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| 1 | Pathological abnormalities in splenic vasculature in non-cirrhotic portal hypertension:Its relevance in the management of portal hypertension显示文摘BACKGROUND Portal hypertension(PH)is associated with changes in vascular structure and function of the portosplenomesenteric system(PSMS).This is referred to as portal hypertensive vasculopathy.Pathological abnormalities of PSMS has been described in the literature for cirrhotic patients.Raised portal pressure and hyperdynamic circulation are thought to be the underlying cause of this vasculopathy.In view of this,it is expected that pathological changes in splenic and portal vein similar to those reported in cirrhotic patients with PH may also be present in patients with non-cirrhotic PH(NCPH).AIM To investigate pathological abnormalities of splenic vein in patients with NCPH,and suggest its possible implications in the management of PH.METHODS A prospective observational study was performed on 116 patients with NCPH[Extrahepatic portal vein obstruction(EHPVO):53 and non-cirrhotic portal fibrosis(NCPF):63]who underwent proximal splenorenal shunt(PSRS),interposition shunt or splenectomy with devascularization in JIPMER,Pondicherry,India,a tertiary level referral center,between 2011-2016.All patients were evaluated by Doppler study of PSMS,computed tomography portovenogram and upper gastrointestinal endoscopy.An acoustic resonance forced impulse(ARFI)scan and abdomen ultrasound were done for all cases to exclude cirrhosis.Intraoperative and histopathological assessment of the harvested splenic vein was performed in all.The study group was divided into delayed and early presentation based on the median duration of symptoms(i.e.108 mo).RESULTS The study group comprising of 116 patients[77(66%)females and 39(34%)males]with NCPH had a median age of 22 years.Median duration of symptoms was 108 mo.The most common presentation in both EHPVO and NCPF patients was upper gastrointestinal bleeding(hematemesis and melena).The ARFI scan revealed a median score of 1.2(1.0-1.8)m/s for EHPVO and 1.5(0.9-2.8)m/s for NCPF.PSRS was performed in 84 patients(two of whom underwent interposition PSRS using a 10 mm Dacron graft);splenoadrenal shunt in 9;interposition mesocaval shunt in 5;interposition 1st jejunal to caval shunt in 1 patient and devascularization with splenectomy in 17 patients.Median presplenectomy portal pressure was 25(range:15-51)mm Hg.In 77%cases,the splenic vein was abnormal upon intraoperative assessment.Under macroscopic examination,wall thickening was observed in 108(93%),venous thrombosis in 32(28%)and vein wall calcification in 27(23%)cases.Upon examination under a surgical magnification loupe,21(18%)patients had intimal defects in the splenic vein.Histopathological examination of veins was abnormal in all cases.Medial hypertrophy was noted in nearly all patients(107/116),while intimal fibrosis was seen in 30%.Ninety one percent of patients with intimal fibrosis also had venous thrombosis.Vein wall calcification was found in 22%,all of whom had intimal fibrosis and venous thrombosis.The proportion of patients with pathological abnormalities in the splenic vein were significantly greater in the delayed presentation group as compared to the early presentation group.CONCLUSION Pathological changes in the splenic vein similar to those in cirrhotic patients with PH are noted in NCPH.We recommend that PH in NCPH be treated as systemic and pulmonary hypertension equivalent in the gastrointestinal tract,and that early aggressive therapy be initiated to reduce portal pressure and hemodynamic stress to avoid potential lethal effects. | Shahana Gupta Biju Pottakkat Surendra Kumar Verma Raja Kalayarasan Sandip Chandrasekar A Ajith Ananthakrishna Pillai | 2020 | World Journal of Gastrointestinal Surgery2020，12，1： | 3 |
