|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Herbal decoctosome is a novel form of medicine显示文摘Traditionally, herbal medicine is consumed by drinking decoctions produced by boiling herbs with water. The functional components of the decoction are heat stable. Small RNAs(sRNAs) were reported as a new class of functional components in decoctions. However, the mechanisms by which sRNAs survive heat treatment of the decoction and enter cells are unclear.Previous studies showed that plant-derived exosome-like nanoparticles(ELNs), which we call botanosomes, could deliver therapeutic reagents in vivo. Here, we report that heat-stable decoctosomes(ELNs) from decoctions have more therapeutic effects than the decoctions in vitro and demonstrate therapeutic efficacy in vivo. Furthermore, sRNAs, such as HJT-sRNA-m7 and PGY-sRNA-6, in the decoctosome exhibit potent anti-fibrosis and anti-inflammatory effects, respectively. Decoctosome is comprised of lipids, chemical compounds, proteins, and s RNAs. A medical decoctosome mimic is called bencaosome. A single lipid sphinganine(d22:0) identified in the decoctosome was mixed and heated with the synthesized sRNAs to form the simplest bencaosome. This simple bencaosome structure was identified by critical micelle concentration(cmc) assay that sRNAs coassembled with sphinganine(d22:0) to form the lipid layers of vesicles. The heating process facilitates co-assembly of sRNAs and sphinganine(d22:0) until a steady state is reached. The artificially produced sphinganine-HJT-sRNA-m7 and sphinganinePGY-sRNA-6 bencaosomes could ameliorate bleomycin-induced lung fibrosis and poly(I:C)-induced lung inflammation, respectively, following oral administration in mice. Our study not only demonstrates that the herbal decoctosome may represent a combinatory remedy in precision medicine but also provides an effective oral delivery route for nucleic acid therapy. | Xiaoyun Li Zhu Liang Jianchao Du Zhiqing Wang Song Mei Zhiqing Li Yan Zhao DANDan Zhao Yiming Ma Jun Ye Jiantao Xu Yu Zhao Jiahui Chang Yuhao Qin Lanlan Yu Chenxuan Wang Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 26 |
| 2 | Plant-derived phosphocholine facilitates cellular uptake of anti-pulmonary fibrotic HJT-sRNA-m7显示文摘Pulmonary fibrosis, a progressive chronic disease with a high mortality rate, has limited treatment options. Currently, lung transplantation remains the only effective treatment. Here we report that a small RNA, HJT-sRNA-m7, from a Chinese herbal medicine Hong Jing Tian(HJT, RHODIOHAE CRENULATAE RADIX ET RHIZOMA, Rhodiola crenulata) can effectively reduce the expressions of fibrotic hallmark genes and proteins both in alveolar in vitro and in mouse lung tissues in vivo. We also discovered over one hundred oil-soluble chemicals from HJT decoctions, most of which are found in lipid extracts from other Chinese herbals decoctions, including Pu Gong Ying(PGY, TARAXACI HERBA, Taraxacum mongolicum), Chuan Xin Lian(CXL, changed to 'ANDROGRAPHIS HERBA, Andrographis paniculata'), and Jin Yin Hua(JYH, lonicera japonica or Honeysuckle). We identified the active component in these decoctions as two forms of phosphocholines, PC(18:0/18:2) and PC(16:0/18:2). These PCs potentially could form liposomes with small RNAs to enter human alveolar and gastric cells. Our experimental results suggest an unprecendent lipid complex route through which botanic small RNA can enter human bodies.Our results provide an innovative treatment strategy for oral delivery of siRNAs as therapeutic medication. | Jianchao Du Zhu Liang Jiantao Xu Yan Zhao Xiaoyun Li Yanli Zhang DANDan Zhao Ruxuan Chen Yang Liu Trupti Joshi Jiahui Chang Zhiqing Wang Yanxu Zhang Jindong Zhu Qiang Liu Dong Xu Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 21 |
