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| 1 | Kinsenoside attenuates osteoarthritis by repolarizing macrophages through inactivating NF-κB/MAPK signaling and protecting chondrocytes显示文摘The objective was to investigate the effect of kinsenoside(Kin) treatments on macrophage polarity and evaluate the resulting protection of chondrocytes to attenuate osteoarthritis(OA) progression.RAW264.7 macrophages were polarized to M1/M2 subtypes then administered with different concentrations of Kin. The polarization transitions were evaluated with quantitative real-time polymerase chain reaction(q RT-PCR), confocal observation and flow cytometry analysis. The mechanism of Kin repolarizing M1 macrophages was evaluated by Western blot. Further, macrophage conditioned medium(CM) and IL-1β were administered to chondrocytes. Micro-CT scanning and histological observations were conducted in vivo on anterior cruciate ligament transection(ACLT) mice with or without Kin treatment. We found that Kin repolarized M1 macrophages to the M2 phenotype. Mechanistically, Kin inhibited the phosphorylation of IκBα, which further reduced the downstream phosphorylation of P65 in nuclear factor-κB(NF-κB) signaling. Moreover, Kin inhibited mitogen-activated protein kinases(MAPK) signaling molecules p-JNK, p-ERK and p-P38. Additionally, Kin attenuated macrophage CM and IL-1β-induced chondrocyte damage. In vivo, Kin reduced the infiltration of M1 macrophages,promoted M2 macrophages in the synovium, inhibited subchondral bone destruction and reduced articular cartilage damage induced by ACLT. All the results indicated that Kin is an effective therapeutic candidate for OA treatment. | Feng Zhou Jingtian Mei Xiuguo Han Hanjun Li Shengbing Yang Minqi Wang Linyang Chu Han Qiao Tingting Tang | 2019 | Acta Pharmaceutica Sinica B2019，9，5： | 35 |
| 2 | Targeting ferroptosis suppresses osteocyte glucolipotoxicity and alleviates diabetic osteoporosis显示文摘Diabetic osteoporosis(DOP) is the leading complication continuously threatening the bone health of patients with diabetes. A key pathogenic factor in DOP is loss of osteocyte viability. However, the mechanism of osteocyte death remains unclear. Here, we identified ferroptosis, which is iron-dependent programmed cell death, as a critical mechanism of osteocyte death in murine models of DOP. The diabetic microenvironment significantly enhanced osteocyte ferroptosis in vitro, as shown by the substantial lipid peroxidation, iron overload, and aberrant activation of the ferroptosis pathway. RNA sequencing showed that heme oxygenase-1(HO-1) expression was notably upregulated in ferroptotic osteocytes. Further findings revealed that HO-1 was essential for osteocyte ferroptosis in DOP and that its promoter activity was controlled by the interaction between the upstream NRF2 and c-JUN transcription factors. Targeting ferroptosis or HO-1 efficiently rescued osteocyte death in DOP by disrupting the vicious cycle between lipid peroxidation and HO-1 activation, eventually ameliorating trabecular deterioration. Our study provides insight into DOP pathogenesis, and our results provide a mechanism-based strategy for clinical DOP treatment. | Yiqi Yang Yixuan Lin Minqi Wang Kai Yuan Qishan Wang Pei Mu Jingke Du Zhifeng Yu Shengbing Yang Kai Huang Yugang Wang Hanjun Li Tingting Tang | 2022 | Bone Research2022，10，3： | 19 |
| 3 | QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples显示文摘Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data'. | Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu Xiaoduo Lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li | 2019 | Molecular Plant2019，12，3： | 13 |
| 4 | Zinc alloy-based bone internal fixation screw with antibacterial and anti-osteolytic properties显示文摘There is no targeted effective treatment for patients undergoing internal fixation surgery/two-stage total joint revision surgery with a high risk of postoperative infection and osteolysis,while the rate of reoperation due to infection and osteolysis remains high.In this study,we report a pioneering application of implants made of biodegradable Zn-Ag alloy with active antibacterial and anti-osteolytic properties in three classical animal models,illustrating antibacterial,anti-osteolysis,and internal fixation for fractures.The antibacterial activity of the Zn-2Ag alloy was verified in a rat femur