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| 1 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 2 | Autoantibody to MOG suggests two distinct clinical subtypes of NMOSD显示文摘We characterized a unique group of patients with neuromyelitis optica spectrum disorder(NMOSD) who carried autoantibodies of aquaporin-4(AQP4) and myelin-oligodendrocyte glycoprotein(MOG). Among the 125 NMOSD patients, 10(8.0%) were AQP4- and MOG-ab double positive, and 14(11.2%) were MOG-ab single positive. The double-positive patients had a multiphase disease course with a high annual relapse rate(P=0.0431), and severe residual disability(P<0.0001). Of the double-positive patients, 70% had MS-like brain lesions, more severe edematous, multifocal regions on spinal magnetic resonance imaging(MRI), pronounced decreases of retinal nerve fiber layer thickness and atrophy of optic nerves. In contrast, patients with only MOG-ab had a higher ratio of monophasic disease course and mild residual disability. Spinal cord MRI illustrated multifocal cord lesions with mild edema, and brain MRIs showed more lesions around lateral ventricles. NMOSD patients carrying both autoantibodies to AQP4 and MOG existed and exhibited combined features of prototypic NMO and relapsing-remitting form of MS, whereas NMOSD with antibodies to MOG only exhibited an 'intermediate' phenotype between NMOSD and MS. Our study suggests that antibodies against MOG might be pathogenic in NMOSD patients and that determination of anti-MOG antibodies maybe instructive for management of NMOSD patients. | Yaping Yan Yujing Li Ying Fu Li Yang Lei Su Kaibin Shi Minshu Li Qiang Liu Aimee Borazanci Yaou Liu Yong He Jeffrey L Bennett Timothy L Vollmer Fu-Dong Shi | 2016 | Science China(Life Sciences)2016，59，12： | 20 |
| 3 | Economic Dispatch of Power Systems with Virtual Power Plant Based Interval Optimization Method显示文摘Load prediction and power prediction uncertainties are inevitable aspects of a virtual power plant(VPP).In power system economic dispatch(ED)modeling,the interval is used to describe prediction uncertainties.An ED model with interval uncertainty is established in this paper.The probability degree definition is adopted to convert the interval-based economic dispatch model into a deterministic model for the purposes of solving the modeling problem.Simulation tests are performed on a 10-machine system using professional optimization software(LINGO).The simulation results verify the validity of the proposed interval-based scheme for the economic dispatch of a power system with VPP. | Chongxin Huang Dong Yue Jun Xie Yaping Li Ke Wang | 2016 | CSEE Journal of Power and Energy Systems2016，2，1： | 17 |
| 4 | L-3-n-butylphthalide protects against vascular dementia via activation of the Akt kinase pathway显示文摘As a neuroprotective drug for the treatment of ischemic stroke, 3-n-butylphthalide, a celery seed extract, has been approved by the State Food and Drug Administration of China as a clinical therapeutic drug for ischemic stroke patients. L-3-n-butylphthalide possesses significant efficacy in the treatment of acute ischemic stroke. The activated Akt kinase pathway can prevent the death of nerve cells and exhibit neuroprotective effects in the brain after stroke. This study provides the hypothesis that l-3-n-butylphthalide has a certain therapeutic effect on vascular dementia, and its mechanism depends on the activation of the Akt kinase pathway. A vascular dementia mouse model was established by cerebral repetitive ischemia/reperfusion, and intragastrically administered l-3-n-butylphthalide daily for 28 consecutive days after ischemia/reperfusion, or 7 consecutive days before ischemia/reperfusion. The Morris water maze test showed significant impairment of spatial learning and memory at 4 weeks after operation, but intragastric administration of l-3-n-butylphthalide, especially pretreatment with l-3-n-butylphthalide, significantly reversed these changes. Thionine staining and western blot analylsis showed that preventive and therapeutic application of l-3-n-butylphthalide can reduce loss of pyramidal neurons in the hippocampal CA1 region and alleviate nerve damage in mice with vascular demen-tia. In addition, phosphorylated Akt expression in hippocampal tissue increased significantly after l-3-n-butylphthalide treatment. Experimental findings demonstrate that l-3-n-butylphthalide has preventive and therapeutic effects on vascular dementia, and its mechanism may be mediated by upregulation of phosphorylated Akt in the hippocampus. | Yaping Huai Yanhong Dong Jing Xu Nan Meng Chunfeng Song Wenbin Li Peiyuan Lv | 2013 | Neural Regeneration Research2013，8，19： | 17 |