| 3 | Development of ensemble learning models to evaluate the strength of coal-grout materials显示文摘In the loose and fractured coal seam with particularly low uniaxial compressive strength(UCS),driving a roadway is extremely difficult as roof falling and wall spalling occur frequently.To address this issue,the jet grouting(JG)technique(high-pressure grout mixed with coal particles)was first introduced in this study to improve the self-supporting ability of coal mass.To evaluate the strength of the jet-grouted coal-grout composite(JG composite),the UCS evolution patterns were analyzed by preparing 405 specimens combining the influential variables of grout types,curing time,and coal to grout(C/G)ratio.Furthermore,the relationships between UCS and these influencing variables were modeled using ensemble learning methods i.e.gradient boosted regression tree(GBRT)and random forest(RF)with their hyperparameters tuned by the particle swarm optimization(PSO).The results showed that the chemical grout composite has higher short-term strength,while the cement grout composite can achieve more stable strength in the long term.The PSO-GBRT and PSO-RF models can both achieve high prediction accuracy.Also,the variable importance analysis demonstrated that the grout type and curing time should be considered carefully.This study provides a robust intelligent model for predicting UCS of JG composites,which boosts JG design in the field. | Yuantian Sun Guichen Li Nong Zhang Qingliang Chang Jiahui Xu Junfei Zhang | 2021 | International Journal of Mining Science and Technology2021，31，2： | 6 |
| 4 | A Survey of Bitmap Index Compression Algorithms for Big Data显示文摘With the growing popularity of Internet applications and the widespread use of mobile Internet, Internet traffic has maintained rapid growth over the past two decades. Internet Traffic Archival Systems(ITAS) for packets or flow records have become more and more widely used in network monitoring, network troubleshooting, and user behavior and experience analysis. Among the three key technologies in ITAS, we focus on bitmap index compression algorithm and give a detailed survey in this paper. The current state-of-the-art bitmap index encoding schemes include: BBC, WAH, PLWAH, EWAH, PWAH, CONCISE, COMPAX, VLC, DF-WAH, and VAL-WAH. Based on differences in segmentation, chunking, merge compress, and Near Identical(NI) features, we provide a thorough categorization of the state-of-the-art bitmap index compression algorithms. We also propose some new bitmap index encoding algorithms, such as SECOMPAX, ICX, MASC, and PLWAH+, and present the state diagrams for their encoding algorithms. We then evaluate their CPU and GPU implementations with a real Internet trace from CAIDA. Finally, we summarize and discuss the future direction of bitmap index compression algorithms. Beyond the application in network security and network forensic, bitmap index compression with faster bitwise-logical operations and reduced search space is widely used in analysis in genome data, geographical information system, graph databases, image retrieval, Internet of things, etc. It is expected that bitmap index compression will thrive and be prosperous again in Big Data era since 1980s. | Zhen Chen Yuhao Wen Junwei Cao Wenxun Zheng Jiahui Chang Yinjun Wu Ge Ma Mourad Hakmaoui Guodong Peng | 2015 | Tsinghua Science and Technology2015，20，1： | 5 |
| 5 | Erratum to:Herbal decoctosome is a novel form of medicine显示文摘Following the published article,we noticed an error duplication in Figure 5G“control”and“PGY-6”that was introduced during the revised process,with an attempt to replace it with higher-resolution images.Here we provide the original data in the first submitted manuscript(Figure 5G). | Xiaoyun Li Zhu Liang Jianchao Du Zhiqing Wang Song Mei Zhiqing Li Yan Zhao Dandan Zhao Yiming Ma Jun Ye Jiantao Xu Yu Zhao Jiahui Chang Yuhao Qin Lanlan Yu Chenxuan Wang Chengyu Jiang | 2020 | Science China(Life Sciences)2020，63，9： | 2 |
| 6 | PMN-MDSCs modulated by CCL20 from cancer cells promoted breast cancer cell stemness through CXCL2-CXCR2 pathway显示文摘Our previous studies have showed that C-C motif chemokine ligand 20(CCL20)advanced tumor progression and enhanced the chemoresistance of cancer cells by positively regulating breast cancer stem cell(BCSC)self-renewal.However,it is unclear whether CCL20 affects breast cancer progression by remodeling the tumor microenvironment(TME).Here,we observed that polymorphonuclear myeloid-derived suppressor cells(PMN-MDSCs)were remarkably enriched in TME of CCL20-overexpressing cancer cell orthotopic allograft tumors.Mechanistically,CCL20 activated the differentiation of