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| 1 | Regulatory T cells in patients with inflammatory bowel diseases treated with adacolumn granulocytapheresis显示文摘AIM: To investigate if the clinical efficacy of granulocytes and monocytes by adsorption (GMA) is associated with an increased frequency of peripheral regulatory T cells (Tregs), as these cells have proven to be successful in suppressing inflammatory bowel disease (IBD) in animal models. METHODS: We report four cases of corticosteroid- dependent ulcerative colitis (UC) and two Crohn’s disease (CD) cases with severe cutaneous lesions who received GMA therapy. The frequency of CD4+ CD25high (Tregs) in peripheral blood was analyzed by flow cytometry and the expression of FoxP3 and TGF beta in purified CD4+ T cells was determined by real time PCR prior to and one month after the last apheresis session, and at the time of endoscopic and clinical assessing. RESULTS: Increased expression of Fox P3 mRNA was found in all five patients who responded to cytapheresis with remission of clinical symptoms, mucosal inflammation and cutaneous lesions, and an increased frequency of circulating Tregs was found in four patients. These changes were not observed in the patient with UC who did no respond to GMA. Variations in TGF-β (mRNA) did not parallel that of FoxP3 mRNA. CONCLUSION: The clinical efficacy of GMA on IBD and related extra intestinal manifestations was associated with an expansion of circulating CD4+ CD25+ Tregs and higher expression of FoxP3 in CD4+ T cells. Accordingly, an elevated CD4+ CD25+ FoxP3 may be a valuable index of remission in patients with IBD and other chronic relapsing-remitting inflammatory conditions during treatment with GMA. | Emilio Cuadrado Marta Alonso Maria Dolores de Juan Pilar Echaniz Juan Ignacio Arenas | 2008 | World Journal of Gastroenterology2008，14，10： | 6 |
| 2 | Arachidyl amido cholanoic acid improves liver glucose and lipid homeostasis in nonalcoholic steatohepatitis via AMPK and mTOR regulation显示文摘BACKGROUND Arachidyl amido cholanoic acid(Aramchol)is a potent downregulator of hepatic stearoyl-CoA desaturase 1(SCD1)protein expression that reduces liver triglycerides and fibrosis in animal models of steatohepatitis.In a phase IIb clinical trial in patients with nonalcoholic steatohepatitis(NASH),52 wk of treatment with Aramchol reduced blood levels of glycated hemoglobin A1c,an indicator of glycemic control.AIM To assess lipid and glucose metabolism in mouse hepatocytes and in a NASH mouse model[induced with a 0.1%methionine and choline deficient diet(0.1MCD)]after treatment with Aramchol.METHODS Isolated primary mouse hepatocytes were incubated with 20μmol/L Aramchol or vehicle for 48 h.Subsequently,analyses were performed including Western blot,proteomics by mass spectrometry,and fluxomic analysis with 13C-uniformly labeled glucose.For the in vivo part of the study,male C57BL/6J mice were randomly fed a control or 0.1MCD for 4 wk and received 1 or 5 mg/kg/d Aramchol or vehicle by intragastric gavage for the last 2 wk.Liver metabolomics were assessed using ultra-high-performance liquid chromatography-time of flight-MS for the determination of glucose metabolism-related metabolites.RESULTS Combination of proteomics and Western blot analyses showed increased AMPK activity while the activity of nutrient sensor mTORC1 was decreased by Aramchol in hepatocytes.This translated into changes in the content of their downstream targets including proteins involved in fatty acid(FA)synthesis and oxidation[PACCα/β(S79),SCD1,CPT1A/B,HADHA,and HADHB],oxidative phosphorylation(NDUFA9,NDUFB11,NDUFS1,NDUFV1,ETFDH,and UQCRC2),tricarboxylic acid(TCA)cycle(MDH2,SUCLA2,and SUCLG2),and ribosome(P-p70S6K[T389]and P-S6[S235/S236]).Flux experiments with 13Cuniformely labeled glucose showed that TCA cycle cataplerosis was reduced by Aramchol in hepatocytes,as indicated by the increase in the number of rounds that malate remained in the TCA cycle.Finally,liver metabolomic analysis showed that glucose homeostasis was improved by Aramchol in 0.1MCD fed mice in a dose-dependent manner,showing normalization of glucose,G6P,F6P,UDP-glucose,and Rbl5P/Xyl5P.CONCLUSION Aramchol exerts its effect on glucose and lipid metabolism in NASH through activation of AMPK and inhibition of mTORC1,which in turn activate FAβ-oxidation and oxidative phosphorylation. | David Fernández-Ramos Fernando Lopitz-Otsoa Laura Delacruz-Villar Jon Bilbao Martina Pagano Laura Mosca Maider Bizkarguenaga Marina Serrano-Macia Mikel Azkargorta Marta Iruarrizaga-Lejarreta Jesús Sot Darya Tsvirkun Sebastiaan Martijn van Liempd Felix M Goni Cristina Alonso María Luz Martínez-Chantar Felix Elortza Liat Hayardeny Shelly C Lu JoséM Mato | 2020 | World Journal of Gastroenterology2020，26，34： | 5 |
