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| 1 | A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming. | Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng | 2019 | Cell Research2019，29，9： | 9 |
| 2 | Hepatic spheroids derived from human induced pluripotent stem cells in bio-artificial liver rescue porcine acute liver failure显示文摘Dear Editor,Acute liver failure(ALF)is a complicated disorder showing a nearly 80%mortality rate,and there is currently no effective medical solutions for ALF except liver transplantation.1 Bio-artificial liver(BAL)system is a device that consists of a bioreactor filled with hepatocytes,which could potentially rescue ALF patients by providing partial liver function until a suitable donor liver can be found or the native liver has self-regenerated. | Sitong Chen Jinglin Wang Haozhen Ren Yinan Liu Chengang Xiang Cheng Li Shichun Lu Yan Shi Hongkui Deng Xiaolei Shi | 2020 | Cell Research2020，30，1： | 9 |
| 3 | Associations between parental support for physical activity and moderate-to-vigorous physical activity among Chinese school children: A cross-sectional study显示文摘Purpose: The objective of this study was to examine the associations between parental support for physical activity(PA) and levels of moderate-to-vigorous physical activity(MVPA) among Chinese school children.Methods: Study participants were 81,857 school-aged children(aged 9–17 years; mean age = 13 years; 49.0% boys) and their parents or guardians who took part in the 2016 Physical Activity and Fitness in China—The Youth Study project. The independent variables included various forms of parental support for PA ascertained through a survey completed by parents or guardians. The dependent variable was the children's average MVPA in minutes per day by self-report. Structure equation modeling was used to examine the associations between parental support for PA and children's MVPA minutes.Results: The overall mean for MVPA participation time for the child participants was 48.82 ± 28.71 min/day. Significant associations between various forms of parental support and MVPA among children were found. Parental encouragement, accompanying, financial support, involvement,and role modeling for PA were positively associated with a high level of student MVPA participation. Parental PA knowledge-sharing was not associated with MVPA. The patterns of the associations were similar across sex, residence locale(i.e., urban and rural), and school grades(primary,junior middle, and junior high schools).Conclusion: Findings from this study suggest that, among Chinese school children, parental support for PA is important in promoting and facilitating children's participation in MVPA. | Yang Liu Yajun Zhang Sitong Chen Jialin Zhang Zongchen Guo Peijie Chen | 2017 | Journal of Sport and Health Science2017，6，4： | 7 |
| 4 | Start-up of the Anammox process from the conventional activated sludge in a membrane bioreactor显示文摘 | Tao Wang Hanmin Zhang Fenglin Yang Sitong Liu Zhimin Fu Huihui Chen | 2008 | Bioresource Technology2008，，9： | 2 |
| 5 | Prevalence and correlates of meeting the muscle-strengthening exercise recommendations among Chinese children and adolescents:Results from 2019 Physical Activity and Fitness in China—The Youth Study显示文摘Purpose This study aimed to describe the national prevalence of Chinese children and adolescents who met the World Health Organization muscle-strengthening exercise(MSE)recommendations and identify correlates of meeting the MSE recommendations.Methods Cross-sectional data from the 2019 Physical Activity and Fitness in China—The Youth Study,a nationally representative sample of Chinese children and adolescents(n=80,413;mean age=13.7 years;53.9%girls)and their parents,were analyzed.Children and adolescents who reported engaging≥3 days(up to 7 days)of MSE per week were classified as meeting the MSE recommendations.MSE,demographics,lifestyle behaviors(sport participation,moderate-to-vigorous physical activity,screen time,and sleep duration),exercise intention,peer and parental support,and parental MSE participation were assessed through self-reports.Logistic regression models were used to determine the correlates of meeting the MSE recommendations.The analyses were completed in 2020.Results Overall,39.3%of children and adolescents met the MSE recommendations.Girls,10th-12th graders,minorities,those from lower income households and those from families with lower parental education were less likely to meet the MSE recommendations.Children and adolescents who were proficient in≥2 sports were