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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Rice functional genomics:decades'efforts and roads ahead显示文摘Rice(Oryza sativa L.)is one of the most important crops in the world.Since the completion of rice reference genome sequences,tremendous progress has been achieved in understanding the molecular mechanisms on various rice traits and dissecting the underlying regulatory networks.In this review,we summarize the research progress of rice biology over past decades,including omics,genome-wide association study,phytohormone action,nutrient use,biotic and abiotic responses,photoperiodic flowering,and reproductive development(fertility and sterility).For the roads ahead,cutting-edge technologies such as new genomics methods,high-throughput phenotyping platforms,precise genome-editing tools,environmental microbiome optimization,and synthetic methods will further extend our understanding of unsolved molecular biology questions in rice,and facilitate integrations of the knowledge for agricultural applications. | Rongzhi Chen Yiwen Deng Yanglin Ding Jingxin Guo Jie Qiu Bing Wang Changsheng Wang Yongyao Xie Zhihua Zhang Jiaxin Chen Letian Chen Chengcai Chu Guangcun He Zuhua He Xuehui Huang Yongzhong Xing Shuhua Yang Daoxin Xie Yaoguang Liu Jiayang Li | 2022 | Science China(Life Sciences)2022，65，1： | 12 |
| 3 | Multi-step formation, evolution, and functionalization of new cytoplasmic male sterility genes in the plant mitochondrial genomes显示文摘新基因起源是授与 phenotypic 变化和生物差异的 genomic 革新的主要来源。在植物的新 mitochondrial 基因的产生可以引起细胞质的男绝育(厘米) ,它能支持 outcrossing 和增加健康。然而, mitochondrial 基因怎么在结构和功能发源并且演变,仍然保持不清楚。米饭厘米的野未成功的类型被 mitochondrial 基因 WA352c (以前命名的 WA352 ) 授与并且广泛地在混合米饭繁殖被利用了。这里,我们重建由 11 mitochondrial genomic recombinant 的鉴定和分析的 WA352c 的进化轨道在野、栽培的米饭与 WA352c 有关组织。我们推断这些结构在野米饭 Oryza rufipogon,结合的 substoichiometric 变和顺序变化的 mitochondrial 染色体在保存 mitochondrial 序列之中通过多重重新整理产生了。我们鉴别二表示了,但是在这些结构之中的 nonfunctional protogenes,和他们能发展进功能的厘米基因经由的表演定序能减轻蛋白质的自我禁止的潜力的变化。这些顺序变化将资助蛋白质与编码原子核的 mitochondrial 蛋白质 COX11 交往的能力,在花药 tapetum 和男绝育导致早熟的规划房间死亡。而且,我们证明把 COX11 相互作用领域编码为这些 WA352c 相关的基因的序列经历了在进化期间净化选择。我们经由在结构,顺序,拷贝数字,和功能包含渐渐的变化的 multi-recombination/protogene formation/functionalization 机制为新厘米基因的形成和进化建议一个模型。 | Huiwu Tang Xingmei Zheng Chuliang Li Xianrong Xie Yuanling Chen Letian Chen Xiucai Zhao Huiqi Zheng Jiajian Zhou Shan Ye Jingxin Guo Yao-Guang Liu | 2017 | Cell Research2017，27，1： | 11 |
| 4 | The genetic and molecular basis of cytoplasmic male sterility and fertility restoration in rice显示文摘Cytoplasmic male sterility (CMS) is a maternally inherited characteristic found in many (>150) plant species. CMS/restoration systems are useful tools for hybrid seed production, and are ideal models for study of the interactions between nuclear and mitochondrial genomes. CMS/restoration systems in rice have been widely used for hybrid seed production, greatly contributing to the food supply. This article reviews the progress of the studies on the genetic and molecular basis of cytoplasmic male sterility and fertility restoration in rice. | GUO JingXin LIU YaoGuang | 2009 | Chinese Science Bulletin2009，54，14： | 8 |
| 5 | Detection of a new mutation (1467-A) for the pedigree withmucopolysaccharidosis type Ⅱ from a Chinese family显示文摘Mucopolysaccharidosis type II is of high ge-netic heterogeneity. PCR-DNA sequencing was used to study the mutation hot spots in the IDS gene of a Chinese MPS II pedigree. A new mutation (1467-A) not yet reported world-wide was detected. This mutation located at 448th codon in the coding region of exon 9 deletes one “A” at the end of 1467 bp (cDNA). The frame-shift mutation makes the peptide chain shorten from amino acids 550 to 459, probably altering the configuration of IDS enzyme protein remarkably and lowering the activation of IDS greatly. Therefore it is sup-posed to be the direct cause of the patient with MPS II and to be a necessary premise for prenatal gene diagnosis. | GUO Yibin PAN Jingxin DU Chuanshu | 2005 | Chinese Science Bulletin2005，50，21： | 6 |
