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| 1 | Apoptosis during β-mercaptoethanol-induced differentiation of adult adipose-derived stromal cells into neurons显示文摘β-mercaptoethanol can induce adipose-derived stromal cells to rapidly and efficiently differentiate into neurons in vitro.However,because of the short survival time of the differentiated cells,clinical applications for this technique are limited.As such,we examined apoptosis of neurons differentiated from adipose-derived stromal cells induced with β-mercaptoethanol in vitro using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and transmission electron microscopy.The results revealed that the number of surviving cells decreased and apoptosis rate increased as induction time extended.Taken together,these results suggest that apoptosis occurring in the process of adipose-derived stromal cells differentiating into neurons is the main cause of cell death.However,the mechanism underlying cellular apoptosis should be researched further to develop methods of controlling apoptosis for clinical applications. | Yanan Cai Xiaodong Yuan Ya Ou Yanhui Lu | 2011 | Neural Regeneration Research2011，6，10： | 19 |
| 2 | Ultrastructure of neuronal-like cells differentiated from adult adipose-derived stromal cells显示文摘β-mercaptoethanol induces in vitro adult adipose-derived stromal cells(ADSCs) to differentiate into neurons.However,the ultrastructural features of the differentiated neuronal-like cells remain unknown.In the present study,inverted phase contrast microscopy was utilized to observe β-mercaptoethanol-induced differentiation of neuronal-like cells from human ADSCs,and immunocytochemistry and real-time polymerase chain reaction were employed to detect expression of a neural stem cells marker(nestin),a neuronal marker(neuron-specific enolase),and a glial marker(glial fibrillary acidic protein).In addition,ultrastructure of neuronal-like cells was observed by transmission election microscopy.Results revealed highest expression rate of nestin and neuron-specific enolase at 3 and 5 hours following induced differentiation;cells in the 5-hour induction group exhibited a neuronal-specific structure,i.e.,Nissl bodies.However,when induction solution was replaced by complete culture medium after 8-hour induction,the differentiated cells reverted to the fibroblast-like morphology from day 1.These results demonstrate that β-mercaptoethanol-induced ADSCs induced differentiation into neural stem cells,followed by morphology of neuronal-like cells.However,this differentiation state was not stable. | Changqing Ye Xiaodong Yuan Hui Liu Yanan Cai | 2010 | Neural Regeneration Research2010，5，19： | 16 |
| 3 | A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes显示文摘The quantity and survival time of astrocytes,which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine,have thus far been unsatisfactory.The present study investigated the growth and differentiation characteristics of induced astrocytes by observing their growth curves.After induction for 48 hours with an inducer containing 0.5% ethanol,some adult adult adipose-derived stromal cells displayed typical astrocytic morphology.The cell quantity gradually decreased with prolonged induction time.Nestin,glial fibrillary acidic protein,and S-100 expression reached peak levels at 14 days,but neuron-specific enolase was not expressed.These results suggest that the induced astrocytes reached their peak at 14 days.Further optimization of the culture environment may yield mature astrocytes with normal functions,in greater quantity,and prolonged survival time. | Ya Ou Xiaodong Yuan Yanan Cai Yanhui Lu | 2011 | Neural Regeneration Research2011，6，10： | 12 |
| 4 | High-speed image reconstruction for optically sectioned,super-resolution structured illumination microscopy显示文摘Super-resolution structured illumination microscopy(SR-SIM)is an outstanding method for visualizing the subcellular dynamics in living cells.To date,by using elaborately designed systems and algorithms,SR-SIM can achieve rapid,optically sectioned,SR observation with hundreds to thousands of time points.However,real-time observation is still out of reach for most SIM setups as conventional algorithms for image reconstruction involve a heavy computing burden.To address this limitation,an accelerated reconstruction algorithm was developed by implementing a simplified workflow for SR-SIM,termed joint space and frequency reconstruction.This algorithm results in an 80-fold improvement in reconstruction speed relative to the widely used Wiener-SIM.Critically,the increased processing speed does not come at the expense of spatial resolution or sectioning capability,as demonstrated by live imaging of microtubule dynamics and mitochondrial tubulation. | Zhaojun Wang Tianyu Zhao Huiwen Hao Yanan Cai Kun Feng Xue Yun Yansheng Liang Shaowei Wang Yujie Sun Piero RBianco Kwangsung Oh Ming Lei | 2022 | Advanced Photonics2022，4，2： | 9 |
