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| 1 | Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes显示文摘AIM To study the cell types,localization,distribution density and morphology of APUDcells in the intestinal mucosa of stomachlessteleost fishes.METHOD By using the peroxidase-antiperoxidase complex(PAP)immunocytochemical staining technique theidentification,localization and morphology ofimmunoreactive(IR)endocrine cells seattered inthe intestinal mucosa of grass carp(Cyenopharyngodon idellus),black carp(Mylopharyngodon piceus)and common carp(Cyprinus carpio)were investigated with 20kinds of antisera prepared against mammalianpeptide hormones of APUD cells,and likewise byusing avidin-biotin-peroxidase complex(ABC)method those of silver carp(Hypophthalmichthys molitrix),bighead(Aristichthys nobilis),silver crucian carp(Carassius gibelio)and bluntnose black bream(Megalobrama amblyocephala)were alsostudied with 5 different antisera.Thereplacement of the first antiserum by phosphatebuffered saline(PBS)was employed as a control.IR endocrine cells were counted with asquare-mesh ocular micrometer from 10 fieldsselected randomly in every section of each partof the intestine specimen.The average numberof IR endocrine cells per mm2 was counted toquantify their distribution density.RESULT Gastrin(GAS)-,Gastric inhibitorypeptide(GIP)-,glucagon(GLU)-,glucagon-likeimmunoreactants(GLI)-,bovine pancreaticpolypeptide(BPP)-,leucine-enkephalin(ENK)-and substance P(SP)-IR endocrine cells werefound in the gut of grass carp,black carp andcommon carp,and somatostatin(SOM)-IRendocrine cells were only seen in common carp.GAS-,GIP-and GLU-IR endocrine cells werefound in the intestinal mucosa of silver carp,bighead,silver crucian carp and bluntnose blackbream.Most of IR endocrine cells had the higherdistribution density in the foregut and midgut,and were longer in shape.They had a long apicalcytoplasmic process extended to the gut lumenand a basal process extended to adjacent cellsor basement membrane and touched with it.Sometimes,the basal cytoplasmic processformed an enlarged synapse-like structure in thecontiguous part with basement membrane.Thisphenomenon provided new morphologicalevidence for neuroendocrine and paracrinesecretory function of these enteroendocrinecells.CONCLUTION At least 8 kinds of IR endocrinecells were found in the gut of stomachlessteleost species for the first time in China.TheseIR endocrine cells scattering in the gut mucosabelong to the APUD system.Among them,thehormones secreted by SP-,ENK-,SOM-and GLU-IR endocrine cells belong to the peptides of dualdistribution in the brain and gut.This providednew evidence for the concept of brain-gutpeptide.According to the cell types,distribution density,morphologicalcharacteristics and variety in shape of APUDcells in the gut of stomachless teleost fishes,itis deemed that the digestive tract of fishes isalso an endocrine organ of great importance andcomplexity. | Pan QS Fang ZP Zhao YX | 2000 | World Journal of Gastroenterology2000，6，1： | 25 |
| 2 | Extracorporeal shock-wave versus pneumatic ureteroscopic lithotripsy in treatment of lower ureteral calculi显示文摘Aim:To compae the efficacy and complications of extracorporeal shock-wave lithotripsy (SWL) and pneumatic ureteroscopic lithotripsy(URS) in the treatment of lower ureteral calculi.Methods:From August 1997 to June 1999,210 patients with calculi in the distal third of the ureter were treated with SWL and other 180 with URS.The stones were fragmented with either HB-ESWL-V lithotripter or JML-93 pneumatic lithotripter through Wolf 7.5-9.0 Fr ureteroscope.The outcome was assessed in terms of stone clearance rate,re-treatment rate and complication incidence.Results:the stone clearance rate was 78.1% with SWL and 93.3% with URS (p<0.05).SWL had a re-treatment rate of 11.9%,vs 2.2% in the URS group (P<0.05).URS caused ureteral perforation in 3.3% of patients,while it was o with SWL (P<0.05).The differences in the incidence of other complications such as infection and stricture between the two groups were insignificant.conclusion:Though the selection of these two options depends on equipments available and the expertise of the operator,we recomment URS as the optimal treatment for distal ureteral calculi. | Zeng, GQ Zhong, WD Cai, YB Dai, QS Hu, JB Wei, HA | 2002 | Asian Journal of Andrology2002，4，4： | 17 |
| 3 | A novel-designed Escherichia coli for the production of various polyhydroxyalkanoates from inexpensive substrate mixture显示文摘 | Li, R Chen, Q Wang, PG Qi, QS | 2007 | 中国生物学文摘2007，21，10： | 13 |
