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| 1 | An acorn squash (Cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in Cucurbita显示文摘Acorn squash(Cucurbita pepo)is an iconic fall vegetable in the United States,known for its unique fruit shape and also prized for its culinary properties.Little is known about the metabolism that underlies the development of fruit quality attributes such as color,sweetness,texture and nutritional qualities in acorn squash,or any other winter squash grown worldwide.To provide insight into winter squash fruit and seed development and add to the genomic resources in the Cucurbita genus,RNA sequencing was used to generate an acorn squash fruit and seed transcriptome from the cultivar Sweet REBA at critical points throughout fruit development.141838600 high-quality paired-end Illumina reads were assembled into 55949 unigenes.85%of unigenes with predicted open reading frames had homology with previously identified genes and over 62%could be functionally annotated.Comparison with the watermelon and cucumber genomes provided confirmation that the unigenes are full-length and comprehensive,covering an average of 90%of the coding sequence of their homologs and 72%of the cucumber and watermelon exomes.Key candidate genes associated with carotenoid and carbohydrate metabolism were identified toward a resource for winter squash fruit quality trait dissection.This transcriptome represents a major advance in C.pepo genomics,providing significant new sequence information and revealing the repertoire of genes expressed throughout winter squash fruit and seed development.Future studies on the genetic basis of fruit quality and future breeding efforts will be enhanced by tools and insights developed from this resource. | Lindsay E Wyatt Susan R Strickler Lukas A Mueller Michael Mazourek | 2015 | Horticulture Research2015，2，1： | 11 |
| 2 | Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin显示文摘Both the fruit mesocarp and the seeds of winter squash can be used for consumption,although the focus of breeding efforts varies by cultivar.Cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content,percent dry matter,and percent soluble solids,while these traits are essentially ignored in oilseed pumpkins.To compare fruit development in these two types of squash,we sequenced the fruit transcriptome of two cultivars bred for different purposes:an acorn squash,‘Sweet REBA’,and an oilseed pumpkin,‘Lady Godiva’.Putative metabolic pathways were developed for carotenoid,starch,and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the pathways.Gene expression,especially of known rate-limiting and branch point genes,corresponded with metabolite accumulation both across development and between the two cultivars.Thus,developmental regulation of metabolite genes is an important factor in winter squash fruit quality. | Lindsay E Wyatt Susan R Strickler Lukas A Mueller Michael Mazourek | 2016 | Horticulture Research2016，3，1： | 7 |
| 3 | Adipokines Linking Obesity with Colorectal Cancer Risk in Postmenopausal Women显示文摘 | Gloria Y.F. Ho Tao Wang Marc J. Gunter Howard D. Strickler Mary Cushman Robert C. Kaplan Sylvia Wassertheil-Smoller Xiaonan Xue Swapnil N. Rajpathak Rowan T. Chlebowski Mara Z. Vitolins Philipp E. Scherer Thomas E. Rohan | 2012 | Cancer Research2012，，12： | 2 |
| 4 | Splenic vascular tumors:a histologic,immunophenotypic,and virologic study显示文摘 | Arber DA Strickler JG Chen YY | 1997 | Am J Surg Pathol1997，21，7： | 1 |
| 5 | Rapid purification of staphylococcal enterotoxin B by high-pressure liquid chromatography显示文摘 | Strickler M P Neill R J Stone M J | 1989 | J Clin Microbiol1989，27，5： | 1 |
| 6 | Splenic vascular tumour:A histologic,immunohenotypic and virologic study显示文摘 | Arber DA Strickler JG Chen YY | | 0，，： | 1 |
| 7 | Simulated Climatology of a General Circulation Model with a Hydrologic Cycle 显示文摘 | MANABE S SMAGORINSKY J STRICKLER R F | 1965 | Monthly Weather Review1965，，93： | 1 |
| 8 | Two-photon Laser Scanning Fluorescence Microscopy 显示文摘 | DENK W STRICKLER J H | 1990 | Science1990，248，1： | 1 |
| 9 | Insulin-like growth factors and coronary heart disease显示文摘 | Kaplan RC Strickler HD Rohan TE | 2005 | Cardiol Rev2005，13，1： | 1 |
| 10 | Three-dimensional optical data storage in refractive media by two-photon point excitation 显示文摘 | STRICKLER J H WEBB W W | 1991 | Opt Lett1991，16，： | 1 |
| 11 | Two-photon laser scanning fluorescence microscopy显示文摘 | Denk W Strickler J H Webb W W | 1990 | Science1990，248，： | 1 |
| 12 | lrlomeostatic proliferation plus regulatory T cell depletion promotes potent rejection of B16 melanoma显示文摘 | Kline J Brown IE Zha YY Blank C Strickler J Wouters H Zhang L Gajewski TF | 2008 | Clin Cancer Res2008，14，： | 1 |
| 13 | Natural history and possiblereactivationofhumanpapillomavirusinhuman immunodeficiency virus-positive women显示文摘 | Strickler HD Burk RD Fazzari M | | 0，，： | 1 |
| 14 | Simulated climatology of a general circulation model with a hydrological cycle显示文摘 | MANABE S SMAGORINSKY J STRICKLER R F | 1965 | Mon Wea Rev1965，93，12： | 1 |
| 15 | Experiment Extended Prediction with a Nine Level Hem ispheric Model 显示文摘 | MIYAKODAK SMAGORINSKY J STRICKLER R F | 1969 | Monthly Weather Review1969，，97： | 1 |
| 16 | Two - photon laser scanning fluorescence microscopy显示文摘 | Denk W Strickler JH Webb WW | 1990 | Science1990，248，： | 1 |
| 17 | Two-photon laser scanning fluorescence microscopy显示文摘 | Denk W Strickler JH Webb WW | 1990 | Science1990，248，4951： | 1 |
| 18 | Twophoton laser scanning fluorescence microcopy显示文摘 | DENK W F JAMES H STRICKLER | 1990 | Science1990，248，： | 1 |
| 19 | Insulin-like growtts factors and coronary heart disease 显示文摘 | Kaplan RC Strickler kiD Rohan TE | 2005 | Cardiol Rev2005，13，1： | 1 |
| 20 | Splenic vascular tumors:a histologic,immunophenotypic,and virologic study显示文摘 | Arber DA Strickler JG Chen YY | 1997 | Am J Surg Pathol1997，21，7： | 1 |
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