| 5 | Nucleocytoplasmic Trafficking of the Arabidopsis WD40 Repeat Protein XIW1 Regulates ABI5 Stability and Abscisic Acid Responses显示文摘WD40 repeat-containing proteins(WD40 proteins)serve as versatile scaffolds for protein-protein interac-tions,modulating a variety of cellular processes such as plant stress and hormone responses.Here we report the identification of a WD40 protein,XIW1(for XPO1-interacting WD40 protein 1),which positively regulates the abscisic acid(ABA)response in Arabidopsis.XIW1 is located in the cytoplasm and nucleus.We found that it interacts with the nuclear transport receptor XPO1 and is exported by XPO1 from the nucleus.Mutation of XIW1 reduces the induction of ABA-responsive genes and the accumulation of ABA Insensitive 5(ABI5),causing mutant plants with ABA-insensitive phenotypes during seed germination and seedling growth,and decreased drought stress resistance.ABA treatment upregulates the expression of XIW1,and both ABA and abiotic stresses promote XIW1 accumulation in the nucleus,where it interacts with ABI5.Loss of XIW1 function results in rapid proteasomal degradation of ABI5.Taken together,these findings suggest that XIW1 is a nucleocytoplasmic shuttling protein and plays a positive role in ABA responses by interacting with and maintaining the stability of ABI5 in the nucleus. | Xuezhong XU Wang Wan Guobin Jiang Yue Xi Haijian Huang Jiajia Cai Yanan Chang Cheng-Guo Duan Satendra KMangrauthia Xinxiang Peng Jian-Kang Zhu Guohui Zhu | 2019 | Molecular Plant2019，12，12： | 8 |
| 6 | Adult adipose-derived stromal cells differentiate into neurons with normal electrophysiological functions显示文摘β-mercaptoethanol was used to induce in vitro neuronal differentiation of adipose-derived stromal cells. Within an 8-hour period post-differentiation, the induced cells exhibited typical neuronal morphology, and expression of microtubule-associated protein 2 and neuron-specific enolase, which are markers of mature neurons, reached a peak at 5 hours. Specific organelle Nissl bodies of neurons were observed under transmission electron microscopy. Results of membrane potential showed that fluorescence intensity of cells was greater after 5 hours than adipose-derived stromal cells prior to induction. In addition, following stimulation with high-concentration potassium solution, fluorescence intensity increased. These experimental findings suggested that neurons differentiated from adipose-derived stromal cells and expressed mature K+ channels. In addition, following stimulation with high potassium solution, the membrane potential depolarized and fired an action potential, confirming that the induced cells possessed electrophysiological functions. | Xiaodong Yuan Yanan Cai Ya Ou Yanhui Lu | 2011 | Neural Regeneration Research2011，6，34： | 8 |
| 7 | Recent progress of integrated circuits and optoelectronic chips显示文摘Integrated circuits(ICs)and optoelectronic chips are the foundation stones of the modern information society.The IC industry has been driven by the so-called'Moore's law'in the past 60 years,and now has entered the post Moore's law era.In this paper,we review the recent progress of ICs and optoelectronic chips.The research status,technical challenges and development trend of devices,chips and integrated technologies of typical IC and optoelectronic chips are focused on.The main contents include the development law of IC and optoelectronic chip technology,the IC design and processing technology,emerging memory and chip architecture,brain-like chip structure and its mechanism,heterogeneous integration,quantum chip technology,silicon photonics chip technology,integrated microwave photonic chip,and optoelectronic hybrid integrated chip. | Yue HAO Shuiying XIANG Genquan HAN Jincheng ZHANG Xiaohua MA Zhangming ZHU Xingxing GUO Yahui ZHANG Yanan HAN Ziwei SONG Yan LIU Ling YANG Hong ZHOU Jiangyi SHI Wei ZHANG Min XU Weisheng ZHAO Biao PAN Yangqi HUANG Qi LIU Yimao CAI Jian ZHU Xin OU Tiangui YOU Huaqiang WU Bin GAO Zhiyong ZHANG Guoping GUO Yonghua CHEN Yong LIU Xiangfei CHEN Chunlai XUE Xingjun WANG Lixia ZHAO Xihua ZOU Lianshan YAN Ming LI | 2021 | Science China(Information Sciences)2021，64，10： | 7 |
