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| 1 | Identification of a novel coronavirus causing severe pneumonia in human:a descriptive study显示文摘Background:Human infections with zoonotic coronaviruses(CoVs),including severe acute respiratory syndrome(SARS)-CoV and Middle East respiratory syndrome(MERS)-CoV,have raised great public health concern globally.Here,we report a novel batorigin CoV causing severe and fatal pneumonia in humans.Methods:We collected clinical data and bronchoalveolar lavage(BAL)specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital,Hubei province,China.Nucleic acids of the BAL were extracted and subjected to next-generation sequencing.Virus isolation was carried out,and maximum-likelihood phylogenetic trees were constructed.Results:Five patients hospitalized from December 18 to December 29,2019 presented with fever,cough,and dyspnea accompanied by complications of acute respiratory distress syndrome.Chest radiography revealed diffuse opacities and consolidation.One of these patients died.Sequence results revealed the presence of a previously unknownβ-CoV strain in all five patients,with 99.8%to 99.9%nucleotide identities among the isolates.These isolates showed 79.0%nucleotide identity with the sequence of SARS-CoV(GenBank NC_004718)and 51.8%identity with the sequence of MERS-CoV(GenBank NC_019843).The virus is phylogenetically closest to a bat SARS-like CoV(SL-ZC45,GenBank MG772933)with 87.6%to 87.7%nucleotide identity,but is in a separate clade.Moreover,these viruses have a single intact open reading frame gene 8,as a further indicator of bat-origin CoVs.However,the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV,indicating that these viruses might use the same receptor.Conclusion:A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans. | Li-Li Ren Ye-Ming Wang Zhi-Qiang Wu Zi-Chun Xiang Li Guo Teng Xu Yong-Zhong Jiang Yan Xiong Yong-Jun Li Xing-Wang Li Hui Li Guo-Hui Fan Xiao-Ying Gu Yan Xiao Hong Gao Jiu-Yang Xu Fan Yang Xin-Ming Wang Chao Wu Lan Chen Yi-Wei Liu Bo Liu Jian Yang Xiao-Rui Wang Jie Dong Li Li Chao-Lin Huang Jian-Ping Zhao Yi Hu Zhen-Shun Cheng Un-Lin Liu Zhao-Hui Qian Chuan Qin Qi Jin Bin Cao Jian-Wei Wang | 2020 | Chinese Medical Journal2020，，9： | 99 |
| 2 | Practice guidelines for the pathological diagnosis of primary liver cancer: 2015 update显示文摘In 2010, a panel of Chinese pathologists reported the first expert consensus for the pathological diagnosis of primary liver cancers to address the many contradictions and inconsistencies in the pathological characteristics and diagnostic criteria for PLC. Since then considerable clinicopathological studies have been conducted globally, prompting us to update the practice guidelines for the pathological diagnosis of PLC. In April 18, 2014, a Guideline Committee consisting of 40 specialists from seven Chinese Societies(including Chinese Society of Liver Cancer, Chinese Anti-Cancer Association; Liver Cancer Study Group, Chinese Society of Hepatology, Chinese Medical Association; Chinese Society of Pathology, Chinese Anti-Cancer Association; Digestive Disease Group, Chinese Society of Pathology, Chinese Medical Association; Chinese Society of Surgery, Chinese Medical Association; Chinese Society of Clinical Oncology, Chinese Anti-Cancer Association; Pathological Group of Hepatobiliary Tumor and Liver Transplantation, Chinese Society of Pathology, Chinese Medical Association) was created for the formulation of the first guidelines for the standardization of the pathological diagnosis of PLC, mainly focusing on the following topics: gross specimen sampling, concepts and diagnostic criteria of small hepatocellular carcinoma(SHCC), microvascular invasion(MVI), satellite nodules,and immunohistochemical and molecular diagnosis. The present updated guidelines are reflective of current clinicopathological studies, and include a novel 7-point baseline sampling protocol, which stipulate that at least four tissue specimens should