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| 1 | Single-cell RNA sequencing in breast cancer: Understanding tumor heterogeneity and paving roads to individualized therapy显示文摘Single-cell RNA sequencing(scRNA-seq)is a novel technology that allows transcriptomic analyses of individual cells.During the past decade,scRNA-seq sensitivity,accuracy,and efficiency have improved due to innovations including more sensitive,automated,and cost-effective single-cell isolation methods with higher throughput as well as ongoing technological development of scRNA-seq protocols.Among the variety of current approaches with distinct features,researchers can choose the most suitable method to carry out their research.By profiling single cells in a complex population mix,scRNA-seq presents great advantages over traditional sequencing methods in dissecting heterogeneity in cell populations hidden in bulk analysis and exploring rare cell types associated with tumorigenesis and metastasis.scRNA-seq studies in recent years in the field of breast cancer research have clustered breast cancer cell populations with different molecular subtypes to identify distinct populations that may correlate with poor prognosis and drug resistance.The technology has also been used to explain tumor microenvironment heterogeneity by identifying distinct immune cell subsets that may be associated with immunosurveillance and are potential immunotherapy targets.Moreover,scRNA-seq has diverse applications in breast cancer research besides exploring heterogeneity,including the analysis of cell-cell communications,regulatory single-cell states,immune cell distributions,and more.scRNA-seq is also a promising tool that can facilitate individualized therapy due to its ability to define cell subsets with potential treatment targets.Although scRNA-seq studies of therapeutic selection in breast cancer are currently limited,the application of this technology in this field is prospective.Joint efforts and original ideas are needed to better implement scRNA-seq technologies in breast cancer research to pave the way for individualized treatment management.This review provides a brief introduction on the currently available scRNA-seq approaches along with their corresponding strengths and weaknesses and may act as a reference for the selection of suitable methods for research.We also discuss the current applications of scRNA-seq in breast cancer research for tumor heterogeneity analysis,individualized therapy,and the other research directions mentioned above by reviewing corresponding published studies.Finally,we discuss the limitations of current scRNA-seq technologies and technical problems that remain to be overcome. | Shuning Ding Xiaosong Chen Kunwei Shen | 2020 | Cancer Communications2020，40，8： | 12 |
| 2 | Antibiotic growth promoter olaquindox increases pathogen susceptibility in fish by inducing gut microbiota dysbiosis显示文摘Low dose antibiotics have been used as growth promoters in livestock and fish. The use of antibiotics has been associated with reduced pathogen infections in livestock. In contrast, antibiotic growth promoter has been suspected of leading to disease outbreaks in aquaculture. However, this phenomenon is circumstantial and has not been confirmed in experimental conditions. In this study,we showed that antibiotic olaquindox increased the susceptibility of zebrafish to A. hydrophila infection. Olaquindox led to profound alterations in the intestinal microbiota of zebrafish, with a drastic bloom of Enterobacter and diminishing of Cetobacterium. Moreover, the innate immune responses of zebrafish were compromised by olaquindox(P<0.05). Transfer of microbiota to GF zebrafish indicated that while the immuo-suppression effect of olaquindox is a combined effect mediated by both OLA-altered micro biota and direct action of the antibiotic(P<0.05), the increased pathogen susceptibility was driven by the OLA-altered microbiota and was not dependent on direct antibiotic effect. Taken together, these data indicate that low level of OLA induced gut microbiota dysbiosis in zebrafish, which led to increased pathogen susceptibility. | suxu he quanmin wang shuning li chao ran xiaoze guo zhen zhang zhigang zhou | 2017 | Science China(Life Sciences)2017，60，11： | 10 |