| 2 | Efficient Gene Targeting in Maize Using Inducible CRISPR-Cas9 and Marker-free Donor Template显示文摘CRISPR-Cas9 is a powerful tool for generating targeted mutations and genomic deletions.However,precise gene insertion or sequence replacement remains a major hurdle before application of CRISPR-Cas9 technology is fully realized in plant breeding.Here,we report high-frequency,selectable marker-free intra-genomic gene targeting(GT)in maize.Heat shock-inducible Cas9 was used for generating targeted double-strand breaks and simultaneous mobilization of the donor template from pre-integratad T-DNA.The construct was designed such that release of the donor template and subsequent DNA repair activated expression of the selectable marker gene within the donor locus.This approach generated up to 4.7%targeted insertion of the donor sequence into the target locus in TO plants,with up to 86%detected donor template release and 99%mutation rate being observed at the donor loci and the genomic target site,respectively.Unlike previous in planta or intra-genomic homologous recombination reports in which the original chimeric GT plants required extensive progeny screening in the next generation to identify non-chimeric GT individuals,our method provides non-chimeric heritable GT in one generation. | Pierluigi Barone Emily Wu Brian Lenderts Ajith Anand William Gordon-Kamm Sergei Svitashev Sandeep Kumar | 2020 | Molecular Plant2020，13，8： | 3 |
| 3 | Antibacterial potential of sponge endosymbiont marine Enterobacter sp at Kavaratti Island,Lakshadweep archipelago显示文摘Objective:To isolate antibacterial potential of sponge endosymbiotic bacteria from marine sponges at Lakshadweep archipelago.Also to identify the potent bacteria by 16s rDNA sequencing and determine the antibacterial activity against clinical pathogens by MIC.Methods: Sponge samples was collected from sub-tidal habitats at Kavaratti Island and identified.The endosymbiotic bacteria were isolated and selected potential bacteria which show antibacterial activity in preliminary screening against clinical pathogens Staphylococcus aureus(S.aureus), Escherichia coli(E.coli),Salmonella typhi(S.typhi),Klebsiella pneumoniea(K.pneumoniea) and Streptococcus sp.by disc diffusion assay.The crude extracts of potential bacteria LB3 was tested against clinical pathogens by MIC.The LB3 strain was identified by 16s rDNA sequencing, 1111 bp was submitted in NCBI(HQ589912) and constructed phylogenetic tree.Results:Sponge sample was identified as Dysidea granulosa(D.granulosa) and potential bacteria LB3 identified as Enterobacter sp TTAG.Preliminary screening of sponge isolates against clinical pathogens,LB3 strain was selected as potential producer of secondary metabolites and crude extract was implies on MIC of LB3 have confirmed with lowest concentration of 5.0 mg/mL in broth medium influence of crude extract on growth inhibitory activity after 5 h of incubation period and completed the inhibitory activity at 15 h.Conclusions:The present study concluded that phylogenetic analysis of endosymbiotic bacteria Enterobacter sp from sponge D.granulosa of Lakshadweep islands showed significant antibacterial activity against clinical bacterial pathogens. | Mohan Gopi Subramanian Kumaran Thipparamalai Thangappanpillai Ajith Kumar Balaraman Deivasigamani Kumarappan Alagappan Srinivasan Guru Prasad | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，2： | 2 |
| 4 | Influencing R&D/marketing integration and the use of market information by R&D managers:Intended and unintended effects of managerial actions显示文摘 | Elliot Maltz William E Sounder Ajith Kumar | 2001 | Journal of Business Research2001，52，： | 1 |
| 5 | Embryonic development of percula clown fish,Amphiprio percula(Lacepede,1802)显示文摘 | DHANEESH K V AJITH KUMAR T T SHNUMUGARAJ T | 2009 | Middle-East Journal of Scientific Research2009，4，2： | 1 |
| 6 | Recurrent craniospinal subarachnoid hemorrhage in cerebral amyloid angiopathy显示文摘 | Mathew Alexander Anil Kumar Patil Vivek Mathew Ajith Sivadasan Geeta Chacko Sunithi Mani | 2013 | Annals of Indian Academy of Neurology2013，，1： | 1 |
| 7 | High upconversion quantum yield at low pump threshold in Er 3+ /Yb 3+ doped La 2 O 2 S phosphor显示文摘 | Madhab Pokhrel Ajith kumar Gangadharan Dhiraj K. Sardar | 2013 | Materials Letters2013，，： | 1 |
