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| 1 | Charlson comorbidity index helps predict the risk of mortality for patients with type 2 diabetic nephropathy显示文摘研究目的:探讨Charlson合并症指数(CCI)对2型糖尿病肾病死亡率的预测作用。研究方法:建立2型糖尿病肾病研究队列,根据CCI评分将患者合并症的严重程度分为三度:轻度(CCI1–2分)、中度(CCI 3–4分)、重度(CCI≥5分)。将影响死亡率的因素及按CCI分层后的组间差异指标进行Logistic回归分析及方差分析(ANOVA)。Kaplan-Meier生存曲线分析CCI指数对生存时间及死亡率的影响。重要结论:533例2型糖尿病肾病患者纳入研究。所有患者的CCI评分均大于1,44.7%(238/533)的患者死亡。患者的死亡率随CCI评分增加而增加,CCI 1–2分患者的死亡率为21.0%(50/238),CCI 3–4分患者的死亡率为56.7%(135/238),CCI≥5分患者的死亡率为22.3%(53/238)。Logistic回归分析显示CCI评分、血红蛋白和血浆白蛋白水平是患者死亡率的预测因子(P<0.05)。方差分析结果显示,与CCI评分相对较低的患者相比,CCI评分越高的患者其血红蛋白水平较低,而血清肌酐较高,死亡率也更高。Kaplan-Meier生存曲线显示CCI评分越高的患者生存时间越短。CCI评分是一种简单易行且实用的评估疾病合并症的方法,可用于预测2型糖尿病肾病患者的死亡率。关注2型糖尿病肾病患者的合并症将有利于早期、有效治疗及改善预后。 | You-qun HUANG Rong GOU Yong-shu DIAO Qing-hua YIN Wen-xing FAN Ya-ping LIANG Yi CHEN Min WU Li ZANG Ling LI Jing ZANG Lu CHENG Ping FU Fang LIU | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，1： | 23 |
| 2 | Cationic nanocarriers induce cell necrosis through impairment of Na^+/K^+-ATPase and cause subsequent inflammatory response显示文摘有积极表面费用的 Nanocarriers 以他们限制了他们的临床的应用的毒性著名。位于他们的毒性下面的机制例如煽动性的反应的正式就职,仍然保持大部分未知。在我们发现了 cationic nanocarriers 的那注射的现在的学习,包括 cationic liposomes, PEI,和 chitosan,导致了坏死的房间的快速的外观。cationic nanocarriers 导致的房间坏死依赖于他们的积极表面费用,但是不要求 RIP1 和 Mlkl。相反,细胞内部的 Na + 超载被发现伴随房间死亡。在文化媒介或有 Na +/K+-ATPase 阳离子绑定地点禁止者 ouabain 的房间的预告的处理的 Na + 的弄空,从房间坏死的保护的房间。而且,有 cationic nanocarriers 的处理在 vitro 并且在 vivo 禁止了 Na +/K+-ATPase 活动。计算模拟证明 cationic 搬运人能与 Na +/K+-ATPase 的阳离子绑定地点交往。有 ouabain 的小剂量的老鼠 pretreated 在 cationic nanocarriers 的致命的剂量的注射以后显示出改进幸存。进一步的分析建议 cationic nanocarriers 和 mitochondrial DNA 的产生的漏导致的那房间坏死能在 vivo 触发严重发炎,它被包含 TLR9 并且 MyD88 发信号的一条小径调停。一起拿,我们的结果揭示由此的新奇机制 cationic nanocarriers 通过和 mitochondrial 的随后的暴露的和 Na +/K+-ATPase, 的相互作用导致尖锐房间坏死是的联系损坏的分子的模式调停的一个关键事件煽动性的回答。我们的学习为评估 nanocarriers 的 biocompatibility 并且为药交货设计更好、更安全的有重要含意。 | Xiawei Wei Bin Shao Zhiyao He Tinghong Ye Min Luo Yaxiong Sang Xiao Liang Wei Wang Shuntao Luo Shengyong Yang Shuang Zhang Changyang Gong Maling Gou Hongxing Deng Yinglan Zhao Hanshuo Yang Senyi Deng Chengjian Zhao Li Yang Zhiyong Qian Jiong Li Xun Sun Jiahuai Han Chengyu Jiang Min Wu Zhirong Zhang | 2015 | Cell Research2015，25，2： | 21 |
| 3 | Effect of anaphylatoxin C3a, C5a on the tubular epithelial-myofibroblast transdifferentiation in vitro显示文摘背景 Tubulointerstitial 肾的纤维变性是进步的肾疾病的普通结束点,并且管状的 epithelial-myofibroblast transdifferentiation ( TEMT )在 tubulointerstitial 的进步起一个关键作用肾的 fibrosis.Anaphylatoxin C3a 和 C5a 在肾的疾病并且作为这研究的潜在的新治疗学的 targets.The 目的作为新奇 profibrotic 因素被识别是调查是否 C3a , C5a 能由转变生长 factor-31 ( TGF-1 ) /connective 织物生长因素调整 TEMT 控制组, 10 ng/ml TGF-31 组, 50 nmol/L C5a 组,加 2.5 mol/L C5aRA 的 50 nmol/L C5a group.TGF-1 受体对手( TGF-1 RA ) 10 g/ml 被用来调查 导致C3a 、 导致C5a 的 TEMT.Electron 的机制显微镜学被用来观察染色的词法 changes.Immunocytochemistry ,即时 PCR 并且西方的弄污被用来检测光滑的肌肉肌动朊的表情( -SMA),E-cadherin,Col-I,C3a 受体( C3aR ), C5aR , CTGF 和与 C3a 和 C5a fo 有教养的 TGF-1.Results HK-2 | LIU Fang GOU Rong HUANG Jun FU Ping CHEN Feng FAN Wen-xing HUANG You-qun ZANG Li WU Min QIU Hong-yu WEI Da-peng | 2011 | Chinese Medical Journal2011，，23： | 15 |
