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| 1 | Recent Progress Using High-throughput Sequencing Technologies in Plant Molecular Breeding显示文摘High-throughput sequencing is a revolutionary technological innovation in DNA sequencing.This technology has an ultra-low cost per base of sequencing and an overwhelmingly high data output.High-throughput sequencing has brought novel research methods and solutions to the research fields of genomics and post-genomics.Furthermore,this technology is leading to a new molecular breeding revolution that has landmark significance for scientific research and enables us to launch multi-level,multi-faceted,and multi-extent studies in the fields of crop genetics,genomics,and crop breeding.In this paper,we review progress in the application of high-throughput sequencing technologies to plant molecular breeding studies. | Qiang Gao Guidong Yue Wenqi Li Junyi Wang Jiaohui Xu Ye Yin | 2012 | Journal of Integrative Plant Biology2012,54,4: | 15 |
| 2 | Expression of ipt gene driven by tomato fruit specific promoter and its effects on fruit development of tomato显示文摘Fruit specific promoter (2A12) from Lycopersi-com esculentum and cDNA of isopentenyl-transferase (ipt) from Ti plasmid of Agrobacterium tumerfaciens C58 were cloned by PCR procedure respectively. Two plant expression vectors with 2A12/gus or 2A12/ipt were respectively constructed. These two chimeric genes were transferred into tomato by Agrobacterium mediated procedure. The results of Southern hybridization showed that the fusion genes had been integrated into tomatoes. The result of gus histochemi-cal staining showed that 2A12 had high fruit specific expressive capability in transgenic tomato. The ipt expression resulted in accumulation of high level of cytokinins (CTKs) in fruit lead to developmental changes in fruits and seeds. The fruit of ipt transformed tomato had the hyperplastic placenta with very few seeds or even seedless. The shelf life of transgenic fruits elongated for 1-2 weeks. The ratio of fruit set, the dry weight of fruit and the crude protein content in fruit were increased, while | Zichao Mao Qiuju Yu Wei Zhen Junyi Guo Yuanlei Hu Yin Gao Zhongping Lin | 2002 | Chinese Science Bulletin2002,47,11: | 6 |
| 3 | Simplified unscented particle filter for nonlinear/non-Gaussian Bayesian estimation显示文摘Particle filters have been widely used in nonlinear/nonGaussian Bayesian state estimation problems.However,efficient distribution of the limited number of particles in state space remains a critical issue in designing a particle filter.A simplified unscented particle filter(SUPF) is presented,where particles are drawn partly from the transition prior density(TPD) and partly from the Gaussian approximate posterior density(GAPD) obtained by a unscented Kalman filter.The ratio of the number of particles drawn from TPD to the number of particles drawn from GAPD is adaptively determined by the maximum likelihood ratio(MLR).The MLR is defined to measure how well the particles,drawn from the TPD,match the likelihood model.It is shown that the particle set generated by this sampling strategy is more close to the significant region in state space and tends to yield more accurate results.Simulation results demonstrate that the versatility and estimation accuracy of SUPF exceed that of standard particle filter,extended Kalman particle filter and unscented particle filter. | Junyi Zuo Yingna Jia Quanxue Gao | 2013 | Journal of Systems Engineering and Electronics2013,24,3: | 6 |
| 4 | A new distinct geminivirus causes soybean stay-green disease显示文摘Dear Editor,Plant viruses make up almost half of the plant disease-causing pathogens,affecting crop yields and the global economy(Savary et al.,2019).Soybean(Glycine max)is one of the most valuable legume crops in the world,supplying 25%of the global edible oil and two-thirds of the global concentrated protein for livestock feeding.Recently,the outbreak of soybean stay-green syndrome with delayed leaf senescence(stay-green),flat pods,and increased number of abnormal seeds has swept the soybean production in the Huang-Huai-Hai region of China,resulting in huge yield losses(Xu et al.,2019).This disease has become an epidemic and prominent problem in soybean production and is still expanding its geography,including North America,posing a serious threat to soybean production(Harbach et al.,2016;Zhang et al.,2016;Li et al.,2019).However,the cause of soybean stay-green syndrome remains obscure. | Ruixiang Cheng Ruoxin Mei Rong Yan Hongyu Chen Dan Miao Lina Cai Jiayi Fan Gairu Li Ran Xu Weiguo Lu Yu Gao Wenwu Ye Shuo Su Tianfu Han Junyi Gai Yuanchao Wang Xiaorong Tao Yi Xu | 2022 | Molecular Plant2022,15,6: | 6 |
