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| 1 | Overexpression of annexin 1 in pancreatic cancer and its clinical significance显示文摘AIM: To investigate the expression of annexin I in pancreatic cancer and its relationship with the clinicopathologic factors,and to evaluate its potential clinical significance.METHODS: Annexin I expression was analyzed by Western blot and immunohistochemical staining in pancreatic adenocarcinoma and multi-tissue microarrays (MTAs).RESULTS: Western blot analysis showed that annexin I was overexpressed in 84.6% (11/13) pancreatic ductal adenocarcinomas. Immunohistochemistry analysis of pancreatic cancer in MTAs showed that annexin I protein was 71.4%(30/42) positive which was markedly increased compared with that in the tumor matched normal pancreas tissues 18.4%(7/38) (P<0.01). In the meantime, the high expression of annexin 1 was correlated with the poor differentiation of pancreatic adenocarcinoma.CONCLUSION: Annexin 1 overexpression is a frequent biological marker and correlates with the differentiation of pancreatic cancer during tumorigenesis. | Xiao-FengBai Xiao-GuangNi PingZhao Shang-MeiLiu Hui-XinWang BingGuo Lan-PingZhou FangLiu Jin-ShengZhang KunWang Yong-Qiangxie Yong-FuShao Xiao-HangZhao | 2004 | World Journal of Gastroenterology2004，10，10： | 40 |
| 2 | Loss of clusterin both in serum and tissue correlates with the tumorigenesis of esophageal squamous cell carcinoma via proteomics approaches显示文摘AIM: To identify the differentially secreted proteins or polypeptides associated with tumorigenesis of esophageal squamous cell carcinoma (ESCC) from serum and to find potential tumor secreted biomarkers.METHODS: Proteins from human ESCC tissue and its matched adjacent normal tissue; pre-surgery and postsurgery serum; and pre-surgery and normal control serum were separated by two-dimensional electrophoresis (2-DE)to identify differentially expressed proteins. The silverstained 2-DE were scanned with digital ImageScanner and analyzed with ImageMaster 2D Elite 3.10 software. A cluster of protein spots differentially expressed were selected and identified with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). One of the differentially expressed proteins, clusterin, was downregulated in cancer tissue and pre-surgery serum, but it was reversed in post-surgery serum. The results were confirmed by semi-quantitative reverse-transcription (RT)-PCR and western blot.RESULTS: Comparisons of the protein spots identified on the 2-DE maps from human matched sera showed that some proteins were differentially expressed, with most of them showing no differences in composition, shape or density.Being analyzed by MALDI-TOF-MS and database searching,clusterin was differentially expressed and down-regulated in both cancer tissue and pre-surgery serum compared with their counterparts. The results were also validated by RTPCR and western blot.CONCLUSION: The differentially expressed clusterin may play a key role during tumorigenesis of ESCC. The 2DE-MS based proteomic approach is one of the powerful tools for discovery of secreted markers from peripheral. | Li-YongZhang Wan-TaoYing You-ShengMao Hong-ZhiHe YuLiu Hui-XinWang FangLiu KunWang De-ChaoZhang YingWang MinWu Xiao-HongQian Xiao-HangZhao | 2003 | World Journal of Gastroenterology2003，9，4： | 26 |