| 3 | SKIP Confers Osmotic Tolerance during Salt Stress by Controlling Alternative Gene Splicing in Arabidopsis显示文摘译解位于下面的机制种回答到不能生活的应力为改进植物压力抵抗是关键的。大部分在 transcriptional 水平响应盐应力对基因表示的规定被知道;然而,很少在 posttranscriptional 水平对这个过程被知道。最近,我们证明那个翻斗车是与钟基因 pre-mRNAs 交往并且为调整他们拼接的选择和 mRNA 成熟是必要的 spliceosome 的一个部件。在这研究,我们发现 skip-1 植物对盐和渗透的压力过分敏感,并且那个翻斗车为拼接的选择和几咸忍耐的基因的 mRNA 成熟被要求,包括 NHX1, CBL1, P5CS1, RCI2A,和 PAT10。染色体宽的分析表明那个翻斗车调停在咸压力的条件下面许多基因拼接的选择,和在 skip-1 的其他的拼接事件的那个大多数包含 intron 保留和罐头 generatea 在抄录 mRNA 的早熟的结束 codon。翻斗车也控制由 modulating 的其他的拼接 5 和 3 的识别或劈开拼接在咸压力的条件下面的施主和领受人地点。因此,这研究探讨的基本问题拼接的 mRNA 在植物的机械在 posttranscriptional 水平贡献咸压力的回答,并且提供在其他的拼接和盐忍耐之间的一个连接。 | Jinlin Feng dingjing Li Zhaoxu Gao Yaru Lu Junya Yu Qian Zheng Shuning Yan Wenjiao Zhang Hang He Ligeng Ma Zhengge Zhu | 2015 | Molecular Plant2015，8，7： | 10 |
| 4 | A model for parameter estimation of multistage centrifugal compressor and compressor performance analysis using genetic algorithm显示文摘A model for performance prediction of multistage centrifugal compressor is proposed.The model allows the users to predict the compressor performance,e.g.pressure ratio,efficiency and losses using the compressor geometric information and speed by a stage stacking calculation based on the characteristics of each stage.To develop the compressor elemental stage characteristics,the compressor losses,such as incidence losses and friction losses,are mathematically modeled.For a composite systems,for instance a gas turbine power plant,the performance of the multistage centrifugal compressor can be evaluated.Since some important parameters of the compressor model,e.g.,the slip factor,shock loss coefficient and reference diameter D1,are hard to be determined by empirical laws,a genetic algorithm(GA) is used to solve the parameter estimation problem of the proposed model,and in turn the compressor performance analysis and parameters study are performed.The surge line for the multistage centrifugal compressor can also be determined from the simulation results.Furthermore,the model presented here provides a valuable tool for evaluating the multistage centrifugal compressor performance as a function of various operation parameters. | CHU Fei WANG FuLi WANG XiaoGang ZHANG ShuNing | 2012 | Science China(Technological Sciences)2012，55，11： | 8 |
| 5 | State of the Art and Prospects in Metal-Organic Framework-Derived Microwave Absorption Materials显示文摘Microwave has been widely used in many fields,including communication,medical treatment and military industry;however,the corresponding generated radiations have been novel hazardous sources of pollution threating human’s daily life.Therefore,designing high-performance microwave absorption materials(MAMs)has become an indispensable requirement.Recently,metal-organic frameworks(MOFs)have been considered as one of the most ideal precursor candidates of MAMs because of their tunable structure,high porosity and large specific surface area.Usually,MOF-derived MAMs exhibit excellent electrical conductivity,good magnetism and sufficient defects and interfaces,providing obvious merits in both impedance matching and microwave loss.In this review,the recent research progresses on MOF-derived MAMs were profoundly reviewed,including the categories of MOFs and MOF composites precursors,design principles,preparation methods and the relationship between mechanisms of microwave absorption and microstructures of MAMs.Finally,the current challenges and prospects for future opportunities of MOF-derived MAMs are also discussed. | Shuning Ren Haojie Yu Li Wang Zhikun Huang Tengfei Lin Yudi Huang Jian Yang Yichuan Hong Jinyi Liu | 2022 | Nano-Micro Letters2022，14，4： | 6 |
| 6 | An Adaptive Weighted Differential Game Guidance Law显示文摘For intercepting modern high maneuverable targets, a novel adaptive weighted differential game guidance law based on the game theory of mixed strategy is proposed, combining two guidance laws which are derived from the perfect and imperfect information pattern, respectively. The weights vary according to the estimated error of the target's acceleration, the guidance law is generated by directly using the estimation of target's acceleration when the estimated error is small, and a differential game guidance law with adaptive penalty coefficient is implemented when the estimated error is large. The adaptive penalty coefficients are not constants and they can be adjusted with current target maneuverability. The superior homing performance of the new guidance law is verified by computer simulations. | ZHANG Ping FANG Yangwang ZHANG Fengming XIAO Bingsong HU Shiguo ZONG Shuning | 2012 | Chinese Journal of Aeronautics2012，25，5： | 5 |