be sampled at the junction of the tumor and adjacent liver tissues in a 1:1 ratio at the 12, 3, 6 and 9 o'clock reference positions. For the purposes of molecular pathological examination, at least one specimen should be sampled at the intratumoral zone, but more specimens should be sampled for tumors harboring different textures or colors. Specimens should be sampled at both adjacent and distant peritumoral liver tissues or the tumor margin in order to observe MVI, satellite nodules and dysplastic foci/nodules distributed throughout the background liver tissues. Complete sampling of whole SHCC ≤ 3 cm should be performed to assess its biological behavior, and in clinical practice, therapeutic borders should be also preserved, even in SHCC. The diagnostic criteria of MVI and satellite nodules, immunohistochemical panels, as well as molecular diagnostic principles, such as clonal typing, for recurrent HCC and multinodule HCC were also proposed and recommended. The standardized process of pathological examination is aimed at ensuring the accuracy of pathological PLC diagnoses as well as providing a valuable frame of reference for the clinical assessment of tumor invasive potential, the risk of postoperative recurrence, long-term survival, and the development of individualized treatment regimens. The updated guidelines could ensure the accuracy of pathological diagnoses of PLC, and provide a valuable frame of reference for its clinical assessment. | Wen-Ming Cong Hong Bu Jie Chen Hui Dong Yu-Yao Zhu Long-Hai Feng Jun Chen 无 | 2016 | World Journal of Gastroenterology2016，22，42： | 81 |
| 3 | The Genome of Dendrobium officinale Illuminates the Biology of the Important Traditional Chinese Orchid Herb显示文摘 | Liang Yan Xiao Wang Hui Liu Yang Tian Jinmin Lian Ruijuan Yang ShumeiHao Xuanjun Wang Shengchao Yang Qiye Li Shuai Qi Ling Kui Moses Okpekum Xiao Ma Jiajin Zhang Zhaoli Ding Guojie Zhang Wen Wang Yang Dong Jun Sheng | 2015 | Molecular Plant2015，8，6： | 64 |
| 4 | Artificial intelligence in healthcare:past,present and future显示文摘Artificial intelligence(AI)aims to mimic human cognitive functions.It is bringing a paradigm shift to healthcare,powered by increasing availability of healthcare data and rapid progress of analytics techniques.We survey the current status of AI applications in healthcare and discuss its future.AI can be applied to various types of healthcare data(structured and unstructured).Popular AI techniques include machine learning methods for structured data,such as the classical support vector machine and neural network,and the modern deep learning,as well as natural language processing for unstructured data.Major disease areas that use AI tools include cancer,neurology and cardiology.We then review in more details the AI applications in stroke,in the three major areas of early detection and diagnosis,treatment,as well as outcome prediction and prognosis evaluation.We conclude with discussion about pioneer AI systems,such as IBM Watson,and hurdles for real-life deployment of AI. | Fei Jiang Yong Jiang Hui Zhi Yi Dong Hao Li Sufeng Ma Yilong Wang Qiang Dong Haipeng Shen Yongjun Wang | 2017 | Stroke & Vascular Neurology2017，2，4： | 76 |
| 5 | Differentiation of bone marrow-derived mesenchymal stem cells from diabetic patients into insulin-producing cells in vitro显示文摘Bckground 干细胞,有能力区分进生产胰岛素的房间(欧洲电脑生产厂商) ,将为移植提供小岛房间的潜在地无限的来源并且减轻可获得性和 allogeneic 拒绝的主要限制。因此,干细胞的利用为糖尿病 mellitus (DM ) 正在成为最有希望的治疗。这里,我们学习了糖尿病的病人的骨头的区别能力导出髓的间充质的干细胞( MSC )并且测试了使用 MSC 的可行性因为房间 replacement.Methods 骨头导出髓的 MSC 从 10 个 DM 病人被获得( 5 打 1 DM , 5 打 2 DM )并且在一个三阶段的协议下面导致了到欧洲电脑生产厂商。MSC 的代表性的房间表面抗原表示侧面被流动 cytometric 分析分析。反向的抄写聚合酶链反应(RT-PCR ) 被执行检测与胰腺的房间开发和功能有关的多重基因。欧洲电脑生产厂商的身份被形态学, ditizone 染色和 immunocytochemistry 的分析说明。由这些房间的胰岛素的版本被 MSC 的 immunoradioassay.Results 流动 cytometric 分析在 3 显示出的经过证实这些房间表示了 CD29 (98.28%) 的高水平, CD44 (99.56%) 和 CD106 (98.34%) 。典型像小岛的房间簇在协议(18 天) 的结束被观察。染色的 Ditizone 和为胰岛素的 immunohistochemistry 两个都是积极的。在舞台 2 点的这些区分的房间(10 天) 表示了 nestin,胰腺的十二指肠的 homeobox-1 (PDX-1 ) , Neurogenin3, Pax4,胰岛素, glucagon,但是(18 天) 在舞台 3 点,我们观察了 PDX-1 的高表示,胰岛素, glucagon。胰岛素被这些房间以从 DM 病人的 .Conclusions 骨头导出髓的 MSC 能在 vitro 在某些条件下面区分进功能的欧洲电脑生产厂商的一种调整方式(P0.05 ) 响应葡萄糖刺激的不同集中分泌。用糖尿病的病人的自己的骨头,导出髓的 MSC 将作为为房间代替的自体同源的欧洲电脑生产厂商的来源是可行的。 | SUN Yu CHEN Li HOU Xin-guo HOU Wei-kai DONG Jian-jun SUN Lei TANG Kuan-xiao WANG Bin SONG Jun LI Hui WANG Ke-xin | 2007 | Chinese Medical Journal2007，，9： | 57 |