| 4 | Pharmacological Inhibition of Macrophage Toll-like Receptor 4/Nuclear Factor-kappa B Alleviates Rhabdomyolysis-induced Acute Kidney Injury显示文摘 | Rong-Shuang Huang Jiao-Jiao Zhou Yu-Ying Feng Min Shi Fan Guo Shen-Ju Gou Stephen Salerno Jr. Liang Ma Ping Fu | 2017 | Chinese Medical Journal2017，，18： | 9 |
| 5 | Establishment of a transgenic mouse model with liver-specific expression of secretory immunoglobulin D显示文摘Mutation of mevalonate kinase(MVK) is thought to account for most cases of hyperimmunoglobulinemia D syndrome(HIDS) with recurrent fever.However,its mechanism and the relationship between elevated serum immunoglobulin D(IgD) and the clinical features of HIDS are unclear.In this study,we generated by fusion PCR a vector to express high levels of chimeric secretory IgD(csIgD) specifically in the liver.We then generated seven founder lines of transgenic mice by co-microinjection,and verified them using genomic PCR and Southern blotting.We detected the expression of csIgD by reverse transcription PCR,quantitative PCR,western blotting,and enzyme-linked immunosorbent assays.We demonstrated that csIgD could be specifically and stably expressed in the liver.We used flow cytometry to show that overexpression of csIgD in the bone marrow and spleen cells had no effect on B cell development.Morphologic and anatomical observation of the transgenic mice revealed skin damage,hepatosplenomegaly,and nephromegaly in some transgenic mice;in these mice,pathological sections showed high levels of cell necrosis and protein-like sediments in the liver,spleen,and kidney.We demonstrated that the genomic insertion sites of the transgenes did not disrupt the MVK gene on mouse chromosome 5.This transgenic mouse will be useful to explore the pathogenesis of HIDS. | WANG Ping WEI ZhiGuo YAN BoWen HUANG Tan GOU KeMian DAI YunPing ZHENG Min WANG MeiLi CHENG XueQian WANG XiFeng XU Chen SUN Yi | 2012 | Science China(Life Sciences)2012，55，3： | 6 |
| 6 | Mechanical and fatigue properties of self-piercing riveted joints in high-strength steel and aluminium alloy显示文摘Static tensile and fatigue tests were performed on shear and tensile self-piercing riveted aluminium-steel structures to evaluate their mechanical and fatigue properties. The influences of the thickness and the strength of the highstrength steel on mechanical and fatigue performances were investigated based on the tensile and F-N curves of the joints. The results show that mechanical and fatigue properties of the shear self-piercing riveted joints are much better than those of the tensile self-piercing riveted joints. Mechanical and fatigue performances of the two joints were significantly influenced by the thickness and strength of the steel sheet,and were markedly improved when the thickness of steel sheet increased. The steel strength showed significantly different effects on shear and tensile riveted structures,i. e.,when the steel strength increased,the strength of the shear structure greatly increased while the tensile structure just had a slight increase in the strength. Fatigue failure generally occurred in the sheet materials and the fatigue crack location changed with increasing the sheet thickness and the sheet strength. | Chun-yu Zhang Rui-bin Gou Min Yu Ya-jing Zhang Yin-hu Qiao Shu-ping Fang | 2017 | Journal of Iron and Steel Research(International)2017，24，2： | 6 |
