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| 1 | Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers显示文摘It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices.Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes.Our recent report on lithium hexamethyldisilazide-initiated superfast polymerization on amino acid N-carboxyanhydrides enables the quick synthesis of host defense peptide-mimicking peptide polymers.Here we reported a facile and cost-effective thermoplastic polyurethane(TPU)surface modification of peptide polymer(DLL:BLG=90:10)using plasma surface activation and substitution reaction between thiol and bromide groups.The peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in vitro.Furthermore,the peptide polymer-modified TPU surfaces showed excellent biocompatibility,displaying no hemolysis and cytotoxicity.In vivo study using methicillin-resistant Staphylococcus aureus(MRSA)for subcutaneous implantation infectious model showed that peptide polymer-modified TPU surfaces revealed obvious suppression of infection and great histocompatibility,compared to bare TPU surfaces.We further explored the antimicrobial mechanism of the peptide polymer-modified TPU surfaces,which revealed a surface contact-killing mechanism by disrupting the bacterial membrane.These results demonstrated great potential of the peptide-modified TPU surfaces for practical application to combat bacterial infections that are associated with implantable materials and devices. | Ziyi Lu Yueming Wu Zihao Cong Yuxin Qian Xue Wu Ning Shao Zhongqian Qiao Haodong Zhang Yunrui She Kang Chen Hengxue Xiang Bin Sun Qian Yu Yuan Yuan Haodong Lin Meifang Zhu Runhui Liu | 2021 | Bioactive Materials2021，6，12： | 5 |
| 2 | Biomarkers in the early period of acute myocardial infarction in rat serum and protective effects of Shexiang Baoxin Pill using a metabolomic method显示文摘 | Peng Jiang Weixing Dai Shikai Yan Zhongliang Chen Ruilin Xu Jianmi Ding Li Xiang Shuping Wang Runhui Liu Weidong Zhang | 2011 | Journal of Ethnopharmacology2011，，2： | 2 |
| 3 | Plasma pharmacochemistry based approach to screening potential bioactive components in Huang-Lian-Jie-Du-Tang using high performance liquid chromatography coupled with mass spectrometric detection显示文摘 | Yaohua Hu Peng Jiang Shuping Wang Shikai Yan Li Xiang Weidong Zhang Runhui Liu | 2011 | Journal of Ethnopharmacology2011，，2： | 1 |
| 4 | Simultaneous analysis of alkaloids from Zanthoxylum nitidum by high performance liquid chromatography–diode array detector–electrospray tandem mass spectrometry显示文摘 | Mingjin Liang Weidong Zhang Jiang Hu Runhui Liu Chuan Zhang | 2006 | Journal of Pharmaceutical and Biomedical Analysis2006，，2： | 1 |
| 5 | Breaking or following the membrane-targeting mechanism:Exploring the antibacterial mechanism of host defense peptide mimicking poly(2-oxazoline)s显示文摘Peptides exert important biological functions but their application is hindered by their susceptibility to proteolysis and poor stability in vivo.Thus,functional peptide mimics have drawn a great deal of attention to address this challenge.Poly(2-oxazoline)s,a class of biocompatible and proteolysis-resistant polymer,can work as host defense peptide mimics without following the general membrane-targeting mechanism as shown in our previous work.This observation encouraged us to figure out if poly(2-oxazoline)s are special and break the general membrane-targeting mechanism of host defense peptides and their mimics.In this study,we aimed at the connection between structure and antibacterial mechanism of poly(2-oxazoline)s.A new γ-aminobutyric acid(GABA)-pendent poly(2-oxazoline)was synthesized and investigated to compare with glycine-pendent poly(2-oxazoline)in our previous study,with the former polymer has two extra CH2 groups in the sidechain to increase the hydrophobicity and amphiphilicity.Membrane depolarization assay suggested that incorporating two more CH2 groups into the sidechain