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| 1 | 长春市儿童医院1998~2001年婴幼儿杯状病毒腹泻流行病学研究显示文摘人类杯状病毒(humancalicivirus,HuCV)是引起儿童和成人非菌性胃肠炎的主要病原之一。为了掌握HuCV在我国的流行情况,1998年7月至2001年6月,从长春市儿童医院2343例5岁以下腹泻患儿中共收集粪便标本1264份,其中1056份来自2135例住院患儿。对轮状病毒检测为阴性的588份标本,经多价酶免疫试验(EIA)和两组引物反转录-聚合酶链反应(RT-PCR)检测HuCV,202份为阳性,其中住院患儿标本178份,HuCV检出率为16 9%。HuCV腹泻以2岁以下儿童为主(占96%),流行高峰季节为11月至次年3月。选择17株HuCV进行分子鉴定,15株属GⅡ 4群,1株属GⅡ 3群,另1株属GⅠ 2群,表明GⅡ 4群HuCV是我国流行的优势株。根据HuCV住院患儿的监测资料初步估计,HuCV腹泻住院率约为0 5‰~2 4‰。讨论了长春地区HuCV的流行趋势和疾病负担。以上结果为我国HuCV腹泻的预防和控制提供了科学依据。 | 谢华萍 方肇寅 王光 孙利炜 吕红霞 Zhong WM Tai JH 林思恩 童志礼 章青 Glass RI Jiang X | 2002 | 病毒学报2002，18，4： | 75 |
| 2 | 微小RNA-1和微小RNA-29b对急性心肌梗死后不良心室重塑的诊断和预后价值显示文摘已知微小RNA(microRNAs,miRs)在急性心肌梗死(acute myocardial infarction,AMI)临床前期模型中可发挥纤维化和抗纤维化作用。为了评估miR-1,miR-21,miR-29b和miR-92a能否作为患者发生AMI后不良心室重塑(adverse ventricular remodeling,AVR)的循环生物标志物, | 刘莉 叶鹏 Grabmaier U Clauss S Gross L Klier I Franz WM Steinbeck G Wakili R Theiss HD Brenner C | 2017 | 中华高血压杂志2017，25，7： | 17 |
| 3 | Sm-Nd isotopic age and genesis of gabbro xenoliths in Daoxian County, Hunan Province显示文摘MANY geologists pay attention to Mesozoic tectonomagmatic activity and the associating multi-metal ore deposits in South China. However, the age of the transformatiori of tectonic regimein the area is little studied. Occurrence of gabbro xenoliths in Mesozoic alkaline basalts inDaoxian region, Hunan Province, shows that the lithosphere in the region was intensely ex-tended and thinned during the Mesozoic, followed by underplating of mantle-derived mag- | Guo, F Fan, WM Lin, G Lin, YX | 1997 | Chinese Science Bulletin1997，42，21： | 14 |
| 4 | Dynamic tracking of stem cells in an acute liver failure model显示文摘AIM:To investigate a dual labeling technique,which would enable real-time monitoring of transplanted embryonic stem cell(ESC) kinetics,as well as long-term tracking.METHODS:Liver damage was induced in C57/BL6 male mice(n = 40) by acetaminophen(APAP) 300 mg/kg administered intraperitoneally.Green fluorescence protein(GFP) positive C57/BL6 mouse ESCs were stained with the near-infrared fluorescent lipophilic tracer 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide(DiR) immediately before transplantationinto the spleen.Each of the animals in the cell therapy group(n = 20) received 5 × 10 6 ESCs 4 h following treatment with APAP.The control group(n = 20) received the vehicle only.The distribution and dynamics of the cells were monitored in real-time with the IVIS Lumina-2 at 30 min post transplantation,then at 3,12,24,48 and 72 h,and after one and 2 wk.Immunohistochemical examination of liver tissue was used to identify expression of GFP and albumin.Plasma alanine aminotransferase(ALT) was measured as an indication of liver damage.RESULTS:DiR-stained ESCs were easily tracked with the IVIS using the indocyanine green filter due to its high background passband with minimal background autofluorescence.The transplanted cells were confined inside the spleen at 30 min post-transplantation,gradually moved into the splenic vein,and were detectable in parts of the liver at the 3 h time-point.Within 24 h of transplantation,homing of almost 90% of cells was confirmed in the liver.On day three,however,the DiR signal started to fade out,and ex vivo IVIS imaging of different organs allowed signal detection at time-points when the signal