| 5 | NON-YELLOWING2 (NYE2), a Close Paralog of NYE1, Plays a Positive Role in Chlorophyll Degradation in Arabidopsis显示文摘 | Wu, Shouxin Li, Zhongpeng Yang, Lifeng Xie, Zuokun Chen, Junyi Zhang, Wei Liu, Tianqi Gao, Shan Gao, Jiong Zhu, Yihua Xin, Jiwen Ren, Guodong Kuai, Benke | 2016 | Molecular Plant2016,9,4: | 6 |
| 6 | Atmospheric isoprene and monoterpenes in a typical urban area of Beijing: Pollution characterization, chemical reactivity and source identification显示文摘Continuous observation of isoprene, α-pinene and β-pinene was carried out in a typical urban area of Beijing from March 2014 to February 2015, using an AirmoV OC online analyzer. Based on the analysis of the ambient level and variation characteristics of isoprene, α-pinene and β-pinene, the chemical reactivity was studied, and their sources were identified. Results showed that the concentrations of isoprene, α-pinene and β-pinene in the urban area of Beijing were lower than those in richly vegetated areas; the concentrations of isoprene were at a moderate level compared with those of previous studies of Beijing. Concentrations of isoprene, α-pinene and β-pinene showed different seasonal, monthly, daily and diurnal variations, and all of the three species showed higher level at night than those in the daytime as a whole, the variations of isoprene, α-pinene and β-pinene mainly influenced by emission of sources, photochemical reaction, and meteorological parameters. Isoprene was the largest contributor to the total OFP values than α-pinene and β-pinene. α-Pinene was the largest contributor to the total SOAFP values than isoprene and β-pinene in autumn, while isoprene was the largest one in other seasons. Isoprene, α-pinene and β-pinene were derived mainly from biological sources; and α-pinene level were also affected by industrial sources. To reduce the concentrations of isoprene, α-pinene and β-pinene, it is necessary to scientifically select urban green plant species, and more strict control measures should be taken to reduce the emission of α-pinene from industrial sources, such as artificial flavors and resins synthesis processes. | Xi Cheng Hong Li Yujie Zhang Yuping Li Weiqi Zhang Xuezhong Wang Fang Bi Hao Zhang Jian Gao Fahe Chai Xiaoxiu Lun Yizhen Chen Junyi Lv | 2018 | Journal of Environmental Sciences2018,30,9: | 3 |
| 7 | Effect of different film color mulching on dry matter and grain yield of oil flax in dry-land显示文摘Mulching can effectively maintain soil moisture;color of mulching film affects soil water storage capacity and further promote crop growth to improve grain yield.Field experiment was conducted to study effects of different film colors on dry matter accumulation(DMA)and grain yield of oil flax.Results showed that white plastic film mulching could increase leaf area,chlorophyll content and DMA.DMA of white film mulching and micro-ridge with soil covering was 53.0%higher than that of CK,and 7.8%higher than that of black film mulching.Mulching method also influenced DMA.Micro-ridge alone increased it by 13.7%than flat cultivation and soil covering improved another 7.6%under white film mulching.Both white and black film mulching with microridge could significantly improve grain yield.Black film mulching with micro-ridge and soil covering,white film mulching with micro-ridge and no soil covering significantly increased capsule number per plant,1,000-grain weight and grain yield of oil flax,compared with CK.Grain yield increased 29.0%and 28.9%respectively.These results indicated that the above mulching methods were suitable for high yield cropping pattern in dry-farming regions. | Yuhong Gao Ying Li Yingze Wang Bing Wu Jia Ke Junyi Niu Lizhuo Guo | 2020 | Oil Crop Science2020,5,2: | 2 |
