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| 1 | Durability of neutralizing antibodies and T-cell response post SARS-CoV-2 infection显示文摘The ongoing pandemic of coronavirus disease 19(COVID-19)is caused by a newly discoveredβcoronavirus named severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).How long the adaptive immunity triggered by SARS-CoV-2 can last is of critical clinical relevance in assessing the probability of second infection and efficacy of vaccination.Here we examined,using ELISA,the IgG antibodies in serum specimens collected from 17 COVID-19 patients at 6–7 months after diagnosis and the results were compared to those from cases investigated 2 weeks to 2 months post-infection.All samples were positive for IgGs against the S-and N-proteins of SARS-CoV-2.Notably,14 samples available at 6–7 months post-infection all showed significant neutralizing activities in a pseudovirus assay,with no difference in blocking the cell-entry of the 614D and 614G variants of SARS-CoV-2.Furthermore,in 10 blood samples from cases at 6–7 months post-infection used for memory T-cell tests,we found that interferonγ-producing CD4+and CD8+cells were increased upon SARS-CoV-2 antigen stimulation.Together,these results indicate that durable anti-SARS-CoV-2 immunity is common in convalescent population,and vaccines developed from 614D variant may offer protection from the currently predominant 614D variant of SARS-CoV-2. | Yun Tan Feng Liu Xiaoguang Xu Yun Ling Weijin Huang Zhaoqin Zhu Mingquan Guo Yixiao Lin Ziyu Fu Dongguo Liang Tengfei Zhang Jian Fan Miao Xu Hongzhou Lu Saijuan Chen | 2020 | Frontiers of Medicine2020，14，6： | 6 |
| 2 | Conversion of hepatoma cells to hepatocyte-like cells by defined hepatocyte nuclear factors显示文摘Normal cells become cancer cells after a malignant transformation,but whether cancer cells can be reversed to normal status remains elusive.Here,we report that the combination of hepatocyte nuclear factor IA (HNFIA),HNF4A and forkhead box protein A3 (FOXA3)synergistically reprograms hepatocellular carcinoma (HCC)cells to hepatocyte-like cells (reprogrammed hepatocytes, rHeps).Our results show that rHeps lose the malignant phenotypes of cancer cells and retrieve hepatocyte-specific characteristics including hepatocyte-like morphology;global expression pattern of genes and specific biomarkers of hepatocytes;and the unique hepatic functions of albumin (ALB)secretion,glycogen synthesis,low-density lipoprotein (LDL)uptake,urea production, cytochrome P450 enzymes induction and drug metabolism.Intratumoral injection of these three factors efficiently shrank patientderived tumor xenografts and reprogrammed HCC cells in vivo.Most importantly,transplantation of rHeps in the liver of fumarylacetoacetate hydrolase-deficient (Fah^-/-)mice led to the reconstruction of hepatic lobules and the restoration of hepatic function.Mechanistically,exogenous expression of HNF1A,HNF4A and FOXA3 in HCC cells initiated the endogenous expression of numerous hepatocyte nuclear factors,which promoted the conversion of HCC cells to hepatocyte-like cells.Collectively,our results indicate the successful conversion of hepatoma cells to hepatocyte-like cells,not only extending our current knowledge of cell reprogramming but also providing a route towards a novel therapeutic strategy for cancer. | Zhuo Cheng Zhiying He Yongchao Cai Cheng Zhang Gongbo Fu Hengyu Li Wen Sun Changcheng Liu Xiuliang Cui Beifang Ning Daimin Xiang Tengfei Zhou Xiaofeng Li Weifen Xie Hongyang Wang Jin Ding | 2019 | Cell Research2019，29，2： | 3 |
| 3 | Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures显示文摘抗菌素编码 anti-CRISPR suppressors 抵抗他们的细菌的主人的 CRISPR/Cas 免疫,因此便于他们的幸存和复制。以前的研究显示出那二编码噬菌体的 anti-CRISPR 蛋白质, AcrF1 和 AcrF2,如果由由 Csy 监视建筑群阻止目标 DNA 识别的 Pseudomonas aeruginosa 的 CRISPR/Cas 系统,而是精确位于下面机制是未知的,压制类型。这里我们在场 Csy 建筑群一定的 AcrF1/2 的结构由 cryo 他们决定了单个粒子的重建。由结构的分析,我们发现 AcrF1 由防碍在 DNA 目标海滨和 crRNA 分隔符之间的基础配对禁止 Csy 建筑群的目标 DNA 识别。另外,当个别地工作或与 AcrF2 合作时, AcrF1 的多重拷贝与不同模式绑在 Csy 建筑群,它可能排除通过不同机制有约束力的目标 DNA。和以前的报告,我们为二 anti-CRISPR suppressors, AcrF1 和 AcrF2 提供一种全面工作情形,它由指向 Csy 监视建筑群的沉默 CRISPR/Cas 免疫。 | Ruchao Peng Ying Xu Tengfei Zhu Ningning Li Jianxun Qi Yan Chai Min Wu Xinzheng Zhang Yi Shi Peiyi Wang Jiawei Wang Ning Gao George Fu Gao | 2017 | Cell Research2017，27，7： | 3 |
