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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Transmission Dynamics of an Outbreak of the COVID-19 Delta Variant B.1.617.2——Guangdong Province,China,May-June 2021显示文摘On May 21,2021,a local case of coronavirus disease 2019(COVID-19)was confirmed in a 75-year-old woman(experienced onset of symptoms on May 18)in Liwan District,Guangzhou City,Guangdong Province,China.The number of infections has increased in the following 10 days and led to 5 generations of transmission. | Meng Zhang Jianpeng Xiao Aiping Deng Yingtao Zhang Yali Zhuang Ting Hu Jiansen Li Hongwei Tu Bosheng Li Yan Zhou Jun Yuan Lei Luo Zimian Liang Youzhi Huang Guoqiang Ye Mingwei Cai Gongli Li Bo Yang Bin Xu Ximing Huang Yazun Cui Dongsheng Ren Yanping Zhang Min Kang Yan Li | 2021 | China CDC weekly2021，3，27： | 51 |
| 3 | The Structure of Ore-controlling Strain and Stress Fields in the Shangzhuang Gold Deposit in Shandong Province,China显示文摘Shangzhuang 改变岩石的类型金牌矿石存款位于中间的片断并且在 Jiaodong 金省的西北的部分由 Wang'ershan 差错地区控制了,中国。在 Shangzhuang 存款的变丑进化,紧张的结构和压力地和它的控制矿石的效果在这份报纸被讨论。变丑进化主要经历了四个阶段,这被揭示:早可锻的变丑,第二 NE 惹人注目水平简单砍,第三 NE 惹人注目的压缩砍和最后的 NW 惹人注目的压缩。矿化作用发生在在的第三个阶段期间最大的主要压力逐渐地从 NE 的 transited 到 NW。 3-D 压力地的数字模拟证明在最大的主要压力是NE惹人注目的条件上,在差错地区的破裂发展被赞成,当时当最大的主要压力是NW惹人注目的时,差错地区是相对 extensional 和它对形成矿石的液体的流入和炮兵阵地合适。压缩砍紧张地被类型结构在矿化作用期间描绘,积极的花结构,等等。Orebodies 等距离地主要位于 dilatational 空格,它在不可分的有压缩性的情形是分布式的。并且在差错态度变化的地方, dilatational 空格被开发或砍联合系统发展。在全面压缩砍应力地里,紧张地在多重规模忍受自我类似,包括 orebody,矿石存款和 orefield。结构的自我类似在各种各样的规模在一样的规模和破裂的类似的形状包括破裂的 subequidistant 分发。由于特殊地质的结构,然而, orebodies 主要在 Shangzhuang 存款位于挂的墙它与在 Jiaodong 金省的大多数存款不同。在存款的控制矿石的压力和紧张地的分析为寻求的沉积物提供一个重要基础。 | DENG Jun WANG Qingfei YANG Liqiang ZHOU Lei GONG Qingjie YUAN Wanming XU Hao GUO Chunying LIU Xiangwei | 2008 | Acta Geologica Sinica(English Edition)2008，82，4： | 41 |
| 4 | An Interdisciplinary Nutrition Support Team Improves Clinical and Hospitalized Outcomes of Esophageal Cancer Patients with Concurrent Chemoradiotherapy显示文摘 | Ming-Hua Cong Shu-Luan Li Guo-Wei Cheng Jin-Ying Liu Chen-Xin Song Ying-Bing Deng Wei-Hu Shang Di Yang Xue-Hui Liu Wei-Wei Liu Shi-Yan Lu Lei Yu | 2015 | Chinese Medical Journal2015，，22： | 41 |
| 5 | Risk assessment and validation of flood disaster based on fuzzy mathematics显示文摘Floods often take place around rivers and plains,which indicates a higher risk of flooding in these areas.This paper adopts fuzzy comprehensive assessment(FCA),simple fuzzy classification(SFC),and the fuzzy similarity method(FSM) to assess flood disaster risk in Kelantan,Malaysia.Validation data,such as the flooded area,paddy area,urban area,residential area,and refuges,were overlaid to validate and analyze the accuracy of flood disaster risk.The results show that(1) 70-75% of flooded areas lie within the higher and high-est risk zones,which shows an effective assessment accuracy;(2) paddy,built-up,and residential areas concentrated in the higher and highest risk zones are more likely to be destroyed by flood disasters;(3) 200-225 refuges in the higher and highest risk zones account for around 50% of all refuges,which means that more refuges should be built in the higher and highest risk zones to meet the accommodation requirement;(4) three methods proved to be feasible and effective in evaluating flood disaster risk,among which FCA is more suitable for the study area than the two other methods. | Weiguo Jiang Lei Deng Luyao Chen Jianjun Wu Jing Li | 2009 | Progress in Natural Science:Materials International2009，19，10： | 35 |