| 8 | Autophagy and apoptosis during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro显示文摘β-mercaptoethanol can induce adult adipose-derived stromal cells to rapidly and efficiently differentiate into typical neuron-like cells in vitro.Immunohistochemistry showed that neuron specific enolase and neurofilament-200 expression gradually increased with the extension of induction time,and peaked at 5 hours.By contrast,glial fibrillary acidic protein was negatively expressed at all time points.Induced cells possessed a typical Nissl body,apoptosis showing condensed chromatin in the nucleus,autophagosomes with a bilayered membrane and autolysosomes in the cytoplasm at 5 hours.TUNEL assay and immunohistochemistry and immunofluorescence demonstrated that apoptosis and caspase-3 expression increased and peaked at 8 hours.Immunohistochemistry and immunofluorescence showed that microtubule-associated protein light chain 3 gradually increased with induction and reached a peak at 5 hours.These results indicate that autophagy played an important role in protecting cells during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro. | Yanhui Lu Xiaodong Yuan Ya Ou Yanan Cai Shujuan Wang Qiaoyu Sun Wenli Zhang | 2012 | Neural Regeneration Research2012，7，16： | 5 |
| 9 | Removal of elemental mercury by photocatalytic oxidation over La_(2)O_(3)/Bi_(2)O_(3)composite显示文摘La_(2)O_(3)/Bi_(2)O_(3)photocatalysts were prepared by impregnation of Bi_(2)O_(3)with an aqueous solution of lanthanum precursor followed by calcination at different temperatures.The composite materials were used for the first time for the photocatalytic removal of Hg~0 from a simulated flue gas under UV light irradiation.The results showed that the sample containing 6 wt.%La_(2)O_(3)and calcined at 500°C has the highest dispersion of the active sites,which was promoted by the strong interaction with the support(i.e.,the formation of Bi-O-La species).Since they are fully accessible on the surface,the material also exhibits excellent optical properties while the heterojunction formed in La_(2)O_(3)/Bi_(2)O_(3)promotes the separation and migration of photoelectron-hole pairs and thus Hg~0 oxidation efficiency is enhanced.The effects of the various factors(e.g.,the reaction temperature and composition of the simulated flue gas(i.e.,O_(2),NO,H_(2)O,and SO_(2)))on the efficiency of the Hg~0 photocatalytic oxidation were investigated.The results demonstrated that O_(2)and SO_(2)enhanced the efficiency of the reaction while the reaction temperature,NO,and H_(2)O had an inhibitory effect. | Renjun Li Yanan Zhou Xueqian Wang Langlang Wang Ping Ning Lei Tao Jun Cai | 2021 | Journal of Environmental Sciences2021，33，4： | 2 |
| 10 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 11 | Low levels of neutralizing antibodies against XBB Omicron subvariants after BA.5 infection显示文摘The COVID-19 response strategies in Chinese mainland were recently adjusted due to the reduced pathogenicity and enhanced infectivity of Omicron subvariants.In Chengdu,China,an infection wave was predominantly induced by the BA.5 subvariant.It is crucial to determine whether the hybrid anti-SARS-CoV-2 immunity following BA.5 infection. | Jingyun Yang Weiqi Hong Hong Lei Cai He Wenwen Lei Yanan Zhou Tingmei Zhao Aqu Alu Xuelei Ma Jiong Li Li Yang Zhenling Wang Wei Wang Guangwen Lu Guobo Shen Shuaiyao Lu Guizhen Wu Huashan Shi Xiawei Wei | 2023 | Signal Transduction and Targeted Therapy2023，8，7： | 1 |
