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| 1 | Confined Ni-In intermetallic alloy nanocatalyst with excellent coking resistance for methane dry reforming显示文摘Carbon dioxide and methane are two main greenhouse gases which are contributed to serious global warming.Fortunately,dry reforming of methane(DRM),a very important reaction developed decades ago,can convert these two major greenhouse gases into value-added syngas or hydrogen.The main problem retarding its industrialization is the seriously coking formation upon the nickel-based catalysts.Herein,a series of confined indium-nickel(In-Ni)intermetallic alloy nanocatalysts(In_(x)Ni@SiO_(2))have been prepared and displayed superior coking resistance for DRM reaction.The sample containing 0.5 wt.%of In loading(In_(0.5)Ni@SiO_(2))shows the best balance of carbon deposition resistance and DRM reactivity even after 430 h long term stability test.The boosted carbon resistance can be ascribed to the confinement of core–shell structure and to the transfer of electrons from Indium to Nickel in In-Ni intermetallic alloys due to the smaller electronegativity of In.Both the silica shell and the increase of electron cloud density on metallic Ni can weaken the ability of Ni to activate C–H bond and decrease the deep cracking process of methane.The reaction over the confined InNi intermetallic alloy nanocatalyst was conformed to the Langmuir-Hinshelwood(L-H)mechanism revealed by in situ diffuse reflectance infrared Fourier transform spectroscopy(in-situ DRIFTS).This work provides a guidance to design high performance coking resistance catalysts for methane dry reforming to efficiently utilize these two main greenhouse gases. | Wenming Liu Le Li Sixue Lin Yiwei Luo Zhenghong Bao Yiru Mao Kongzhai Li Daishe Wu Honggen Peng | 2022 | Journal of Energy Chemistry2022，31，2： | 3 |
| 2 | Fe3O4 Octahedral Colloidal Crystals显示文摘我们在场为没有底层的帮助的高度命令的八面的 Fe3O4 胶体的单个晶体的大规模制造的一个灵巧、可控制的方法。油的酸被用来在胶体的答案减少 nano 大楼块的溶解度并且导致一个结晶化过程。我们的胶体的晶体具有 multimicron 尺寸并且显示出典型结晶的特征。他们有很柔韧的结构并且当一篇小说与相对狭窄的毛孔尺寸分布订了磁性的 mesoporous 材料,能服务。样品基于它的好 crystallinity,以及 interparticle 在 5 K 拥有 100 K 的极其高的 Verwey 转变温度(T V ) 和 86 emu/g 的高浸透磁化(M S ) 偶极的相互作用行为从它的唯一的结构产生。电气化学的大小表明了 mesoporous 的优秀能力胶体的晶体当在锂离子电池使用了时。 | L-r Meng Weimeng Chen Yiwei Tan Lin Zou Chinping Chen Heping Zhou Qing Peng Yadong Li | 2011 | Nano Research2011，4，4： | 3 |
| 3 | Breakthrough Technologies for the Biorefining of Organic Solid and Liquid Wastes显示文摘有机固液废弃物包含大量能量、养分和水分,不应仅视其为废物。数十年来,人们一直对城市固体废物进行回收、堆制和燃烧,并从中提取能量和有价值的物质。污水的首要管理策略是治理和排放。随着技术的进步,通过新方法使利用固体废物和废水成为可能。考虑到废物特殊的化学、物理和生物性质,需要整合多种技术以使废物的能量和价值回收最大化。为此,生物炼制是完全利用废物中的能量和价值的一个合适的方法。研究证明,不可回收废弃物和生物固体可通过不同方法转化为可利用的热能、电能、燃料和化学品,并且液体废物或能帮助农作物和藻类的成长,为能量回收和食物生产提供不同的选择。本文针对有机固液废物提出新型生物炼制方案,这些废物来源于城市废料、食品和生物加工厂以及动物生产设施。四大新型突破性科技——真空辅助高温厌氧消化、扩展鱼菜共生系统、含油废物经甘油裂解制取生物柴油和微波辅助热化学转化,均可运用于生物炼制方案中,从而成功将废物转化,用以生产化学品、化肥、能量(沼气、合成气、生物柴油和生物油)、食物、饲料,得到干净的水并显著减少污染物的排放。 | Paul Chen Erik Anderson Min Addy Renchuan Zhang Yanling Cheng Peng Peng Yiwei Ma Liangliang Fan Yaning Zhang Qian Lu Shiyu Liu Nan Zhou Xiangyuan Deng Wenguang Zhou Muhammad Omar Richard Griffith Faryal Kabir Hanwu Lei Yunpu Wang Yuhuan Liu Roger Ruan | 2018 | Engineering2018，4，4： | 3 |
