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| 1 | Developing a bacteriophage cocktail for biocontrol of potato bacterial wilt显示文摘Bacterial wilt is a devastating disease of potato and can cause an 80% production loss. To control wilt using bacteriophage therapy, we isolated and characterized twelve lytic bacteriophages from different water sources in Kenya and China. Based on the lytic curves of the phages with the pathogen Ralstonia solanacearum, one optimal bacteriophage cocktail, P1, containing six phage isolations was formulated and used for studying wilt prevention and treatment efficiency in potato plants growing in pots. The preliminary tests showed that the phage cocktail was very effective in preventing potato bacterial wilt by injection of the phages into the plants or decontamination of sterilized soil spiked with R. solanacearum. Eighty percent of potato plants could be protected from the bacterial wilt(caused by R. solanacearum reference strain GIM1.74 and field isolates), and the P1 cocktail could kill 98% of live bacteria spiked in the sterilized soil at one week after spraying.However, the treatment efficiencies of P1 depended on the timing of application of the phages, the susceptibility of the plants to the bacterial wilt, as well as the virulence of the bacteria infected,suggesting that it is important to apply the phage therapy as soon as possible once there are early signs of the bacterial wilt. These results provide the basis for the development of bacteriophagebased biocontrol of potato bacterial wilt as an alternative to the use of antibiotics. | Cuihua Wei Junli Liu Alice Nyarnbura Maina Francis B Mwaura Junping Yu Chenghui Yan Ruofang Zhang Hongping Wei | 2017 | Virologica Sinica2017，32，6： | 8 |
| 2 | Bacteriophages Isolated in China for the Control of Pectobacterium carotovorum Causing Potato Soft Rot in Kenya显示文摘Soft rot is an economically significant disease in potato and one of the major threats to sustainable potato production.This study aimed at isolating lytic bacteriophages and evaluating methods for and the efficacy of applying phages to control potato soft rot caused by Pectobacterium carotovorum.Eleven bacteriophages isolated from soil and water samples collected in Wuhan,China,were used to infect P.carotovorum host strains isolated from potato tubers showing soft rot symptoms in Nakuru county,Kenya.The efficacy of the phages in controlling soft rot disease was evaluated by applying individual phage strains or a phage cocktail on potato slices and tubers at different time points before or after inoculation with a P.carotovorum strain.The phages could lyse 20 strains of P.carotovorum,but not Pseudomonas fluorescens control strains.Among the 11 phages,Pectobacterium phage Wc5r,interestingly showed cross-activity against Pectobacterium atrosepticum and two phage-resistant P.carotovorum strains.Potato slice assays showed that the phage concentration and timing of application are crucial factors for effective soft rot control.Phage cocktail applied at a concentration of 1×10^9 plaque-forming units per milliliter before or within an hour after bacterial inoculation on potato slices,resulted in>90%reduction of soft rot symptoms.This study provides a basis for the development and application of phages to reduce the impact of potato soft rot disease. | Peter Muturi Junping Yu Alice Nyambura Maina Samuel Kariuki Frantis B.Mwaura Hongping Wei | 2019 | Virologica Sinica2019，34，3： | 4 |
| 3 | Clinical impact and mechanisms of hepatitis B virus infection concurrent with non-alcoholic fatty liver disease显示文摘Chronic hepatitis B(CHB)virus infection is an important threat to global health despite the administration of vaccines and the use of antiviral treatments.In recent years,as the prevalence of obesity and metabolic syndrome has increased,non-alcoholic fatty liver disease(NAFLD)in patients with CHB has become more common.Both diseases can lead to liver fibrosis and even hepatocellular carcinoma,but the risk of dual etiology,outcome,and CHB combined with NAFLD is not fully elucidated.In this review,we assess the overlapping prevalence of NAFLD and CHB,summarize recent studies of clinical and basic research related to potential interactions,and evaluate the progressive changes of treatments for CHB patients with NAFLD.This review increases the understanding of the relationship and mechanisms of interaction between steatosis and hepatitis B virus infection,and it provides new strategies for the future clinical management and treatment of CHB combined with NAFLD. | Xin Tong Yu Song Shengxia Yin Jian Wang Rui Huang Chao Wu Junping Shi Jie Li | 2022 | Chinese Medical Journal2022，，14： | 4 |
