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| 1 | Overexpression of annexin 1 in pancreatic cancer and its clinical significance显示文摘AIM: To investigate the expression of annexin I in pancreatic cancer and its relationship with the clinicopathologic factors,and to evaluate its potential clinical significance.METHODS: Annexin I expression was analyzed by Western blot and immunohistochemical staining in pancreatic adenocarcinoma and multi-tissue microarrays (MTAs).RESULTS: Western blot analysis showed that annexin I was overexpressed in 84.6% (11/13) pancreatic ductal adenocarcinomas. Immunohistochemistry analysis of pancreatic cancer in MTAs showed that annexin I protein was 71.4%(30/42) positive which was markedly increased compared with that in the tumor matched normal pancreas tissues 18.4%(7/38) (P<0.01). In the meantime, the high expression of annexin 1 was correlated with the poor differentiation of pancreatic adenocarcinoma.CONCLUSION: Annexin 1 overexpression is a frequent biological marker and correlates with the differentiation of pancreatic cancer during tumorigenesis. | Xiao-FengBai Xiao-GuangNi PingZhao Shang-MeiLiu Hui-XinWang BingGuo Lan-PingZhou FangLiu Jin-ShengZhang KunWang Yong-Qiangxie Yong-FuShao Xiao-HangZhao | 2004 | World Journal of Gastroenterology2004，10，10： | 40 |
| 2 | Risk factors for alcoholic liver disease in China显示文摘AIM: To examine the association of daily alcohol intake,types of alcoholic beverage consumed, drinking patterns and obesity with alcoholic liver disease in China.METHODS: By random cluster sampling and a 3-year follow-up study, 1 270 alcohol drinkers were recruited from different occupations in the urban and suburban areas of Xi'an City. They were examined by specialists and inquired for information on: Medical history and family medical history, alcohol intake, types of alcoholic beverage consumed, drinking patterns by detailed dietary questionnaires. Routine blood tests and ultrasonography were done.RESULTS: Multivariate analysis showed that: (1) The risk threshold for developing alcoholic liver disease was ingestion of more than 20 g alcohol per day, keeping on drinking for over 5 years in men. The highest OR was at the daily alcohol consumption ≥160 g, the occurrencerate of ALD amounted to 18.7% (P<0.01). No ALD occurred when ingestion of alcohol was less than 20 g per day. (2) 87.9% of all drank only at mealtimes. The cumulative risk of developing ALD was significantly higher in those individuals who regularly drank alcohol without food than in those who drank only at mealtimes, especially for those who regularly drank hard liquors only and multiple drinks (P<0.05). (3) The alcohol consumption in those with BMI ≥25 was lower than in those with BMI<25, but the risk increased to 11.5%, significantly higher than that of general population, 6.5% (P<0.01). (4) Abstinence and weight reduction could benefit the liver function recovery.CONCLUSION: In the Chinese population the ethanol risk threshold for developing ALD is 20 g per day, and this risk increases with increased daily intake. Drinking 20 g of ethanol per day and for less than 5 years are safe from ALD. Drinking alcohol outside mealtimes and drinking hard liquors only and multiple different alcohol beverages both increase the risk of developing ALD. Obesity also increases the risk, Abstinence and weight reduction will directly affect the prognosis of ALD, Doctor's strong advice might influence the prognosis indirectly. | Xiao-LanLu Jin-YanLuo MingTao YanGen PingZhao Hong-LiZhao Xiao-DongZhang NeiDong | 2004 | World Journal of Gastroenterology2004，10，16： | 17 |
