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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | 新版Bethesda指南、微卫星不稳定性检测和免疫组化检查诊断遗传性非息肉性结直肠癌的准确性显示文摘背景:挑选适当个体进行遗传性非息肉性结直肠癌(hereditary nonpolyposis colorectal cancer,HNPCC)基因检测有一定难度。最近,美国国立癌症研究所(National Cancer Institute)在新版Bethesda指南中给出了一套新的推荐方案,以明确哪些HNPCC个体应行微卫星不稳定性检测。 | Virginia Pinol, MD Antoni Castells, MD Montserrat Andreu, MD 许峰(译) 祝学光(校) | 2005 | 美国医学会杂志(中文版)2005，24，6： | 23 |
| 3 | Patients with irritable bowel syndrome-diarrhea have lower disease-specific quality of life than irritable bowel syndrome-constipation显示文摘AIM: To determine effect of irritable bowel syndrome(IBS) subtype on IBS-specific quality of life(QOL) questionnaire and its subscales.METHODS: We studied IBS patients visiting our functional gastroenterology disorder clinic at a tertiary care center of Unites States.IBS and IBS subtype were diagnosed using Rome-Ⅲ questionnaire.QOL was assessed using IBS-QOL questionnaire.IBSQOL assesses quality of life along eight subscales: dysphoria,interference with activities,body image,health worry,food avoidance,social reactions,sexual health,and effect on relationships.IBS-QOL and its subscales were both scored on a range of 0-100 with higher scores suggestive of better QOL.Results of overall IBS-QOL scores and subscale scores are expressed as means with 95%CI.We compared mean IBS-QOL score and its subscales among various IBSsubtypes.Analysis of variance(ANOVA) was used to compare the mean difference between more than two groups after controlling for age and gender.A posthoc analysis using Bonferroni correction was used only when P value for ANOVA was less than 0.05.RESULTS: Of 542 patients screened,243 had IBS as per Rome-Ⅲ criteria.IBS-mixed(IBS-M) was the most common IBS subtype(121 patients,49.8%) followed by IBS- diarrhea(IBS-D)(56 patients,23.1%),IBSconstipation(IBS-C)(54 patients,22.2%) and IBSunspecified(IBS-U)(12 patients,4.9%).Overall IBSQOL scores were significantly different among various IBS-subtypes(P = 0.01).IBS-QOL of patients with IBS-D(61.6,95%CI: 54.0-69.1) and IBS-M(63.0,95%CI: 58.1-68.0) was significantly lower than patients with IBS-C(74.5,95%CI: 66.9-82.1)(P = 0.03 and 0.02 respectively).IBS-D patients scored significantly lower than IBS-C on food avoidance(45.0,95%CI: 34.8-55.2 vs 61.1,95%CI: 50.8-71.3,P = 0.04) and interference with activity(59.6,95%CI: 51.4-67.7 vs 82.3,95%CI: 74.1-90.6,P < 0.001).IBS-M patients had more interference in their activities(61.6,95%CI: 56.3-66.9 vs 82.3,95%CI: 74.1-90.6,P = 0.001) and greater impact on their relationships(73.3,95%CI: 68.4-78.2 vs 84.7,95%CI: 77.2-92.2,P = 0.02) than IBS-C patients.Patients with IBS-M also scored significantly lower than IBS-C on food avoidance(47.2,95%CI: 40.7-53.7 vs 61.1,95%CI: 50.8-71.3,P = 0.04) and social reaction(66.1,95%CI: 61.1-71.1 vs 80.0,95%CI: 72.1-87.7,P = 0.005).CONCLUSION: IBS-D and IBS-M patients have lower IBS-QOL than IBS-C patients.Clinicians should recognize food avoidance,effects on daily activities and relationship problems in these patients. | Prashant Singh Kyle Staller Kenneth Barshop Elaine Dai Jennifer Newman Sonia Yoon Shahar Castel Braden Kuo | 2015 | World Journal of Gastroenterology2015，21，26： | 9 |