| 7 | Review of lithium-ion battery state of charge estimation显示文摘The technology deployed for lithium-ion battery state of charge(SOC)estimation is an important part of the design of electric vehicle battery management systems.Accurate SOC estimation can forestall excessive charging and discharging of lithium-ion batteries,thereby improving discharge efficiency and extending cycle life.In this study,the key lithium-ion battery SOC estimation technologies are summarized.First,the research status of lithium-ion battery modeling is introduced.Second,the main technologies and difficulties in model parameter identification for lithium-ion batteries are discussed.Third,the development status and advantages and disadvantages of SOC estimation methods are summarized.Finally,the current research problems and prospects for development trends are summarized. | Ning Li Yu Zhang Fuxing He Longhui Zhu Xiaoping Zhang Yong Ma Shuning Wang | 2021 | Global Energy Interconnection2021，4，6： | 5 |
| 8 | Preparation of microbial desulfurization catalysts显示文摘A Rhodococcus sp. lawq, a bacterium isolated from the soil cleaving the C-S bond of dibenzothiophene (DBT) via specific pathway, was investigated for cell growth and for its role in desulfurization. Clearly, the end product, 2-hydroxybiphenyl, inhibited the growth of the strain, the synthesis of the desulfurization enzymes, and the activity of the enzymes. The effects of sulfate on the DBT degradation enzymes were examined in the Rhodococcus sp. lawq growth system with DBT; the sulfate served, concurrently, as the sulfur source. The condition of the resting cells that were used in desulfurization, was also studied. The optimal concentration of the resting cells and the reaction conditions were determined. It was documented that there is no difference between desulfurization activity for resting cells cultured with sulfate as the sole sulfur source and that with the mixture of DBT and sulfate as the sulfur source. | XU Ping MA Cuiqing LI Fuli TONG Mingyou ZENG Yiyong WANG Shuning H.D.Blankespoor | 2002 | Chinese Science Bulletin2002，47，13： | 4 |
| 9 | A triple-RBD-based mucosal vaccine provides broad protectionagainst SARS-CoV-2 variants of concern显示文摘The rapid mutation and spread of SARS-CoV-2 variants urge the development of effective mucosal vaccines to provide broadspectrum protection against the initial infection and thereby curb the transmission potential.Here,we designed a chimeric tripleRBD immunogen,3Ro-NC,harboring one Delta RBD and two Omicron RBDs within a novel protein scaffold.3Ro-NC elicits potent and broad RBD-specific neutralizing immunity against SARS-CoV-2 variants of concern.Notably,intranasal immunization with 3RoNC plus the mucosal adjuvant KFD(3Ro-NC+KFDi.n)elicits coordinated mucosal IgA and higher neutralizing antibody specificity(closer antigenic distance)against the Omicron variant.In Omicron-challenged human ACE2 transgenic mice,3Ro-NC+KFDi.n immunization significantly reduces the tissue pathology in the lung and lowers the viral RNA copy numbers in both the lung(85.7-fold)and the nasal turbinate(13.6-fold).Nasal virologic control is highly correlated with RBD-specific secretory IgA antibodies.Our data show that 3Ro-NC plus KFD is a promising mucosal vaccine candidate for protection against SARS-CoV-2 Omicron infection,pathology and transmission potential. | Jingyi Yang Mei-Qin Liu Lin Liu Xian Li Mengxin Xu Haofeng Lin Shuning Liu Yunqi Hu Bei Li Bowen Liu Min Li Ying Sun Yao-Qing Chen Zheng-Li Shi Huimin Yan | 2022 | Cellular & Molecular Immunology2022，19，11： | 3 |
| 10 | Glycerolipid Profiling of Yellow Sarson Seeds Using Ultra High Performance Liquid Chromatography Coupled to Triple Time-of-Flight Mass Spectrometry显示文摘Yellow sarson(Brassica rapa ssp. trillocularis) is an important rapeseed-mustard species of Brassica rapa due to its high seed oil content. Glycerolipids and fatty acid composition affect seed germination and determine the quality of seed oil. To date, no information is available on the composition of individual glycerolipids in this species. Therefore, in this study the glycerolipid profiling of yellow sarson seeds was performed using ultra high performance liquid chromatography coupled to triple time-of-flight mass spectrometry(UPLC-Triple-TOF-MS).A fast and efficient chromatographic separation of glycerolipids was accomplished based on an UPLCTMBEH C8 column within 22 min.In ESI positive ion mode, TOF-MS scan-information dependent acquisition-product ion scan was carried out to acquire both high resolution MS and MS/MS information from one injection. According to MS/MS spectra, predominant fragmentation