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| 1 | Exposing Copy-Paste-Blur Forgeries Based on Color Coherence显示文摘 | WANG Bo KONG Xiangwei Elisa Bertino FU Haiyan | 2009 | Chinese Journal of Electronics2009，18，3： | 6 |
| 2 | Enriched endoplasmic reticulum-mitochondria interactions result in mitochondrial dysfunction and apoptosis in oocytes from obese mice显示文摘Background: Maternal obesity alters oocytes and subsequent fetal metabolism. An increasing number of studies have shown that the endoplasmic reticulums(ER) or mitochondria have important effects on oocyte quality, but there has been no study of the effect of mitochondria-associated ER membranes(MAMs) on oocyte quality. The present study was designed to assess whether the level of MAM and MAM-related proteins were different in oocytes from obese and control mice.Results: First, oocytes from mice with high-fat-diet(HFD)-induced obesity had higher levels(either greater numbers or a higher proportion for the same numbers) of MAM than oocytes from control mice. The abundance of MAM-related proteins in oocytes from obese mice was significantly greater at both the messenger RNA and protein levels, including inositol 1,4,5-trisphosphate receptor, type 1(IP3R1), inositol 1,4,5-trisphosphate receptor,type 2(IP3 R2) and phosphofurin acidic cluster sorting protein 2(PACS-2). Further, there was an increase in mitochondrial Ca^(2+)([Ca^(2+)]_m) which was associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. Down-regulation of MAM-related protein IP3R1 in oocytes from obese mice decreased [Ca^(2+)]_mand apoptosis and improved cytoplasmic maturation but did not reduce the overal MAM level. However, down-regulating MAM-related protein PACS-2 in oocytes from obese mice did reduce the level of MAM and [Ca^(2+)]_m, which decreased the rate of apoptosis and improved cytoplasmic maturation of oocytes from obese mice.Conclusions: It is possible that enriched MAM could increase [Ca^(2+)]_m, and this increase has been found to be associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. This finding suggests a novel therapeutic target for obesity-induced oocyte defects. | Lihong Zhao Tengfei Lu Lei Gao Xiangwei Fu Shien Zhu Yunpeng Hou | 2018 | Journal of Animal Science and Biotechnology2018，9，1： | 1 |
| 3 | Contextual modeling on auxiliary points for robust image reranking显示文摘Image reranking is an effective post-processing step to adjust the similarity order in image retrieval. As key components of initialized ranking lists, top-ranked neighborhoods of a given query usually play important roles in constructing dissimilarity measure. However, the number of pertinent candidates varies with respect to different queries. Thus the images with short lists of ground truth suffer from insufficient contextual information. It consequently introduces noises when using k-nearest neighbor rule to define the context. In order to alleviate this problem, this paper proposes auxiliary points which are added as assistant neighbors in an unsupervised manner. These extra points act on revealing implicit similarity in the metric space and clustering matched image pairs. By isometrically embedding each constructed metric space into the Euclidean space, the image relationships on underlying topological manifolds are locally represented by distance descriptions. Furthermore, by combining Jaccard index with our auxiliary points, we present a contextual modeling on auxiliary points ( CMAP ) method for image reranking. With richer contextual activations, the Jaccard similarity coefficient defined by local distribution achieves more reliable outputs as well as more stable parameters. Extensive experiments demonstrate the robustness and effectiveness of the proposed method. | Ying LI Xiangwei KONG Haiyan FU Qi TIAN | 2019 | Frontiers of Computer Science2019，13，5： | 1 |
| 4 | Expression and distribution of cell adhesion-related proteins in bovine parthenogenetic embryos: The effects of oocyte vitrification显示文摘 | Yan Zeng Xiangwei Fu Guangbin Zhou Mingxing Yue Yanhua Zhou Shien Zhu | 2013 | Theriogenology2013，，1： | 1 |