| 4 | Gender associates with both susceptibility to infection and pathogenesis of SARS-CoV-2 in Syrian hamster显示文摘Epidemiological studies of the COVID-19 patients have suggested the male bias in outcomes of lung illness.To experimentally demonstrate the epidemiological results,we performed animal studies to infect male and female Syrian hamsters with SARS-CoV-2.Remarkably,high viral titer in nasal washings was detectable in male hamsters who presented symptoms of weight loss,weakness,piloerection,hunched back and abdominal respiration,as well as severe pneumonia,pulmonary edema,consolidation,and fibrosis.In contrast with the males,the female hamsters showed much lower shedding viral titers,moderate symptoms,and relatively mild lung pathogenesis.The obvious differences in the susceptibility to SARS-CoV-2 and severity of lung pathogenesis between male and female hamsters provided experimental evidence that SARS-CoV-2 infection and the severity of COVID-19 are associated with gender. | Lunzhi Yuan Huachen Zhu Ming Zhou Jian Ma Rirong Chen Yao Chen Liqiang Chen Kun Wu Minping Cai Junping Hong Lifeng Li Che Liu Huan Yu Yali Zhang Jia Wang Tianying Zhang Shengxiang Ge Jun Zhang Quan Yuan Yixin Chen Qiyi Tang Honglin Chen Tong Cheng Yi Guan Ningshao Xia | 2021 | Signal Transduction and Targeted Therapy2021，6，4： | 3 |
| 5 | Enriched Opportunistic Pathogens Revealed by Metagenomic Sequencing Hint Potential Linkages between Pharyngeal Microbiota and COVID-19显示文摘As a respiratory tract virus,SARS-CoV-2 infected people through contacting with the upper respiratory tract first.Previous studies indicated that microbiota could modulate immune response against pathogen infection.In the present study,we performed metagenomic sequencing of pharyngeal swabs from eleven patients with COVID-19 and eleven Non-COVID-19 patients who had similar symptoms such as fever and cough.Through metagenomic analysis of the above two groups and a healthy group from the public data,there are 6502 species identified in the samples.Specifically,the Pielou index indicated a lower evenness of the microbiota in the COVID-19 group than that in the Non-COVID-19 group.Combined with the linear discriminant analysis(LDA)and the generalized linear model,eighty-one bacterial species were found with increased abundance in the COVID-19 group,where 51 species were enriched more than 8 folds.The top three enriched genera were Streptococcus,Prevotella and Campylobacter containing some opportunistic pathogens.More interestingly,through experiments,we found that two Streptococcus strains,S.suis and S.agalactiae,could stimulate the expression of ACE2 of Vero cells in vitro,which may promote SARS-CoV-2 infection.Therefore,these enriched pathogens in the pharynxes of COVID-19 patients may involve in the virus-host interactions to affect SARS-CoV-2 infection and cause potential secondary bacterial infections through changing the expression of the viral receptor ACE2 and/or modulate the host’s immune system. | Dongyan Xiong Caroline Muema Xiaoxu Zhang Xinming Pan Jin Xiong Hang Yang Junping Yu Hongping Wei | 2021 | Virologica Sinica2021，36，5： | 3 |
| 6 | Author correction to'Ruscogenin alleviates LPS-triggered pulmonary endothelial barrier dysfunction through targeting NMMHC IIA to modulate TL R4 signaling’[Acta Pharmaceutica Sinica B 12(2022)1198-1212]显示文摘The authors regret that there were some errors or inexact de-scriptions in author afiliation,the section of materials and methods,and the figure caption of Fig.2A owing to the negligence of authors when writing the manuscript,and there was a picture error in Fig.10 and graphical abstract figure owing to the inappropriate selection for the picture of Radix Ophiopogon japonicus.In author afiliation,'Department of Pharmacology of Chinese Material Medica'should be corrected to'Department of Pharmacology of Chinese Materia Medica'.In 2.4. | Yunhao Wu Xiu Yu Yuwei Wang Yalin Huang Jiahui Tang Shuaishuai Gong Siyu Jiang Yuanli Xia Fang Li Boyang Yu Yuanyuan Zhang Junping Kou | 2022 | Acta Pharmaceutica Sinica B2022，12，7： | 3 |
