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| 1 | Rapid Detection of Filoviruses by Real-time TaqMan Polymerase Chain Reaction Assays显示文摘Ebola virus (EBOV) and Marburg virus (MARV) are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provide differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. In this study, the TaqMan RT-PCR assays targeting the nucleoprotein genes of the Zaire Ebolavirus (ZEBOV) and MARV were developed and their sensitivities and specificities were investigated. Our results indicated that the assays were able to make reliable diagnosis over a wide range of virus copies from 103 to 109, corresponding to the threshold of a standard RNA transcript. The results showed that there were about 1010 RNA copies per milliliter of virus culture supernatant, equivalent to 10,000 RNA molecules per infectious virion, suggesting the presence of many non-infectious particles. These data indicated that the TaqMan RT-PCR assays developed in this study will be suitable for future surveillance and specific diagnosis of ZEBOV and MARV in China. | Yi Huang Hongping Wei Yunpeng Wang Zhengli Shi Herve Raoul Zhiming Yuan | 2012 | Virologica Sinica2012，27，5： | 10 |
| 2 | Developing a bacteriophage cocktail for biocontrol of potato bacterial wilt显示文摘Bacterial wilt is a devastating disease of potato and can cause an 80% production loss. To control wilt using bacteriophage therapy, we isolated and characterized twelve lytic bacteriophages from different water sources in Kenya and China. Based on the lytic curves of the phages with the pathogen Ralstonia solanacearum, one optimal bacteriophage cocktail, P1, containing six phage isolations was formulated and used for studying wilt prevention and treatment efficiency in potato plants growing in pots. The preliminary tests showed that the phage cocktail was very effective in preventing potato bacterial wilt by injection of the phages into the plants or decontamination of sterilized soil spiked with R. solanacearum. Eighty percent of potato plants could be protected from the bacterial wilt(caused by R. solanacearum reference strain GIM1.74 and field isolates), and the P1 cocktail could kill 98% of live bacteria spiked in the sterilized soil at one week after spraying.However, the treatment efficiencies of P1 depended on the timing of application of the phages, the susceptibility of the plants to the bacterial wilt, as well as the virulence of the bacteria infected,suggesting that it is important to apply the phage therapy as soon as possible once there are early signs of the bacterial wilt. These results provide the basis for the development of bacteriophagebased biocontrol of potato bacterial wilt as an alternative to the use of antibiotics. | Cuihua Wei Junli Liu Alice Nyarnbura Maina Francis B Mwaura Junping Yu Chenghui Yan Ruofang Zhang Hongping Wei | 2017 | Virologica Sinica2017，32，6： | 8 |
| 3 | Bacteriophages, revitalized after 100 years in the shadow of antibiotics显示文摘The year 2015 marks 100 years since Dr.Frederick Twort discovered the'filterable lytic factor',which was later independently discovered and named 'bacteriophage' by Dr.Felix d’Herelle.On this memorable centennial,it is exciting to see a special issue published by Virologica Sinica on Phages and Therapy.In this issue,readers will not only fi nd that bacteriophage research is | Hongping Wei | 2015 | Virologica Sinica2015，30，1： | 7 |
| 4 | Preparation and characterization of 3-aminopropyl-triethoxysilane grafted montmorillonite and acid-activated montmorillonite显示文摘3-aminopropyltriethoxysilane grafted montmorillonites were synthesized with montmorillonite, acidactivated montmorillonites and 3-aminopropyltriethoxysilane in ethanol-water mixture. The resulting products were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TG). XRD patterns demonstrated that silane was intercalated into the montmorillonite gallery, indicated by increasing basal spacings. These intercalated silanes probably adopt bilayer arrangement models within the montmorillonite and acid-activated montmorillonites interlayer spaces. TG and DTG curves indicate that acidification results in a decrease of the thermal stability of the resultant montmorillonites. Silane grafting leads to a surface property transformation of montmorillonite from hydrophilicity to lipophilicity and an increase of the thermal stability of the condensed silanes. | SHEN Wei HE HongPing ZHU JianXi YUAN Peng MA YueHong LIANG XiaoLiang | 2009 | Chinese Science Bulletin2009，54，2： | 6 |