| 5 | Antioxidant procyanidin B2 protects oocytes against cryoinjuries via mitochondria regulated cortical tension显示文摘Background:Irreversible cryodamage caused by oocyte vitrification limited its wild application in female fertility preservation.Antioxidants were always used to antagonist the oxidative stress caused by vitrification.However,the comprehensive mechanism underlying the protective role of antioxidants has not been studied.Procyanidin B2(PCB2)is a potent natural antioxidant and its functions in response to vitrification are still unknown.In this study,the effects of PCB2 on vitrified-thawed oocytes and subsequent embryo development were explored,and the mechanisms underlying the protective role of PCB2 were systematically elucidated.Results:Vitrification induced a marked decline in oocyte quality,while PCB2 could improve oocyte viability and further development after parthenogenetic activation.A subsequent study indicated that PCB2 effectively attenuated vitrification-induced oxidative stress,rescued mitochondrial dysfunction,and improved cell viability.Moreover,PCB2 also acts as a cortical tension regulator apart from strong antioxidant properties.Increased cortical tension caused by PCB2 would maintain normal spindle morphology and promote migration,ensure correct meiosis progression and finally reduce the aneuploidy rate in vitrified oocytes.Further study reveals that ATP biosynthesis plays a crucial role in cortical tension regulation,and PCB2 effectively increased the cortical tension through the electron transfer chain pathway.Additionally,PCB2 would elevate the cortical tension in embryo cells at morula and blastocyst stages and further improve blastocyst quality.What's more,targeted metabolomics shows that PCB2 has a beneficial effect on blastocyst formation by mediating saccharides and amino acids metabolism.Conclusions:Antioxidant PCB2 exhibits multi-protective roles in response to vitrification stimuli through mitochondria-mediated cortical tension regulation. | Qingrui Zhuan Jun Li Xingzhu Du Luyao Zhang Lin Meng Yuwen Luo Dan Zhou Hongyu Liu Pengcheng Wan Yunpeng Hou Xiangwei Fu | 2023 | Journal of Animal Science and Biotechnology2023，14，1： | 0 |
| 6 | An efficient method for the sanitary vitrification of bovine oocytes in straws显示文摘Background:At present,vitrification has been widely applied to humans,mice and farm animals.To improve the efficiency of vitrification in straw,bovine oocytes were used to test a new two-step vitrification method in this study.Results:When in vitro matured oocytes were exposed to 20%ethylene glycol(EG20) for 5 min and 40%ethylene glycol(EG40) for 30 s,followed by treatment with 30%glycerol(Gly30),Gly40 or Gly50,a volume expansion was observed in Gly30 and Gly40 but not Gly50.This indicates that the intracellular osmotic pressure after a 30 s differs between EG40 and ranged between Gly40(approximately 5.6 mol/L) and Gly50(approximately 7.0 mol/L).Since oocytes are in EG40 just for only a short period of time(30 s) and at a lower temperature(4℃),we hypothesize that the main function of this step in to induce dehydration.Based on these results,we omitted the EG40 step,before oocytes were pretreated in EG20 for 5 min,exposed to pre-cooled(4℃) Gly50,for 30 s,and then dipped into liquid nitrogen.After warming,81.1%of the oocytes survived,and the surviving oocytes developed into cleavage stage embryos(63.5%) or blastocysts(20.0%) after parthenogenetic activation.Conclusions:These results demonstrate that in a two-step vitrification procedure,the permeability effect in the second step is not necessary.It is possible that the second step is only required to provide adequate osmotic pressure to condense the intracellular concentration of CPAs to a level required for successful vitrification. | Yanhua Zhou Xiangwei Fu Guangbin Zhou Baoyu Jia Yi Fang Yunpeng Hou Shien Zhu | 2014 | Journal of Animal Science and Biotechnology2014，5，4： | 0 |