| 7 | Combinatorial effects of conception and governor vessel electroacu-puncture and human umbilical cord blood-derived mesenchymal stem cells on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion显示文摘OBJECTIVE: To investigate the combinatorial effects of conception and governor vessel electroacupuncture(EA) and human umbilical cord blood-derived mesenchymal stem cells(HUCB-MSCs) on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion. METHODS: With the HUCB-MSCs isolated, cultured and identified and the models of cerebral ischemia-reperfusion established, the HUCB-MSCs of passage three were intracranially transplanted and the EA at conception and governor vessels was applied. The pathomorphologic lesion by hematoxylin-eosin staining and the cellular apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling method around the ischemic focus were observed. RESULTS: The cultured adherent HUCB-MSCs exhibited a spindle shape and expressed MSC-specific markers, with the cell purity and proliferation rate significantly increasing after the primary passage. HE staining showed that there were no pathological changes observed in the sham surgery group. However, in the PBS transplantation group, degeneration and necrosis of a great number of nerve cells were seen. In both the HUCB-MSCs transplantation group and the HUCB-MSCs transplantation + EA group, reparative changes of the pathomorphism of the tissue were found. Both combination treatment and simple MSCs treatment were able to improve the pathomorphorlogic lesion following cerebral ischemia and reduce the abnormal TUNEL-positive numbers, with former better than latter. CONCLUSION: HUCB-MSCs improve pathological lesions and inhibit the cellular apoptosis around the cerebral ischemic area. EA at conception and governor vessels also improve pathological lesion and inhibit the cellular apoptosis in rats treated with HUCB-MSCs transplantation, which effects were superior to that of simple HUCB-MSCs transplantation. | Zhuoxin Yang Pengdian Chen Haibo Yu Wenshu Luo Min Pi Yonggang Wu Ling Wang Fuxia Yang Yanhua Gou | 2013 | Journal of Traditional Chinese Medicine2013，33，6： | 5 |
| 8 | Total Energy Expenditure of 16 Chinese Young Men Measured by the Doubly Labeled Water Method显示文摘Objective Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±1 years with body mass index 22.0±1.4 kg/m 2 were recruited. TEE was measured by the DLW method, and basal energy expenditure (BEE) was determined by indirect calorimetry. We also conducted 24-h activity, energy balance and factorial approach to estimate energy requirements of the subjects. Results TEE of subjects by DLW method was 9.45±0.57 MJ/day (2258±180 kcal/day). The 24-h activity was 10.80±0.33 MJ/day (2582±136 kcal/day). The energy requirement, derived from energy balance observations, was 9.93±1.32 MJ/day (2373±315 kcal/day). The BEE of 6.65±0.28 MJ/day (1589±67 kcal/day), calculated