| 7 | Synthesis and characterization of super paramagnetic composite photocatalyst-Titania/silica/nickel ferrite显示文摘Composite photocatalysts with TiO2 porous shell and magnetic core were synthesized by using NiFe2O4 as a magnetic core, SiO2 as an intermediate layer and polyethylene glycol with molecular weight of 1000 (PEG 1000) as a template direction agent. The samples were characterized with N 2 adsorption-desorption, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), vibrating sample magnetometry (VSM) and ultraviolet-visible (UV-vis) spectroscopy. The results showed that the obtained composites are spherical particles about 50 nm in diameter. The mesoporous TiO 2 with magnetic core calcined at 350 C, 450 C, and 550 C was found to have, respectively, different specific surface areas (94, 86, and 68 m2 /g) and average pore sizes (1.4, 2.2, and 2.8 nm). The photocatalytic activity was investigated for degradation of nitrobenzene under UV irradiation. Nitrobenzene was decomposed in 300 min under irradiation of an 8 W UV lamp with a peak wavelength of 253.7 nm. The composite nanopar- ticles exhibited high photocatalytic efficiency and super paramagnetic nature for cleaning polluted water by magnetic separation. | Yu Li Xiufang Dong Junping Li | 2011 | Particuology2011，9，5： | 2 |
| 8 | Targeted thrombolysis strategies for neuroprotective effect显示文摘Stroke is usually treated by systemic thrombolytic therapy if the patient presents within an appropriate time window. There is also widespread interest in the development of thrombolytic agents that can be used in cases of delayed presentation. Current agents that can be used in cases of delayed presentation of nerve damage by thrombus. Current systemic thrombolytic therapy is associated with adverse effects such as fibrinogenolysis and bleeding. In an attempt to increase the efficacy, safety, and specificity of thrombolytic therapy, a number of targeted thrombolytic agents have been studied in recent years. This review focuses on the concepts underlying targeted thrombolytic therapy and describes recent drug developments in this field. | Junping Zhang Guoxing Ma Zhimin Lv Yu Zhou Chunguang Wen Yaqing Wu Ruian Xu | 2014 | Neural Regeneration Research2014，9，13： | 2 |
| 9 | Analysis of Focusing Orbital Angular Momentum Wave Using Fabry-Perot Cavity显示文摘One of the biggest obstacles to the application of orbital angular momentum(OAM)in the microwave field is its divergence problem.This paper presents a full analysis of generating and focusing OAM waves using original and improved Fabry-Perot(F-P)cavities.Utilizing combination of microstrip antenna and original F-P cavity,the gain of the OAM antenna is enhanced from 4.0 dBi to 9.3 dBi and the corresponding divergence angle is decreased from 41to 24.To further improve the performance of the OAM antenna,the improved F-P cavity is introduced.The simulated results show that the gain is further enhanced to 12.0 dBi and the divergence angle is further decreased to be 15. | Yu Yao Xianling Liang Ronghong Jin Junping Geng | 2019 | Journal of Communications and Information Networks2019，4，3： | 1 |
| 10 | An efficient CRISPR/Cas9 platform for targeted genome editing in rose(Rosa hybrida)显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-related nuclease 9(Cas9)system enables precise,simple editing of genes in many animals and plants.However,this system has not been applied to rose(Rosa hybrida)due to the genomic complexity and lack of an efficient transformation technology for this plant.Here,we established a platform for screening single-guide RNAs(sgRNAs)with high editing efficiency for CRISPR/Cas9-mediated gene editing in rose using suspension cells.We used the Arabidopsis thaliana U6-29 promoter,which showed high activity for driving sgRNA expression,to modify the CRISPR/Cas9 system.We used our highly efficient optimized CRISPR/Cas9 system to successfully edit RhEIN2,encoding an indispensable component of the ethylene signaling pathway,resulting in ethylene insensitivity in rose.Our optimized CRISPR/Cas9 system provides a powerful toolbox for functional genomics,molecular breeding,and synthetic biology in rose. | Chengpeng Wang Yang Li Na Wang Qin Yu Yonghong Li Junping Gao Xiaofeng Zhou Nan Ma | 2023 | Journal of Integrative Plant Biology2023，65，4： | 1 |
| 11 | Progress Toward Prevention of Transfusion-Transmitted Hepatitis B and Hepatitis C Infection - Sub-Saharan Africa, 2000-2011显示文摘 | Apata Ibironke W Averhoff Francisco Pitman John Bjork Adam Yu Junping Amin Noryati Abu Dhingra Neelam Kolwaite Amy Marfin Anthony | 2014 | Morbidity and Mortality Weekly Report2014，，29： | 1 |
| 12 | An AFM Observation on Fossil Cytoplasm显示文摘摘要:石块细胞质是对古植物学的兴趣的一个新研究话题。原子力量显微镜(AFM ) 是在物理和生物学广泛地使用的一种新技术;然而,它很少在古生物学被使用。这里,我们第一次使用了 AFM 学习石块细胞质。结果显示石块细胞质超微结构异构、完整,并且特别在有另外的技术的联合, AFM 的申请能揭示 subcellular,这与更多的信心石块植物详细说明,就像现存细胞质一样。 | WANG Xin YU Junping FANG Xiaohong | 2008 | Acta Geologica Sinica(English Edition)2008，82，6： | 1 |