granulocyte-monocyte progenitors(GMPs)via its receptor C-C motif chemokine receptor 6(CCR6)leading to the PMN-MDSC expansion.PMN-MDSCs from CCL20-overexpressing cell orthotopic allograft tumors(CCL20-modulated PMN-MDSCs)secreted amounts of C-X-C motif chemokine ligand 2(CXCL2)and increased ALDH+BCSCs via activating CXCR2/NOTCH1/HEY1 signaling pathway.Furthermore,C-X-C motif chemokine receptor 2(CXCR2)antagonist SB225002 enhanced the docetaxel(DTX)effects on tumor growth by decreasing BCSCs in CCL20high-expressing tumors.These findings elucidated how CCL20 modulated the TME to promote cancer development,indicating a new therapeutic strategy by interfering with the interaction between PMN-MDSCs and BCSCs in breast cancer,especially in CCL20high-expressing breast cancer. | Rui Zhang Mengxue Dong Juchuanli Tu Fengkai Li Qiaodan Deng Jiahui Xu Xueyan He Jiajun Ding Jie Xia Dandan Sheng Zhaoxia Chang Wei Ma Haonan Dong Yi Zhang Lixing Zhang Lu Zhang Suling Liu | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 2 |
| 7 | Poly (butylene succinate)-based biocomposites filled with polysac- charide nanocrystals: Structure and properties 显示文摘 | Lin Ning Yu Jiahui Chang Peter R Li Junli Huang Jin | 2011 | Polymer Composites2011，32，3： | 1 |
| 8 | Orally administered BZL-s RNA-20 oligonucleotide targeting TLR4 effectively ameliorates acute lung injury in mice显示文摘The global COVID-19 pandemic emerged at the end of December 2019.Acute respiratory distress syndrome(ARDS)and acute lung injury(ALI)are common lethal outcomes of bacterial lipopolysaccharide(LPS),avian influenza virus,and SARS-Co V-2.Toll-like receptor 4(TLR4)is a key target in the pathological pathway of ARDS and ALI.Previous studies have reported that herbal small RNAs(s RNAs)are a functional medical component.BZL-s RNA-20(Accession number:B59471456;Family ID:F2201.Q001979.B11)is a potent inhibitor of Toll-like receptor 4(TLR4)and pro-inflammatory cytokines.Furthermore,BZLs RNA-20 reduces intracellular levels of cytokines induced by lipoteichoic acid(LTA)and polyinosinic-polycytidylic acid(poly(I:C)).We found that BZL-s RNA-20 rescued the viability of cells infected with avian influenza H5N1,SARS-Co V-2,and several of its variants of concern(VOCs).Acute lung injury induced by LPS and SARS-Co V-2 in mice was significantly ameliorated by the oral medical decoctosome mimic(bencaosome;sphinganine(d22:0)+BZL-s RNA-20).Our findings suggest that BZLs RNA-20 could be a pan-anti-ARDS/ALI drug. | Dandan Zhao Yuhao Qin Jiaqi Liu Kegong Tang Shuaiyao Lu Zirui Liu Yexuan Lin Cong Zhang Fengming Huang Jiahui Chang Chang Li Mingyao Tian Yiming Ma Xiaoyun Li Congzhao Zhou Xiao Li Xiaozhong Peng Ningyi Jin Chengyu Jiang | 2023 | Science China(Life Sciences)2023，66，7： | 1 |
| 9 | General seismic wave and phase detection software driven by deep learning显示文摘We developed an automatic seismic wave and phase detection software based on PhaseNet,an efficient and highly generalized deep learning neural network for P-and S-wave phase picking.The software organically combines multiple modules including application terminal interface,docker container,data visualization,SSH protocol data transmission and other auxiliary modules.Characterized by a series of technologically powerful functions,the software is highly convenient for all users.To obtain the P-and S-wave picks,one only needs to prepare threecomponent seismic data as input and customize some parameters in the interface.In particular,the software can automatically identify complex waveforms(i.e.continuous or truncated waves)and support multiple types of input data such as SAC,MSEED,NumPy array,etc.A test on the dataset of the Wenchuan aftershocks shows the generalization ability and detection accuracy of the software.The software is expected to increase the efficiency and subjectivity in the manual processing of large amounts of seismic data,thereby providing convenience to regional network monitoring staffs and researchers in the study of Earth's interior. | Ming Zhao Jiahui Ma Hao Chang Shi Chen | 2021 | Earthquake Research Advances2021，1，3： | 1 |