| 4 | Crystal structure of histidine-containing phosphocarrier protein from Thermoanaerobacter tengcongensis MB4 and the implications for thermostability显示文摘Protein thermostability is an inherent characteristic of proteins from thermophilic microorganisms,and therefore enables these organisms to survive at extreme temperatures.Although it is well-known that thermostable proteins are critical for the growth of thermophilic organisms,the structural basis of protein thermostability is not yet fully understood.The histidine-containing phosphocarrier (HPr) protein,a phosphate shuttle protein in the phosphoenolpyruvate-dependent sugar transport system (PTS) of bacterial species,is an ideal model for investigating protein thermostability with respect to its small size and deficiency in disulphide bonds or cofactors.In this study,the HPr protein from Thermoanaerobacter tengcongensis (TtHPr) is cloned and purified.Crystal structure with good quality has been determined at 2.3 resolution,which provides a firm foundation for exploring the thermostable mechanism.However,it shows that the crystal structure is conserved and no clue can be obtained from this single structure.Furthermore,detailed comparison of sequence and structure with the homologs from mesoor thermophilic bacteria shows no obvious rule for thermostability,but the extra salt-bridge existing only in thermophilic bacteria might be a better explanation for thermostability of HPr.Thus,mutations are performed to interrupt the salt-bridge in HPrs in thermophilic bacteria.Using site-directed mutations and the circular dichroism method,thermostability is evaluated,and the mutational variations are shown to have a faster denaturing rate than for wild-type viruses,indicating that mutations cause instability in the HPrs.Understanding the higher-temperature resistance of thermophilic and hyperthermophilic proteins is essential to studies on protein folding and stability,and is critical in engineering efficient enzymes that can work at a high temperature. | FENG ChunYan GAO Feng LIU YiWei WANG GuiHua PENG Hao MA YanHe YAN JingHua GAO George Fu | 2011 | Science China(Life Sciences)2011，54，6： | 2 |
| 5 | Controllable fabrication and electrical performance of single crystalline Cu2 O nanowires with high aspect ratios显示文摘 | Tan Yiwei Xue Xinyu Peng Qing | 2007 | Nano Lett2007，7，12： | 1 |
| 6 | The C_(6)D_(6)detector system on the Back-n beam line of CSNS显示文摘Introduction The neutron capture cross sections are very important in the field of nuclear device design and basic physics research.Hydrogen-free liquid scintillator such as C_(6)D_(6)detectors are widely used in the neutron capture cross-sectional measurements for the low neutron sensitivity and fast time response.The Back-n white neutron source at China Spallation Neutron Source is the first spallation white neutron source in China,and it is suitable for neutron capture cross-sectional measurement.Materials and methods A C_(6)D_(6)detector system was built in the Back-n experimental station.The pulse height weighting technique was used to determine the system’s detection efficiency.The response to gamma rays of the C_(6)D_(6)detector was measured,and the energy resolution function was determined.Monte Carlo simulation with Geant4 code was carried out to get the weighting function of this C_(6)D_(6)detector system.Additionally,the systematic uncertainty of the weighting function was also determined.Conclusion According to the experimental and simulation results,this C_(6)D_(6)detector system can be used to measure neutron capture cross section. | Jie Ren Xichao Ruan Jie Bao Guangyuan Luan Wei Jiang Qi An Huaiyong Bai Ping Cao Qiping Chen Yonghao Chen Pinjing Cheng Zengqi Cui Ruirui Fan Changqing Feng Minhao Gu Fengqin Guo Changcai Han Zijie Han Guozhu He Yongcheng He Yuefeng He Hanxiong Huang Weiling Huang Xiru Huang Xiaolu Ji Xuyang Ji Haoyu Jiang Hantao Jing Ling Kang Mingtao Kang Bo Li Lun Li Qiang Li Xiao Li Yang Li Yang Li Rong Liu Shubin Liu Xingyan Liu Yinglin Ma Changjun Ning Binbin Qi Zhaohui Song Hong Sun Xiaoyang Sun Zhijia Sun Zhixin Tan Hongqing Tang Jingyu Tang Pengcheng Wang Qi Wang Taofeng Wang Yanfeng Wang Zhaohui Wang Zheng Wang Jie Wen Zhongwei Wen Qingbiao Wu Xiaoguang Wu Xuan Wu Likun Xie Yiwei Yang Han Yi Li Yu Tao Yu Yongji Yu Guohui Zhang Jing Zhang Linhao Zhang Liying Zhang Qingmin Zhang Qiwei Zhang Xianpeng Zhang Yuliang Zhang Zhiyong Zhang Yingtan Zhao Liang Zhou Zuying Zhou Danyang Zhu Kejun Zhu Peng Zhu | 2019 | Radiation Detection Technology and Methods2019，3，3： | 1 |