patterns of glycerolipids were elucidated in detail. Based on retention time, accurate mass, isotopic distribution, and fragmentation patterns, the composition of 144 glycerolipids and fatty acids were finally identified in yellow sarson seeds, including 77 triacylglycerols, 32 diacylglycerols, 18sulfoquinovosyl-diacylglycerols, 5 monogalactosyl-diaclyglycerols, and 12 digalactosyl-diacylglycerols. Of them, the most abundant glycerolipids in yellow sarson seeds were triacylglycerols, the major storage form of seed oil in plants. In addition, diacylglycerols were found as a minor component of glycerolipids. The lowest amounts of glycerolipids detected in seeds were glycosyl-acylglycerols. The results revealed the composition and relative content of glycerolipids in yellow sarson seeds, which will provide a more comprehensive assessment of the quality of seed oil and also help to select functional cultivars with higher beneficial glycerolipids. This profiling method has the advantages of high throughput, high sensitivity and good accuracy, which will provide a reliable analysis platform for glycerolipids metabolism in plants. | ZHENG Shuning ZHANG Yanguo LIU Guangyang LV Jun LIU Zhongxiao XU Xiaomin LI Lingyun XU Donghui | 2017 | Horticultural Plant Journal2017，3，4： | 2 |
| 11 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 12 | ARIMA and Facebook Prophet Model in Google Stock Price Prediction显示文摘We use the Autoregressive Integrated Moving Average(ARIMA)model and Facebook Prophet model to predict the closing stock price of Google during the COVID-19 pandemic as well as compare the accuracy of these two models’predictions.We first examine the stationary of the dataset and use ARIMA(0,1,1)to make predictions about the stock price during the pandemic,then we train the Prophet model using the stock price before January 1,2021,and predict the stock price after January 1,2021,to present.We also make a comparison of the prediction graphs of the two models.The empirical results show that the ARIMA model has a better performance in predicting Google’s stock price during the pandemic. | Beijia Jin Shuning Gao Zheng Tao | 2022 | Proceedings of Business and Economic Studies2022，5，5： | 2 |
| 13 | Single-cell atlas reveals a distinct immune profile fostered by T cell-B cell crosstalk in triple negative breast cancer显示文摘Background:Characterizing the unique immune microenvironment of each tumor is of great importance for better predicting prognosis and guiding cancer immunotherapy.However,the unique features of the immune microenvironment of triple negative breast cancer(TNBC)compared with other subtypes of breast cancer remain elusive.Therefore,we aimed to depict and compare the immune landscape among TNBC,human epidermal growth factor receptor 2-positive(HER2^(+))breast cancer,and luminal-like breast cancer.Methods:Single-cell RNA sequencing(scRNA-seq)was performed on CD45^(+)immune cells isolated from human normal breast tissues and primary breast tumors of various subtypes.By analyzing the scRNA-seq data,immune cell clusters were identified and their proportions as well as transcriptome features were compared among TNBC,human HER2^(+)breast cancer,and luminal-like breast cancer.Pseudotime and cell-cell communication analyses were also conducted to characterize the immune microenvironment.Results:ScRNA-seq data of 117,958 immune cells were obtained and 31 immune clusters were identified.A unique immunosuppressive microenvironment in TNBC was decoded as compared to that in HER2^(+)or luminal-like breast cancer,which was characterized by higher proportions of regulatory T cells(Tregs)and exhausted CD8+T cells and accompanied by more abundant plasma cells.Tregs and exhausted CD8+T cells in TNBC exhibited increased immunosuppression signature and dysfunctional scores.Pseudotime analyses showed that B cells tended to differentiate to plasma cells in TNBC.Cell-cell communication analyses indicated that these unique features are fostered by the diversified T cell-B cell crosstalk in TNBC.Based on the T cell-B cell crosstalk,a prognostic signaturewas established that could effectively predict the prognosis status for patients with TNBC.Additionally,it was found that TNBC had a higher proportion of cytotoxic natural killer(NK)cells,whereas HER2^(+)or luminal-like breast cancer lost this feature,suggesting thatHER2^(+)or luminal-like breast cancer,but not TNBC,may benefit from NK-based immunotherapy.Conclusions:This study identified a distinct immune feature fostered by T cell-B cell crosstalk in TNBC,which provides better prognostic information and effective therapeutic targets for breast cancer. | Shuning Ding Niu Qiao Qingchen Zhu Yiwei Tong Shengyue Wang Xiaosong Chen Qiang Tian Yichuan Xiao Kunwei Shen | 2023 | Cancer Communications2023，43，6： | 2 |