more likely to meet the MSE recommendations(adjusted odds ratio(aOR)=1.44,95%confidence interval(95%CI):1.26-1.65),as were those with more moderate-to-vigorous physical activity(aOR=1.57,95%CI:1.53-1.61).Additionally,children and adolescents with high exercise intention(aOR=1.60,95%CI:1.51-1.69),those whose parents met the adult MSE recommendations(aOR=1.46,95%CI:1.40-1.52),and those who received high peer(aOR=1.27,95%CI:1.20-1.34)and parental support(aOR=1.07,95%CI:1.04-1.12)were more likely to meet the MSE recommendations.Conclusion Less than two-fifths of Chinese children and adolescents met the World Health Organization MSE recommendations.The correlates identified in our study can help inform the development of school and community based strategies and policies to enhance participation in MSE and improve muscular fitness of all Chinese children and adolescents. | Fei Xin Zheng Zhu Sitong Chen Huan Chen Xiaoqing Hu Xiao Ma Kun Liang Yang Liu Lijuan Wang Yujun Cai Ang Chen Yan Tang | 2022 | Journal of Sport and Health Science2022，11，3： | 2 |
| 6 | Start-up of the Anammox process from the conventional activated sludge in a membrane bioreactor显示文摘 | Tao Wang Hanmin Zhang Fenglin Yang Sitong Liu Zhimin Fu Huihui Chen | 2008 | Bioresource Technology2008，，9： | 2 |
| 7 | Identification and functional annotation of lncRNA genes with hypermethylation in colorectal cancer显示文摘 | Qi Liao Weiling He Jianfa Liu Yi Cen Liang Luo Chengliang Yu Yang Li Sitong Chen Shiwei Duan | 2015 | Gene2015，，： | 1 |
| 8 | The development of simultaneous partial nitrification, ANAMMOX and denitrification (SNAD) process in a single reactor for nitrogen removal显示文摘 | Huihui Chen Sitong Liu Fenglin Yang Yuan Xue Tao Wang | 2008 | Bioresource Technology2008，，4： | 1 |
| 9 | Start-up of the Anammox process from the conventional activated sludge in a membrane bioreactor显示文摘 | Tao Wang Hanmin Zhang Fenglin Yang Sitong Liu Zhimin Fu Huihui Chen | 2008 | Bioresource Technology2008，，9： | 1 |
| 10 | The development of sim- uhaneous partial nitrification, ANAMMOX and denitrification(SNAD) pn)cess in a single reactor for nitrogen removal 显示文摘 | Hnihui Chen Sitong Liu Fenglin Yang | 2009 | Bioresource Tech- nology2009，100，： | 1 |
| 11 | The development of simultaneous partial nitrification,ANAMMOX and denitrification (SNAD) process in a single reactor for nitrogen removal显示文摘 | Chen Huihui Liu Sitong Yang Fenglin | 2009 | Bioresour Technol2009，100，4： | 1 |
| 12 | Growth process and short chain alcohol separation performance of fluoride-containing NaY zeolite membrane显示文摘Growth process of the NaY zeolite membranes was investigated by fluoride-containing precursor synthesis gel.Compared with the fluoride-free precursor synthesis gel,the irregular NaY zeolite crystals were dissolved into amorphous by the fluoride-containing precursor synthesis gel initially,the amorphous contained the Y-type zeolite characteristic bands by the IR characterization.The fine square NaY zeolite crystals arose from the amorphous,which were accumulated and gradually grew into a dense NaY zeolite layer on the support surface after 6.5 h.Because the excessive NaY zeolites were dissolved by the strong alkaline and fluoride-containing precursor synthesis gel,there was plenty of amorphous on NaY zeolites layer for prolonging the crystallization time.The assynthesized NaY zeolite membranes had a good separation performance and repeatability for separation of 10 wt%methanol(MeOH)/methyl methacrylate(MMA) mixture by pervaporation,the flux and separation factor were(1.27 ± 0.07) kg·M^(-2)·h^(-1) and(4900 ± 1500) at 323 K,respectively.Besides,the NaY zeolite membranes were applied to separate the other short chain alcohol from the various alcohol/organic ester and alcohol/organic ether mixtures,the NaY zeolite membranes showed high short chain alcohol perm-selectivity. | Xiaopan Chen Meihua Zhu Sitong Xiang Tian Gui TingWu Yuqin Li NaHu Izumi Kumakiri Xiangshu Chen Hidetoshi Kita | 2021 | Chinese Journal of Chemical Engineering2021，34，1： | 1 |
| 13 | The development of simuhaneous partial nitrification, ANAMMOX and denitrification (SNAD) process in a single reactor for nitrogen removal 显示文摘 | CHEN Huihui LIU Sitong YANG Fenglin | 2009 | Bioresource Technology2009，100，4： | 1 |