by the adjusted Schofield equation, was significantly higher (P<0.001) than that measured by indirect calorimetry, 5.99±0.66 MJ/day (1433±158 kcal/day). The TEE derived from the factorial approach was 10.31±0.43 MJ/day (2463±104 kcal/day). Conclusion The TEE of Chinese young men measured by the DLW method was about 10% lower than the current recommended nutrient intake (RNI), suggesting that the RNI for Chinese men maybe overestimated. Further studies are warranted to determine the value of the estimated energy requirement. | ZHUO Qin SUN Rui GOU Ling Yan PIAO Jian Hua LIU Jian Min TIAN Yuan ZHANG Yu Hui YANG Xiao Guang | 2013 | Biomedical and Environmental Sciences2013，26，6： | 4 |
| 9 | Microbial community structure and dynamics of starch-fed and glucose-fed chemostats during two years of continuous operation显示文摘微生物引起的社区二 mesophilic 组织厌氧的 chemostats,用葡萄糖喂的,其它与是的淀粉,唯一的碳采购原料,在二年期间以各种各样的冲淡率(为葡萄糖的 0.050.25 d 1 和为淀粉的 0.0250.1 d 1) 被学习连续操作。在喂葡萄糖的 chemostat, aceticlastic methanogen Methanosaeta spp。并且 hydrogenotrophic methanogen Methanoculleus spp。以低冲淡率支配了,而 Methanosaeta spp。并且 hydrogenotrophic Methanobacterium spp。当冲淡率比 0.1 d 1 大时,一起支配了。细菌与分别地以 0.05, 0.1,和 0.15 d 1, 的冲淡率支配的数 Bacteroidetes,螺旋菌,和 Actinobacteria 结盟了,当在更高的冲淡支配的 Firmicutes 评价时(0.2 和 0.25 d 1) 。在喂淀粉的 chemostat, aceticlastic 和 hydrogenotrophic methanogens 根本共存了冲淡率。尽管属于仅仅二个数的细菌为淀粉降级(以以另外的冲淡率的 0.08 d 1 和 Firmicutes 的冲淡率的螺旋菌) 主要负责,不同的细菌的类以不同冲淡率被识别。与在喂葡萄糖的 chemostat 的 Archaea 的例外,乐队模式由使中毒表明在二 chemostats 的微生物引起的社区的坡度胶化电气泳动(DGGE ) 以经常的冲淡率在长期的操作期间显示了显著变化。细菌的社区处于冲淡率随着变化变化了,并且在在喂葡萄糖、喂淀粉的 chemostats 的长期的操作期间是乖僻的。 | Min GOU Jing ZENG Huizhong WANG Yueqin TANG Toru SHIGEMATSU Shigeru MORIMURA Kenji KIDA | 2016 | Frontiers of Environmental Science & Engineering2016，10，2： | 3 |
| 10 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 11 | Key regulators of intestinal stem cells:diet,microbiota,and microbial metabolites显示文摘Interactions between diet and the intestinal microbiome play an important role in human health and disease development.It is well known that such interactions,whether direct or indirect,trigger a series of metabolic reactions in the body.Evidence suggests that intestinal stem cells(ISCs),which are phenotypic precursors of various intestinal epithelial cells,play a significant role in the regulation of intestinal barrier function and homeostasis.The advent and evolution of intestinal organoid culture techniques have presented a key opportunity to study the association between the intestinal microenvironment and ISCs.As a result,the effects exerted by dietary factors,intestinal microbiomes,and their metabolites on the metabolic regulation of ISCs and the potential mechanisms underlying such effects are being gradually revealed.This review summarises the effects of different dietary patterns on the behaviour and functioning of ISCs and focuses on the crosstalk between intestinal microbiota,related metabolites,and ISCs,with the aim of fully understanding the relationship between these three factors and providing further insights into the complex mechanisms associated with ISCs in the human body.Gaining an understanding of these mechanisms may lead to the development of novel dietary interventions or drugs conducive to intestinal health. | Chensi Yao Xiaowen Gou Chuanxi Tian Lijuan Zhou Rui Hao Li Wan Zeyu Wang Min Li Xiaolin Tong | 2023 | Journal of Genetics and Genomics2023，50，10： | 1 |