| 6 | Fouling corrosion in aluminum heat exchangers显示文摘Fouling deposits on aluminum heat exchanger reduce the heat transfer efficiency and cause corrosion to the apparatus.This study focuses on the corrosive behavior of aluminum coupons covered with a layer of artificial fouling in a humid atmosphere by their weight loss,Tafel plots,electrochemical impedance spectroscopy(EIS),and scanning electron microscope(SEM)observations.The results reveal that chloride is one of the major elements found in the fouling which damages the passive film and initiates corrosion.The galvanic corrosion between the metal and the adjacent carbon particles accelerates the corrosive process.Furthermore,the black carbon favors the moisture uptake,and gives the dissolved oxygen greater chance to migrate through the fouling layer and form a continuous diffusive path.The corrosion rate decreasing over time is conformed to electrochemistry measurements and can be verified by Faraday’s law.The EIS results indicate that the mechanism of corrosion can be interpreted by the pitting corrosion evolution mechanism,and that pitting was observed on the coupons by SEM after corrosive exposure. | Su Jingxin Ma Minyu Wang Tianjing Guo Xiaomei Hou Liguo Wang Zhiping | 2015 | Chinese Journal of Aeronautics2015，28，3： | 5 |
| 7 | Fraction Arithmetic Operations Performed by P Systems显示文摘 | GUO Ping ZHANG Hong CHEN Haizhu CHEN Jingxin | 2013 | Chinese Journal of Electronics2013，22，4： | 4 |
| 8 | Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants显示文摘Dear Editor,CRISPR(clustered regularly interspaced short palindromic repeats)/Cas genome editing is a powerful tool for introducing specific mutations in organisms including plants.The system is composed of a nuclease such as Cas9 or Cas12a and an engineered single-guide RNA(sgRNA)incorporating a target sequence(Li et al.,2019).A Cas9/sgRNA complex recognizes its target site in the genome,resulting in a mutation at that site. | Yu Hao Wubei Zong Dongchang Zeng Jingluan Han Shuifu Chen Jianian Tang Zhe Zhao Xiaojuan Li Kun Ma Xianrong Xie Qinlong Zhu Yuanling Chen Xiucai Zhao Jingxin Guo Yao-Guang Liu | 2020 | Science China(Life Sciences)2020，63，6： | 4 |
| 9 | Hd1,Ghd7,and DTH8 synergistically determine the rice heading date and yield-related agronomic traits显示文摘Heading date determines the seasonal and regional adaptation of rice(Oryza sativa L.)varieties and is mainly controlled by photoperiod sensitivity(PS).The core heading date genes Hd1,Ghd7,DTH8,and PRR37 act synergistically in regulating the PS.In this study,we systematically analyze the heading date,PS,and agronomic traits of eight homozygous lines with various combinations of Hd1,Ghd7,and DTH8 alleles in the prr37 background under long-day(LD)and short-day(SD)conditions,respectively.We find that Hd1 alone promotes heading,regardless of the day length.However,under LDs,Hd1 suppresses flowering,in coordination with functional Ghd7 or with Ghd7 and DTH8.These loci cooperate to negatively regulate the Ehd1-Hd3 a/RFT1 pathway and delay heading.Under SDs,Hd1 competes with various heading suppressors to promote heading.Therefore,the dual function of Hd1 is vital for PS.The lines carrying Hd1 alone show reduced plant height with fewer primary and secondary branches in panicles.Lines carrying Ghd7 and DTH8(with hd1)show delayed heading and improve agronomic traits.Overall,our results reveal the regulation of rice PS flowering by the core heading date genes and their effects on agronomic traits,providing valuable information for the selection of rice varieties for adaptation to different light and temperature conditions. | Kangli Sun Minghui Huang Wubei Zong Dongdong Xiao Chen Lei Yanqiu Luo Yingang Song Shengting Li Yu Hao Wanni Luo Bingqun Xu Xiaotong Guo Guangliang Wei Letian Chen Yao-Guang Liu Jingxin Guo | 2022 | Journal of Genetics and Genomics2022，49，5： | 4 |