| 7 | One-Dimensional Single-Crystalline Mn3O4Nanostructures with Tunable Length and Magnetic Properties of Mn3O4Nanowires显示文摘 | Tan Yiwei Meng Lingrong Peng Qing | 2011 | Chemical Communications2011，47，4： | 1 |
| 8 | Temperature dependence of the ionization coefficients of InAlAs and AlGaAs digital alloys显示文摘Digital alloy In_(0.52)Al_(0.48) As avalanche photodiodes exhibit lower excess noise than those fabricated from random alloys. This paper compares the temperature dependence, from 203 to 323 K, of the impact ionization characteristics of In_(0.52)Al_(0.48) As and Al_(0.74)Ga_(0.26) As digital and random alloys. These results provide insight into the low excess noise exhibited by some digital alloy materials, and these materials can even obtain lower excess noise at low temperature. | YUAN YUAN JIYUAN ZHENG YAOHUA TAN YIWEI PENG ANN-KATHRYN ROCKWELL SETH R. BANK AVIK GHOSH JOE C. CAMPBELL | 2018 | Photonics Research2018，6，8： | 1 |
| 9 | Towards the Deep Convergence of Communication and Computing in RAN: Scenarios, Architecture, Key Technologies, Challenges and Future Trends显示文摘To accommodate the diversified emerging use cases in 5G,radio access networks(RAN)is required to be more flexible,open,and versatile.It is evolving towards cloudification,intelligence and openness.By embedding computing capabilities within RAN,it helps to transform RAN into a natural cost effective radio edge computing platform,offering great opportunity to further enhance RAN agility for diversified services and improve users’quality of experience(Qo E).In this article,a logical architecture enabling deep convergence of communication and computing in RAN is proposed based on O-RAN.The scenarios and potential benefits of sharing RAN computing resources are first analyzed.Then,the requirements,design principles and logical architecture are introduced.Involved key technologies are also discussed,including heterogeneous computing infrastructure,unified computing and communication task modeling,joint communication and computing orchestration and RAN computing data routing.Followed by that,a VR use case is studied to illustrate the superiority of the joint communication and computing optimization.Finally,challenges and future trends are highlighted to provide some insights on the potential future work for researchers in this field. | Nan Li Qi Sun Xiang Li Fengxian Guo Yuhong Huang Ziqi Chen Yiwei Yan Mugen Peng | 2023 | China Communications2023，20，3： | 1 |
| 10 | On End-to-End Architecture for Transporting MPEG-4 Video over the Internet 显示文摘 | Da peng Wu Yiwei Thomas Hou Wenwu Zhu etal | 2000 | IEEE Trans on Circuits and Systems for Video Technology2000，10，6： | 1 |
| 11 | The miR-27a-3p/FTO axis modifies hypoxia-induced malignant behaviors of glioma cells显示文摘Glioblastoma multiforme(GBM)is one of the most malignant types of central nervous system(CNS)tumors.N6-methyladenine(m6A)RNA modification is a main type of RNA modification in eukaryotic cells.In this study,we find that the m6A RNA methylation eraser FTO is dramatically downregulated in glioma samples and cell lines,particularly in intermediate and core regions and hypoxia-challenged glioma cells.In vitro,FTO overexpression inhibits the hypoxia-induced capacities of glioma cells to proliferate,migrate and invade,and decreases the percentage of cells with m6A RNA methylation.In vivo,FTO overexpression inhibits tumor growth in the xenograft