could not be detected by in vivo imaging,and confirmed that the highest photon emission was in the liver(P < 0.0001).At 2 wk,the DiRsignal was no longer detectable in vivo ;however,immunohistochemistry analysis of constitutively-expressed GFP was used to provide an insight into the distribution of the cells.GFP +ve cells were detected in tissue sections resembling hepatocytes and were dispersed throughout the hepatic parenchyma,with the presence of a larger number of GFP +ve cells incorporated within the sinusoidal endothelial lining.Very faint albumin expression was detected in the transplanted GFP +ve cells at 72 h;however at 2 wk,few cells that were positive for GFP were also strongly positive for albumin.There was a significant improvement in serum levels of ALT,albumin and bilirubin in both groups at 2 wk when compared with the 72 h time-point.In the cell therapy group,serum ALT was significantly(P = 0.016) lower and albumin(P = 0.009) was significantly higher when compared with the control group at the 2 wk time-point;however there was no difference in mortality between the two groups.CONCLUSION:Dual labeling is an easy to use and cheap method for longitudinal monitoring of distribution,survival and engraftment of transplanted cells,and could be used for cell therapy models. | Tarek Ezzat Dipok Kumar Dhar Massimo Malago Steven WM Olde Damink | 2012 | World Journal of Gastroenterology2012，18，6： | 12 |
| 5 | 慢慢型房室结折返性心动过速的电生理机制和射频导管消融治疗显示文摘目的探讨慢慢型房室结折返性心动过速(AVNRT)的电生理机制和不同射频导管消融方法的治疗效果.方法 812例AVNRT患者分为两组,第1组500例,比较慢慢型中前传慢径和逆传慢径的成功消融部位的异同、比较在慢慢型和快慢型中选择性地消融逆传慢径部位的异同.第2组312例,在设想慢慢型AVNRT折返机制的基础上,前瞻性地对慢慢型仅选择性消融前传慢径而不消融逆传慢径.结果第1组59例慢慢型AVNRT的前传和逆传慢径的传导时间和成功消融的部位明显不同,逆传慢径多在冠状静脉窦(CS)窦口内或CS近端消融成功,而前传慢径多在三尖瓣环和CS窦口之间消融成功;慢慢型与快慢型的逆传慢径有明显不同的传导时间、递减特性和解剖分布.在第2组前瞻性地仅消融前传慢径治疗22例慢慢型组中,在三尖瓣环和CS窦口之间成功消融前传慢径并治愈AVNRT后,21例逆传慢径功能不变,其逆传慢径最早心房插入点部位与前传慢径消融部位不同.所有812例AVNRT均消融成功,第1组在3年以上的随访中,387例慢快型复发1例(0.3%),59例慢慢型复发6例(10%),54例快慢型无复发.第2组312例3~48(23±12)个月的随访中,慢快型复发2例(0.5%),慢慢型和快慢型无复发.结论 (1)慢慢型AVNRT应用电生理特性和解剖分布不同的两条慢径形成折返环的前传支和逆传支,其前传和逆传慢径的解剖基础可能分别为房室结的右侧后延伸和左侧后延伸;(2)在大多数慢慢型AVNRT中,仅消融前传慢径有很高的成功率和低复发率. | 王祖禄 Jackman WM 韩雅玲 梁延春 梁明 徐凯 | 2005 | 中华心律失常学杂志2005，9，1： | 10 |
| 6 | Role of sonic hedgehog in maintaining a pool of proliferating stem cells in the human fetal epidermis显示文摘 | Zhou JX Jia LW Liu WM Miao CL Liu S Cao YJ Duan EK | 2006 | 中国生物学文摘2006，20，5： | 8 |
| 7 | Awareness of and attitudes towards infertility and its treatment: a cross-sectional survey of men in a United States primary care population显示文摘以前的研究在不孕的治疗描述了种族、社会经济的不同。象态度和了解那样的耐心的因素可能正在贡献因素。因为主要照顾经常是用作一个条目进药的另外的区域的背景,我们寻求了在主要照顾背景评估人男不孕的态度和了解。做这,我们从在亚特兰大等待房间的二个主要照顾诊所在 210 个人执行了人对人的健康问题的态度的代表性的调查,乔治娅。调查与 closed-ended 问题项目是自我管理的并且是在长度的约 20 min。走近的 310 个人, 210 同意参与并且归还了完成的调查。总的来说, 52% 人说他们是 “ very”或 “ somewhat”熟悉不孕并且 25% 为不孕熟悉了治疗。一些人为男不孕作为治疗听说了外科(21%) 和药(35%) 。有条件的了解和熟悉在高社会经济的地位人是更大的(即有收入 > 的学院毕业生或那些; $100 k 每年) 但是没在 multivariate 分析上由种族不同。对不孕的态度与是更可能的显示不孕是一个严重条件,并且相信它减少的非白种人人一起由种族变化了,到担心不孕一个人的 quality-of-life。因此,了解的缺乏,然而并非否定态度,可以在不孕的治疗贡献以前描述的不同。 | Robert S Gerhard Chad WM Ritenour Michael Goodman Dipak Vashi Wayland Hsiao | 2014 | Asian Journal of Andrology2014，16，6： | 7 |
| 8 | New Bibenzyl and Phenanthrenedione from Dendrobium densiflorum显示文摘A new bibenzyl 1 and a new phenanthrenedione 2 were identified from the stems of Dendrobium densiflorum (Orchidaceae). | Fan, CQ Zhao, WM Qin, GW | 2000 | Chinese Chemical Letters2000，11，8： | 6 |