| 14 | Leakage detection of oil tank using terahertz spectroscopy显示文摘The necessity for safety in oil-gas storage and transportation has led to increasing technical requirements for on-line monitoring of damaged pores and oil leakage from tanks. In this study, the severity of damage of the oil tank at the micron level was detected by terahertz time-domain spectroscopy(THz-TDS), which is of great significance for the early detection and prevention of oil leakage. The THz amplitude(EP) was related to the severity of damaged according to the THz-TDS measurement of oil tanks with various degrees of damage, including intact, partially damaged, completely damaged, and seriously damaged points.Absorption and scattering effects co-occurred when the THz wave penetrated the tanks, with the collective tendencies being used to expressly monitor oil leakage from tanks. When the oil tank was damaged to form micron-level pores and the crude oil had not overflowed, the pore size was close to THz wavelength and the Mie scattering effect was obvious. After further destruction of the pores, the crude oil gradually spilled over and the scattering effect was gradually transformed from Mie scattering to Rayleigh scattering. In addition, the polar molecules in crude oil have strong resonance under the irradiation of THz wave, and the THz wave has strong attenuation. Eventually, surface tension of the oil flattens the surface of the tank, the scattering effect is gradually suppressed and replaced by the absorption effect. Absorption and scattering caused by THz waves passing through tanks coexist and have competing relationships. The change rule of EPcan successfully prove the phenomenon and can be considered as an important alternative for application to predict the degree of tank damage. Therefore, in this study, the detection of pores as small as micrometers on the oil tank was expected to greatly prevent oil leakage accidents and improve the safety of oil and gas storage and transportation. | QIN FanKai CHEN SiTong CHEN Ru ZHAN HongLei MIAO XinYang XIANG WenFeng ZHAO Kun | 2021 | Science China(Technological Sciences)2021，64，9： | 1 |
| 15 | The compact Casπ(Cas12l)‘bracelet’provides a unique structural platform for DNA manipulation显示文摘CRISPR-Cas modules serve as the adaptive nucleic acid immune systems for prokaryotes,and provide versatile tools for nucleic acid manipulation in various organisms.Here,we discovered a new miniature type V system,CRISPR-Casπ(Cas12l)(~860 aa),from the environmental metagenome.Complexed with a large guide RNA(~170 nt)comprising the tracrRNA and crRNA,Casπ(Cas12l)recognizes a unique 5′C-rich PAM for DNA cleavage under a broad range of biochemical conditions,and generates gene editing in mammalian cells.Cryo-EM study reveals a‘bracelet’architecture of Casπeffector encircling the DNA target at 3.4Åresolution,substantially different from the canonical‘two-lobe’architectures of Cas12 and Cas9 nucleases.The large guide RNA serves as a‘two-arm’scaffold for effector assembly.Our study expands the knowledge of DNA targeting mechanisms by CRISPR effectors,and offers an efficient but compact platform for DNA manipulation. | Ao Sun Cheng-Ping Li Zhihang Chen Shouyue Zhang Dan-Yuan Li Yun Yang Long-Qi Li Yuqian Zhao Kaichen Wang Zhaofu Li Jinxia Liu Sitong Liu Jia Wang Jun-Jie Gogo Liu | 2023 | Cell Research2023，33，3： | 1 |
| 16 | Application of anaerobic ammonium-oxidizing consortium to achieve completely autotrophie ammonium and sulfate removal 显示文摘 | Liu Sitong Yang Fenglin Gong Zheng Meng Fangang Chen Huihui Xue Yuan Kenji Furukawa | 2008 | Bioresource Technology2008，99，10： | 1 |
| 17 | The effect of continuous Jue tone intervention on blood pressure and vasoactive substances in hypertensive rats with a liver-fire hyperactivity pattern显示文摘Objective:Jue tone is a kind of sound,using 3-mi as the main tone,and is melodious,profound,makes people feel comfortable and pleasant.This study aimed to explore the probable mechanism of Jue tone to reduce blood pressure(BP)in hypertensive rats with a liver-fire hyperactivity pattern by observing changes in BP as well as physiochemical indexes in plasma.Methods:Sixteen male spontaneous hypertensive rats(SHR)with a liver-fire hyperactivity pattern were randomly divided into a control group and a Jue tone group.The rats in the Jue tone group were treated with Jue tone(55e65 dB,played by the five elements of music rhythm instrument,a kind of physiotherapy regimen music played by Shen Wu)once a day for 4 weeks.The BP levels in each group were measured twice a week,on Monday and Friday.The levels of angiotensin II(Ang-II),thromboxane B2(TXB2),endothelin-1(ET-1),calcitonin gene-related peptide(cGRP),norepinephrine(NE),cortisol(CORT),and 5-hydroxytryptamine(5-HT).in plasma were detected by enzyme-linked immunosorbent assay after 4 weeks of intervention.Results:BP and the levels of TXB2 and ET-1 in the plasma of the treatment group were significantly lower than those of the control group,while the level of cGRP was significantly higher.Conclusion:Jue tone can reduce the BP of hypertensive rats with a liver-fire hyperactivity pattern.The mechanism may correlate with a reduction in TXB2 and ET-1 and an increase in cGRP with the reduction of reactive oxygen species(ROS). | Qian Zhou Yufeng Chen Sitong Zhang Pei Yang Tian Wang Jianxin Chen Huihui Zhao Wei Wang Xueling Ma | 2022 | Journal of Traditional Chinese Medical Sciences2022，9，1： | 0 |
