|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Tauroursodeoxycholic acid(TUDCA) inhibits influenza A viral infection by disrupting viral proton channel M2显示文摘Influenza is a persistent threat to human health and there is a continuing requirement for updating antiinfluenza strategies. Initiated by observations of different endoplasmic reticulum(ER) responses of host to seasonal H1N1 and highly pathogenic avian influenza(HPAI) A H5N1 infections, we identified an alternative antiviral role of tauroursodeoxycholic acid(TUDCA), a clinically available ER stress inhibitor, both in vitro and in vivo. Rather than modulating ER stress in host cells, TUDCA abolished the proton conductivity of viral M2 by disrupting its oligomeric states, which induces inefficient viral infection. We also showed that M2 penetrated cells, whose intracellular uptake depended on its proton channel activity,an effect observed in both TUDCA and M2 inhibitor amantadine. The identification and application of TUDCA as an inhibitor of M2 proton channel will expand our understanding of IAV biology and complement current anti-IAV arsenals. | Ning Li Yanxu Zhang Shuangxiu Wu Ruodan Xu Zhiqing Li Jindong Zhu Hongliang Wang Xiao Li Mingyao Tian Huijun Lu Ningyi Jin Chengyu Jiang | 2019 | Science Bulletin2019，64，3： | 6 |
| 2 | Molecular and serological surveillance of Getah virus in the Xinjiang Uygur Autonomous Region,China,2017–2020显示文摘The Getah virus(GETV),a mosquito-borne RNA virus,is widely distributed in Oceania and Asia.GETV is not the only pathogenic to horses,pigs,cattle,foxes and boars,but it can also cause fever in humans.Since its first reported case in Chinese mainland in 2017,the number of GETV-affected provinces has increased to seventeen till now.Therefore,we performed an epidemiologic investigation of GETV in the Xinjiang region,located in northwestern China,during the period of 2017-2020.ELISA was used to analyze 3299 serum samples collected from thoroughbred horse,local horse,sheep,goat,cattle,and pigs,with thoroughbred horse(74.8%),local horse(67.3%),goat(11.7%),sheep(10.0%),cattle(25.1%)and pigs(51.1%)being positive for anti-GETV antibodies.Interestingly,the neutralizing antibody titer in horses was much higher than in other species.Four samples from horses and pigs were positive for GETV according to RT-PCR.Furthermore,from the serum of a local horse,we isolated GETV which was designated as strain XJ-2019-07,and determined its complete genome sequence.From the phylogenetic relationships,it belongs to the Group III lineage.This is the first evidence of GETV associated to domestic animals in Xinjiang.Overall,GETV is prevalent in Xinjiang and probably has been for several years.Since no vaccine against GETV is available in China,detection and monitoring strategies should be improved in horses and pigs,especially imported and farmed,in order to prevent economic losses. | Ning Shi Xiangshu Qiu Xinyu Cao Zhanhai Mai Xiangyu Zhu Nan Li He Zhang Jinyong Zhang Zhuoxin Li Nuerlan Shaya Huijun Lu Ningyi Jin | 2022 | Virologica Sinica2022，37，2： | 5 |
| 3 | Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18显示文摘 | Guoshun Shen Ningyi Jin Mingxiao Ma Kuoshi Jin Min Zheng Tianzhong Zhuang Huijun Lu Guangze Zhu Hongtao Jin Minglan Jin Xiaowei Huo Xiaoguang Qin Ronglan Yin Chang Li Hongwen Li Yang Li Zhenzhen Han Yifeng Chen Miaomiao Jin | 2007 | Vaccine2007，，21： | 2 |
| 4 | Efficacy of seasonal pandemic influenza hemagglutinin DNA vaccines delivered by electroporation against aseasonal H1N1 virus challenge in mice显示文摘Prophylactic DNA vaccines against the influenza virus are promising alternatives to conventional vaccines.In this study,we generated two candidate gene-based influenza vaccines encoding either the seasonal or pandemic hemagglutinin antigen(HA) from the strains A/New Caledonia/20/99(H1N1)(pV1A5) and A/California/04/2009(H1N1)(pVEH1) ,respectively.After verifying antigen expression,the immunogenicity of the vaccines delivered intramuscularly with electroporation was tested in a mouse model.Sera of immunized animals were tested in hemagglutination inhibition assays and by ELISA for the presence of HA-specific antibodies.HA-specific T-cells were also measured in IFN-γELISpot assays.The