| 6 | Mesozoic contraction deformation in the Yanshan and northern Taihang mountains and its implications to the destruction of the North China Craton显示文摘Mesozoic contraction deformation in the Yanshan and Taihang mountains is characterized by basement-involved thrust tectonics,basement-cored buckling anticlines and ductile thrust and nappe tectonics.Most of these deformations are orientated west-east,west-northwest and northeast to north-northeast.The contraction deformations began in the Permian,continued through the Triassic and Jurassic and terminated in the Early Cretaceous,and constitute an important part of the destruction of the North China Craton.It is estimated,from balanced cross-section reconstructions,that the north-south shortening of the central part of the Yanshan belt before 135 Ma was around 38%.The initial crust thickness,pre-dating the major contraction deformation in late Paleozoic and early Mesozoic,was estimated to be around 35 km based on paleogeographic characteristics.Assuming that the inferred depth of ductile thrusting deformation,20-25 km,was the crust thickness involved in the contraction deformation,and also assuming that the N-S contraction deformation was accommodated by vertical crust thickening,the thickness of the crust after the contraction deformation was expected to be around 47-50 km.This was the approximate crust thickness required for the eclogitization of the lower crust for delamination.The gravity potential accumulated by the isostatic uplift of the thickened crust,together with the decrease in crustal strength caused by the coeval magmatisms associated with the contraction deformation,led to the subsequent extensional collapse of the middle and upper crust although the regional stress regime associated with the plate interactions remained constant.It is inferred that the Mesozoic contraction deformations in the Yanshan and Taihang mountains were not only a significant tectonic process contributing to the destruction of the craton in middle and upper crust but also stimulated delamination at a deep level and the extension of the shallow crust.In other words,both the suspected delamination of the lower crust and upper mantle and the well constrained extension deformations of the shallow crust in the eastern North China Craton during the late Mesozoic are a consequence of crust thickening due to previous contractions.Extensional deformations could be expected to occur independently in the shallow crust,and are not necessarily associated with or responding to delamination at a deep level. | ZHANG ChangHou LI ChengMing DENG HongLing LIU Yang LIU Lei WEI Bo LI HanBin LIU Zi | 2011 | Science China Earth Sciences2011，54，6： | 40 |
| 7 | Analysis of factors associated with disease outcomes in hospitalized patients with 2019 novel coronavirus disease显示文摘Background Since early December 2019,the 2019 novel coronavirus disease(COVID-19)has caused pneumonia epidemic in Wuhan,Hubei province of China.This study aimed to investigate the factors affecting the progression of pneumonia in COVID-19 patients.Associated results will be used to evaluate the prognosis and to find the optimal treatment regimens for COVID-19 pneumonia.Methods Patients tested positive for the COVID-19 based on nucleic acid detection were included in this study.Patients were admitted to 3 tertiary hospitals in Wuhan between December 30,2019,and January 15,2020.Individual data,laboratory indices,imaging characteristics,and clinical data were collected,and statistical analysis was performed.Based on clinical typing results,the patients were divided into a progression group or an improvement/stabilization group.Continuous variables were analyzed using independent samples t-test or Mann-Whitney U test.Categorical variables were analyzed using Chi-squared test or Fisher’s exact test.Logistic regression analysis was performed to explore the risk factors for disease progression.Results