| 10 | Stretchable polymer composites with ultrahigh piezoelectric performance显示文摘Flexible piezoelectric materials capable of withstanding large deformation play key roles in flexible electronics.Ferroelectric ceramics with a high piezoelectric coefficient are inherently brittle,whereas polar polymers exhibit a low piezoelectric coefficient.Here we report a highly stretchable/compressible piezoelectric composite composed of ferroelectric ceramic skeleton,elastomer matrix and relaxor ferroelectric-based hybrid at the ceramic/matrix interface as dielectric transition layers,exhibiting a giant piezoelectric coefficient of 250 picometers per volt,high electromechanical coupling factor keff of 65%,ultralow acoustic impedance of 3MRyl and high cyclic stability under 50%compression strain.The superior flexibility and piezoelectric properties are attributed to the electric polarization and mechanical load transfer paths formed by the ceramic skeleton,and dielectric mismatch mitigation between ceramic fillers and elastomer matrix by the dielectric transition layer.The synergistic fusion of ultrahigh piezoelectric properties and superior flexibility in these polymer composites is expected to drive emerging applications in flexible smart electronics. | Tongxiang Tang Zhonghui Shen Jian Wang Shiqi Xu Jiaxi Jiang Jiahui Chang Mengfan Guo Youjun Fan Yao Xiao Zhihao Dong Houbing Huang Xiaoyan Li Yihui Zhang Danyang Wang Long-Qing Chen Ke Wang Shujun Zhang Ce-Wen Nan Yang Shen | 2023 | National Science Review2023，10，8： | 1 |
| 11 | HOXC4 up-regulates NF-κB signaling and promotes the cell proliferation to drive development of human hematopoiesis, especially CD43+ cells显示文摘The hematopoietic function of HOXC4 has not been extensively investigated.Our research indicated that induction of HOXC4 in co-culture system from D10 significantly promoted productions of most hematopoietic progenitor cells.CD34−CD43+cells could be clearly classified into CD34−CD43^(low) and CD34−CD43^(high) sub-populations at D14.The former cells had greater myelogenic potential,and their production was not significantly influenced by induction of HOXC4.By contrast,the latter cells had greater potential to differentiate into megakaryocytes and erythroid cells,and thus had properties of erythroid–megakaryocyte common progenitors,which abundance was increased by∼2-fold when HOXC4 was induced from D10.For CD34−CD43^(low),CD34+CD43+,and CD34−CD43^(high) sub-populations,CD43 level served as a natural index for the tendency to undergo hematopoiesis.Induction of HOXC4 from D10 caused more CD43+cells sustain in S-phase with up-regulation of NF-κB signaling,which could be counteracted by inhibition of NF-κB signaling.These observations suggested that promotion of hematopoiesis by HOXC4 is closely related to NF-κB signaling and a change in cell-cycle status,which containing potential of clinical applications. | Jiahui Zeng Wencui Sun Jing Chang Danying Yia Lijiao Zhu Yonggang Zhang Xu Pan Ya Zhou Mowen Lai Guohui Bian Qiongxiu Zhou Jiaxin Liu Bo Chen FengMa | 2020 | Blood Science2020，2，4： | 1 |
| 12 | A Kaposi’s sarcoma-associated herpes virus-encoded microRNA contributes to dilated cardiomyopathy显示文摘Dilated cardiomyopathy(DCM)is the leading cause of heart transplantation.By microRNA(miRNA)array,a Kaposi’s sarcoma-associated herpes virus(KSHV)-encoded miRNA,kshv-miR-K12-1-5p,was detected in patients with DCM.The KSHV DNA load and kshv-miR-K12-1-5p level in plasma from 696 patients with DCM were measured and these patients were followed-up. | Yanru Zhao Huaping Li Hengzhi Du Zhongwei Yin Mengying He Jiahui Fan Xiang Nie Yang Sun Huiying Hou Beibei Dai Xudong Zhang Yuanyuan Cai Kunying Jin Nan Ding Zheng Wen Jiang Chang Chen Chen Dao Wen Wang | 2023 | Signal Transduction and Targeted Therapy2023，8，7： | 1 |