protective efficacy of the candidate influenza vaccines was evaluated by measuring mortality rates and body weight after a challenge with 100 LD50 of mouse-adapted A/New Caledonia/20/99(H1N1) .Mice immunized with either one of the two vaccines showed significantly higher T cell and humoral immune responses(P<0.05) than the pVAX1 control group.Additionally,the pV1A5 vaccine effectively protected the mice against a lethal homologous mouse-adapted virus challenge with a survival rate of 100%compared with a 40%survival rate in the pVEH1 vaccinated group(P<0.05) .Our study indicates that the seasonal influenza DNA vaccine completely protects against the homologous A/New Caledonia/20/99 virus(H1N1) ,while the pandemic influenza DNA vaccine only partially protects against this virus. | TAN Lei LU HuiJun ZHANG Dan WANG KaiYan TIAN MingYao LIU CunXia LIU YanYu HU Bo JIN NingYi | 2011 | Science China(Life Sciences)2011，54，4： | 2 |
| 5 | Failure characteristics of Ag-SnO_2 contact material in AC level显示文摘The failure characteristics of the contact material Ag-SnO2 were investigated by X-ray diffraction(XRD),scanning electron microscopy(SEM),and X-ray energy dispersive spectroscopy(EDS).Phase analysis showed that there were Ag,SnO2,and Sn on the contact surfaces at the time of failure under the load condition of 30 A and 220 V(AC),which means that SnO2 decomposed in service.When the contacts were tested under airproof ambient condition,half-spherical bulges came into being on the contact surfaces,which resulted in early failure and dispersive characteristics.But half-spherical bulges were not found when the contacts were tested under open ambient condition.The temperature change of the contacts during the life testing under airproof ambient condition was different from that under open ambient condition. | MENG Fanbin LU Jianguo ZHU Yongqing LU Ningyi CHEN Zhichao HUANG Qiutao | 2008 | Rare Metals2008，27，1： | 2 |
| 6 | Orally administered BZL-s RNA-20 oligonucleotide targeting TLR4 effectively ameliorates acute lung injury in mice显示文摘The global COVID-19 pandemic emerged at the end of December 2019.Acute respiratory distress syndrome(ARDS)and acute lung injury(ALI)are common lethal outcomes of bacterial lipopolysaccharide(LPS),avian influenza virus,and SARS-Co V-2.Toll-like receptor 4(TLR4)is a key target in the pathological pathway of ARDS and ALI.Previous studies have reported that herbal small RNAs(s RNAs)are a functional medical component.BZL-s RNA-20(Accession number:B59471456;Family ID:F2201.Q001979.B11)is a potent inhibitor of Toll-like receptor 4(TLR4)and pro-inflammatory cytokines.Furthermore,BZLs RNA-20 reduces intracellular levels of cytokines induced by lipoteichoic acid(LTA)and polyinosinic-polycytidylic acid(poly(I:C)).We found that BZL-s RNA-20 rescued the viability of cells infected with avian influenza H5N1,SARS-Co V-2,and several of its variants of concern(VOCs).Acute lung injury induced by LPS and SARS-Co V-2 in mice was significantly ameliorated by the oral medical decoctosome mimic(bencaosome;sphinganine(d22:0)+BZL-s RNA-20).Our findings suggest that BZLs RNA-20 could be a pan-anti-ARDS/ALI drug. | Dandan Zhao Yuhao Qin Jiaqi Liu Kegong Tang Shuaiyao Lu Zirui Liu Yexuan Lin Cong Zhang Fengming Huang Jiahui Chang Chang Li Mingyao Tian Yiming Ma Xiaoyun Li Congzhao Zhou Xiao Li Xiaozhong Peng Ningyi Jin Chengyu Jiang | 2023 | Science China(Life Sciences)2023，66，7： | 1 |
| 7 | Immune responses of swine inoculated with a recombinant fowlpox virus co-expressing P12A and 3C of FMDV and swine IL-18显示文摘 | Mingxiao Ma Ningyi Jin Guoshun Shen Guangze Zhu Hui Juan Liu Min Zheng Huijun Lu Xiaowei Huo Minglan Jin Gefen Yin Haili Ma Xu Li Yue Ji Kuoshi Jin | 2007 | Veterinary Immunology and Immunopathology2007，，1： | 1 |
| 8 | Construction and immunogenicity of a recombinant fowlpox virus containing the capsid and 3C protease coding regions of foot-and-mouth disease virus显示文摘 | Min Zheng Ningyi Jin Hongyong Zhang Minglan Jin Huijun Lu Minxiao Ma Chang Li Gefen Yin Ruilin Wang Qi Liu | 2006 | Journal of Virological Methods2006，，1： | 1 |