model and decreases the protein levels of migration markers,including Vimentin and Twist.miR-27a-3p is upregulated within glioma intermediate and core regions and hypoxia-challenged glioma cells.miR-27a-3p inhibits the expression of FTO via direct binding to FTO.miR-27a-3p overexpression promotes hypoxia-challenged glioma cell aggressiveness,whereas FTO overexpression partially diminishes the oncogenic effects of miR-27a-3p overexpression.FTO overexpression promotes the nuclear translocation of FOXO3a and upregulates the expression levels of the FOXO3a downstream targets BIM,BNIP3,BCL-6,and PUMA,possibly by interacting with FOXO3a.Conclusively,FTO serves as a tumor suppressor in glioma by suppressing hypoxia-induced malignant behaviors of glioma cells,possibly by promoting the nuclear translocation of FOXO3a and upregulating FOXO3a downstream targets.miR-27a-3p is a major contributor to FTO downregulation in glioma under hypoxia. | Peng Du Li Meng Xinbin Liao Yi Liu Xin Mo Mengqi Gong Yiwei Liao | 2023 | Acta Biochimica et Biophysica Sinica2023，55，1： | 1 |
| 12 | Laparoscopic Low Anterior Resection and Eversion Technique Combined With a Nondog Ear Anastomosis for Mid- and Distal Rectal Neoplasms: A Preliminary and Feasibility Study显示文摘 | Changhua Zhuo Lei Liang Mingang Ying Qingguo Li Dawei Li Yiwei Li Junjie Peng Liyong Huang Sanjun Cai Xinxiang Li | 2015 | Medicine2015，，50： | 1 |
| 13 | Contact engineering for two-dimensional semiconductors显示文摘Two-dimensional(2D)layered materials,including graphene,black phosphorus(BP)and transition metal dichalcogenide(TMD)such as molybdenum disulfide(Mo S2),tungsten diselenide(WSe2),have attracted increasing attention for the application in electronic and optoelectronic devices.Contacts,which are the communication links between these 2D materials and external circuitry,have significant effects on the performance of electronic and optoelectronic devices.However,the performance of devices based on 2D semiconductors(SCs)is often limited by the contacts.Here,we provide a comprehensive overview of the basic physics and role of contacts in 2D SCs,elucidating Schottky barrier nature and Fermi level pinning effect at metal/2D SCs contact interface.The progress of contact engineering,including traditional metals contacts and metallic 2D materials contacts,for improving the performance of 2D SCs based devices is presented.Traditional metal contacts,named 3D top and edge contacts,are discussed briefly.Meanwhile,methods of building 2D materials contacts(2D top contact and 2D edge contact)are discussed in detail,such as chemical vapor deposition(CVD)growth of 2D metallic material contacts,phase engineered metallic phase contacts and intercalation induced metallic state contacts.Finally,the challenges and opportunities of contact engineering for 2D SCs are outlined. | Peng Zhang Yiwei Zhang Yi Wei Huaning Jiang Xingguo Wang Yongji Gong | 2020 | Journal of Semiconductors2020，41，7： | 0 |
| 14 | Novel Quantum Algorithms to Minimize Switching Functions Based on Graph Parti显示文摘After Google reported its realization of quantum supremacy,Solving the classical problems with quantum computing is becoming a valuable research topic.Switching function minimization is an important problem in Electronic Design Automation(EDA)and logic synthesis,most of the solutions are based on heuristic algorithms with a classical computer,it is a good practice to solve this problem with a quantum processer.In this paper,we introduce a new hybrid classic quantum algorithm using Grover’s algorithm and symmetric functions to minimize small Disjoint Sum of Product(DSOP)and Sum of Product(SOP)for Boolean switching functions.Our method is based on graph partitions for arbitrary graphs to regular graphs,which can be solved by a Grover-based quantum searching algorithm we proposed.The Oracle for this quantum algorithm is built from Boolean symmetric functions and implemented with Lattice diagrams.It is shown analytically and verified by simulations on a quantum simulator that our methods can find all solutions to these problems. | Peng Gao Marek Perkowski Yiwei Li Xiaoyu Song | 2022 | Computers, Materials & Continua2022，，3： | 0 |