| 4 | Adjuvant therapy sparing in rectal cancer achieving complete response after chemoradiation显示文摘AIM:To evaluate the long-term results of conventional chemoradiotherapy and laparoscopic mesorectal excision in rectal adenocarcinoma patients without adjuvant therapy.METHODS:Patients with biopsy-proven adenocarcinoma of the rectum staged cT3-T4 by endoscopic ultrasound or magnetic resonance imaging received neoadjuvant continuous infusion of 5-fluorouracil for five weeks and concomitant radiotherapy.Laparoscopic surgery was planned after 5-8 wk.Patients diagnosed with ypT0N0 stage cancer were not treated with adjuvant therapy according to the protocol.Patients with ypT1-2N0 or ypT3-4 or N+were offered 5-fluorouracil-based adjuvant treatment on an individual basis.An external cohort was used as a reference for the findings.RESULTS:One hundred and seventy six patients were treated with induction chemoradiotherapy and 170underwent total mesorectal excision.Cancer staging of ypT0N0 was achieved in 26/170(15.3%)patients.After a median follow-up of 58.3 mo,patients withypT0N0 had five-year disease-free and overall survival rates of 96%(95%CI:77-99)and 100%,respectively.We provide evidence about the natural history of patients with localized rectal cancer achieving a complete response after preoperative chemoradiation.The inherent good prognosis of these patients will have implications for clinical trial design and care of patients.CONCLUSION:Withholding adjuvant chemotherapy after complete response following standard neoadjuvant chemoradiotherapy and laparoscopic mesorectal excision might be safe within an experienced multidisciplinary team. | Xabier García-Albéniz Rosa Gallego Ralf Dieter Hofheinz Gloria Fernández-Esparrach Juan Ramón Ayuso-Colella Josep Antoni Bombí Carles Conill Miriam Cuatrecasas Salvadora Delgado Angels Ginés Rosa Miquel Mario Pagés Estela Pineda Verónica Pereira Aarón Sosa Oscar Reig Iván Victoria Luis Feliz Antonio María de Lacy Antoni Castells Iris Burkholder Andreas Hochhaus Joan Maurel | 2014 | World Journal of Gastroenterology2014，20，42： | 5 |
| 5 | 不同来源粗饲料对荷斯坦犊牛生长性能及采食行为的影响显示文摘选用179头荷斯坦公犊牛(体重44.7kg、日龄8.3d)参与3个试验,用以评价不同来源粗饲料对其生长性能、表观消化率和采食行为的影响。在每个试验中,犊牛均被分为3组,对照组(CON)犊牛饲喂开食料,不添加任何粗饲料,其他2个处理组饲喂相同的开食料并各添加1种粗饲料:第1个试验中添加切碎的苜蓿(AH)或黑麦干草(RH);第2个试验中添加燕麦干草(OH)或大麦秸秆(BS);第3个试验中添加玉米青贮(CS)或小黑麦青贮(TS)。所有犊牛通过奶瓶饲喂2L代乳料,含量占干物质含量的12.5%,每日饲喂2次,直到日龄第50天。断奶前1周内同样饲喂2L代乳料,含量占干物质含量的12.5%(57d前)。试验在犊牛日龄达到71d时结束,每日记录开食料、代乳料和粗饲料采食量,每周测定犊牛体重。犊牛单圈饲养,且牛床上铺有刨花。结果表明,与对照组相比,添加OH、TS和BS组犊牛开食料采食量升高(0.88vs.1.14、1.17、1.06kg/d),且日增重显著提高(0.72vs.0.93、0.88、0.88kg/d)。饲喂RH、BS、CS和TS组犊牛粗饲料采食量(51g/d)低于AH(120g/d)和OH组(101g/d)。各处理组有机物、干物质和中性洗涤纤维的表观消化率无显著差异(分别为81.5%、81.1%、54.4%)。RH、CS和AH组粗蛋白质的表观消化率高于对照组(80.5%vs.76.4%)。与对照组相比,AH组犊牛开食料采食量下降,躺卧的时间较短,AH和RH组犊牛反刍时间延长,优势比(OR)分别为5.24和5.40,且AH和RH组犊牛进行非营养性口腔行为所耗费的时间较少(OR分别为0.38和0.34)。TS组犊牛在饲喂代乳料和固体饲料后1h内进行非营养性口腔行为的时间有缩短的趋势(OR:0.21)。总之,以自由选择的方式给犊牛供应不同来源的粗饲料可以提高采食量和生长性能,且不影响干物质、有机物、粗蛋白质和中性洗涤纤维的表观消化率;同时,不同来源的粗饲料还可以减少非营养性口腔行为并刺激反刍。 | Castells 孙鹏 | 2013 | 中国畜牧兽医2013，40，2： | 4 |