osteomyelitis prevention model,while the anti-osteolytic properties were evaluated using a mouse cranial osteolysis model.Moreover,the Zn-2Ag based screws showed similar performance in bone fracture fixation compared to the Ti-6Al-4V counterpart.The fracture healed completely after 3 months in the rabbit femoral condyle fracture model.Furthermore,the underlying antibacterial mechanism may include inhibition of biofilm formation,autolysis-related pathways,and antibiotic resistance pathways.Osseointegration mechanisms may include inhibition of osteoclast-associated protein expression,no effect on osteogenic protein expression,and no activation of related inflammatory protein expression.The empirical findings here reveal the great potential of Zn-Ag-based alloys for degradable biomaterials in internal fixation surgery/two-stage total joint revision surgery for patients with a high risk of postoperative infection and osteolysis. | Xinhua Qu Hongtao Yang Bo Jia Minqi Wang Bing Yue Yufeng Zheng Kerong Dai | 2021 | Bioactive Materials2021，6，12： | 4 |
| 5 | A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection显示文摘Large bone defects face a high risk of pathogen exposure due to open wounds,which leads to high infection rates and delayed bone union.To promote successful repair of infectious bone defects,fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required.This study describes creation of an engineered progenitor cell line(C3H10T1/2)capable of doxycycline(DOX)-mediated release of bone morphogenetic protein-2(BMP2).Three-dimensional bioprinting technology enabled creation of scaffolds,comprising polycaprolactone/mesoporous bioactive glass/DOX and bioink,containing these engineered cells.In vivo and in vitro experiments confirmed that the scaffold could actively secrete BMP2 to significantly promote osteoblast differentiation and induce ectopic bone formation.Additionally,the scaffold exhibited broad-spectrum antibacterial capacity,thereby ensuring the survival of embedded engineered cells when facing high risk of infection.These findings demonstrated the efficacy of this bioprinted scaffold to release BMP2 in a controlled manner and prevent the occurrence of infection;thus,showing its potential for repairing infectious bone defects. | Minqi Wang Hanjun Li Yiqi Yang Kai Yuan Feng Zhou Haibei Liu Qinghui Zhou Shengbing Yang Tingting Tang | 2021 | Bioactive Materials2021，6，5： | 3 |
| 6 | 近年中国北方草地变绿受降水增加的驱动显示文摘中国北方的暖湿化是近期的热点话题,然而其对植被生长的影响仍不清楚。本研究基于长时间序列(1982–2018年)数据,研究了归一化植被指数(NDVI)的时间动态及其气候驱动因子之间的关系,以探索近年来气候的暖湿化是否会导致该区植被变绿。我们采用分段回归探测了NDVI的变化趋势是否存在转变点,用Pearson相关分析描述了植被指数与气候因子的关系。最后,采用逐步多元回归方法研究了气候因子对NDVI时间变化的贡献率。研究结果表明,NDVI时间变化趋势的转变点出现在2008年,GIMMS NDVI在1982–2008年略有增加,上升速率为0.00022 yr?1,在2008–2015年上升速率达到0.002 yr?1,MODIS NDVI在2008–2018年上升速率为0.0018 yr?1。降水是NDVI变化的主要驱动因子,气温和饱和水汽压差(VPD)对NDVI的变化影响较小。总体看来,NDVI时间序列变化趋势存在转变点,并且近期气候的暖湿化主导了中国北方草地植被变绿,这为今后更好地预测该地区气候变化下的植被覆盖变化提供了依据。 | Kai Di Zhongmin Hu Mei Wang Ruochen Cao Minqi Liang Genan Wu Ruru Chen Guangcun Hao Yaolong Zhao | 2021 | Journal of Plant Ecology2021，14，5： | 2 |
| 7 | The effects of nano-SiO 2 additive on the zinc phosphating of carbon steel显示文摘 | Minqi Sheng Yi Wang Qingdong Zhong Hongyan Wu Qiongyu Zhou Hai Lin | 2010 | Surface & Coatings Technology2010，，11： | 1 |
| 8 | MicroRNA-505 identified from patients with essential hypertension impairs endothelial cell migration and tube formation显示文摘 | Qinbo Yang Chenglin Jia Peiwei Wang Minqi Xiong Jingang Cui Li Li Wenjian Wang Qingyu Wu Yu Chen Teng Zhang | 2014 | International Journal of Cardiology2014，，： | 1 |
| 9 | Carbon nanofibers membrane bridged with graphene nanosheet and hyperbranched polymer for high-performance osmotic energy harvesting显示文摘Reverse electrodialysis(RED),based on ion-selective membranes,is one of the most promising technologies for capturing osmotic energy.As key elements of the RED system,ion-selective membranes must meet the crucial demands of mechanical stability,anti-fouling characteristics,easy fabrication,and high power density;however,this still remains a challenge.In this study,we demonstrated a large-scale,mechanically stable,and high-porosity membrane obtained by combining carbon nanomaterials and hyperbranched polyethyleneimine(h-PEI),thereby achieving a high power density of 5.0 W·m−2 with seawater and river water.Carbon nanofibers(CNFs)were subsequently bridged with