Seventy-eight patients with COVID-19-induced pneumonia met the inclusion criteria and were included in this study.Efficacy evaluation at 2 weeks after hospitalization indicated that 11 patients(14.1%)had deteriorated,and 67 patients(85.9%)had improved/stabilized.The patients in the progression group were significantly older than those in the disease improvement/stabilization group(66[51,70]vs.37[32,41]years,U=4.932,P=0.001).The progression group had a significantly higher proportion of patients with a history of smoking than the improvement/stabilization group(27.3%vs.3.0%,χ^2=9.291,P=0.018).For all the 78 patients,fever was the most common initial symptom,and the maximum body temperature at admission was significantly higher in the progression group than in the improvement/stabilization group(38.2[37.8,38.6]vs.37.5[37.0,38.4]°C,U=2.057,P=0.027).Moreover,the proportion of patients with respiratory failure(54.5%vs.20.9%,χ^2=5.611,P=0.028)and respiratory rate(34[18,48]vs.24[16,60]breaths/min,U=4.030,P=0.004)were significantly higher in the progression group than in the improvement/stabilization group.C-reactive protein was significantly elevated in the progression group compared to the improvement/stabilization group(38.9[14.3,64.8]vs.10.6[1.9,33.1]mg/L,U=1.315,P=0.024).Albumin was significantly lower in the progression group than in the improvement/stabilization group(36.62±6.60 vs.41.27±4.55 g/L,U=2.843,P=0.006).Patients in the progression group were more likely to receive high-level respiratory support than in the improvement/stabilization group(χ^2=16.01,P=0.001).Multivariate logistic analysis indicated that age(odds ratio[OR],8.546;95%confidence interval[CI]:1.628-44.864;P=0.011),history of smoking(OR,14.285;95%CI:1.577-25.000;P=0.018),maximum body temperature at admission(OR,8.999;95%CI:1.036-78.147,P=0.046),respiratory failure(OR,8.772,95%CI:1.942-40.000;P=0.016),albumin(OR,7.353,95%CI:1.098-50.000;P=0.003),and C-reactive protein(OR,10.530;95%CI:1.224-34.701,P=0.028)were risk factors for disease progression.Conclusions Several factors that led to the progression of COVID-19 pneumonia were identified,including age,history of smoking,maximum body temperature at admission,respiratory failure,albumin,and C-reactive protein.These results can be used to further enhance the ability of management of COVID-19 pneumonia. | Wei Liu Zhao-Wu Tao Lei Wang Ming-Li Yuan Kui Liu Ling Zhou Shuang Wei Yan Deng Jing Liu Hui-Guo Liu Ming Yang Yi Hu | 2020 | Chinese Medical Journal2020，，9： | 31 |
| 8 | Clinical characteristics of fatal and recovered cases of coronavirus disease 2019 in Wuhan,China:a retrospective study显示文摘Background:The 2019 novel coronavirus has caused the outbreak of the acute respiratory disease in Wuhan,Hubei Province of China since December 2019.This study was performed to analyze the clinical characteristics of patients who succumbed to and who recovered from 2019 novel coronavirus disease(COVID-19).Methods:Clinical data were collected from two tertiary hospitals in Wuhan.A retrospective investigation was conducted to analyze the clinical characteristics of fatal cases of COVID-19(death group)and we compare them with recovered patients(recovered group).Continuous variables were analyzed using the Mann-WhitneyU test.Categorical variables were analyzed byχ2 test or Fisher exact test as appropriate.Results:Our study enrolled 109 COVID-19 patients who died during hospitalization and 116 recovered patients.The median age of the death group was older than the recovered group(69[62,74]vs.40[33,57]years,Z=9.738,P<0.001).More patients in the death group had underlying diseases(72.5%vs.41.4%,χ2=22.105,P<0.001).Patients in the death group had a significantly longer