| 8 | High-performance modified uni-traveling carrier photodiode integrated on a thin-film lithium niobate platform显示文摘Lithium niobate on insulator(LNOI)has become an intriguing platform for integrated photonics for applications in communications,microwave photonics,and computing.Whereas,integrated devices including modulators,resonators,and lasers with high performance have been recently realized on the LNOI platform,high-speed photodetectors,an essential building block in photonic integrated circuits,have not been demonstrated on LNOI yet.Here,we demonstrate for the first time,heterogeneously integrated modified uni-traveling carrier photodiodes on LNOI with a record-high bandwidth of 80 GHz and a responsivity of 0.6 A/W at a 1550-nm wavelength.The photodiodes are based on an n-down In GaAs/InP epitaxial layer structure that was optimized for high carrier transit time-limited bandwidth.Photodiode integration was achieved using a scalable wafer die bonding approach that is fully compatible with the LNOI platform. | XIANGWEN GUO LINBO SHAO LINGYAN HE KEVIN LUKE JESSE MORGAN KEYE SUN JUNYI GAO TA-CHING TZU YANG SHEN DEKANG CHEN BINGTIAN GUO FENGXIN YU QIANHUAN YU MASOUD JAFARI MARKO LONčAR MIAN ZHANG ANDREAS BELING | 2022 | Photonics Research2022,10,6: | 2 |
| 9 | 1-Pbps orbital angular momentum fibre-optic transmission显示文摘Space-division multiplexing(SDM),as a main candidate for future ultra-high capacity fibre-optic communications,needs to address limitations to its scalability imposed by computation-intensive multi-input multi-output(MIMO)digital signal processing(DSP)required to eliminate the crosstalk caused by optical coupling between multiplexed spatial channels.By exploiting the unique propagation characteristics of orbital angular momentum(OAM)modes in ring core fibres(RCFs),a system that combines SDM and C+L band dense wavelength-division multiplexing(DWDM)in a 34 km 7-core RCF is demonstrated to transport a total of 24960 channels with a raw(net)capacity of 1.223(1.02)Peta-bit s−1(Pbps)and a spectral efficiency of 156.8(130.7)bit s−1 Hz−1.Remarkably for such a high channel count,the system only uses fixed-size 4×4 MIMO DSP modules with no more than 25 time-domain taps.Such ultra-low MIMO complexity is enabled by the simultaneous weak coupling among fibre cores and amongst non-degenerate OAM mode groups within each core that have a fixed number of 4 modes.These results take the capacity of OAM-based fibre-optic communications links over the 1 Pbps milestone for the first time.They also simultaneously represent the lowest MIMO complexity and the 2nd smallest fibre cladding diameter amongst reported few-mode multicore-fibre(FM-MCF)SDM systems of>1 Pbps capacity.We believe these results represent a major step forward in SDM transmission,as they manifest the significant potentials for further up-scaling the capacity per optical fibre whilst keeping MIMO processing to an ultra-low complexity level and in a modularly expandable fashion. | Junyi Liu Jingxing Zhang Jie Liu Zhenrui Lin Zhenhua Li Zhongzheng Lin Junwei Zhang Cong Huang Shuqi Mo Lei Shen Shuqing Lin Yujie Chen Ran Gao Lei Zhang Xiaobo Lan Xinlun Cai Zhaohui Li Siyuan Yu | 2022 | Light(Science & Applications)2022,11,8: | 1 |
| 10 | Integrated transcriptome and metabolite profiling highlights the role of benzoxazinoids in wheat resistance against Fusarium crown rot显示文摘Fusarium crown rot(FCR), caused by Fusarium spp., is a chronic and severe plant disease worldwide. In the last years, the incidence and severity of FCR in China has increased to the point that it is now considered a threat to local wheat crops. In this study, for the first time, the metabolites and transcripts responsive to FCR infection in the partial resistant wheat cultivar 04 Zhong 36(04 z36) and susceptible cultivar Xinmai 26(XM) were investigated and compared at 20 and 25 days post inoculation(dpi). A total of 443 metabolites were detected, of which 102 were significantly changed because of pathogen colonization.Most of these 102 metabolites belonged to the flavonoid, phenolic acid, amino acid and derivative classes.Some metabolites, such as proline betaine, lauric acid, ribitol, and arabitol, were stably induced by Fusarium pseudograminearum(Fp) infection at two time points and may have important roles in FCR resistance. In line with the reduced seedling height of 04 z36 and XM plants, RNA-seq analysis revealed that FCR infection significantly affected the photosynthesis activities in two cultivars. Furthermore, 15 jasmonate ZIM-domain