| 6 | Changes in heavy metal contents in animal feeds and manures in an intensive animal production region of China显示文摘The 360 feed and manure samples were collected from 150 animal farms in Jiangsu Province, China and analyzed for heavy metals. Concentrations of Zn and Cu in animal feeds were 15.9–2041.8 and undetected–392.1 mg/kg respectively, while Hg, As, Pb, Cd, and Cr in all feeds were below 10 mg/kg. Concentrations of Cu, Zn, and Cr in animal manures were 8.4–1726, 39.5–11379, and 1.0–1602 mg/kg respectively, while As, Cd, Hg, and Pb were < 10 mg/kg. The concentration of Cu, Zn, As and Cr in animal feed and manure were positively correlated(p < 0.001), but the Cd, Hg, and Pb were not statistically correlated between the feed and the manure. Concentrations of Cu and Zn were highest in pig feed and manure, followed by poultry and dairy feeds and manures. During 1990– 2008, Cu, Zn, As, Cr, Cd contents increased by 771%, 410%, 420%, 220%, and 63% in pig manure, 212%, 95%, 200%, 791%, and –63% in dairy manure, and 181%, 197%, 1500%, 261, and 196% in poultry manure. Most of the increases occurred from 2002 to 2008, which reflects the extensive use of feed additives after 2002. In contrast, Pb and Hg in manures continuously decreased from 1990 to 2008. The results suggest that the heavy metal contents in animal manure have been greatly increased over 18 years and the contribution of manures to soil should be considered. | Hui Wang Yuanhua Dong Yunya Yang Gurpal S.Toor Xumei Zhang | 2013 | Journal of Environmental Sciences2013，25，12： | 54 |
| 7 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 8 | Effect of Microstructure Refinement on the Strength and Toughness of Low Alloy Martensitic Steel显示文摘马氏体的微观结构在熄灭,有从 6 亩 m 到 199 亩 m 的优先的奥氏体谷物尺寸的 tempered 17CrNiMo6 钢被光金属组织学( OM )描绘了,与各种各样的优先的奥氏体谷物尺寸扫描电子显微镜学( SEM )和传动电子显微镜学( TEM ) .Theyield 强度和钢的坚韧被测试并且与微观结构特征相关。结果证明在 17CrNiMo6 钢的优先的奥氏体谷物尺寸和马氏体的包尺寸与收益强度跟随一种 Hall-Petch 关系。当优先的奥氏体谷物尺寸从 199 亩 m 被精制到 6 亩 m 时,收益强度由 235 MPa 增加了,当在 77 K 的 Charpy U槽口冲击能多于 8times 改善了时,显示那微观结构精炼在比在增加强度改进抵抗到劈开骨折是更有效的。破裂表面暗示为劈开骨折的联合起来的裂缝路径作为是包被识别。 | Chunfang WANG Maoqiu WANG Jie SHI Weijun HUI Han DONG | 2007 | Journal of Materials Science & Technology2007，23，5： | 50 |
| 9 | Berberine reverses free-fatty-acid-induced insulin resistance in 3T3-L1 adipocytes through targeting IKKβ显示文摘AIM: To investigate the effects and molecular mechanisms of berberine on improving insulin resistance induced by free fatty acids (FFAs) in 3T3-L1 adipocytes. METHODS: The model of insulin resistance in 3T3-L1 adipocytes was established by adding palmic acid (0.5 mmol/L) to the culture medium. Berberine treatment was performed at the same time. Glucose uptake rate was determined by the 2-deoxy-[3H]-D-glucose method. The levels of IkB kinase beta (IKKβ) Ser181 phosphorylation, insulin receptor substrate-1(IRS-1) Ser307 phosphorylation, expression of IKKβ, IRS-1, nuclear transcription factor kappaB p65 (NF-κB p65), phosphatidylinositol-3-kinase p85 (PI-3K p85) and glucose transporter 4 (GLUT4) proteins were detected by Western blotting. The distribution of NF-κB p65 proteins inside the adipocytes was observed through confocal laser scanning microscopy (CLSM). RESULTS: After the intervention of palmic acid for 24 h, the insulin-stimulated glucose transport in 3T3-L1 adipocytes was inhibited by 67%. Meanwhile, the expression of IRS-1 and PI-3K p85 protein was reduced, while the levels of IKKβ Ser181 and IRS-1 Ser307 phosphorylation, and nuclear translocation of NF-κB p65 protein were increased. However, the above indexes, which indicated the existence of insulin resistance, were reversed by berberine although the expression of GLUT4, IKKβ and total NF-κB p65 protein were not changed during this study. CONCLUSION: Insulin resistance induced by FFAs in 3T3-L1 adipocytes can be improved by berberine. Berberine reversed free-fatty-acid-induced insulin resistance in 3T3-L1 adipocytes through targeting IKKβ. | Ping Yi Fu-Er Lu Li-Jun Xu Guang Chen Hui Dong Kai-Fu Wang | 2008 | World Journal of Gastroenterology2008，14，6： | 50 |