| 3 | Effects of Fluoride on Lipid Peroxidation, DNA Damage and Apoptosis in Human Embryo Hepatocytes显示文摘Objective To investigate the effects of fluoride on lipid peroxidation, DNA damage and apoptosis in human embryo hepatocyte L-02 cells. Methods Lipid peroxide (LPO) level, reduced glutathione (GSH) content, DNA damage, apoptosis, and cell cycle analysis were measured after in vitro cultured L-02 cells were exposed to sodium fluoride at different doses (40 μg/mL, 80 μg/mL, and 160 μg/mL) for 24 hours. Results Fluoride caused an increase of LPO levels and a decrease of GSH content in L-02 cells. There appeared to be an obvious dose-effect relationship between the fluoride concentration and the observed changes. Fluoride also caused DNA damage and apoptosis and increased the cell number in S phase of cell cycle in the cells tested. There was a statistically significant difference in DNA damage and apoptosis when comparing the high dose of fluoride treated cells with the low dose of fluoride treated cells. Conclusion Fluoride can cause lipid peroxidation, DNA damage, and apoptosis in the L-02 cell experimental model and there is a significant positive correlation between fluoride concentration and these pathological changes. | AI-GuoWANG TAOXIA QI-LONGCHU MINGZHANG FANGLIU XUE-MINCHEN KE-DIYANG | 2004 | Biomedical and Environmental Sciences2004，17，2： | 18 |
| 4 | RNA interference by expression of short hairpin RNAs suppresses bcl-xL gene expression in nasopharyngeal carcinoma cells显示文摘Aim: To evaluate a new plasmid mediated RNA interference (RNAi) system and investigate whether knock-down of bcl-xL by short hairpin RNA (shRNA) can induce apoptosis of human nasopharyngeal carcinoma (NPC) cell line CNE-2Z in vitro. Methods: The plasmid containing mU6 promoter was subcloned to yield the pmU6 plasmid, recombinant plasmid expressing shRNA targeting bcl-xL gene was designed and constructed, and were co-transfected cells with green fluorescence protein expressing plasmid. Flow cytometry was used to evaluate transfection efficiency, and RT-PCR and Western blot were applied to analyze bcl-xL mRNA and protein levels, respectively. Results: The shRNA expressed by the recombinant plasmid efficiently suppressed bcl-xL gene expression and induced apoptosis of NPC cells in vitro. Conclusion: The recombinant plasmid can sufficiently mediate RNAi in CNE-2Z cells, and knock-down of the bcl-xL expression by shRNA significantly induced apoptosis in CNE-2Z cells. The results suggest this new system, mediated RNAi can be used as a tool for the study of gene function and gene therapy. | FangLIU Cheng-weiHE Yue-feiZHANG Ke-yuanZHOU | 2005 | Acta Pharmacologica Sinica2005，26，2： | 18 |
| 5 | Identification and characterization of neuroblasts in the subventricular zone and rostral migratory stream of the adult human brain显示文摘在成年人的前脑遗体在 subventricular 地区(SVZ ) 和象小径一样的喙的迁移的溪流(RMS ) 的存在在神经发生的成年人的大脑,而是坚持识别新神经原兴趣大高度争论。在现在的学习,我们在成年猴子,胎儿的人和成年人的大脑描述了 RMS 的一般配置。我们提供神经母细胞在成年人的大脑的前面的腹的 SVZ 和 RMS 连续地存在的证据。没有形成链,神经母细胞单身地或在对出现;他们展出迁移的形态学和合作快车不成熟的 neuronal 标记 doublecortin, polysialylated 神经房间粘附分子和 β III 导管素。很少这些神经母细胞看起来活跃地在前面的腹的 SVZ 正在增殖,显示沿着 RMS 散布的神经母细胞是最可能的但是任何一个都没在 RMS 源于腹的 SVZ。有趣地,没有神经母细胞在成年人的嗅觉的灯泡被发现。一起拿,我们的数据建议 SVZ 维持能力在成年人的大脑生产神经母细胞。 | CongminWang FangLiu Ying-YingLiu Cai-HongZhao YanYou LeiWang JingxiaoZhang BinWei TongMa QiangqiangZhang YueZhang RuiChen HongjunSong ZhengangYang | 2011 | Cell Research2011，21，11： | 13 |
| 6 | Interleukin-10 modified dendritic cells induce allo-hyporesponsiveness and prolong small intestine allograft survival显示文摘AIM: To investigate whether TL-10-transduced dendritic cells (DCs) could induce tolerogenicity and prolong allograft survival in rat intestinal transplantation.METHODS: Spleen-derived DCs were prepared and genetically modified by hTL-10 gene. The level of IL-10 expression was quantitated by ELTSA. DC function was assessed by MTT in mixed leukocyte reaction. Allogeneic T-cell apoptosis was examined by flow cytometric analysis. Seven days before heterotopic intestinal transplantation, 2x106 donor-derived IL-10-DC were injected intravenously, then transplantation was performed between SD donor and Wistar recipient.RESULTS: Compared with untransduced DC, IL-10-DC could suppress allogeneic mixed leukocyte reaction (MLR). The inhibitory effect was the most striking with the stimulator/effector (S/F) ratio of 1:10. The inhibition rate was 33.25 %,41.19 % (P<0.01) and 22.92 % with the S/E ratio of 1:1,1:10 and 1:50 respectively. At 48 hours and 72 hours by flow cytometry counting, apoptotic T cells responded to IL-10-DC in MLR were 13.8 % and 30.1%, while untransduced group did not undergo significant apoptosis (P<0.05). IL-10-DC pretreated recipients had a moderate survival prolongation with a mean allograft survival of 19.8 days (P<0.01),compared with 7.3±2.4 days in control group and 8.3±2.9days in untransduced DC group. Rejection occurred in the control group within three days. The difference between untreated DC group and control group was not significant.CONCLUSION: IL-10-DC can induce allogenic T-cell hyporesponsiveness in vitro and apoptosis may be involved in it. IL-10-DC pretreatment can prolong intestinal allograft survival in the recipient. | MinZhu Ming-FaWei FangLiu Hui-FenShi GuoWang | 2003 | World Journal of Gastroenterology2003，9，11： | 10 |
| 7 | Translocation of annexin I from cellular membrane to the nuclear membrane in human esophageal squamous cell carcinoma显示文摘AIM: To investigate the alteration of the annexin I subcellular localization in esophageal squarnous cell carcinoma (ESCC)and the correlation between the translocation and the tumorigenesis of ESCC.METHODS: The protein localization of annexin I was detected in both human ESCC tissues and cell line via the indirect immunofluorescence strategy.RESULTS: In the normal esophageal epithelia the annex in I was mainly located on the plasma membrane and formed a consecutive typical trammels net. Annexin I protein also expressed dispersively in cytoplasm and the nuclei without specific localization on the nuclear membrane. In esophageal cancer annexin I decreased very sharply with scattered disappearance on the cellular membrane, however it translocated and highly expressed on the nuclear membrane,which was never found in normal esophageal epithelia. In cultured esophageal cancer cell line annexin I protein was also focused on the nuclear membrane, which was consistent with the result from esophageal cancer tissues.CONCLUSION: This observation suggests that the translocation of annexin I protein in ESCC may correlate with the tumorigenesis of the esophageal cancer. | YuLiu Hui-XinWang NingLu You-ShengMao FangLiu YingWang Hai-RongZhang KunWang MinWu Xiao-HangZhao | 2003 | World Journal of Gastroenterology2003，9，4： | 10 |
| 8 | Inhibition of mouse hepatocyte apoptosis via anti-Fas ribozyme显示文摘AIM: TO investigate the effects of anti-Fas ribozyme on Fas expression and apoptosis in primary cultured mouse hepatocytes.METHODS: Mouse hepatocytes were isolated by using collagenase irrigation. A hammerhead ribozyme targeting the Fas mRNA was constructed, and transfected into mouse hepatocytes via Effectene. Then Fas expression in mouse hepatocytes was detected by RT-PCR and western blotting.After being treated with anti-Fas antibody (JO2), hepatocytes viability was measured with MTI- assay. Caspase-3 proteolytic activity was detected, and cell apoptosis was measured according to Annexin V-FITC apoptosis detection kit.RESULTS: Fas expressed in primary mouse hepatocytes.Fas expression in hepatocytes transfected with anti-Fas ribozyme was decreased remarkably and correlated with the resistance to Fas-mediated apoptosis as determined by flow cytometry and caspase-3 proteolytic activity.CONCLUSION: AnU-Fas ribozyme can remarkably decrease the Fas expression in mouse hepatocytes, thus inhibit Fasmediated apoptosis in hepatocytes, It is suggested that anti-Fas ribozyme could significantly increase the resistance of transplanted hepatocytes to apoptosis and improve the survival of transplanted hepatocytes, | MinZhang WeiHe FangLiu PingZou JuanXiao Zhao-DongZhong Zhong-BoHu | 2004 | World Journal of Gastroenterology2004，10，17： | 8 |