| 3 | Antimicrobial susceptibility testing before first-line treatment for Helicobacter pylori infection in patients with dual or triple antibiotic resistance显示文摘AIM To evaluate the efficacy of antimicrobial susceptibilityguided therapy before first-line treatment for infection in patients with dual or triple antibiotic resistance.METHODS A total of 1034 patients infected by Helicobacter pylori(H. pylori) during 2013-2014 were tested for antimicrobial susceptibility. 157 of 1034(15%) patients showed resistance to two(127/1034; 12%) and to three(30/1034; 3%) antibiotics. Sixty-eight patients with dual H. pylori-resistance(clarithromycin, metronidazole or levofloxacin) were treated for 10 d with triple therapies: OAL(omeprazole 20 mg b.i.d., amoxicillin 1 g b.i.d., and levofloxacin 500 mg b.i.d.) 43cases, OAM(omeprazole 20 mg b.i.d., amoxicillin 1 g b.i.d., and metronidazole 500 mg b.i.d.) 12 cases and OAC(omeprazole 20 mg b.id., amoxicillin 1 g b.i.d., and clarithromycin 500 mg b.i.d.) 13 cases based on the antimicrobial susceptibility testing. Twelve patients showed triple H. pylori-resistance(clarithromycin, metronidazole and levofloxacin) and received for 10 d triple therapy with OAR(omeprazole 20 mg b.id., amoxicillin 1 g b.i.d., and rifabutin 150 mg b.i.d.). Eradication was confirmed by 13C-urea breath test. Adverse effects and compliance were assessed by a questionnaire. RESULTS Intention-to-treat eradication rates were: OAL(97.6%), OAM(91.6%), OAC(92.3%) and OAR(58.3%). Cure rate was significantly higher in na?ve patients treated with OAR-10 compared to patients who had two or three previous treatment failures(83% vs 33%). Adverse events rates for OAL, OAM, OAC and OAR were 22%, 25%, 23% and 17%, respectively, all of them mild-moderate. CONCLUSION Antimicrobial susceptibility-guided triple therapies during 10 d for first-line treatment leads to an eradication rate superior to 90% in patients with dual antibiotic H. pylori resistance. | Angel Cosme Milagrosa Montes Begona Ibarra Esther Tamayo Horacio Alonso Usua Mendarte Jacobo Lizasoan Marta Herreros-Villanueva Luis Bujanda | 2017 | World Journal of Gastroenterology2017，23，18： | 4 |
| 4 | Nested PCR improves detection of infectious hematopoietic necrosis virus in cells coinfected with infectious pancreatic necrosis virus显示文摘 | Marta Alonso Sylvia Rodr??guez Sara I Pérez Prieto | 1999 | Journal of Virological Methods1999，，1： | 2 |
| 5 | Development of a suicidal DNA vaccine for infectious hematopoietic necrosis virus (IHNV)显示文摘 | Marta Alonso Peter P. Chiou Jo-Ann Leong | 2011 | Fish and Shellfish Immunology2011，，3： | 1 |
| 6 | Effects of S-adenosylmethionine on lipid peroxidation and liver fibrogenesis in carbon tetrachloride-induced cirrhosis显示文摘 | Marta Gassó Mireia Rubio Gregorio Varela María Cabré Joan Caballería Elena Alonso Ramón Deulofem Jordi Camps América Giménez María Pajares Albert Parés José M. Mato Joan Rodés | 1996 | Journal of Hepatology1996，，2： | 1 |
| 7 | Consequence analysis by means of characteristic curves to determine the damage to build- ings from the detonation of explosive substances as a function of TNT equivalence 显示文摘 | Fernando Diaz Alonso Enrique Gonzalez Ferradas Marta Doval Minarro | 2008 | Journal of Loss Prevention in the Process Industries2008，，21： | 1 |
| 8 | Suppressive Subtraction Libraries to Identify Interferon-Inducible Genes in Fish显示文摘 | Marta Alonso Jo-Ann Leorg | 2002 | Mar Biotechnol2002，4，： | 1 |
| 9 | Proton magnetic resonance spectroscopy (1H MRS) of human brain tumours: assessment of differences between tumour types and its applicability in brain tumour categorization显示文摘 | Carles Majós Juli Alonso Carles Aguilera Marta Serrallonga Javier Pérez-Martín Juan J. Acebes Carles Arús Jaume Gili | 2003 | European Radiology2003，，3： | 1 |
| 10 | Atorvastatin Prevents Angiotensin II–Induced Vascular Remodeling and Oxidative Stress显示文摘 | Ana M. Briones Natalia Rodríguez-Criado Raquel Hernanz Ana B. García-Redondo Raul R. Rodrigues-Díez María J. Alonso Jesús Egido Marta Ruiz-Ortega Mercedes Salaices | 2009 | Hypertension2009，，1： | 1 |