| 15 | Genome degradation promotes Salmonella pathoadaptation by remodeling fimbriae-mediated proinflammatory response显示文摘Understanding changes in pathogen behavior(e.g.increased virulence,a shift in transmission channel)is critical for the public health management of emerging infectious diseases.Genome degradation via gene depletion or inactivation is recognized as a pathoadaptive feature of the pathogen evolving with the host.However,little is known about the exact role of genome degradation in affecting pathogenic behavior,and the underlying molecular detail has yet to be examined.Using large-scale global avian-restricted Salmonella genomes spanning more than a century,we projected the genetic diversity of Salmonella Pullorum(bvSP)by showing increasingly antimicrobial-resistant ST92 prevalent in Chinese flocks.The phylogenomic analysis identified three lineages in bvSP,with an enhancement of virulence in the two recently emerged lineages(L2/L3),as evidenced in chicken and embryo infection assays.Notably,the ancestor L1 lineage resembles the Salmonella serovars with higher metabolic flexibilities and more robust environmental tolerance,indicating stepwise evolutionary trajectories towards avian-restricted lineages.Pan-genome analysis pinpointed fimbrial degradation from a virulent lineage.The later engineeredfim-deletion mutant,and all other five fimbrial systems,revealed behavior switching that restricted horizontal fecal-oral transmission but boosted virulence in chicks.By depleting fimbrial appendages,bvSP established persistent replication with less proinflammation in chick macrophages and adopted vertical transovarial transmission,accompanied by ever-increasing intensification in the poultry industry.Together,we uncovered a previously unseen paradigm for remodeling bacterial surface appendages that supplements virulence-enhanced evolution with increased vertical transmission. | Xiao Zhou Xiamei Kang Jiaqi Chen Yan Song Chenghao Jia Lin Teng Yanting Tang Zhijie Jiang Xianqi Peng Xiaoxi Tao Yiwei Xu Linlin Huang Xuebin Xu Yaohui Xu Tengfei Zhang Shenye Yu Jiansen Gong Shaohui Wang Yuqing Liu Guoqiang Zhu Corinna Kehrenberg Franois-Xavier Weill Paul Barrow Yan Li Guoping Zhao Min Yue | 2023 | National Science Review2023，10，10： | 0 |
| 16 | Dihydroartemisinin increased the abundance of Akkermansia muciniphila by YAP1 depression that sensitizes hepatocellular carcinoma to anti-PD-1 immunotherapy显示文摘The effect of anti-programmed cell death 1(anti-PD-1)immunotherapy is limited in patients with hepatocellular carcinoma(HCC).Yes-associated protein 1(YAP1)expression increased in liver tumor cells in early HCC,and Akkermansia muciniphila abundance decreased in the colon.The response to anti-PD-1 treatment is associated with A.muciniphila abundance in many tumors.However,the interaction between A.muciniphila abundance and YAP1 expression remains unclear in HCC.Here,anti-PD-1 treatment decreased A.muciniphila abundance in the colon,but increased YAP1 expression in the tumor cells by mice with liver tumors in situ.Mechanistically,hepatocyte-specific Yap1 knockout(Yap1^(LKO))maintained bile acid homeostasis in the liver,resulting in an increased abundance of A.muciniphila in the colon.Yap1 knockout enhanced anti-PD-1 