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您的检索式：作者名="Xiaoni CUI"
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| 1 | Establishment of organoid models based on a nested array chip for fast and reproducible drug testing in colorectal cancer therapy显示文摘The conventional microwell-based platform for construction of organoid models exhibits limitations in precision oncology applications because of low-speed growth and high variability. Here, we established organoid models on a nested array chip for fast and reproducible drug testing using 50% matrigel. First, we constructed mouse small intestinal and colonic organoid models. Compared with the conventional microwell-based platform, the mouse organoids on the chip showed accelerated growth and improved reproducibility due to the nested design of the chip. The design of the chip provides miniaturized and uniform shaping of the matrigel that allows the organoid to grow in a concentrated and controlled manner. Next, a patient-derived organoid(PDO) model from colorectal cancer tissues was successfully generated and characterized on the chip. Finally, the PDO models on the chip, from three patients, were implemented for high-throughput drug screening using nine treatment regimens. The drug sensitivity testing on the PDO models showed good quality control with a coefficient of variation under 10% and a Z’ factor of more than 0.7. More importantly, the drug responses on the chip recapitulate the heterogeneous response of individual patients, as well as showing a potential correlation with clinical outcomes. Therefore,the organoid model coupled with the nested array chip platform provides a fast and reproducible means for predicting drug responses to accelerate precise oncology. | Yancheng Cui Rongrong Xiao Yushi Zhou Jianchuang Liu Yi Wang Xiaodong Yang Zhanlong Shen Bin Liang Kai Shen Yi Li Geng Xiong Yingjiang Ye Xiaoni Ai | 2022 | Bio-Design and Manufacturing2022，5，4： | 0 |
| 2 | Newcastle disease virus suppresses antigen presentation via inhibiting IL-12 expression in dendritic cells显示文摘As a potential vectored vaccine,Newcastle disease virus(NDV)has been subject to various studies for vaccine development,while relatively little research has outlined the immunomodulatory effect of the virus in antigen presentation.To elucidate the key inhibitory factor in regulating the interaction of infected dendritic cells(DCs)and T cells,DCs were pretreated with the NDV vaccine strain LaSota as an inhibitor and stimulated with lipopolysaccharide(LPS)for further detection by enzyme-linked immunosorbent assay(ELISA),flow cytometry,immunoblotting,and quantitative real-time polymerase chain reaction(qRT-PCR).The results revealed that NDV infection resulted in the inhibition of interleukin(IL)-12p40 in DCs through a p38 mitogen-activated protein kinase(MAPK)-dependent manner,thus inhibiting the synthesis of IL-12p70,leading to the reduction in T cell proliferation and the secretion of interferon-(IFN-),tumor necrosis factor-α(TNF-α),and IL-6 induced by DCs.Consequently,downregulated cytokines accelerated the infection and viral transmission from DCs to T cells.Furthermore,several other strains of NDV also exhibited inhibitory activity.The current study reveals that NDV can modulate the intensity of the innate-adaptive immune cell crosstalk critically toward viral invasion improvement,highlighting a novel mechanism of virus-induced immunosuppression and providing new perspectives on the improvement of NDV-vectored vaccine. | Fulong NAN Wenlong NAN Xin YAN Hui WANG Shasha JIANG Shuyun ZHANG Zhongjie YU Xianjuan ZHANG Fengjun LIU Jun LI Xiaoqiong ZHOU Delei NIU Yiquan LI Wei WANG Ning SHI Ningyi JIN Changzhan XIE Xiaoni CUI He ZHANG Bin WANG Huijun LU | 2024 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2024，25，3： | 0 |
| 3 | Polygalacin D inhibits the growth of hepatocellular carcinoma cells through BNIP3L-mediated mitophagy and endogenous apoptosis pathways显示文摘Platycodon grandiflorum(Jacq.)A.DC.is a famous medicinal plant commonly used in East Asia.Triterpene saponins isolated from P.grandiflorum are the main biologically active compounds,among which polygalacin D(PGD)has been reported to be an anti-tumor agent.However,its anti-tumor mechanism against hepatocellular carcinoma is unknown.This study aimed to explore the inhibitory effect of PGD in hepatocellular carcinoma cells and related mechanisms of action.We found that PGD exerted significant inhibitory effect on hepatocellular carcinoma cells through apoptosis and autophagy.Analysis of the expression of apoptosis-related proteins and autophagy-related proteins revealed that this phenomenon was attributed to the mitochondrial apoptosis and mitophagy pathways.Subsequently,using specific inhibitors,we found that apoptosis and autophagy had mutually reinforcing effects.In addition,further analysis of autophagy showed that PGD induced mitophagy by increasing BCL2 interacting protein 3 like(BNIP3L)levels.In vivo experiments demonstrated that PGD significantly inhibited tumor growth and increased the levels of apoptosis and autophagy in tumors.Overall,our findings showed that PGD induced cell death of hepatocellular carcinoma cells primarily through mitochondrial apoptosis and mitophagy pathways.Therefore,PGD can be used as an apoptosis and autophagy agonist in the research and development of antitumor agents. | NAN Fulong NAN Wenlong YU Zhongjie WANG Hui CUI Xiaoni JIANG Shasha ZHANG Xianjuan LI Jun WANG Zhifei ZHANG Shuyun WANG Bin LI Yiquan | 2023 | Chinese Journal of Natural Medicines2023，21，5： | 0 |
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