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| 1 | Fen1 mutations that specifically disrupt its interaction ~vith PCNA cause aneuploidy-associated cancer显示文摘 | Li Zheng Huifang Dai Muralidhar L Hegde Mian Zhou Zhigang Guo Xiwei Wu Jun Wu Lei Su Xueyan Zhong Sankar Mitra Qin Huang Kemp H Kernstine Gerd P Pfeifer Binghui Shen | 2011 | Cell Research2011，21，7： | 3 |
| 2 | 集成多测量技术的自动检测系统显示文摘介绍了现有接触式测量技术与非接触式光学测量技术的优缺点。为了实现工业产品的高精度高效测量,提出了一种新型的自动检测系统。此系统在三坐标测量机上集成了视觉系统和锥光偏振全息光学测量系统,并可根据不同的精度要求和零件结构选择适当的测量装置。文章介绍了系统的总体方案以及核心模块。 | 陈华成 王伯雄 罗秀芝 Li Z. Pfeifer T. | 2005 | 机械科学与技术2005，24，5： | 3 |
| 3 | Protein phosphatase PP4 is overexpressed in human breast and lung tumors显示文摘 | Bo Wang Ailian Zhao Lingling Sun Xueyan Zhong Jianing Zhong Haibo Wang Minhua Cai Jing Li Yi Xu Ji Liao Jianli Sang Dipanj an Chowdhury Gerd P Pfeifer Yun Yen Xingzhi Xu | 2008 | Cell Research2008，18，9： | 2 |
| 4 | Gross Motor Function Classification System Expanded & Revised (GMFCS E & R):reliability between therapists and parents in Brazil显示文摘 | Silva DB Pfeifer LI Funayama CA | 2013 | Braz J Phys Ther2013，17，5： | 1 |
| 5 | Classifica-tion of cerebral palsylassociation between gender,age,motortype,topography and Gross Motor Function显示文摘 | Pfeifer LI Silva DB Funayama CA et a1 | 2009 | Arq Nen-ropslquiatr2009，67，4： | 1 |
| 6 | Crystallite-pore network model of transport and reaction of multicomponent gas mixtures in polycrystalline microporous media显示文摘 | Ding W J Li Hui Pfeifer P | 2014 | Chemical Engineering Journal2014，254，： | 1 |
| 7 | Social support provided to caregivers of children with cerebral palsy 显示文摘 | Pfeifer LI Silva DB Lopes PB | 2014 | Child Care Health Dev2014，40，3： | 1 |
| 8 | Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation显示文摘 | Jia J Zhao SC Kong XY Li YR Zhao GY He WM Appels RD Pfeifer M Tao Y Zhang XY Jing RL Zhang C Ma YZ Gao LF Gao C Spannagl M Mayer KFX Li D Pan SK Zheng FY Hu Q Xia XC Li JW Liang QS Chen J Wicker T Gou CY Kuang HH He GY Luo YD Keller B Xia QJ Lu P Wang JY | | 0，，： | 1 |
| 9 | Classification of cerebral palsy : Association between gender, age, motor type, topography and gross motor function显示文摘 | Pfeifer LI Silva DB Funayama CA | 2009 | Arq Neuropsiquiatr2009，67，4： | 1 |
| 10 | Social support provided to caregivers of children with cerebral palsy显示文摘 | Pfeifer LI Silva DB Lopes PB | 2013 | Child Care Health Dev2013，40，3： | 1 |
| 11 | Current and emerging options for taxol production显示文摘 | LI Y ZHANG G PFEIFER B A | 2015 | Adv Biochem Eng Biotechnol2015，148，： | 1 |
| 12 | Expression and localization of GRP75 in human epithelial tumors and normal tissues显示文摘 | Srokowski T Pfeifer JD Li J | 2004 | Appl Immunohistochem Mol Morphol2004，12，2： | 1 |
| 13 | Social support provided to caregivers of children with cerebral palsy 显示文摘 | Pfeifer LI Silva DB Lopes PB | 2013 | Child Care Health Dev2013，40，3： | 1 |
| 14 | Classification of cerebral palsy:association between gender,age,motor type,topography and Gross Motor Function显示文摘 | Pfeifer LI Silva DB Funayama CA | 2009 | Arq Neuropsiquiatr2009，67，4： | 1 |
| 15 | Classification of cerebral palsy:Association between gender,age,motor type,topography and Gross Motor Function显示文摘 | Pfeifer LI Silva DBR Funayama CAR | 2009 | Arq Neuropsiquiatr2009，7，4： | 1 |
| 16 | sea-derived fungus Acremonium sp.HDN16-126显示文摘Acremonium species are prolific producers of therapeutic molecules which include the widely used beta-lactam antibiotic,cephalosporin.In light of their significant medical value,an efficient gene disruption method is required for the physiological and biochemical studies on this genus of fungi.However,the number of selection markers that can be used for gene targeting is limited,which constrain the genetic analysis of multiple functional genes.In this study,we established a uridine auxotrophy based marker recycling system which achieves scarless gene deletion,and allows the use of the same selection marker in successive transformations in a deep seaderived fungus Acremonium sp.HDN16-126.We identified one homologue of Acremonium chrysogenum pyrG(also as a homologous gene of the yeast URA3)from HDN16-126,designated as pyrG-A1,which can be used as a selection marker on uridine free medium.We then removed pyrG-A1 from HDN16-126 genome via homologous recombination(HR)on MM medium with 5-fluoroortic acid(5-FOA),a chemical that can be converted into a toxin of 5-flurouracil by pyrG-A1 activity,thus generating the HDN16-126-△pyrG mutant strain which showed auxotrophy for uridine but insensitivity to 5-FOA and enabled the use of exogenous pyrG gene as both positive and negative selection marker to achieve the scarless deletion of target DNA fragments.We further applied this marker recycling system to successfully disrupt two target genes pepL(encodes a putative 2OG-Fe(Ⅱ)dioxygenase)and pepM(encodes a putative aldolase)identified from HDN16-126 genome,which are proposed to be functional genes related to 2-aminoisobutyric acid metabolism in fungi.This work is the first application of uridine auxotrophy based scarless gene deletion method in Acremonium species and shows promising potential in assisting sequential genetic analysis of filamentous fungi. | Ruonan Sun Hengyi Xu Yanyan Feng Xuewen Hou Tianjiao Zhu Qian Che Blaine Pfeifer Guojian Zhang Dehai Li | 2021 | Synthetic and Systems Biotechnology2021，6，2： | 0 |
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