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| 1 | Proteomic survey towards the tissue-specific proteins of mouse mitochondria显示文摘Mitochondrion plays the key functions in mammalian cells. It is believed that mitochondrion exerts the common biologic functions in many tissues, but also performs some specific functions correspondent with tissues where it is localized. To identify the tissue-specific mitochondrial proteins, we carried out a systematic survey towards mitochondrial proteins in the tissues of C57BL/6J mouse, such as liver, kidney and heart. The mitochondrial proteins were separated by 2DE and identified by MALDI-TOF/TOF MS. Total of 87 unique proteins were identified as the tissue-specific ones, and some representatives were further verified through ICPL quantification and Western blot. Because these issue-specific proteins are coded from nuclear genes, real-time PCR was employed to examine the mRNA status of six typical genes found in the tissues.With combining of the expression data and the co-localization images obtained from confocal microscope, we came to the conclusion that the tissue-specifically mitochondrial proteins were widely distributed among the mouse tissues. Our investigation, therefore, indeed provides a solid base to further explore the biological significance of the mitochondrial proteins with tissue-orientation. | WANG Yuan SUN HaiDan RU YaWei YIN SongYue YIN Liang LIU SiQi | 2011 | Science China(Life Sciences)2011，54，1： | 3 |
| 2 | Long Noncoding RNA MALAT-1 is a New Potential Therapeutic Target for Castration-Resistant Prostate Cancer显示文摘 | Shancheng Ren Yawei Liu Weidong Xu Yi Sun Ji Lu Fubo Wang Min Wei Jian Shen Jianguo Hou Xu Gao Chuanliang Xu Jiaoti Huang Yi Zhao Yinghao Sun | 2013 | The Journal of Urology2013，，： | 2 |
| 3 | Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces显示文摘CRISPR/Cas-mediated genome editing has greatly facilitated the study of gene function in Streptomyces. However, it could not be efficiently employed in streptomycetes with low homologous recombination(HR) ability. Here, a deaminase-assisted base editor d Cas9-CDA-ULstr was developed in Streptomyces, which comprises the nuclease-deficient Cas9(dCas9), the cytidine deaminase from Petromyzon marinus(PmCDA1), the uracil DNA glycosylase inhibitor(UGI) and the protein degradation tag(LVA tag). Using d Cas9-CDA-ULstr , we achieved single-, double-and triple-point mutations(cytosine-to-thymine substitutions)at target sites in Streptomyces coelicolor with efficiency up to 100%, 60% and 20%, respectively. This base editor was also demonstrated to be highly efficient for base editing in the industrial strain, Streptomyces rapamycinicus, which produces the immunosuppressive agent rapamycin. Compared with base editors derived from the cytidine deaminase rAPOBEC1, the PmCDA1-assisted base editor dCas9-CDA-ULstr could edit cytosines preceded by guanosines with high efficiency, which is a great advantage for editing Streptomyces genomes(with high GC content). Collectively, the base editor dCas9-CDA-ULstr could be employed for efficient multiplex genome editing in Streptomyces. Since the d Cas9-CDA-ULstr -based genome editing is independent of HR-mediated DNA repair, we believe this technology will greatly facilitate functional genome research and metabolic engineering in Streptomyces strains with weak HR ability. | Yawei Zhao Jinzhong Tian Guosong Zheng Jun Chen Chuanwen Sun Zhongyi Yang Andrei A.Zimin Weihong Jiang Zixin Deng Zhijun Wang Yinhua Lu | 2020 | Science China(Life Sciences)2020，63，7： | 2 |
| 4 | Interleukin 6 protects H<sub>2</sub>O<sub>2</sub>‐induced cardiomyocytes injury through upregulation of prohibitin via STAT3 phosphorylation显示文摘 | Yuhua Jia Fenghua Zhou Peng Deng Qin Fan Chunhua Li Yawei Liu Xiuqiong Fu Yingchun Zhou Xin Xu Xuegang Sun | 2012 | Cell Biochem Funct2012，，5： | 1 |
| 5 | Influence of nano-scale twins (NT) structure on passive film formed on nickel显示文摘 | Guozhe Meng Feilong Sun Yawei Shao Tao Zhang Fuhui Wang Chaofang Dong Xiaogang Li | 2009 | Electrochimica Acta2009，，7： | 1 |
| 6 | Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats显示文摘 | Jing Tang Yijuan Sun William Ka Kei Wu Tianyu Zhong Yawei Liu Jinfang Xiao Tao Tao Zhenlong Zhao Miaoning Gu | 2010 | BBA - Proteins and Proteomics2010，，9： | 1 |
