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| 1 | Risk assessment of heavy metals in soils and vegetables around nonferrous metals mining and smelting sites,Baiyin,China显示文摘A field survey was conducted to investigate the metal and arsenic contamination in soils and vegetables on four villages (Shuichuan (SCH), Beiwan (BWA), Dongwan (DWA) and Wufe (WFE)) located along, Baiyin, China, and to evaluate the possible health risks to local population through foodchain. Results show that the most significantly contaminated soils occurred upstream at SCH where Cd, Cu and As concentrations exceeded maximum allowable concentrations for Chinese agricultural soil. Further downstream the degree of contamination semi-systematically decreased in concentrations of metal. Generally, the leafy vegetables were more heavily contaminated than non-leafy vegetables. Chinese cabbage is the most severely contaminated, the concentrations of Cd exceeded the maximum permit levels (0.05 mg/kg) by 4.5 times. Bio-accumulate factor also shows that an entry of Cd to food chain plants is the greatest potential. Furthermore, the estimated daily intake amounts of the considered toxic elements (Cd, Pb and Cu) from the vegetables grown at SCH and BWA and DWA have exceeded the recommended dietary allowance levels. Thus, the vegetables grown in three villages above, which affected by Baiyin mining and smelting have a health hazard for human consumption. | LI Yu WANG Yan-bin GOU Xin SU Yi-bing WANG Gang | 2006 | Journal of Environmental Sciences2006，18，6： | 84 |
| 2 | Application of the CRISPR-Cas System for Efficient Genome Engineering in Plants显示文摘 | Mao, Yanfei Zhang, Hui Xu, Nanfei Zhang, Botao Gou, Feng Zhu, Jian-Kang | 2013 | Molecular Plant2013，6，6： | 132 |
| 3 | Diurnal freeze/thaw cycles of the ground surface on the Tibetan Plateau显示文摘The exchange of energy and water between the lithosphere and atmosphere mainly takes place at the ground surface. Therefore, freeze/thaw condition at the ground surface is an important factor in ex- amining the interactions between the land surface and atmosphere. Based on the observation data obtained by CEOP/CAMP-Tibet, the diurnal freeze/thaw cycles of the ground surface near Naqu, central Tibetan Plateau was preliminarily analyzed. The results show that the surface layer was completely frozen for approximately one month. However, the time that the ground surface experienced diurnal freeze/thaw cycles was about 6 months. The high frequency of freeze/thaw cycles at the ground surface significantly influences water and energy exchanges between ground and atmosphere over half a year. The interaction processes between the ground and atmosphere under different soil conditions (such as complete thaw, complete freeze and diurnal freeze/thaw cycles) are issues worthy of further examina- tion. | YANG MeiXue YAO TanDong GOU XiaoHua HIROSE Nozomu FUJII Hide Yuki HAO LiSheng D. F. LEVIA | 2007 | Chinese Science Bulletin2007，52，1： | 44 |