| 5 | Bacteriophages Isolated in China for the Control of Pectobacterium carotovorum Causing Potato Soft Rot in Kenya显示文摘Soft rot is an economically significant disease in potato and one of the major threats to sustainable potato production.This study aimed at isolating lytic bacteriophages and evaluating methods for and the efficacy of applying phages to control potato soft rot caused by Pectobacterium carotovorum.Eleven bacteriophages isolated from soil and water samples collected in Wuhan,China,were used to infect P.carotovorum host strains isolated from potato tubers showing soft rot symptoms in Nakuru county,Kenya.The efficacy of the phages in controlling soft rot disease was evaluated by applying individual phage strains or a phage cocktail on potato slices and tubers at different time points before or after inoculation with a P.carotovorum strain.The phages could lyse 20 strains of P.carotovorum,but not Pseudomonas fluorescens control strains.Among the 11 phages,Pectobacterium phage Wc5r,interestingly showed cross-activity against Pectobacterium atrosepticum and two phage-resistant P.carotovorum strains.Potato slice assays showed that the phage concentration and timing of application are crucial factors for effective soft rot control.Phage cocktail applied at a concentration of 1×10^9 plaque-forming units per milliliter before or within an hour after bacterial inoculation on potato slices,resulted in>90%reduction of soft rot symptoms.This study provides a basis for the development and application of phages to reduce the impact of potato soft rot disease. | Peter Muturi Junping Yu Alice Nyambura Maina Samuel Kariuki Frantis B.Mwaura Hongping Wei | 2019 | Virologica Sinica2019，34，3： | 4 |
| 6 | Dual-basis reconstruction techniques for tomographic PIV显示文摘As an inverse problem, particle reconstruction in tomographic particle image velocimetry attempts to solve a large-scale underdetermined linear system using an optimization technique. The most popular approach, the multiplicative algebraic reconstruction technique(MART), uses entropy as an objective function in the optimization. All available MART-based methods are focused on improving the efficiency and accuracy of particle reconstruction. However, those methods do not perform very well on dealing with ghost particles in highly seeded measurements. In this report, a new technique called dual-basis pursuit(DBP), which is based on the basis pursuit technique, is proposed for tomographic particle reconstruction. A template basis is introduced as a priori knowledge of a particle intensity distribution combined with a correcting basis to enable a full span of the solution space of the underdetermined linear system. A numerical assessment test with 2D synthetic images indicated that the DBP technique is superior to MART method, can completely recover a particle field when the number of particles per pixel(ppp) is less than 0.15, and can maintain a quality factor Q of above 0.8 for ppp up to 0.30. Unfortunately, the DBP method is difficult to utilize in 3D applications due to the cost of its excessive memory usage. Therefore, a dual-basis MART was designed that performed better than the traditional MART and can potentially be utilized for 3D applications. | YE ZhiJian GAO Qi WANG HongPing WEI RunJie WANG JinJun | 2015 | Science China(Technological Sciences)2015，58，11： | 4 |