| 5 | Overexpression of SGR Results in Oxidative Stress and Lesion-mimic Cell Death in Rice Seedlings显示文摘It is thought that the Stay Green Rice(SGR)gene is involved in the disaggregation of the light harvesting complex and in the subsequent breakdown of chlorophyll and apo-protein during senescence.In this study,we found that overexpression of SGR(Ov-SGR)resulted in the generation of singlet oxygen and other reactive oxygen species and produced a chlorophyll-dependent regional cell death phenotype on leaves of rice seedlings.Transcriptome analyses using Affymetrix Rice GeneChips revealed that Ov-SGR rice seedlings exhibited a number of signs of singlet oxygen response.The genes and their associated biochemical pathways identified provide an insight into how rice plants respond to singlet oxygen at the molecular and physiologic level. | Huawu Jiang Yaping Chen Meiru Li Xingian Xu Guojiang Wu | 2011 | Journal of Integrative Plant Biology2011，53，5： | 16 |
| 6 | Rapid growth cost in “all-fish” growth hormone gene transgenic carp:Reduced critical swimming speed显示文摘Evidence has accumulated that there is a trade-off between benefits and costs associated with rapid growth. A trade-off between growth rates and critical swimming speed (Ucrit) had been also reported to be common in teleost fish. We hypothesize that growth acceleration in the F3 generation of 'all-fish' growth hormone gene (GH) transgenic common carp (Cyprinus carpio L.) would reduce the swimming abilities. Growth and swimming performance between transgenic fish and non-transgenic controls were compared. The results showed that transgenic fish had a mean body weight 1.4―1.9-fold heavier,and a mean specific growth rate (SGR) value 6%―10% higher than the controls. Transgenic fish,however,had a mean absolute Ucrit (cm/s) value 22% or mean relative Ucrit (BL/s) value 24% lower than the controls. It suggested that fast-growing 'all-fish' GH-transgenic carp were inferior swimmers. It is also supported that there was a trade-off between growth rates and swimming performance,i.e. faster-growing individuals had lower critical swimming speed. | LI DeLiang FU CuiZhang HU Wei ZHONG Shan WANG YaPing ZHU ZuoYan | 2007 | Chinese Science Bulletin2007，52，11： | 14 |
| 7 | The application of geostatistics in grain size trend analysis: A case study of eastern Beibu Gulf显示文摘有 71 件表面沉积样品,从东方 Beibu 海湾收集。片刻参数(即吝啬的尺寸,排序并且偏斜度) 在使用谷物尺寸分析以后被获得。geostatistical 分析然后被使用为这些参数学习空间自相关;当时范围,在 semivariogram 的一个参数米空间自相关的规模,被估计。结果显示为排序系数的范围身体上是有意义的。从谷物尺寸趋势分析模型计算的趋势向量根据以前的研究与年度海洋发行量模式和沉积运输率一致。因此,从吝啬的尺寸的 semivariogram 导出的范围能在谷物尺寸趋势分析被用作典型距离,它可以移开基于经验或严峻的方法得到典型距离的传统的方法引起的偏爱。因此从分析能也在未来为沉积采样密度的决心为有用信息提供的 geostatistical 的结果回答工作。 | MA Fei WANG Yaping LI Yan YE Changjiang XU Zhiwei ZHANG Fan | 2010 | Journal of Geographical Sciences2010，20，1： | 14 |
| 8 | Aquaporin-dependent excessive intrauterine fluid accumulation is a major contributor in hyper-estrogen induced aberrant embryo implantation显示文摘 | Ying Zhang Qi Chen He Zhang Qiang Wang Rong Li Yaping Jin Haibin Wang Tonghui Ma Jie Qiao Enkui Duan | 2015 | Cell Research2015，25，1： | 14 |
| 9 | Fingolimod alters inflammatory mediators and vascular permeability in intracerebral hemorrhage显示文摘Intracerebral hemorrhage(ICH) leads to high rates of death and disability. The pronounced infl ammatory reactions that rapidly follow ICH contribute to disease progression. Our recent clinical trial demonstrated that oral administration of an immune modulator fingolimod restrained secondary injury derived from initial hematoma,but the mechanisms remain unknown. In this study,we aim to investigate the effects of fingolimod on inflammatory mediators and vascular permeability in the clinical trial of oral fingolimod for intracerebral hemorrhage(ICH). The results showed that fingolimod decreased the numbers of circulating CD4^+ T,CD8^+ T,CD(19)^+B,NK,and NKT cells and they recovered quickly after the drug was stopped. The plasma ICAM level was decreased and IL-10 was increased by fingolimod. Interestingly,fingolimod protected vascular permeability as indicated by a decreased plasma level of MMP9 and the reduced r T1%. In conclusion,modulation of systemic inflammation by fingolimod demonstrates that it is an effective therapeutic agent for ICH. Fingolimod may prevent perihematomal edema enlargement by protecting vascular permeability. | Yu-Jing Li Guo-Qiang Chang Yuanchu Liu Ye Gong Chunsheng Yang Kristofer Wood Fu-Dong Shi Ying Fu Yaping Yan | 2015 | Neuroscience Bulletin2015，31，6： | 12 |