| 4 | Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis显示文摘棉花纤维在延伸的开始以后很快伸长,最后导致大量纤维素的存款。为了在快延伸和第二等的细胞壁合成揭示棉花纤维房间的特征,上演,我们比较了 respectivetranscriptomes 和代谢物侧面。由 cDNA 数组的 transcriptomes 的比较分析识别了是在纤维开发期间调整的差别的 633 基因。用变量把纤维样品划分了成四的表示基因的 Principalcomponent 分析(PCA ) 组织,它发育阶段是诊断的。如果,类似的组织结果也被发现我们是的 usenon 极或极的代谢物为开发纤维的 PCA 的变量。植物生长素发信号,松开墙并且脂类代谢在纤维延伸期间是高度活跃的,而纤维素生合成是占优势的,许多另外的新陈代谢的小径是在第二等的细胞壁合成调整的 down,上演。抄本和代谢物侧面和酶活动在向纤维素合成表明棉花纤维开发的一个专门化过程是一致的。这些数据证明在某个阶段的那个棉花纤维房间有它棉花纤维房间的自己的唯一的特征,和发育阶段能被他们的抄本和代谢物侧面区分。在第二等的细胞壁合成阶段期间,新陈代谢的小径被流进纤维素合成。 | Jin-Ying Gou Ling-Jian Wang Shuang-Ping Chen Wen-Li Hu Xiao-Ya Chen | 2007 | Cell Research2007，17，5： | 48 |
| 5 | Research progress on reduced lodging of high-yield and-density maize显示文摘Increasing plant density is an effective way to enhance maize yield, but often increases lodging rate and severity, significantly elevating the risk and cost of maize production. Therefore, lodging is a major factor restricting future increases in maize yield through high-density planting. This paper reviewed previous research on the relationships between maize lodging rate and plant morphology, mechanical strength of stalks, anatomical and biochemical characteristics of stalks, root characteristics, damage from pests and diseases, environmental factors, and genomic characteristics. The effects of planting density on these factors and explored possible ways to improve lodging resistance were also analyzed in this paper. The results provide a basis for future research on increasing maize lodging resistance under high-density planting conditions and can be used to develop maize cultivation practices and lodging-resistant maize cultivars. | XUE Jun XIE Rui-zhi ZHANG Wang-feng WANG Ke-ru HOU Peng MING Bo GOU Ling LI Shao-kun | 2017 | Journal of Integrative Agriculture2017，16，12： | 42 |
| 6 | Tree ring based streamflow reconstruction for the Upper Yellow River over the past 1234 years显示文摘We established a Juniperus przewalski tree ring width chronology, based on tree ring cores collected from the A’nyêmaqên Mountains. Statistical analysis showed that the chronology was highly correlated with instrumental streamflow records from previous August to current July from the Tangnaihai Station in the upper reaches of the Yellow River, with a correlation coefficient of 0.656. Streamflow for the upper reaches of the Yellow River was reconstructed for the past 1234 years. Low flow periods for the 11-year averaged streamflow reconstruction were definite as lower than mean plus 1 standard deviation, and high flow periods were higher than mean minus 1 standard deviation. Over the past 1234 years, high flows occurred 18 times, and low flows occurred 12 times. The main low flow periods were identified as AD 1140–1156, AD 1295–1309, AD 1473–1500, and AD 1820–1847, and the main high flow periods were identified as AD 846–873, and AD 1375–1400. Extremely low streamflow over the reconstruction period was seen during the late 15th century, coinciding with a widespread drought phenomenon, which took place in the northeastern Tibetan Plateau over the same period. Reconstructed streamflow shows significant low-frequency variability, which is in line with drought variability of neighboring regions, as inferred from tree rings and other proxies. Multi-taper spectral analysis suggests the existence of significant periods of 2–5, 22, 35–38, 55–62, and 114–227 years, particularly significant for cyclic variations of years 159 and 36. | GOU XiaoHua DENG Yang CHEN FaHu YANG MeiXue FANG KeYan GAO LinLin YANG Tao ZHANG Fen | 2010 | Chinese Science Bulletin2010，55，36： | 37 |