genes(JAZ) in the significantly enriched ‘regulation of jasmonic acid mediated signaling pathway’ in 04 z36 were down-regulated. The down-regulation of these JAZ genes in 04 z36 may cause a strong activation of the jasmonate signaling pathway. Based on combined data from gene expression and metabolite profiles, two metabolites, benzoxazolin-2-one(BOA) and 6-methoxy-benzoxazolin-2-one(MBOA), involved in the benzoxazinoid-biosynthesis pathway, were tested for their effects on FCR resistance. Both BOA and MBOA significantly reduced fungal growth in vitro and in vivo, and, thus, a higher content of BOA and MBOA in 04 z36 may contribute to FCR resistance. Above all, the current analysis extends our understanding of the molecular mechanisms of FCR resistance/susceptibility in wheat and will benefit further efforts for the genetic improvement of disease resistance. | Shuonan Duan Jingjing Jin Yutian Gao Changlin Jin Junyi Mu Wenchao Zhen Qixin Sun Chaojie Xie Jun Ma | 2022 | The Crop Journal2022,10,2: | 1 |
| 11 | The effect of temperature on gonad,embryonic development and survival rate of juvenile seahorses,Hippocampus kuda Bleeker显示文摘 | LIN Qiang LU Junyi GAO Yongli | 2006 | Aquaculture2006,254,: | 1 |
| 12 | Theory-driven designed TiO_(2)@MoO_(2)heterojunction:Balanced crystallinity and nanostructure toward desirable kinetics and highrate sodium-ion storage显示文摘Sodium-ion batteries(SIBs)are promising candidates for large-scale energy storage due to their cost effectiveness and the unlimited availability of sodium.However,there remains a need for the rational design of better anodic materials than are currently available,as these materials are critical for the sodium-ion storage process.In this work,theoretical calculations were performed to design a conceptually novel TiO_(2)@MoO_(2)heterojunction(TMH)anode that was expected to exhibit better electrochemical performance than current anodes.The TMH anode was fabricated via a facile and cost-effective method,and the results of in-depth sodium-ion-storage performance and reaction kinetics analyses indicate that it exhibited an excellent rate capability and enhanced pseudocapacitive response,due to its high crystallinity.This electrochemical performance was superior to that of previously reported anodic materials,confirming the accuracy of the theoretical calculations.Destruction of TMH’s nanostructure at high temperatures resulted in a decrease in its electrochemical performance,indicating the key role played by the nanostructure in TMH’s ability to store sodium ions.This study demonstrates that integration of theoretical predictions with experimental investigations offers insights into how materials’crystallinity and nanostructure affect their pseudocapacitive sodiumion storage capabilities,which will help to guide the rational design of effective anodic materials for SIBs. | Junyi Yin Pengqi Hai Yuan Gao Zihan Gan Chao Wu Yonghong Cheng Xin Xu | 2023 | Nano Research2023,16,4: | 1 |
| 13 | Estimation of chlorophyll content in maize canopy using wavelet denoising and SVR method显示文摘In order to estimate the chlorophyll content of maize plant non-destructively and rapidly,the research was conducted on maize at the heading stage using spectroscopy technology.The spectral reflectance of maize canopy was measured and processed following wavelet denoising and multivariate scatter correction(MSC)to reduce the noise influence.Firstly,the signal to noise ratio(SNR)and curve smoothness(CS)were used to evaluate the denoising effect of different wavelet functions and decomposition levels.As a result,the Sym6 wavelet basis function and the 5th level decomposition were determined to denoise the original signal.The MSC method was used to eliminate the scattering effect after denoising.Then three spectral ranges were extracted by interval partial least squares(IPLS)including the 525-549 nm,675-749 nm and 850-874 nm.Finally,the chlorophyll content estimation model was developed by using support vector regression(SVR)method.The calibration Rc2 of the SVR model was 0.831,the RMSEC was 1.3852 mg/L;the validation Rv2 was 0.809,the RMSEP was 0.8664 mg/L.The results show that the SNR and CS indicators can be used to select the parameters for wavelet denoising and model can be used to estimate the chlorophyll content of maize canopy in the field. | Haojie Liu Minzan Li Junyi Zhang Dehua Gao Hong Sun Liwei Yang | 2018 | International Journal of Agricultural and Biological Engineering2018,11,6: | 1 |