of poly(2-oxazoline)resulted in a mechanism switch from DNA-targeting to membrane-targeting,which was supported by the slow time-kill kinetics and slightly distorted and sunken membrane morphology.Besides,GABA-pendent poly(2-oxazoline)showed potent activity against methicillin-resistant S.aureus and low hemolysis on human red blood cells.Moreover,repeated use of the antimicrobial poly(2-oxazoline)did not stimulate bacteria to obtain resistance,which was an obvious advantage of membrane-targeting antimicrobial agents. | Chengzhi Dai Min Zhou Weinan Jiang Ximian Xiao Jingcheng Zou Yuxin Qian Zihao Cong Zhemin Ji Longqiang Liu Jiayang Xie Zhongqian Qiao Runhui Liu | 2020 | Journal of Materials Science & Technology2020，59，24： | 1 |
| 6 | Alkali-metal hexamethyldisilazide initiated polymerization on alpha-amino acid N-substituted N-carboxyanhydrides for facile polypeptoid synthesis显示文摘Polypeptoids have been explored as mimics of polypeptides,owing to polypeptoids'superior stability upon proteolysis.Polypeptoids can be synthesized from one-pot ring-opening polymerization of amino acid N-substituted N-carboxyanhydrides(NNCAs).However,the speed of polymerization of NNCAs can be very slow,especially for NNCAs bearing a bulky N-substitution group.This hindered the exploration on polypeptoids with more diverse structures and functions.Therefore,it is in great need to develop advanced strategies that can accelerate the polymerization on inactive NNCAs.Hereby,we report that lithium/sodium/potassium hexamethyldisilazide(Li/Na/KHMDS)initiates a substantially faster polymerization on NNCAs than do commonly used amine initiators,especially for NNCAs with bulky N-substitution group.This fast NNCA polymerization will increase the structure diversity and application of polypeptoids as synthetic mimics of polypeptides. | Yueming Wu Min Zhou Kang Chen Sheng Chen Ximian Xiao Zhemin Ji Jingcheng Zou Runhui Liu | 2021 | Chinese Chemical Letters2021，32，5： | 1 |
| 7 | Protective Effects of Huang-Lian-Jie-Du-Tang against Polymicrobial Sepsis Induced by Cecal Ligation and Puncture in Rats显示文摘 | Yufen Wei Lei Shan Liming Qiao Runhui Liu Zhenlin Hu Weidong Zhang Seung-Heon Hong | 2013 | Evidence-Based Complementary and Alternative Medicine2013，，： | 1 |
| 8 | The inhibitory activities of the components of Huang-Lian-Jie-Du-Tang (HLJDT) on eicosanoid generation via lipoxygenase pathway显示文摘 | Huawu Zeng Shengshan Dou Jing Zhao Siyang Fan Xing Yuan Shuanglai Zhu Li Li Weidong Zhang Runhui Liu | 2011 | Journal of Ethnopharmacology2011，，2： | 1 |
| 9 | Host defense peptide-mimicking peptide polymer-based antibacterial hydrogel enables efficient healing of MRSA-infected wounds显示文摘Wound infections are a compelling health issue caused by the invasion and proliferation of pathogens in wound sites.Antibioticloaded hydrogels are widely used to achieve anti-infectious wound healing.However,due to the quick emergence of drugresistant bacteria,such as methicillin-resistant Staphylococcus aureus(MRSA),wound infection has been a formidable challenge to human health.To address MRSA-infected wounds,an antibacterial peptide polymer-loaded hyaluronic acid(HA)hydrogel(Gel-HA@P)is prepared.The peptide polymer is designed to mimic host defense peptides as the antibiotic alternative showing potent antibacterial activity,low susceptibility to drug resistance and good stability against proteolysis.HA is biocompatible and biodegradable hydrogel substrate as a primary constituent of the extracellular matrix and suitable for cell migration and wound healing.Gel-HA@P shows potent activity against MRSA in vitro and in vivo,low toxicity during the treatment and promotes the wound healing in vivo.This design has proven to be an effective and antibiotic-free strategy to enable the healing of MRSA-infected wounds. | Zhongqian Qiao Wenjing Zhang Yueming Wu Weinan Jiang Ning Shao Jiayang Xie Guixue Xia Qimeng Chen Zhefeng Liu Jingcheng Zou Jiawei Gu Shifang Luan Haodong Lin Runhui Liu | 2023 | Science China Chemistry2023，66，6： | 1 |
