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| 1 | Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro显示文摘Purified avian infectious bronchitis virus (IBV) was used to screen a random phage dis-play peptide library. After the fourth panning, 10 positive phages were sequenced and characterized. The phages specifically inhibited IBV infectivity in HeLa cells and blocked IBV haemagglutination. One linear peptide “GSH HRH VHS PFV” from the positive phages with the highest neutralization titer was synthesized and this peptide inhibited IBV infection in HeLa as well. The results may contribute to development of antiviral therapeutics for IBV and studying the determinants for viral and cell interac-tion. | PENG Bo1, CHEN Hanyang1, TAN Yadi1,2, JIN Meilin1,2, CHEN Huanchun1,2 & GUO Aizhen1,2 1. Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China 2. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China | 2006 | Science China(Life Sciences)2006，49，2： | 3 |
| 2 | Screening of hepatocyte proteins binding to complete S protein of hepatitis B virus by yeast-two hybrid system显示文摘AIM: To investigate the biological function of complete S protein and to look for proteins interacting with complete S protein in hepatocytes.METHODS: We constructed bait plasmid expressing complete S protein of HBV by cloning the gene of complete S protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (a type). The transformed yeast AH109 was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2xYPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/Trp-Leu-His-Ade) containing X-α-gal for selection and screening. After extracting and sequencing of plasmids from positive (blue) colonies, we underwent sequence analysis by bioinformatics.RESULTS: Nineteen colonies were selected and sequenced.Among them, five colonies were Homo sapiens solute carrier family 25, member 23 (SLC25A23), one was Homo sapiens calreticulin, one was human serum albumin (ALB)gene, one was Homo sapiens metallothionein 2A, two were Homo sapiens betaine-homocysteine methyltransferase,three were Homo sapiensNa+ and H+ coupled amino acid transport system N, one was Homo sapiens CD81 antigen (target of anti-proliferative antibody 1) (CD81), three were Homo sapiens diazepam binding inhibitor, two colonies were new genes with unknown function.CONCLUSION: The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with complete S protein of HBV. The complete S protein may bind to different proteins i.e., its multiple functions in vivo. | Gui-QinBai JunCheng Shu-LinZhang Yan-PingHuang LinWang YanLiu Shu-MeiLin | 2005 | World Journal of Gastroenterology2005，11，25： | 1 |
| 3 | 四次跨膜蛋白CD81在免疫性疾病中的作用显示文摘CD81是一种具有多种生物学活性的四次跨膜蛋白,几乎广泛表达于人体的所有组织。CD81参与蛋白合成转运及激活、微绒毛的形成与延伸、淋巴细胞免疫活化等多种生理反应过程,在病原体感染、过敏性皮炎、动脉粥样硬化、脑脊髓炎、肿瘤、先兆子痫、系统性红斑狼疮等免疫性疾病中发挥重要作用。文章概述了CD81的分子结构、生物功能及其在免疫性疾病中的最新研究进展。 | 杨子锐 马禹良 王玉竹 窦环 | 2022 | 国际免疫学杂志2022，45，3： | 0 |
| 4 | 体外抑制鸡传染性支气管炎病毒的多肽鉴定显示文摘使用纯化的鸡传染性支气管炎病毒(IBV),从12肽噬菌体随机展示肽库中筛选特异性结合多肽.经过四轮淘筛,得到10个阳性噬菌体,进一步进行测序、血凝抑制活性及病毒抑制特性鉴定.所有阳性噬菌体均能特异性阻断IBV对HeLa细胞的感染,并能抑制IBV对鸡红细胞的血凝活性.人工合成其中一条病毒抑制效价最高的线性多肽“GSH HRH VHS PFV”,其细胞抑制试验证实该多肽同样具有病毒抑制特性.以上结果为研制抗病毒分子及鉴定病毒与细胞相互作用功能域等奠定了基础. | 彭博 陈汉阳 谭亚娣 金梅林 陈焕春 郭爱珍 | 2005 | 中国科学(C辑)2005，35，6： | 0 |
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