time of illness onset to hospitalization(10.0[6.5,12.0]vs.7.0[5.0,10.0]days,Z=3.216,P=0.001).On admission,the proportions of patients with symptoms of dyspnea(70.6%vs.19.0%,χ2=60.905,P<0.001)and expectoration(32.1%vs.12.1%,χ2=13.250,P<0.001)were significantly higher in the death group.The blood oxygen saturation was significantly lower in the death group(85[77,91]%vs.97[95,98]%,Z=10.625,P<0.001).The white blood cell(WBC)in death group was significantly higher on admission(7.23[4.87,11.17]vs.4.52[3.62,5.88]×109/L,Z=7.618,P<0.001).Patients in the death group exhibited significantly lower lymphocyte count(0.63[0.40,0.79]vs.1.00[0.72,1.27]×109/L,Z=8.037,P<0.001)and lymphocyte percentage(7.10[4.45,12.73]%vs.23.50[15.27,31.25]%,Z=10.315,P<0.001)on admission,and the lymphocyte percentage continued to decrease during hospitalization(7.10[4.45,12.73]%vs.2.91[1.79,6.13]%,Z=5.242,P<0.001).Alanine transaminase(22.00[15.00,34.00]vs.18.70[13.00,30.38]U/L,Z=2.592,P=0.010),aspartate transaminase(34.00[27.00,47.00]vs.22.00[17.65,31.75]U/L,Z=7.308,P<0.001),and creatinine levels(89.00[72.00,133.50]vs.65.00[54.60,78.75]μmol/L,Z=6.478,P<0.001)were significantly higher in the death group than those in the recovered group.C-reactive protein(CRP)levels were also significantly higher in the death group on admission(109.25[35.00,170.28]vs.3.22[1.04,21.80]mg/L,Z=10.206,P<0.001)and showed no significant improvement after treatment(109.25[35.00,170.28]vs.81.60[27.23,179.08]mg/L,Z=1.219,P=0.233).The patients in the death group had more complications such as acute respiratory distress syndrome(ARDS)(89.9%vs.8.6%,χ2=148.105,P<0.001),acute cardiac injury(59.6%vs.0.9%,χ2=93.222,P<0.001),acute kidney injury(18.3%vs.0%,χ2=23.257,P<0.001),shock(11.9%vs.0%,χ2=14.618,P<0.001),and disseminated intravascular coagulation(DIC)(6.4%vs.0%,χ2=7.655,P=0.006).Conclusions:Compared to the recovered group,more patients in the death group exhibited characteristics of advanced age,pre-existing comorbidities,dyspnea,oxygen saturation decrease,increased WBC count,decreased lymphocytes,and elevated CRP levels.More patients in the death group had complications such as ARDS,acute cardiac injury,acute kidney injury,shock,and DIC. | Yan Deng Wei Liu Kui Liu Yuan-Yuan Fang Jin Shang Ling Zhou Ke Wang Fan Leng Shuang Wei Lei Chen Hui-Guo Liu | 2020 | Chinese Medical Journal2020，，11： | 29 |
| 9 | A Genome-Wide Transcription Analysis Reveals a Close Correlation of Promoter INDEL Polymorphism and Heterotic Gene Expression in Rice Hybrids显示文摘杂种优势,或混合活力,指二个生来的变化的混合子孙在展出提高的生长或农学的性能的现象。尽管研究的世纪长的历史关于杂种优势的基因基础产生了几个假设,位于杂种优势和 heterotic 基因表示下面的分子的机制留下逃犯。这里,我们在米饭报导二个 heterotic 十字的染色体宽的基因表示分析,利用它的充分定序的染色体。约 79% 基因是从 heterotic 十字的二个集合在幼苗射击表示的差别,包括许多抄写因素基因,并且展出了基因行动的多重模式。在生来的父母之间的 ortholog 基因的通常认为的倡导者区域的比较揭示了广泛的顺序变化,特别地小的插入 / 删除(INDEL ) ,许多哪个在通常认为的 cis 规章的元素的形成 / 混乱的结果。一起,这些结果建议那在抄写因素和多态的倡导者的表示之间的组合相互影响在混血儿的 cis 规章的元素是位于 heterotic 基因行动下面的一嘴巧的分子的机制并且这样在米饭的杂种优势。 | Hui-Yong Zhang Hang He Liang-Bi Chen Lei Li Man-Zhong Liang Xiang-Feng Wang Xi-Gang Liu Guang-Ming He Run-Sheng Chen Li-Geng Ma Xing Wang Deng | 2008 | Molecular Plant2008，1，5： | 25 |
| 10 | PTEN-L is a novel protein phosphatase for ubiquitin dephosphorylation to inhibit PINK1-Parkin-mediated mitophagy显示文摘Mitophagy 是为损坏线粒体的特定的消除的选择 autophagy 的一种重要类型。(PINK1 )导致 PTEN 的通常认为的 kinase 蛋白质 1 催化 ubiquitin (Ub ) 的 phosphorylation 在 PINK1-Parkin-mediated mitophagy 的发作起一个关键作用。(PTEN-L ) 磷酸酶和 tensin 相当或相同的事物(PTEN ) 长是 PTEN 的最新识别的 isoform,与到它的 N 终点的 173 氨基酸的增加。这里,我们报导 PTEN-L 是经由它对 phosphorylated ubiquitin 的蛋白质磷酸酶活动的 mitophagy 的一个新奇否定管理者。我们发现 PTEN-L 在外部 mitochondrial 膜(OMM ) 本地化,而 PTEN-L 的删除支持, PTEN-L 的 overexpression 禁止, mitophagy 由各种各样的损坏线粒体的代理人导致了。