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| 1 | Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study显示文摘A simple and rapid liquid chromatography-tandem mass spectrometric(LC-MS/MS) assay method has been developed and fully validated for simultaneous quantification of pioglitazone and candesartan in human plasma.Irbesartan was used as an internal standard.The analytes were extracted from human plasma samples by solid-phase extraction technique using a Strata-X 33 mm polymeric sorbent.The reconstituted samples were chromatographed on a C18 column by using a 80:20(v/v) mixture of acetonitrile and 0.1% formic acid as the mobile phase at a flow rate of 0.8 mL/min.The calibration curves obtained were linear(rZ0.99) over the concentration range of 15-3000 ng/mL for pioglitazone and 5-608 ng/mL for candesartan.The results of the intra-and inter-day precision and accuracy studies were well within the acceptable limits.A run time of 2.7 min for each sample made it possible to analyze more than 300 plasma samples per day.The proposed method was found to be applicable to clinical studies. | Vijaya Kumari Karra Nageswara Rao Pilli Jaswanth Kumar Inamadugu J.V.L.N.Seshagiri Rao | 2012 | Journal of Pharmaceutical Analysis2012，2，3： | 8 |
| 2 | Simultaneous determination of telmisartan and amlodipine in human plasma by LC-MS/MS and its application in a human pharmacokinetic study显示文摘A rapid and sensitive liquid chromatography-tandem mass spectrometric(LC-MS/MS) assay method has been developed and fully validated for the simultaneous quantification of telmisartan and amlodipine in human plasma.Carbamazepine was used as an internal standard.Analytes and the internal standard were extracted from human plasma by solid-phase extraction technique using Waters Oasis HLB 1 cm 3(30 mg) extraction cartridge.The reconstituted samples were chromatographed on a Hypurity advance C 18 column(50 mm×4.6 mm,5 μm) using a mixture of acetonitrile-5 mM ammonium acetate buffer(pH-4.0)(50:50,v/v) as the mobile phase at a flow rate of 0.8 mL/min.The calibration curve obtained was linear(r≥0.99) over the concentration range of 2.01-400.06 ng/mL for telmisartan and 0.05-10.01 ng/mL for amlodipine.Method validation was performed as per FDA guidelines and the results met the acceptance criteria.A run time of 2.5 min for each sample made it possible to analyze more than 400 human plasma samples per day.The proposed method was found to be applicable to clinical studies. | Vasu Babu Ravi Jaswanth Kumar Inamadugu Nageswara Rao Pilli Vudagandla Sreenivasulu Venkateswarlu Ponneri | 2012 | Journal of Pharmaceutical Analysis2012，2，5： | 8 |
| 3 | High performance liquid chromatography mass spectrometric method for the simultaneous quantification of pravastatin and aspirin in human plasma:Pharmacokinetic application显示文摘A rapid and sensitive liquid chromatography-tandem mass spectrometric(LC-MS/MS) assay method has been developed and fully validated for the simultaneous quantification of pravastatin and aspirin in human plasma.Furosemide was used as an internal standard.Analytes and the internal standard were extracted from human plasma by liquid-liquid extraction technique using methyl tertiary butyl ether.The reconstituted samples were chromatographed on a Zorbax SB-C_18 column by using a mixture of 5 mM ammonium acetate buffer and acetonitrile(20:80,v/v) as the mobile phase at a flow rate of 0.8 mL/min.The calibration curve obtained was linear(r≥0.99) over the concentration range of 0.50-600.29 ng/mL for pravastatin and 20.07-2012.00 ng/mL for aspirin.Method validation was performed as per FDA guidelines and the results met the acceptance criteria.A run time of 2.0 min for each sample made it possible to analyze more than 400 human plasma samples per day.The proposed method was found to be applicable to clinical studies. | Srinivasa Rao Polagani Nageswara Rao Pilli Venkateswarlu Gandu | 2012 | Journal of Pharmaceutical Analysis2012，2，3： | 5 |
| 4 | Simultaneous determination of atorvastatin,metformin and glimepiride in human plasma by LC-MS/MS and its application to a human pharmacokinetic study显示文摘A simple, rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/ MS) assay method has been developed and fully validated for the simultaneous quantification of atorvastatin, metformin and glimepiride in human plasma. Carbamazepine was used as internal standard (IS). The analytes were extracted from 200 mL aliquots of human plasma via protein precipitation using acetonitrile. The reconstituted samples were chromatographed on a Alltima HP C18 column by using a 60:40 (v/v) mixture of acetonitrile and 10 mM ammonium acetate (pH 3.0) as the mobile phase at a flow rate of 1.1 mL/min. The calibration curves obtained were linear (r 2 Z0.99) over the concentration range of 0.50-150.03 ng/mL for atorvastatin, 12.14-1207.50 ng/mL for metformin and 4.98-494.29 ng/mL for glimepiride. The API-4000 LC-MS/MS in multiple reaction monitoring (MRM) mode was used for detection. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. All the analytes were found to be stable in a battery of stability studies. The method is precise and sensitive enough for its intended purpose. A run time of 2.5 min for each sample made it possible to analyze more than 300 plasma samples per day. The developed assay method was successfully applied to a pharmacokinetic study in human male volunteers. | Srinivasa Rao Polagani Nageswara Rao Pilli Ramakrishna Gajula Venkateswarlu Gandu | 2013 | Journal of Pharmaceutical Analysis2013，3，1： | 3 |