| 7 | Source of propagules of the fouling green macroalgae in the Subei Shoal,China显示文摘Since 2007,large-scale green tides dominated by Ulva prolifera consecutively bloomed in the Yellow Sea and caused great economic losses.The fouling U.prolifera on the Pyropia yezoensis aquaculture rafts in the Subei Shoal was regarded as the major source of the floating biomass.However,it was still unclear about the seed source of fouling green macroalgae attached on the rafts.In this study,the field surveys and the indoor experiments were conducted to reveal the source of propagules of the fouling green macroalgae on the rafts and to study the anti-fouling material for P.yezoensis aquaculture rafts which could possibly be a feasible strategy to control the green tides in the Yellow Sea.The results showed that(1) micro-propagules of several green macroalgal species,including U.prolifera,U.linza,U.compressa,U.flexuosa,and Blidingia sp.coexisted in the waters and sediments in the Subei Shoal and their proportion remarkably changed over time;(2) the bamboo poles with peeling treatment could significantly reduce the amount of U.prolifera micro-propagules attached.This study confirmed that the micro-propagules distributed in the Subei Shoal area were the precursors of the green tides,and provided a feasible method to control the Yellow Sea large-scale green tides at the beginning. | SONG Wei JIANG Meijie WANG Zongling WANG Hongping ZHANG Xuelei FU Mingzhu | 2018 | Acta Oceanologica Sinica2018，37，4： | 3 |
| 8 | Amorphous TiO_2-supported Keggin-type ionic liquid catalyst catalytic oxidation of dibenzothiophene in diesel显示文摘Supported ionic liquid(IL) catalysts [C_nmim]_3PMo_(12)O_(40)/Am TiO_2(amorphous TiO_2) were synthesized through a one-step method for extraction coupled catalytic oxidative desulfurization(ECODS) system. Characterizations such as FTIR, DRS,wide-angle XRD, N_2 adsorption–desorption and XPS were applied to analyze the morphology and Keggin structure of the catalysts. In ECODS with hydrogen peroxide as the oxidant, it was found that ILs with longer alkyl chains in the cationic moiety had a better effect on the removal of dibenzothiophene. The desulfurization could reach 100% under optimal conditions, and GC–MS analysis was employed to detect the oxidized product after the reaction. Factors affecting the desulfurization efficiencies were discussed, and a possible mechanism was proposed. In addition, cyclic experiments were also conducted to investigate the recyclability of the supported catalyst. The catalytic activity of [C_(16)mim]_3 PMo_(12)O_(40)/Am TiO_2 only dropped from 100% to 92.9% after ten cycles, demonstrating the good recycling performance of the catalyst and its potential industrial application. | Zhendong Yu Donghui Wang Suhang Xun Minqiang He Ruliang Ma Wei Jiang Hongping Li Wenshuai Zhu Huaming Li | 2018 | Petroleum Science2018，15，4： | 3 |
| 9 | Enriched Opportunistic Pathogens Revealed by Metagenomic Sequencing Hint Potential Linkages between Pharyngeal Microbiota and COVID-19显示文摘As a respiratory tract virus,SARS-CoV-2 infected people through contacting with the upper respiratory tract first.Previous studies indicated that microbiota could modulate immune response against pathogen infection.In the present study,we performed metagenomic sequencing of pharyngeal swabs from eleven patients with COVID-19 and eleven Non-COVID-19 patients who had similar symptoms such as fever and cough.Through metagenomic analysis of the above two groups and a healthy group from the public data,there are 6502 species identified in the samples.Specifically,the Pielou index indicated a lower evenness of the microbiota in the COVID-19 group than that in the Non-COVID-19 group.Combined with the linear discriminant analysis(LDA)and the generalized linear model,eighty-one bacterial species were found with increased abundance in the COVID-19 group,where 51 species were enriched more than 8 folds.The top three enriched genera were Streptococcus,Prevotella and Campylobacter containing some opportunistic pathogens.More interestingly,through experiments,we found that two Streptococcus strains,S.suis and S.agalactiae,could stimulate the expression of ACE2 of Vero cells in vitro,which may promote SARS-CoV-2 infection.Therefore,these enriched pathogens in the pharynxes of COVID-19 patients may involve in the virus-host interactions to affect SARS-CoV-2 infection and cause potential secondary bacterial infections through changing the expression of the viral receptor ACE2 and/or modulate the host’s immune system. | Dongyan Xiong Caroline Muema Xiaoxu Zhang Xinming Pan Jin Xiong Hang Yang Junping Yu Hongping Wei | 2021 | Virologica Sinica2021，36，5： | 3 |