| 12 | Isolation and characteristics of a novel biphenyl-degrading bacterial strain,Dyella ginsengisoli LA-4显示文摘A novel biphenyl-degrading bacterial strain LA-4 was isolated from activated sludge.It was identified as Dyella ginsengisoli according to phylogenetic similarity of 16S rRNA gene sequence.This isolate could utilize biphenyl as sole source of carbon and energy,which degraded over 95 mg/L biphenyl within 36 h.The major metabolites formed from biphenyl,such as 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid(HOPDA) and benzoic acid,were identified by LC-MS.The crude cell extract of strain LA-4 exhibited the activity of 2,3-dihydroxybiphenyl 1,2-dioxygenase(2,3-DHBD) and the kinetic parameters Km was 26.48 μmol/L and Vmax was 8.12 U/mg protein.A conserved region of the biphenyl dioxygenase gene bphA1 of strain LA-4 was amplified by PCR and confirmed by DNA sequencing. | LI Ang,QU Yuanyuan,ZHOU Jiti,GOU Min School of Environmental and Biological Science and Technology,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education,China),Dalian University of Technology,Dalian 116024,China. | 2009 | Journal of Environmental Sciences2009，21，2： | 1 |
| 13 | Data Hiding in Craves with Application to Fingerprinting Maps显示文摘 | GOU Hong-mei MIN Wu | 2005 | IEEE Transactions on Signal Processing2005，53，10： | 1 |
| 14 | Knockdown of eyelophilin A reverses pa- clitaxel resistance in human endometrial cancer cells via sup- pression of MAPK kinase pathways 显示文摘 | Li Z Min W Gou J | 2013 | Cancer Chemother Pharmacol2013，72，5： | 1 |
| 15 | Data hiding in curves with application to finger-printing maps显示文摘 | Gou Hong-mei Wu Min | 2005 | IEEE Transactions on Signal Processing2005，53，10： | 1 |
| 16 | Intrinsic sensor noise features for forensic analysis on scanners and scanned images 显示文摘 | GOU Hongmei SWAMINATHAN A WU Min | 2009 | IEEE Transactions on Information Forensics and Security2009，4，3： | 1 |
| 17 | Data hiding in curves with application to fingerprinting maps 显示文摘 | Gou Hongmei Wu Min | 2005 | IEEE Trans on Signal Processing2005，53，10： | 1 |
| 18 | Knockdown of cyclophilin A reverses paclitaxel resistance in human endometrial cancer cells via suppression of MAPK kinase pathways 显示文摘 | Li Z Min W Gou J | 2013 | Cancer Chemother Pharmacol2013，72，5： | 1 |
| 19 | Regulation of NAD(H) pool and NADH/NAD + ratio by overexpression of nicotinic acid phosphoribosyltransferase for succinic acid production in Escherichia coli NZN111显示文摘 | Liya Liang Rongming Liu Guangming Wang Dongmei Gou Jiangfeng Ma Kequan Chen Min Jiang Ping Wei Pingkai Ouyang | 2012 | Enzyme and Microbial Technology2012，，5： | 1 |
| 20 | Intrinsic sen-sor noise features for forensic analysis on scanners andscanned images 显示文摘 | GOU H SWAMINATHAN A WU Min | 2009 | IEEE Trans on Information Foren-sics and Security2009，4，3： | 1 |
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