| 10 | Epithelial-mesenchymal transition status of circulating tumor cells in breast cancer and its clinical relevance显示文摘Objective:Circulating tumor cells(CTCs)play a critical role in cancer metastasis,but their prevalence and significance remain unclear.This study attempted to track the epithelial-mesenchymal transition(EMT)status of CTCs in breast cancer patients and investigate their clinical relevance.Methods:In this study,the established negFACS-IF:E/M platform was applied to isolate rare CTCs and characterize their EMT status in breast cancer.A total of 89 breast cancer patients were recruited,including stage 0–III(n=60)and late stage(n=29)cases.Results:Using the negFACS-IF:E/M platform,it was found that in human epidermal growth factor receptor 2(HER2)+patients,mesenchymal CTCs usually exhibited a high percentage of HER2+cells.Stage IV breast cancer patients had considerably more CTCs than stage 0–III patients.Among stage 0–III breast cancers,the HER2 subtype included a significantly higher percentage of mesenchymal and biphenotypic(epithelial and mesenchymal)CTCs than the luminal A or B subtypes.Among stage IV patients,CTCs were predominantly epithelial in cases with local recurrence and were more mesenchymal in cases with distant metastasis.By applying a support vector machine(SVM)algorithm,the EMT status of CTCs could distinguish between breast cancer cases with metastasis/local recurrence and those without recurrence.Conclusions:The negFACS-IF:E/M platform provides a flexible and generally acceptable method for the highly sensitive and specific detection of CTCs and their EMT traits in breast cancer.This study demonstrated that the EMT status of CTCs had high clinical relevance in breast cancer,especially in predicting the distant metastasis or local recurrence of breast cancer. | Jiaojiao Zhou Xuan Zhu Shijie Wu Jingxin Guo Kun Zhang Chunjing Xu Huihui Chen Yuxi Jin Yuting Sun Shu Zheng Yiding Chen | 2020 | Cancer Biology & Medicine2020，17，1： | 3 |
| 11 | Transarterial chemoembolization in combination with programmed death-1/programmed cell death-ligand 1 immunotherapy for hepatocellular显示文摘Hepatocellular carcinoma(HCC)is one of the most common malignancies worldwide.With the development of systemic therapies,several studies are currently underway,especially those related to the use of programmed death-1/programmed cell death-ligand 1(PD-1/PD-L1)immunotherapy.Moreover,studies on transarterial che-moembolization(TACE)and PD-1/PD-L1 immunotherapy have demonstrated some interesting outcomes.This article reviewed the current clinical evidence on the combination of TACE and PD-1/PD-L1 immunotherapy.Overall,our review summarized that a favorable survival time could be achieved using this combination in most patients.However,complications such as hyperprogression should be taken seriously,and the underlying mechanisms need to be explored. | Jingxin Yan Manjun Deng Shunyu Kong Ting Li Zhenwu Lei Lushun Zhang Yutong Zhuang Xin He Huanwei Wang Haining Fan Yingxing Guo | 2022 | iLIVER2022，1，4： | 2 |
| 12 | Long non-coding RNAs play an important role in regulating photoperiod-and temperature-sensitive male sterility in rice显示文摘Rice(Oryza sativa L.)is a major food crop for more than half of the world’s population.Therefore,increasing rice production is of great importance.The successful development of hybrid rice in the 1970s was an important achievement for increasing yield potential,since hybrid rice varieties produce higher yields than inbred varieties(Cheng et al.,2007).In plants,male sterility refers to the inability of producing dehiscent anthers and functional pollen(Chen and Liu,2014). | Jingxin Guo Yao-Guang Liu | 2017 | Science China(Life Sciences)2017，60，4： | 2 |
