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| 1 | Proteolytic-antiproteolytic balance and its regulation in carcinogenesis显示文摘Cancer development is essentially a tissue remodeling process in which normal tissue is substituted with cancer tissue. A crucial role in this process is attributed toproteolytic degradation of the extracellular matrix (ECM).Degradation of ECM is initiated by proteases, secreted by different cell types, participating in tumor cell invasion and increased expression or activity of every known class of proteases (metallo-, serine-, aspartyl-, and cysteine)has been linked to malignancy and invasion of tumor cells.Proteolytic enzymes can act directly by degrading ECM or indirectly by activating other proteases, which then degrade the ECM. They act in a determined order, resulting from the order of their activation. When proteases exert their action on other proteases, the end result is a cascade leading to proteolysis. Presumable order of events in this complicated cascade is that aspartyl protease (cathepsin D) activates cysteine proteases (e.g. cathepsin B) that can activate pro-uPA. Then active uPA can convert plasminogen into plasmin. Cathepsin B as well as plasmin are capable of degrading several components of tumor stroma and may activate zymogens of matrix metalloproteinases, the main family of ECM degrading proteases. The activities of these proteases are regulated by a complex array of activators,inhibitors and cellular receptors. Tn physiological conditions the balance exists between proteases and their inhibitors.Proteolytic-antiproteolytic balance may be of major significance in the cancer development. One of the reasons for such a situation is enhanced generation of free radicals observed in many pathological states. Free radicals react with main cellular components like proteins and lipids and in this way modify proteolytic-antiproteolytic balance and enable penetration damaging cellular membrane. All these lead to enhancement of proteolysis and destruction of ECM proteins and in consequence to invasion and metastasis. | Elzbieta Skrzydlewska Mariola Sulkowska Mariusz Koda Stanislaw Sulkowski | 2005 | World Journal of Gastroenterology2005，11，9： | 14 |
| 2 | Lipid peroxidation and antioxidant status in colorectal cancer显示文摘AIM: Reactive oxygen species (ROS) can induce carcinogenesis via DNA injury. Both enzymatic and non-enzymatic parameters participate in cell protection against harmful influence of oxidative stress. The aim of the present study was to assess the levels of final lipid peroxidation products like malondialdehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) in primary colorectal cancer. Moreover, we analysed the activity of main antioxidative enzymes, superoxide dismutase (Cu, Zn-SOD),catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GSSRG-R) and the level of nonenzymatic antioxidants (glutathione, vitamins C and E).METHODS: Investigations were conducted in 81 primary colorectal cancers. As a control, the same amount of sample was collected from macroscopically unchanged colon regions of the most distant location to the cancer.Homogenisation of specimens provided 10% homogenates for our evaluations. Activity of antioxidant enzymes and level of glutathione were determined by spectrophotometry.HPLC revealed levels of vitamins C and E and served as a method to detect terminal products of lipid peroxidation in colorectal cancer.RESULTS: Our studies demonstrated a statistically significant increase in the level of lipid peroxidation products (MDA-Adc.muc.-2.65±0.48 nmol/g, Adc. G3-2.15±0.44 nmol/g, clinical Ⅳ stage 4.04±0.47 nmol/g, P<0.001 and 4-HNE-Adc.muc.-0.44±0.07 nmol/g, Adc. G3-0.44±0.10 nmol/g, clinical Ⅳstage 0.52±0.11 nmol/g, P<0.001) as well as increase of Cu,Zn-SOD (Adc.muc.-363±72 U/g, Adc. G3-318±48 U/g,clinical Ⅳ stage 421±58 U/g, P<0.001), GSH-Px (Adc.muc.-2143±623 U/g, Adc. G3-2005±591 U/g, clinical Ⅳ stage 2467±368 U/g, P<0.001) and GSSG-R (Adc. muc.