| 10 | Phosphorus oxoanion-intercalated layered double hydroxides for high-performance oxygen evolution显示文摘合理设计和为氧进化反应(OER ) 的有效、划算的电极的控制制造为探讨崭新的精力危机是批评的。有特定的夹层阴离子的镍铁分层的双氢氧化物(NiFe-LDHs )( 即磷酸盐,亚磷酸盐,和次磷酸盐) 被一个一起沉淀方法制作并且作为氧进化 electrocatalysts 调查了。磷 oxoanion 的置闰在一个碱的答案提高了 OER 活动;最佳的表演(即, 215 mV, 37.7 mV/dec 的一个小 Tafel 斜坡,和稳定的电气化学的行为的一个低发作潜力) 与设置次磷酸盐的 NiFe-LDH 催化剂被完成,在传统的设置碳酸盐的 NiFe-LDH 上的示威戏剧的改进以活动和耐久性。这提高的表演被归因于在设置的磷的 oxoanions 和分享边的瞬间 6 之间的相互作用(M = Ni, Fe ) 层,它修改表面 Ni 地点的电子结构。这个概念应该为更有效的基于 LDH 的氧进化 electrocatalysts 的设计是鼓舞人心的。 | Ma Luo Zhao Cai Cheng Wang Yongmin Bi Li Qian Yongchao Hao Li Li Yun Kuang Yaping Li Xiaodong Lei Ziyang HUO Wen Liu Hailiang Wang Xiaoming Sun Xue Duan | 2017 | Nano Research2017，10，5： | 12 |
| 11 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 12 | Effects of Saikosaponin-D on syndecan-2,matrix metalloproteinases and tissue inhibitor of metalloproteinases-2 in rats with hepatocellular carcinoma显示文摘OBJECTIVE:To investigate effects of Saikosaponin D(SSd) on syndecan-2,matrix metalloproteinases(MMPs) and tissue inhibitor of metalloproteinases-2(TIMP-2) in livers of rat with hepatocellular carcinoma(HCC).METHODS:Male SD rats were divided into control(n=10),model(n=20) and SSd(n=20) groups,and model and SSd groups given intragastric 0.2%(w/v) N-diethylnitrosamine to induce HCC.SSd group received 0.03%(w/v) SSd in saline.Liver samples were analysed immunohistochemically for syndecan-2,MMP-2,MMP-13 and TIMP-2 at 16 weeks.RESULTS:The model group had more malignant nodules than the SSd group;all model-group HCC cells were grade III;SSd-group HCC cells were grades I-II.Controls showed normal hepatic cell phenotypes and no syndecan-2 + staining.Syndecan-2 + staining was greater in the model group(35.2%,P≤0.001) than in controls or the SSd group(16.5%,P ≤ 0.001).The model group had more intense MMP-2 + staining than controls(0.37 vs 0.27,P≤0.01) or the SSd group(0.31 vs 0.37,P≤0.05);and higher MMP-13 + staining(72.55%) than in controls(12.55%,P≤0.001) and SSd group(20.18%,P≤0.01).The model group also had more TIMP-2 + staining(57.2%) than controls(20.9%,P≤0.001) and SSd group(22.7%,P≤0.001).Controls and SSd group showed no difference in TIMP-2 + rates.CONCLUSION:SSd inhibited HCC development,and downregulated expression of syndecan-2,MMP-2,MMP-13 and TIMP-2 in rat HCC liver tissue. | Xiaoli Jia Shuangsuo Dang Yanan Cheng Xin Zhang Mei Li Yaping Li Siyuan Li | 2012 | Journal of Traditional Chinese Medicine2012，32，3： | 12 |
| 13 | Characteristics of strike-slip inversion structures of the Karatau fault and their petroleum geological significances in the South Turgay Basin,Kazakhstan显示文摘The Karatau fault is one of the important strike-slip faults in central Asia,and the South Turgay Basin is located towards its northern end.Detailed seismic interpretation indicated that the strikeslip tectonism of the Karatau fault weakened gradually from west to east in the South Turgay Basin.Typical flower structures developed on the section,and strike-slip faults showed an echelon pattern on planar view.The Karatau strike-slip fault affected the South Turgay Basin in two periods:(1) The South Turgay strike-slip pull-apart rift basin formed as a result of regional extensive stress in the Early-Middle Jurassic,characterized by the juxtaposition of horsts and grabens.The formation of horsts provided favorable reservoir spaces for later hydrocarbon accumulation,and different filling stages of grabens controlled different reservoir-forming factors in grabens.(2) Two stages of tectonic inversion occurred in the Late Jurassic and Late Cretaceous and played a crucial role in the final shape of the structure in the South Turgay Basin.The oil and gas migrated to form reservoirs and mainly concentrated in the horsts,graben slopes and in both sides of the strike-slip fault zone.In the case of the degree of accumulation of petroleum,the factor explaining why horsts are better than grabens is the strike-slip pull-apart of the South Turgay Basin,and the structure inversion of the South Turgay Basin explains why the west graben is better than the east one.Overall,the Karatau strike-slip fault played a very important role in the formation of the South Turgay Basin and its hydrocarbon accumulations. | Yin Wei Fan Zifei Zheng Junzhang Yin Jiquan Zhang Mingjun Sheng Xiaofeng Guo Jianjun Li Qiyan Lin yaping | 2012 | Petroleum Science2012，9，4： | 11 |