| 10 | Synergistic Combination of Biodegradable Peptide Polymer and Curcumin as Promising Antibiotic Substitution in Aquaculture to Alleviate the Global Challenge of Antimicrobial Resistance显示文摘The massive use of antibiotics in aquaculture and resulted antibacterial resistance problem urgently need antibiotic substitutions. Herein, we report a promising substitution of aquaculture antibiotics using a synergistic combination of biodegradable peptide polymers and curcumin, a natural compound from plant. The synergistic combination shows strong antibacterial activity against V. fluvialis and some other common bacteria in aquaculture. The membrane-damaging antibacterial mechanism echoes our finding that the synergistic combination will not induce bacteria to develop resistance after continuous use. The synergistic combination also displays effective cure on V. fluvialis-infected zebrafish. The biodegradability of the peptide polymer enables the combination to lose antibacterial activity and will not cause selective pressure on bacterial in the environment. Our study indicates potential application of synergistic composition, biodegradable peptide polymer and curcumin, as promising antibiotic substitution in aquaculture, which represents a promising strategy to address the global challenge of antimicrobial resistance. | Pengcheng Ma Yuan Chen Ning Shao Junyu Zhang Yueming Wu Runhui Liu | 2022 | Chinese Journal of Chemistry2022，40，24： | 1 |
| 11 | Therapeutic strategies against bacterial biofilms显示文摘The emergence of multi-drug resistance makes bacterial infection a major threat to public health and economy.The formation of bacterial biofilms is one of the main reasons of bacterial resistance.The complexity of chemical composition and physical structure makes the elimination of mature biofilms a difficult problem.The highly antibiotic resistant property of biofilms urgently calls for potent antimicrobial agents and novel antibiofilm strategies.Researchers have made a lot of efforts in this field.Here we review the current strategies to eliminate mature biofilms and progress in related drug delivery nanosystems,with the aim of inspiring researchers to design new antibiofilm systems. | Yufang Bi Guixue Xia Chao Shi Jianglin Wan Longqiang Liu Yuan Chen Yueming Wu Wenjing Zhang Min Zhou Hongyan He Runhui Liu | 2021 | Fundamental Research2021，1，2： | 1 |
| 12 | Open-vessel Synthesis of Poly-N-methoxyethylglycine显示文摘Polypeptoids are widely used in biological applications owing to their diverse functions and proteolytic stability.One type of polypeptoids,poly-N-methoxyethylglycine(P-Nmeg),has been found to possess remarkable hydrophilicity and notable properties in terms of protein,cell,and bacterial antifouling.However,the currently known synthesis methods of P-Nmeg include solid-phase synthesis,which is time-consuming and difficult to scale up,and N-substituted N-carboxyanhydride(NNCA)ring-opening polymerization,whose monomers were difficult to store.In this study,we used the chemical stable Nmeg N-phenoxycarbonyl(NPC)as the monomer,which was obtained without the use of highly toxic reactants,such as phosgene or phosphorus halide,to synthesize P-Nmeg under open-vessel conditions.By adding BnNH_(2) as an initiator at 80℃,we can obtain controllable short chain length P-Nmeg with narrow dispersity. | LIU Guojan MA Ke CHEN Qi ZHANG Donghui LIU Runhui | 2024 | Chemical Research in Chinese Universities2024，40，1： | 0 |