| 8 | Breeding embryonic development and salinity tolerance of Skunk clownfish Amphiprion akallopisos 显示文摘 | DHANEESH K V NANTHINI DEVIR K AJITH KUMAR T T | 2012 | Journal of King Saud University Science2012，，24： | 1 |
| 9 | Changes in Solidification Morphology of Mg–Si Alloys by Ca Additions显示文摘 | K. K. Ajith Kumar Abhilash Viswanath U. T. S. Pillai B. C. Pai M. Chakraborty | 2012 | Transactions of the Indian Institute of Metals2012，，6： | 1 |
| 10 | Bidirectional crosstalk between periventricular endothelial cells and neural progenitor cells promotes the formation of a neurovascular unit显示文摘 | Ravi Vissapragada Mauricio A. Contreras Cleide G da Silva Vivek A. Kumar Angelica Ochoa Anju Vasudevan Magdy H. Selim Christiane Ferran Ajith J. Thomas | 2014 | Brain Research2014，，： | 1 |
| 11 | A very useful tool in analyzing flow - induced vibration data 显示文摘 | Ajith Kumar P Sugumaran V Gowda B H L Sohn C H | 2008 | Mechanical Systems and Signal processing2008，22，： | 1 |
| 12 | RFID-based product information in end-of-life decision making 显示文摘 | Ajith Kumar Parlikad Duncan McFarlane | 2007 | Control Engineering Practice2007，，15： | 1 |
| 13 | Dry Sliding Wear Behaviour of Mg-Si Alloys显示文摘 | Kumar Ajith K K Pillai U T S Pai B C | 2013 | Wear2013，303，： | 1 |
| 14 | Recurrent craniospinal subarachnoid hemorrhage in cerebral amyloid angiopathy显示文摘 | Mathew Alexander Anil Kumar Patil Vivek Mathew Ajith Sivadasan Geeta Chacko Sunithi Mani | 2013 | Annals of Indian Academy of Neurology2013，，1： | 1 |
| 15 | Market Orientation and Alternative Strategic Orientations: A Longitudinal Assessment ofeompetitiveness Implications 显示文摘 | Charles H Noble Rajiv K Sinha Ajith Kumar | 2003 | Journal of Marketing2003，10，： | 1 |
| 16 | Breeding and mass scale rearing of clownfish Amphiprion percula: feeding and rearing in brackishwater显示文摘Breeding and mass scale larval rearing of clownfish Amphiprion percula is very limited in brackishwater. We designed an indoor program of A. percula culture in brackishwater with a salinity of 24±1, during which the impacts of feed type, water temperature, and light intensity, on the efficiency of its reproduction, were revealed. The fish were accommodated along with sea anemones in fibre glass tanks to determine the influence of brooder diet on breeding efficiency. Higher reproductive efficiency [number of eggs laid (276 22.3 eggs)] was observed when fish were fed live Acetes sp. rather than clam (204 16.4 eggs), trash fish (155 12 eggs) and formulated feed (110 10 eggs). The spawning rate was increased during September and October (water temperature, 28.74 0.55°C) on average of 2.4 spawning per month; and low spawning rate was in January (water temperature, 24.55 0.45°C) on average of 1 spawning per month. Among three light intensities (100, 500, and 900 lx) set to evaluate larval survival rate, larvae showed the highest survival rate (65.5%) at 900 lx. The breeding method specifically in brackishwater developed in the present study is a new approach, will help the people from the regions of estuary and backwater to enhance their livelihood and it will lead to reduce the exploitation from the wild habitat. | DHANEESH Kottila Veettil AJITH KUMAR Thipramalai Thankappan SWAGAT Ghosh BALASUBRAMANIAN Thangavel | 2012 | Chinese Journal of Oceanology and Limnology2012，30，4： | 1 |
| 17 | Enhancement of flow-induced motion of rigid circular cylinder on springs by localized surface roughness at 3×10 4 ≤ Re ≤1.2×10 5显示文摘 | Hongrae Park R. Ajith Kumar Michael M. Bernitsas | 2013 | Ocean Engineering2013，，： | 1 |
| 18 | A Retailed Study of Mobility Models in Wireless Sensor Network 显示文摘 | Vasanthi V Romen Kumar M Ajith Singh N | 2011 | Journal of Theoretical and Applied Information Technology2011，33，1： | 1 |
| 19 | VIV and galloping of single circular cylinder with surface roughness at 3.0×10 4 ≤ Re ≤1.2×10 5显示文摘 | Che-Chun (Jim) Chang R. Ajith Kumar Michael M. Bernitsas | 2011 | Ocean Engineering2011，，16： | 1 |
| 20 | A Measure of Market Orientation显示文摘 | KOHLI A K KUMAR AJITH MARKOR | 1993 | Journal of Marketing Research1993，，30： | 1 |
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