| 7 | Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis显示文摘在哺乳动物的精子发生被 piRNA 表示的二个波浪描绘:一个人对应于负责的经典 piRNAs 因为 silencing retrotransponsons 和第二个波浪主要在 pachytene spermatocytes 从 nontransposon intergenic 区域被导出,但是这些 pachytene piRNAs 的功能大部分是未知的。这里,我们在在老鼠伸长指示巨大的 mRNA 消除报导 pachytene piRNAs 的参与 spermatids (ES ) 。我们证明包含鼠科的 PIWI (MIWI ) 和 deadenylase CAF1 的导致 piRNA 的 silencing 建筑群(pi-RISC ) 有选择地在 ES 被装配,它负责经由在体的房间类似于 miRNAs 的行动的机制导致 mRNA deadenylation 和腐烂。如此的一个高度安排的节目看起来充分利用 pachytene piRNAs 的能力从 nontransposon intergenic 区域导出的多样化的指向的庞大的全部剧目。这些调查结果建议 pachytene piRNAs 负责为精子生产作准备,从 ES 使广阔细胞的节目失去活性。 | Lan-Tao Gou Peng Dai Jian-Hua Yang Yuanchao Xue Yun-Ping Hu Yu Zhou Jun-Yan Kang Xin Wang Hairi Li Min-Min Hua Shuang Zhao Si-Da Hu Li-Gang Wu Hui-Juan Shi Yong Li Xiang-Dong Fu Liang-Hu Qu En-Duo Wang Mo-Fang Liu | 2014 | Cell Research2014，24，6： | 39 |
| 8 | MIWI and piRNA-mediated cleavage of messenger RNAs in mouse testes显示文摘piRNA 机械在调停以它的角色著名 transposons 的 epigenetic silencing。最近的研究建议这功能也包含导出 transposon 的抄本的指导 piRNA 的劈开。同样多 piRNAs 也看起来有能力指向多样的 mRNAs,这提起 piRNAs 可以与 siRNAs 比广泛地扮演降级的吸引人的可能性特定的 mRNAs。直接测试这个假设,我们比较了联系的老鼠 PIWI (MIWI ) 有从老鼠睾丸的试验性地识别的劈开的 mRNA 碎片的 piRNAs,和主要发生在的观察劈开地点从 5 ′ 放 10;通常认为的指向结束 piRNAs。我们也注意了强壮的偏爱因为分别地,在核苷酸的 U 和 A 残余在 piRNAs 和 mRNA 碎片放 1 和 10,由“乒乓球”类似于 piRNA 扩大的模式的特征骑车。通过由介绍的 CLIP-seq 和基因表示 MIWI-RNA 相互作用印射,我们发现许多潜在的指向 piRNA 的 mRNAs 直接与 MIWI 交往,表演在 Miwi 的睾丸提高了表达式层次催化变异的鼠标。基于记者的试金进一步揭示了在调停 piRNA 的目标压抑的为 slicer 活动和 MIWI 的装载 piRNA 能力的在 piRNAs 和 mRNA 目标和要求之间的基础配对的重要性。重要地,我们证明某些关键 piRNA 目标的那合适的周转为精子形成是必要的。一起,这些调查结果为男细菌房间开发和成熟在鼠标睾丸和它的中央要求揭示 piRNA 机械的象 siRNA 一样函数。 | Peng Zhang Jun-Yan Kang Lan-Tao GOU Jiajia Wang Yuanchao Xue Geir Skogerboe Peng Dai Da-Wei Huang Runsheng Chen Xiang-Dong Fu Mo-Fang Liu Shunmin He | 2015 | Cell Research2015，25，2： | 35 |
| 9 | Heavy metal contamination and source in arid agricultural soil in central Gansu Province,China显示文摘铜( Cu )的集中,铅( Pb ),铬( Cr ),水银( Hg ),和砷(作为)在在 Daba 村庄, Shajiawuan 村庄, Gangou 村庄和 Sifangwu 村庄里收集的干旱农业、灌溉的农业土壤被测量,在中央 Gansu 省定位了,除了 Hg 和 Pb 的 China.Concentrations 比在在选择干旱农业 soils.Pb 集中的灰石灰性土壤的背景价值由结果显示出的72.46% .These 在中国超过干旱农业土壤的阀值低有有 Pb 的确实严肃的污染,为在 Daba 村庄里的灌溉农业土壤的另外的选择金属的一个细微污染问题。种量分析(PCA ) 被用来估计土壤数据,使用有凯撒正规化的 varimax 旋转。结果证明灌溉因素,农业因素和人为的因素都贡献了在选择化学特性之间的关系。Cu 的累积的主要因素, Pb, Cr, Hg 并且这是石版印刷在干旱农业区域的逻辑因素。有 Cu 的起源的惹人注目的不同, Pb, Cr, Hg 并且作为在在 irrigate 农业和干旱农业之间的农业土壤。 | LI Yu GOU Xin WANG Gang ZHANG Qiang SU Qiong XIAO Guoju | 2008 | Journal of Environmental Sciences2008，20，5： | 27 |