| 9 | Overexpression/amplification of HER-2/neu is uncommon in hepatocellular carcinoma显示文摘 | Xian ZH Zhang SH Cong WM Wu WQ Wu MC | 2005 | 第二军医大学学报2005，26，11： | 6 |
| 10 | Expression of aspartyl beta-hydroxylase and its clinicopathological significance in hepatocellular carcinoma显示文摘 | Xian ZH Zhang SH Cong WM Yan HX Wang K Wu MC | 2006 | 第二军医大学学报2006，27，5： | 6 |
| 11 | Colonoscopic yield of colorectal neoplasia in daily clinical practice显示文摘AIM:To assess the prevalence and location of ad-vanced neoplasia in patients undergoing colonoscopy,and to compare the yield per indication.METHODS:In a multicenter colonoscopy survey (n = 18 hospitals) in the Amsterdam area (Northern Holland),data of all colonoscopies performed during a three month period in 2005 were analyzed. The location and the histological features of all colonic neoplasia were recorded. The prevalence and the distribution ofadvanced colorectal neoplasia and differences in yield between indication clusters were evaluated. Advanced neoplasm was defi ned as adenoma > 10 mm in size,with > 25% villous features or with high-grade dyspla-sia or cancer.RESULTS:A total of 4623 eligible patients underwent a total colonoscopy. The prevalence of advanced neo-plasia was 13%,with 281 (6%) adenocarcinomas and 342 (7%) advanced adenomas. Sixty-seven percent and 33% of advanced neoplasia were located in the distal and proximal colon,respectively. Of all patients with right-sided advanced neoplasia (n = 228),51% had a normal distal colon,whereas 27% had a syn-chronous distal adenoma. Ten percent of all colono-scopies were performed in asymptomatic patients,7% of whom had advanced neoplasia. In the respective procedure indication clusters,the prevalence of right-sided advanced neoplasia ranged from 11%-57%. CONCLUSION:One out of every 7-8 colonoscopies yielded an advanced colorectal neoplasm. Colonoscopy is warranted for the evaluation of both symptomatic and asymptomatic patients. | Jochim S Terhaar sive Droste Mike E Craanen Rene WM van der Hulst Joep F Bartelsman Dick P Bezemer Kim R Cappendijk Gerrit A Meijer Linde M Morsink Pleun Snel Hans ARE Tuynman Roy LJ van Wanrooy Eric IC Wesdorp Chris JJ Mulder | 2009 | World Journal of Gastroenterology2009，15，9： | 6 |
| 12 | Emerging radiotherapy technologies and trends in nasopharyngeal cancer显示文摘AbTechnology has always driven advances in radiotherapy treatment.In this review,we describe the main technological advances in radiotherapy over the past decades for the treatment of nasopharyngeal cancer(NPC)and highlight some of the pressing issues and challenges that remain.We aim to identify emerging trends in radiation medicine.These include advances in personalized medicine and advanced imaging modalities,standardization of planning and delineation,assessment of treatment response and adaptive re-planning,impact of particle therapy,and role of artificial intelligence or automation in clinical care.In conclusion,we expect significant improvement in the therapeutic ratio of radiotherapy treatment for NPC over the next decade. | Michelle Tseng Francis Ho Yiat Horng Leong Lea ChoungWong Ivan WK Tham Timothy Cheo Anne WM Lee | 2020 | Cancer Communications2020，40，9： | 5 |