| 3 | DNA Vaccine of SARS-Cov S Gene Induces Antibody Response in Mice显示文摘The spike (S) protein, a main surface antigen of SARS-coronavirus (SARS-CoV), is one of the most important antigen candidates for vaccine design. In the present study, three fragments of the truncated S protein were expressed in E.coli, and analyzed with pooled sera of convalescence phase of SARS patients.The full length S gene DNA vaccine was constructed and used to immunize BALB/c mice. The mouse serum IgG antibody against SARS-CoV was measured by ELISA with E.coli expressed truncated S protein or SARS-CoV lysate as diagnostic antigen. The results showed that all the three fragments of S protein expressed by E.coli was able to react with sera of SARS patients and the S gene DNA candidate vaccine could induce the production of specific IgG antibody against SARS-CoV efficiently in mice with seroconversion ratio of 75% after 3 times of immunization. These findings lay some foundations for further understanding the immunology of SARS-CoV and developing SARS vaccines. | PingZHAO Jin-ShanKE Zhao-LinQIN HaoREN Lan-JuanZHAO Jian-GuoYU | 2004 | Acta Biochimica et Biophysica Sinica2004，36，1： | 7 |
| 4 | Phage display selection on whole cells yields a small peptide specific for HCV receptor human CD81显示文摘The human CD81(hCD81),the most recently proposed receptor of hepatitis C virus(HCV),can especifically bind to HCV envelope glycoprotein 2(E2).In this study,hCD81-expressing murine NIH/3T3 cells were used to select hCD81-binding peptides from a phage displayed nonapeptide library(PVIII9aaCys).Eighteen of the 75clones selected from the library showed specific binding to the hCD81-expressing NIH/3T3 cells by enzyme linked immunosorbent assay(ELISA)and competitive inhibition test.Twelve out of the 18 clones shared the amino acid motif SPQYWTGPA.Sequence comparison of the motif showed no amino acid homology with the native HCV E2.The motif-containing phages could competitively inhibit the ability of HCV E2 binding to native hCD81-expressing MOLT-4 cells,and induce HCV E2 specific immune response in vivo.These results suggest that the selected motif SPQYWTGPA should be a mimotope of HCV E2 to bind to hCD81 molecules.Our findings cast new light on developing HCV receptor antagonists. | JIECAO PINGZHAO XIAOHUIMIAO LANJUANZHAO LIJUNXUE ZHONGTIANQI | 2003 | Cell Research2003，13，6： | 4 |
| 5 | Comparative Proteomic Study of Pancreatic Cancer and Hepatocellular Carcinoma Using Antibody Microarray显示文摘 | Xiao-GuangNI PingZHAO Jing-QunHU Xiao-FengBAI Yu-LinSUN FangLIU Lan-PingZHOU Shang-MeiLIU Zi-SenZHANG Xiao-HangZHAO | 2004 | Acta Biochimica et Biophysica Sinica2004，36，1： | 2 |
| 6 | MicroRNA-144 is a circulating effector of remote ischemic preconditioning显示文摘 | Jing Li Sagar Rohailla Nitai Gelber James Rutka Nesrin Sabah Rachel A. Gladstone Can Wei Pingzhao Hu Rajesh K. Kharbanda Andrew N. Redington | 2014 | Basic Research in Cardiology2014，，5： | 2 |
| 7 | Immunotoxins and Cancer Therapy显示文摘In the past decade, an increased amount of clinically-oriented research involving immunotoxins has been published.Immunotoxins are a group of artificially-made cytotoxic molecules targeting cancer cells. These molecules composed of a targeting moiety, such as a ligand or an antibody, linked to toxin moiety, which is a toxin with either truncated or deleted cell-binding domain that prevents it from binding to normal cells. Immunotoxins can be divided into two categories: chemically conjugated immunotoxins and recombinant ones. The immunotoxins of the first category have shown limited efficacy in clinical trials in patients with hematologic malignancies and solid tumors. Within the last few years, single-chain immunotoxins provide enhanced therapeutic efficacy over conjugated forms and result in improved antitumor activity. In this review, we briefly illustrate the design of the immunotoxins and their applications in clinical trials. Cellular & Molecular Immunology. 2005;2(2):106-112. | ZhengLi TaoYu PingZhao JieMa | 2005 | Cellular & Molecular Immunology2005，2，2： | 2 |