| 10 | Effect of artificial aeration on the performance of vertical-flow constructed wetland treating heavily polluted river water显示文摘Three lab-scale vertical-flow constructed wetlands(VFCWs),including the non-aerated(NA),intermittently aerated(IA) and continuously aerated(CA) ones,were operated at different hydraulic loading rates(HLRs) to evaluate the effect of artificial aeration on the treatment efficiency of heavily polluted river water.Results indicated that artificial aeration increased the dissolved oxygen(DO) concentrations in IA and CA,which significantly favored the removal of organic matter and NH 4 +-N.The DO grads caused by intermittent aeration formed aerobic and anoxic regions in IA and thus promoted the removal of total nitrogen(TN).Although the removal efficiencies of COD Cr,NH 4 +-N and TN in the three VFCWs all decreased with an increase in HLR,artificial aeration enhanced the reactor resistance to the fluctuation of pollutant loadings.The maximal removal efficiencies of COD Cr,NH 4 +-N and total phosphorus(TP)(i.e.,81%,87% and 37%,respectively) were observed in CA at 19 cm/day HLR,while the maximal TN removal(i.e.,57%) was achieved in IA.Although the improvement of artificial aeration on TP removal was limited,this study has demonstrated the feasibility of applying artificial aeration to VFCWs treating polluted river water,particularly at a high HLR. | Huiyu Dong Zhimin Qiang Tinggang Li Hui Jin Weidong Chen | 2012 | Journal of Environmental Sciences2012，24，4： | 38 |
| 11 | Developing the Science Product Algorithm Testbed for Chinese Next-Generation Geostationary Meteorological Satellites:Fengyun-4 Series显示文摘Fengyun-4A(FY-4A), the first of the Chinese next-generation geostationary meteorological satellites, launched in2016, offers several advances over the FY-2: more spectral bands, faster imaging, and infrared hyperspectral measurements. To support the major objective of developing the prototypes of FY-4 science algorithms, two science product algorithm testbeds for imagers and sounders have been developed by the scientists in the FY-4 Algorithm Working Group(AWG). Both testbeds, written in FORTRAN and C programming languages for Linux or UNIX systems, have been tested successfully by using Intel/g compilers. Some important FY-4 science products, including cloud mask, cloud properties, and temperature profiles, have been retrieved successfully through using a proxy imager, Himawari-8/Advanced Himawari Imager(AHI), and sounder data, obtained from the Atmospheric Infra Red Sounder, thus demonstrating their robustness. In addition, in early 2016, the FY-4 AWG was developed based on the imager testbed—a near real-time processing system for Himawari-8/AHI data for use by Chinese weather forecasters.Consequently, robust and flexible science product algorithm testbeds have provided essential and productive tools for popularizing FY-4 data and developing substantial improvements in FY-4 products. | Min MIN Chunqiang WU Chuan LI Hui LIU Na XU Xiao WU Lin CHEN Fu WANG Fenglin SUN Danyu QIN Xi WANG Bo LI Zhaojun ZHENG Guangzhen CAO Lixin DONG | 2017 | Journal of Meteorological Research2017，31，4： | 35 |