| 13 | 从慢慢型和慢快型房室结折返性心动过速电生理特性的差异分析折返环的不同显示文摘目的从慢慢型房室结折返性心动过速(AVNRT)和慢快型AVNRT的电生理特性的差异分析两型AVNRT间折返环的不同。方法在500例AVNRT患者中的59例慢慢型和60例慢快型之间,比较部分电生理特性的异同;同时在部分慢慢型和慢快型患者中应用2种方法(1)比较起搏时和心动过速时的HA间期的长度;(2)比较心动过速时心室刺激重整心动过速的不同。比较下传共径(LCP)的异同。结果慢慢型的前传慢径和逆传慢径有明显不同的传导时间;慢慢型的逆传慢径与慢快型的逆传快径有明显不同的传导时间和递减特性;和慢快型相比,2种方法均显示慢慢型有较长的LCP。结论(1)慢慢型AVNRT中前传慢径和逆传慢径的传导时间明显不同;慢慢型较慢快型有较长的下传共径;(2)研究结果支持慢慢型AVNRT可能应用房室结的右侧后延伸和左侧后延伸分别形成心动过速的前传和逆传支而形成折返。 | 王祖禄 Jackman WM | 2005 | 中华心律失常学杂志2005，9，4： | 5 |
| 14 | Expression of matrix metallo-proteinase-28 in human normal cytotrophoblast cells and a choriocarcinoma cell line,JEG-3显示文摘There are striking similiarities present between the behavior of invasive placentaL cells and that of invasive cancer cells. Matrix metalloproteinases (MMPs) are one of the most important mediators. MMP-28, the new member of MMPs, was sequenced and identified recently. Expression of MMP-28 mRNA and protein in the cytotrophoblast cells and a choriocarcinoma cell line, JEG-3 cell, was conducted by zymography, RT-PCR and Northern blot. There is MMP-28 mRNA expression in both the cytotrophoblast cells and JEG-3 cells by RT-PCR. The activity of MMP-28 in cytotrophoblast cells was significantly weaker than that in JEG-3 (P < 0.01) by zymography. Furthermore, mRNA expression of MMP-28 was significantly stronger (P < 0.001) in JEG-3 than in human cytotrophoblast cells in a time-dependent way by Northern Blot. Our results suggest that MMP-28 may play a role in some of the tissue-remodeling events associated with normal pregnancy and tumor progression. | Zhang, J Cao, YJ Liu, WM Zhao, BS Zeng, GQ Duan, EK | 2002 | Chinese Science Bulletin2002，47，9： | 4 |
| 15 | FTO基因单核苷酸多态性对肥胖运动减重效果的影响存在性别差异显示文摘携带肥胖易感基因的个体在发生肥胖后,运动减重的效果是否仍然受基因多态性影响,在国内外为数不多的研究中存在较大争议,在客观上也需要针对不同人种和不同民族开展研究。解放军总医院第二医学中心健康管理研究院联合北京博奥晶典生物技术有限公司等机构的科研团队,首次发现在中国汉族成年肥胖人群中,不同FTO基因型的运动减重效果存在差异,尤其在男性肥胖者中这种差异性更为显著。该研究自2018年至2019年,在解放军总医院第二医学中心健康管理研究院医学减重门诊招募成年肥胖者240例(男女各120例,28≤BMI≤40),利用其与中国科学院合肥物质科学研究院联合开发的慢性病运动康复系统,对受试者开展12周精准化中等强度的有氧运动干预,精准控制运动强度和运动量,对完成者进行FTO基因(rs8050136)分型检测,分析运动干预前后肥胖相关指标的变化程度与FTO基因单核苷酸多态性的相关性。研究发现,携带FTO基因的中国汉族成年肥胖者可以通过运动实现减重,且在男性肥胖者中,携带FTO基因者比未携带者的体重和体质指数下降程度更为显著。该发现提示,想要获得更佳的运动减重效果,可根据基因检测结果制定个体化运动减肥方案,对于经基因检测判断为运动减重速度偏慢的肥胖者,可考虑突破中等强度有氧运动的局限性,通过运动要素的新组合来提高减重速度。 | 汪菲(编译) Wang WM Yang K Wang SJ | 2022 | 中华健康管理学杂志2022，16，3： | 3 |
| 16 | Mitochondrial neuronal uncoupling proteins:a target for potential disease-modification in Parkinson’s disease Philip显示文摘This review gives a brief insight into the role of mitochondrial dysfunction and oxidative stress in the converging pathogenic processes involved in Parkinson’s disease(PD).Mitochondria provide cellular energy in the form of ATP via oxidative phosphorylation,but as an integral part of this process,superoxides and other reactive oxygen species are also produced.Excessive free radical production contributes to oxidative stress.Cells have evolved to handle such stress via various endogenous anti-oxidant proteins.One such family of proteins is the mitochondrial uncoupling proteins(UCPs),which are anion carriers located in the mitochondrial inner membrane.There are five known homologues(UCP1 to 5),of which UCP4 and 5 are predominantly expressed in neural cells.In a series of previous publications,we have shown how these neuronal UCPs respond to 1-methyl-4-phenylpyridinium(MPP+;toxic metabolite