| 12 | Tablets of paliperidone using compression-coated technology for controlled ascending release显示文摘The aim of this work was to prepare ascending release compression-coated(CC) tablets with paliperidone(PAL) using a simple manufacturing technique and short manufacturing process.The release behavior and mechanisms in vitro of the final tablets was investigated and evaluated. The PAL CC tablets were comprised of a core layer of high viscosity hydroxypropyl cellulose(HPC-H) and a coating layer of high viscosity hydroxypropyl methylcellulose(HPMCK100 M). Several factors such as materials and core tablet compositions were studied for their influence in the formulation procedure. The drug release mechanism was studied using gravimetric analysis. The data could be fitted to the Peppas model. The ascending drug release results were expressed in terms of the slope of the release curve at different time points.Results showed that the formulation could achieve a good ascending drug release when the weight ratio of PAL was 5:1(core:layer). The fraction of HPC and HPMC was 33 %, and the combination of Eudragit RL-PO was 10%. The ascending release mechanism was due to solvent penetration into the PAL CC tablets, and subsequent drug dissolution from the gelatinous HPC and HPMC matrix erosion. The release mechanism was therefore a combination of diffusion and erosion. This work demonstrated that the compression-coated tablets could achieve controlled ascending release over 24 h for the oral administration systems. | Yingying Tang Huan Teng Yanan Shi Haibing He Yu Zhang Tian Yin Cuifang Cai Xing Tang | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，2： | 1 |
| 13 | Career adaptability, job search self-efficacy and outcomes: A three-wave investigation among Chinese university graduates显示文摘 | Yanjun Guan Hong Deng Jiaqing Sun Yanan Wang Zijun Cai Lihui Ye Ruchunyi Fu Yang Wang Shu Zhang Yuhui Li | 2013 | Journal of Vocational Behavior2013，，3： | 1 |
| 14 | Multi- objective community detection in complex networks 显示文摘 | SHI Chuan YAN Zhenyu CAI Yanan | 2012 | Applied Soft Computing2012，12，2： | 1 |
| 15 | Simple Harish-Chandra supermodules over the super Schrdinger algebra显示文摘We study the N=1 super Schrdinger algebra S in(1+1)-dimensional spacetime. The first part of this paper determines the necessary and sufficient conditions for highest weight supermodules over S to be simple. The paper also describes the structure of all Verma supermodules and determines all simple Harish-Chandra supermodules over S. | CAI YanAn GAO Yun WANG YongJie | 2015 | Science China Mathematics2015，58，12： | 1 |
| 16 | p 显示文摘 | Yang Yanan Cai Jing Zhuang Xiuli | 2010 | Polymer2010，51，12： | 1 |
| 17 | Effective start-up biofiltration method for Fe, Mn, and ammonia removal and bacterial community analysis显示文摘 | CAI Yanan LI Dong LIANG Yuwen | 2015 | Bioresource Technology2015，176，： | 1 |
| 18 | Selective leaching of silver-rich residues in NH4SCN solu- tion under oxygen pressure显示文摘 | Shenghai Yang Gengtao Chen Yanan Cai | 2011 | Separation and Purification Technology2011，77，1： | 1 |
| 19 | pH-dependent self-assembly of amphiphilic poly( l -glutamic acid)-block-poly(lactic-co-glycolic acid) copolymers显示文摘 | Yanan Yang Jing Cai Xiuli Zhuang Zhaopei Guo Xiabin Jing Xuesi Chen | 2010 | Polymer2010，，12： | 1 |
| 20 | Single-cell analysis reveals the origins and intrahepatic development of liver-resident IFN-γ-producing γδ T cells显示文摘γδ T cells are heterogeneous lymphocytes located in various tissues.However,a systematic and comprehensive understanding of the origins of γδ T cell heterogeneity and the extrathymic developmental pathway associated with liver γδ T cells remain largely unsolved.In this study,we performed single-cell RNA sequencing(scRNA-seq)to comprehensively catalog the heterogeneity of γδ T cells derived from murine liver and thymus samples.We revealed the developmental trajectory of γδ T cells and found that the liver contains γδ T cell precursors(pre-γδ T cells).The developmental potential of hepatic γδ T precursor cells was confirmed through in vitro coculture experiments and in vivo adoptive transfer experiments.The adoptive transfer of hematopoietic progenitor Lin^(-)Sca-1^(+)Mac-1^(+)(LSM)cells from fetal or adult liver samples to sublethally irradiated recipients resulted in the differentiation of liver LSM cells into pre-γδ T cells and interferon-gamma^(+)(IFN-γ^(+))but not interleukin-17a^(+)(IL-17a^(+))γδ T cells in the liver.Importantly,thymectomized mouse models showed that IFN-γ-producing γδ T cells could originate from liver LSM cells in a thymus-independent manner.These results suggested that liver hematopoietic progenitor LSM cells were able to differentiate into pre-γδ T cells and functionally mature γδ T cells,which implied that these cells are involved in a distinct developmental pathway independent of thymus-derived γδ T cells. | Yuan Hu Keke Fang Yanan Wang Nan Lu Haoyu Sun Cai Zhang | 2021 | Cellular & Molecular Immunology2021，18，4： | 1 |
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