| 10 | Gestational diabetes: weight gain during pregnancy and its relationship to pregnancy outcomes显示文摘Background:Weight gain during pregnancy reflects the mother's nutritional status.However, it may be affected by nutritional therapy and exercise interventions used to control blood sugar in gestational diabetes mellitus (GDM).This study aimed to evaluate weight gain during gestation and pregnancy outcomes among women with GDM.Methods:A retrospective study involving 1523 women with GDM was conducted between July 2013 and July 2016.Demographic data, gestational weight gain (GWG), blood glucose, glycated-hemoglobin level, and maternal and fetal outcomes were extracted from medical records.Relationships between GWG and pregnancy outcomes were investigated using multivariate logistic regression.Results:In total, 451 (29.6%) women showed insufficient GWG and 484 (31.8%) showed excessive GWG.Excessive GWG was independently associated with macrosomia (adjusted odds ratio [aOR] 2.20, 95% confidence interval [CI] 1.50-3.52, P<0.001), large for gestational age (aOR 2.06, 95% CI 1.44-2.93, P<0.001), small for gestational age (aOR 0.49, 95% CI 0.25-0.97, P=0.040), neonatal hypoglycemia (aOR 3.80, 95% CI 1.20-12.00, P=0.023), preterm birth (aOR 0.45, 95% CI 0.21-0.96, P=0.040), and cesarean delivery (aOR 1.45, 95% CI 1.13-1.87, P=0.004).Insufficient GWG increased the incidence of preterm birth (aOR 3.53, 95% CI 1.96-6.37, P<0.001).Conclusions:Both excessive and insufficient weight gain require attention in women with GDM.Nutritional therapy and exercise interventions to control blood glucose should also be used to control reasonable weight gain during pregnancy to decrease adverse pregnancy outcomes. | Bao-Hua Gou Hui-Min Guan Yan-Xia Bi Bing-Jie Ding | 2019 | Chinese Medical Journal2019，，2： | 28 |
| 11 | Circular RNA F-circEA produced from EML4-ALK fusion gene as a novel liquid biopsy biomarker for non-small cell lung cancer显示文摘 | Shuanqyan Tan Qiheng Gou Wenchen Pu Chenglin Guo Yun Yang Ke Wu Yaxin Liu Lunxu Liu Yu-Quan Wei Yong Peng | 2018 | Cell Research2018，28，6： | 24 |
| 12 | A review of third generation SiC fibers and SiC_f/SiC composites显示文摘Compared with the first and second generations SiC fibers, the third generation SiC fibers have obvious improvement in heat-resistance, oxidation-resistance and creep-resistance, which promote the development of SiCf/SiC composite materials. Therefore, the third generation SiC fibers have more advantages and broader prospects in engineering applications. In this paper, the fabrication and properties of the third generation SiC fibers are compared and discussed. The preparation processes of the third generation SiC fibers reinforced SiC matrix composites and their application in aeroengine and nuclear energy fields are summarized, while their future development is prospected as well. | Pengren Wang Fengqi Liu Hao Wang Hao Li Yanzi Gou | 2019 | Journal of Materials Science & Technology2019，35，12： | 24 |
| 13 | Generative Adversarial Networks:Introduction and Outlook显示文摘Recently, generative adversarial networks(GANs)have become a research focus of artificial intelligence. Inspired by two-player zero-sum game, GANs comprise a generator and a discriminator, both trained under the adversarial learning idea.The goal of GANs is to estimate the potential distribution of real data samples and generate new samples from that distribution.Since their initiation, GANs have been widely studied due to their enormous prospect for applications, including image and vision computing, speech and language processing, etc. In this review paper, we