-880±194 U/g,Adc. G3-795±228 U/g, dinical Ⅳ stage 951±243 U/g, P<0.001)in primary tumour comparison with normal colon (MDA1.39±0.15 nmol/g, HNE-0.29±0.03 nmol/g, Cu, Zn-SOD-117±25 U/g, GSH-Px-1723±189 U/g, GSSG-R-625±112 U/g)especially in mucinous and G3-grade adenocarcinomas as well as clinical Ⅳ stage of colorectal cancer. We also observed a decrease of CAT activity (Adc.muc. -40±14 U/g,clinical Ⅳ stage 33±18 U/g vs84±17 U/g, P<0.001) as well as a decreased level of reduced glutathione (clinical Ⅳ stage 150±48 nmol/g vs 167±15 nmol/g, P<0.05) and vitamins C and E (vit. C-clinical Ⅳ stage 325±92 nmol/g vs 513±64 nmol/g, P<0.001; vit. E-clinical Ⅳ stage 13.3±10.3nmol/g vs37.5±5.2 nmol/g).CONCLUSION: Colorectal carcinogenesis is associated with serious oxidative stress and confirms that gradual advancement of oxidative-antioxidative disorders is followed by progression of colorectal cancer. | Elzbieta Skrzydlewska Stanislaw Sulkowski Mariusz Koda Bogdan Zalewski Luiza Kanczuga-Koda Mariola Sulkowska | 2005 | World Journal of Gastroenterology2005，11，3： | 12 |
| 3 | Evaluation of serum catnepsin B and D in relation to diniGopatnological staging of colorectal cancer显示文摘AIM: Proteolytic degradation of the extracellular matrix facilitates cancer invasion and promotes metastasis. The study aims at evaluation of preoperative and postoperative serum cathepsins B and D levels in correlation with selected anatomoclinical features of colorectal cancer.METHODS: Blood samples were collected from 63colorectal cancer patients before curative operation of the tumor 10 d later. Blood that was obtained from 20healthy volunteers, served as a control. The activity of cathepsin B was measured with Bz-DL-arginine-pNA as a substrate at pH 6.0, while cathepsin D activity was determined with urea-denatured hemoglobin (pH 4.0).RESULTS: The preoperative and postoperative activities of cathepsin B were significantly (P<0.00001) lower in serum of colorectal cancer patients than in control group.However, postoperative values of this protease were significantly increased in comparison with preoperative ones (P = 0.031). Activity of cathepsin D appeared to be significantly higher in colorectal cancer sera (P<0.00001)compared with controls. No statistically significant differences between preoperative and postoperative activity of cathepsin D were noted (P = 0.09). We revealed a strong linkage of cathepsins' levels with lymph node status and pT stage of colorectal cancer.CONCLUSION: Blood serum activities of cathepsin B and D depend on the time of sampling, tumor size and lymph node involvement. Significantly, increased activity of cathepsin D could indicate a malignant condition of the large intestine. In our work, the serum postoperative decrease of cathepsin B activity appears as an obvious concomitant of local lymph node metastasis-the wellknown clinicopathological feature of poor prognosis. | Elzbieta Skrzydlewska Mariola Sulkowska Andrzej Wincewicz Mariusz Koda Stanislaw Sulkowski | 2005 | World Journal of Gastroenterology2005，11，27： | 6 |
| 4 | Regulation of Pathogen-Triggered Tryptophan Metabolism in Arabidopsis thaliana by MYB Transcription Factors and Indole Glucosinolate Conversion Products显示文摘MYB34, MYB51,和 MYB122 抄写因素作为 indolic glucosinolate (IG ) 的明确的管理者被知道有编码冗余地把色氨酸变换成 indole-3-acetaldoxime (IAOx ) 的 CYP79B2 和 CYP79B3 酶的基因的表示上的强壮的影响的生合成。这中介为 IG 生合成,和小径导致 camalexin 和吲哚羧基的酸(集成通信适配器) 代表一个分叉的点。这里,我们调查这些 MYB 怎么影响被触发病原体的 Trp 新陈代谢。我们的实验显示这三 MYB 影响不仅 IG 生产而且另外的导出 IAOx 的代谢物的组成的生合成。惊人地, PENETRATION 2 (PEN2 ) 依赖 IG 新陈代谢产品,它在 myb34/51/122 不在和 pen2 异种,为另外的导出 IAOx 的混合物的完整的调停 flg22 的正式就职是不可缺少的。然而,在病原体感染之上的集成通信适配器和 camalexin 的基因正式就职和累积没在 myb34/51/122 植物被损害,尽管有强烈减少的 IG 层次。与 cyp79B2/B3,它缺乏所有导出 IAOx 的代谢物比较,因此,我们发现 myb34/51/122 一个理想的工具对 necrotrophic 分析 IG 贡献到抵抗真菌的病原体 Plectosphaerella cucumerina。myb34/51/122 的危险性类似于 pen2 的,但是很比 cyp79B2/B3 的危险性降低,显示 MYB34/51/122 贡献向 P 的抵抗。专门通过 IG 生合成的 cucumerina,和那 PEN2 是响应微生物引起的病原体激活 IG 的主要的叶 myrosinase。 | Henning Frerigmann Mariola Pislewska-Bednarek Andrea Sanchez-Vallet Antonio Molina Erich Glawischnig Tamara Gigolashvili Pawel Bednarek | 2016 | Molecular Plant2016，9，5： | 5 |