机械学地, PTEN-L 能够有效地阻止 Parkin mitochondrial translocation,减少 Parkin phosphorylation,维持它的关上的不活跃的符合构造,并且禁止它的 E3 ligase 活动。更重要地, PTEN-L 在 vivo 减少 phosphorylated ubiquitin (pSer65-Ub ) 的水平,并且在 vitro,磷酸酶试金经由它的蛋白质磷酸酶活动证实那 PTEN-L dephosphorylates pSer65-Ub,独立于它的类脂化合物磷酸酶功能。一起拿,我们的调查结果为 ubiquitin 作为蛋白质磷酸酶表明 PTEN-L 的新奇功能,它抵抗在 mitophagy 正式就职和 mitophagy 的最终的抑制导致前馈控制机制的阻塞的调停 PINK1 的 ubiquitin phosphorylation。因此,理解 PTEN-L 的这新奇功能在控制 mitophagy 的分子的难题提供一关键错过片,在包括象 Parkinsons 那样的 neurodegenerative 混乱的许多重要人的疾病的一个批评过程疾病。 | Liming Wang Yik-Lam Cho Yancheng Tang Jigang Wang Jung-Eun Park Yajun Wu Chunxin Wang Yan Tong Ritu Chawla Jianbin Zhang Yin Shi Shuo Deng Guang Lu Yihua Wu Hayden Weng-Siong Tan Pornteera Pawijit Grace Gui-Yin Lim Hui-Ying Chan Jingzi Zhang Lei Fang Hanry Yu Yih-Cherng Liou Mallilankaraman Karthik Boon-Huat Bay Kah-Leong Lim Siu-Kwan Sze Celestial T. Yap Han-Ming Shen | 2018 | Cell Research2018，28，8： | 25 |
| 11 | Effects of the grain-for-green program on soil erosion in China显示文摘严重土壤侵蚀是在中国的一个严重环境问题。在 1999,中国政府实现了 Grain-for-Green 节目(此处叫作节目) ,到帮助控制的生态的重建的一个很好圆的节目这个国家的土壤侵蚀问题。这学习份量上从 11 个中国河系统用数据分析了程序的影响在土壤侵蚀上评估程序的效果。结果证明流量减少了,因为变换农田的林地的区域的增加,土壤侵蚀显著地减少了。当两次比较时期, 2003-2007 和 1998-2002 时,流量和土壤侵蚀被 18% 和 45.4% 分别地减少。因此,当生产生态的好处时,程序有控制土壤侵蚀的效果。 | Lei DENG Zhou-ping SHANGGUAN Rui LI | 2012 | International Journal of Sediment Research2012，27，1： | 24 |
| 12 | Strength reduction and step-loading finite element approaches in geotechnical engineering显示文摘The finite element limit analysis method has the advantages of both numerical and traditional limit equilibrium techniques and it is particularly useful to geotechnical engineering.This method has been developed in China,following well-accepted international procedures,to enhance understanding of stability issues in a number of geotechnical settings.Great advancements have been made in basic theory,the improvement of computational precision,and the broadening of practical applications.This paper presents the results of research on(1) the efficient design of embedded anti-slide piles,(2) the stability analysis of reservoir slopes with strength reduction theory,and(3) the determination of the ultimate bearing capacity of foundations using step-loading FEM(overloading).These three applications are evidence of the design improvements and benefits made possible in geotechnical engineering by finite element modeling. | Yingren Zheng Xiaosong Tang Shangyi Zhao Chujian Deng Wenjie Lei | 2009 | Journal of Rock Mechanics and Geotechnical Engineering2009，1，1： | 23 |
| 13 | Crystal structure and biochemical analyses reveal Beclin 1 as a novel membrane binding protein显示文摘Beclin 1 基因是 haplo 不够的肿瘤 suppressor 并且在 autophagy 起一个必要作用。然而, Beclin 1 由工作的分子的机制仍然保持大部分未知。这里,我们在 1.6 Å 报导 Beclin 1 的 evolutionarily 保存的领域(ECD ) 的水晶结构;分辨率。Beclin 1 ECD 展览一以前 unreported 褶层,与在中央轴附近相称性地安排的三次结构的重复。Beclin 1 ECD 定义膜绑定领域的一个新奇的类,与为与 cardiolipin 充实的类脂化合物膜的强壮的偏爱。在 Beclin 1 ECD 的一个表面环的尖端,包括三芳香的氨基酸,与类脂化合物膜充当一根恐水病的手指到伙伴,因而导致膜和 liposomes 的变丑。这些芳香的残余的变化使 Beclin 1 变为不能稳定地在 vitro 与类脂化合物膜联系并且不能充分在 vivo 在 Beclin 1-knockdown 房间救 autophagy。这些观察为译解形成一个重要框架 Beclin 1 的生物功能。 | Weljlao Huang Wooyoung Chol Wanqiu Hu Na Mi Qiang Guo Meisheng Ma Mei Liu Yuan Tian Peilong Lu Feng-Liang Wang Haiteng Deng Lei Liu Ning Gao Li Yu Yigong Shi | 2012 | Cell Research2012，22，3： | 21 |