| 9 | Avian influenza viruses suppress innate immunity by inducingtrans-transcriptional readthrough via SSU72显示文摘Innate immunity plays critical antiviral roles. The highly virulent avian influenza viruses (AIVs) H5N1, H7N9, and H5N6 can betterescape host innate immune responses than the less virulent seasonal H1N1 virus. Here, we report a mechanism by whichtranscriptional readthrough (TRT)-mediated suppression of innate immunity occurs post AIV infection. By using cell lines, mouselungs, and patient PBMCs, we showed that genes on the complementary strand (“trans” genes) influenced by TRT were involved inthe disruption of host antiviral responses during AIV infection. The trans-TRT enhanced viral lethality, and TRT abolishmentincreased cell viability and STAT1/2 expression. The viral NS1 protein directly bound to SSU72, and degradation of SSU72 inducedTRT. SSU72 overexpression reduced TRT and alleviated mouse lung injury. Our results suggest that AIVs infection induce TRT byreducing SSU72 expression, thereby impairing host immune responses, a molecular mechanism acting through the NS1-SSU72-trans-TRT-STAT1/2 axis. Thus, restoration of SSU72 expression might be a potential strategy for preventing AIV pandemics. | Yan Zhao Fengming Huang Zhen Zou Yuhai Bi Yang Yang Cong Zhang Qiang Liu Daozhen Shang Yiwu Yan Xiangwu Ju Song Mei Peng Xie Xiao Li Mingyao Tian Shuguang Tan Huijun Lu Zongsheng Han Kangtai Liu Yuqing Zhang Junbo Liang Zhu Liang Qingchao Zhang Jiahui Chang William JLiu Cong Feng Tanshi Li Michael Q.Zhang Xiaoyue Wang George FGao Yingxia Liu Ningyi Jin Chengyu Jiang | 2022 | Cellular & Molecular Immunology2022，19，6： | 0 |
| 10 | Newcastle disease virus suppresses antigen presentation via inhibiting IL-12 expression in dendritic cells显示文摘As a potential vectored vaccine,Newcastle disease virus(NDV)has been subject to various studies for vaccine development,while relatively little research has outlined the immunomodulatory effect of the virus in antigen presentation.To elucidate the key inhibitory factor in regulating the interaction of infected dendritic cells(DCs)and T cells,DCs were pretreated with the NDV vaccine strain LaSota as an inhibitor and stimulated with lipopolysaccharide(LPS)for further detection by enzyme-linked immunosorbent assay(ELISA),flow cytometry,immunoblotting,and quantitative real-time polymerase chain reaction(qRT-PCR).The results revealed that NDV infection resulted in the inhibition of interleukin(IL)-12p40 in DCs through a p38 mitogen-activated protein kinase(MAPK)-dependent manner,thus inhibiting the synthesis of IL-12p70,leading to the reduction in T cell proliferation and the secretion of interferon-(IFN-),tumor necrosis factor-α(TNF-α),and IL-6 induced by DCs.Consequently,downregulated cytokines accelerated the infection and viral transmission from DCs to T cells.Furthermore,several other strains of NDV also exhibited inhibitory activity.The current study reveals that NDV can modulate the intensity of the innate-adaptive immune cell crosstalk critically toward viral invasion improvement,highlighting a novel mechanism of virus-induced immunosuppression and providing new perspectives on the improvement of NDV-vectored vaccine. | Fulong NAN Wenlong NAN Xin YAN Hui WANG Shasha JIANG Shuyun ZHANG Zhongjie YU Xianjuan ZHANG Fengjun LIU Jun LI Xiaoqiong ZHOU Delei NIU Yiquan LI Wei WANG Ning SHI Ningyi JIN Changzhan XIE Xiaoni CUI He ZHANG Bin WANG Huijun LU | 2024 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2024，25，3： | 0 |
| 11 | Corrigendum to'Tauroursodeoxycholic acid(TUDCA)inhibits influenza A viral infection by disrupting viral proton channel M2'[Sci.Bull.,64(3)(2019)180-188]显示文摘Following the published article[1].the authors noticed an error duplication of image of DAPI in Fig.2c'AF'and'No pretreated and without TUDCA'.The correct DAPI image was in the merged image of'No pretreated and without TUDCA'of the published article.Therefore,the corrected Fig.2c should be as follows:The online version of the original artice can be found at http://gffzzd3cc09b8251d45dfskonpu5wbuoqf6bvb.ffgz.tsg.suse.edu.cn/10.101/j.scib.2018.08.0131. | Ning Li Yanxu Zhang Shuangxiu Wu Ruodan Xu Zhiqing Li Jindong Zhu Hongliang Wang Xiao Li Mingyao Tian Huijun Lu Ningyi Jin Chengyu Jiang | 2021 | Science Bulletin2021，66，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员