| 6 | New genes emerging for colorectal cancer predisposition显示文摘Colorectal cancer(CRC)is one of the most frequent neoplasms and an important cause of mortality in the developed world.This cancer is caused by both genetic and environmental factors although 35%of the variation in CRC susceptibility involves inherited genetic differences.Mendelian syndromes account for about5%of the total burden of CRC,with Lynch syndrome and familial adenomatous polyposis the most common forms.Excluding hereditary forms,there is an important fraction of CRC cases that present familial aggregation for the disease with an unknown germline genetic cause.CRC can be also considered as a complex disease taking into account the common diseasecommom variant hypothesis with a polygenic model of inheritance where the genetic components of common complex diseases correspond mostly to variants of low/moderate effect.So far,30 common,low-penetrance susceptibility variants have been identified for CRC.Recently,new sequencing technologies including exomeand whole-genome sequencing have permitted to add a new approach to facilitate the identification of new genes responsible for human disease predisposition.By using whole-genome sequencing,germline mutations in the POLE and POLD1 genes have been found to be responsible for a new form of CRC genetic predisposition called polymerase proofreading-associated polyposis. | Clara Esteban-Jurado Pilar Garre Maria Vila Juan José Lozano Anna Pristoupilova Sergi Beltrán Anna Abulí Jenifer Muoz Francesc Balaguer Teresa Ocaa Antoni Castells Josep M Piqué Angel Carracedo Clara Ruiz-Ponte Xavier Bessa Montserrat Andreu Luis Bujanda Trinidad Caldés Sergi Castellví-Bel | 2014 | World Journal of Gastroenterology2014，20，8： | 3 |
| 7 | Hepatocyte transplantation program:Lessons learned andfuture strategies显示文摘This review aims to share the lessons we learned over time during the setting of the hepatocyte transplantation(HT) program at the Hepatic Cell Therapy Unit at Hospital La Fe in Valencia. New sources of liver tissue for hepatocyte isolation have been explored. The hepatocyte isolation and cryopreservation procedures have been optimized and quality criteria for assessment of functionality of hepatocyte preparations and suitability for HT have been established. The results indicate that:(1) Only highly viable and functional hepatocytes allow to recover those functions lacking in the native liver;(2) Organs with steatosis(≥ 40%) and from elderly donors are declined since low hepatocyte yields, viability and cell survival after