| 12 | Berberine inhibits hepatic gluconeogenesis via the LKB1-AMPK-TORC2 signaling pathway in streptozotocin-induced diabetic rats显示文摘AIM: To investigate the molecular mechanisms of berberine inhibition of hepatic gluconeogenesis in a diabetic rat model.METHODS: The 40 rats were randomly divided into five groups. One group was selected as the normal group. In the remaining groups(n = 8 each), the rats were fed on a high-fat diet for 1 mo and received intravenous injection of streptozotocin for induction of the diabetic models. Berberine(156 mg/kg per day)(berberine group) or metformin(184 mg/kg per day)(metformin group) was intragastrically administered to the diabetic rats and 5-aminoimidazole-4-carboxamide1-β-D-ribofuranoside(AICAR)(0.5 mg/kg per day)(AICAR group) was subcutaneously injected to the diabetic rats for 12 wk. The remaining eight diabetic rats served as the model group. Fasting plasma glucose and insulin levels as well as lipid profile were tested.The expressions of proteins were examined by western blotting. The nuclear translocation of CREB-regulated transcription co-activator(TORC)2 was observed by immunohistochemical staining. RESULTS: Berberine improved impaired glucose tolerance and decreased plasma hyperlipidemia. Moreover, berberine decreased fasting plasma insulin and homeostasis model assessment of insulin resistance(HOMA-IR). Berberine upregulated protein expression of liver kinase(LK)B1, AMP-activated protein kinase(AMPK) and phosphorylated AMPK(p-AMPK). The level of phophorylated TORC2(p-TORC2) protein in the cytoplasm was higher in the berberine group than in the model group, and no significant difference in total TORC2 protein level was observed. Immunohistochemical staining revealed that more TORC2 was localized in the cytoplasm of the berberine group than in the model group. Moreover, berberine treatment downregulated protein expression of the key gluconeogenic enzymes(phosphoenolpyruvate carboxykinase and glucose-6-phosphatase) in the liver tissues. CONCLUSION: Our findings revealed that berberine inhibited hepatic gluconeogenesis via the regulation of the LKB1-AMPK-TORC2 signaling pathway. | Shu-Jun Jiang Hui Dong Jing-Bin Li Li-Jun Xu Xin Zou Kai-Fu Wang Fu-Er Lu Ping Yi | 2015 | World Journal of Gastroenterology2015，21，25： | 33 |
| 13 | Exploring the history of cultural exchange in prehistoric Eurasia from the perspectives of crop diffusion and consumption显示文摘The history of cultural exchange in prehistoric Eurasia(CEPE) has been widely investigated. Based on archaeological evidence, this process is thought to date back to at least the early Bronze Age, although details about timings and routes remain unclear. It is likely that CEPE promoted the spread and exchange of crops that originated in different parts of Eurasia; since these remains can be definitely identified and directly dated, they provide ideal research materials to explore the history of CEPE. In this paper, we review the available archaeobotanical evidence and direct radiocarbon dates for crop remains, alongside carbon isotopic data from human bones unearthed from prehistoric sites in Eurasia, in order to investigate the history of the spread of millet crops, and wheat and barley, that were first domesticated in the eastern and western parts of Eurasia during prehistoric times.In combination with other archaeological evidences, we discuss the history of CEPE. Our results suggest that wheat and barley were domesticated in western Asia around 10500 a BP, spread into Europe and western Central Asia before 8000 a BP, and reaching eastern Central Asia and northwestern China between 4500 and 4000 a BP. Data show that both broomcorn and foxtail millet were domesticated in eastern Asia before 7700 a BP, spread into eastern Central Asia between 4500 and 4000 a BP, and into western Asia and Europe prior to 3500 a BP. Wheat, barley, and millet crops were first utilized together in eastern Kazakhstan within Central Asia around 4400 a BP, the region where earliest CEPE is likely to have taken place. These crops were mixedly used mainly in eastern central Asia and northwest China between 4500 and 3500 a BP, and then across the Eurasia before 2200 a BP. The results of this study suggest that transcontinental CEPE might have been initiated during the fifth millennium, before intensifying during the Bronze Age to lay the foundations for the creation of the ancient Silk Road during the Han Dynasty(between 202 BC and 220AD). | DONG GuangHui YANG YiShi HAN JianYe WANG Hui CHEN FaHu | 2017 | Science China Earth Sciences2017，60，6： | 30 |