| 4 | Irradiation effects on multilayered W/ZrO 2 film under 4MeV Au ions显示文摘 | Hongwei Wang Yuan Gao Engang Fu Tengfei Yang Jianming Xue Sha Yan Paul K. Chu Yugang Wang | 2014 | 2014 (1-3)2014，，1： | 1 |
| 5 | Ribonuclease H-like gene SMALL GRAIN2 regulates grain size in rice through brassinosteroid signaling pathway显示文摘Grain size is a key agronomic trait that determines the yield in plants.Regulation of grain size by brassinosteroids(BRs)in rice has been widely reported.However,the relationship between the BR signaling pathway and grain size still requires further study.Here,we isolated a rice mutant,named small grain2(sg2),which displayed smaller grain and a semi-dwarf phenotype.The decreased grain size was caused by repressed cell expansion in spikelet hulls of the sg2 mutant.Using map-based cloning combined with a Mut Map approach,we cloned SG2,which encodes a plant-specific protein with a ribonuclease H-like domain.SG2 is a positive regulator downstream of GLYCOGEN SYNTHASE KINASE2(GSK2)in response to BR signaling,and its mutation causes insensitivity to exogenous BR treatment.Genetical and biochemical analysis showed that GSK2 interacts with and phosphorylates SG2.We further found that BRs enhance the accumulation of SG2 in the nucleus,and subcellular distribution of SG2 is regulated by GSK2 kinase activity.In addition,Oryza sativa OVATE family protein 19(OsOFP19),a negative regulator of grain shape,interacts with SG2 and plays an antagonistic role with SG2 in controlling gene expression and grain size.Our results indicated that SG2 is a new component of GSK2-related BR signaling response and regulates grain size by interacting with Os OFP19. | Yunshuai Huang Hui Dong Changling Mou Ping Wang Qixian Hao Min Zhang Hongmin Wu Fulin Zhang Tengfei Ma Rong Miao Kai Fu Yaping Chen Ziyan Zhu Cheng Chen Qikai Tong Zhuoran Wang Shirong Zhou Xi Liu Shijia Liu Yunlu Tian Ling Jiang Jianmin Wan | 2022 | Journal of Integrative Plant Biology2022，64，10： | 1 |
| 6 | Enriched endoplasmic reticulum-mitochondria interactions result in mitochondrial dysfunction and apoptosis in oocytes from obese mice显示文摘Background: Maternal obesity alters oocytes and subsequent fetal metabolism. An increasing number of studies have shown that the endoplasmic reticulums(ER) or mitochondria have important effects on oocyte quality, but there has been no study of the effect of mitochondria-associated ER membranes(MAMs) on oocyte quality. The present study was designed to assess whether the level of MAM and MAM-related proteins were different in oocytes from obese and control mice.Results: First, oocytes from mice with high-fat-diet(HFD)-induced obesity had higher levels(either greater numbers or a higher proportion for the same numbers) of MAM than oocytes from control mice. The abundance of MAM-related proteins in oocytes from obese mice was significantly greater at both the messenger RNA and protein levels, including inositol 1,4,5-trisphosphate receptor, type 1(IP3R1), inositol 1,4,5-trisphosphate receptor,type 2(IP3 R2) and phosphofurin acidic cluster sorting protein 2(PACS-2). Further, there was an increase in mitochondrial Ca^(2+)([Ca^(2+)]_m) which was associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. Down-regulation of MAM-related protein IP3R1 in oocytes from obese mice decreased [Ca^(2+)]_mand apoptosis and improved cytoplasmic maturation but did not reduce the overal MAM level. However, down-regulating MAM-related protein PACS-2 in oocytes from obese mice did reduce the level of MAM and [Ca^(2+)]_m, which decreased the rate of apoptosis and improved cytoplasmic maturation of oocytes from obese mice.Conclusions: It is possible that enriched MAM could increase [Ca^(2+)]_m, and this increase has been found to be associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. This finding suggests a novel therapeutic target for obesity-induced oocyte defects. | Lihong Zhao Tengfei Lu Lei Gao Xiangwei Fu Shien Zhu Yunpeng Hou | 2018 | Journal of Animal Science and Biotechnology2018，9，1： | 1 |
| 7 | A partitioning based algorithm to fuzzy tri-cluster显示文摘 | Liu Yongli Yang Tengfei Fu Lili | 2015 | Mathematical Problems in Engineering2015，，12： | 1 |