| 14 | Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing显示文摘The regulation of stomatal lineage cell development has been extensively investigated.However,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been reported.In this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis seedlings.We identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker genes.Comparative analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother cells.Examination of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these genes.Collectively,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development. | Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing Sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo Jean-David Rochaix Yuchen Miao Xuwu Sun | 2020 | Molecular Plant2020，13，8： | 11 |
| 15 | Sedimentation and morphological changes at Yuantuojiao Point, estuary of the North Branch, Changjiang River显示文摘The North Branch, separated by the Chongming Island, was once the main channel in the estuary of the Changjiang River. Reclamation and a decrease in runoff to the North Branch had led to the narrowing and shallowing of the channel. The Yuantuojiao Point is located at the intersecting point connecting the North Branch of the Changjiang River and the Jiangsu coastline. Erosion cliffs are developed between the typical silty-muddy tidal flat and the salt marsh occupied by Spartina alterniflorea, and this has changed rapidly over the past few years. The sediment grain size analysis results of the surficial and two core samples indicate that the Yuantuojiao Point tidal flat experienced continuous accretional processes. Based upon 137 Cs analysis results of the YT and YY Cores sampled from the tidal flat at the Yuantuojiao Point, the average sedimentation rate of the YT Core was 2.30 cm/a from 1963 to 2007, and 2.38 cm/a from 1954 to 2007 for the YY Core. The sedimentation rates of both core locations have declined since the 1960s corresponding to the seaward reclamation at the Yuantuojiao Point. The average sedimentation rates at the Yuantuojiao Point were similar to that of the silty-muddy tidal flat at the northern Jiangsu coast, but lower than that of the south of the Changjiang River Estuary. According to field morphological investigations from 2006 to 2008 on the salt marsh at the Yuantuojiao Point, cliffs retreated markedly by storm surges and disappeared gradually because of the rapid sedimentation on the silty-muddy tidal flat. The maximum annual retreat reached 10 m. The recent sedimentation and morphological changes of the Yuantuojiao Point tidal flat not only displayed the retreat of the salt marsh and the disappearance of cliffs, but also was accompanied by rapid sedimentation of the silty-muddy tidal flat and the salt marsh, indicating the responses to the tidal currents, storm surges, Spartina alterniflorea trapping sediments and large-scale reclamation. The sediment grain size and their trends, southward coastal flow, and sandspits of the longshore bars suggest that the main sediment source at the Yuantuoijao Point, estuary of the North Branch was possibly from the Changjiang River before 1958, since then, it has been from the south of the submarine radial sand ridges of the southern Huanghai Sea (Yellow Sea). | XIE Li ZHANG Zhenke ZHANG Yunfeng WANG Yaping HUANG Xianjin | 2013 | Acta Oceanologica Sinica2013，32，2： | 11 |
| 16 | Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)-associated endonuclease 9(CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T_0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T_0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T_0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding. | Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie Wang Xingchun Wang Changjie Yan Kejian Wang | 2017 | Science China(Life Sciences)2017，60，5： | 11 |
| 17 | The dimension and morphology of alveolar bone at maxillaryanterior teeth in periodontitis: a retrospective analysis usingCBCT显示文摘The morphology of the alveolar bone at the maxillary anterior teeth in periodontitis patients was evaluated by cone-beam computed tomography(CBCT) to investigate the distribution of alveolar defects and provide guidance for clinical practice. Ninety periodontitis patients and 30 periodontally healthy individuals were selected to determine the morphology of the alveolar bone at the maxillary anterior teeth according to the degree of bone loss, tooth type, sex and age. The differences in the dimensions between periodontitis patients and healthy individuals were compared, and the