graphene and h-PEI to strengthen the interaction between the CNFs,reduce the channel size and increase the space charge density,mechanical strength,and toughness.The large-scale and mechanically stable membrane fabricated using the modified CNFs exhibited anion selectivity and high ionic conductivity,thereby achieving a high-performance osmotic energy conversion.Furthermore,the anti-fouling property of the membrane was confirmed by the stability of the osmotic energy conversion in a solution with algae,which can be attributed to the high porosity of carbon nanomaterials.This economic and convenient method for the ion-selective membrane preparation is believed to be promising for large-scale osmotic energy harvesting. | Weijie Wang Jinlin Hao Qian Sun Minqi Zhao Huiyi Liu Chao Li Xin Sui | 2023 | Nano Research2023，16，1： | 1 |
| 10 | Cytotoxic activities of chitosan nanoparticles and copper-loaded nanoparticles显示文摘 | Lifeng Qi Zirong Xu Xia Jiang Yan Li Minqi Wang | 2005 | Bioorganic & Medicinal Chemistry Letters2005，，5： | 1 |
| 11 | A new benzaldehyde from the coral-derived fungus Aspergillus terreus C23-3 and its anti-inflammatory effects via suppression of MAPK signaling pathway in RAW264.7 cells显示文摘Marine fungi are important members of the marine microbiome,which have been paid growing attention by scientists in recent years.The secondary metabolites of marine fungi have been reported to contain rich and diverse compounds with novel structures(Chen et al.,2019).Aspergillus terreus,the higher level marine fungus of the Aspergillus genus(family of Trichocomaceae,order of Eurotiales,class of Euro・tiomycetes,phylum of Ascomycota). | Minqi CHEN Jinyue LIANG Yuan WANG Yayue LIU Chunxia ZHOU Pengzhi HONG Yi ZHANG Zhong-Ji QIAN | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，3： | 1 |
| 12 | Efficacy of the components of a behavioral safety program 显示文摘 | Ray Paul S Bishop Philip A Wang Minqi | 1997 | International Journal of Industrial Ergonomics1997，19，1： | 1 |
| 13 | Felodipine enhances aminoglycosides efficacy against implant infections caused by methicillin-resistant Staphylococcus aureus,persisters and biofilms显示文摘Methicillin-resistant Staphylococcus aureus(MRSA),biofilms,and persisters are three major factors leading to recurrent and recalcitrant implant infections.Although antibiotics are still the primary treatment for chronic implant infections in clinical,only few drugs are effective in clearing persisters and formed biofilms.Here,felodipine,a dihydropyridine calcium channel blocker,was reported for the first time to have antibacterial effects against MRSA,biofilm,and persisters.Even after continuous exposure to sub-lethal concentrations of felodipine,bacteria are less likely to develop resistance.Besides,low doses of felodipine enhances the antibacterial activity of gentamicin by inhibiting the expression of protein associated with aminoglycoside resistance(aacA-aphD).Next,biofilm eradication test and persisters killing assay suggested felodipine has an excellent bactericidal effect against formed biofilms and persisters.Furthermore,the result of protein profiling,and quantitative metabonomics analysis indicated felodipine reduce MRSA virulence(agrABC),biofilm formation and TCA cycle.Then,molecular docking showed felodipine inhibit the growth of persisters by binding to the H pocket of ClpP protease,which could lead to substantial protein degradation.Furthermore,murine infection models suggested felodipine in combination with gentamicin alleviate bacterial burden and inflammatory response.In conclusion,low dose of felodipine might be a promising agent for biomaterial delivery to enhance aminoglycosides efficacy against implant infections caused by MRSA,biofilm,and persisters. | Shutao Zhang Xinhua Qu Juyang Jiao Haozheng Tang Minqi Wang You Wang Hongtao Yang Weien Yuan Bing Yue | 2022 | Bioactive Materials2022，7，8： | 0 |