| 5 | A rapid and sensitive liquid chromatography-tandem mass spectrometric assay for duloxetine in human plasma:Its pharmacokinetic application显示文摘This paper describes a simple, rapid and sensitive liquid chromatography-tandem mass spectrometry assay for the determination of duloxetine in human plasma. A duloxetine stable labeled isotope (duloxetine d 5 ) was used as an internal standard. Analyte and the internal standard were extracted from 100 mL of human plasma via solid phase extraction technique using Oasis HLB cartridges. The chromatographic separation was achieved on a C 18 column by using a mixture of acetonitrile-5 mM ammonium acetate buffer (83:17, v/v) as the mobile phase at a flow rate of 0.9 mL/min. The calibration curve obtained was linear (r 2 Z0.99) over the concentration range of 0.05-101 ng/mL. Multiple-reaction monitoring mode (MRM) was used for quantification of ion transitions at m/z 298.3/154.1 and 303.3/159.1 for the drug and the internal standard, respectively. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies. | Ramakrishna Gajula Rambabu Maddela Vasu Babu Ravi Jaswanth Kumar Inamadugu Nageswara Rao Pilli | 2013 | Journal of Pharmaceutical Analysis2013，3，1： | 3 |
| 6 | The influence of personality traits and persuasive messages on entrepreneurial intention: A cross-cultural comparison显示文摘 | Emmeline de Pillis Kathleen K. Reardon | 2007 | Career Development International2007，，4： | 2 |
| 7 | Ultrasonic pretreatment of sludge: A review显示文摘 | Sridhar Pilli Puspendu Bhunia Song Yan R.J. LeBlanc R.D. Tyagi R.Y. Surampalli | 2010 | Ultrasonics - Sonochemistry2010，，1： | 2 |
| 8 | Effects of operating conditions on oil loss and properties of products obtained by co-rotating twin-screw extrusion of fatty meal: preliminary study显示文摘 | Teresa De Pilli Carla Severin Antonietta Baiano | 2005 | Journal of Food Engineering2005，70，1： | 1 |
| 9 | Towards a functional and simplified allometry for estimating forest biomass 显示文摘 | Pilli R Anfodillo T Carrer M | 2006 | Forest Ecology and Management2006，237，13： | 1 |
| 10 | Application of vacuum impregnation techniques to improve the pH reduction of vegetables : study on carrots and eggplants 显示文摘 | Derossi A Pilli T D Severini C | 2013 | Food and Bioprocess Technology2013，6，11： | 1 |
| 11 | Radiofrequeucy ther- mal ablation for hepatoceIlular carcinoma stimulates autologous NK-cell response显示文摘 | Zerbini A Pilli M Laccabue D | 2010 | Gastroenterology2010，27，1： | 1 |
| 12 | Getting heavier,younger:trajectories of obesity over the life course显示文摘 | Lee JM Pilli S Gebremariam A | 2010 | Int J Obes2010，34，4： | 1 |
| 13 | A rapid and sensitive LC-MS/MS method for quantification of donepezil and its active metabolite,6-O-desmethyl donepezil in human plasma and its pharmacokinetic application显示文摘 | PILLI NR INAMADUGU JK KONDREDDY N | 2011 | Biomed Chromatogr2011，25，8： | 1 |
| 14 | MADD knock-down enhances dox- orubicin and TRAIL induced apoptosis in breast cancer cells 显示文摘 | Turner A Li LC Pilli T | 2013 | Plos one2013，8，56： | 1 |
| 15 | Lamivudine treatment can overcome cytotoxic T-cell hyporesponsiveness in chronic hepatitis B: New perspectives for immune therapy显示文摘 | Carolina Boni Amalia Penna Graham S. Ogg Antonio Bertoletti Massimo Pilli Cristina Cavallo Albertina Cavalli Simona Urbani Richard Boehme Ruggero Panebianco Franco Fiaccadori Carlo Ferrari | 2001 | Hepatology2001，，4： | 1 |
| 16 | The stemona alkaloids显示文摘 | Pilli R A Rosso G B De Oliveira Mda C | 2005 | Alkaloids Chem Biol2005，62，30： | 1 |
| 17 | Model updating by adding known masses 显示文摘 | Cha P D L G de Pillis | 2001 | International Journal for Numerical Method in Engineering2001，50，： | 1 |
| 18 | Fourier-domain optical coherence tomography of eyes with idiopathic epiretinal membrane: correlation between macular morphology and visual function显示文摘 | Pilli S Lim P Zawadzki RJ Choi SS WernerJS Park SS | 2011 | Eye ( Lond)2011，25，6： | 1 |
| 19 | Cobalt-Phosphate ( Co- Pi) Catalyst Modified Mo-Doped BiVO4 Photoeleetrodes for Solar Water Oxidation 显示文摘 | Pilli S K Furtak T E Brown L D | 2011 | Energy & Environmental Science2011，，4： | 1 |
| 20 | Prevention of enzymatic browning in sliced potatoes by blanching in boiling saline solutions显示文摘 | C Severini A Baiano T De Pilli | 2003 | LWT-Food Science and Technology2003，36，7： | 1 |
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