| 11 | Atorvastatin Prevents Angiotensin II–Induced Vascular Remodeling and Oxidative Stress显示文摘 | Ana M. Briones Natalia Rodríguez-Criado Raquel Hernanz Ana B. García-Redondo Raul R. Rodrigues-Díez María J. Alonso Jesús Egido Marta Ruiz-Ortega Mercedes Salaices | 2009 | Hypertension2009，，： | 1 |
| 12 | Annexin‐A2 as predictor biomarker of recurrent disease in endometrial cancer显示文摘 | Lorena Alonso‐Alconada Maria Santacana Pablo Garcia‐Sanz Laura Muinelo‐Romay Eva Colas Cristina Mirantes Marta Monge Juan Cueva Esther Oliva Robert A Soslow Maria Angeles Lopez Jose Palacios Jaime Prat Joan Valls Camilla Krakstad Helga Salvesen Antonio Gi | 2015 | Int. J. Cancer2015，，： | 1 |
| 13 | Proton magnetic resonance spectroscopy (1H MRS) of human brain tumours: assessment of differences between tumour types and its applicability in brain tumour categorization显示文摘 | Carles Majós Juli Alonso Carles Aguilera Marta Serrallonga Javier Pérez-Martín Juan J. Acebes Carles Arús Jaume Gili | 2003 | European Radiology2003，，3： | 1 |
| 14 | Consequence analysis by meansof characteristic curves to determine the damage tobuildings from the detonation of explosive substances asa function of TNT equivalence显示文摘 | Fernando Diaz Alonso Enrique Gonzalez Ferradas Marta Doval Minarro | 2008 | Journal of LossPrevention in the Process Industries2008，，21： | 1 |
| 15 | Suppressive subtraction libraries to identify interferon-inducible genes in fish 显示文摘 | Marta Alonso Jo-Ann Leong | 2002 | Mar Biotechnol2002，4，： | 1 |
| 16 | New insights into brain glutaminases: Beyond their role on glutamatergic transmission显示文摘 | Javier Márquez Marta Tosina Vanessa de la Rosa Juan A. Segura Francisco J. Alonso José M. Matés José A. Campos-Sandoval | 2009 | Neurochemistry International2009，，1： | 1 |
| 17 | Randomized Controlled Trial of Aspiration Needle versus Automated Biopsy Device for Transjugular Liver Biopsy显示文摘 | Rafael Ba?ares Sonia Alonso María-Vega Catalina Marta Casado Diego Rincón Magdalena Salcedo Emilio Alvarez Carmen Guerrero Antonio Echenagusía Fernando Camú?ez Gonzalo Simó | 2001 | Journal of Vascular and Interventional Radiology2001，，5： | 1 |
| 18 | Lipoprotein(a) levels in Familial Hipercholesterolaemia: an important predictor for cardiovascular disease independent of the type of LDL-receptor mutation显示文摘 | Rodrigo Alonso Eduardo Andres Nelva Mata Francisco Fuentes-Jiménez Lina Badimón José López-Miranda Teresa Padró Ovidio Mu?iz Jose Luis Díaz-Díaz Marta Mauri Jose María Ordovás Pedro Mata Rocío Aguado | 2014 | Journal of the American College of Cardiology2014，，： | 1 |
| 19 | Molecular cloning, characterization and tissue expression of porcine Toll-like receptor 4显示文摘 | Belén álvarez Concepción Revilla Sonia Chamorro Marta López-Fraga Fernando Alonso Javier Domínguez Angel Ezquerra | 2005 | Developmental and Comparative Immunology2005，，4： | 1 |
| 20 | Constructive thinking,rational intelligence and irritable bowel syndrome显示文摘AIM:To evaluate rational and experiential intelligence in irritable bowel syndrome (IBS) sufferers.METHODS:We recruited 100 subjects with IBS as per Rome criteria(50 consulters and 50 non-consulters) and 100 healthy controls,matched by age,sex and educational level.Cases and controls completed a clinical questionnaire(including symptom characteristics and medical consultation)and the following tests:rational-intelligence(Wechsler Adult Intelligence Scale,3rd edition);experiential intelligence(Constructive Thinking Inventory);personality(NEO personality inventory); psychopathology(MMPI-2),anxiety(state-trait anxiety inventory)and life events(social readjustment rating scale).Analysis of variance was used to compare the test results of IBS-sufferers and controls,and a logistic regression model was then constructed and adjusted for age,sex and educational level to evaluate any possible association with IBS.RESULTS: No differences were found between IBS cases and controls in terms of IQ (102.0 ±10.8 vs 102.8±12.6), but IBS sufferers scored significantly lower in global constructive thinking (43.7±9.4 vs 49.6±9.7). In the logistic regression model, global constructive thinking score was independently linked to suffering from IBS [OR 0.92 (0.87-0.97)], without significant OR for total IQ.CONCLUSION: IBS subjects do not show lower rational intelligence than controls, but lower experiential intelligence is nevertheless associated with IBS. | Enrique Rey Marta Moreno Ortega Monica Olga Garcia Alonso Manuel Diaz-Rubio | 2009 | World Journal of Gastroenterology2009，15，25： | 0 |
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