| 7 | A new C=C embedded porphyrin sheet with superior oxygen reduction performance显示文摘C2 is a well-known pseudo-oxygen unit with an electron affinity of 3.4 eV. We show that it can exhibit metal-ion like behavior when embedded in a porphyrin sheet and form a metal-free two-dimensional material with superior oxygen reduction performance. Here, the positively charged C=C units are highly active for oxygen reduction reaction (ORR) via dissociation pathways with a small energy barrier of 0.09 eV, much smaller than that of other non-platinum group metal (non-PGM) ORR catalysts. Using a microkinetics-based model we calculated the partial current density to be 3.0 mA/cm2 at 0.65 V vs. a standard hydrogen electrode (SHE), which is comparable to that of the state-of-the-art Pt/C catalyst. We further confirm that the C=C embedded porphyrin sheet is dynamically and thermally stable with a quasi-direct band gap of 1.14 eV. The superior catalytic performance and geometric stability make the metal-free C=C porphyrin sheet ideal for fuel cell applications. | Yawei Li Shunhong Zhang Jiabing Yu Qian Wang Qiang Sun Puru Jena | 2015 | Nano Research2015，8，9： | 1 |
| 8 | Improved performance and air stability of perovskite solar cells based on low-cost organic hole-transporting material X60 by incorporating its dicationic salt显示文摘The development of an efficient, stable, and low-cost hole-transporting material (HTM) is of great significance for perovskite solar cells (PSCs) from future commercialization point of view. Herein, we specifically synthesize a dicationic salt of X60 termed X60(TFSI)2, and adopt it as an effective and stable 'doping' agent to replace the previously used lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) for the low-cost organic HTM X60 in PSCs. The incorporation of this dicationic salt significantly increases the hole conductivity of X60 by two orders of magnitude from 10-6 to 10-4 S cm-1. The dramatic enhancement of the conductivity leads to an impressive power conversion efficiency (PCE) of 19.0% measured at 1 sun illumination (100 mW cm-2, AM 1.5 G), which is comparable to that of the device doped with LiTFSI (19.3%) under an identical condition. More strikingly, by replacing LiTFSI, the PSC devices incorporating X60(TFSI)2 also show an excellent long-term durability under ambient atmosphere for 30 days, mainly due to the hydrophobic nature of the X60(TFSI)2 doped HTM layer,which can effectively prevent the moisture destroying the perovskite layer. The present work paves the way for the development of highly efficient, stable, and low-cost HTM for potential commercialization of PSCs. | Jishuang Qu Xiaoqing Jiang Ze Yu Jianbo Lai Yawei Zhao Maowei Hu Xichuan Yang Licheng Sun | 2018 | Science China Chemistry2018，61，2： | 1 |
| 9 | Number of Negative Lymph Nodes is Associated with Survival in Thoracic Esophageal Squamous Cell Carcinoma Patients Undergoing Three-Field Lymphadenectomy显示文摘 | Zhengfei Zhu Haiquan Chen Weiwei Yu Xiaolong Fu Jiaqing Xiang Hecheng Li Yawei Zhang Menghong Sun Qiao Wei Weixin Zhao Kuaile Zhao | 2014 | Annals of Surgical Oncology2014，，9： | 1 |
| 10 | Comparison of three data mining methods in predicting 5-year survival of colorectal cancer patients显示文摘The prediction of colorectal cancer(CRC) survivability has always been a challenging research issue. Considering the importance of predicting CRC patients' survival rates, we compared the performance of three data mining methods: decision trees(DTs), artificial neural networks(ANNs) and support vector machines(SVMs), for predicting 5-year survival of CRC patients to assist clinicians in making treatment decisions. The CRC dataset used to build the prediction model comes from the surveillance, epidemiology, and end results(SEER) program. The 5-fold cross-validation and random forest algorithm were respectively utilized for measuring the model predictive accuracy and the importance of features. Experimental results show that the predictive accuracy of ANNs(0.73) and SVMs(0.75) were higher than that of DTs, and they also have the best result in the area under the receiver operating characteristic(ROC) curve(area under curve(AUC)=0.82). This result may indicate high predictive power of ANNs and SVMs for predicting 5-year survival of CRC patients. | Luo Yan Sun Yawei Fu Qunchao Xue Tengfei Zhou Ping | 2018 | The Journal of China Universities of Posts and Telecommunications2018，25，6： | 0 |