| 13 | Multi-scale Analysis of the Relationships between Solar Activity, CO_(2) and Global Surface Temperature显示文摘To reveal whether the dynamics of solar activity precede those of global temperature, especially in terms of global warming, the relationship between total solar irradiance(TSI), which is treated as a proxy of solar activity, and global surface temperature(GST) is investigated in the frequency domain using wavelet coherence. The results suggest that the effect of TSI on GST is mainly reflected on the characteristic scale around 22 yr, and variations in TSI lead to changes in GST with some delay effect as shown by the phase difference arrows. However, this implicated relationship has been perturbed by excessive CO_(2) emissions since 1960. Through the combination of co-integration analysis and wavelet coherence, the hidden relationship between TSI and GST has been uncovered without the CO_(2) effect and the results further indicate that TSI has a positive effect on GST at the characteristic scale around 22 yr with a 3 yr lead. | Zhen Li Lijun Chang Jiahui Lou Yi Shen Haoming Yan | 2022 | Research in Astronomy and Astrophysics2022，22，9： | 0 |
| 14 | Spatiotemporal characteristics of seed rain and soil seed bank of artificial Caragana korshinskii Kom. forest in the Tengger Desert, China显示文摘Vegetation restoration and reconstruction are effective approaches to desertification control and achieving social and economic sustainability in desert areas.However,the self-succession ability of native plants during the later periods of vegetation restoration remains unclear.Therefore,this study was conducted to bridge the knowledge gap by investigating the regeneration dynamics of artificial forest under natural conditions.The information of seed rain and soil seed bank was collected and quantified from an artificial Caragana korshinskii Kom.forest in the Tengger Desert,China.The germination tests were conducted in a laboratory setting.The analysis of species quantity and diversity in seed rain and soil seed bank was conducted to assess the impact of different durations of sand fixation(60,40,and 20 a)on the progress of vegetation restoration and ecological conditions in artificial C.korshinskii forest.The results showed that the top three dominant plant species in seed rain were Echinops gmelinii Turcz.,Eragrostis minor Host.,and Agropyron mongolicum Keng.,and the top three dominant plant species in soil seed bank were E.minor,Chloris virgata Sw.,and E.gmelinii.As restoration period increased,the density of seed rain and soil seed bank increased first and then decreased.While for species richness,as restoration period increased,it gradually increased in seed rain but decreased in soil seed bank.There was a positive correlation between seed rain density and soil seed bank density among all the three restoration periods.The species similarity between seed rain or soil seed bank and aboveground vegetation decreased with the extension of restoration period.The shape of the seeds,specifically those with external appendages such as spines and crown hair,clearly had an effect on their dispersal,then resulting in lower seed density in soil seed bank.In addition,precipitation was a crucial factor in promoting rapid germination,also resulting in lower seed density in soil seed bank.Our findings provide valuable insights for guiding future interventions during the later periods of artificial C.korshinskii forest,such as sowing and restoration efforts using unmanned aerial vehicles. | SHEN Jianxiang WANG Xin WANG Lei WANG Jiahui QU Wenjie ZHANG Xue CHANG Xuanxuan YANG Xinguo CHEN Lin QIN Weichun ZHANG Bo NIU Jinshuai | 2024 | Journal of Arid Land2024，16，4： | 0 |
| 15 | Comparative Study on the Policy of Multiplicity Issues in Clinical Trials at Home and Abroad显示文摘Objective To study the content of China’s guiding principles on multiplicity issues in clinical trials,and to provide reference for the revision of China’s relevant guiding principles.Methods Based on ICH E9,the similarities and differences of the guiding principles of US Food and Drug Administration(FDA),European Medicines Agency(EMA),and National Medical Products Administration(NMPA)on the multiplicity issues in clinical trials were compared one by one.Results and Conclusion In general,NMPA guidelines are based on ICH E9,but in detail,the guidelines of FDA and EMA focus differently on the multiplicity issues.Therefore,NMPA guidelines need to be detailed and comprehensive.NMPA guidelines can be refined by referring to foreign guidelines to improve the practical guiding significance for clinical research and promote the level of domestic clinical trials in line with international standards. | Gong Jingran Chang Yingnan Qiao Jiahui Chen Yuwen | 2024 | Asian Journal of Social Pharmacy2024，19，1： | 0 |