summarize the state of the art of GANs and look into the future. Firstly, we survey GANs' proposal background,theoretic and implementation models, and application fields.Then, we discuss GANs' advantages and disadvantages, and their development trends. In particular, we investigate the relation between GANs and parallel intelligence,with the conclusion that GANs have a great potential in parallel systems research in terms of virtual-real interaction and integration. Clearly, GANs can provide substantial algorithmic support for parallel intelligence. | Kunfeng Wang Chao Gou Yanjie Duan Yilun Lin Xinhu Zheng Fei-Yue Wang | 2017 | IEEE/CAA Journal of Automatica Sinica2017，4，4： | 24 |
| 14 | Somatic Embryogenesis Receptor Kinases Control Root Development Mainly via Brassinosteroid-Independent Actions in Arabidopsis thaliana显示文摘Brassinosteroids(BRs),a group of plant steroidal hormones,play critical roles in many aspects of plant growth and development.Previous studies showed that BRI1-mediated BR signaling regulates cell division and differentiation during Arabidopsis root development via interplaying with auxin and other phytohormones.Arabidopsis somatic embryogenesis receptor-like kinases(SERKs),as co-receptors of BRI1,were found to play a fundamental role in an early activation step of BR signaling pathway.Here we report a novel function of SERKs in regulating Arabidopsis root development.Genetic analyses indicated that SERKs control root growth mainly via a BR-independent pathway.Although BR signaling pathway is completely disrupted in the serk1 bak1 bkk1 triple mutant,the root growth of the triple mutant is much severely damaged than the BR deficiency or signaling null mutants.More detailed analyses indicated that the triple mutant exhibited drastically reduced expression of a number of genes critical to polar auxin transport,cell cycle,endodermis development and root meristem differentiation,which were not observed in null BR biosynthesis mutant cpd and null BR signaling mutant bri1-701. | Junbo Du Hongju Yin Shasha Zhang Zhuoyun Wei Baolin Zhao Jinghua Zhang Xiaoping Gou Honghui Lin Jia Li | 2012 | Journal of Integrative Plant Biology2012，54，6： | 22 |
| 15 | Charlson comorbidity index helps predict the risk of mortality for patients with type 2 diabetic nephropathy显示文摘研究目的:探讨Charlson合并症指数(CCI)对2型糖尿病肾病死亡率的预测作用。研究方法:建立2型糖尿病肾病研究队列,根据CCI评分将患者合并症的严重程度分为三度:轻度(CCI1–2分)、中度(CCI 3–4分)、重度(CCI≥5分)。将影响死亡率的因素及按CCI分层后的组间差异指标进行Logistic回归分析及方差分析(ANOVA)。Kaplan-Meier生存曲线分析CCI指数对生存时间及死亡率的影响。重要结论:533例2型糖尿病肾病患者纳入研究。所有患者的CCI评分均大于1,44.7%(238/533)的患者死亡。患者的死亡率随CCI评分增加而增加,CCI 1–2分患者的死亡率为21.0%(50/238),CCI 3–4分患者的死亡率为56.7%(135/238),CCI≥5分患者的死亡率为22.3%(53/238)。Logistic回归分析显示CCI评分、血红蛋白和血浆白蛋白水平是患者死亡率的预测因子(P<0.05)。方差分析结果显示,与CCI评分相对较低的患者相比,CCI评分越高的患者其血红蛋白水平较低,而血清肌酐较高,死亡率也更高。Kaplan-Meier生存曲线显示CCI评分越高的患者生存时间越短。CCI评分是一种简单易行且实用的评估疾病合并症的方法,可用于预测2型糖尿病肾病患者的死亡率。关注2型糖尿病肾病患者的合并症将有利于早期、有效治疗及改善预后。 | You-qun HUANG Rong GOU Yong-shu DIAO Qing-hua YIN Wen-xing FAN Ya-ping LIANG Yi CHEN Min WU Li ZANG Ling LI Jing ZANG Lu CHENG Ping FU Fang LIU | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，1： | 23 |