| 14 | Agronomic cultivation measures on productivity of oilseed flax:A review显示文摘Oilseed flax is one of the most important oil crops in China.With the improvement of people's living standards and the deepening knowledge of the nutritional value of oilseed flax,the demand and economic value of oilseed flax are increasing,and the cultivated area in China is expanding.However,the grain yield of oilseed flax is lower than other oil crops.It varies significantly from year to year,combined with a lower degree of mechanization,which has greatly limited the healthy development of the flax industry.Some of the effects of agronomic measures on productivity and water use efficiency of oilseed flax are reviewed in this paper.The major agronomic strategies for the productivity of oilseed flax were presented based on fertilization,plant density,irrigation,cropping pattern and weed control.Future research should investigate the effect of silicon and potassium fertilizers on the mechanism of lodging resistance of oilseed flax,the effects of diversified cropping systems(strip intercropping and crop rotation)on high and stable productivity and efficient utilization of resources. | Zhengjun Cui Bin Yan Yuhong Gao Bing Wu Yifan Wang Haidi Wang Peng Xu Bangqing Zhao Zhi Cao Yong Zhang Yaping Xie Yapeng Hu Xingbang Ma Junyi Niu | 2022 | Oil Crop Science2022,7,1: | 1 |
| 15 | Genetic parameters and response to selection for harvest body weight of the giant freshwater prawn Macrobrachium rosenbergii显示文摘 | Sheng Luan Guoliang Yang Junyi Wang Kun Luo Yufei Zhang Qiang Gao Honglang Hu Jie Kong | 2012 | Aquaculture2012,,: | 1 |
| 16 | Regional joint PM_(2.5)-O_(3) control policy benefits further air quality improvement and human health protection in Beijing-Tianjin-Hebei and its surrounding areas显示文摘Beijing-Tianjin-Hebei and its surrounding areas(hereinafter referred to as“2+26”cities)are one of the most severe air pollution areas in China.The fine particulate matter(PM_(2.5))and surface ozone(O_(3))pollution have aroused a significant concern on the national scale.In this study,we analyzed the pollution characteristics of PM_(2.5) and O_(3) in“2+26”cities,and then estimated the health burden and economic loss before and after the implementation of the joint PM_(2.5)-O_(3) control policy.During 2017–2019,PM_(2.5) concentration reduced by 19%while the maximum daily 8 hr average(MDA8)O_(3) stayed stable in“2+26”cities.Spatially,PM_(2.5) pollution in the south-central area and O_(3) pollution in the central region were more severe than anywhere else.With the reduction in PM_(2.5) concentration,premature deaths fromPM_(2.5) decreased by 18%from 2017 to 2019.In contrast,premature deaths from O_(3) increased by 5%.Noticeably,the huge potential health benefits can be gained after the implementation of a joint PM_(2.5)-O_(3) control policy.The premature deaths attributed to PM_(2.5) and O_(3) would be reduced by 91.6%and 89.1%,and the avoidable economic loss would be 60.8 billion Chinese Yuan(CNY),and 68.4 billion CNY in 2035 compared with that in 2019,respectively.Therefore,it is of significance to implement the jointPM_(2.5)-O_(3) control policy for improving public health and economic development. | Junyi Wang Aifang Gao Shaorong Li Yuehua Liu Weifeng Zhao Peng Wang Hongliang Zhang | 2023 | Journal of Environmental Sciences2023,,8: | 1 |
| 17 | Reducing the vulnerability of net- work by inserting modular topologies 显示文摘 | Zou Zhiyun Lai Junyi Gao Jianzhi | 2013 | Chaos: An Interdiscipli- nary Journal of Nonlinear Science2013,23,01: | 1 |