| 8 | An improved binocular localization method for apple based on fruit detection using deep learning显示文摘Apple picking robot is now being developed as an alternative to hand picking due to a great demand for labor during apple harvest season.Accurate detection and localization of target fruit is necessary for robotic apple picking.Detection accuracy has a great influence on localization results.Although current researches on detection and localization of apples using traditional image algorithms can obtain good results under laboratory conditions,it is difficult to accurately detect and locate objects in natural field with complex environments.With the rapid development of artificial intelligence,accuracy of apple detection based on deep learning has been significantly improved.Therefore,a deep learningbased method was developed to accurately detect and locate the position of fruit.For different localization methods,binocular localization is a widely used localization method for its bionic principle and lower equipment cost.Hence,this paper proposed an improved binocular localization method for apple based on fruit detection using deep learning.First,apples of binocular images were detected by Faster R-CNN.After that,a segmentation based on chromatic aberration and chromatic aberration ratio was applied to segment apple and background pixels in bounding box of detected fruit.Furthermore,template matching with parallel polar line constraint was used to match apples in left and right images.Finally,two feature points on apples were selected to directly calculate three dimensional coordinates of feature points with the binocular localization principle.In this study,Faster R-CNN achieved an AP of 88.12%with an average detection speed of 0.32 s for an image.Meanwhile,standard deviation and localization precision of depth of two feature points on each apple were calculated to evaluate localization.Results showed that the average standard deviation and the average localization precision of 76 groups of datasets were 0.51 cm and 99.64%,respectively.Results indicated that the proposed improved binocular localization method is promising for fruit localization。 | Tengfei Li Wentai Fang Guanao Zhao Fangfang Gao Zhenchao Wu Rui Li Longsheng Fu Jaspreet Dhupia | 2023 | Information Processing in Agriculture2023，10，2： | 1 |
| 9 | Effect of silicon-doped calcium phosphate cement on angiogenesis based on controlled macrophage polarization显示文摘Vascularization is an important early indicator of osteogenesis involving biomaterials. Bone repair and new bone formation are associated with extensive neovascularization. Silicon-based biomaterials have attracted widespread attention due to their rapid vascularization. Although calcium phosphate cement (CPC) is a mature substitute for bone, the application of CPC is limited by its slow degradation and insufficient promotion of neovascularization. Calcium silicate (CS) has been shown to stimulate vascular endothelial proliferation. Thus, CS may be added to CPC (CPC–CS) to improve the biocompatibility and neovascularization of CPC. In the early phase of bone repair (the inflammatory phase), macrophages accumulate around the biomaterial and exert both anti- and pro-inflammatory effects. However, the effect of CPC–CS on macrophage polarization is not known, and it is not clear whether the effect on neovascularization is mediated through macrophage polarization. In the present study, we explored whether silicon-mediated macrophage polarization contributes to vascularization by evaluating the CPC–CS-mediated changes in the immuno-environment under different silicate ion contents both in vivo and in vitro. We found that the silicon released from CPC–CS can promote macrophage polarization into the M2 phenotype and rapid endothelial neovascularization during bone repair. Dramatic neovascularization and osteogenesis were observed in mouse calvarial bone defects implanted with CPC–CS containing 60% CS. These findings suggest that CPC–CS is a novel biomaterial that can modulate immune response, promote endothelial proliferation, and facilitate neovascularization and osteogenesis. Thus, CPC–CS shows potential as a bone substitute material. | Soomin Lee Zheng Li Dehua Meng Qinming Fei Libo Jiang Tengfei Fu Ze Wang Shuhao Liu Jian Zhang | 2021 | Acta Biochimica et Biophysica Sinica2021，53，11： | 0 |