cryopreservation, are obtained;(3) Neonatal hepatocytes are cryopreserved without significant loss of viability or function representing high-quality cells to improve human HT;(4) Cryopreservation has the advantage of providing hepatocytes constantly available and of allowing the quality evaluation and suitability for transplantation; and(5) Our results from 5 adults with acute liver failure and 4 from children with inborn metabolic diseases, indicate that HT could be a veryuseful and safe cell therapy, as long as viable and metabolically functional human hepatocytes are used. | Eugenia Pareja Ibars Miriam Cortes Laia Tolosa Maria JoséGómez-Lechón Slivia López JoséVicente Castell JoséMir | 2016 | World Journal of Gastroenterology2016，22，2： | 3 |
| 8 | Surgical resection of hepatocellular carcinoma in cirrhotic patients: Prognostic value of preoperative portal pressure显示文摘 | J Bruix A Castells J Bosch F Feu J Fuster JC Garcia-Pagan J Visa C Bru J Rodes | 1996 | Gastroenterology1996，，4： | 3 |
| 9 | Laparoscopy-assisted colectomy versus open colectomy for treatment of non-metastatic colon cancer: a randomised trial显示文摘 | Antonio M Lacy Juan C García-Valdecasas Salvadora Delgado Antoni Castells Pilar Taurá Josep M Piqué Josep Visa | 2002 | 2002 (9325)2002，，9325： | 3 |
| 10 | Cardiac alterations in cirrhosis: reversibility after liver transplantation显示文摘 | Mireia Torregrosa Santi Aguadé Laura Dos Rosa Segura Antonio Gónzalez Artur Evangelista Joan Castell Carlos Margarit Rafael Esteban Jaume Guardia Joan Genescà | 2004 | Journal of Hepatology2004，，1： | 3 |
| 11 | Identification of Lynch syndrome: How should we proceed in the 21^(st) century?显示文摘Lynch syndrome, also known as hereditary non-polyposis colorectal cancer (HNPCC), is the most common form of hereditary colorectal cancer. Although great advances in the understanding of its molecular basis have taken place in the last decade, optimal selection of individuals for HNPCC genetic testing remains controversial. This is especially relevant since colonoscopy has been proven effective for reducing colorectal cancer incidence and mortality in individuals at-risk for this disorder. In this manuscript, we summarize the most significant contributions to this important issue that have appearedin the last few years. | Antoni Castells Francesc Balaguer Sergi Castellví-Bel Victòria Gonzalo Teresa Ocaa | 2007 | World Journal of Gastroenterology2007，13，33： | 3 |
| 12 | Laparoscopy-assisted colectomy versus open colectomy for treatment of non-metastatic colon cancer: a randomised trial显示文摘 | Antonio M Lacy Juan C García-Valdecasas Salvadora Delgado Antoni Castells Pilar Taurá Josep M Piqué Josep Visa | 2002 | The Lancet2002，，9325： | 2 |
| 13 | 紫杉醇和多西紫杉醇超敏反应的快速脱敏疗法:一种成功应用于77例患者的新标准方案 | Feldweg A.M. Lee C.- W. Matulonis U.A. Castells M. 刘秀娟 | 2005 | 世界核心医学期刊文摘(妇产科学分册)2005，0，7： | 2 |