| 14 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 15 | Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca^(2+)-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats显示文摘AIM: To investigate the effect of Chaiqinchengqi decoction (CQCQD) on sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) mRNA expression of pancreatic tissues in acute pancreatitis (AP) rats. METHODS: Thirty Sprague-Dawley (SD) rats were randomized into control group, AP group and CQCQD group (n = 3 × 10). The rats in the CQCQD group were intragastrically administered with CQCQD (10 mL/kg every 2 h) after induction of AP by intraperitoneal injection of caerulein (50 μg/kg.h × 5) within 4 h. At 6 h after the induction of AP model, pancreatic tissues were collected for the pathological observation, mRNA extraction for determination of SERCA1 and SERCA2 mRNA expression or pancreatic acinar cell isolation for measurement of fluorescence intensity (FI) of intracellular calcium ion concentration [Ca2+]i. RESULTS: There was no expression of pancreatic SERCA1 mRNA in the control group and the AP group. The expression of pancreatic SERCA2 mRNA in the AP group was down-regulated (expression ratio = 0.536; P = 0.001) compared with the control group, while that in the CQCQD group was up-regulated (expression ratio= 2.00; P = 0.012) compared with AP group. The FI of intracellular [Ca2+] of pancreatic acinar cells in the AP group (138.2 ± 23.1) was higher than the C group (111.0 ± 18.4) and the CQCQD group (118.7 ± 15.2 ) (P < 0.05) and the pancreatic pathological score in the CQCQD group was lower than that in the AP group (5.7 ± 1.9 vs 9.2 ± 2.7, P < 0.05).CONCLUSION: CQCQD can up-regulate the expression of SERCA2 mRNA of pancreatic tissues, reduce intracellular calcium overload and relieve pancreatic tissue lesions. | Ping Xue Li-Hui Deng Zhao-Da Zhang Xiao-Nan Yang Qing Xia Da-Kai Xiang Lei Huang Mei-Hua Wan | 2008 | World Journal of Gastroenterology2008，14，15： | 20 |
| 16 | Portosplenomesenteric vein thrombosis in patients with early-stage severe acute pancreatitis显示文摘AIM To investigate the incidence and risk factors of portosplenomesenteric vein thrombosis(PSMVT) in the early stage of severe acute pancreatitis(SAP).METHODS Patients with SAP in a tertiary care setting from January 2014 to December 2016 were retrospectively reviewed. All contrast-enhanced computed tomography(CT) studies were reassessed and reviewed. Clinical outcome measures were compared between SAP patients with and without PSMVT in the early stage of the disease. Univariate and multivariate logistic regression analyses were sequentially performed to assess potential risk factors for the development of PSMVT in SAP patients. A receiver operating characteristic(ROC) curve was generated for the qualifying independent risk factors.RESULTS Twenty-five of the one hundred and forty(17.86%) SAP patients developed PSMVT 6.19 ± 2.43 d after acute pancreatitis(AP) onset. PSMVT was confirmed by contrast-enhanced CT. Multivariate stepwise logistic regression analyses showed that Balthazar's CT severity index(CTSI) scores [odds ratio(OR): 2.742; 95% confidence interval(CI): 1.664-4.519; P = 0.000], hypoalbuminemia(serum albumin level < 25 g/L)(OR: 32.573; 95%CI: 2.711-391.353; P = 0.006) and gastrointestinal wall thickening(OR: 4.367, 95%CI: 1.218-15.658; P = 0.024) were independent risk factors for PSMVT developed in patients with SAP. The area under the ROC curve for Balthazar's CTSI scores was 0.777(P = 0.000), the sensitivity was 52%, and the specificity was 93% at a cut-off value of 5.5.CONCLUSION High Balthazar's CTSI scores, hypoalbuminemia and gastrointestinal wall thickening are independent risk factors for PSMVT developed in the early stage of SAP. | Ling Ding Feng Deng Chen Yu Wen-Hua He Liang Xia Mi Zhou Xin Huang Yu-Peng Lei Xiao-Jiang Zhou Yin Zhu Nong-Hua Lu | 2018 | World Journal of Gastroenterology2018，24，35： | 20 |