| 5 | Connexin 26 correlates with Bcl-xL and Bax proteins expression in colorectal cancer显示文摘AIM: To evaluate of Cx26 in correlation with Bcl-xL and Bax proteins in colorectal cancer.METHODS: Immunohistochemical staining using specific antibodies was performed to evaluate the protein expression of Cx26, Bax and Bcl-xL in 152 colorectal cancer samples and the correlations among studied proteins as well as the relationships between the expression of Cx26,Bax, Bcl-xL and clinicopathological features were analyzed.RESULTS: Both normal epithelial cells and carcinoma cells expressed Cx26, Bax and Bcl-xL, but Cx26 in cancer cells showed aberrant, mainly cytoplasmic staining. Expression of Cx26, Bax and Bcl-xL was observed in 55.9%, 55.5%and 72.4% of evaluated colorectal cancers respectively.We found the positive correlation between Cx26 and Bax expression (r = 0.561, P<0.0001), Cx26 and Bcl-xL (r = 0.409, P<0.0001) as well as between Bax and Bcl-xL (r = 0.486, P<0.0001). Association of Cx26, Bax and Bcl-xL expression with histological G2 grade of tumors was noted (P<0.005, P<0.001 and P<0.002 respectively).CONCLUSION: Cytoplasmic presence of Cx26 and its association with apoptotic markers could indicate a distinct role from physiological functions of Cx26 in cancer cells and it could suggest that connexins might be a target point for modulations of apoptosis with therapeutic implications. | Luiza Kanczuga-Koda Stanislaw Sulkowski Mariusz Koda Elzbieta Skrzydlewska Mariola Sulkowska | 2005 | World Journal of Gastroenterology2005，11，10： | 5 |
| 6 | Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion显示文摘Development of a novel in vivo lung perfusion(IVLP)procedure allows localized delivery of high-dose doxorubicin(DOX)for targeting residual micrometastatic disease in the lungs.However,DOX delivery via IVLP requires careful monitoring of drug level to ensure tissue concentrations of this agent remain in the therapeutic window.A small dimension nitinol wire coated with a sorbent of biocompatible morphology(Bio-SPME)has been clinically evaluated for in vivo lung tissue extraction and determination of DOX and its key metabolites.The in vivo Bio-SPME-IVLP experiments were performed on pig model over various(150 and 225 mg/m^(2))drug doses,and during human clinical trial.Two patients with metastatic osteosarcoma were treated with a single 5 and 7 μg/mL(respectively)dose of DOX during a 3-h IVLP.In both pig and human cases,DOX tissue levels presented similar trends during IVLP.Human lung tissue concentrations of drug ranged between 15 and 293 μg/g over the course of the IVLP procedure.In addition to DOX levels,Bio-SPME followed by liquid chromatography-mass spectrometry analysis generated 64 metabolic features during endogenous metabolite screening,providing information about lung status during drug administration.Real-time monitoring of DOX levels in the lungs can be performed effectively throughout the IVLP procedure by in vivo Bio-SPME chemical biopsy approach.Bio-SPME also extracted various endogenous molecules,thus providing a real-time snapshot of the physiology of the cells,which might assist in the tailoring of personalized treatment strategy. | Barbara Bojko Nikita Looby Mariola Olkowicz Anna Roszkowska Bogumiła Kupcewicz Pedro Reck dos Santos Khaled Ramadan Shaf Keshavjee Thomas K.Waddell German Goomez-Rios Marcos Tascon Krzysztof Gorynski Marcelo Cypel Janusz Pawliszyn | 2021 | Journal of Pharmaceutical Analysis2021，11，1： | 3 |
| 7 | Vapour Treatment Method Against Other Pyro-and Hydrometallurgical Processes Applied to Recover Platinum From Used Auto Catalytic Converters显示文摘Today more and more cars are produced every year. All of them have to be equipped with catalytic converters, the main role of which is to obtain substances harmless to the environment instead of exhausted gases. Catalytic converters contain platinum group metals (PGM) especially platinum, palladium and rhodium. The price of these metals and their increasing demand are the reasons why today it is necessary to recycle used auto catalytic converters. There are many available methods of recovering PGM metals from them, especially platinum. These methods used mainly