| 14 | Structures of EV71 RNA-dependent RNA polymerase in complex with substrate and analogue provide a drug target against the hand-foot-and-mouth disease pandemic in China显示文摘Enterovirus 71(EV71),one of the major causative agents for hand-foot-and-mouth disease(HFMD),has caused more than 100 deaths among Chinese children since March 2008.The EV71 genome encodes an RNAdependent RNA polymerase(RdRp),denoted 3D^(pol),which is central for viral genome replication and is a key target for the discovery of specific antiviral therapeutics.Here we report the crystal structures of EV71 RdRp(3D^(pol))and in complex with substrate guanosine-5'-triphosphate and analog 5-bromouridine-5'-triphosphate best to 2.4Åresolution.The structure of EV71 RdRp(3D^(pol))has a wider open thumb domain compared with the most closely related crystal structure of poliovirus RdRp.And the EV71 RdRp(3D^(pol))complex with GTP or Br-UTP bounded shows two distinct movements of the polymerase by substrate or analogue binding.The model of the complex with the template:primer derived by superimposition with foot-and-mouth disease virus(FMDV)3D/RNA complex reveals the likely recognition and binding of template:primer RNA by the polymerase.These results together provide a molecular basis for EV71 RNA replication and reveal a potential target for anti-EV71 drug discovery. | Yang Wu Zhiyong Lou Yi Miao Yue Yu Hui Dong Wei Peng Mark Bartlam Xuemei Li Zihe Rao | 2010 | Protein & Cell2010，1，5： | 30 |
| 15 | Therapy and prognostic features of primary clear cell carcinoma of the liver显示文摘AIM:To clarify the therapeutic strategies and prognosis factors of primary clear cell carcinoma of the liver(PCCCL) . METHODS:The clinical pathological data of 64 patients with PCCCL treated with hepatectomy in our hospital from January 2000 to January 2006 were analyzed retrospectively.The patients were divided into two groups to make treatment analysis:curative resection only(n=40) ;and curative resection and postoperative chemotherapy with calcium folinate and tegafur(n= 24) .Meanwhile,the PCCCL patients were subdivided into two subgroups on the basis of the proportion of clear cells in the tumor for pathological analysis.There were 36 cases in subgroup A for which the proportion of clear cells was more than 70%,and 28 cases in subgroup B for which the proportion was less or equal to 70%,comparing analysis of median survival time of the counterpart groups.Univariate and multivariate analyses were performed to examine factors that affect-ed clinical prognosis,recurrence and metastasis. RESULTS:Median survival period of the curative surgery group was 38 mo,while the counterpart was 41 mo.Median survival period for group A was 41 mo,while group B was 19 mo.The Kaplan-Meier method showed that capsule formation,preoperative liver function,hepatitis C virus infection,large vascular invasion and multiple tumor occurrences were related to disease-free survival.Cox regression analysis showed that the clear cell ratio,capsule formation,preoperative liver function and large vascular invasion were independent risk factors for overall survival. CONCLUSION:Postoperative chemotherapy has no obvious effect on survival of patients with PCCCL. Clear cell ratio,capsule formation,preoperative liver function,and vascular invasion were independent risk factors for prognosis. | Ji, Sheng-Pu Li, Qiang Dong, Hui | 2010 | World Journal of Gastroenterology2010，16，6： | 28 |