| 4 | Cloning and expression of core gene cDNA of Chinese hepatitis C virus in cosmid pTM3显示文摘AIM To clone core gene cDNA of Chinesehepatitis C virus(HCV)into eukaryoticexpression vector cosmid pTM3 and to expressHCV core antigen in HepG2 cells.METHODS Core gene cDNA of HCV wasintroduced into eukaryotic expression vectorcosmid pTM3.Using vaccinia virus/bacteriophage T7 hybrid expression system,HepG2 cells were transfected with therecombinant plasmid pTM3-Q534 by lipofectin.RESULTS From the transfected bacteriaTop10F’,2 pTM3-Q534 clones containing therecombinant plasmid were identified fromrandomly selected 10 ampicillin-resistantcolonies.By reverse transcription PCR andindirect immunofluorescence technique,HCVRNA and core protein was identified in HepG2cells transfected with the recombinant plasmid.CONCLUSION The construction of arecombinant plasmid and the expression of coregene cDNA of HCV in HepG2 was successful. | Jiang RL Lu QS Luo KX | 2000 | World Journal of Gastroenterology2000，6，2： | 12 |
| 5 | The production of polyhydroxyalkanoates in recombinant Escherichia coli显示文摘 | Li, R Zhang, HX Qi, QS | 2007 | 中国生物学文摘2007，21，10： | 8 |
| 6 | Gravity field and crustal structure of Gyirong-Lugu Region in western Xizang (Tibet) Plateau显示文摘THE Gyirong-Lugu gravity profile in western Xizang Plateau was carried out by Institute ofGeophysics,Chinese Academy of Sciences in 1994—1995.The data of this gravity profile fillthe gaps in the research of gravity field of western Xizang Plateau and provide basic informa-tion for the study of the whole structure and dynamical mechanism of Xizang Plateau. | Wang, QS Wu, CZ Jiang, WW | 1997 | Chinese Science Bulletin1997，42，8： | 3 |
| 7 | Simultaneous multi-tear exclusion:an optimal strategy for type B thoracic aortic dissection initially proved by a single center's 8 years experience显示文摘背景 Endovascular 接枝stent 广泛地被用来对待胸的大动脉的 dissection.However ,很少信息都不关于跟随八年用为斯坦福的治疗的同时的多眼泪排除的经验打 B 胸的大动脉的解剖导致到1月2006,29日类型 B 胸的大动脉的解剖病人的从1998年9月的没有不利 events.Methods 的成功的大动脉的改变的多重 tears.This 报告细节的同时的排除的结果是可得到的( 24 个人, 5 | YUAN Liang-xi BAO Jun-min ZHAO Zhi-qing FENG Xiang OU Le-feng FENG Rui LU Qing-sheng MEI Zhi-jun JING Zai-ping Department of Vascular Surgery,Changhai Hospital,Second Military Medical University Institute of Vascular Surgery of Chinese People’s Liberation Army Clinic Center of Vascular Diseases of Shanghai National Key Department of Vascular Surgery of China Shanghai 200433,China (Yuan LX,Bao JM,Zhao ZQ,Feng X,Ou LF,Feng R,Lu QS,Mei ZJ and Jing ZP) | 2007 | Chinese Medical Journal2007，，24： | 3 |
| 8 | Collagen content measure of normal people's cartilage of joint 显示文摘 | Zhu QS Yang YG | 1993 | Chin Med J1993，73，3： | 2 |
| 9 | Self-limiting nature of seasonal cholera epidemics:role of host-mediated amplification of phage显示文摘 | Faruque SM Islam MJ Ahmad QS | 2005 | Proc Natl Acad Sci USA2005，102，17： | 1 |
| 10 | Silibinin activated p53 and induced autophagic death in human fibrosarcoma HT1080 cells via reactive oxygen species-p38 and c-Jun N-terminal kinase path-ways 显示文摘 | Duan WJ Li QS Xia MY | 2011 | Biol Pharm Bull2011，34，1： | 1 |
| 11 | Prevention and treatment of ocular inflammation with a new class of non-steroidal anti-inflammatory agents显示文摘 | Chiou GCY Yao QS Chang MS | 1994 | J Ocular Pharmacol1994，10，： | 1 |
| 12 | Ascorbic acid induces redifferentiation and growth inhibition in human hepatoma cells by increasing endogenous hydrogen peroxide显示文摘 | Zheng QS Zhang YT Zheng RL | 2002 | Pharmazie2002，57，11： | 1 |
| 13 | Cloning and expression of a novel tinoblastoma binding protein cDNA,RBBP10显示文摘 | Chen JZ Yang QS Wang S | 2002 | Biochem Genet2002，40，78： | 1 |
| 14 | Full-scalemeasurements and numerical evaluation of wind-induced vibration of a 63-storyreinforced concrete tall building 显示文摘 | LI QS WUJ R LIANGSG etal | 2004 | Engineering Structures2004，26，12： | 1 |
| 15 | Antibacterial activityand mechanism of Silver nanoparticles onEscherichia coli显示文摘 | Li WR Xie XB Shi QS | 2010 | Applied Microbiology andBiotechnology2010，85，4： | 1 |
| 16 | Hand-assisted laparoscopic ver-sus open right hemicolectomy: Short-term outcomes in a single in-stitution from China显示文摘 | Sheng QS Lin JJ Chen WB | 2012 | Surg Laparosc Endosc Percutan Tech2012，22，3： | 1 |
| 17 | Papillary thyroid carcinoma on sonography显示文摘 | Li QS Chen SH Xiong HH | 2010 | Clin Imaging2010，34，2： | 1 |
| 18 | Genistein and its synthetic analogs as anticancer agents显示文摘 | Li Qs Li CY Li ZL | 2012 | A nti-cancer agents in medicinal chemistry2012，12，3： | 1 |
| 19 | Pedicle screw insertion accuracy with different assisted methods: a systematic review and meta-anal- ysis of comparative studies 显示文摘 | Tian NF Huang QS Zhou P | 2011 | Eur Spine J2011，20，6： | 1 |
| 20 | Aluminum-induced apoptosis in cultured cortical neurons and its effect on SAPK/JNK signal transduction pathway显示文摘 | Fu HI Hu QS Lin ZN | 2003 | Brain Res2003，980，1： | 1 |
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