| 16 | Cationic nanocarriers induce cell necrosis through impairment of Na^+/K^+-ATPase and cause subsequent inflammatory response显示文摘有积极表面费用的 Nanocarriers 以他们限制了他们的临床的应用的毒性著名。位于他们的毒性下面的机制例如煽动性的反应的正式就职,仍然保持大部分未知。在我们发现了 cationic nanocarriers 的那注射的现在的学习,包括 cationic liposomes, PEI,和 chitosan,导致了坏死的房间的快速的外观。cationic nanocarriers 导致的房间坏死依赖于他们的积极表面费用,但是不要求 RIP1 和 Mlkl。相反,细胞内部的 Na + 超载被发现伴随房间死亡。在文化媒介或有 Na +/K+-ATPase 阳离子绑定地点禁止者 ouabain 的房间的预告的处理的 Na + 的弄空,从房间坏死的保护的房间。而且,有 cationic nanocarriers 的处理在 vitro 并且在 vivo 禁止了 Na +/K+-ATPase 活动。计算模拟证明 cationic 搬运人能与 Na +/K+-ATPase 的阳离子绑定地点交往。有 ouabain 的小剂量的老鼠 pretreated 在 cationic nanocarriers 的致命的剂量的注射以后显示出改进幸存。进一步的分析建议 cationic nanocarriers 和 mitochondrial DNA 的产生的漏导致的那房间坏死能在 vivo 触发严重发炎,它被包含 TLR9 并且 MyD88 发信号的一条小径调停。一起拿,我们的结果揭示由此的新奇机制 cationic nanocarriers 通过和 mitochondrial 的随后的暴露的和 Na +/K+-ATPase, 的相互作用导致尖锐房间坏死是的联系损坏的分子的模式调停的一个关键事件煽动性的回答。我们的学习为评估 nanocarriers 的 biocompatibility 并且为药交货设计更好、更安全的有重要含意。 | Xiawei Wei Bin Shao Zhiyao He Tinghong Ye Min Luo Yaxiong Sang Xiao Liang Wei Wang Shuntao Luo Shengyong Yang Shuang Zhang Changyang Gong Maling Gou Hongxing Deng Yinglan Zhao Hanshuo Yang Senyi Deng Chengjian Zhao Li Yang Zhiyong Qian Jiong Li Xun Sun Jiahuai Han Chengyu Jiang Min Wu Zhirong Zhang | 2015 | Cell Research2015，25，2： | 21 |
| 17 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 18 | ROS activates JNK-mediated autophagy to counteract apoptosis in mouse mesenchymal stem cells in vitro显示文摘目的: 为糖尿病的可勃起的机能障碍(编辑) 的治疗的间充质的干细胞(MSC ) 的移植被移植房间的 apoptosis 妨碍。在糖尿病的编辑,有增加的氧化应力并且在阴茎海绵体,和反应的氧种类(ROS ) 不减少导致移植房间的 apoptosis。在这研究,我们检验了是否并且 autophagy 怎么涉及 MSCs.Methods 的导致 ROS 的 apoptosis : 老鼠 C3H10 MSC 与 H 2 在糖尿病的编辑房间生存能力模仿高氧化的条件的 O 2 用 MTT 被测量试金。Apoptosis 被流动 cytometry 分析。Apoptosis 相关、 autophagy 相关的蛋白质与西方的污点试金被检测。细胞内部的 autophagosome 累积用传播电子 microscopy.Results 被学习: 有 H 2 O 2(50-400 μ mol/L ) 在集中依赖者和时间依赖者礼貌禁止了房间生存能力。而且, H 2 O 2(300 μ mol/L ) 在 MSC 的导致的 apoptosis,以及激活的 autophagy。有 lysosome 禁止者 chloroquine 的预告的处理(10 μ mol/L ) 或 PI3K 禁止者 3-methyladenine (5 mmol/L ) 显著地提高了 H 2 O 2-induced 房间死亡。有 JNK 禁止者 SP600125 的预告的处理(10 μ mol/L ) 废除 H 2 LC3-II 的 O 2-induced 累积,和稀释 H 2 在 MSCs.Conclusion 的 Bcl-2 层次的 O 2-induced 减小: ROS 导致 autophagy 由 JNK 的激活在 MSC 抵抗 apoptosis。因此, autophagy 的扩大可以减少 apoptosis,延长 MSC 幸存并且改进为糖尿病的编辑的基于 MSC 的治疗学的功效。 | Guan-yu LIU Xiao-xue JIANG Xin ZHU Wei-yang HE You-lin KUANG Ke REN Yong LIN Xin GOU | 2015 | Acta Pharmacologica Sinica2015，36，12： | 20 |
| 19 | Isolation and biological analysis of tumor stem cells from pancreatic adenocarcinoma显示文摘AIM: To explore the method of isolation and biological analysis of tumor stem cells from pancreatic adenocarcinoma cell line PANC-1. METHODS: The PANC-1 cells were cultured in Dulbecco modified eagle medium F12 (1:1 volume) (DMEM-F12) supplemented with 20% fetal bovine serum (FBS). Subpopulation cells with properties of tumor stem cells were isolated from pancreatic adenocarcinoma cell line PANC-1 according to the cell surface markers CD44 and CD24 by flow cytometry. The proliferative capability of these cells in vitro were estimated by 3-[4,5-dimehyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide (MTT) method. And the tumor growth of different subpopulation cells which were injected