of MPTP)and dopamine-induced toxicity to alleviate neuronal cell death by preserving ATP levels and mitochondrial membrane potential,and reducing oxidative stress.We also showed how their expression can be influenced by nuclear factor kappa-B(NF-B)signaling pathway specifically in UCP4.Furthermore,we previously reported an interesting link between PD and metabolic processes through the protective effects of leptin(hormone produced by adipocytes)acting via UCP2 against MPP+-induced toxicity.There is increasing evidence that these endogenous neuronal UCPs can play a vital role to protect neurons against various pathogenic stresses including those associated with PD.Their expression,which can be induced,may well be a potential therapeutic target for various drugs to alleviate the harmful effects of pathogenic processes in PD and hence modify the progression of this disease. | WL Ho Jessica WM Ho Hui-Fang Liu Danny HF So Zero HM Tse Koon-Ho Chan David B Ramsden Shu-Leong Ho | 2012 | Translational Neurodegeneration2012，1，1： | 3 |
| 17 | Grayanane Diterpenoids from Pieris formosa显示文摘Three grayanane diterpenoids 1-3 were isolated from Pierisfonnosa. 1 was identifiedas a new natural product and 2 and 3 as known grayanoside C and grayanotoxin XVIII on the basisof spectral analysis. | Wang, LQ Ding, BY Zhao, WM Qin, GW | 1998 | Chinese Chemical Letters1998，9，5： | 3 |
| 18 | Effects of mesenchymal stem cells on differentiation, maturation, and function of human monocyte-derived dendritic cells显示文摘 | Zhang,W Ge,W Li,CH You,SG Liao,LM Han,Q Deng,WM Zhao,RCH | 2005 | 中国生物学文摘2005，19，3： | 3 |
| 19 | Trends in treatment and overall survival among patients with proximal esophageal cancer显示文摘BACKGROUND The management of proximal esophageal cancer differs from that of tumors located in the mid and lower part of the esophagus due to the close vicinity of vital structures.Non-surgical treatment options like radiotherapy and definitive chemoradiation(CRT)have been implemented.The trends in(non-)surgical treatment and its impact on overall survival(OS)in patients with proximal esophageal cancer are unclear,related to its rare disease status.To optimize treatment strategies and counseling of patients with proximal esophageal cancer,it is therefore essential to gain more insight through real-life studies.AIM To establish trends in treatment and OS in patients with proximal esophageal cancer.METHODS In this population-based study,patients with proximal esophageal cancer diagnosed between 1989 and 2014 were identified in the Netherlands Cancer Registry.The proximal esophagus consists of the cervical esophagus and the upper thoracic section,extending to 24 cm from the incisors.Trends in radiotherapy,chemotherapy,and surgery,and OS were assessed.Analyses were stratified by presence of distant metastasis.Multivariable Cox proportional hazards regression analyses was performed to assess the effect of period of diagnosis on OS,adjusted for patient,tumor,and treatment characteristics.RESULTS In total,2783 patients were included.Over the study period,the use of radiotherapy,resection,and CRT in non-metastatic disease changed from 53%,23%,and 1%in 1989-1994 to 21%,9%,and 49%in 2010-2014,respectively.In metastatic disease,the use of chemotherapy and radiotherapy increased over time.Median OS of the total population