| 10 | Comparison of three data mining methods in predicting 5-year survival of colorectal cancer patients显示文摘The prediction of colorectal cancer(CRC) survivability has always been a challenging research issue. Considering the importance of predicting CRC patients' survival rates, we compared the performance of three data mining methods: decision trees(DTs), artificial neural networks(ANNs) and support vector machines(SVMs), for predicting 5-year survival of CRC patients to assist clinicians in making treatment decisions. The CRC dataset used to build the prediction model comes from the surveillance, epidemiology, and end results(SEER) program. The 5-fold cross-validation and random forest algorithm were respectively utilized for measuring the model predictive accuracy and the importance of features. Experimental results show that the predictive accuracy of ANNs(0.73) and SVMs(0.75) were higher than that of DTs, and they also have the best result in the area under the receiver operating characteristic(ROC) curve(area under curve(AUC)=0.82). This result may indicate high predictive power of ANNs and SVMs for predicting 5-year survival of CRC patients. | Luo Yan Sun Yawei Fu Qunchao Xue Tengfei Zhou Ping | 2018 | The Journal of China Universities of Posts and Telecommunications2018，25，6： | 0 |
| 11 | Human endoderm stem cells reverse inflammation-related acute liver failure through cystatin SN-mediated inhibition of interferon signaling显示文摘Acute liver failure(ALF)is a life-threatening disease that occurs secondary to drug toxicity,infection or a devastating immune response.Orthotopic liver transplantation is an effective treatment but limited by the shortage of donor organs,the requirement for life-long immune suppression and surgical challenges.Stem cell transplantation is a promising alternative therapy for fulminant liver failure owing to the immunomodulatory abilities of stem cells.Here,we report that when transplanted into the liver,human endoderm stem cells(hEnSCs)that are germ layer-specific and nontumorigenic cells derived from pluripotent stem cells are able to effectively ameliorate hepatic injury in multiple rodent and swine drug-induced ALF models.We demonstrate that hEnSCs tune the local immune microenvironment by skewing macrophages/Kupffer cells towards an anti-inflammatory state and by reducing the infiltrating monocytes/macrophages and inflammatory T helper cells.Single-cell transcriptomic analyses of infiltrating and resident monocytes/macrophages isolated from animal livers revealed dramatic changes,including changes in gene expression that correlated with the change of activation states,and dynamic population heterogeneity among these cells after hEnSC transplantation.We further demonstrate that hEnSCs modulate the activation state of macrophages/Kupffer cells via cystatin SN(CST1)-mediated inhibition of interferon signaling and therefore highlight CST1 as a candidate therapeutic agent for diseases that involve over-activation of interferons.We propose that hEnSC transplantation represents a novel and powerful cell therapeutic treatment for ALF. | Yilin Xu Jinglin Wang Haozhen Ren Hao Dai Ying Zhou Xiongzhao Ren Yang Wang Sisi Feng Xiaogang Deng Jiaying Wu Tianlong Fu Tengfei Nie Haifeng He Tongkun Wei Bing Zhu Lijian Hui Bin Li Jing Wang Hongyan Wang Luonan Chen Xiaolei Shi Xin Cheng | 2023 | Cell Research2023，33，2： | 0 |
| 12 | P2P transaction method for distributed energy prosumers based on reputation value显示文摘Adding a reputation incentive system to peer-to-peer(P2P)energy transactions can encourage prosumers to regulate their trading behavior,which is important for ensuring the efficiency and reliability of P2P transactions.This study proposed a P2P transaction mechanism and game optimization model for prosumers involved in distributed energy sources considering reputation-value incentives.First,the deviation of P2P transactions and the non-consumption rate of distributed renewable energy in P2P transactions were established as indicators to quantify the influencing factors of the reputation value,and a reputation incentive model of P2P transactions for prosumers was constructed.Then,the penalty coefficient was applied to the cost function of the prosumers,and a non-cooperative game model of P2P transactions based on the complete information of multi-prosumers was established.Furthermore,the Nash equilibrium problem was transformed into a nonlinear optimization problem by constructing the modified optimal reaction function,and the Nash equilibrium solution of the game was obtained via a relaxation algorithm.Finally,the modified IEEE 33-node test system based on electricity market P2P and an IEEE 123-node test system were used to analyze and verify the cost and P2P participation of prosumers considering the reputation value.The results show that the addition of the reputation incentive system can encourage prosumers to standardize their interactive transaction behavior and actively participate in P2P transactions.It can also improve the operation efficiency of the power grid and promote the perfection of the P2P transaction mechanism. | Tao Jiang Ting Hua Hao Xiao Linbo Fu Wei Pei Tengfei Ma | 2023 | Global Energy Interconnection2023，6，3： | 0 |