| 16 | Overexpression of HOXA9 upregulates NF-κB signaling to promote human hematopoiesis and alter the hematopoietic differentiation potentials显示文摘Background: The HOX genes are master regulators of embryogenesis that are also involved inhematopoiesis. HOXA9 belongs to a cluster of HOX genes that play extensively studied roles inhematopoiesis and leukemogenesis.Methods: We established HOXA9-inducible human embryonic stem cells (HOXA9/hESCs) with normalpluripotency and potential for hematopoiesis, which could be used to analyze gene function with highaccuracy. HOXA9/hESCs co-cultured with aorta–gonad–mesonephros-derived stromal cells (AGM-S3) wereinduced to overexpress HOXA9 with doxycycline (DOX) at various times after hematopoiesis started andthen subjected to flow cytometry.Results: Induction of HOXA9 from Day 4 (D4) or later notably promoted hematopoiesis and also increasedthe production of CD34+ cells and derived populations. The potential for myelogenesis was significantlyelevated while the potential for erythrogenesis was significantly reduced. At D14, a significant promotion ofS phase was observed in green fluorescent protein positive (GFP+) cells overexpressing HOXA9. NF-κBsignaling was also up-regulated at D14 following induction of HOXA9 on D4. All of these effects could becounteracted by addition of an NF-κB inhibitor or siRNA against NFKB1 along with DOX.Conclusions: Overexpression of HOXA9 starting at D4 or later during hematopoiesis significantly promotedhematopoiesis and the production of myeloid progenitors while reduced the production of erythroidprogenitors, indicating that HOXA9 plays a key role in hematopoiesis and differentiation of hematopoieticlineages. | Jiahui Zeng Danying Yi Wencui Sun Yuanlin Liu Jing Chang Lijiao Zhu Yonggang Zhang Xu Pan Yong Dong Ya Zhou Mowen Lai Guohui Bian Qiongxiu Zhou Jiaxin Liu Bo Chen Feng Ma | 2021 | Cell Regeneration2021，10，1： | 0 |
| 17 | A wake-up call: signaling in regulating ornamental geophytes dormancy显示文摘Ornamental geophytes are a group of important flowers worldwide.As perennial plants,geophytes go through several rounds of life cycle under seasonal climates.The dormant trait of underground modified organs in geophytes is critical for the process of storage,planting as well as breeding.Although the dormant physiology in geophytes is complex and largely unknown,several advancements have been achieved in this field.Here,we review the knowledge on the role of environmental factors,endogenous hormones,carbohydrates,and epigenetics in the regulation of geophytes dormancy release(GDR).We also discuss dormancy release(DR)methods and their roles in geophytes,including small molecular chemicals and wounding treatments. | Yajie Zhao Chang Liu Juanjuan Sui Jiahui Liang Jintao Ge Jingru Li Wenqiang Pan Mingfang Yi Yunpeng Du Jian Wu | 2022 | Ornamental Plant Research2022，2，1： | 0 |
| 18 | Avian influenza viruses suppress innate immunity by inducingtrans-transcriptional readthrough via SSU72显示文摘Innate immunity plays critical antiviral roles. The highly virulent avian influenza viruses (AIVs) H5N1, H7N9, and H5N6 can betterescape host innate immune responses than the less virulent seasonal H1N1 virus. Here, we report a mechanism by whichtranscriptional readthrough (TRT)-mediated suppression of innate immunity occurs post AIV infection. By using cell lines, mouselungs, and patient PBMCs, we showed that genes on the complementary strand (“trans” genes) influenced by TRT were involved inthe disruption of host antiviral responses during AIV infection. The trans-TRT enhanced viral lethality, and TRT abolishmentincreased cell viability and STAT1/2 expression. The viral NS1 protein directly bound to SSU72, and degradation of SSU72 inducedTRT. SSU72 overexpression reduced TRT and alleviated mouse lung injury. Our results suggest that AIVs infection induce TRT byreducing SSU72 expression, thereby impairing host immune responses, a molecular mechanism acting through the NS1-SSU72-trans-TRT-STAT1/2 axis. Thus, restoration of SSU72 expression might be a potential strategy for preventing AIV pandemics. | Yan Zhao Fengming Huang Zhen Zou Yuhai Bi Yang Yang Cong Zhang Qiang Liu Daozhen Shang Yiwu Yan Xiangwu Ju Song Mei Peng Xie Xiao Li Mingyao Tian Shuguang Tan Huijun Lu Zongsheng Han Kangtai Liu Yuqing Zhang Junbo Liang Zhu Liang Qingchao Zhang Jiahui Chang William JLiu Cong Feng Tanshi Li Michael Q.Zhang Xiaoyue Wang George FGao Yingxia Liu Ningyi Jin Chengyu Jiang | 2022 | Cellular & Molecular Immunology2022，19，6： | 0 |