| 8 | Hepatitis B virus S gene enhances immune responses of a multiple-epitope DNA construct of hepatitis C virus in mice显示文摘The purpose of this study was to construct an eukaryotic DNA vector encoding a multiple-epitope antigen (MFC) of hepatitis C virus (HCV) and a hepatitis B surface antigen (HBsAg) , and explore the effect of HBsAg gene on the immunity of HCV multiple-epitope DNA construct in vitro and in vivo in mice. An HCV DNA vector (pVAX1-HBs-MFC) was constructed by fusing HBsAg gene to the N-terminal of an HCV multiple-epitope antigen gene. The pVAX1-HBs-MFC was transfected into HEK 293T cells and its expression was measured by ELISA and Western blotting. BALB/c mice were intramuscularly immunized with the pVAX1-HBs-MFC, and an ELISA approach was applied to determine the specific antibody titers and subtypes in the mouse serum. The cross-reactivity of the antibodies was also checked with two synthesized HCV hypervariable region 1 (HVR1) peptides. The IFN-γ production and cell proliferation of the mouse spleen cells were evaluated by ELISA and MTS (3-[4, 5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl] -2H-tetrazolium, inner salt) assays, respectively. The expression of pVAX1-HBs-MFC was detectable in the transfected HEK 293T cells. The serum antibody response was effectively elicited in BALB/c mice injected with pVAX1-HBs-MFC. The highest titer of antibody against HCV (MFC) was 1∶1280, and the ratio of IgG2a/IgG1 was 1.50±0.12 at the fifth week after first immunization. Moreover, the collected mouse serum antibody had the ability to cross-react with the two synthesized HCV HVR1 peptides. The stimulation index of the mouse splenocytes to MFC was 1.79 ±0.07, and the IFN-γ level was 287±6?pg/ml at week 21 after first immunization. The highest titer of the antibody in control BALB/c mice immunized with pVAX1-MFC was 1∶320, and the ratio of IgG2a/IgG1 was 1.33±0.11 at week 5 post-immunization. Furthermore, the stimulation index of the mouse splenocytes cells to MFC was 1.52±0.06, and the IFN-γ level was 225±9.3?pg/ml at week 21 post-immunization. The HBsAg gene can enhance the effects of an HCV multiple-epitope DNA construct on its humoral and cellular immune responses. This HBsAg enhanced HCV multiple-epitope DNA vector may be of potential use in the development of HCV vaccines. | YUPINGGONG JUNGAO PINGZHAO XIAOPINGYANG ZHONGTIANQI | 2005 | Journal of Microbiology and Immunology2005，3，1： | 1 |
| 9 | Utility of a physiologically–based pharmacokinetic (PBPK) modeling approach to quantitatively predict a complex drug–drug–disease interaction scenario for rivaroxaban during the drug review process: implications for clinical practice显示文摘 | Joseph A.Grillo PingZhao JulieBullock Brian P.Booth MinLu KathyRobie‐Suh Eva GilBerglund K. SandyPang AtiqurRahman LeiZhang Lawrence J.Lesko Shiew‐MeiHuang | 2012 | Drug Dispos2012，，2： | 1 |
| 10 | Activation of notch‐1 enhances epithelial–mesenchymal transition in gefitinib‐acquired resistant lung cancer cells显示文摘 | MianXie LiZhang Chao‐shengHe FeiXu Jun‐lingLiu Zhi‐huangHu Li‐pingZhao YingTian | 2012 | J. Cell. Biochem2012，，5： | 1 |
| 11 | Markers of survival and metastatic potential in childhood CNS primitive neuro-ectodermal brain tumours: an integrative genomic analysis显示文摘 | Daniel Picard Suzanne Miller Cynthia E Hawkins Eric Bouffet Hazel A Rogers Tiffany SY Chan Seung-Ki Kim Young-Shin Ra Jason Fangusaro Andrey Korshunov Helen Toledano Hideo Nakamura James T Hayden Jennifer Chan Lucie Lafay-Cousin Pingzhao Hu Xing Fan Karin | 2012 | Lancet Oncology2012，，8： | 1 |
| 12 | Utility of a physiologically–based pharmacokinetic (PBPK) modeling approach to quantitatively predict a complex drug–drug–disease interaction scenario for rivaroxaban during the drug review process: implications for clinical practice显示文摘 | Joseph A.Grillo PingZhao JulieBullock Brian P.Booth MinLu KathyRobie‐Suh Eva GilBerglund K. SandyPang AtiqurRahman LeiZhang Lawrence J.Lesko Shiew‐MeiHuang | 2012 | Biopharm Drug Dispos2012，，2： | 1 |