| 9 | Alterations in expression,proteolysis and intracellular localizations of clusterin in esophageal squamous cell carcinoma显示文摘AIM: To investigate biogenesis and intracellular Iocalizations of clusterin to elucidate the potential molecular mechanisms implicated in tumorigenesis of esophageal mucosa.METHODS: Semi-quantitative RT-PCR for multi-region alteration analysis, Western blot for different transcriptional forms and immunohistochemical staining for intracellular Iocalizations of clusterin were carried out in both tissues and cell lines of ESCC.RESULTS: The N-terminal deletions of the clusterin gene and the appearance of a 50-53 ku nuclear clusterin, an uncleaved, nonglycosylated, and disulfide-linked isoform,were the major alterations in cancer cells of esophagus.Naturally the 40 ku clusterin was located in the connective tissue of the lamina propria of epithelial mucosa and right under the basal membrane of epithelia, but it was disappeared in stromal mucosa of esophagus and the pre-matured clusterin was found positive in cancerous epithelia.CONCLUSION: The N-terminal deletion of clusterin may be essential for its alterations of biogenesis in ESCC. | Hong-ZhiHe Zhen-MeiSong KunWang Liang-HongTeng FangLiu You-ShengMao NingLu Shang-ZhongZhang MinWu Xiao-HangZhao | 2004 | World Journal of Gastroenterology2004，10，10： | 7 |
| 10 | Genesis of Yangla Banded Skarn-Hosted Copper Deposit in Tethys Orogenic Belt of Southwestern China显示文摘INTRODUCTIONTheYanglacopperdepositishostedbybandedskarn,whichwastraditionalyconsideredtohavebeenformedbycontactmetasomaticpro... | Zhan Mingguo Lu Yuanfa Yichang Institute of Geology and Mineral Resources, CAGS, Yichang 443003 Dong Fangliu Department of Geology and Mineral Resources, China University of Geosciences, Beijing 100083 Chen Kaixu Wei Junqi Yichang Institute of | 1999 | Journal of Earth Science1999，18，1： | 6 |
| 11 | Gender differences in hepatic ischemic reperfusion injury in rats are associated with endothelial cell nitric oxide synthase-derived nitric oxide显示文摘AIM: This study was designed to examine the hypothesis that gender differences in I/R injury are associated withendothelial cell nitric oxide synthase (eNOS)-derived nitric oxide (NO).METHODS: Wistar rats were randomized into seven experimental groups (12 animals per group). Except for the sham operated groups, all rats were subjected to total liver ischemia for 40 min followed by reperfusion. All experimental groups received different treatments 45 min before the laparotomy. For each group, half of the animals (six) were used to investigate the survival; blood samples and liver tissues were obtained in the remaining six animals after 3 h of reperfusion to assess serum NO, alanine aminotransferase (ALT) and TNF-α levels, liver tissuemalondialdehyde (MDA) content, and severity of hepatic I/R injury.RESULTS: Basal serum NO levels in female sham operated (FS) group were nearly 1.5-fold of male sham operated (MS) group (66.7±11.0 μmol/L vs 45.3±10.1μmol/L, P<0.01). Although serum NO levels decreased significantly after hepatic I/R (P<0.01, vs sham operated groups), they were still significantly higher in female rat (F) group than in male rat (M) group (47.8±8.6 μmol/L vs 23.8±4.7 μmol/L, P<0.01). Serum ALT and TNF-α levels, and liver tissue MDA content were significantly lower in F group than in M group (370.5±46.4 U/L, 0.99±0.11 μg/L and 0.57±0.10 μmol/g vs668.7±78.7 U/L, 1.71±0.18 μg/Land 0.86±0.11 μmol/g, respectively, P<0.01). I/R induced