| 14 | Evaluation of microdiets versus live feeds on growth, survival and fatty acid composition of larval haddock ( Melanogrammus aeglefinus )显示文摘 | Tammy Blair John Castell Steven Neil Louis D’Abramo Chantal Cahu Paul Harmon Kehinde Ogunmoye | 2003 | Aquaculture2003，，1： | 2 |
| 15 | The Long-term Results of a Randomized Clinical Trial of Laparoscopy-assisted Versus Open Surgery for Colon Cancer显示文摘 | Antonio M. Lacy Salvadora Delgado Antoni Castells Hubert A. Prins Vicente Arroyo Ainitze Ibarzabal Josep M. Pique | 2008 | Annals of Surgery2008，，1： | 2 |
| 16 | Diagnostic efficacy of gadoxetic acid (Primovist)-enhanced MRI and spiral CT for a therapeutic strategy: comparison with intraoperative and histopathologic findings in focal liver lesions显示文摘 | Renate Hammerstingl Alexander Huppertz Josy Breuer Thomas Balzer Anthony Blakeborough Rick Carter Lluis Castells Fusté Gertraud Heinz-Peer Werner Judmaier Michael Laniado Riccardo M. Manfredi Didier G. Mathieu Dieter Müller Koenraad Mortelè Peter Reimer M | 2008 | European Radiology2008，，： | 2 |
| 17 | 不同来源粗饲料对荷斯坦犊牛生长性能和摄食行为的影响显示文摘179头荷斯坦公犊牛(体重44.7kg,日龄8.3d)参与3个试验,用以评价不同来源粗饲料对其生长性能、表观消化率和摄食行为的影响。在每项研究中犊牛均被分为3个不同日粮处理组:对照组(CON)犊牛饲喂开食料,不添加任何粗饲料(这一处理组在3个试验中重复使用),其他2个处理组饲喂相同的开食料各添加一种粗饲料:第1个试验中添加切碎的苜蓿(AH)或黑麦干草(RH);第2个试验中添加燕麦干草(OH)或大麦秸秆(BS);第3个试验中添加玉米青贮(CS)或小黑麦青贮(TS)。所有犊牛通过奶瓶饲喂2L代乳料,相当于干物质含量的12.5%,每日饲喂2次,直到50日龄。断奶前1周内同样饲喂2L代乳料,含量占干物质含量的12.5%(57d前)。试验在犊牛日龄达到71d时结束。每日记录开食料、代乳料和粗饲料采食量,每周测定犊牛体重。犊牛单圈饲养,牛床上铺有刨花。与对照组相比,添加OH、TS和BS组犊牛开食料采食量升高(0.88对1.14、1.17、1.06kg/d),且日增重显著提高(0.72对0.93、0.88、0.88kg/d)。饲喂RH、BS、CS和TS组犊牛粗饲料采食量(51g/d)低于AH(120g/d)和OH组(101g/d)。各处理组有机物、干物质和中性洗涤纤维的表观消化率无差异(分别为81.5%、81.1%、54.4%)。RH、CS和AH组粗蛋白的表观消化率高于对照组(80.5%对76.4%)。与对照组相比,AH组犊牛开食料采食量下降,躺卧的时间较短,AH和RH组犊牛反刍时间延长,优势比(OR)分别为5.24和5.40。且AH和RH组犊牛进行非营养性口腔行为所耗费的时间较少(OR分别为0.38、0.34)。TS组犊牛在饲喂代乳料和固体饲料后1h内进行非营养性口腔行为的时间有缩短的趋势(OR:0.21)。总之,以自由选择的方式给犊牛供应不同来源的粗饲料可以提高采食量和生长性能,且不影响干物质、有机物、粗蛋白和中性洗涤纤维的表观消化率。同时,不同来源的粗饲料还可以减少非营养性口腔行为并刺激反刍。 | Castells 孙鹏 | 2012 | 中国畜牧兽医2012，39，9： | 2 |
| 18 | Liver Retransplantation in HIV‐Infected Patients: A Prospective Cohort Study显示文摘 | M. Gastaca F. Aguero A. Rimola M. Montejo P. Miralles R. Lozano L. Castells M. Abradelo M. de la Mata F. San Juan Rodríguez E. Cordero S. del Campo C. Manzardo J.O. de Urbina I. Pérez G. de la Rosa J. M. Miro | 2012 | American Journal of Transplantation2012，，9： | 2 |
| 19 | Outcome of HCV/HIV‐Coinfected Liver Transplant Recipients: A Prospective and Multicenter Cohort Study显示文摘 | J. M. Miro M. Montejo L. Castells A. Rafecas S. Moreno F. Agüero M. Abradelo P. Miralles J. Torre‐Cisneros J. D. Pedreira E. Cordero G. de la Rosa B. Moyano A. Moreno I. Perez A. Rimola | 2012 | American Journal of Transplantation2012，，7： | 2 |
| 20 | Effects of purified dies containing different combinations of arachidonic and docosahexaenoic acid on survival, growth and fatty acid composition of juvenile turbot (Scophthalmus maximus) 显示文摘 | Castell J D Bell J G Tocher D R | 1994 | Aquaculture1994，1，： | 2 |
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