efficacy.Therefore,YAP1 inhibition is a potential target for increasing A.muciniphila abundance to promote anti-PD-1 efficacy in liver tumors.Dihydroartemisinin(DHA),acting as YAP1 inhibitor,increased A.muciniphila abundance to sensitize anti-PD-1 therapy.A.muciniphila by gavage increased the number and activation of CD8^(+)T cells in liver tumor niches during DHA treatment or combination with anti-PD-1.Our findings suggested that the combination anti-PD-1 with DHA is an effective strategy for liver tumor treatment. | Zhiqin Zhang Xinli Shi Jingmin Ji Yinglin Guo Qing Peng Liyuan Hao Yu Xue Yiwei Liu Caige Li Junlan Lu Kun Yu | 2023 | Frontiers of Medicine2023，17，4： | 0 |
| 17 | Bionic immunoactivator copresenting autophagy promoting and costimulatory molecules for synergistic cancer immunotherapy显示文摘Immunotherapy has great promise in improving malignant tumor treatment.However,the efficacy of existing strategies is often limited by the immunosuppressive environment.Here,we demonstrate an in situ bionic immunoactivator,PLT-Bec1/DTA-1,with possessed natural advantages of platelets for tumor recruitment and activation,on which DTA-1(CD357 monoclonal antibody)and Bec1 were tethered as combined immune boosters.PLT-Bec1/DTA-1,as a self-triggered release repository,can deliver the pre-tethered Bec1 and DTA-1 deeply through the secretion of platelet microparticles(PMPs),thereby cooperate tacitly and exhibit superiority in immune activation of dendritic cells(DCs)and T cells via autophagy inducibility,coupled with glucocorticoid-induced tumor necrosis factor receptor(GITR)-triggered T_(Reg) suppression,remodeled the immunosuppressive network of tumor microenvironment.PLT-Bec1/DTA-1 promoted antigen presentation and T cell proliferation,and alleviated the low activity state of bone marrow-derived dendritic cells(BMDCs)in tumor suppressive environment.PLT-Bec1/DTA-1 inhibited tumor recurrence(5-and 13-fold lower of control group in tumor volume)and CD8^(+)T/T_(Reg) ratio(6.3-and 8.8-fold vs.control group)in mouse tumor model after intravenous or subcutaneous administration.Also,PLT-Bec1/DTA-1 prevented tumor colonization in lung through in situ immune activation,and was slightly superior to the combined of Bec1 and PD-L1.Our findings highlight the promise of delivering immunostimulatory payloads via bionic carriers,eliciting automatic in situ activation of effector immune cells in tumor microenvironment for tumor eradication.All these results provide promising prospects into the application of immunoactivator in improving cancer synergistic immunotherapy to overcome the bottlenecks in clinic. | Yiwei Peng Yiliang Yang Zhenzhen Yang Datong Gao Jiajia Li Qi Sun Yitian Du Meng Lin Xianrong Qi | 2024 | Nano Research2024，17，3： | 0 |
| 18 | Superresolution structured illumination microscopy reconstruction algorithms:a review显示文摘Structured illumination microscopy(SIM)has become the standard for next-generation wide-field microscopy,offering ultrahigh imaging speed,superresolution,a large fiield-of-view,and long-term imaging.Over the past decade,SIM hardware and software have flourished,leading to successful applications in various biological questions.However,unlocking the full potential of SIM system hardware requires the development of advanced reconstruction algorithms.Here,we introduce the basic theory of two SIM algorithms,namely,optical sectioning SIM(OS-SIM)and superresolution SIM(SR-SIM),and summarize their implementation modalities.We then provide a brief overview of existing OS-SIM processing algorithms and review the development of SR-SIM reconstruction algorithms,focusing primarily on 2D-SIM,3D-SIM,and blind-SIM.To showcase the state-of-the-art development of SIM systems and assist users in selecting a commercial SIM system for a specific application,we compare the features of representative off-the-shelf SIM systems.Finally,we provide perspectives on the potential future developments of SIM. | Xin Chen Suyi Zhong Yiwei Hou Ruijie Cao Wenyi Wang Dong Li Qionghai Dai Donghyun Kim Peng Xi | 2023 | Light(Science & Applications)2023，12，8： | 0 |