significant injury to the liver both in M and F groups (P<0.01 vs sham operated groups). But the degree of hepatocyte injury was significantly milder in F group than in M group (P<0.05 and P<0.01). The median survival time was six days in F group and one day in M group. The overall survival rate was significantly higher in F group than in M group (P<0.05). When compared with male rats pretreated with saline (M group), pretreatment of male rats with 17-β- estradiol (E2) (M+E2 group) significantly increased serum NO levels and significantly decreased serum ALT and TNF-α levels, and liver tissue MDA content after I/R (P<0.01).The degree of hepatocyte injury was significantly decreased and the overall survival rate was significantly improved in M+E2 group than in M group (P<0.01 and P<0.05). TheNOS inhibitor Nw-nitro-L-arginine methyl ester (L-NAME) treatment could completely abolish the protective effects of estrogen in both male and female rats. CONCLUSION: The protective effects afforded to female rats subjected to hepatic I/R are associated with eNOSderived NO. | PingLu FangLiu Chun-YouWang Dao-DaChen ZhongYao YuanTian Jing-HuiZhang Yi-HuaWu | 2005 | World Journal of Gastroenterology2005，11，22： | 6 |
| 12 | TACE Combined with PSE in Treating Hepatocelluar Carcinoma with Hypersplenism显示文摘OBJECTIVE To observe the effectiveness and safety of transcatheter arterial chemoembolization (TACE) combined with partial splenic embolization (PSE) in treating primary hepatocelluar carcinoma (HCC) with hypersplenism. METHODS Thirty HCC patients with liver cirrhosis, portal hypertension and hypersplenism were treated with TACE and PSE. The degree of tumor volume reduction and remission of hypersplenism were observed. RESULTS The tumor reduction rate of the HCC was 73.3%. Twenty-eight patients had hypersplenic remission with a rate of 93.3%. There were no severe complications such as hepatic abscesses. CONCLOUSION TACE combined with PSE is a safe and effective method to treat HCC with liver cirrhosis, portal hypertension and hypersplenism. | XiuyingGuo ZhiGuo HaipengYu FangLiu JunyiZhang JizhongXing | 2005 | Chinese Journal of Clinical Oncology2005，2，3： | 5 |
| 13 | Inhibition of Fas-mediated Apoptosis in Yac-1 Cell via Anti-Fas Ribozyme显示文摘To detect a new and more effective way against apoptosis mouse lymphomatic cell line- Yac-1 in which fas gene was expressed highly was used as a model for studying the effects of anti-Fas ribozyme on Fas-mediated apoptosis. A hammerhead ribozyme gene targeting the fas mRNA was synthesized and its in vitro transcription vector was constructed, which was transfected into Yac-1 cells using electroporation. Rz596 expression was detected using RT-PCR, and Fas expression in Yac-1 cells was detected using RT-PCR, Western blot and flow cytometry. After treated with anti-Fas antibody (JO2), Yac-1 cell viability was measured with MTT assay, caspase-3 proteolytic activity was detected, and cell apoptosis was measured according to annexin V apoptosis detecting kit. Anti-Fas ribozyme could cleave fas mRNA efficiently in vivo and in vitro. Fas expression in Yac-1 cells transfected with anti-Fas ribozyme was decreased remarkably and correlated with resistance to Fas-mediated apoptosis as determined by flow cytometry and caspase-3 proteolytic activity. Anti-Fas ribozyme was detected in cells transfected with pU6-RZ596 and pU6-dRZ596 and could remarkably decrease the Fas expression in Yac-1 cells, which made Yac-1 cells get rid of Fas-mediated apoptosis. Because of wide expression of fas in organs and tissues, our research was very useful for studying the inhibition of apoptosis of many organs and tissues in the future. | MinZHANG FangLIU WeiHE YongYOU PingZOU Ling-BoLIU | 2004 | Acta Biochimica et Biophysica Sinica2004，36，3： | 3 |