| 10 | A comparative study of the extractive desulfurization mechanism by Cu(Ⅱ)and Zn-based imidazolium ionic liquids显示文摘A comparative study of the extractive desulfurization(EDS) mechanism by Cu(Ⅱ) and Zn-based ILs([C_4mim]_2[MCl_4], M ? Cu(Ⅱ) or Zn)has been performed. It is found that the p–p interaction and C-H/p interaction play important roles in EDS for both Cu(Ⅱ) and Zn-based ILs,which is different from Al, Fe-based ILs. In the gas phase models, the interaction energy between Zn-based ILs and dibenzothiophene(DBT) is stronger than the interaction energy of Cu(Ⅱ)-based ILs. In order to consider the solvent effect, a generic ionic liquid of solvation model has been implemented, which is few considered in the previous calculations of EDS. It is interesting to find that the gap of interaction energies between Cu(Ⅱ), Zn-based ILs and DBT are reduced when the solvent effect is considered. In addition, by combined discussion of currently theoretical and experimental evidences for metal-based ILs with different compositions, we firstly propose that the EDS performance should be influenced by the balance of the contribution of cation, metal-based anion, metal chlorides and the viscosity. | Hongping Li Beibei Zhang Wei Jiang Wenshuai Zhu Ming Zhang Chao Wang Jingyu Pang Huaming Li | 2019 | Green Energy & Environment2019，4，1： | 2 |
| 11 | Surgical treatment of hepato-pancreato-biliary disease in China:the Tongji experience显示文摘Hepato-pancreato-biliary(HPB) tumors are common in China. However, these tumors are often diagnosed at intermediate/advanced stages because of the lack of a systemic surveillance program in China. This situation creates many technical challenges for surgeons and increases the incidence of postoperative complications. Therefore, Dr. Xiao-Ping Chen has made many important technical improvements, such as Chen's hepatic portal occlusion method, the anterior approach for liver resection of large HCC tumors, the modified technique of Belghiti's liver-hanging maneuver, inserting biliary-enteric anastomosis technique, and invaginated pancreaticojujunostomy with transpancreatic U-sutures. These techniques are simple, practical, and easy to learn. Owing to these advantages, complicated surgical procedures can be simplified, and the curative effects are greatly improved. These improved techniques have been widely applied in China and will benefit many additional patients. In this review, we introduce our experience of surgically treating intermediate/advanced hepatocellular carcinoma(HCC), hilar cholangiocarcinoma(HC), and pancreatic carcinoma, mainly focusing on technical innovations established by Dr. Chen in HPB surgery. | binhao zhang wei dong hongping luo xuanru zhu lin chen changhai li wei zhang shuai xiang wanguang zhang zhiyong huang xiao-ping chen | 2016 | Science China(Life Sciences)2016，59，10： | 2 |
| 12 | Extensive evolution analysis of the global chikungunya virus strains revealed the origination of CHIKV epidemics in Pakistan in 2016显示文摘Chikungunya virus(CHIKV) is a mosquito-borne virus that causes epidemics widely in the world especially in the tropical and subtropical regions. Phylogenetic analysis has found that the CHIKV lineages were associated with the spatial and temporal distributions, which were related to the virus adaption to the major mosquito species and their distributions. In this study, we reported the complete genome sequences of eight CHIKV isolates from the outbreak in Pakistan last year. Then we reviewed the evolutionary history using extensive phylogenetic analysis, analyzed lineagespecific substitutions in viral proteins, and characterized the spreading pathway of CHIKV strains including the Pakistani strains. The results showed that the Pakistani stains belonged to the ECSA.IOL sub-lineage and derived from India. The genetic properties of the Pakistani strains including the adaptive substitution to vectors were further characterized, and the potential risks from the occurrence of CHIKV infection in Pakistan were discussed. These results provided better understanding of CHIKV evolution and transmission in the world and revealed the possible origination of the CHIKV outbreak and epidemic in Pakistan, which would promote the disease prevention and control in the identified countries and territories with the history of CHIKV infections as well as new regions with potential risk of CHIKV outbreaks. | Junming Shi Zhengyuan Su Zhaojun Fan Jun Wang Siqing Liu Bo Zhang Hongping Wei Shoukat Jehan Nadia Jamil Shu Shen Fei Deng | 2017 | Virologica Sinica2017，32，6： | 2 |