| 19 | H_(2)S-assisted growth of 2D MS_(2)(M=Ti,Zr,Nb)显示文摘2D transition metal dichalcogenides (TMDCs) have drawn an enormous amount of attention due to their fascinating properties and application potential in next-generation information process and storage. However, the lack of proper synthesis approach limits their application. Here, we report a controllable synthesis method to grow ultrathin MS2 (M = Ti, Nb, Zr) nanosheets with H_(2)S-assisted chemical vapor deposition (CVD). We found that the presence of H_(2)S plays an important role to control the morphology of nanosheets including the lateral size and the nucleation density. With the assistance of H_(2)S, the growth of MS2 shows much thinner thickness with largely decreased nucleation density, beneficial for the device application, which can be attributed to the kinetics dominated growth. Our method hence opens a new avenue for the CVD growth of 2D TMDCs and the corresponding heterojunction, and paves the way for exploring their intriguing properties and applications. | Yiwei Zhang Peng Zhang Tengfei Xu Xingguo Wang Huaning Jiang Yongji Gong | 2022 | Chinese Chemical Letters2022，33，3： | 0 |
| 20 | A time-coded multi-concentration microfluidic chemical waveform generator for high-throughput probing suspension single-cell signaling显示文摘The cellular response to the complex extracellular microenvironment is highly dynamic in time and type of extracellular matrix.Accurately reconstructing this process and analyzing the changes in receptor conformation on the cell membrane surface and intracellular or intercellular signaling has been a major challenge in analytical chemistry and biophysical methodology.In this paper,a time-coded multiconcentration microfluidic chemical waveform generator was developed for the dynamic signaling probing with single-cell array of high temporal resolution,high throughput,and multi-concentration combination stimulation.Based on innovative microchannel structure,sophisticated external control methods and multiplexing technology,the system not only allowed for temporally sequential permutations of the four concentrations of stimuli(time code),but also generated pulsed and continuous waveforms at different frequencies in a highly controllable manner.Furthermore,the single-cell trap array was set up to efficiently capture cells in suspension,dramatically increasing throughput and reducing experiment preparation time.The maximum frequency of the platform was 1 Hz,and one cell could be stimulated at multiple frequencies.To show the ability of the system to investigate rapid biochemical events in high throughput,pulse stimulation and continuous stimulation of different frequencies and different time codes,combined with four concentrations of histamine(HA),were generated for probing G protein-coupled receptor(GPCR)signaling in He La cells.Then,statistical analysis was performed for the mean peak height and mean peak area of the cellular response.We believe that the time-coded multi-concentration microfluidic chemical waveform generator will provide a novel strategy for analytical chemistry,biophysics,cell signaling,and individualized medicine applications. | Yiran Guo Peng Chen Zhaolong Gao Yiwei Li Shunji Li Xiaojun Feng Bi-Feng Liu | 2022 | Chinese Chemical Letters2022，33，6： | 0 |
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