| 13 | Assessment of Resectability of Pancreatic Carcinoma by Endoscopic Ultrasonography显示文摘OBJECTIVE To study the value of endoscopic ultrasonography (EUS) in the preoperative assessment of resectability of pancreatic carcinoma. METHODS Thirty-eight non-selected consecutive patients were prospectively investigated using EUS to determine tumor resectability. The EUS findings in each of the patients were evaluated prospectively by 2 EUS specialists. All patients were explored and resectability determinded.RESULTS Ten out of 38 cases with pancreatic carcinoma were considered to be resectable by EUS with a positive predictive value of 80% compared to findings with surgery. EUS also had a high correlation with surgical results in assessing unresectability of pancreatic carcinoma, the negative predictive. value was 96.4%. There were 2 false-positive and 1 false-negative assessments (sensitivity, 88.9% and specificity, 93.1%). The accuracy of EUS was the highest. The diagnostic accuracy of EUS, CT, MRI and sonography were 97.4%, 94.6%, 89.5%, and 73.6% respectively. CONCLUSION Endoscopic ultrasonography is an effective method to assess resectability of pancreatic carcinoma. | YantaoTian PingZhao ChengfengWang GuiqiWang JianxiongWu YiShan YongfuShao | 2004 | Chinese Journal of Clinical Oncology2004，1，6： | 0 |
| 14 | PI3K activation is enhanced by FOXM1D binding to p110 and p85 subunits显示文摘Dear Editor,Class I PI3Ks play a central role in cancer progression via its downstream signaling nodes(GSK3,FOXO,mTORC1,etc.).1 Under physiological conditions,the catalytic p110 subunit is stabilized and inhibited by the regulatory p85 subunit in the cytoplasm.1,2 In cancer cells,PI3K activity is aberrantly regulated mostly through excessive upstream growth signals,disinhibition of p110 by genetic alteration of PI3K genes,and deficiency of the phosphatase PTEN.1 Protein interactions between PI3K and overexpressed oncoproteins such as Ras also account for the activation of PI3K in cancer cells.1 Abnormally activated PI3K/AKT pathway is a popular drug target for cancer therapy;therefore,the mechanisms underlying the regulation of PI3K activity need the elaborate exploration. | Qi Wang Pingzhao Zhang Wei Zhang Xin Zhang Jianfeng Chen Peipei Ding Luying Li Xinyue Lv Ling Li Weiguo Hu | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 0 |
| 15 | Hepatitis G virus genomic RNA is pathogenic to Macaca mulatta显示文摘AIM: To explore the pathogenicity and infectivity of hepatitis G virus (HGV) by observing replication and expression of the virus, as well as the serological and histological changes of Macaca mulatta infected with HGV genomic RNA or HGV RNA-positive serum.METHODS: Full-length HGV cDNA clone (HGVqz) was constructed and proved to be infectious, from which HGV genomic RNA was transcribed in vitro. Macaca mulatta BY1 was intra-hepatically inoculated with HGV genomic RNA, HGV RNA-positive serum from BY1 was intravenously inoculated into Macaca mulatta BM1, and then BB1 was infected with serum from BM1. Serum and liver tissue were taken regularly, and checked with RT-PCR, in situ hybridization and other immunological, serological,histological assays.RESULTS: Serum HGV RNA was detectable in all the 3Macaca mulattas, serological and histological examinations showed the experimental animals had slightly elevated alanine transaminase (ALT) and developed HGV viremia during the infectious period. The histology, immunohistochemistry, and in situ hybridization in liver tissues of the inoculated animals demonstrated a very mild hepatitis with HGV antigen expression in cytoplasm of hepatocytes.RT-PCR and quantitative PCR results showed that HGV could replicate in liver.CONCLUSION: The genomic RNA from full-length HGV cDNA is infectious to the Macaca mulatta and can cause mild hepatitis. HGV RNA-positive serum, from HGV RNA inoculated Macaca mulatta, is infectious to other Macaca mulattas. Macaca mulatta is susceptible to the inoculated HGV, and therefore can be used as an experimental animal model for the studies of HGV infection and pathogenesis. | HaoRen Fen-LuZhu Ming-MeiCao Xin-YuWen PingZhao Zhong-TianQi | 2005 | World Journal of Gastroenterology2005，11，7： | 0 |
| 16 | Anodic Cyanation of 1-(1-Methoxycarbonyl ethyl) piperidine显示文摘Aminonitriles were prepared efficiently from anodic cyanation of ?amino ester. The effect of different solvents and electrolytes was studied. The other byproducts were analyzed. | PingZHAO YingWuYIN | 2004 | Chinese Chemical Letters2004，15，9： | 0 |
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