| 7 | Effects of the TLR4 transgene on reproductive traits and DNA methylation pattern of oocytes in ewes显示文摘This study was conducted to systematically assess the reproductive performance of transgenic TLR4 ewes.In the TLR4 transgenic founders(F_(0))and their positive offspring(F_(1)),hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed.The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes.Moreover,the transgenic ewes showed reproductive traits similar to the wildtype ewes.These traits included characteristics of puberty,the estrus cycle,estrus duration,gestation,the pregnancy rate and the superovulation response.Additionally,no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages.In summary,the preliminary evidence presented in this paper demonstrates that the presence of the TLR4 transgene did not affect the reproductive performance in sheep. | Yi FANG Xiangwei FU Junjie LI Ming DU Baoyu JIA Jinlong ZHANG Xiaosheng ZHANG Shien ZHU | 2014 | Frontiers of Agricultural Science and Engineering2014，1，4： | 0 |
| 8 | Cryopreservation of farm animal gametes and embryos:recent updates and progress显示文摘Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation protocol has been continuously re?ned over the years to achieve greater reproductive performance; cryoprotective agents are more effective and less toxic than previously; there has been signi?cant innovation in advanced cryopreservation systems and carriers. Despite this, there are still problems that urgently require practical solutions, such as remedies for cryodamage and encouraging the use of frozen–thawed porcine sperm in pig production. | Zhengyuan HUANG Lei GAO Yunpeng HOU Shien ZHU Xiangwei FU | 2019 | Frontiers of Agricultural Science and Engineering2019，6，1： | 0 |
| 9 | DNA Methylation Pattern in Pronuclear-Stage Mouse Embryos:Effect of Oocyte Vitrification显示文摘This study was conducted to investigate the pattern of DNA methylation in pronuclear-stage mouse embryos derived from vitrified-warmed oocytes.Mouse oocytes at metaphase II(MII) stage of meiosis were allocated randomly into three groups:1) untreated(control);2) exposed to vitrification solution without being plunged into liquid nitrogen(toxicity);and 3) vitrified by open-pulled straw(OPS) method(vitrification).Oocytes were fertilized in vitro(IVF).The level of DNA methylation was examined at 8 hpf(hours post-fertilization) by immunofluorescence using an anti-5-methylcytosine(5-MeC) monoclonal antibody and fluorescein isothiocyanate(FITC)-conjugated goat anti-mouse IgG.After IVF,rates of 2-cell embryos(51.39%) and blastocysts(35.82%) for vitrified-warmed oocytes were lower(P<0.01) than that for control(70.83%,47.82%) or vitrification solution treated(64.80%,46.29%) oocytes.At 8 hpf,there were more(P<0.05) pronuclear-stage oocytes in which syngamy of pronuclei had not occurred in the vitrification group.In addition,5-MeC fluorescent intensities for the female pronucleus and zygote were lower in the vitrification group(P<0.01) compared to pronuclear-stage embryos in the control and toxicity groups.In conclusion,oocyte vitrification causes a reduction in global genomic methylation in the female pronucleus and zygote,resulting in delayed fusion of pronuclei and compromising the developmental potential of mouse zygotes and embryos. | Ying Liang Xiangwei Fu Junjie Li Dianshuai Yuan Shien Zhu | 2011 | Journal of Animal Science and Biotechnology2011，2，4： | 0 |
| 10 | PRODUCT IMAGE RETRIEVAL BASED ON CO-FEATURES OF THE OBJECT显示文摘In this paper, we propose a product image retrieval method based on the object contour corners, image texture and color. The product image mainly highlights the object and its background is very simple. According to these characteristics, we represent the object using its contour, and detect the corners of contour to reduce the number of pixels. Every corner is described using its approximate curvature based on distance. In addition, the Block Difference of Inverse Probabilities (BDIP) and Block Variation of Local Correlation (BVLC) texture features and color moment are extracted from image's HIS color space. Finally, dynamic time warping method is used to match features with different length. In order to demonstrate the effect of the proposed method, we carry out experiments in Mi-crosoft product image database, and compare it with other feature descriptors. The retrieval precision and recall curves show that our method is feasible. | Fu Haiyan Kong Xiangwei t Yang Nan Zhou Jianhui Chu Fengtao | 2010 | Journal of Electronics(China)2010，27，6： | 0 |
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