| 18 | Identification of Expression Pattern and Clinical Significance of the Small Cajal Body-specific RNA SCARNA16 in Hepatocellular Carcinoma显示文摘Background and Aims:For high morbidity and mortality,hepatocellular carcinoma(HCC)becomes a major health issue worldwide.Nowadays,numerous non-coding RNAs(ncRNAs)are known to regulate the occurrence and patho-genesis of tumors.Some ncRNAs have also been developed as tumor biomarkers and therapeutic targets.However,the potential function of the small Cajal body-specific RNA(scaRNA)SCARNA16,a newly identified ncRNA,remains to be explored in HCC.Methods:In both HCC cell lines and specimens from 120 enrolled patients,the expression val-ues of SCARNA16 were detected.We divided patients into SCARNA16 high and low expression subgroups,and then analyzed the difference of various clinical characteristics and prognosis data between subgroups.Results:Compared to paired controls,SCARNA16 was significantly down-regulated in HCC cell lines and clinical specimens(p<0.01).Besides,HCC patients with lower SCARNA16 expression commonly presented with larger and more tumor lesions,more ves-sel carcinoma emboli,more capsular invasion and higher TNM stages(p<0.05).Moreover,SCARNA16 expression was negatively correlated with postoperative prognosis of HCC patients in 5-year follow-up,including tumor-free survival(TFS)(median time of low vs.high subgroups:14 vs.48 months,p=0.006)and overall survival(OS)(median time of low vs.high subgroups:39 vs.52 months,p=0.001).Besides,SCARNA16 acted as an independent prognostic bio-marker in TFS(hazard ratio[HR]:0.578,95%CI:0.345-0.969,p=0.038)and OS(HR:0.366,95%CI:0.178-0.752,p=0.006).Conclusions:Low expression patterns of SCAR-NA16 remarkably associated with severe clinical status and poor survival of patients,suggesting that SCARNA16 pos-sesses potency as a novel biomarker for HCC. | Sitong Zhang Yuan Ding Zhongquan Sun Yao Ge Yanjie Li Xin Han Qianhui Xu Liuzhi Zhou Yining Chen Hao Xu Yang Bai Chang Xu Hao Ding Sheng Yan Weilin Wang | 2022 | Journal of Clinical and Translational Hepatology2022，10，1： | 0 |
| 19 | Move More,Sit Less and Sleep Well:An analysis of WHO movement guidelines for children under 5 years of age显示文摘Insufficient physical activity(PA),prolonged sedentary behavior(SB)and inadequate sleep(SLP)are detrimental factors to population health.To address health issues caused by insufficient PA,excessive SB and poor SLP,the World Health Organization(WHO)updated PA and SB guidelines for all populations aged from 5 years to 65 years and older in 2020.For children under 5 years old,the WHO issued the first global guidelines for PA,SB and SLP(collectively refer to movement behaviors)in April 2019.The guidelines applied a holistic approach to promote health behaviors,filling the gap of no comprehensive global movement guidelines for young children.Although the guidelines for young children offer guidance for health promotion,some research and practice implications and other issues should be mentioned.This commentary includes considerations for the importance of the WHO guidelines for younger children,critical analysis of evidence for developing the guidelines,and recommendations for future research and practice.The aim of this paper is to further advance health research in younger populations. | Sitong Chen Jintao Hong Guangxu Wang Yang Liu | 2021 | Sports Medicine and Health Science2021，3，1： | 0 |
| 20 | Open-source dataset for control-oriented modelling in diesel engines显示文摘In this study, an experimental dataset of a diesel engine under the European transient cycle (ETC)is demonstrated and opened for academic research.The dataset with 7 dimensional features is collected from the advanced diesel engine manufactured by Changchun FAW Sihuan Engine Manufacture Co., Ltd. | Jinghua ZHAO Sitong ZHOU Yunfeng HU Mingjun JU Ruixue REN Hong CHEN | 2019 | Science China(Information Sciences)2019，62，7： | 0 |
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