| 18 | A novel wavelength selection strategy for chlorophyll prediction by MWPLS and GA显示文摘The research proposed a novel wavelength selection strategy by the combination of moving window partial least squares(MWPLS)and genetic algorithm(GA)for the chlorophyll content detection of winter wheat canopy using spectroscopy technology.Firstly,the original spectral dataset was pre-processed by wavelet denosing,multiple scatter correction.Then,abnormal data samples were removed by Pauta Criterion and the dataset was divided into modeling set and validation set by SPXY.Finally,the sensitive wavebands were selected using MWPLS method and MWPLS+GA respectively and partial least squares(PLS)models were established for chlorophyll content prediction.For the model established by using all the wavebands in the region of 400-900 nm,its R_(c)^(2) and R_(v)^(2) were 0.4468 and 0.3821 respectively;its modeling root mean square error(RMSEM)and verification root mean square error(RMSEV)were 2.9057 and 1.7589 respectively.For the model established by using 151 wavebands selected by MWPLS,its R_(c)^(2) and R_(v)^(2) were 0.6210 and 0.5901 respectively;its RMSEM and RMSEV were 2.4007 and 1.6408 respectively.For the model established by using 36 wavebands selected by MWPLS+GA,its R_(c)^(2) and R_(v)^(2) were 0.7805 and 0.7497 respectively;its RMSEM and RMSEV were 1.8504 and 1.1315 respectively.The results show that wavelength selection can remove redundant information and improve model performance.The strategy of combining MWPLS with GA has also been proved to work well in selecting sensitive wavebands for chlorophyll content prediction. | Haojie Liu Minzan Li Junyi Zhang Dehua Gao Hong Sun Man Zhang Jingzhu Wu | 2019 | International Journal of Agricultural and Biological Engineering2019,12,5: | 1 |
| 19 | Long noncoding RNA(lncRNA)H19:An essential developmental regulator with expanding roles in cancer,stem cell differentiation,and metabolic diseases显示文摘Recent advances in deep sequencing technologies have revealed that,while less than 2%of the human genome is transcribed into mRNA for protein synthesis,over 80%of the genome is transcribed,leading to the production of large amounts of noncoding RNAs(ncRNAs).It has been shown that ncRNAs,especially long non-coding RNAs(lncRNAs),may play crucial regulatory roles in gene expression.As one of the first isolated and reported lncRNAs,H19 has gained much attention due to its essential roles in regulating many physiological and/or pathological processes including embryogenesis,development,tumorigenesis,osteogen-esis,and metabolism.Mechanistically,H19 mediates diverse regulatory functions by serving as competing endogenous RNAs(CeRNAs),Igf2/H19 imprinted tandem gene,modular scaffold,cooperating with H19 antisense,and acting directly with other mRNAs or lncRNAs.Here,we summarized the current understanding of H19 in embryogenesis and development,cancer development and progression,mesenchymal stem cell lineage-specific differentiation,and metabolic diseases.We discussed the potential regulatory mechanisms underlying H19’s func-tions in those processes although more in-depth studies are warranted to delineate the exact molecular,cellular,epigenetic,and genomic regulatory mechanisms underlying the physiolog-ical and pathological roles of H19.Ultimately,these lines of investigation may lead to the development of novel therapeutics for human diseases by exploiting H19 functions. | Junyi Liao Bowen Chen Zhenglin Zhu Chengcheng Du Shengqiang Gao Guozhi Zhao Piao Zhao Yonghui Wang Annie Wang Zander Schwartz Lily Song Jeffrey Hong William Wagstaff Rex CHaydon Hue HLuu Jiaming Fan Russell RReid Tong-Chuan He Lewis Shi Ning Hu Wei Huang | 2023 | Genes & Diseases2023,10,4: | 1 |
| 20 | A highly accessible copper single-atom catalyst for wound antibacterial application显示文摘Bacterial infection arised from multipathogenic bacteria is a tricky issue that attracts worldwide attentions.In this paper,a highly accessible copper single-atom catalyst(Cu SAC)supported by biocompatible N-doped mesoporous carbon nanospheres was synthesized with the emulsion-template method.The tightly anchored copper single-atom of the catalyst could effectively transform O_(2) into O_(2)−•under ambient conditions by the ultra-large pore size(~23.80 nm)and small particle size(~97.71 nm).Due to multiple synergistically oxidative damages to biomolecules,the Cu SAC could be employed to eliminate different bacteria in vitro without the generation of multidrug resistance(MDR).Moreover,the Cu SAC could also promote wound healing in vivo by eradicating the propagation of bacteria at wound.It is envisioned that the Cu SAC with superior antibacterial performance could be applied in the treatment of related bacterial infection in future. | Yue Zhao Yunpeng Yu Feng Gao Zhiyuan Wang Wenxing Chen Cai Chen Jia Yang Yancai Yao Junyi Du Chao Zhao Yuen Wu | 2021 | Nano Research2021,14,12: | 1 |