| 13 | Detection and parameter estimation of LFM signal using integration of fractional Gaussian window transform显示文摘 | LI Jiaqiang JIN Ronghong GENG Junping FAN Yu MAO Wei | 2007 | IEICE Transactions on Communications2007，90，3： | 1 |
| 14 | Type2d Iabetesandsyndromecharacter Ist Icsof显示文摘 | Wei Junp Ing Zhou LIbo LIu Fang Zhong Zhen LIu Ya jun Cao A Im ei Wang XIu zhi Zhu Yan Yu Bo LIu XIa Zhang Yanni To ng XIaol In | | 0，，12： | 1 |
| 15 | First-principles investigation of the impurity-kink interaction in bcc iron显示文摘Using the first-principles self-consistent discrete variational method based on density functional theory, we investigated the effect of light impurities C and N on the electronic structure of kink on the [100](010) edge dislocation (ED) in bcc iron. Our energetic calculations show that the light impurities have a strong segregation tendency to enter the kink. The results of the charge distribution and the local den-sity of states indicate that the strong bonds between the impurity atoms and the neighboring Fe atoms are formed due to the hybridizations of impurity atoms 2p states and Fe 3d4s4p states. The introduction of light impurities can stabilize the kink system, and impedes the sideward motion of the kink in the [100](010) ED. This is, the light impurities induce a strong pinning effect on the [100](010) ED and may result in the solid solute hardening. | YU Tao CHEN LiQun WANG ChongYu QIU ZhengChen DU JunPing | 2008 | Chinese Science Bulletin2008，53，12： | 1 |
| 16 | Antinociceptive and anti-inflammatory activities of Aquilaria sinensis (Lour.) Gilg. Leaves extract显示文摘 | Minhua Zhou Honggang Wang Suolangjiba Junping Kou Boyang Yu | 2008 | Journal of Ethnopharmacology2008，，2： | 1 |
| 17 | Improving BDS broadcast ephemeris accuracy using ground-satellite-link observations显示文摘The BeiDou Navigation Satellite System(BDS)employs a hybrid constellation including GEO(Geosynchronous Earth Orbit),IGSO(Inclined Geosynchronous Orbit),and MEO(Medium Earth Orbit)satellites,where the GEO and IGSO satellites are critical to providing continuous and reliable Positioning,Navigation,and Timing(PNT)services in the Asia–Pacifc region.To handle the inconsistency between the satellite orbits and clocks in the broadcast ephemeris,which are determined by the Orbit Determination and Time Synchronization(ODTS)and the Two-way Satellite Time Frequency Transfer(TWSTFT)technique,respectively,we present the strategies using ground-satellite-link observations to improve the accuracy of broadcast ephemeris.The clock diferences between the ODTS and TWSTFT techniques are used for correcting the radial orbit component to derive the refned orbits,which are used to generate the refned broadcast ephemeris.The test results show the precision of the refned orbits is improved by 50–60%in the 3-h to 12-h predicted arcs for the GEO satellites,and by 40–50%for the IGSO satellites.Moreover,the validation using satellite laser ranging observations shows the mean precision of the refned broadcast ephemeris is improved by 27%compared to the original one.Applying the proposed strategies in the BDS Operational Control Segment(OCS),the time evolution of BDS Single Point Positioning(SPP)in the period from Jan.2016 to April 2021 is evaluated.The SPP accuracy is improved from 1.94,2.06 and 3.29 m to 1.39,1.85,and 2.39 m in the north,east,and up components,respectively.Further update with the inclusion of BDS-3 satellites improve the corresponding SPP precision to 0.68,0.70 and 1.91 m. | Junping Chen Jungang Wang Chao Yu Yize Zhang Bin Wang | 2022 | Satellite Navigation2022，3，1： | 1 |
| 18 | Isolation and complete genome sequence of a novel virulent mycobacteriophage, CASbig显示文摘Dear Editor,Bacteriophages are powerful tools for investigating and manipulating their hosts(Fernandes et al.,2014).This holds particularly true for mycobacteriophages,which have facilitated the development of mycobacterial genetic systems and have generated tools for the | Tieshan Teng Junping Yu Hang Yang Hongping Wei | 2015 | Virologica Sinica2015，30，1： | 1 |
| 19 | Involvement of rose aquaporin RhPIP1;1 in ethylene-regulated petal expansion through interaction with RhPIP2;1显示文摘 | Wen Chen Xia Yin Lei Wang Ji Tian Ruoyun Yang Daofeng Liu Zhenhao Yu Nan Ma Junping Gao | 2013 | Plant Molecular Biology2013，，3： | 1 |
| 20 | Inhibitory effects of ethanol extract from Radix Ophiopogon japonicus on venous thrombosis linked with its endothelium-protective and anti-adhesive activities显示文摘 | KOU Junping YU Boyang XU Qiang | | 0，，03： | 1 |
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