| 13 | In vitro assembly of Ebola virus nucleocapsidlike complex expressed in E. coil显示文摘 | Ruchao Peng Tengfei Zhu Babayemi Olawale Oladejo Abednego Moki Musyoki Yingzi Cui Yi Shi Peiyi Wang George Fu Gao | 2016 | Protein & Cell2016，7，12： | 0 |
| 14 | Transmission of Carbapenem Resistance Between Human and Animal NDM-Positive Escherichia coli Strains显示文摘Although carbapenem use is prohibited in animals in China,carbapenem-resistant Escherichia coli(CREC),especially New Delhi metallo-β-lactamase(NDM)-producing strains,are widely prevalent in foodproducing animals.At present,the impact of livestock-associated CREC strains on human populations at the national level is unknown.Here,we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence.We also ascertain the blaNDMand blaKPCabundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin.Overall,631/29799(2.1%)clinical Escherichia coli(E.coli)isolates were identified as CREC.Multivariable analysis revealed that being male,an age below 1,an age between 13 and 18,provinces with greater chicken production,and provinces with higher pig production were associated with higher odds of CREC infection.In general,73.8%(n=45/61)of pig farms and 62.2%(n=28/45)of chicken farms had a blaNDMabundance of 1×10^(-5)to 1×10^(-3)and 1×10^(-3)to 1×10^(-2),respectively.Among all the Chinese NDM-positive E.coli(n=463)available at the National Center for Biotechnology Information(NCBI),the genomic analysis revealed that blaNDM-5and Inc X3 were the predominant carbapenemase gene-plasmid combination,while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels.All the findings suggest frequent CREC transmission between humans and animals,indicating that further discussions on the use of antibiotics in animals and humans are needed,both in China and across the globe. | Yingbo Shen Fupin Hu Yongqiang Wang Dandan Yin Lu Yang Yiqiang Chen Chunyan Xu Jiyun Li Junyao Jiang Xueyang Wang Yulin Fu Dongyan Shao Dejun Liu Tengfei Ma Chang Cai Zhangqi Shen Shaolin Wang Juan Li Rong Zhang Yuebin Ke Congming Wu Jianzhong Shen Timothy R.Walsh Yang Wang | 2022 | Engineering2022，8，8： | 0 |
| 15 | Research status and development directions of intelligent drilling fluid technologies显示文摘By reviewing the current status of drilling fluid technologies with primary intelligence features at home and abroad,the development background and intelligent response mechanisms of drilling fluid technologies such as variable density,salt response,reversible emulsification,constant rheology,shape memory loss prevention and plugging,intelligent reservoir protection and in-situ rheology control are elaborated,current issues and future challenges are analyzed,and it is pointed out that intelligent material science,nanoscience and artificial intelligence theory are important methods for future research of intelligent drilling fluid technology of horizontal wells with more advanced intelligent features of'self-identification,self-tuning and self-adaptation'.Based on the aforementioned outline and integrated with the demands from the drilling fluid technology and intelligent drilling fluid theory,three development suggestions are put forward:(1)research and develop intelligent drilling fluids responding to variable formation pressure,variable formation lithology and fluid,variable reservoir characteristics,high temperature formation and complex ground environmental protection needs;(2)establish an expert system for intelligent drilling fluid design and management;and(3)establish a real-time intelligent check and maintenance processing network. | JIANG Guancheng DONG Tengfei CUI Kaixiao HE Yinbo QUAN Xiaohu YANG Lili FU Yue | 2022 | Petroleum Exploration and Development2022，49，3： | 0 |
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