| 17 | Impaired balance of T helper 17/T regulatory cells in carbon tetrachloride-induced liver fibrosis in mice显示文摘AIM:To investigate the effect of T helper(Th)17/T regulatory(Treg)cells on hepatic fibrosis in mice and its possible mechanism.METHODS:Hepatic fibrosis was induced by intraperitoneal injection of carbon tetrachloride.Hepatic pathological changes were observed by hematoxylin and eosin staining;the protein levels of interleukin(IL)-6,transforming growth factor(TGF)-βandα-smooth muscle actin(SMA)in liver tissue were determined by Western blotting;and the frequency of Th17 and Treg cells in the liver was estimated by flow cytometry.In addition,hepatic stellate cells were isolated from healthy mouse liver and co-cultured with Th17 or Treg cells.Immunofluorescence staining and Western blotting were performed to determine the change in HSC activation.RESULTS:In the model group,there were different degrees of fibroplasia,degeneration and necrosis.The protein levels of IL-6,TGF-βandα-SMA in liver tissue were significantly higher than those in the control group at 12 wk(P<0.05).Compared with the control group,the frequency of Th17 cells in the model group was increased but the frequency of Treg cells decreased gradually.Furthermore,at 4,8 and 12 wk,there were significant differences in the number of Th17 cells(0.52%±0.16%,1.46%±0.24%,and2.60%±0.41%,respectively,P<0.05)and Treg cells(2.99%±0.40%,2.16%±0.50%,and 1.49%±0.34%,respectively,P<0.05).In vitro,Th17 cells promoted,whereas Treg cells inhibited the expression ofα-SMA,both in a dose-dependent manner,compared with the control group.CONCLUSION:Th17/Treg imbalance exists in mice with liver fibrosis,which potentially promotes liver fibrosis via HSC activation. | Xiao-Fei Sun Lei Gu Wen-Sheng Deng Qing Xu | 2014 | World Journal of Gastroenterology2014，20，8： | 20 |
| 18 | Plasma miR-216a as a potential marker of pancreatic injury in a rat model of acute pancreatitis显示文摘AIM:To study the potential value and specificity of plasma miR-216a as a marker for pancreatic injury.METHODS:Two rat models were applied in this article:L-arginine-induced acute pancreatitis was used as one model to explore the potential value of plasma miR-216a for detection of pancreatic injury;nonlethal sepsis induced in rats by single puncture cecal ligation and puncture(CLP)was used as the other model to evaluate the specificity of plasma miR-216a compared with twocommonly used markers(amylase and lipase)for acute pancreatitis.Plasmas were sampled from rats at indicated time points and total RNA was isolated.Real-Time Quantitative reverse transcriptase-polymerase chain reaction was used to quantify miR-216a in plasmas.RESULTS:In the acute pancreatitis model,among five time points at which plasmas were sampled,miR-216a concentrations were significantly elevated 24 h after arginine administration and remained significantly increased until 48 h after operation(compared with 0 h time point,P<0.01,Kruskal-Wallis Test).In the CLP model,plasma amylase and lipase,two commonly used biomarkers for acute pancreatitis,were significantly elevated 24 h after operation(compared with 0 h time point,P<0.01 and 0.05 respectively,Pairwise Bonferroni corrected ttests),while miR-216a remained undetectable among four tested time points.CONCLUSION:Our article showed for the first time that plasma miR-216a might serve as a candidate marker of pancreatic injury with novel specificity. | Xiang-Yu Kong,Yi-Qi Du,Lei Li,Xiao-Hua Man,Yan-Fang Gong,Li-Ning Xiao,Yong-Zhi Zheng,Shang-Xin Deng,Jun-Jun Gu,Zhao-Shen Li,Department of Gastroenterology,Changhai Hospital,Second Military Medical University,Shang-hai 200433,China Jian-Qiang Liu,Department of Gastroenterology,Fuzhou General Hospital of Nanjing Military Command,Fuzhou 350025,Fujian Province,China Guo-Kun Wang,Jia-Qi Zhu,Department of Cardiology,Changhai Hospital,Second Military Medical University,Shang-hai 200433,China | 2010 | World Journal of Gastroenterology2010，16，36： | 19 |