into the hypodermisof right and left armpit of nude mice was studied,and expression of CD44 and CD24 of the CD44+CD24+ cell-formed nodules and PANC-1 cells were detected by avidin-biotin-peroxidase complex (ABC) immunohistochemical staining. RESULTS: The 5.1%-17.5% of sorted PANC-1 cells expressed the cell surface marker CD44,57.8% -70.1% expressed CD24,only 2.1%-3.5% of cells were CD44+ CD24+. Compared with CD44-CD24-cells,CD44+CD24+ cells had a lower growth rate in vitro. Implantation of 104 CD44-CD24-cells in nude mice showed no evidenttumor growth at wk 12. In contrast,large tumors were found in nude mice implanted with 103 CD44+CD24+ cells at wk 4 (2/8),a 20-fold increase in tumorigenic potential (P < 0.05 or P < 0.01). There was no obvious histological difference between the cells of the CD44+CD24+ cell-formed nodules and PANC-1 cells. CONCLUSION: CD44 and CD24 may be used as the cell surface markers for isolation of pancreatic cancer stem cells from pancreatic adenocarcinoma cell line PANC-1. Subpopulation cells CD44+CD24+ have properties of tumor stem cells. Because cancer stem cells are thought to be responsible for tumor initiation and its recurrence after an initial response to chemotherapy,it may be a very promising target for new drug development. | Peng Huang Chun-YouWang Shan-Miao Gou He-Shui Wu Tao Liu Jiang-Xin Xiong | 2008 | World Journal of Gastroenterology2008，14，24： | 19 |
| 20 | A huge oceanic-type UHP metamorphic belt in southwestern Tianshan, China: Peak metamorphic age and P-T path显示文摘Recent progress in the study of the UHP metamorphic belt in southwestern Tianshan,China,is summarized in this paper.This about 80-km-long and over 10-km-wide UHP belt has been recognized by the discovery of coesite,coesite pseudomorphs and other UHP minerals.It is the largest oceanic-type UHP metamorphic belt reported so far.It has formed due to northward subduction of the Tianshan Paleo-Ocean.U-Pb dating of metamorphic rims of zircons from a coesite-bearing garnet-phengite schist yields a peak UHP metamorphic ages of 320±3.7 Ma.Combined with ages of 233–226 Ma obtained from rims of zircons from retrograded eclogites,a long retrograde metamorphic evolution(>70 Ma)has been revealed.According to phase equilibria modeling,the P-T paths of both coesite-bearing eclogites and garnet-phengite schists are characterized by thermal relaxation,i.e.,the metamorphic temperature peak lags behind the pressure peak,indicating that the UHP rocks experienced slow and long heating and decompression during exhumation in the subduction channel.On the basis of the field observation that a small amount of eclogite lenses is wrapped in large volumes of metapelites,and the similar P-T paths of both rock types,we propose that the exhumation of the UHP eclogites from southwestern Tianshan,China,may have resulted from the exhumation of large volumes of low-density metapelites,which carried the denser eclogites to the Earth’s surface. | ZHANG LiFei DU JinXue Lü Zeng YANG Xin GOU LongLong XIA Bin CHEN ZhenYu WEI ChunJing SONG ShuGuang | 2013 | Chinese Science Bulletin2013，58，35： | 19 |
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