| 14 | Physical modeling of long-term dynamic characteristics of the subgrade for medium-low-speed maglevs显示文摘To investigate the dynamic characteristics and long-term dynamic stability of the new subgrade structure of medium-low-speed(MLS)maglevs,cyclic vibration tests were performed under natural and rainfall conditions,and the dynamic response of the subgrade structure was monitored.The dynamic response attenuation characteristics along the depth direction of the subgrade were compared,and the distribution characteristics of the dynamic stress on the surface of the subgrade along the longitudinal direction of the line were analyzed.The critical dynamic stress and cumulative deformation were used as indicators to evaluate the long-term dynamic stability of the subgrade.Results show that water has a certain effect on the dynamic characteristics of the subgrade,and the dynamic stress and acceleration increase with the water content.With the dowel steel structure set between the rail-bearing beams,stress concentration at the end of the loaded beam can be prevented,and the diffusion distance of the dynamic stress along the longitudinal direction increases.The dynamic stress measured in the subgrade bed range is less than 1/5 of the critical dynamic stress.The postconstruction settlement of the subgrade after similarity ratio conversion is 3.94 mm and 7.72 mm under natural and rainfall conditions,respectively,and both values are less than the 30 mm limit,indicating that the MLS maglev subgrade structure has good long-term dynamic stability. | Minqi Dong Wubin Wang Chengjin Wang Zhichao Huang Zhaofeng Ding Zhixing Deng Qian Su | 2023 | Railway Engineering Science2023，31，3： | 0 |
| 15 | Host-Defense-Peptide-Mimickingβ-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously显示文摘Host defense peptides(HDPs)are one of the potentially promising agents for infection diseases due to their broad spectrum and low resistance rate,but their clinical applications are limited by proteolytic instability,high-cost,and complicated synthesis process.Here,we report a host-defense-peptidemimickingβ-peptide polymer that resists proteolysis to have enhanced the activity under physiological conditions,excellent antimicrobial efficiency even at high density of bacteria,and low cost for preparation.Theβ-peptide polymer demonstrated quorum sensing(QS)interference and bactericidal effect against both bacterial communities and individual bacterium to simultaneously block bacterial communication and disrupt bacterial membranes.The hierarchical QS network was suppressed,and main QS signaling systems showed considerably down-regulated gene expression,resulting in excellent biofilm eradication and virulence reduction effects.The dual-modal antibacterial ability possessed excellent therapeutic effects in Pseudomonas aeruginosa pneumonia,which could inhibit biofilm formation and exhibit better antibacterial and anti-inflammatory efficiency than clinically used antibiotics,levofloxacin.Furthermore,theβ-peptide polymer also showed excellent therapeutic effect Escherichia coli pyogenic liver abscess.Together,we believed that theβ-peptide polymer had a feasible clinical potential to treat bacterial infection diseases. | Wanlin Li Ximian Xiao Yuchen Qi Xiuhui Lin Huiqun Hu Minqi Shi Min Zhou Weinan Jiang Longqiang Liu Kang Chen Kai Wang Runhui Liu Min Zhou | 2023 | Research2023，，4： | 0 |
| 16 | Reliability analysis of retractable drill bit with air reverse circulation used for drilling while casing显示文摘Casing-while-drilling(CWD)with down the hole(DTH)hammer drilling technology has been widely used in unconsolidated formations,due to its advantages in protecting the borehole wall,excellent rock cuttings capacity,and fast penetration rate in hard rock.As an important component of the CWD system,the structure of the retractable drill bit needs not only to ensure to form stronger reverse circulation,but also to be expandable or retractable as needed,otherwise the drill bit cannot be lift and put down smoothly in the casing,and may lead to drilling accidents.This paper developed a new type of reverse circulation DTH hammer drill bit used for CWD drilling technology.The retractable performance of this type of drill bit is studied using ADAMS software.The results show that it is smoothly expandable and retractable as designed under the conditions of the weight of the bit(WOB)of 0.5–2.0 t and the rotation speed of 30–60 r/min.To investigate the reverse circulation effect of the drill bit,Fluent software was used to simulate the flow characteristic inside it.The simulation results indicated that it can form strong reverse circulation,and the entrainment ratio h can reach 9.5%. | WANG Minqi YIN Qilei CHEN Baoyi QI Bo BO Kun CAO Pinlu | 2023 | Global Geology2023，26，1： | 0 |
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