| 19 | Comparative transcriptomes reveal the disjunction adaptive strategy of Thuja species in East Asia and North America显示文摘The genus Thuja is ideal for investigating the genetic basis of the East Asia-North America disjunction.The biogeographical background of the genus is debatable and an adaptive strategy is lacking.Through the analysis and mining of comparative transcriptomes,species differentiation and positively selected genes(PSGs)were identified to provide information for understanding the environmental adaptation strategies of the genus Thuja.De novo assembly yielded 44,397-74,252 unigenes of the five Thuja species with contig N50length ranging from 1,559 to 1,724 bp.Annotations revealed a similar distribution of functional categories among them.Based on the phylogenetic trees constructed using the transcriptome data,T.sutchuenensis was divided first,followed by T.plicata and T.occidentalis.The final differentiation of T.koraiensis and T.standishii formed a clade.Enrichment analysis indicated that the PSGs of the North American Thuja species were involved in plant hormone signal transduction and carbon fixation of photosynthetic organisms pathways.The PSGs of East Asian Thuja were related to phenolic,alkaloid,and terpenoid synthesis,important stress-resistant genes and could increase plant resistance to external environmental stresses.This study discovered numerous aroma synthetic-related PSGs including terpene synthase(TPS)genes and lipid phosphate phosphatase 2(LPP2),associated with the synthetic aroma of T.sutchuenensis.Physiological indicators,such as the contents of soluble sugars,total chlorophyll,total phenolics,and total flavonoids were determined,which are consistent with the PSGs enrichment pathways associated with adaptive strategies in the five Thuja species.The results of this study provide an important basis for future studies on conservation genetics. | Ermei Chang Xue Liu Jiahui Chen Jingyi Sun Shaowei Yang Jianfeng Liu | 2023 | Journal of Forestry Research2023，34，6： | 0 |
| 20 | Eugenol targeting CrtM inhibits the biosynthesis of staphyloxanthin in Staphylococcus aureus显示文摘Staphylococcus aureus is a serious foodborne pathogen threatening food safety and public health.Especially the emergence of methicillin-resistant Staphylococcus aureus(MRSA)increased the difficulty of S.aureus treatment.Staphyloxanthin is a crucial virulence factor of S.aureus.Blocking staphyloxanthin production could help the host immune system counteract the invading S.aureus cells.In this study,we first screened for staphyloxanthin inhibitors using a virtual screening method.The outcome of the virtual screening method resulted in the identification of eugenol(300μg/mL),which significantly inhibits the staphyloxanthin production in S.aureus ATCC 29213,S.aureus Newman,MRSA ATCC 43300 and MRSA ATCC BAA1717by 84.2%,63.5%,68.1%,and 79.5%,respectively.The outcome of the growth curve assay,field-emission scanning electron,and confocal laser scanning microscopy analyses confirmed that eugenol at the test concentration did not affect the morphology and growth of S.aureus.Moreover,the survival rate of S.aureus ATCC 29213 and MRSA ATCC 43300 under H_(2)O_(2) pressure decreased to 51.9%and 45.5%in the presence of eugenol,respectively.The quantitative RT-PCR and molecular simulation studies revealed that eugenol targets staphyloxanthin biosynthesis by downregulating the transcription of the crtM gene and inhibiting the activity of the CrtM enzyme.Taken together,we first determined that eugenol was a prominent compound for staphyloxanthin inhibitor to combat S.aureus especially MRSA infections. | Jiang Chang Bo Chen Zeqian Du Bowen Zhao Jiahui Li Ziyi Li Kannappan Arunachalam Ting Shi Dongqing Wei Chunlei Shi | 2024 | Food Science and Human Wellness2024，13，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员