| 11 | Effects of degenerative autologous nucleus pulposus on the lumbar sympathetic trunk: Sympathetic inflammation in rats显示文摘Objective: We have reported the presence of discogenic visceral pain secondary to anterior herniation of the lumbar disc. The aim of this study was to observe the inflammatory response of the sympathetic trunk to an autologous degenerative nucleus pulposus(NP) injection under fluoroscopy. Methods: A total of 72 rats were used. In 24 rats,the autologous NP suspension was injected into the right sympathetic trunk. Next,food intake and body weight of each rat were monitored for 14 days. Fourteen days after the injection,the right lumbar sympathetic trunk was harvested for histological assessment,and protein levels of IL-1 β,IL-6,and TNF-α were quantified via ELISA. Results: In the NP-treated group,endoneural hyperemia and intensive infiltration of inflammatory cells could be identified in sections of the sympathetic trunk by the hematoxylin and eosin(H&E) stain. Meanwhile,elevated concentrations of IL-1 β,IL-6,and TNF-α were determined in the sympathetic trunk of the NP group as compared to that of the na?ve and control groups,which indicated the development of an inflammatory response. Furthermore,food intake and body weight of rats in the NP group decreased significantly. Conclusions: The results indicated that an inflammatory response in the sympathetic trunk can be caused by anterior herniation of the lumbar disc,which can generate a sympathetic inflammatory pain response. | Yuanzhang Tang Jingjing Bian Yuna Guo Chenli Sun Yawei Xiuhua Li Jiaxiang Ni | 2017 | Translational Neuroscience and Clinics2017，3，4： | 0 |
| 12 | Mechanical Properties of Nano-CaCO_(3) and Basalt Fiber Reinforced Concrete:Experiments and Numerical Simulations显示文摘In this study,the compressive,split tensile,and flexural strengths of concrete with nano-CaCO_(3) only were compared with those of concrete with nano-CaCO_(3) and basalt fibers through field experiments,and the underlying mechanisms were analyzed by the Scanning Electron Microscope (SEM) techniques.On the mesoscale,a damage model of concrete was established based on the continuum progressive damage theory,which was used to investigate the influence of different lengths and contents of fibers on the mechanical properties of concrete.Then,the experimental and numerical simulation results were compared and analyzed to verify the feasibility of model.The results show that nano-CaCO_(3) can enhance the compressive strength of the concrete,with an optimal content of 2.0%.Adding basalt fibers into the nano-CaCO_(3) reinforced concrete may further enhance the compressive,split tensile,and flexural strengths of the concrete;however,the higher content of basalt fiber can not lead to higher performance of concrete.The optimal length and content of fiber are 6 mm and 0.20%,respectively.The SEM result shows that the aggregation of basalt fibers is detrimental to the mechanical properties of concrete.The numerical simulation results are in good agreement with the experimental results. | 刁目爽 任倩慧 SUN Xinjian ZHAO Yawei WEI Chengpeng | 2022 | Journal of Wuhan University of Technology(Materials Science)2022，37，5： | 0 |
| 13 | Enhanced CO2 electroreduction on armchair graphene nanoribbons edge-decorated with copper显示文摘由 graphene nanoribbons (GNR ) 和关于他们的边装饰的理论研究的最近的试验性的合成启发了,我们包括地为公司 2 学习了与 Cu 装饰边的 GNR 的 electrocatalytic 表演减小。比较了盘旋地行走 GNR ,有 2 是的 n = 3p +的宽度的 终止Cu 的扶手椅 GNR 为与不到 0.5 eV 的一个免费精力障碍从公司2生产甲醇的更有效的催化剂,比体积 Cu 和另外的 支持graphene 的 Cu 结构提供更低的过电位和更高的选择的优点。在另一方面,竞争的氢进化反应能被终止 Cu 的扶手椅 GNR 有效地压制。因此,装饰边的 GNR 为为公司 2 electroreduction 调节催化效率和选择提供大灵活性。 | Guizhi Zhu | 2017 | Nano Research2017，10，5： | 0 |
| 14 | THE EFFECTS OF TURBULENCE ON THE AERODYNAMIC PROPERTIES OF TWO-DIMENSIONAL RECTANGULAR PRISMS(Ⅱ)——ELASTICALLY SUPPORTED CASE显示文摘The effects of turbulence on the aerodynamic properties,especially surface-pressurespectra and vortex-induced vibrations,of two-dimensional rectangular prisms with slenderness ratiosof 2 and 4 are investigated experimentally.Particular attention is given to turbulence integral scale ef-fects from the view-point of energy distribution in frequency domain. | Sun Yawei (Department of Mechanics,Peking University) | 1989 | Acta Mechanica Sinica1989，5，2： | 0 |