| 13 | Efficacy and complications of transarterial chemoembolization alone or in combination with different protocols for hepatocellular carcinoma: A Bayesian network meta-analysis of randomized controlled trials显示文摘Background and aims:Transarterial chemoembolization(TACE)is the standard treatment for most intermediate-toadvanced stage hepatocellular carcinoma.With the ongoing development of interventional therapies,TACE in combination with different protocols is being explored.A network meta-analysis to collect recent evidence in this field is required.Methods:We searched PubMed,Web of Science,Cochrane Central Register of Controlled Trials,Embase,Chinese National Knowledge Infrastructure database,Cochrane Library,and Wanfang database from their inception to May 2022 for randomized controlled trials(RCTs)that reported TACE in combination with different protocols for hepatocellular carcinoma.Results:In total,67 RCTs assessing eight combination treatments were included.The quality of primary outcomes was from moderate to low.The Bayesian network meta-analysis confirmed that TACE combined with iodine-125 seed implantation had the highest probability of 1-year survival rate and TACE combined with sorafenib had the highest probability of 2-year survival rate.TACE alone had the highest probability of incidence of fever,TACE combined with sorafenib had the highest probability of incidence of pain and TACE combined with percutaneous ethanol injection had the highest probability of incidence of nausea and vomiting.Conclusion:This Bayesian network meta-analysis found that TACE combined with iodine-125 seed and TACE combined with sorafenib may benefit patients in terms of 1-year survival and 2-year survival,respectively.To confirm this conclusion,high-quality and well-designed RCTs with a larger sample size are needed. | Jingxin Yan Manjun Deng Ting Li Changhao Dong Minglan Wang Shunyu Kong Yingxing Guo Haining Fan | 2023 | iLIVER2023，2，2： | 1 |
| 14 | REE bound DNA in natural plant显示文摘 | Yuqi Wang Ping Jiang Fanqing Guo Zhiyong Zhang Jingxin Sun Lei Xu Guoyin Cao | 1999 | Science in China Series B: Chemistry1999，，4： | 1 |
| 15 | REE bound polysaccharides in leaves of Dicranopteris dichotoma by MAA显示文摘 | Yuqi Wang Jingxin Sun Hongmin Chen Fanqing Guo Lei Xu Guoyin Cao | 1998 | Science in China Series B: Chemistry1998，，3： | 1 |
| 16 | LIFU-responsive nanomedicine enables acoustic droplet vaporization-induced apoptosis of macrophages for stabilizing vulnerable atherosclerotic plaques显示文摘Due to the high risk of tearing and rupture,vulnerable atherosclerotic plaques would induce serious cardiovascular and cerebrovascular diseases.Despite the available clinical methods can evaluate the vulnerability of plaques and specifically treat vulnerable plaques before a cardiovascular event,but the efficiency is still low and undesirable.Herein,we rationally design and engineer the low-intensity focused ultrasound(LIFU)-responsive FPD@CD nanomedicine for the highly efficient treatment of vulnerable plaques by facilely loading phase transition agent perfluorohexane(PFH)into biocompatible PLGA-PEG-PLGA nanoparticles(PPP NPs)and then attaching dextran sulphate(DS)onto the surface of PPP NPs for targeting delivery.DS,as a typical macrophages-targeted molecule,can achieve the precise vaporization of NPs and subsequently controllable apoptosis of RAW 264.7 macrophages as induced by acoustic droplet vaporization(ADV)effect.In addition,the introduction of DiR and Fe3O4 endows nanomedicine with near-infrared fluorescence(NIRF)and magnetic resonance(MR)imaging capabilities.The engineered FPD@CD nanomedicine that uses macrophages as therapeutic targets achieve the conspicuous therapeutic effect of shrinking vulnerable plaques based on in vivo and in vitro evaluation outcomes.A reduction of 49.4%of vascular stenosis degree in gross pathology specimens were achieved throughout the treatment period.This specific,efficient and biosafe treatment modality potentiates the biomedical application in patients with cardiovascular and cerebrovascular diseases based on the relief of the plaque rupture concerns. | Jingxin Hou Jun Zhou Meiqi Chang Guangcheng Bao Jie Xu Man Ye Yixin Zhong Shuling Liu Junrui Wang Wei Zhang Haitao Ran Zhigang Wang Yu Chen Dajing Guo | 2022 | Bioactive Materials2022，7，10： | 1 |