| 13 | Facile fabrication of durable superhydrophobic fabrics by silicon polyurethane membrane for oil/water separation显示文摘Nowadays, oil contamination has become a major reason for water pollution, and presents a global environmental challenge. Although many efforts have been devoted to the fabrication of oil/water separation materials, their practical applications are still hindered by their weak durability, poor chemical tolerance,environmental resistance, and potential negative impact on health and the environment. To overcome these drawbacks, this work offers a facile method to fabricate the eco-friendly and durable oil/water separation membrane fabrics by alkaline hydrolysis and silicon polyurethane coating. The X-ray photoelectron spectroscopy, scanning electron microscopy, and atomic force microscopy results demonstrate that silicon polyurethane membrane could be coated onto the surface of hydrolyzed polyester fabric and form a micro-/nano-scaled hierarchical structure. Based on this, the modified fabric could have a stable superhydrophobic property with a water contact angle higher than 150°, even after repeated washing and mechanical abrasion 800 times, as well as chemical corrosion. Moreover, the modified fabrics show excellent oil/water separation efficiency of up to 99% for various types of oil–water mixture. Therefore, this durable, eco-friendly and cost-efficient superhydrophobic fabric has great potential in large-scale oil/water separation. | Taoyan Mao Runhui Xiao Peng Liu Jiale Chen Junqiang Luo Su Luo Fengwei Xie Cheng Zheng | 2023 | Chinese Journal of Chemical Engineering2023，55，3： | 0 |
| 14 | Efficient adaptation of high-quality international guidelines for Chinese children with primary immune thrombocytopenia显示文摘Over the last few decades,a plethora of guidelines for primary immune thromboeytopenia(ITP)in children have been developed and published.However,the guidelines developed by one country or organization are not always appropriate to other countries or regions due to differences in local circumstances. | Yali Liu Runhui Wu | 2022 | Pediatric Investigation2022，6，2： | 0 |
| 15 | Pharmacokinetic variability of factor VIII concentrates in Chinese pediatric patients with moderate or severe hemophilia A显示文摘Importance:The use of factor VIII(FVIII)concentrates under pharmacokinetic(PK)guidance has become the main approach for treatment of hemophilia.However,limited PK research has been conducted in Chinese pediatric patients.Objective:To investigate the PK parameters of various FVIII concentrates in Chinese pediatric patients.Methods:Seventy-nine patients were enrolled(28 treated with Kogenate FS^(®),23 treated with Advate^(®),and 28 treated with GreenMono^(™)).All enrolled patients participated in single-dose PK analysis after at least a 3-day washout period.Blood samples were collected predose,as well as at 1 h,9 h,24 h,and 48 h after infusion;FVIII levels were measured using a one-stage clotting assay,von Willebrand Factor Antigen(VWF:Ag)levels and blood types were also determined.PK parameters were evaluated by WAPPS-Hemo.Results:Mean values of terminal elimination half-life time(t_(1/2))for the Kogenate FS^(®),Advate^(®),and GreenMono^(™)FVIII groups were 12.24 h,10.18 h,and 9.62 h;median clearance values were 4.16,6.23,and 5.11 mL kg^(-1)h^(-1);and median in vivo recovery values were 1.97,1.55,and 1.61 IU/dL per IU/kg.Longer t_(1/2),higher in vivo recovery,and lower clearance were observed in patients with higher VWF:Ag level who were treated with recombinant concentrates.Interpretation:Chinese pediatric patients with hemophilia had FVIII PK characteristics similar to those previously observed in non-Chinese children,including large variation among individuals.VWF:Ag level and FVIII brand were associated with differences in FVIII PK.Thus,PK-guided dosing should be used to optimize individualized therapy in Chinese children. | Zhenping Chen Kun Huang Gang Li Yingzi Zhen Xinyi Wu DiAi Guoqing Liu Zekun Li Iorio Alfonso Runhui Wu | 2021 | Pediatric Investigation2021，5，1： | 0 |