| 15 | METTL14 is a chromatin regulator independent of its RNA N^(6)-methyladenosine methyltransferase activity显示文摘METTL3 and METTL14 are two components that form the core heterodimer of the main RNA m^(6)A methyltransferase complex(MTC)that installs m^(6)A.Surprisingly,depletion of METTL3 or METTL14 displayed distinct effects on stemness maintenance of mouse embryonic stem cell(mESC).While comparable global hypo-methylation in RNA m^(6)A was observed in Mettl3 or Mettl14 knockout mESCs,respectively.Mettl14 knockout led to a globally decreased nascent RNA synthesis,whereas Mettl3 depletion resulted in transcription upregulation,suggesting that METTL14 might possess an mA-independent role in gene regulation.We found that METTL14 colocalizes with the repressive H3K27me3 modification.Mechanistically,METTL14,but not METTL3,binds H3K27me3 and recruits KDM6B to induce H3K27me3 demethylation independent of METTL3.Depletion of METTL14 thus led to a global increase in H3K27me3 level along with a global gene suppression.The effects of METTL14 on regulation of H3K27me3 is essential for the transition from self-renewal to differentiation of mESCs.This work reveals a regulatory mechanism on heterochromatin by METTL14 in a manner distinct from METTL3 and independently of m^(6)A,and critically impacts transcriptional regulation,stemness maintenance,and differentiation ofmESCs. | Xiaoyang Dou Lulu Huang Yu Xiao Chang Liu Yini Li Xinning Zhang Lishan Yu Ran Zhao Lei Yang Chuan Chen Xianbin Yu Boyang Gao Meijie Qi Yawei Gao Bin Shen Shuying Sun Chuan He Jun Liu | 2023 | Protein & Cell2023，14，9： | 0 |
| 16 | ABE-induced PDGFD start codon silencing unveils new insights into the genetic architecture of sheep fat tails显示文摘Sheep is an important livestock species raised globally to produce meat,milk,wool,and other by-products.During the Neolithic Revolution,sheep were domesticated in the Fertile Crescent of Southwest Asia around 10,000 years ago(Chessa et al.,2009). | Peter Kalds Shuhong Huang Shiwei Zhou Siyuan Xi Yumeng Fang Yawei Gao Kexin Sun Chao Li Bei Cai Yao Liu Yige Ding Qifang Kou Tad Sonstegard Bjöm Petersen Stephen Kemp Baohua Ma Jian-Lin Han Yulin Chen Xiaolong Wang | 2023 | Journal of Genetics and Genomics2023，50，12： | 0 |
| 17 | Virus-like supramolecular assemblies formed by cooperation of base pairing interaction and peptidic association显示文摘A peptide nucleic acid(PNA)-peptide conjugated molecule, T′_3(AKAE)_2, was designed to have both a PNA segment for oligonucleotide binding and an ionic self-complementary peptide sequence for self-association. T′_3(AKAE)_2 could co-assemble with oligoadenines(d(A)_x) to form virus-like supramolecular structures whose morphology showed dependence on the chain length and rigidity of the d(A)_x molecules. Smaller nanospheres with diameters of 13.0±2.0 nm were produced in the case of d(A)_6. Wormlike aggregates with lengths of 20–50 nm and diameters of 15.0±2.5 nm were found in the cases of d(A)_(12), d(A)_(18), d(A)_(24) and d(A)_(30). And larger spherical aggregates with diameters of 18±5 nm came into presence in the cases of d(A)_(36) and d(A)_(42). These nanostructures were suggested to be formed under a cooperative effect of base pair recognition and peptidic association. The study provides insights into the programmed assembly of a multi-components system as well as control of the size and shape of the co-assembled structures, which is of great significance in developing gene/drug delivery systems. | Meiwen Cao Ningning Wang Peng Zhou Yawei Sun Jiqian Wang Shengjie Wang Hai Xu | 2016 | Science China Chemistry2016，59，3： | 0 |
| 18 | Research on the design of metalens with achromatic and amplitude modulation显示文摘Metalenses are two-dimensional planar metamaterial lenses, which have the advantages of high efficiency and easy integration. However, most metalenses cannot modulate the light intensity, which limits their applications. To deal with it, taking advantage of flexible regulation of the beam amplitude and phase by the metalens, the geometric phase method is selected to design the dual-function metalens. It can effectively eliminate chromatic aberration in a visible light band from 535 nm to 600 nm and achieve amplitude modulation. After transmitting the metalens, the amplitudes of the beam respectively turn into 0.2 and 0.9. In this way, the amount of transmission of metalens in the preset band can be quantitatively controlled. According to the distribution characteristics of light diffraction intensity, the metalens designed can play a dual modulation role of achromatism and interference double-beam equilibrium in the paper, to meet the needs of miniaturization and integration of the optical system. The achromatic and amplitude-modulated metalens will have great application potential in optical holographic imaging and super-resolution focusing. | XU Yuanyuan GENG Yan LIANG Yu TANG Furui SUN Yujuan WANG Yawei | 2023 | Optoelectronics Letters2023，19，2： | 0 |
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