| 17 | Synthesis and characterization of an acrylate-copolymer-based antistatic agent composed of a single-ion conductive polymer electrolyte显示文摘 | Guo Dongyang Wang Jiliang Lei Jingxin | 2011 | Journal Applied Polymer Science2011，119，12： | 1 |
| 18 | Econometrical investigation on infrastructure investment and economic development in China: A case study using vector autoregression approach显示文摘 | Guo Jianhua Guo Jianping Xia Jingxin | 2011 | KSCE Journal of Civil Engineering2011，15，3： | 1 |
| 19 | Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferation显示文摘Plant height is an important agronomic trait for lodging resistance and yield.Here,we report a new plantheight-related gene,OsUBR7 in rice(Oryza sativa L.);knockout of OsUBR7 caused fewer cells in internodes,resulting in a semi-dwarf phenotype.OsUBR7 encodes a putative E3 ligase containing a plant homeodomain finger and a ubiquitin protein ligase E3 component N-recognin 7(UBR7)domain.OsUBR7 interacts with histones and monoubiquitinates H2B(H2Bub1)at lysine148 in coordination with the E2 conjugase OsUBC18.OsUBR7 mediates H2Bub1 at a number of chromatin loci for the normal expression of target genes,including cell-cycle-related and pleiotropic genes,consistent with the observation that cell-cycle progression was suppressed in the osubr7 mutant owing to reductions in H2Bub1 and expression levels at these loci.The genetic divergence of OsUBR7 alleles among japonica and indica cultivars affects their transcriptional activity,and these alleles may have undergone selection during rice domestication.Overall,our results reveal a novel mechanism that mediates H2Bub1 in plants,and UBR7 orthologs could be utilized as an untapped epigenetic resource for crop improvement. | Yangyi Zheng Sensen Zhang Yanqiu Luo Fuquan Li Jiantao Tan Bin Wang Zhe Zhao Huifang Lin Tingting Zhang Jianhong Liu Xupeng Liu Jingxin Guo Xianrong Xie Letian Chen Yao-Guang Liu Zhizhan Chu | 2022 | Plant Communications2022，3，6： | 0 |
| 20 | An integrative drug repositioning framework discovered a potential therapeutic agent targeting COVID-19显示文摘The global spread of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)requires an urgent need to find effective therapeutics for the treatment of coronavirus disease 2019(COVID-19).In this study,we developed an integrative drug repositioning framework,which fully takes advantage of machine learning and statistical analysis approaches to systematically integrate and mine large-scale knowledge graph,literature and transcriptome data to discover the potential drug candidates against SARS-CoV-2.Our in silico screening followed by wet-lab validation indicated that a poly-ADP-ribose polymerase 1(PARP1)inhibitor,CVL218,currently in Phase I clinical trial,may be repurposed to treat COVID-19.Our in vitro assays revealed that CVL218 can exhibit effective inhibitory activity against SARS-CoV-2 replication without obvious cytopathic effect.In addition,we showed that CVL218 can interact with the nucleocapsid(N)protein of SARS-CoV-2 and is able to suppress the LPS-induced production of several inflammatory cytokines that are highly relevant to the prevention of immunopathology induced by SARS-CoV-2 infection. | Yiyue Ge Tingzhong Tian Suling Huang Fangping Wan Jingxin Li Shuya Li Xiaoting Wang Hui Yang Lixiang Hong Nian Wu Enming Yuan Yunan Luo Lili Cheng Chengliang Hu Yipin Lei Hantao Shu Xiaolong Feng Ziyuan Jiang Yunfu Wu Ying Chi Xiling Guo Lunbiao Cui Liang Xiao Zeng Li Chunhao Yang Zehong Miao Ligong Chen Haitao Li Hainian Zeng Dan Zhao Fengcai Zhu Xiaokun Shen Jianyang Zeng | 2021 | Signal Transduction and Targeted Therapy2021，6，5： | 0 |
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