.After 30 and 60 min of CDP,pH was reduced(p < 0.05) and fa CO2 was elevated(p < 0.05) in the 10 mmHg group compared with controls,and these changes were more pronounced in the 15 mmHg group.Hematoxylin and eosin staining showed no significant change in liver morphology,except for mild hyperemia in the two experimental groups.Transmission electron microscopy showed mild mitochondrial swelling in hepatocytes of the 10 mmHg group,and this was more pronounced in the 15 mmHg group.No significant difference in ANT levels was found between the control and 10 mmHg groups.However,ANT concentration was significantly lower in the 15 mmHg group compared with the control group.The expression of hepatic Bax was significantly increased in the two experimental groups compared with the controls,but there were no differences in Bcl-2 levels among the three groups.Twelve hours after CDP induction,the expression of hepatic Bax was more significant in the 15 mmHg group than in the 10 mmHg group.CONCLUSION:A CDP pressure of 15 mmHg caused more substantial hepatic injury,such as increased levels of acidosis,mitochondrial damage,and apoptosis;therefore,10 mmHg CDP is preferable for laparoscopic operations. | Jun Li Ying-Hai Liu Zhan-Yong Ye He-Nian Liu Shan Ou Fu-Zhou Tian | 2011 | World Journal of Gastroenterology2011，17，31： | 10 |
| 18 | Biodiversity-based development and evolution:the emerging research systems in model and non-model organisms显示文摘Evolutionary developmental biology,or Evo-Devo for short,has become an established field that,broadly speaking,seeks to understand how changes in development drive major transitions and innovation in organismal evolution.It does so via integrating the principles and methods of many subdisciplines of biology.Although we have gained unprecedented knowledge from the studies on model organisms in the past decades,many fundamental and crucially essential processes remain a mystery.Considering the tremendous biodiversity of our planet,the current model organisms seem insufficient for us to understand the evolutionary and physiological processes of life and its adaptation to exterior environments.The currently increasing genomic data and the recently available gene-editing tools make it possible to extend our studies to non-model organisms.In this review,we review the recent work on the regulatory signaling of developmental and regeneration processes,environmental adaptation,and evolutionary mechanisms using both the existing model animals such as zebrafish and Drosophila,and the emerging nonstandard model organisms including amphioxus,ascidian,ciliates,single-celled phytoplankton,and marine nematode.In addition,the challenging questions and new directions in these systems are outlined as well. | Long Zhao Feng Gao Shan Gao Yujun Liang Hongan Long Zhiyi Lv Ying Su Naihao Ye Liusuo Zhang Chengtian Zhao Xiaoyu Wang Weibo Song Shicui Zhang Bo Dong | 2021 | Science China(Life Sciences)2021，64，8： | 9 |
| 19 | Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes显示文摘Objective Poisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants is inefficient, fallible, and dependent upon experts. In this study, we tested the performance of DNA barcodes for identification of the most threatening poisonous plants in China. Methods Seventy-four accessions of 27 toxic plant species in 22 genera and 17 families were sampled and three DNA barcodes(matK, rbcL, and ITS) were amplified, sequenced and tested. Three methods, Blast, pairwise global alignment(PWG) distance, and Tree-Building were tested for discrimination power. Results The primer universality of all the three markers was high. Except in the case of ITS for Hemerocallis minor, the three barcodes were successfully generated from all the selected species. Among the three methods applied, Blast showed the lowest discrimination rate, whereas PWG Distance and Tree-Building methods were equally effective. The ITS barcode showed highest discrimination rates using the PWG Distance and Tree-Building methods. When the barcodes were combined, discrimination rates were increased for the Blast method. Conclusion DNA barcoding technique provides us a fast tool for clinical identification of poisonous plants in China. We suggest matK, rbcL, ITS used in combination as DNA barcodes for authentication of poisonous plants. | XIE Lei WANG Ying Wei GUAN Shan Yue XIE Li Jing LONG Xin SUN Cheng Ye | 2014 | Biomedical and Environmental Sciences2014，27，10： | 9 |
| 20 | Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation显示文摘Induction and secretion of acid phosphatases(APases) is thought to be an adaptive mechanism that helps plants survive and grow under phosphate(Pi) deprivation. In Arabidopsis, there are 29 purple acid phosphatase(AtPAP)genes. To systematically investigate the roles of different AtPAPs, we first identified knockout or knock‐down T‐DNA lines for all 29 AtPAP genes. Using these atpap mutants combined with in‐gel and quantitative APase enzyme assays,we demonstrated that AtPAP12 and AtPAP26 are two major intracellular and secreted APases in Arabidopsis while AtPAP10is mainly a secreted APase. On Pi‐deficient(P) medium or Pmedium supplemented with the organophosphates ADP and fructose‐6‐phosphate(Fru‐6‐P), growth of atpap10 was significantly reduced whereas growth of atpap12 was only moderately reduced, and growth of atpap26 was nearly equal to that of the wild type(WT). Overexpression of the AtPAP12 or AtPAP26 gene, however, caused plants to grow better on Por P medium supplemented with ADP or Fru‐6‐P. Interestingly, Pi levels are essentially the same for the WT and overexpressing lines, although these two types of plants have significantly different growth phenotypes. These results suggest that the APases may have other roles besides enhancing internal Pi recycling or releasing Pi from external organophosphates for plant uptake. | Liangsheng Wang Shan Lu Ye Zhang Zheng Li Xiaoqiu Du Dong Liu | 2014 | Journal of Integrative Plant Biology2014，56，3： | 9 |
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