hydrometallurgical processes; however pyrometallurgical ones become more and more popular. The article presents results of the research mainly concerning pyrometallurgical processes. Two groups of research were carried out. In the first one different metals such as lead, magnesium and copper were used as a metal collector. During the tests, platinum went to those metals forming an alloy. In other research metal vapours were blown through catalytic converter carrier (grinded or whole). In the tests metals such as calcium, magnesium, cadmium and zinc were applied. As a result white or grey powder (metal plus platinum) was obtained. The tables present results of the research. Processing parameters and conclusions are also shown. To compare efficiency of pyrometallurgical and hydrometallurgical methods catalytic converter carrier and samples of copper with platinum obtained from pyrometallurgical method were solved in aqua regia, mixture of aqua regia and fluoric acid. | Agnieszka FORNALCZYK Mariola SATERNUS | 2013 | Acta Metallurgica Sinica(English Letters)2013，26，3： | 2 |
| 8 | Taste and mouth-feel properties of different types of tannin-like polyphenolic compounds and anthocyanins in wine显示文摘 | Stéphane Vidal Leigh Francis Ann Noble Mariola Kwiatkowski Véronique Cheynier Elizabeth Waters | 2003 | Analytica Chimica Acta2003，，1： | 2 |
| 9 | Application of mathematical models to the calculation of ultrafihration flux in water treatment 显示文摘 | Mariola Rajca Michal Bodzek Krystyna Konieczny | 2009 | Desalination2009，239，123： | 1 |
| 10 | Thermally rearranged(TR)Poly(ether-benzoxazole)mMembranes for gas sep-aration显示文摘 | Mariola Calle Young Moo Lee | 2011 | Macromolecules2011，44，： | 1 |
| 11 | Activity and effective connectivity of parietal and occipital cortical regions during haptic shape perception 显示文摘 | Pehier S Stilla R Mariola E | 2007 | Neuropsychologia2007，45，3： | 1 |
| 12 | HealthAgents: distributed multi-agent brain tumor diagnosis and?prognosis显示文摘 | Horacio González-Vélez Mariola Mier Margarida Julià-Sapé Theodoros N. Arvanitis Juan M. García-Gómez Montserrat Robles Paul H. Lewis Srinandan Dasmahapatra David Dupplaw Andrew Peet Carles Arús Bernardo Celda Sabine Huffel Magí Lluch-Ariet | 2009 | Applied Intelligence2009，，3： | 1 |
| 13 | Increased Lipid Peroxidation and Ascorbic Acid Utilization in Testis and Epididymis of Rats Chronically Exposed to Lead显示文摘 | Mariola Marchlewicz Barbara Wiszniewska Boles?aw Gonet Irena Baranowska-Bosiacka Krzysztof Safranow Agnieszka Kolasa Wojciech G??bowski Rafa? Kurzawa Katarzyna Jakubowska Monika E. Ra? | 2007 | BioMetals2007，，1： | 1 |
| 14 | Prenatal diagnosis of vasa previa显示文摘 | Grzegorz H. Br&eogon borowicz Wies&lstrok aw Markwitz Agata Szpera-Go&zacute dziewicz Anna Dera-Szymanowska Mariola Ropacka-Lesiak Piotr Szyma&nacute ski Beata Kubiaczyk-Paluch | 2014 | The Journal of Maternal-Fetal & Neonatal Medicine2014，，： | 1 |
| 15 | Kinetics of fulvic and humic acids photodegradation in water solutions 显示文摘 | Mariola R Micha B | 2013 | Sepa- ration and Purification Technology2013，120，： | 1 |
| 16 | Losing ground:farmland preservation,economic utilitarianism,and the erosion of the agrarian ideal显示文摘 | Mariola M J | | 0，，02： | 1 |
| 17 | Tactile Discrimination of Grating Orientation : fMRI Activation Patterns 显示文摘 | ZHANG M MARIOLA E STILLA R | 2005 | Human Brain Mapping2005，25，： | 1 |
| 18 | Brand Communities on the Internet: A Case Study of Coca-Cola's Spanish Virtual Community显示文摘 | Maria Sicilia Mariola Palazo'n | 2008 | Corporate Communications: An International Journal2008，13，3： | 1 |
| 19 | Immunohistochemical localization of metallothionein and p53 protein in pancreatic serous cystadenomas显示文摘 | Mariola ?liwińska-Mossoń MD Ph.D. Halina Milnerowicz Jerzy Rabczyński Stanis?aw Milnerowicz | 2009 | Archivum Immunologiae et Therapiae Experimentalis2009，，4： | 1 |
| 20 | Soluble Nogo-A, an inhibitor of axonal regeneration, as a biomarker for multiple sclerosis显示文摘 | Anna Jurewicz Mariola Matysiak Cedric S. Raine Krzysztof Selmaj | 2007 | Neurology2007，，： | 1 |
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