| 16 | Sensitive detection of microRNAs using polyadenine-mediated fluorescent spherical nucleic acids and a microfluidic electrokinetic signal amplification chip显示文摘The identification of tumor-related microRNAs(miRNAs)exhibits excellent promise for the early diagnosis of cancer and other bioanalytical applications.Therefore,we developed a sensitive and efficient biosensor using polyadenine(polyA)-mediated fluorescent spherical nucleic acid(FSNA)for miRNA analysis based on strand displacement reactions on gold nanoparticle(AuNP)surfaces and electrokinetic signal amplification(ESA)on a microfluidic chip.In this FSNA,polyA-DNA biosensor was anchored on AuNP surfaces via intrinsic affinity between adenine and Au.The upright conformational polyA-DNA recognition block hybridized with 6-carboxyfluorescein-labeled reporter-DNA,resulting in fluorescence quenching of FSNA probes induced by AuNP-based resonance energy transfer.Reporter DNA was replaced in the presence of target miRNA,leading to the recovery of reporter-DNA fluorescence.Subsequently,reporter-DNAs were accumulated and detected in the front of with Nafion membrane in the microchannel by ESA.Our method showed high selectivity and sensitivity with a limit of detection of 1.3 pM.This method could also be used to detect miRNA-21 in human serum and urine samples,with recoveries of 104.0%-113.3% and 104.9%-108.0%,respectively.Furthermore,we constructed a chip with three parallel channels for the simultaneous detection of multiple tumor-related miRNAs(miRNA-21,miRNA-141,and miRNA-375),which increased the detection efficiency.Our universal method can be applied to other DNA/RNA analyses by altering recognition sequences. | Jun Xu Qing Tang Runhui Zhang Haoyi Chen Bee Luan Khoo Xinguo Zhang Yue Chen Hong Yan Jincheng Li Huaze Shao Lihong Liu | 2022 | Journal of Pharmaceutical Analysis2022，12，5： | 0 |
| 17 | Host defense peptide-mimickingβ-peptide polymer displaying strong antibacterial activity against cariogenic Streptococcus mutans显示文摘Cariogenic Streptococcus mutans(S.mutans)is a leading cause of bacterial-induced oral diseases.Current strategies to kill bacteria based on Host defense peptide(HDP)mimicking polymers hold promise to treat oral bacterial infection.Here,we explore the impact of hydrophobic subunit and chain length variation on the antibacterial and antibiofilm activity ofβ-peptide polymers.The physicochemical and biological prop-erties,such as the toxicity,the antibacterial activity,and the effect on bacterial transcription ofβ-peptide polymers,were systematically investigated with numerous techniques.The results exhibited that the op-timalβ-peptide polymer has low toxicity towards human periodontal ligament fibroblasts,andβ-peptide polymers(especially P3)have more excellent antibacterial activity against S.mutans than metronidazole.In addition,β-peptide polymers inhibited the reversible and irreversible bacterial adhesion during the formation of biofilms.The polymer can promote biofilm dispersion by decreasing the hydrophobicity of bacterial cells after adhering to cell surfaces.Analysis of the transcriptome for S.mutans treated withβ-peptide polymers demonstrated thatβ-peptide polymers could reduce the cariogenicity of S.mutans by impacting the transcription of the energy and acid metabolism-related genes.β-peptide polymers are promising antimicrobial agents in clinical dentistry due to their high antibacterial efficiency and low tox-icity. | Yi Yang Yuxin Qian Mingxing Zhang Shuang Hao Hui Wang Yongqiang Fan Runhui Liu Dake Xu Fuhui Wang | 2023 | Journal of Materials Science & Technology2023，，2： | 0 |
| 18 | Host-Defense-Peptide-Mimickingβ-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously显示文摘Host defense peptides(HDPs)are one of the potentially promising agents for infection diseases due to their broad spectrum and low resistance rate,but their clinical applications are limited by proteolytic instability,high-cost,and complicated synthesis process.Here,we report a host-defense-peptidemimickingβ-peptide polymer that resists proteolysis to have enhanced the activity under physiological conditions,excellent antimicrobial efficiency even at high density of bacteria,and low cost for preparation.Theβ-peptide polymer demonstrated