increased from 7.3 mo[95%confidence interval(CI):6.4-8.1]in 1989-1994 to 9.5 mo(95%CI:8.1-10.8)in 2010-2014(logrank P<0.001).In non-metastatic disease,5-year OS rates improved from 5%(95%CI:3%-7%)in 1989-1994 to 13%(95%CI:9%-17%)in 2010-2014(logrank P<0.001).Multivariable regression analysis demonstrated a significant treatment effect over time on survival.In metastatic disease,median OS was 3.8 mo(95%CI:2.5-5.1)in 1989-1994,and 5.1 mo(95%CI:4.3-5.9)in 2010-2014(logrank P=0.26).CONCLUSION OS significantly improved in non-metastatic proximal esophageal cancer,likely to be associated with an increased use of CRT.Patterns in metastatic disease did not change significantly over time. | Judith de Vos-Geelen Sandra ME Geurts Liselot BJ Valkenburg-van Iersel Evelien JM de Jong Vivianne CG Tjan-Heijnen Margreet van Putten Valery EPP Lemmens Heike I Grabsch Nadia Haj Mohammad Frank JP Hoebers Chantal V Hoge Paul M Jeene Hanneke WM van Laarhoven Tom Rozema Marije Slingerland Grard AP Nieuwenhuijzen | 2019 | World Journal of Gastroenterology2019，25，47： | 2 |
| 20 | ATG16L1 and NOD2 polymorphisms enhance phagocytosis in monocytes of Crohn's disease patients显示文摘AIM:To investigate if the presence of relevant genetic polymorphisms has effect on the effectual clearance of bacteria by monocytes and granulocytes in patients with Crohn’s disease(CD).METHODS:In this study,we assessed the differential responses in phagocytosis by measuring the phagocytic activity and the percentage of active phagocytic monocytes and granulocytes in inflammatory bowel disease patients as well as healthy controls.As both autophagy related like 1(ATG16L1)and immunityrelated guanosine triphosphatase gene are autophagy genes associated with CD and more recently nucleo-tide-binding ligomerization domain-containing protein2(NOD2)has been identified as a potent inducer of autophagy we genotyped the patients for these variants and correlated this to the phagocytic reaction.The genotyping was done with restriction fragment length polymorphisms analysis and the phagocytosis was determined with the pHrodo?Escherichia coli Bioparticles Phagocytosis kit for flowcytometry.RESULTS:In this study,we demonstrate that analysis of the monocyte and granulocyte populations of patients with CD and ulcerative colitis showed a comparable phagocytic activity(ratio of mean fluorescence intensity)between the patient groups and the healthy controls.CD patients show a significantly higher phagocytic capacity(ratio mean percentage of phagocytic cells)compared to healthy controls(51.91%±2.85%vs 37.67%±7.06%,P=0.05).The extend of disease was not of influence.However,variants of ATG16L1(WT:2.03±0.19 vs homozygoot variant:4.38±0.37,P<0.009)as well as NOD2(C-ins)(heterozygous variant:42.08±2.94 vs homozygous variant:75.58±4.34(P=0.05)are associated with the phagocytic activity in patients with CD.CONCLUSION:Monocytes of CD patients show enhanced phagocytosis associated with the presence of ATG16L1 and NOD2 variants.This could be part of the pathophysiological mechanism resulting in the disease. | Simone CS Wolfkamp Caroline Verseyden Esther WM Vogels Sander Meisner Kirsten Boonstra Charlotte P Peters Pieter CF Stokkers Anje A te Velde | 2014 | World Journal of Gastroenterology2014，20，10： | 2 |
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