distribution of alveolar bone defects was analyzed.A classification system was established regarding the sagittal positions and angulations of the teeth. The buccal residual bone was thicker and the lingual bone was thinner in the periodontitis patients than in the periodontally healthy individuals, and there were differences between the different tooth types, sexes and age subgroups. The buccal undercut was close to the alveolar ridge, while fenestration was reduced and the apical bone height was higher in periodontitis patients than in periodontally healthy individuals.The apical bone height increased with the aggravation of bone loss and age. The proportions of different sagittal positions changed with the aggravation of bone loss. Moreover, the teeth moved more buccally regarding the positions of the maxillary anterior teeth.The morphology of the alveolar bone at the maxillary anterior teeth differed between periodontitis patients and healthy individuals,and the differences were related to the degree of bone loss, tooth type, sex and age. | Xue Zhang Yuchao Li Ziming Ge Haijiao Zhao Lei Miao Yaping Pan | 2020 | International Journal of Oral Science2020，12，1： | 10 |
| 18 | High efficient gene targeting in rice field eel Monopterus albus by transcription activator-like effector nucleases显示文摘Genome editing plays an important role in the research of gene functions,gene correction and cell replacement therapy.Traditionally,genome editing is mainly dependent on the technology of homologous recombination(HR)and embryonic stem(ES)cell lines in mammals[1].However,genome editing is severely limited by the low efficiency of HR and lack of ES cell lines in most organisms.Recently,the development of zinc finger nucleases(ZFNs), | Ke Feng Hongrui Luo Yongming Li Ji Chen Yaping Wang Yonghua Sun Zuoyan Zhu Wei Hu | 2017 | Science Bulletin2017，62，3： | 9 |
| 19 | Thermodynamic modeling and phase diagram prediction of salt lake brine systems.Ⅰ. Aqueous Mg^2+–Ca^2+–Cl^- binary and ternary systems显示文摘Salt lake brine is a complex salt-water system under natural environment.Although many models can express the thermodynamic properties and phase equilibrium of electrolyte aqueous solution,the multi-temperature characteristics and predictability are still the goals of model development.In this study,a comprehensive thermodynamic model system is re-established based on the eNRTL model and some improvements:(1) new expression of long-range electrostatic term with symmetrical reference state is proposed to handle the electrolyte solution covering entire concentration range;(2) the temperature dependence of the binary interaction parameters is formulated with a Gibbs Helmholtz expression containing three temperature coefficients,the liquid parameters,which associated with Gibbs energy,enthalpy,and heat capacity contribution;and(3) liquid parameters and solid species data are regressed from properties and solubility data at full temperature range.Together the activity coefficient model,property models and parameters of liquid and solid offer a comprehensive thermodynamic model system for the typical bittern of MgCl2-CaCl2-H2 O binary and ternary systems,and it shows excellent agreement with the literature data for the ternary and binary systems.The successful prediction of complete phase diagram of ternary system shows that the model has the ability to deal with high concentration and high non-idealitv system,and the ability to extrapolate the temperature. | Huan Zhou Xiaolong Gu Yaping Dai Jingjing Tang Jian Guo Guangbi Li Xiaoqin Bai | 2020 | Chinese Journal of Chemical Engineering2020，28，9： | 9 |
| 20 | Genetic diversity of Tricholoma matsutake in Yunnan Province显示文摘To investigate the genetic diversity of Tricholoma matsutake,we studied ITS and IGS1 sequences and PCR polymorphism of a retrotransposon in 56 fruit bodies collected from 13 counties of 9 regions in Yunnan Province. We found one and three haplotypes based on ITS and IGS1 sequences,respectively. Moreover,there was no significant difference in PCR polymorphism of the retrotransposon among different populations. Compared with Jilin Province(China) and Japanese populations,although Yun-nan was highly homogenous to Japanese populations,low genetic diversity of T. matsutake in Yunnan did not support the view that this species originated from Yunnan. | SHA Tao ZHANG HanBo DING HuaSun LI ZongJu CHENG LiZhong ZHAO ZhiWei ZHANG YaPing | 2007 | Chinese Science Bulletin2007，52，9： | 8 |
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