| 13 | Gold nanoparticle enhanced immuno-PCR for ultrasensitive detection of Hantaan virus nucleocapsid protein显示文摘 | Longyan Chen Hongping Wei Yongchao Guo Zongqiang Cui Zhiping Zhang Xian-En Zhang | 2009 | Journal of Immunological Methods2009，，： | 1 |
| 14 | Grafting of montmorillonite with different functional silanes via two different reactionsystems显示文摘 | Shen Wei He Hongping Zhu Jianxi | 2007 | Journal of Colloid and Interface Science2007，313，1： | 1 |
| 15 | Heritable alteration in DNA methylation pattern occurred specifically at mobile elements in rice plants following hydrostatic pressurization显示文摘 | Likun Long Xiuyun Lin Jinzuo Zhai Hongping Kou Wei Yang Bao Liu | 2005 | Biochemical and Biophysical Research Communications2005，，2： | 1 |
| 16 | Isolation and complete genome sequence of a novel virulent mycobacteriophage, CASbig显示文摘Dear Editor,Bacteriophages are powerful tools for investigating and manipulating their hosts(Fernandes et al.,2014).This holds particularly true for mycobacteriophages,which have facilitated the development of mycobacterial genetic systems and have generated tools for the | Tieshan Teng Junping Yu Hang Yang Hongping Wei | 2015 | Virologica Sinica2015，30，1： | 1 |
| 17 | Gold nanoparticle enhanced immuno-PCR for ultrasensitive detection of Haataan virus nucleocapsid protein 显示文摘 | Chen Longyan Wei Hongping Guo Yongchao | 2009 | J Immunol Methods2009，346，12： | 1 |
| 18 | A recognition system of China-style license plates based on mathematical morphology and neural network显示文摘 | Yanping Bai Hongping Hu Fei Li Wei Shuang Lixia Gong | 2010 | International Journal of Mathematical Models and Methods in Applied Sciences2010，4，1： | 1 |
| 19 | Poliovirus 2A^pro induces the nucleic translocation of poliovirus 3CD and 3C' proteins显示文摘脊髓灰质炎病毒 genomic RNA 复制,蛋白质翻译,和 virion 汇编在主人房间的细胞质被执行。然而,这不意味着在脊髓灰质炎病毒感染和细胞的原子核之间没有关系。在这研究,标注荧光的脊髓灰质炎病毒 3CD 和 3C 蛋白质被显示出当另外的病毒的蛋白质不在时主要在 Vero 房间的细胞质被表示的 recombinant。在脊髓灰质炎病毒感染之上,然而,许多这些蛋白质再分配到原子核,以及到细胞质。一系列 transfection 实验表明脊髓灰质炎病毒 2Apro 为 3CD 和 3C 蛋白质的一样的再分配负责到原子核。而且,缺乏朊酶活动的变异的 2Apro 蛋白质废除了这效果。脊髓灰质炎病毒 2Apro 蛋白质也被发现到与 Nup153 蛋白质共同本地化,原子信封上的原子毛孔建筑群的一个部件。这些数据提供在脊髓灰质炎病毒蛋白质和房间原子核之间有内在的相互作用的进一步的证据,尽管有那,在脊髓灰质炎病毒复制周期的许多进程发生在细胞质。 | Wenwu Tian Zongqiang Cui Zhiping Zhang Hongping Wei XianEn Zhang | 2011 | Acta Biochimica et Biophysica Sinica2011，43，1： | 1 |
| 20 | Folding beam-type piezoelectric phononic crystal with low-frequency and broad band gap显示文摘A folding beam-type piezoelectric phononic crystal model is proposed to isolate vibration. Two piezoelectric bimorphs are joined by two masses as a folding structure to comprise each unit cell of the piezoelectric phononic crystal. Each bimorph is connected independently by a resistive-inductive resonant shunting circuit. The folding structure extends the propagation path of elastic waves, while its structure size remains quite small. Propagation of coupled extension-flexural elastic waves is studied by the classical laminated beam theory and transfer matrix method. The theoretical model is further verified with the finite element method(FEM). The effects of geometrical and circuit parameters on the band gaps are analyzed. With only 4 unit cells, the folding beam-type piezoelectric phononic crystal generates two Bragg band gaps of 369 Hz to1 687 Hz and 2 127 Hz to 4 000 Hz. In addition, between these two Bragg band gaps, a locally resonant band gap is induced by resonant shunting circuits. Appropriate circuit parameters are used to join these two Bragg band gaps by the locally resonant band gap.Thus, a low-frequency and broad band gap of 369 Hz to 4 000 Hz is obtained. | Shan JIANG Longxiang DAI Hao CHEN Hongping HU Wei JIANG Xuedong CHEN | 2017 | Applied Mathematics and Mechanics(English Edition)2017，38，3： | 1 |
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