quorum sensing(QS)interference and bactericidal effect against both bacterial communities and individual bacterium to simultaneously block bacterial communication and disrupt bacterial membranes.The hierarchical QS network was suppressed,and main QS signaling systems showed considerably down-regulated gene expression,resulting in excellent biofilm eradication and virulence reduction effects.The dual-modal antibacterial ability possessed excellent therapeutic effects in Pseudomonas aeruginosa pneumonia,which could inhibit biofilm formation and exhibit better antibacterial and anti-inflammatory efficiency than clinically used antibiotics,levofloxacin.Furthermore,theβ-peptide polymer also showed excellent therapeutic effect Escherichia coli pyogenic liver abscess.Together,we believed that theβ-peptide polymer had a feasible clinical potential to treat bacterial infection diseases. | Wanlin Li Ximian Xiao Yuchen Qi Xiuhui Lin Huiqun Hu Minqi Shi Min Zhou Weinan Jiang Longqiang Liu Kang Chen Kai Wang Runhui Liu Min Zhou | 2023 | Research2023，，4： | 0 |
| 19 | Dual Functional Diblock Amino Acid Copolymer Displaying Synergistic Effect with Curcumin against MRSA and Encapsulation of Curcumin显示文摘The intensive use of antibiotics intensifies the development of bacterial resistance,which has become a serious problem globally.Methicillin-resistant Staphylococcus aureus(MRSA)has resulted in significant morbidity and mortality.Therefore,it is an urgent need to develop new antimicrobial drugs and administration methods.Herein,we report a dual functional diblock copolymer PLL20-b-PBLG20,which was prepared by superfast and water-insensitive polymerization on N-carboxyanhydrides(NCA)initiated by tetraalkylammonium carboxylate.In addition to direct antimicrobial activity,PLL20-b-PBLG20 also exerts a synergistic bactericidal effect against MRSA with curcumin,a plant extract with antibacterial property.Moreover,PLL20-b-PBLG20 successfully encapsulates curcumin to form nanoparticles via self-assembly.The combination of dual functional PLL20-b-PBLG20 and curcumin holds promise in combating MRSA infections. | Yueming Wu Yuan Chen Haowen Yu Alideertu Dong Runhui Liu | 2023 | Chinese Journal of Chemistry2023，41，23： | 0 |
| 20 | In vitro and in vivo evaluation of implantable bacterial-killing coatings based on host defense peptides and their synthetic mimics显示文摘Contact-killing antimicrobial coatings based on host defense peptides(HDPs) and their synthetic mimics have shown potential as powerful tools to combat implant-associated infections. Covalent modification of the antimicrobial surface has been utilized to prevent early-stage microbial infections owing to the less drug-leaching possibility that is beneficial to human health and the natural environment. Although considerable progress has been achieved in preparing contact-killing antimicrobial surfaces, discussions focusing on the in vitro and in vivo evaluations of these surfaces are limited. In this review, we summarized the established in vitro methods to simulate the practical interaction of microbes with the surrounding biological environment and the reported in vivo studies at different implant sites. We suggested that the in vivo specific site infection model is essential to gain a comprehensive understanding of these antimicrobial coatings in the preclinical stage, which can be established based on investigations performed using various in vitro assays and conventional non-specific site infection models. Overall, these precedent studies focusing on bacterial contact-killing coatings modified with HDPs and HDP mimics can be considered as critical to assess the surface antibacterial ability and to guide the future developments and applications of antimicrobial surfaces. | Yuxin Qian Shuai Deng Xue Wu Yunrui She Runhui Liu Haodong Lin | 2021 | Journal of Materials Science & Technology2021，，32： | 0 |
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