| 14 | Comparative Proteomic Study of Pancreatic Cancer and Hepatocellular Carcinoma Using Antibody Microarray显示文摘 | Xiao-GuangNI PingZHAO Jing-QunHU Xiao-FengBAI Yu-LinSUN FangLIU Lan-PingZHOU Shang-MeiLIU Zi-SenZHANG Xiao-HangZHAO | 2004 | Acta Biochimica et Biophysica Sinica2004，36，1： | 2 |
| 15 | Relationship between polymorphism of class Ⅱ transactivator gene promoters and chronic hepatitis B显示文摘AIM: To investigate the relationship between the polymorphism of class Ⅱ transactivator (CⅡTA) gene promoters and chronic hepatitis B (CHB).METHODS: Genomic DNA was prepared from peripheral blood leukocytes. Promoters Ⅰ, Ⅲ and Ⅳ of gene were analyzed respectively with polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) in 65 patients with CHB, 26 patients with acute hepatitis B (AHB) and 85 normal controls.RESULTS: No abnormal migration was found in PCR-SSCP analysis of the three promoters in the three groups. Also,no sequential difference was observed at the three promoters among the CHB patients, AHB patients and normal controls.CONCLUSION: No polymorphism in promoters Ⅰ, Ⅲ and Ⅳ of CⅡTA gene exists in CHB patients, ABH patients and normal controls, suggesting that the promoter of CⅡTA gene might be a conserved domain. | Ying-RenZhao LingGong Ying-LiHe FangLiu ChangLu | 2005 | World Journal of Gastroenterology2005，11，6： | 2 |
| 16 | OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice显示文摘 | FangLiu ZhiyeWang HongyanRen ChenjiaShen YeLi Hong‐QingLing ChangyinWu XingmingLian PingWu | 2010 | The Plant Journal2010，，3： | 1 |
| 17 | Nanoparficle-based adjuvant for enlmmed protective efficacy of DNA vaccine Ag85A-ESAT-6- IL-21 against Mycobacterium tuberculosis infection显示文摘 | Fangliu Yu | 2012 | Nanotechnology Biology and Medicine:2012，8，8： | 1 |
| 18 | High temperature oxidation behavior of high speed steel for hot rolls material显示文摘The oxidation characteristics of high speed steel (HSS) were studied at 500 to 800°C. The non-isothermal oxidation and isothermal oxidation (500, 575, 650, 725, 800°C) of HSS were investigated by thermo-gravimetric analysis (TGA). The microstruc- ture, morphology and oxide scale thickness of the isothermal oxidation samples were analyzed by optical microscope (OM), electron probe micro analyzer (EPMA), X-ray diffraction spectrum (XRD) and scanning electron microscope (SEM). The results indicate that the oxidation rate of HSS is very slow at 500 to 650°C, increasing gradually at 650 to 750°C, and drastically at 750 to 800°C, be- cause the phase transformation happens at about 750°C. | LiZhou FangLiu ChangshengLiu DaleSun LisongYao | 2005 | Journal of University of Science and Technology Beijing2005，12，2： | 1 |
| 19 | Analysis of chemical components in oolong tea in relation to perceived quality显示文摘 | KunboWang FangLiu ZhongxiXu YinhuaLi JinhuaChen YushunGong XingheYang | 2010 | International Journal of Food Science & Technology2010，，5： | 1 |
| 20 | CONSTRUCTION AND IMMUNOGENICITY OF HUMAN PAPILLOMAVIRUS TYPE 6B L1 RECOMBINANT PLASMID显示文摘To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immu-nogenicity in mice. Methods The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic ex-pression plasmid (pcDNA3.1). The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunoge-nicity study. Results The recombinant plasmid (pcDNA3.1-HPV6bL1) was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice’s sera inoculated with pcDNA3.1-HPV6bL1. Similarly, IL-4, IL-2, and IFN-γ were increased in the same mice. Conclusion HPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata..- | FangLiu Jia-biWang Ya-gangZuo Yue-huaLiu Dong-laiMa | 2004 | Chinese Medical Sciences Journal2004，19，3： | 1 |
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