| 19 | In vitro culture and differentiation of rat embryonic midbrain-derived neural stem cells显示文摘BACKGROUND:Midbrain-derived neural stem cells (mNSCs) can differentiate into functional mature dopaminergic neurons. The mNSCs are considered the ideal choice for cell therapy of Parkinson’s disease. OBJECTIVE: To isolate rat embryonic mNSCs and to observe the differentiation characteristics of mNSCs induced by cell growth-promoting factors. DESIGN,TIME AND SETTING: An in vitro cell culture study based on the molecular biology of nerve cells was carried out at the Institute of Clinical Medicine,China-Japan Friendship Hospital (China) from March to November 2007. MATERIALS: Sprague Dawley rats at embryonic day 14 were used in this study. Nestin antibody,β-Ⅲ tubulin antibody,glial fibrillary acidic protein (GFAP) antibody and cyclic nucleotide 3’-phosphohydrolase (CNPase) antibody were provided by Abcam; DMEM/F12 medium and N2 supplement were provided by In-vitrogen; epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF2) were provided by R&D Sys-tems. METHODS: The ventral mesencephalon was dissected from embryonic day 14 rat embryos. By trypsin di-gestion and mechanical separation,the brain tissue was triturated into a fine single-cell suspension. The cells were cultured in 5 mL serum-free medium containing DMEM/F12,1% N2 supplement,20 ng/mL EGF and FGF2. The mNSCs at the third generation were coated with 10 μg/mL polylysine and induced to differenti-ate in the DMEM/F12 supplemented with 1% fetal bovine serum and 1% N2. MAIN OUTCOME MEASURES: The neural spheres of the third passage were identified by nestin im-munofluorescence; at the same time,the cells were induced to differentiate,and the types of differentiated cell were identified by immunofluorescence for βⅢ tubulin,GFAP and CNPase. RESULTS: Seven days after primary culture,a great many neurospheres could be obtained by successive pasage. Immunofluorescence assays showed that the neurospheres were nestin positive,and after differentia-tion,the cells expressed GFAP,CNPase and β-Ⅲ-tubulin. CONCLUSION: Embryonic day 14 rat mNSCs can differentiate into neuron-like cells and glial cells fol-lowing induction by EGF,FGF2 and N2 additive. | Xingli Deng Ruen Liu Zhongtang Feng Jing Guo Wu Wang Deqiang Lei Hongyan Li Zhihua Chen | 2008 | Neural Regeneration Research2008，3，11： | 19 |
| 20 | Advanced Space-based Solar Observatory(ASO-S):an overview显示文摘The Advanced Space-based Solar Observatory(ASO-S)is a mission proposed for the 25 th solar maximum by the Chinese solar community.The scientific objectives are to study the relationships between the solar magnetic field,solar flares and coronal mass ejections(CMEs).Three payloads are deployed:the Full-disk vector Magneto Graph(FMG),the Lyman-αSolar Telescope(LST)and the Hard X-ray Imager(HXI).ASO-S will perform the first simultaneous observations of the photospheric vector magnetic field,non-thermal imaging of solar flares,and the initiation and early propagation of CMEs on a single platform.ASO-S is scheduled to be launched into a 720 km Sun-synchronous orbit in 2022.This paper presents an overview of the mission till the end of Phase-B and the beginning of Phase-C. | Wei-Qun Gan Cheng Zhu Yuan-Yong Deng Hui Li Yang Su Hai-Ying Zhang Bo Chen Zhe Zhang Jian Wu Lei Deng Yu Huang Jian-Feng Yang Ji-Jun Cui Jin Chang Chi Wang Ji Wu Zeng-Shan Yin Wen Chen Cheng Fang Yi-Hua Yan Jun Lin Wei-Ming Xiong Bin Chen Hai-Chao Bao Cai-Xia Cao Yan-Ping Bai Tao Wang Bing-Long Chen Xin-Yu Li Ye Zhang Li Feng Jiang-Tao Su Ying Li Wei Chen You-Ping Li Ying-Na Su Hai-Yan Wu Mei Gu Lei Huang Xue-Jun Tang | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 17 |
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