| 16 | Surgicopathological classification of hepatic space-occupying lesions:A single-center experience with literature review显示文摘Accompanying rapid developments in hepatic surgery,the number of surgeries and identifications of histological types of primary hepatic space-occupying lesions (PHSOLs) have increased dramatically.This has led to many changes in the surgicopathological spectrum of PHSOLs,and has contributed to a theoretical basis for modern hepatic surgery and oncological pathology.Between 1982 and 2009 at the Eastern Hepatobiliary Surgery Hospital (EHBH) in Shanghai,31 901 patients underwent surgery and were diagnosed as having a PHSOL.In this paper,we present an analysis of the PHSOL cases at the EHBH for this time period,along with results from a systematic literature review.We describe a surgicopathological spectrum comprising more than 100 types of PHSOLs that can be stratified into three types:tumor-like,benign,and malignant.We also stratified the PHSOLs into six subtypes derived from hepatocytes;cholangiocytes;vascular,lymphoid and hemopoietic tissues;muscular,fibrous and adipose tissues;neural and neuroendocrine tissues;and miscellaneous tissues.The present study provides a new classification system that can be used as a current reference for clinicians and pathologists to make correct diagnoses and differential diagnoses among various PHSOLs. | Wen-Ming Cong Hui Dong Lu Tan Xu-Xu Sun Meng-Chao Wu | 2011 | World Journal of Gastroenterology2011，17，19： | 25 |
| 17 | Neighbor Sum Distinguishing Total Colorings of Graphs with Bounded Maximum Average Degree显示文摘A proper [h]-total coloring c of a graph G is a proper total coloring c of G using colors of the set [h]={1,2,...,h}.Letw(u) denote the sum of the color on a vertex u and colors on all the edges incident to u.For each edge uv∈E(G),if w(u)≠w(v),then we say the coloring c distinguishes adjacent vertices by sum and call it a neighbor sum distinguishing [h]-total coloring of G.By tndi(G),we denote the smallest value h in such a coloring of G.In this paper,we obtain that G is a graph with at least two vertices,if mad(G)<3,then tndi∑(G)≤k+2 where k=max{Δ(G),5}.It partially con?rms the conjecture proposed by Pil′sniak and Wozniak. | Ai Jun DONG Guang Hui WANG | 2014 | Acta Mathematica Sinica,English Series2014，30，4： | 24 |
| 18 | Relationship between cytokines gene polymorphism and susceptibility to hepatitis B virus intrauterine infection显示文摘Background The influences of genomic background are confirmed in more diseases. Immunologic tolerance after intrauterine infection of hepatitis B virus is considered to occur in T cells. Cytokines work effectively in eliminating virus by immune system after hepatitis B virus infection. To explore the relationship between cytokines (tumor necrosis factor-α, interferon-γ, interleukin-4 and interleukin-10), which expressed abnormal quantity in the peripheral blood to intrauterine hepatitis B virus infectious children, gene single nucleotide polymorphism (SNP) and susceptibility to hepatitis B virus intrauterine infection. Methods This is a cross sectional study of molecular clinical epidemiology. The subjects in this study were selected from outpatients of hepatitis B vaccine follow-up special clinics of our hospital in the period. According to intrant criteria, the high risk children of hepatitis B virus (HBV) intrauterine infection were divided into immune failure group (group Ⅰ); and immune effective group (group Ⅱ) and non high risk children belonged to the control group. Four gene SNP sites of TNF-α -238, IFN-γ +874, IL-4 -590 and IL-10 -1082 were determined by real-time quantitative fluorescent polymerase chain reaction (PCR). Results The significant differences of TNF-α -238 A allele frequency were found between group Ⅰ and groupⅡ (χ2=6.797,P<0.05) and between groupⅠ and the control group (χ2=9.513,P<0.05). No evident differences of TNF-α -238 A were found between group Ⅱ and control group (χ2=0.047,P>0.05); the significant differences of IFN-γ +874 A allele frequency were found between groupⅠ and groupⅡ(χ2=7.238,P<0.05), and between groupⅠ and the control group (χ2=5.199,P<0.05). No evident differences were found between groupⅡ and the control group (χ2=0.602,P>0.05); the significant differences of IL-4 -590 C/T allele frequency were not found between groupⅠand group Ⅱ(χ2=0.632,P>0.05), also groupⅠ and the control group (χ2=0.584,P>0.05), and the groupⅡ and the control group (χ2=0.004,P>0.05)respectively; The significant differences of IL-10 -1082 G allele frequency were found between groupⅡ and groupⅠ (χ2=10.359,P<0.001), and between groupⅡ and the controls (χ2=35.418,P<0.001), but the significant differences were not found between groupⅠand the control group (χ2=1.759,P>0.05). Conclusions This study suggested the possibility that the TNF-α -238 A allele and IFN-γ +874 A allele were associated with HBV intrauterine infection. There was no evident relationship between IL-4 -590 C/T allele SNP and susceptibility to HBV intrauterine infection, but the IL-10 -1082 G allele was associated with preventive efficacy to HBV intrauterine infection. | ZHU Qi-rong GE Yan-ling GU Shao-qing YU Hui WANG Jian-she GU Xin-huan FEI Lin-e DONG Zuo-quan | 2005 | Chinese Medical Journal2005，，19： | 21 |
| 19 | A novel method for purification of recombinant adeno-associated virus vectors on a large scale显示文摘A novel method for recombinant adeno-associ-ated virus (rAAV) purification on large scale is described. The method involves three steps, including chloroform treatment, PEG/NaCl precipitation and chloroform extraction. The whole procedure can be performed in four hours. Using this purification method, we can reproducibly obtain, from 4 × 109 of proviral cells cultured in roller bottles, purified rAAV-GFP stocks with titers of around 5×1013 particles/mL and purity greater than 95%. The infectious titers of the vector stocks were up to 2×1012 TU/mL, thus particle-to-infectivity rate was about 25. Under an electronic microscope, most rAAV particles appeared full and a few were in intermediate form. Empty particles were rarely seen. The purified rAAV-GFP stocks have been successfully used in in vitro and in vivo transfection experiments. Therefore, this new method offers a simple, rapid and cost-effective way for large-scale rAAV purification. | WU Xiaobing DONG Xiaoyan WU Zhijian CAO Hui NIU Dongbin QU Jianguo WANG Hong HOU Yunde | 2001 | Chinese Science Bulletin2001，46，6： | 21 |
| 20 | A broadband achromatic metalens array for integral imaging in the visible显示文摘Integral imaging is a promising three-dimensional(3D)imaging technique that captures and reconstructs light field information.Microlens arrays are usually used for the reconstruction process to display 3D scenes to the viewer.However,the inherent chromatic aberration of the microlens array reduces the viewing quality,and thus,broadband achromatic imaging remains a challenge for integral imaging.Here,we realize a silicon nitride metalens array in the visible region that can be used to reconstruct 3D optical scenes in the achromatic integral imaging for white light.The metalens array contains 60×60 polarization-insensitive metalenses with nearly diffraction-limited focusing.The nanoposts in each high-efficiency(measured as 47%on average)metalens are delicately designed with zero effective material dispersion and an effective achromatic refractive index distribution from 430 to 780 nm.In addition,such an achromatic metalens array is composed of only a single silicon nitride layer with an ultrathin thickness of 400 nm,making the array suitable for on-chip hybrid-CMOS integration and the parallel manipulation of optoelectronic information.We expect these findings to provide possibilities for full-color and aberration-free integral imaging,and we envision that the proposed approach may be potentially applicable in the fields of high-power microlithography,high-precision wavefront sensors,virtual/augmented reality and 3D imaging. | Zhi-Bin Fan Hao-Yang Qiu Han-Le Zhang Xiao-Ning Pang Li-Dan Zhou Lin Liu Hui Ren Qiong-Hua Wang Jian-Wen Dong | 2019 | Light(Science & Applications)2019，8，1： | 21 |
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