| 14 | EDGE DETECTION METHOD OF REMOTE SENSING IMAGES BASED ON MATHEMATICAL MORPHOLOGY OF MULTI-STRUCTURE ELEMENTS显示文摘This paper puts forward an effective, specific algorithm for edge detection. Based on multi-structure elements of gray mathematics morphology, in the light of difference between noise and edge shape of RS images, the paper establishes multi-structure elements to detect edge by utilizing the grey form transformation principle. Compared with some classical edge detection operators,such as Sobel Edge Detection Operator, LOG Edge Detection Operator, and Canny Edge Detection Operator, the experiment indicates that this new algorithm possesses very good edge detection ability, which can detect edges more effectively, but its noise-resisting ability is relatively low. Because of the bigger noise of remote sensing image, the authors probe into putting forward other edge detection method based on combination of wavelet directivity checkout technology and small-scale Mathematical Morphology finally. So, position at the edge can be accurately located, the noise can be inhibited to a certain extent and the effect of edge detection is obvious. | LINHui DUPei-jun ZHAOChang-sheng SHUNing | 2004 | Chinese Geographical Science2004，14，3： | 2 |
| 15 | An oral second-generation proteasome inhibitor oprozomib significantly inhibits lung cancer in a p53 independent manner in vitro显示文摘The destruction of proteins via the ubiquitin-proteasome system is amulti-step,complex process involving polyubiquitination of substrate proteins,follow ed by proteolytic degradation by the m acrom olecular 26S proteasome complex.Inhibitors of the proteasome prom ote the accum ulation of proteins that are deleterious to cell survival and are prom ising anticancer agents.Oprozomib(OPZ),an oral second-generation proteasome inhibitor,has been shown to inhibit the grow th of several cancers in preclinical and clinical trials,including m ultiple m yelom a and head and neck cancers,but its effects on lung cancer has not yet been determ ined.In this study,we evaluated the inhibitory effects of OPZ on lung cancer cell lines in vitro.The results showed that OPZ significantly suppressed cell proliferation and strongly induced apoptosis in both tested lung cancer cells independent of p53 expression.OPZ was able to cause obvious caspase 3 and PARP cleavages and stabilize p53 and its transcriptional targets p21,PUMA,and Noxa.Moreover,OPZ was capable of sensitizing lung cancer cells to the conventional chem otherapeutic drug cisplatin.Our study provides preclinical data and sheds light on the potential applications of proteasome inhibitor OPZ in lung cancer treatment. | Hongge Zhu Tianhai Wang Zhou Xin Yiyi Zhan Guoming Gu Xiaoqin Li Xiuli Wang Shune Yang Chunling Liu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，10： | 2 |
| 16 | Classification of Coronary Atherosclerotic Plaques Ex Vivo With T1, T2, and Ultrashort Echo Time CMR显示文摘 | Mihály Károlyi Harald Seifarth Gary Liew Christopher L. Schlett Pál Maurovich-Horvat Paul Stolzmann Guangping Dai Shuning Huang Craig J. Goergen Masataka Nakano Fumiyuki Otsuka Renu Virmani Udo Hoffmann David E. Sosnovik | 2013 | JACC: Cardiovascular Imaging2013，，4： | 1 |
| 17 | General representation for continuous piecewise-linear functions 显示文摘 | WANG Shuning | 2004 | IEEE Trans Circuits Syst2004，51，9： | 1 |
| 18 | Effects of Statin Therapy on Inflammatory Markers in Chronic Heart Failure: A Meta-analysis of Randomized Controlled Trials显示文摘 | Lei Zhang Shuning Zhang Hong Jiang Aijun Sun Yunkai Wang Yunzeng Zou Junbo Ge Haozhu Chen | 2010 | Archives of Medical Research2010，，6： | 1 |
| 19 | Dynamic mechanical and thermal properties of copolymer from o-allylphenol and 4,40-diaminodiphenyl methane-based benzoxazine and bisphenol-A type novolac epoxy resin显示文摘 | Hao Zhanzhan Lv Shufang Song Shuning | 2015 | J Therm Anal Calorim2015，119，： | 1 |
| 20 | From Droplets and Particles to Hierarchical Spatial Organization: Nanotechnology Challenges for Microfluidics显示文摘 | J. Michael Kohler P. Mike Gvnther Anette Funfak Jialan Cao Andrea Knauer Shuning Li Steffen Schneider G. Alexander Gross | 2011 | Journal of Physical Science and Application2011，1，3： | 1 |
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